Josiane Erica Miyamoto

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PhD student Laboratory of Metabolic Disorders/Schoo of Applied Sciences - University of Campinas

Josiane Erica Miyamoto


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https://researchid.co/josimiyamoto
13

Scopus Publications

Scopus Publications

  • DICER-driven regulation of lipid metabolism and macrophage-adipocyte crosstalk in partial lipodystrophy
    Andréa L. Rocha, Rhaissa Godoi, Tanes Imamura de Lima, Diogo de Moraes, Bruna Brasil Brandão, Antonio F.M. Pinto, Heloísa Antoniella Braz-de-Melo, Felipe Corrêa da Silva, Tiago Tomazini Gonçalves, Letícia de Souza Figueiredo, João Pedro Mazzi, Niedson Correia de Lima Junior, Safira Maria Leite Sousa, Josiane Erica Miyamoto, Luiz Osório Leiria, Jan-Wilhelm Kornfeld, Kelly Grace Magalhães, Pedro Manoel Moraes-Vieira, Alan Saghatelian, Marcelo A. Mori
    Biomedicine and Pharmacotherapy, 2026
    Partial lipodystrophy is characterized by abnormal fat distribution, impaired thermogenesis, systemic inflammation, and diminished DICER1 expression in adipose tissue. Using adipocyte-specific Dicer1-deficient murine models to mimic lipodystrophy, we identified early metabolic and inflammatory alterations associated with the onset of partial lipodystrophy. DICER deficiency in adipocytes impaired lipid metabolism, leading to increased release of saturated lipids and triglycerides into the medium while reducing the biosynthesis of anti-inflammatory lipids. These changes promoted a pro-inflammatory environment by inducing macrophages to release IL-1β. In parallel, both adipocyte-autonomous DICER deficiency and the resulting inflammatory environment impaired adipocyte thermogenic capacity. Lipidomic analyses revealed reduced levels of docosahexaenoic acid (DHA) and other polyunsaturated fatty acids (PUFAs) in Dicer1-deficient adipocytes, accompanied by increased expression of genes encoding CYP450 enzymes linked to lipid degradation, and reduced DHA biosynthesis. Co-culture experiments confirmed that Dicer1-deficient adipocytes exacerbate macrophage-driven pro-inflammatory profile and thermogenic gene suppression. Notably, DHA supplementation mitigated the pro-inflammatory response triggered by conditioned media from Dicer1-deficient adipocytes. These findings reveal a critical role for adipocyte DICER in regulating lipid metabolism, macrophage-adipocyte interactions, and thermogenic function, offering new insights into molecular mechanisms driving metabolic dysfunction and chronic inflammation associated with partial lipodystrophy.
  • Evaluation of absorption kinetics and bioavailability of palmitic acid and 2-palmitoylglycerol, a metabolite of interesterified fat, radiolabeled with99m-technetium
    Penélope Lacrísio dos Reis Menta, Maria Emília Rabelo Andrade, Amanda Dias Borges, Luiz Octávio Pires, Josiane Érica Miyamoto, Beatriz Piatezzi Siqueira, Letícia Martins Ignacio-Souza, Adriana Souza Torsoni, Marcio Alberto Torsoni, Simone Odilia Antunes Fernandes, Valbert Nascimento Cardoso, Marciane Milanski
    Journal of Nutritional Biochemistry, 2026
  • Interesterified palm oil leads to distinct variation in the intestinal microbiota of mice
    Penélope Lacrísio dos Reis Menta, Alexander Umanets, Josiane Erica Miyamoto, Beatriz Piatezzi Siqueira, Adilson Sartoratto, Letícia Ignácio-Souza, Adriana Souza Torsoni, Marcio Alberto Torsoni, Raquel Franco Leal, Adriane Elisabete Costa Antunes, Marciane Milanski
    Food Bioscience, 2024
  • Interesterified palm oil promotes insulin resistance and altered insulin secretion and signaling in Swiss mice
    Raísa Magno dos Santos, Josiane Érica Miyamoto, Beatriz Piatezzi Siqueira, Thiago Reis Araujo, Jean Franciesco Vettorazzi, Penelope Lacrisio Reis Menta, Jessica Denom, Márcia Queiroz Latorraca, Céline Cruciani-Guglielmacci, Everardo Magalhães Carneiro, Adriana Torsoni, Marcio Torsoni, Ana Paula Badan, Christophe Magnan, Hervé Le Stunff, Letícia Ignácio-Souza, Marciane Milanski
    Food Research International, 2024
  • Obesity phenotype induced by high-fat diet leads to maternal-fetal constraint, placental inefficiency, and fetal growth restriction in mice
    Ana Paula Varela Sanches, Josilene Lopes de Oliveira, Maíra Schuchter Ferreira, Bruna de Souza Lima, Josiane Érica Miyamoto, Laís Angélica de Paula Simino, Márcio Alberto Torsoni, Adriana Souza Torsoni, Marciane Milanski, Letícia Ignácio-Souza
    Journal of Nutritional Biochemistry, 2022
  • Interesterified palm oil increases intestinal permeability, promotes bacterial translocation, alters inflammatory parameters and tight-junction protein genic expression in Swiss mice
    Penélope Lacrísio Reis Menta, Maria Emília Rabelo Andrade, Lívia Furquim de Castro, Luísa Martins Trindade, Melissa Tainan Silva Dias, Josiane Érica Miyamoto, Raisa Magno dos Santos, Geovanni Dantas Cassali, Raquel Franco Leal, Ana Paula Badan Ribeiro, Renato Grimaldi, Letícia Martins Ignacio-Souza, Marcio Alberto Torsoni, Adriana Souza Torsoni, Valbert Nascimento Cardoso, Marciane Milanski
    Food Research International, 2022
  • Beet (Beta vulgaris L.) stalk and leaf supplementation changes the glucose homeostasis and inflammatory markers in the liver of mice exposed to a high-fat diet
    Isabela Micheletti Lorizola, Josiane Érica Miyamoto, Ana Luiza Figueiredo Vieira, Beatriz Rocchetti Sumere, Rosângela Maria Neves Bezerra, Marcio Alberto Torsoni, Adriana Souza Torsoni, Mauricio Ariel Rostagno, Marciane Milanski, Caroline Dário Capitani
    Food Chemistry Molecular Sciences, 2021
    Although beet stalks and leaves are not consumed and are usually discarded, they are an important source of bioactive flavonoids possessing antioxidant and anti-inflammatory activity. The aim of this study was to assess the effect of supplementation with beet stalks and leaves on metabolic parameters and glucose homeostasis in mice exposed to a high-fat diet. Six-week-old male Swiss mice were randomly divided into five experimental groups submitted to either standard diet (CT) or high-fat diet (HF), and HF-fed mice were subdivided into three treatment groups supplemented with oven-dehydrated beet stalks and leaves (SL), lyophilized beet stalks and leaves (Ly) or beet stalk and leaf extract (EX). Supplementation with SL promoted a mild improvement in the glucose homeostasis and decreased the protein levels of TNFα with no alterations in hepatic triglyceride content. It remains to be clarified if the enhancement in the glucose homeostasis observed in HFSL could be a consequence of improvement in pancreatic insulin secretion and/or glucose uptake from skeletal muscle and white adipose tissues.
  • Dietary Patterns Associated to Clinical Aspects in Crohn’s Disease Patients
    Marina Moreira de Castro, Ligiana Pires Corona, Lívia Bitencourt Pascoal, Josiane Érica Miyamoto, Leticia Martins Ignacio-Souza, Maria de Lourdes Setsuko Ayrizono, Marcio Alberto Torsoni, Adriana Souza Torsoni, Raquel Franco Leal, Marciane Milanski
    Scientific Reports, 2020
    Diet is an important factor in both the pathogenesis and in the clinical course of Crohn’s disease (CD). However, data on dietary patterns of CD patients are rather limited in the literature. This cross-sectional study included 60 patients with CD, aged 18–60 years. Dietary intake was assessed using a validated food frequency questionnaire to measure food consumption patterns by principal component analysis (PCA). Multiple regression analysis was performed to investigate the association between dietary patterns and clinical and demographic variables. Three dietary patterns were identified: “Traditional + FODMAP” was associated with symptoms, gender, previous surgeries, and duration of the disease. “Fitness style” was positively associated with physical activity and negatively associated with body mass index and smoking. “Snacks and processed foods” was positively associated with duration of the disease and negatively associated with age. According to the weekly food consumption analysis, patients with active disease consumed less coffee and tea. We found significant associations between the three dietary patterns and the variables, but not with the stage of the disease. Prospective studies are necessary to determine the effects of food consumption patterns on the clinical course of CD.
  • Interesterified palm oil impairs glucose homeostasis and induces deleterious effects in liver of Swiss mice
    Josiane Érica Miyamoto, Andressa Reginato, Mariana Portovedo, Raísa Magno dos Santos, Marcella Aparecida Stahl, Hervé Le Stunff, Márcia Queiroz Latorraca, Marise Auxiliadora de Barros Reis, Vanessa Cristina Arantes, Diego Luiz Doneda, Leticia Martins Ignacio-Souza, Adriana Souza Torsoni, Renato Grimaldi, Ana Paula Badan Ribeiro, Marcio Alberto Torsoni, Marciane Milanski
    Metabolism Clinical and Experimental, 2020
  • Acute effects of fatty acids on autophagy in NPY neurones
    Andressa Reginato, Beatriz Piatezzi Siqueira, Josiane Érica Miyamoto, Mariana Portovedo, Suleyma de Oliveira Costa, Thaís Fante, Hosana Gomes Rodrigues, Letícia Martins Ignácio‐Souza, Márcio Alberto Torsoni, Adriana Souza Torsoni, Hervé Le Stunff, Denise D. Belsham, Marciane Milanski
    Journal of Neuroendocrinology, 2020
    High‐fat diet (HFD) feeding is deleterious to hypothalamic tissue, leading to inflammation and lipotoxicity, as well as contributing to central insulin resistance. Autophagy is a process that restores cellular homeostasis by degrading malfunctioning organelles and proteins. Chronic HFD‐feeding down‐regulates hypothalamic autophagy. However, the effects of short‐term HFD‐feeding and the saturated fatty acid palmitate (PA) on hypothalamic autophagy and in neurones that express neuropeptide Y (NPY) and agouti‐related peptide remains unknown. Therefore, we assessed hypothalamic autophagy after 1 and 3 days of HFD‐feeding. We also injected PA i.c.v and analysed the modulation of autophagy in hypothalamic tissue. Both interventions resulted in changes in autophagy‐related gene profiles without significant differences in protein content of p62 and LC3B‐II, markers of the autophagy pathway. When we assessed native NPY neurones in brain slices from PA‐treated animals, we observed increased levels of Atg7 and LC3B protein in response to PA treatment, indicating the induction of autophagy. We then tested the direct effects of fatty acids using the immortalised hypothalamic NPY‐expressing neuronal cell model mHypoE‐46. We found that PA, but not palmitoleate (PO) (a monounsaturated fatty acid), was able to induce autophagy. Co‐treatment with PA and PO was able to block the PA‐mediated induction of autophagy, as assessed by flow cytometry. When the de novo ceramide synthesis pathway was blocked with myriocin pre‐treatment, we observed a decrease in PA‐mediated induction of autophagy, although there was no change with the toll‐like receptor 4 inhibitor, TAK‐242. Taken together, these findings provide evidence that saturated and unsaturated fatty acids can differentially regulate hypothalamic autophagy and that ceramide synthesis may be an important mediator of those effects. Understanding the mechanisms by which dietary fats affect autophagy in neurones involved in the control of energy homeostasis will provide potential new pathways for targeting and containing the obesity epidemic.
  • Lipid excess affects chaperone-mediated autophagy in hypothalamus
    M. Portovedo, A. Reginato, J.É. Miyamoto, L.A. Simino, M.P. Hakim, M. Campana, R.F. Leal, L.M. Ignácio-Souza, M.A. Torsoni, C. Magnan, H. Le Stunff, A.S. Torsoni, M. Milanski
    Biochimie, 2020
  • Interesterified soybean oil promotes weight gain, impaired glucose tolerance and increased liver cellular stress markers
    Josiane Érica Miyamoto, Ana Carolina G. Ferraz, Mariana Portovedo, Andressa Reginato, Marcella Aparecida Stahl, Leticia Martins Ignacio-Souza, Kenny L. Chan, Adriana Souza Torsoni, Marcio Alberto Torsoni, Ana Paula Badan Ribeiro, Marciane Milanski
    Journal of Nutritional Biochemistry, 2018
  • Autophagy proteins are modulated in the liver and hypothalamus of the offspring of mice with diet-induced obesity
    Andressa Reginato, Thaís de Fante, Mariana Portovedo, Natália Ferreira da Costa, Tanyara Baliani Payolla, Josiane Érica Miyamotto, Laís Angélica Simino, Letícia M. Ignácio-Souza, Márcio A. Torsoni, Adriana S. Torsoni, Marciane Milanski
    Journal of Nutritional Biochemistry, 2016