Sensors in Livestock Production; Male reproductive management
29
Scopus Publications
641
Scholar Citations
13
Scholar h-index
18
Scholar i10-index
Scopus Publications
Single nucleotide polymorphisms cumulating to genetic variation for fertility in crossbred (Bos taurus × Bos indicus) bull spermatozoa Manish Kumar Sinha, Arumugam Kumaresan, Thirumala Rao Talluri, John Peter Ebenezer Samuel King, Mani Arul Prakash, Pradeep Nag, Nilendu Paul, Kathan Raval, Elango Kamaraj, Aranganathan V Animal Biotechnology, 2023 Spermatozoa from high-fertile (HF) and low-fertile (LF) breeding bulls were subjected to high-throughput next-generation sequencing to identify important Single nucleotide polymorphisms (SNPs) and novel variants associated with fertility. A total of 77,038 genome-wide SNPs were identified, among which, 10,788 were novel variants. A total of 42,290 and 34,748 variants were recorded with 6115 and 4673 novel variants in in HF and LF bulls, respectively. Higher number of SNPs were identified in HF compared to LF bulls. GO analysis of filtered genes with significant variations in HF bulls indicated their involvement in oxidative phosphorylation and metabolic pathways. GO analysis of filtered genes with significant variation in LF bulls revealed their involvement in Ca2++ ion binding, structural constituent of ribosome, and biological processes like translation and ribosomal small subunit assembly. The study identified SNPs in candidate genes including TPT1, BOLA-DRA, CD74, RPS17, RPS28, RPS29, RPL14, RPL13, and RPS27A, which are linked to sperm functionality, survival, oxidative stress, and bull fertility. The identified SNPs could be used in selection of bulls for high fertility and the variation in these genes could be established as an explanation for the fertility differences in bulls upon validation in large number of bulls.
Cryopreservation process alters the expression of genes involved in pathways associated with the fertility of bull spermatozoa John Peter Ebenezer Samuel King, Manish Kumar Sinha, Arumugam Kumaresan, Pradeep Nag, Mohua Das Gupta, Mani Arul Prakash, Thirumala Rao Talluri, Tirtha Kumar Datta Frontiers in Genetics, 2022 In bovines, cryopreserved semen is used for artificial insemination; however, the fertility of cryopreserved semen is far lower than that of fresh semen. Although cryopreservation alters sperm phenotypic characteristics, its effect on sperm molecular health is not thoroughly understood. The present study applied next-generation sequencing to investigate the effect of cryopreservation on the sperm transcriptomic composition of bull spermatozoa. While freshly ejaculated bull spermatozoa showed 14,280 transcripts, cryopreserved spermatozoa showed only 12,375 transcripts. Comparative analysis revealed that 241 genes were upregulated, 662 genes were downregulated, and 215 genes showed neutral expression in cryopreserved spermatozoa compared to fresh spermatozoa. Gene ontology analysis indicated that the dysregulated transcripts were involved in nucleic acid binding, transcription-specific activity, and protein kinase binding involving protein autophosphorylation, ventricular septum morphogenesis, and organ development. Moreover, the dysregulated genes in cryopreserved spermatozoa were involved in pathways associated with glycogen metabolism, MAPK signalling, embryonic organ morphogenesis, ectodermal placode formation, and regulation of protein auto-phosphorylation. These findings suggest that the cryopreservation process induced alterations in the abundance of sperm transcripts related to potential fertility-associated functions and pathways, which might partly explain the reduced fertility observed with cryopreserved bull spermatozoa.
Comparative Transcriptomic Analysis of Spermatozoa From High- and Low-Fertile Crossbred Bulls: Implications for Fertility Prediction Mani Arul Prakash, Arumugam Kumaresan, John Peter Ebenezer Samuel King, Pradeep Nag, Ankur Sharma, Manish Kumar Sinha, Elango Kamaraj, Tirtha Kumar Datta Frontiers in Cell and Developmental Biology, 2021 Crossbred bulls produced by crossing Bos taurus and Bos indicus suffer with high incidence of infertility/subfertility problems; however, the etiology remains poorly understood. The uncertain predictability and the inability of semen evaluation techniques to maintain constant correlation with fertility demand for alternate methods for bull fertility prediction. Therefore, in this study, the global differential gene expression between high- and low-fertile crossbred bull sperm was assessed using a high-throughput RNA sequencing technique with the aim to identify transcripts associated with crossbred bull fertility. Crossbred bull sperm contained transcripts for 13,563 genes, in which 2,093 were unique to high-fertile and 5,454 were unique to low-fertile bulls. After normalization of data, a total of 776 transcripts were detected, in which 84 and 168 transcripts were unique to high-fertile and low-fertile bulls, respectively. A total of 176 transcripts were upregulated (fold change > 1) and 209 were downregulated (<1) in low-fertile bulls. Gene ontology analysis identified that the sperm transcripts involved in the oxidative phosphorylation pathway and biological process such as multicellular organism development, spermatogenesis, and in utero embryonic development were downregulated in low-fertile crossbred bull sperm. Sperm transcripts upregulated and unique to low-fertile bulls were majorly involved in translation (biological process) and ribosomal pathway. With the use of RT-qPCR, selected sperm transcripts (n = 12) were validated in crossbred bulls (n = 12) with different fertility ratings and found that the transcriptional abundance of ZNF706, CRISP2, TNP2, and TNP1 genes was significantly (p < 0.05) lower in low-fertile bulls than high-fertile bulls and was positively (p < 0.05) correlated with conception rate. It is inferred that impaired oxidative phosphorylation could be the predominant reason for low fertility in crossbred bulls and that transcriptional abundance of ZNF706, CRISP2, TNP2, and TNP1 genes could serve as potential biomarkers for fertility in crossbred bulls.
Environmental factors affecting reproductive performance of Murrah buffaloes V. Boopathi, Shiv Prasad, A. Kumaresan, A. Manimaran, M. Arul Prakash Biological Rhythm Research, 2021 Buffalo is the backbone of the farmer’s economy in Indian dairy industry. Data from 288 Murrah buffalo female calves maintained at the Livestock Research Center, Karnal, Haryana, born in the period between 2000 and 2009 were used for this study. Data were analysed using least-square procedures. Results indicated that season and period of birth had significant (P < 0.05) effect on reproductive performance of Murrah buffalo heifers. However, there was no significant effect of birth weight on different reproductive performance. The phenotypic parameter estimates indicated that the season and birth weight of a calf can be used as a selection tool for improvement of reproductive traits considering its high positive phenotypic correlations with succeeding reproductive traits.
RNA-Seq analysis reveals functionally relevant coding and non-coding RNAs in crossbred bull spermatozoa Mani Arul Prakash, Arumugam Kumaresan, Manish Kumar Sinha, Elango Kamaraj, Tushar Kumar Mohanty, Tirtha Kumar Datta, Jane M. Morrell Animal Reproduction Science, 2020 Sperm, which are believed to be transcriptionally and translationally inactive, synthesize RNA and proteins before there is gradual disappearance of the ribosome during chromatin compaction. Sperm transfer several functionally relevant transcripts to the oocyte, controlling maternal-zygotic transition and embryonic development. The present study was undertaken to profile and analyze sperm transcripts comprehensively using Next Generation Ribonucleic acid sequencing technology in Holstein Friesian x Tharparkar crossbred bulls. The results from global transcriptomic profiling revealed transcripts for 13,814 genes; of which 431 transcripts were expressed with >1 FPKM and 13,383 transcripts were expressed with >0 or <1 FPKM. The abundant mRNA transcripts of crossbred bull sperm were PRM1 and HMGB4. Gene ontology of transcripts with>1 FPKM revealed there was a major involvement in the structural constituent of ribosomes and translation. Results from pathway enrichment indicated the connection between ribosome, oxidative phosphorylation and spliceosome pathways and the transcripts of crossbred bull spermatozoa. The transcriptional abundance of selected genes, validated using RT-qPCR, indicated significant variations between bulls. Collectively, it may be inferred that the transcripts in crossbred bull sperm were heavily implicated in functions such as the structural constituent of ribosomes and translation, and pathways such as ribosome, oxidative phosphorylation and spliceosome. Further studies using larger sample sizes are required to understand the possible implications of transcriptomic variations on semen quality and fertility.
Evaluation of indirect diagnostic tests and PBMC expression of innate immune genes in subclinical mastitis in dairy cows Indian Journal of Animal Sciences, 2020
Sub-fertility in crossbred bulls: Deciphering testicular level transcriptomic alterations between zebu (Bos indicus) and crossbred (Bos taurus x Bos indicus) bulls Kamaraj Elango, Arumugam Kumaresan, Ankur Sharma, Pradeep Nag, Mani Arul Prakash, Manish Kumar Sinha, Ayyasamy Manimaran, Ebenezer Samuel King John Peter, Sakthivel Jeyakumar, Sellappan Selvaraju, Kerekoppa P. Ramesha, Tirtha K. Datta BMC Genomics, 2020 Background The incidence of poor semen quality and sub-fertility/infertility is higher in crossbred as compared to Zebu males. Several attempts have been made to understand the possible reasons for higher incidence of fertility problems in crossbred males, at sperm phenotype, proteome and genome level but with variable results. Since the quality of the ejaculated spermatozoa is determined by the testicular environment, assessing the testicular transcriptome between these breeds would help in identifying the possible mechanisms associated with infertility in crossbred bulls. However, such information is not available. We performed global transcriptomic profiling of testicular tissue from crossbred and Zebu bulls using Agilent Bos taurus GXP 8X60k AMADID: 29411 array. To the best of our knowledge, this is the first study comparing the testicular mRNAs between crossbred and Zebu bulls. Results Out of the 14,419 transcripts detected in bovine testis, 1466 were differentially expressed between crossbred and Zebu bulls, in which 1038 were upregulated and 428 were downregulated in crossbred bulls. PI4KB and DPY19L2 genes, reported to be involved in sperm capacitation and acrosome formation respectively, were among the top 10 downregulated transcripts in crossbred testis. Genes involved in ubiquitination and proteolysis were upregulated, while genes involved in cell proliferation, stem cell differentiation, stem cell population maintenance, steroidogenesis, WNT signalling, protein localization to plasma membrane, endocannabinoid signalling, heparin binding, cAMP metabolism and GABA receptor activity were downregulated in crossbred testis. Among the 10 genes validated using qPCR, expression of CCNYL, SOX2, MSMB, SPATA7, TNP1, TNP2 and CRISP2 followed the same trend as observed in microarray analysis with SPATA7 being significantly downregulated and transition proteins (TNP1, TNP2) being significantly upregulated in crossbred bulls. Conclusions Abundant proteolysis by ubiquitination and downregulation of WNT signaling, cell proliferation, differentiation and steroidogenesis might be associated with higher incidence of poor semen quality and/or sub-fertility/infertility in crossbred bulls as compared to Zebu bulls. Downregulation of SPATA7 (Spermatogenesis Associated 7) and upregulation of transition proteins (TNP1 and TNP2) in crossbred bull testis might be associated with impaired spermatogenesis processes including improper chromatin compaction in crossbred bulls.
Influence of uterine infection on outcome of prostaglandin F2α treatment in dairy animals Indian Veterinary Journal, 2015
Effects of clinical mastitis on days open in dairy cattle and buffaloes Indian Veterinary Journal, 2014
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Advanced Ceramics for Antimicrobial Applications M Prakash, NS Kumar, KCB Naidu Applications of Advanced Ceramics in Science, Technology, and Medicine, 157-169 , 2020 2020
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Infrared thermography: A potential noninvasive tool to monitor udder health status in dairy cows M Sathiyabarathi, S Jeyakumar, A Manimaran, G Jayaprakash, ... Veterinary world 9 (10), 1075 , 2016 2016 Citations: 111
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Sexing of spermatozoa in farm animals: a mini review. MA Prakash, AK Arumugam Kumaresan, AM Ayyasamy Manimaran, ... 2014 Citations: 35
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