Dr. Rajesh Kanna Gopal
@saveethadental.com
Assistant Professor, Department of Microbiology,
Saveetha Dental College and Hospital, Saveetha Institute of Medical and Technical Sciences (SIMATS), Chennai - 600077
I have eight years of research experience in the field of Algal Biotechnology (Marine macroalgae, isolation and culturing microalgae). At present, I am working on antiviral and anti-arthritic potential of bioactive compounds, industrial and domestic wastewater Phycoremediation, alcoholic beverages, and bioplastic from macroalgae (Seaweed); Non-ribosomal polypeptides and Exopolysaccharides from cyanobacteria, LDPE degradation by microalgae.
EDUCATION
• PhD: Plant Biology and Plant Biotechnology, Thesis entitled: Isolation, Purification and Characterization of Superoxide dismutase (SOD) enzyme from Microalgae – 2014-2019, University of Madras, Chennai, Tamil Nadu, India.
• M.Phil.: Botany, Dissertation entitled: Studies on the Detection, Production and Analysis of Cholesterol Oxidase from Stenotrophomonas sp. – 2011-2012, GPA: 7.8, University of Madras, Chennai, Tamil Nadu, India.
• M.Sc.: Plant Biology and Plant Biotechnology – 2009-2011, GPA: 8.19, University of Madras, Chennai, Tamil Nadu, India.
• B.Sc.: Plant Biology and Plant Biotechnology – 2006-2009, GPA: 7.2 University of Madras, Chennai, Tamil Nadu, India.
RESEARCH, TEACHING, or OTHER INTERESTS
Biotechnology, Medicine, Dentistry
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
Elumalai Sanniyasi, Rajesh Kanna Gopal, Preethy P. Raj, Kalaiselvi R., and Chandhinipriya S.
Elsevier BV
Elumalai Sanniyasi, Rajesh Kanna Gopal, Rajesh Damodharan, Tarani Thirumurugan, and Vishali Mahendran
Springer Science and Business Media LLC
Elumalai Sanniyasi, Rajesh Kanna Gopal, Rajesh Damodharan, Arthi Arumugam, Madhumitha Sampath Kumar, Nandhini Senthilkumar, and Monisha Anbalagan
Springer Science and Business Media LLC
AbstractMarine seaweeds are rich source of polysaccharides present in their cell wall and are cultivated and consumed in China, Japan, Korea, and South Asian countries. Brown seaweeds (Phaeophyta) are rich source of polysaccharides such as Laminarin and Fucoidan. In present study, both the laminarin and fucoidan were isolated was yielded higher in PP (Padina pavonica) (4.36%) and STM (Stoechospermum marginatum) (2.32%), respectively. The carbohydrate content in laminarin and fucoidan was 86.91% and 87.36%, whereas the sulphate content in fucoidan was 20.68%. Glucose and mannose were the major monosaccharide units in laminarin (PP), however, fucose, galactose, and xylose in fucoidan (STM). FT-IR down peaks represent the carbohydrate of laminarin and fucoidan except, for 1219 cm−1, and 843 cm−1, illustrating the sulphate groups of fucoidan. The molecular weight of laminarin was 3–5 kDa, and the same for fucoidan was 2–6 kDa, respectively. Both the Fucoidan and Laminarin showed null cytotoxicity on Vero cells. Contrastingly, the fucoidan possess cytotoxic activity on human liver cancer cells (HepG2) (IC50—24.4 ± 1.5 µg/mL). Simultaneously, laminarin also shown cytotoxicity on human colon cancer cells (HT-29) (IC50—57 ± 1.2 µg/mL). The AO/EB (Acriding Orange/Ethidium Bromide) assay significantly resulted in apoptosis and necrosis upon laminarin and fucoidan treatments, respectively. The DNA fragmentation results support necrotic cancer cell death. Therefore, laminarin and fucoidan from PP and STM were potential bioactive compounds for anticancer therapy.
Elumalai Sanniyasi, Rajesh Kanna Gopal, Preethy P. Raj, and Ashok Kumar Shanmugavel
Elsevier BV
Elumalai Sanniyasi, Antony Prakash Rejoy Patrick, Kreedika Rajagopalan, Rajesh Kanna Gopal, and Rajesh Damodharan
Springer Science and Business Media LLC
AbstractDiatoms are photoautotrophic microalgae classified under class Bacillariophyceae, engulfed by hard silicate frustules, which give mechanical support and protection from bacterial infections. They exude polysaccharides extracellularly that help them with their gliding motion (locomotion). However, the bioactivity of such compounds was least explored from freshwater diatoms. In the present study, a single species of pennate diatom identified as Nitzschia palea was isolated and molecularly characterized by 18S rRNA smaller subunit gene (partial) sequencing and submitted to GenBank NCBI and accession number retrieved as ON360983. Based on logarithmic growth curve analysis, the exponential phase was obtained from 3rd to 4th day of diatom culture. The exopolysaccharide was extracted by the hot-water extraction method, and characterized by FT-IR. The total yield of exopolysaccharide from Nitzschia palea was estimated as 1.56 mg in 100 mL of culture after 7 days of incubation. The estimated carbohydrate content was 51.35 µg/100 µL. The monosaccharide constituents were determined by acid hydrolysis of exopolysaccharide, silylation (derivatization), followed by GC–MS analysis and tabulated. The extracted exopolysaccharide was evaluated for its anti-cancer potential against the Human Adenocarcinoma lung cancer cell line (A549) and the estimated IC50 value was 62.64 µg/mL. Acridine orange staining assay and DNA fragmentation assay also confirmed the apoptotic activity of exopolysaccharide derived from the diatom Nitzschia palea.
Elumalai Sanniyasi, Rajesh Kanna Gopal, Dinesh Kumar Gunasekar, and Preethy P. Raj
Springer Science and Business Media LLC
AbstractPlastic (polyethylene) pollution is a severe cause of deterioration of a healthy environment. For example, ingestion of plastics in the animal gut, clogging of water canals and retarded solid waste management. Many conventional methods of polyethylene degradation include UV photooxidation, thermal oxidation, incineration, chemical oxidation and landfill are being practiced. However, these methods are not feasible, costlier and not a complete solution for this global issue. Therefore, plausible, alternative solution for this issue is biodegradation. Microbes such as bacteria, fungi and algae are involved in polyethylene degradation in its natural habitat. Among them, algae were given very less importance. In our present study, a potential microalga, morphologically identified as Uronema africanum Borge, isolated from a waste plastic bag collected from a domestic waste dumping site in a freshwater lake. This microalga was further treated with the LDPE sheet in BBM culture medium. Based on the results obtained from light microscopy, dark field microscopy, GC–MS, FT-IR, SEM and AFM, it was concluded that the microalga has initiated degradation of LDPE sheet within 30 days of incubation. Concurrently, the configuration of corrosions, abrasions, grooves and ridges were found similar with the morphological features of the microalga. For example, the configuration of the radial disc-like attachment structure of the microalga was found corresponding to the abrasions on the surface of LDPE sheet at an average size of 20–30 µm in diameter. Whereas, the configuration of ridges and grooves were found similar with the filamentous nature of the microalga (10–15 µm width). This is a hitherto report on the biodegradation of LDPE sheet by the microalga Uronema africanum Borge.
A. Yoganandhan, G. Rajesh Kanna, S.D. Subhash, and J. Hebinson Jothi
Elsevier BV
Elumalai Sanniyasi, Gayathri Venkatasubramanian, Madhu Mitra Anbalagan, Preethy P. Raj, and Rajesh Kanna Gopal
Springer Science and Business Media LLC
AbstractHighly active antiretroviral therapy (HAART) is the only available remedial measure to treat HIV infected patients, as recognized by the WHO. However, it is associated with toxicity (nephrotoxicity), high cost and most preferably drug resistance in the first-line treatment. Wherefore, potential and novel natural source is the only option for the modern world to challenge this global issue. In recent years, sulfated polysaccharide from marine macroalgae shown to be biologically active as anti-inflammatory, anticoagulant, antitumor, immunomodulatory and antiviral agents. As a direct inhibitor of HIV including other retroviruses, it is considered as a “new generation antiretroviral drug”. In our present study, Fucoidan, a sulfated polysaccharide has been extracted from two different macroalgae Dictyota bartayesiana (DD) and Turbinaria decurrens (TD) based on hot water extraction method and further confirmed by FT-IR and RP-HPLC methods. Both the crude and purified fucoidan samples were evaluated for anti-HIV activity after ion exchange chromatography purification. The maximum inhibitory activity of crude and purified fucoidan samples are 90.5% and 89% in the fucoidan extracts of DD. Whereas, it was 89.7% and 92% in the fucoidan extracts of TD. Simultaneously, the IC50 values were determined and recorded as 1.56 µg/ml and 57.6 ng/ml in both the crude and purified fucoidan extracts of DD respectively. Similarly, for TD, it was 3 µg/ml and 131.7 ng/ml in the fucoidan extracts of TD. Therefore, further extensive research work is the most needful to fill the gaps to develop this sulfated polysaccharide as a potential drug for the treatment of HIV patients.
RECENT SCHOLAR PUBLICATIONS
MOST CITED SCHOLAR PUBLICATIONS
RESEARCH OUTPUTS (PATENTS, SOFTWARE, PUBLICATIONS, PRODUCTS)
1. Title of the Invention: Brewing of Alcoholic Beverage (Beer) from a Marine Seaweed.
Patent Application Number: 202241036660, Status: Applied
2. Title of the Invention: Blue-green tea: the future of beverages.
Status: Filing under process