Evandro Neves Silva

He has a Technical Course in Clinical Analysis (2015), with an emphasis on Biochemistry and Hematology from the Federal Institute of Education, Science and Technology of the South of Minas Gerais - IFSULDEMINAS/Muzambinho, Graduated in Biomedicine (2017) from the José do Rosário Vellano University - UNIFENAS/ Alfenas, with emphasis on Microbiology. Master's degree completed (2019) by the Graduate Program in Reproduction, Health and Animal Welfare, working in Microbiology and Immunology. He is currently a PhD candidate at the same program, with an emphasis on Immunology and Genetics (Molecular Biology). He is a permanent member of the Brazilian Society of Immunology (SBI), the Society for Leukocyte Biology (SLB) and the Nucleus of Studies in Assisted Human Reproduction (NERHA), collaborator in research projects at the Department of Microbiology and Immunology of the Federal University of Alfenas - UNIFAL and researcher/PhD student at the Laboratory of Molecular and Cellular Biology.

RESEARCH INTERESTS

- Gut microbiota
- Host-pathogen infection
- Immunotherapy
- Murine reproduction
- Molecular Biology of domestic animals

Scopus Publications

Omega-3 modulates macrophage immunometabolic profile without changing Pseudomonas aeruginosa proliferation
Priscila Vilela Sousa, Evandro Neves Silva, Leonardo Pereira de Araújo, Ana Clara Moreira, Fernanda Silva Carneiro, Yasmin Vieira Braida, Tatiana Teixeira de Miranda, Caio Tavares Fagundes, Lirlândia Pires de Sousa, Leonardo Augusto de Almeida, Patrícia Paiva Corsetti
Life Sciences, 2026
The increasing and often indiscriminate use of medications challenges populations with limited access to effective treatments. Natural compounds such as omega-3 fatty acids (FA), including eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), exert immunomodulatory effects by incorporating into macrophage membranes and modulating cytokine production and metabolism. This study evaluated the effects of omega-3 FA on inflammatory responses, metabolic reprogramming, and signaling pathways in bone marrow-derived macrophages (BMDMs) infected with Pseudomonas aeruginosa. BMDMs from C57BL/6 mice were pretreated for 3 h with 26 mg DHA and 36 mg EPA, then stimulated with P. aeruginosa (PA14), heat-killed bacteria (HKPA), or lipopolysaccharide (LPS). Omega-3 FA pretreatment reduced pro-inflammatory mediators, including TNF-α, IL-1β, nitric oxide, and NLRP3 expression in macrophages stimulated with PA14, HKPA and LPS, while IL-10 secretion was also reduced under these stimuli. However, omega-3 FA increased IL-10 production under basal conditions. Gene expression analysis revealed upregulation of M2-associated markers (ARG1 and CD206), without changes in the M1 marker iNOS. Metabolic and transcriptional analyses demonstrated downregulation of glycolysis-related genes and upregulation of genes associated with mitochondrial function and lipid metabolism. Omega-3 FA did not alter P. aeruginosa proliferation but increased bacteria phagocytosis. Molecular docking suggested that omega-3 FA interact with key inflammatory targets, including GPR120 and PPARγ. Consistently, pharmacological antagonism of PPARγ reversed the omega-3 FA suppressive effects on TNF-α and IL-1β production and NLRP3 expression. These results indicate that omega-3 FA modulates macrophage immune responses and metabolism during P. aeruginosa infection, promoting an anti-inflammatory, metabolically adapted phenotype without impairing bacterial clearance.
Faecal Microbiota Transplantation in IL-10 Knockout Mice Reverses Increased Susceptibility to Pseudomonas aeruginosa Lung Infection
Natália Cristina de Melo Santos, Evandro Neves Silva, Leonardo Pereira de Araújo, Carlos Roberto Prudêncio, Rômulo Dias Novaes, Patrícia Paiva Corsetti, Leonardo Augusto de Almeida
Microbiology Research, 2026
Differences in the gut microbiota are directly reflected in lung–gut axis crosstalk, which may increase susceptibility to pulmonary infections, such as those caused by the bacterium Pseudomonas aeruginosa. Deficiency of the cytokine IL-10 leads to gut inflammation, and this pro-inflammatory environment is partly due to changes in the gut microbiota. To better understand the effects of IL-10 deficiency on the gut microbiota, the intestinal microbial composition of IL-10 KO mice was assessed, and an increase in the phyla Bacteroidetes and Proteobacteria and a decrease in the phylum Firmicutes were observed in the faeces compared with the wild-type group (WT). Additionally, IL-10 KO mice had a higher pro-inflammatory immunostimulatory caecal content. Furthermore, it was found that heterologous faecal microbiota transplantation (FMT) between groups reversed this gut imbalance. IL-10 KO mice showed greater susceptibility to acute pulmonary infection by P. aeruginosa, with a higher recovery of viable bacteria in the lung and spleen, greater tissue damage and increased expression of genes encoding pro-inflammatory cytokines in the lungs. This greater susceptibility was reversed after FMT. Taken together, these results demonstrate the role of endogenous IL-10 in the gut microbiota constitution and its importance in the pulmonary immune response against P. aeruginosa infection.
Targeting conserved epitopes in structural proteins: a next-generation vaccine strategy against the newly identified HKU5-CoV-2 virus
Leonardo Pereira de Araújo, Evandro Neves Silva, André Luiz Caliari Costa, Patrícia Paiva Corsetti, Leonardo Augusto de Almeida
Signal Transduction and Targeted Therapy, 2025
A recent study published in Cell by Chen et al., highlighted by Nature journal, identified the HKU5-CoV-2 lineage, a coronavirus detected in bats with the potential to interact with the human ACE2 receptor. 1 , 2 Experiments conducted in intestinal and airway cells indicated that HKU5-CoV-2 binds to the receptor less efficiently than SARS-CoV-2 . However, the broad ACE2 ortholog tropism suggests a potential risk of adaptation to the human host. 1 , 2
Multivalent vaccine candidate from conserved immunogenic peptides in entry or exit proteins of Orthopoxvirus genus
Leonardo Pereira de Araújo, Evandro Neves Silva, Severino Matias de Alencar, Patrícia Paiva Corsetti, Leonardo Augusto de Almeida
Scientific Reports, 2025
Orthopoxvirus (OPXV) genus includes emerging and re-emerging zoonotic viruses that pose threats to global health. Smallpox caused pandemics in the 20th century. Borealpox was responsible for a death in Alaska in 2024. Mpox, declared a Public Health Emergency by the WHO in 2022, with an alert reclassification in 2024. The lack of effective therapies and the limitations of attenuated virus vaccines, especially for immunocompromised individuals, reinforce the urgent need for new strategies to prevent diseases caused by pathogens of the OPXV genus. This study aimed to identify conserved epitopes in proteins essential for the entry and exit of these viruses and, based on this identification, develop a promising multivalent vaccine candidate. Viral protein sequences were extracted from the NCBI Virus database, and 160 sequences were analyzed to identify conserved epitopes using the Immune Epitope Database. After filtering the data, epitopes were concatenated to create a chimeric multi-epitope protein combined with β-defensin and PADRE adjuvants. The resulting protein, with eight conserved epitopes covering all OPXV viruses (including Mpox Clade Ib), was evaluated for antigenicity, allergenicity, and structural stability. It showed strong interaction with the TLR2 receptor, along with good predictions for immune responses after three doses. This proposed multivalent vaccine represents a potential approach against these zoonotic viruses, with promising results for in vitro and in vivo studies.
Exploring Brucella spp. genomes for CRISPR-Cas systems
Leonardo Pereira de Araújo, Augusto Rodrigues Lima, Evandro Neves Silva, Thiago Caetano Andrade Belo, Laura Leone da Silva, Leonardo Augusto de Almeida, Patrícia Paiva Corsetti
Biologia, 2025
Identification and functional annotation of candidate genes and transcription factors associated with reproductive traits to Nelore bulls: new insights from GWAS and post-GWAS analysis
Evandro Neves Silva, Thaís Cristina Ferreira Dos Santos, L. Verardo, Ana Fabrícia Braga Magalhães, Danielly Beraldo dos Santos Silva
Tropical Animal Health and Production, 2025
Amoxicillin-induced bacterial gut dysbiosis: A critical influence on mice reproduction and their offspring development
Evandro Neves Silva, Thaís Cristina Ferreira dos Santos, Vanessa Coelho Teixeira, Thiago Caetano Andrade Belo, Lara Vilela Augusto, Leonardo Augusto de Almeida, Patrícia Paiva Corsetti
Microbial Pathogenesis, 2025
Ivermectin impact over gut microbiota diversity: A comprehensive and updated analysis from pre-clinical and clinical evaluations
Thiago Caetano Andrade Belo, Evandro Neves Silva, Patrícia Paiva Corsetti, Leonardo Augusto de Almeida
Microbe Netherlands, 2025
The antiparasitic ivermectin (IVM) has been widely used in human and veterinary medicine since 1988, due to its effectiveness in combating various parasitic infections. Recently, this drug gained media attention due to its potential antiviral activity during the Covid-19 pandemic However, despite its proven ineffectiveness in treating the disease, its use increased significantly. Thus, concerns have arisen regarding the excessive use of IVM over the gut microbiota diversity, raising questions about possible gut-dysbiosis and health implications. Studies in various mammalian species have shown that oral administration of IVM can induce gut dysbiosis, whereas other administration routes do not exhibit this effect. This alteration in gut microbiota composition may potentially affect immune response. However, there are divergences between the studies, especially due to different administration protocols and animal species. Research on non-mammalian organisms, such as mosquito larvae and beetles, also indicates a potential dysbiotic effect related to the use of IVM or the reflection of this effect on species that live in the same environment of treated animals. However, the understanding of these effects and their implications for human and animal health is still limited, highlighting the urgent need for studies to elucidate the role of IVM in the gut microbiota and its impact on health. Ivermectin can induce bacterial gut dysbiosis. The use of ivermectin leads to gut dysbiosis in both mammals and non-mammals, resulting in alterations to the composition of bacterial phyla, as evidenced by studies in humans and animal models, including mice, rats, tigers, horses, deer, dogs, as well as non-mammals such as larvae and beetles. In contrast, non-oral administration in mammals did not significantly alter the gut microbiota, as observed in animal models of chinchillas and sea lions. • Oral ivermectin can modulate the mammal gut microbiota. • Ivermectin induces gut bacterial dysbiosis in non-mammalian organisms. • Non-oral ivermectin administration in mammals has limited effects on gut microbiota. • The systemic impacts of ivermectin-induced gut dysbiosis remain uncertain.
Sequential macrophage DENV and ZIKV infection shows differential expression of CD86, IFN-β, and regulation of TNF-α and IL-1β depending on DENV serotype
Gustavo Andrade Brancaglion, Gabriel Augusto Pires de Souza, Leonardo Pereira de Araújo, Evandro Neves Silva, Laura Leone da Silva, Fernanda de Lima Tana, Patrícia Paiva Corsetti, Luiz Felipe Leomil Coelho, Leonardo Augusto de Almeida
Brazilian Journal of Microbiology, 2025
A computational approach for MHC-restricted multi-epitope vaccine design targeting Oropouche virus structural proteins
Letícia Barbosa Silva, Laura Leone da Silva, Leonardo Pereira de Araújo, Evandro Neves Silva, Patrícia Paiva Corsetti, Leonardo Augusto de Almeida
Acta Tropica, 2025
Identification of cis-sQTL demonstrates genetic associations and functional implications of inflammatory processes in Nelore cattle muscle tissue
Thaís Cristina Ferreira Dos Santos, Evandro Neves Silva, Gabriela Bonfá Frezarim, Bruna Maria Salatta, Fernando Baldi, Larissa Fernanda Simielli Fonseca, Lucia Galvão De Albuquerque, Maria Malane Magalhães Muniz, Danielly Beraldo Dos Santos Silva
Mammalian Genome, 2025
Cis-eQTL analysis reveals genes involved in biological processes of the immune system in Nelore cattle
Thaís Cristina Ferreira Dos Santos, Evandro Neves Silva, Gabriela Bonfá Frezarim, Bruna Maria Salatta, Fernando Baldi, Larissa Fernanda Simielli Fonseca, Lucia Galvão De Albuquerque, Maria Malane Magalhães Muniz, Danielly Beraldo Dos Santos Silva
Gene, 2025
Amoxicillin-induced bacterial gut dysbiosis decreases IL-1β and IL-6 expression but exacerbate lung inflammatory response against Mycobacterium bovis—Bacille Calmette-Guérin (BCG)
Tatimara M. Miyauchi-Tavares, Evandro Neves Silva, Joyce Alves dos Santos, Priscila V. Sousa, Marcos F. Teodoro Braga, Caroline M. Carminatti, Victoria B. Lanza, Bruna C. Fagundes, Rômulo Dias Novaes, Leonardo Augusto de Almeida, Patrícia Paiva Corsetti
Plos One, 2025
What is the Best Option for Cellular Immunotherapies in Triple-Negative Breast Cancer? A Systematic Review Based on in vitro and in vivo Evidences
Joyce Alves dos Santos, Giovanna Porfírio Rosa, Yasmin Vieira Braida, Evandro Neves Silva, Leonardo Augusto de Almeida, Patrícia Paiva Corsetti
Brazilian Archives of Biology and Technology, 2025
Shared immunogenic epitopes between host entry and exit proteins from monkeypox and Alaskapox viruses
Leonardo Pereira de Araújo, Evandro Neves Silva, Patrícia Paiva Corsetti, Leonardo Augusto de Almeida
Lancet Microbe, 2024
A network-based approach to understanding gene–biological processes affecting economically important traits of Nelore cattle
Mariely Simone Lopes Corrêa, Evandro Neves Silva, Thaís Cristina Ferreira dos Santos, Larissa Fernanda Simielli Fonseca, Ana Fabrícia Braga Magalhães, Lucas Lima Verardo, Lucia Galvão de Albuquerque, Danielly Beraldo dos Santos Silva
Animal Genetics, 2024
Reactive oxygen species trigger inflammasome activation after intracellular microbial interaction
Caio Pupin Rosa, Thiago Caetano Andrade Belo, Natália Cristina de Melo Santos, Evandro Neves Silva, Juciano Gasparotto, Patrícia Paiva Corsetti, Leonardo Augusto de Almeida
Life Sciences, 2023
Cis-regulatory modules prediction in spliced genes associated with carcass and meat traits of Nelore cattle
Thaís Cristina Ferreira Dos Santos, Evandro Neves Silva, Larissa Fernanda Simielli Fonseca, Lucia Galvão De Albuquerque, Danielly Beraldo Dos Santos Silva
Animal Gene, 2023
Different drug approaches to COVID-19 treatment worldwide: an update of new drugs and drugs repositioning to fight against the novel coronavirus
Josidel Conceição Oliver, Evandro Neves Silva, Letícia Martins Soares, Gislaine Cristina Scodeler, Ana de Souza Santos, Patrícia Paiva Corsetti, Carlos Roberto Prudêncio, Leonardo Augusto de Almeida
Therapeutic Advances in Vaccines and Immunotherapy, 2022
Amoxicillin-induced gut dysbiosis influences estrous cycle in mice and cytokine expression in the ovary and the caecum
Evandro Neves Silva, Thaís Viana Fialho Martins, Tatimara Maria Miyauchi‐Tavares, Bianca Aparecida Expedito Miranda, Gabriela de Assis Santos, Caio Pupin Rosa, Jeferson Antônio Santos, Rômulo Dias Novaes, Leonardo Augusto Almeida, Patrícia Paiva Corsetti
American Journal of Reproductive Immunology, 2020
Vancomycin-induced gut dysbiosis during Pseudomonas aeruginosa pulmonary infection in a mice model
Caio Pupin Rosa, Jéssica Assis Pereira, Natália Cristina de Melo Santos, Gustavo Andrade Brancaglion, Evandro Neves Silva, Carlos Alberto Tagliati, Rômulo Dias Novaes, Patrícia Paiva Corsetti, Leonardo Augusto de Almeida
Journal of Leukocyte Biology, 2020

Publications

1. Cis-regulatory modules prediction in spliced genes associated with carcass and meat traits of Nelore cattle. Animal Gene, v. 27, p. 200142, 2023.
2. Esporotricose felina: Conduta clínica, diagnóstico e tratamento preconizado no município de Vitória - ES. RESEARCH, SOCIETY AND DEVELOPMENT, v. 11, p. e589111031028, 2022.
3. Different drug approaches to COVID-19 treatment worldwide: an update of new drugs and drugs repositioning to fight against the novel coronavirus. Therapeutic Advances in Vaccines and Immunotherapy, v. 10, p. 251513552211448, 2022.
4. Incidence of Staphylococcus aureus on equipment from a gym of physical activities in Alfenas - MG. RESEARCH, SOCIETY AND DEVELOPMENT, v. 10, p. e15101220056, 2021.
5. Vancomycin-induced gut dysbiosis during Pseudomonas aeruginosa pulmonary infection in a mice model. JOURNAL OF LEUKOCYTE BIOLOGY, v. 107, p. 95-104, 2020.
6. Caracterização bromatológica da sementes de Aleurites moluccana (L.) Willd.. REVISTA BRASILEIRA DE OBESIDADE, NUTRIÇÃO E EMAGRECIMENTO, v. 14, p. 29-36, 2020.
7. Amoxicillin-induced gut dysbiosis influences estrous cycle in mice and cytokine expression in the ovary and the caecum. AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY, v. 84, p. e13247, 2020.
8. Avaliação dos parâmetros nutricionais de refeições transportadas em um restaurante comercial na cidade de Alfenas - MG. REVISTA BRASILEIRA DE OBESIDADE, NUTRIÇÃO E EMAGRECIMENTO, v. 13, p. 1168-1174, 2019.

GRANT DETAILS

1. Society Brazilian of Immunology (SBI).
2. Society for Leukocyte Biology (SLB).
3. Center for Assisted Human Reproduction (NERHA).

Industry, Institute, or Organisation Collaboration

1. Researcher/Master's student member. Laboratory of Immunology of Infectious and Chronic Diseases (LIDIC). José do Rosário Vellano University (UNIFENAS).
2. Member researcher/collaborator. Laboratory of Molecular Biology of Microorganisms (LaBioMol). Federal University of Alfenas (UNIFAL).
3. Researcher/doctoral member. Laboratory of Molecular and Cellular Biology (BioMol). José do Rosário Vellano University (UNIFENAS).
4. Member of the Center for Academic Studies in Human Reproduction. José do Rosário Vellano University (UNIFENAS).

INDUSTRY EXPERIENCE

1. Laboratory of Molecular and Cellular Biology (BioMol)/ UNIFENAS.
2. Laboratory of Molecular Biology of Microorganisms (LaBioMol)/ UNIFAL.

SOCIAL, ECONOMIC, or ACADEMIC BENEFITS

1. Integral member, treasurer, vice-president and president of Academic League. José do Rosário Vellano University (UNIFENAS), Biomedicine Course.