Interleukin 17 expression in periapical lesions from elderly patients Rodolfo de Souza Rocha Júnior, Bruno de Oliveira Bergamin, Adriana Gomes Rocha Bastos, Alexandre Marques Paes da Silva, Fábio Ramôa Pires, et al. Special Care in Dentistry, 2024 ObjectiveTo compare the expression of IL‐17 in periapical lesions (cysts and granulomas) among elderly individuals and adults. We selected 20 periapical lesions of the elderly (12 granulomas and eight cysts) and 20 periapical lesions of adults (12 granulomas and eight cysts).Materials and MethodsImmunohistochemistry was performed using a specific antibody for IL‐17. The slides were subdivided into five high magnification fields and then the images were observed through an optical microscope. According to the number of positive markings for the antibody, grades were given, ranging from 0 to 2.ResultsThe results demonstrate that there was no statistical difference when comparing the expression of IL‐17 between cysts and granulomas of both groups (study group: cysts 0.7 ± 0.21 × granulomas 0.96 ± 0.58, p = .61; control group: cysts 0.37 ± 0.16 × granulomas 0.31 ± 0.23, p = .27). The comparison between adult (control group) and elderly patients (study group) showed a statistical difference both in cysts (study group: 0.7 ± 0.21 × control group: 0.37 ± 0.16, p = .007) and in granulomas (study group: 0.96 ± 0.58 × control group: 0.31 ± 0.23, p = .0009), in which elderly patients had a higher expression of interleukin 17, in relation to adult patients.ConclusionWe concluded that elderly patients have a higher expression of IL‐17 in both cysts and granulomas, when compared to adult patients.
Proinflammatory Cytokine Expression in Apical Periodontitis from Diabetic Patients Estéfano Borgo Sarmento, Rosana Helena Teixeira de Lima Ribeiro Andrade, Cinthya Cristina Gomes, Fábio Ramôa Pires, Maria Isabel Bastos Valente, et al. International Journal of Dentistry, 2023 Diabetes mellitus has been regarded as a condition capable of influencing the evolution of periapical lesions. Therefore, this study evaluated the immunoexpression of IL-1β, TNF-α, and IL-17 in apical periodontitis from type 2 diabetic patients through immunohistochemistry. Twenty-six periapical lesions were selected, and the images obtained through immunohistochemistry reactions were analyzed. The statistical significance was set at p < 0.05 . IL-1β expression was considered focal (54%), weak to moderate (38%), and strong (8%) in diabetics, and focal (46%), weak to moderate (31%), and strong (23%), in controls. TNF-α was focal (85%) and weak to moderate (15%) in diabetics, and focal (92%) and weak to moderate (8%), in controls. IL-17 was focal (8%), weak to moderate (46%), and strong (46%) in diabetics, and focal (62%), weak to moderate (8%), and strong (30%), in controls. The quantitative analysis revealed greater expression of IL-17, with a significant difference between IL-17 × IL-1β × TNF-α p = 0.0009 in the diabetic group. The cytokines IL-1β and TNF-α did not express statistical differences between the tested groups. The IL-17 showed higher immunoexpression in the diabetic group p = 0.047 , which may suggest higher bone resorption activity in chronic apical periodontitis in this group of patients.
Ameloblastomas mimicking apical periodontitis: a case series G. Soimu, Jd. Netto, ÁM. Miranda, DE. Perez, L. Armada, et al. Medicina Oral Patologia Oral Y Cirugia Bucal, 2022 Background Ameloblastomas are benign odontogenic tumors that can eventually mimic the clinical and radiological features of apical periodontitis. The aim of the present study was to evaluate the clinical, radiological and histological characteristics from a series of ameloblastomas mimicking apical periodontitis diagnosed in a 14-year period. Material and Methods all cases histologically diagnosed as ameloblastomas from 2005 to 2018 presenting a clinical diagnosis of periapical lesion of endodontic origin were selected for the study. Clinical, radiological and histological characteristics from all cases were tabulated and descriptively and comparatively analyzed. Results Twenty cases composed the final sample, including 18 solid and 2 unicystic ameloblastomas. Mean age of the affected patients was in the fifth decade with predilection for males (72%). The most common anatomical location was the posterior mandible (55%) and most cases presented a radiolucent unilocular (80%) well-defined (95%) image. Most cases were asymptomatic, but the presence of local swelling and bone cortical rupture were common. Conclusions Ameloblastomas mimicking periapical lesions of endodontic origin are mostly diagnosed in adult males as well-defined radiolucent unilocular lesions producing local swelling and bone cortical rupture. Key words:Ameloblastoma, apical periodontitis, cyst, differencial diagnosis, granuloma, periapical lesion.
Expression of epithelial growth factors and of apoptosis-regulating proteins, and presence of CD57+ cells in the development of inflammatory periapical lesions Walter Arthur Silva Valente, Déborah Barrocas, Luciana Armada, Fábio Ramôa Pires Journal of Applied Oral Science, 2022 The mechanisms that stimulate the proliferation of epithelial cells in inflammatory periapical lesions are not completely understood and the literature suggests that changes in the balance between apoptosis and immunity regulation appear to influence this process. Objective: To evaluate the expression of the epidermal growth factor (EGF), its receptor (EGFR) and of the keratinocyte growth factor (KGF), the presence of CD57+ cells, the epithelial cell proliferation index, and the expression of the Bcl-2 protein in inflammatory periapical lesions (IPL) at different stages of development. Methodology: Our sample was composed of 52 IPLs (22 periapical granulomas - PG - and 30 periapical cysts - PC), divided into three groups: PGs, small PCs, and large PCs. Specimens were processed for histopathologic and immunohistochemical analyses. Sections were evaluated according to the amount of positive staining for each antibody. Results: We found no significant differences among the groups regarding Bcl-2 (p=0.328) and Ki-67 (p>0.05) expression or the presence of CD57+ cells (p=0.748). EGF (p=0.0001) and KGF (p=0.0001) expression was more frequent in PCs than in PGs, and CD57+ cells were more frequent in IPLs with intense inflammatory infiltrates (p=0.0001). We found no significant differences in KGF (p=0.423), Bcl-2 (p=0.943), and EGF (p=0.53) expression in relation to inflammatory infiltrates or to the type of PC epithelial lining, but observed greater KGF expression (p=0.0001) in initial PCs. EGFR expression was similar among the groups (p>0.05). Conclusion: More frequent EGF and KGF expression in PCs and the greater presence of CD57+ cells in lesions with intense inflammatory infiltrates suggest that these factors influence IPL development. The greater KGF expression in initial PCs suggests its importance for the initial stages of PC formation.
Aging as a risk factor on the immunoexpression of pro-inflammatory il-1β, il-6 and tnf-α cytokines in chronic apical periodontitis lesions Quésia Euclides Teixeira, Dennis de Carvalho Ferreira, Alexandre Marques Paes da Silva, Lucio Souza Gonçalves, Fabio Ramoa Pires, et al. Biology, 2022 Persistent inflammatory responses in the elderly may act as modifiers on the progression and repair of chronic apical periodontitis lesions (CAPLs). While the involvement of IL-1β, IL-6 and TNF-α in inflammatory responses and, particularly, in CAPL has been documented, their expression in elderly patients needs to be further characterized. Therefore, the purpose of this study was to evaluate and compare the expressions of pro-inflammatory cytokines in CAPL from elderly individuals with young/middle-aged individuals. Thirty CAPL (15 cysts and 15 granulomas) from elderly patients (>60 years) and 30 CAPL (15 cysts and 15 granuloma) from young/middle-aged individuals (20–56 years) were selected. Immunohistochemical reactions were performed against IL-1β, IL-6 and TNF-α. The slides were subdivided into five high-magnification fields and analyzed. The number of positive stains was evaluated for each antibody. There was no significant difference between the cytokines when the cysts and granuloma were compared in the two groups. In the young/middle-aged, only IL-1β showed a difference and was significantly higher in granulomas (p = 0.019). CAPL pro-inflammatory cytokine levels in the elderly were significantly higher than in young/middle-aged individuals (p < 0.05). The pro-inflammatory cytokines IL-1β, IL-6 and TNF-α were significantly higher in CAPL in the elderly compared with the young/middle-aged group. Further elaborate research studies/analyses to elucidate the reasons for and consequences of inflammation in the elderly are recommended.
Tuberculose: o que existe de novo no tratamento e acompanhamento clínico DC de Vasconcelos Rios, VC Rios, CM Lins, FS Azevedo, IP da Silva, ... Enfermagem Brasil 24 (4), 2691-2701 , 2025 2025
Bidirectional association between dental caries and body mass index in socially vulnerable adolescents: a group-based trajectory analysis BML Gonçalves, L Armada, TH Stea, MAB Rebelo, JC Carvalho, ... Journal of Dentistry, 106087 , 2025 2025
EPIDEMIOLOGICAL SURVEY OF CASES OF AVULSION IN A BRAZILIAN POPULATION OVER 20 YEARS L de Oliveira Andrade, CGVF Pinto, C Gonçalves, RC de Souza, ... Revista DCS 22 (81), e3276-e3276 , 2025 2025
A primeira impressão humana é a que fica: ponto de vista sobre Inteligência Artificial na Medicina MA Orsini, MV Ghiggi, MRG de Freitas, CHM Reis, MA Araújo-Leite, ... Enfermagem Brasil 24 (3), 2541-2546 , 2025 2025
Veja as Flores! Onde estão os frutos? MA Orsini, MRG de Freitas, CHM Reis, AB Oliveira, L Armada Enfermagem Brasil 24 (3), 2373-2374 , 2025 2025
A dor que a rua cala: desigualdade, saúde e vulnerabilidade em um relato de caso MV Ghiggi, CHM Reis, MAA Leite, MRG de Freitas, V da Silveira Rocha, ... Enfermagem Brasil 24 (2), 2364-2372 , 2025 2025
Interleukins 6, 17 and 18 immunoexpression in oral lichen planus: association with clinical and histological parameters KH de Andrade Costa, JNS Netto, L Armada, RG Fischer, FR Pires Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology 139 (5), e133 , 2025 2025
Comparative histological and immunohistochemical study of Sjögren's syndrome and nonspecific chronic sialadenitis FR Pires, SM Amaral, BMM Caiafa, L Armada, JNS Netto, ... Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology 139 (5), e136 , 2025 2025
Osteopontin expression in oral potentially malignant disorders and oral squamous cell carcinoma MTL Ribeiro, RCP Baesso, L Armada, SN de Aquino, PA Vargas, ... Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology 139 (5), e136 , 2025 2025
O impacto das artes marciais como terapia suplementar para crianças com TDAH M Orsini, MV Ghiggi, R Gracie, R Gracie, ARC Nogueira, CAM Vieira, ... Educação e Inclusão 13 (1), 162-167 , 2025 2025
DOENÇAS PRIÔNICAS: FISIOPATOLOGIA E TRATAMENTO INTERDISCIPLINAR M Orsini, DMG Montano, SV Neves, FJ Silva, MP dos Santos, L Armada, ... ARACÊ 7 (3), 10982-10987 , 2025 2025
MANIFESTAÇÕES NEUROLÓGICAS DO HIV: UPDATE M Orsini, DMG Montano, SV Neves, FJ Silva, MP dos Santos, L Armada, ... ARACÊ 7 (2), 8604-8610 , 2025 2025
Autolesões e suicidabilidade em transtorno de personalidade borderline associado a transtorno de parafilia: um relato de caso T de Mello Tavares, M Orsini, B McBenedict, MH Magnani, ... Revista Caribeña-QUALIS B1 14 (1), e4422-e4422 , 2025 2025
TRATAMENTO DO TDAH EM ADOLESCENTES: RELATO DE CASO SOBRE A INTEGRAÇÃO DE TERAPIA FARMACOLÓGICA E ESPORTE DE PRECISÃO T de Mello Tavares, M Orsini, L Armada, MH Magnani Editora Impacto Científico, 670-678 , 2025 2025
GLIOBLASTOMAS AND THE DILEMMA OF ITS TREATMENT: A PHILOSOPHICAL PERSPECTIVE M Orsini, BL Pessôa, L Adolphsson, B McBenedict, JGM Netto, ... Anais New Science Publishers| Editora Impacto , 2024 2024
Radiographic evaluation of the effects of a high-fat-diet on mandibular bone in animals KMM da Costa Pinto, VM da Encarnação, DGP Dantas, MJ de Oliveira, ... Nutrição Brasil 23 (4), 1097-1107 , 2024 2024
HEMANGIOBLASTOMA CEREBELAR E LEUCEMIA LINFÓIDE AGUDA: UMA ASSOCIAÇÃO CASUAL OU GENETICAMENTE DETERMINADA? M Orsini, BL Pessôa, L Adolphsson, B McBenedict, JGM Netto, ... ARACÊ 6 (3), 6196-6201 , 2024 2024
Alterações do Paladar em Crianças: Análise das Informações Disponíveis em uma Plataforma Virtual A Pinho, K Ferreira, N Mecler, L Armada, D Ferreira Revista de Saúde 15 (2), 40-47 , 2024 2024
Efeitos gastrointestinais da exposição ao hexaclorociclohexano no organismo humano CAM Vieira, AM Moreno, MS Tavares, BR Benevenuto, T dos Santos Reis, ... Cuadernos de Educación y Desarrollo-QUALIS A4 16 (8), e5356-e5356 , 2024 2024
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MOST CITED SCHOLAR PUBLICATIONS
Type 2 diabetes mellitus and the prevalence of apical periodontitis and endodontic treatment in an adult Brazilian population PS Marotta, TV Fontes, L Armada, KC Lima, IN Rôças, JF Siqueira Jr Journal of endodontics 38 (3), 297-300 , 2012 2012 Citations: 235
Glide path preparation in S-shaped canals with rotary pathfinding nickel-titanium instruments NCC Ajuz, L Armada, LS Gonçalves, G Debelian, JF Siqueira Jr Journal of endodontics 39 (4), 534-537 , 2013 2013 Citations: 126
Distribution of mast cells and macrophages and expression of interleukin-6 in periapical cysts IV Bracks, L Armada, LS Gonçalves, FR Pires Journal of endodontics 40 (1), 63-68 , 2014 2014 Citations: 70
Pro-inflammatory and anti-inflammatory cytokine expression in post-treatment apical periodontitis N Dessaune Neto, MTM Porpino, HS Antunes, RCV Rodrigues, AR Perez, ... Journal of Applied Oral Science 26, e20170455 , 2018 2018 Citations: 69
Princípios biológicos do tratamento endodôntico de dentes com polpa necrosada e lesão perirradicular JF Siqueira Jr, IN Rôças, HP Lopes, FRF Alves, JCM Oliveira, L Armada, ... Revista Brasileira de Odontologia 69 (1), 08 , 2012 2012 Citations: 67
Immunoexpression of interleukin 17 in apical periodontitis lesions NC Ajuz, H Antunes, TA Mendonça, FR Pires, JF Siqueira Jr, L Armada Journal of endodontics 40 (9), 1400-1403 , 2014 2014 Citations: 65
Incorporation and remodeling of bone block allografts in the maxillary reconstruction: A randomized clinical trial D Deluiz, L Santos Oliveira, F Ramôa Pires, T Reiner, L Armada, ... Clinical implant dentistry and related research 19 (1), 180-194 , 2017 2017 Citations: 45
Expression and distribution of receptor activator of nuclear factor kappa B, receptor activator of nuclear factor kappa B ligand, and osteoprotegerin in periradicular cysts L Armada, P dos Santos Marotta, FR Pires, JF Siqueira Jr Journal of endodontics 41 (8), 1281-1287 , 2015 2015 Citations: 42
Transportation assessment in artificial curved canals after instrumentation with Reciproc, Reciproc Blue, and XP‐endo Shaper Systems J Pacheco‐Yanes, I Gazzaneo, AR Pérez, L Armada, MAS Neves Journal of investigative and clinical dentistry 10 (3), e12417 , 2019 2019 Citations: 37
Influence of oestrogen deficiency on the development of apical periodontitis SC Brasil, RMM Santos, A Fernandes, FRF Alves, FR Pires, JF Siqueira Jr, ... International endodontic journal 50 (2), 161-166 , 2017 2017 Citations: 34
Aging as a Risk Factor on the Immunoexpression of Pro-Inflammatory IL-1β, IL-6 and TNF-α Cytokines in Chronic Apical Periodontitis Lesions QE Teixeira, DC Ferreira, AMP da Silva, LS Gonçalves, FR Pires, ... Biology 11 (1), 14 , 2021 2021 Citations: 32
Influence of a High-fat Diet in the Progression of Apical Periodontitis SC Brasil, RMM Santos, A Fernandes, RS Lima, CAS Costa, ... Journal of Endodontics 47 (4), 600-605 , 2021 2021 Citations: 31
Clinical and radiological analysis of a series of periapical cysts and periapical granulomas diagnosed in a Brazilian population DP Tavares, JT Rodrigues, L Armada, FR Pires Journal of clinical and experimental dentistry 9 (1), e129 , 2017 2017 Citations: 28
Mandible analysis in sex steroid‐deficient rats L Armada, CRR Nogueira, UL Neves, P dos Santos Souza, JP Detogne, ... Oral diseases 12 (2), 181-186 , 2006 2006 Citations: 28
Immunoexpression of Wnt/β-catenin signaling pathway proteins in ameloblastoma and calcifying cystic odontogenic tumor SN Dutra, FR Pires, L Armada, RS Azevedo Journal of clinical and experimental dentistry 9 (1), e136 , 2017 2017 Citations: 26
Immunoexpression of bone resorption biomarkers in apical periodontitis in diabetics and normoglycaemics EB Sarmento, CC Gomes, FR Pires, LC Pinto, LAA Antunes, L Armada International endodontic journal 53 (8), 1025-1032 , 2020 2020 Citations: 24
Efficacy of three methods for inserting calcium hydroxide-based paste in root canals T Galvão, B Camargo, L Armada, F Alves Journal of clinical and experimental dentistry 9 (6), e762 , 2017 2017 Citations: 24
Influence of surgical decompression on the expression of inflammatory and tissue repair biomarkers in periapical cysts JT Rodrigues, H dos Santos Antunes, L Armada, FR Pires Oral surgery, oral medicine, oral pathology and oral radiology 124 (6), 561-567 , 2017 2017 Citations: 21
Distribution of macrophages and plasma cells in apical periodontitis and their relationship with clinical and image data SV Azeredo, SC Brasil, H Antunes, FV Marques, FR Pires, L Armada Journal of clinical and experimental dentistry 9 (9), e1060 , 2017 2017 Citations: 20
Princípios biológicos do tratamento endodôntico de dentes com polpa viva JF Siqueira Jr, IN Rôças, HP Lopes, FRF Alves, JCM Oliveira, L Armada, ... Revista Brasileira de Odontologia 68 (2), 161 , 2012 2012 Citations: 16