Birendra Prasad
@pup.ac.in
PROFESSOR Botany/Science
Patna University
EDUCATION
M.Sc., Ph.D.
RESEARCH INTERESTS
Antimicrobial Secondary metabolite, Anti-aging plant metabolite, Bioethanol production from agro-wastes
Scopus Publications
Scopus Publications
Nikhat Parween, Amber Jabeen, and Birendra Prasad
Bentham Science Publishers Ltd.
Aim: To analyze the efficacy of eugenol on longevity by assessing its antioxidant effect using Caenorhabditis elegans as an animal model. Background: Eugenol is a major polyphenolic component of Ocimum sanctum (Tulsi) which attributes wide pharmacological activities and can serve as a biomarker. However, the possible effect of eugenol on longevity in Caenorhabditis elegans has not been reported. Objective: The objective of this investigation was to provide the first scientific based results about the effect of eugenol on longevity, slowing down of paralysis in Alzheimer’s model and the mechanism behind it in Caenorhabditis elegans animal model system. Methods: The phenolic components of methanolic extract of Ocimum sanctum were analyzed by RP-HPLC. Worms were exposed to different concentrations of extract and one of its components - eugenol. Lifespan, health span, survival in CL4176 Alzheimer’s model and molecular mechanism were analyzed. Results: Extract of Ocimum sanctum and eugenol increased lifespan and provided indemnity against pro-oxidants. It also significantly improved healthy ageing and slowed the progression of neurodegeneration in CL4176 Alzheimer’s model of the worm by increasing survival against prooxidants and slowing down the paralysis. Longevity effect was independent of the DAF-16 as observed by using DAF-16::GFP and daf-16 null mutant strains. These results implicate eugenol as a potent therapeutic compound that may curtail ageing and age related disorders like- Alzheimer’s disease. Conclusion: The present work demonstrated eugenol as a potential anti-ageing compound that may curtail ageing, improve heath span by enhancing resistance to oxidative stress and exerts its effect independent of DAF-16 pathway. So, it can be assumed that eugenol can be beneficial to humans as well, albeit further research is necessary before declaring it for human consumption.
Amber Jabeen, Nikhat Parween, Kumar Sayrav, and Birendra Prasad
Elsevier BV
Kumar Pranay, Shree Ram Padmadeo, and Birendra Prasad
Elsevier BV
K. Pranay, S. R. Padmadeo, V. Jha and B. Prasad
Journal of Applied Pharmaceutical Science
An attempt was made to isolate and screen efficient amylolytic strains of Bacillus sp. Initial screening based on Starch hydrolysis ratio (SHR) resulted in selection of 72 amylolytic bacterial strains. Among these, 18 strains were selected for further studies. Secondary screening based on amylase production in starch broth medium led to selection of six amylolytic strains of Bacillus sp. The selected strains were grown in four different fermentation media (FMI-FMIV) in order to screen for three most efficient amyloytic strains for optimization and characterization. FMIV was the best basal medium for amylase production as it had the shortest incubation time (24 hours) and highest amylase production. Molecular identification based on 16S rDNA sequence revealed that three most efficient strains (BCM36 (KR1), BCM33 (KR2) & BCM25 (KR3)) belonged to Bacillus sp.
Niraj K. Singh, Ravi R. Sonani, Anjali Awasthi, Birendra Prasad, Anjali R. Patel, Jitendra Kumar, and Datta Madamwar
Springer Science and Business Media LLC
Jitendra Kumar, Kyung-Chae Park, Anjali Awasthi, and Birendra Prasad
Bentham Science Publishers Ltd.
Aging is a process of progressive decline in physiological functions resulting in increased vulnerability to diseases and death. Aging results in increased rates of age related disorders like neurodegenerative diseases, cardiovascular diseases, diabetes, cancer, arthritis etc. Modulation of insulin signaling, protein aggregation, stress, free radical damage and inflammation are the major causes for deleterious changes resulting in aging. Many studies are being undertaken to find novel compounds which can improve a typical human life span and aid in healthy aging. We investigated the potential of one such compound silymarin for its anti-aging effect. Silymarin is a flavanone derivative extracted from the seeds of the milk thistle Silybum marianum. It is widely used for the treatment of liver diseases in clinical practice. We tested the anti-aging efficacy of silymarin using the Caenorhabditis elegans model system. Our results demonstrate that C. elegans treated with 25μM and 50μM silymarin concentration resulted in an increase in mean lifespan by 10.1% and 24.8% respectively compared to untreated control. Besides increased lifespan, silymarin treated aged animals showed better locomotion rate, higher response to stimuli and improved tolerance to stress compared to untreated control. We also checked the potential of silymarin to slow the progression of neurodegenerative disorder like Alzheimer's disease (AD) by using CL4176 C. elegans model for AD. C. elegans CL4176 transgenic animal induces expression of amyloid beta-protein (Aβ1-42) in muscle tissues when subjected to temperature of 23°C and above resulting in worm paralysis. CL4176 animals treated with silymarin showed delayed paralysis via enhancing resistance to oxidative stress. These results suggested that silymarin is a potential hormetin for preventing aging and age-related diseases.
Ravi Raghav Sonani, Niraj Kumar Singh, Anjali Awasthi, Birendra Prasad, Jitendra Kumar, and Datta Madamwar
Springer Science and Business Media LLC
N. Kumari, P. Kumar, D. Mitra, B. Prasad, B.N. Tiwary, and L. Varshney
Wiley
Triphala, a mixture of Emblica officinalis, Terminalia chebula, and Terminalia bellirica, containing ingredients from plant origin, is often prone to microbial contamination. A high level of microbial contamination was observed in Triphala samples obtained from different sources. On gamma radiation processing, a sharp decline in log CFU was observed with increasing radiation dose and a complete decontamination at 5 kGy. Average D10 value for total aerobic and fungal counts were observed to be 0.55 +/- 0.073 kGy and 0.94 +/- 0.043 kGy, respectively. Water extracts of irradiated samples showed linearly increasing concentration of gallic acid (3.3 to 4.5 times), total phenolic contents (2.16 to 2.87 times), and antioxidant properties with increasing radiation dose up to 25 kGy. The increase could be attributed to easy release of active ingredients from their radiation degraded complex forms. Aflatoxin B(1) and ochratoxin could not be detected in the samples. Gamma-radiation dose up to 5 kGy could be safely used to hygienize Triphala.
Rajesh Anand, Birendra Prasad, and Bhupendra Nath Tiwary
Springer Science and Business Media LLC
Bhupendra N. Tiwary, Birendra Prasad, Anuradha Ghosh, Sanjay Kumar, and Rakesh K. Jain
Springer Science and Business Media LLC