Jyoti Joshi
@ggdsd.ac.in
Scopus Publications
Scopus Publications
Nimisha Jain, Angelina Mary, Tanu Singh, Srushti Gadiyaram, Jyoti Joshi, D. Amilan Jose, and Abbas Raja Naziruddin
Wiley
AbstractWe report heteroleptic ruthenium complexes of terpyridine (tpy) ligands with directly linked carboxylic acid anchors. These complexes feature methyl or methoxy‐substituted 4′−Phtpy as donor ligands. We prepared these heteroleptic complexes from the ruthenium (II) precursor via a milder route to preclude the homoleptic complex formation. The donor−acceptor arrangement of tpy ligands in these ruthenium complexes renders visible light absorption giving metal and ligand‐to‐ligand charge transfer excitations at c.a. 490 nm. We evaluate the effect of the tpy donor substituents on the light‐harvesting ability in Dye‐Sensitized Solar Cells (DSSCs) and compare their photosensitizing ability with heteroleptic complexes bearing phenyl spacer at the acceptor end. Further, scrutinizing their photovoltaic performance, we studied their electron transfer kinetics in DSSCs using electrochemical impedance spectroscopy. This paper presents the structure‐photosensitization relationship of these heteroleptic ruthenium complexes through a combined experimental and computational approach.
Nawal Kishore Sahu, Ritu Sharma, Kshirsagar Prasad Suhas, Jyoti Joshi, Kunal Prakash, Richa Sharma, Ramendra Pratap, Xiwen Hu, Sukhbir Kaur, Mukesh Jain,et al.
MDPI AG
Leishmaniasis is a neglected tropical disease, and there is an emerging need for the development of effective drugs to treat it. To identify novel compounds with antileishmanial properties, a novel series of functionalized spiro[indoline-3,2′-pyrrolidin]-2-one/spiro[indoline-3,3′-pyrrolizin]-2-one 23a–f, 24a–f, and 25a–g were prepared from natural-product-inspired pharmaceutically privileged bioactive sub-structures, i.e., isatins 20a–h, various substituted chalcones 21a–f, and 22a–c amino acids, via 1,3-dipolar cycloaddition reactions in MeOH at 80 °C using a microwave-assisted approach. Compared to traditional methods, microwave-assisted synthesis produces higher yields and better quality, and it takes less time. We report here the in vitro antileishmanial activity against Leishmania donovani and SAR studies. The analogues 24a, 24e, 24f, and 25d were found to be the most active compounds of the series and showed IC50 values of 2.43 µM, 0.96 µM, 1.62 µM, and 3.55 µM, respectively, compared to the standard reference drug Amphotericin B (IC50 = 0.060 µM). All compounds were assessed for Leishmania DNA topoisomerase type IB inhibition activity using the standard drug Camptothecin, and 24a, 24e, 24f, and 25d showed potential results. In order to further validate the experimental results and gain a deeper understanding of the binding manner of such compounds, molecular docking studies were also performed. The stereochemistry of the novel functionalized spirooxindole derivatives was confirmed by single-crystal X-ray crystallography studies.
Harshit Patel and Jyoti Joshi
Springer Science and Business Media LLC
Tanuja Kumari, Ramhari Meena, Lopamudra Giri, Bipul Sarma, Priyanka R. Angarkhe, Jesni M. Jacob, Jagnyaseni Tripathy, Jyoti Joshi, Mahesh Kumar Ravva, Rakesh K. Behera,et al.
Elsevier BV
Jyoti Joshi, Rupinder Kaur, and Sukhbir Kaur
CRC Press
Isha Saini, Jyoti Joshi, and Sukhbir Kaur
Elsevier BV
Richa Sharma, Ravi Kant Yadav, Mukesh Jain, Jyoti Joshi, and Sandeep Chaudhary
American Chemical Society (ACS)
A highly efficient oxidant-switched palladium-catalyzed regioselective C(sp2)-H/C(sp2)-H cross-dehydrogenative coupling (CDC) for direct mono/bis-ortho-aroylation of substituted 1-phenyl-1H-indazoles 1a-j with various substituted aldehydes 3a-t via C(sp2)-H bond activation has been developed. In this study, Pd-catalyzed chelation-assisted mono- or bis-aroylation of substituted 1-phenyl-1H-indazoles depends on the type of oxidant being used for the CDC reaction. While mono-ortho-aroylation of substituted 1-phenyl-1H-indazole was obtained using dicumylperoxide (DCP) as the oxidant, the bis-ortho-aroylation product has been afforded by the use of tert-butyl hydroperoxide (TBHP). Regardless of the greater activity at the C-3 position of 1H-indazoles, the greater coordinating capacity of the N atom directed the aroylating group to the ortho position, leaving behind the nondirected metalation pathway. The Pd-catalyzed operationally simplified methodology proceeded in the presence of oxidants with either DCP or TBHP in dichloroethane as the solvent at 110 °C for 16 h, which generated a miscellaneous variety of monosubstituted o-benzoyl/acyl-1-aryl-1H-indazoles 4a-t/5a-i and bis-substituted o-benzoyl-1-aryl-1H-indazoles 6a-j in ≤88% yields. The probable mechanistic pathway involves a free radical chelation-assisted approach that could be accomplished by the addition of an in situ-generated oxidant-promoted benzoyl/acyl radical to the ortho position of 1-phenyl-1H-indazoles. A wide range of substrates, a high functional group tolerance, gram-scale synthesis, control/competitive experiments, and a variety of synthetic applications further exemplify the versatility of the developed methodology.
Jyoti Joshi, Chetna Bandral, Raj Kumar Manchanda, Anil Khurana, Debadatta Nayak, and Sukhbir Kaur
Georg Thieme Verlag KG
Abstract Background Visceral leishmaniasis (VL) is a neglected tropical disease that is fatal if treatment is not given. The available chemotherapeutic options are unsatisfactory, and so complementary therapies like homeopathy might be a promising approach. Methods A nosode from a pure axenic culture of Leishmania donovani was prepared and screened for its anti-leishmanial potential both in an in-vitro and an in-vivo experimental approach. Results Leishmania donovani amastigote promastigote nosode (LdAPN 30C) exhibited significant anti-leishmanial activity against the promastigote forms of Leishmania donovani and was found to be safe. A study conducted on VL-infected mice revealed that LdAPN 30C resolved the disease by modulating the host immune response toward the Th1 type through upregulating the pro-inflammatory cytokines (IFN-γ and IL-17) and inducing nitric oxide (NO) levels in the infected macrophages. The hepatic parasite load was also found to be significantly decreased. The nosode was found to be safe, as no histological alterations in the liver or kidney were observed in the animals treated with the LdAPN 30C. Conclusion This is the first study in which an axenic culture of Leishmania donovani has been used for the preparation of a homeopathic medication. The study highlights the anti-leishmanial and immunomodulatory potential of a homeopathic nosode in experimental VL.
Kamaljit Grewal, Jyoti Joshi, Sonia Rathee, Sukhbir Kaur, Harminder Pal Singh, and Daizy R. Batish
Informa UK Limited
Abstract The present study evaluated cytotoxic, anti-inflammatory, and anti-leishmanial properties of essential oil (EO) from fresh foliage of Eucalyptus camaldulensis Dehnh., and its major constituent compounds− 1,8-cineole, β-pinene and α-pinene. The chromatographic analysis of the hydrodistilled EO using gas chromatography-mass spectrometry (GC/MS) revealed the presence of 25 compounds which constituted 99.45% of EO. The EO comprised of 36% monoterpenoid hydrocarbons and 22.3% oxygenated monoterpenes. The most abundant constituent was 1,8-cineole (17.1%) followed by β-pinene (16.5%). In addition, significant quantities of β-eudesmol (10.9%), limonene (9.5%), and α-pinene (7.9%) were observed. Cell cytotoxicity concentration was observed to be highest in 1,8-cineole (CC50 > 640 µg/mL), followed by α-pinene (CC50 > 320 µg/mL), β-pinene (CC50 > 160 µg/mL) and EO (CC50 > 20 µg/mL). Investigation of anti-inflammatory potential by carrageenan-induced rat paw edema model revealed maximum inhibition in α-pinene (85.6% at 500 mg/kg body wt. and 81% at 250 mg/kg body wt.) after 3 h, which was comparable to indomethacin (positive control). Among the EO and its components, β-pinene exhibited maximum anti-leishmanial potential with IC50 of 2.57 ± 0.15 mL. The study concluded that E. camaldulensis EO possesses significant anti-inflammatory and anti-leishmanial activity, thus suggesting the use of EO and its major constituents as a green drug that is a safer alternative to synthetic compounds.
Ravi Kant Yadav, Richa Sharma, Deepak Gautam, Jyoti Joshi, and Sandeep Chaudhary
Wiley
AbstractHerein, we report the identification of new and fast halogenating reagent system consisting of Lewis acid AlX3 [X=Cl, Br, I] as a halogen source in the presence of tert‐butyl hydroperoxide (TBHP) which has been utilized for the direct regioselective halogenation on various fused bi‐/tri‐cyclic hetero‐aromatic congeners 3 a–q, 7 a–d, 8 and 9 via −H bond functionalization. The operationally simple protocol is quite fast and does not require the external halogenation source at 110 °C in 20–60 minutes and furnished halogenated fused heterocycles 4 a–p, 5 a–k, 6 a–l, 10 a–i, 11 a, 12 a–b in up to 96% yields. The gram‐scale synthesis, wide substrate scope, functional group tolerance, control experiments and application to further derivatization/functionalization for C−C bond formation further highlights the versatility of the developed methodology as well as the compatibility of the new catalyst. The combination of Lewis acid (AlX3) as a halogen source and TBHP (oxidant) as a halogenating reagent system is the first account for the direct regioselective halogenation of several fused bi‐/tri‐cyclic hetero‐aromatic congeners via −H bond functionalization.
Jyoti Joshi, Chetna Bandral, Raj Kumar Manchanda, Anil Khurana, Debadatta Nayak, and Sukhbir Kaur
Georg Thieme Verlag KG
AbstractBackground Leishmaniasis is one of several neglected tropical diseases that warrant serious attention. A disease of socio-economically poor people, it demands safer and cheaper drugs that help to overcome the limitations faced by the existing anti-leishmanials. Complementary or traditional medicines might be a good option, with an added advantage that resistance may not develop against these drugs. Thus, the present investigation was performed to evaluate the anti-leishmanial efficacy of an ultra-diluted homeopathic medicine (Iodium 30c) in experimental visceral leishmaniasis (VL).Methods Compliant with strict ethical standards in animal experimentation, the study was performed in-vivo in inbred BALB/c mice which were injected intravenously with 1 × 107 promastigotes of Leishmania donovani before (therapeutic) or after (prophylactic) treatment with Iodium 30c for 30 days. In other groups of mice (n = 6 per group), amphotericin B served as positive control, infected animals as the disease control, while the naïve controls included normal animals; animals receiving only Iodium 30c or Alcohol 30c served as sham controls. The anti-leishmanial efficacy was assessed by determining the hepatic parasite load and analysing percentages of CD4+ and CD8+ T cells. Biochemical analysis and histological studies were performed to check any toxicities.Results Iodium-treated animals showed a significantly reduced parasite load (to 1503 ± 39 Leishman Donovan Units, LDU) as compared with the infected controls (4489 ± 256 LDU) (p < 0.05): thus, the mean therapeutic efficacy of Iodium 30c was 66.5%. In addition, the population of CD4+ and CD8+ T cells was significantly increased (p < 0.05) after treatment. No toxicity was observed, as evidenced from biochemical and histopathological studies of the liver and kidneys. Efficacy of Iodium 30c prophylaxis was 58.3%, while the therapeutic efficacy of amphotericin B was 85.9%.Conclusion This original study has shown that Iodium 30c had significant impact in controlling parasite replication in experimental VL, though the effect was less than that using standard pharmaceutical treatment.
Manoj Kumar, Tanuja Kumari, Jyoti Joshi, Sunil Chhimpa, P.J. John, Raj Rajeshwar Malinda, and Bidya S. Joshi
Rasayan Journal of Chemistry
Triazine-based heterocycles constitute a class of pertinent heterocyclic compounds that are found ubiquitously in numerous pharmaceuticals, functional materials, and chiral catalysts or ligands. The synthesis and further physicochemical assessment of metal complexes viz. [Cu(ptt)2]2, [Ni(ptt)2]2.2H2O, [Zn(ptt)2].2H2O, [Cd(ptt)2].2H2O (PTTH: 5-Phenyl-1,2,4-triazine-3(2H)-thione) are delineated in this account. The prepared complexes were identified quantitatively and qualitatively by a variety of physicochemical techniques, namely elemental analysis, FT-IR, LC-MS and 1HNMR spectroscopy. Spectral data show that Cu and Ni complexes comprise a binuclear framework, in which the organic molecule simulates as a mono-functional bidentate SNdonor and with two of the four moieties of the molecule performing a bridge to coordinates the central metal ions with the sulphur atom while a mononuclear structure is obtained in the ZnII and CdII complexes. Subsequently, FT-IR and LC-MS spectra also confirmed the coordinated aqua molecules in the NiII, ZnII and CdII complexes. The ligand and its complexes exhibited considerable antimicrobial activities against the Gram-positive bacteria (Bacillus subtilis: MTCC-121) as well as Gram-negative bacteria (Escherichia coli: MTCC-40). In the present study, NiII complex showed the highest antibacterial activity with 33 mm diameter of zone of inhibition. Electrochemical reduction behavior of ligand (PTTH), CuII and NiII complexes was explored at glassy carbon electrode using cyclic voltammetry.
Shivani Thakur, Jyoti Joshi, and Sukhbir Kaur
Springer Science and Business Media LLC
Varun Gupta, Dinesh K. Dhull, Jyoti Joshi, Sukhbir Kaur, and Anil Kumar
Elsevier BV
Tanuja Kumari, Ram Gopal, Ankit Goyal, and Jyoti Joshi
Springer Science and Business Media LLC
Tanuja Kumari, Ram Gopal, Ankit Goyal, and Jyoti Joshi
Springer Science and Business Media LLC
Jyoti Joshi, Anshu Dandia, and Sukhbeer Kumari
Bentham Science Publishers Ltd.
Harpreet Kaur, Rajni Attri, and Jyoti Joshi
Elsevier BV
S. Kaur, T. Kaur and J. Joshi
Journal of Biomedical Research
Abstract The current study was designed to examine the protective efficacy of DNA vaccines based on gp63 and Hsp70 against murine visceral leishmaniasis. Inbred BALB/c mice were immunized subcutaneously twice at an interval of three weeks with pcDNA3.1(+) encoding T cell epitopes of gp63 and Hsp70 individually and in combination. Animals were challenged intracardially with 107 promastigotes of Leishmania donovani 10 days post immunization and sacrificed 1, 2 and 3 months post challenge. The immunized animals revealed a significant reduction (P < 0.05) in splenic and hepatic parasite burden as compared to the infected controls. Maximum reduction in parasite load (P < 0.05) was observed in animals treated with a combination of pcDNA/gp63 and pcDNA/Hsp70. These animals also showed heightened DTH response, increased IgG2a, elevated Th1 cytokines (IFN-γ and IL-2) and reduced IgG1 and IL-10 levels. Thus, mice immunized with the cocktail vaccine exhibited significantly greater protection in comparison to those immunized with individual antigens.
Rajeev Nagill, Tejinder Kaur, Jyoti Joshi, and Sukhbir Kaur
Medknow
Anshu Dandia, Ruby Singh, Jyoti Joshi, and Shuchi Maheshwari
Springer Science and Business Media LLC
Naveen Gautam, Ankita Garg, Thandi Lal, Dinesh Chand Gautam, and Jyoti Joshi
Walter de Gruyter GmbH
Abstract A series of new 10H-phenothiazines, their sulfones, and ribofuranosides were synthesized and their in vitro antimicrobial assessment was carried out against a representative panel of Gram-positive and Gram-negative bacterial strains and selected fungi species. The 10H-phenothiazines were prepared using Smiles rearrangement. The sulfone derivatives were synthesized by oxidation of 10H-phenothiazines using 30% hydrogen peroxide in glacial acetic acid. The ribofuranosides were prepared by treatment of the phenothiazines with β-d-ribofuranosyl-1-acetate-2,3,5-tribenzoate.
JYOTI JOSHI and SUKHBIR KAUR
Cambridge University Press (CUP)
SUMMARYIt is well established that visceral leishmaniasis (VL; also known as Kala azar) causes immunosuppression, and a successful drug treatment is associated with the development of cell-mediated immunity. Therefore combining a drug with an immune enhancer can provide a better approach for the treatment of the disease. Keeping this in mind, the in vivo antileishmanial efficacy of immunochemotherapy was evaluated with the use of a 78 kDa antigen with or without monophosphoryl lipid A (MPL-A) along with a traditional drug sodium stibogluconate (SSG) in Leishmania donovani infected BALB/c mice. Mice were infected intracardially with promastigotes of L. donovani, and 30 days after infection, these animals were given specific immunotherapy (78 kDa/78 kDa+MPL-A) or chemotherapy (SSG) or immunochemotherapy (SSG+78 kDa/SSG+78 kDa+MPL-A). Animals were euthanased on 1, 15 and 30 post-treatment days. The antileishmanial potential of the immunochemotherapy was revealed by significant reduction in the parasite burden (P<0·001). These animals were also found to exhibit increased delayed type hypersensitivity (DTH) responses, higher IgG2a levels, lower IgG1 levels and greater cytokine (IFN-γ and IL-2) concentrations compared with chemotherapy or immunotherapy alone, pointing towards the generation of a strong protective (Th1) type of immune response. Immunochemotherapy with SSG+78 kDa+MPL-A was found to be most effective in protecting mice against VL and therefore can be an alternative option for treatment of VL.
Anshu Dandia, Ruby Singh, Jyoti Joshi, and Sukhbeer Kumari
Bentham Science Publishers Ltd.