Max Roberto Batista de Araujo
@hermespardini.com.br
Microbiology
Hermes Pardini Institute
He holds a degree in Pharmacy-Biochemistry from the Federal University of Ouro Preto (2008). From 2009 to the present, she has worked as a Full Laboratory Specialist at the Hermes Pardini S / A Institute. Has relevant experience in microbiological diagnoses by manual and automated methodologies (VITEK II and Vitek MS). Responsible for automated outpatient and inpatient examinations performed by Mass Spectrometry.
EDUCATION
Universidade Federal de Ouro Preto
Universidade Federal de Minas Gerais
RESEARCH INTERESTS
Global Health Infectious Disease
Epidemiology
Epidemiological Analysis
Tuberculosis Infectious
Disease Control and Prevention Clinical Infectious Diseases
Emerging Infectious Diseases
Infectious Disease Transmission
Tropical Diseases
Drug Resistance
Infectious Disease Diagnostics
Scopus Publications
Scholar Citations
Scholar h-index
Scopus Publications
Juliana Nunes Ramos, Max Roberto Batista Araújo, Paulo Victor Pereira Baio, Lincoln Oliveira Sant’Anna, João Flávio Carneiro Veras, Érica Miranda Damásio Vieira, Mireille Ângela Bernardes Sousa, Carlos Henrique Camargo, Cláudio Tavares Sacchi, Karoline Rodrigues Campos,et al.
Springer Science and Business Media LLC
Abstract Background Corynebacterium diphtheriae complex was formed by the species C. diphtheriae, Corynebacterium ulcerans and Corynebacterium pseudotuberculosis in the recent past. In addition to C. diphtheriae, C. ulcerans and C. pseudotuberculosis species can carry the tox gene, which encodes diphtheria toxin. Currently, three new species have been included in the complex: Corynebacterium rouxii, Corynebacterium silvaticum, and Corynebacterium belfantii. C. rouxii is derived from the ancient Belfanti biovar of C. diptheriae. We provide the complete genome sequences of two non-toxigenic strains C. rouxii isolated from a cat with a purulent infection in Brazil. The taxonomic status and sequence type, as well as the presence of resistance and virulence genes, and CRISPR-Cas system were additionally defined. Results The genomes showed an average size of 2.4 Mb and 53.2% GC content, similar to the type strain of the species deposited in Genbank/NCBI. Strains were identified as C. rouxii by the rMLST database, with 95% identity. ANI and DDH in silico were consistent with values above the proposed cut-off points for species limit, corroborating the identification of the strains as C. rouxii. MLST analyses revealed a new ST, which differs from ST-537 only by the fusA allele. No horizontal transfer resistance gene was predicted in both genomes and no mutation was detected in the constitutive genes gyrA and rpoB. Some mutations were found in the seven penicillin-binding proteins (PBPs) detected. The tox gene was not found, but its regulatory gene dtxR was present. Among the predicted virulence genes are those involved in iron uptake and adherence, in addition to the DIP0733 protein involved in epithelial cell adhesion and invasion. The CRISPR-Cas type I-E system was detected in both genomes, with 16 spacer sequences each. Of them, half are unknown according to the databases used, indicating that there is an unexplored reservoir of corynebacteriophages and plasmids. Conclusions This is the first genomic study of C. rouxii reported in Brazil. Here we performed taxonomic analysis and the prediction of virulence factors. The genomic analyses performed in this study may help to understand the potential pathogenesis of non-toxigenic C. rouxii strains.
Max Roberto Batista Araújo, Juliana Nunes Ramos, Lincoln de Oliveira Sant’Anna, Sérgio Bokermann, Marlon Benedito Nascimento Santos, Ana Luiza Mattos-Guaraldi, Vasco Azevedo, Fernanda Diniz Prates, Diego Lucas Neres Rodrigues, Flávia Figueira Aburjaile,et al.
Springer Science and Business Media LLC
Louisy Sanches dos Santos, Lincoln de Oliveira Sant’Anna, Rafael Theodoro, Nadir Nayara Carvalho dos Santos, Bruna Karoline Lopes Armond, Luisa Ferreira Seabra, Luige Biciati Alvim, and Max Roberto Batista Araújo
Springer Science and Business Media LLC
Lincoln de Oliveira Sant’Anna, Louisy Sanches dos Santos, Max Roberto Batista Araújo, Danilo Jobim Passos Gil da Rocha, Juliana Nunes Ramos, Paulo Victor Pereira Baio, Pedro Fernandez Del Peloso, Cassiana da Costa Ferreira Leite, Renata Stavrakakis Peixoto, Marisa Almuzara,et al.
Springer Science and Business Media LLC
Juliana Nunes Ramos, Max Roberto Batista Araújo, Lincoln Oliveira Sant’Anna, Sérgio Bokermann, Carlos Henrique Camargo, Fernanda Diniz Prates, Cláudio Tavares Sacchi, Verônica Viana Vieira, Karoline Rodrigues Campos, Marlon Benedito Nascimento Santos,et al.
Springer Science and Business Media LLC
Max Roberto Batista Araújo, Lincoln de Oliveira Sant’Anna, Nadir Nayara Carvalho dos Santos, Luisa Ferreira Seabra, and Louisy Sanches dos Santos
FapUNIFESP (SciELO)
ABSTRACT Background: Bacterial resistance to extended-spectrum beta-lactamases (ESBL) is present worldwide. Empirical antibiotic therapy is often needed, and the use of fluoroquinolones, such as ciprofloxacin and norfloxacin, is common. This study aimed to analyze the urine cultures from 2,680 outpatients in January 2019, 2020, 2021, and 2022, with bacterial counts above 100,000 CFU/mL in which Escherichia coli was the etiological agent. Methods: We monitored the resistance of ESBL-positive and ESBL-negative strains to ciprofloxacin and norfloxacin and evaluated resistance rates. Results: Significantly higher fluoroquinolone resistance rates were observed among ESBL-positive strains in all years studied. Furthermore, a significant increase in the rate of fluoroquinolone resistance was observed between 2021 and 2022 in ESBL-positive and -negative strains, as well as from 2020 to 2021 among the ESBL-positive strains. Conclusions: The data obtained in the present study showed a tendency towards an increase in fluoroquinolone resistance among ESBL-positive and -negative E. coli strains isolated from urine cultures in Brazil. Since empirical antibiotic therapy with fluoroquinolones is commonly used to treat diverse types of infections, such as community-acquired urinary tract infections, this work highlights the need for continuous monitoring of fluoroquinolone resistance among E. coli strains circulating in the community, which can mitigate the frequency of therapeutic failures and development of widespread multidrug-resistant strains.
Lincoln de Oliveira Sant’Anna, Elisabete Alves Cappelli, Max Roberto Batista Araújo, Juliana Nunes Ramos, Liliane Simpson-Lourêdo, Andrezza do Espirito Santo Cucinelli, Paulo Victor Pereira Baio, Verônica Viana Vieira, Louisy Sanches dos Santos, and Ana Luíza Mattos-Guaraldi
Elsevier BV
Lincoln de Oliveira Sant’Anna, Louisy Sanches dos Santos, and Max Roberto Batista Araújo
FapUNIFESP (SciELO)
A 22-year-old Brazilian man with untreated ulcerative colitis and human immunodeficiency virus (HIV) infection was admitted to the intensive care unit (ICU) for holocranial headache, fever, nausea, malaise, and diarrhea. Antiretroviral therapy (ART) was discontinued eight months earlier. Medical history included prior hospitalization due to pneumomediastinum secondary to perforated esophageal moniliasis. Laboratory tests showed leukocytosis, elevated C-reactive protein levels, high HIV viral load (133.627 copies/mL), low CD4+T-cell count (14 cells/mm3), and abnormal levels of cerebrospinal fluid (CSF) proteins and glucose. Treatment with ceftriaxone, metronidazole, and mebendazole was initiated. Further CSF analysis was negative for Cryptococcus spp., but fungal culture showed the growth of black, rough colonies (Figure 1a) with conidia as observed by optical microscopy (Figure 1b). The fungus was identified as Aureobasidium melanogenum using gene sequencing. Treatment with amphotericin B lipid complex was initiated for 14 days, and he was discharged from the ICU after re-initiating ART.
Louisy Sanches dos Santos, Lincoln de Oliveira Sant’Anna, and Max Roberto Batista Araújo
FapUNIFESP (SciELO)
FIGURE 1: Giemsa staining (original magnification, ×1000) of bronchoalveolar fluid showing cyst forms of Pneumocystis jirovecii (black arrow). A 54-year-old Brazilian man presented to the emergency department with cough, chest pain, high fever, and dyspnea. He had no history of sexually transmitted infections. A thoracic computed tomography scan showed ground-glass opacification areas and mediastinal lymphadenopathy. Laboratory tests revealed the following abnormalities: absolute monocyte count, 80 cells/mm3; partial pressure of oxygen, 55.3 mmHg; and C-reactive protein,304.3 mg/L. Blood tests for cytomegalovirus, Chlamydia pneumoniae , Legionella pneumophila , and Mycoplasma pneumoniae and a sputum analysis for Mycobacterium tuberculosis were negative. Microscopic examination of Giemsastained bronchoalveolar lavage fluid (BALF) showed cysts of the atypical fungus Pneumocystis jirovecii (Figure 1), the etiological agent of pneumocystis pneumonia (PCP). Additional investigations revealed human immunodeficiency virus (HIV) infection, low CD4+T-cell count (128 cells/mm3), and increased lactate dehydrogenase levels. Antiretroviral therapy (ART) and trimethoprim/sulfamethoxazole (14 days) treatment were established. The patient was discharged 30 days post-admission.
Max Roberto Batista Araújo, Lincoln Oliveira Sant’Anna, and Louisy Sanches dos Santos
FapUNIFESP (SciELO)
FIGURE 1: Giemsa staining (original magnification, ×1000) showing Donovan bodies (black arrow). A 33-year-old man presenting with ulcerated and painless anal lesions was seen by his general practitioner in Belo Horizonte City, Minas Gerais State, Brazil. Screening tests for sexually transmitted infections (STIs) and microscopic examinations of swabs of ulcer material were conducted. Serological examinations gave positive results for human immunodeficiency virus (HIV-1), herpes simplex virus (HSV-1/2), Treponema pallidum, and Chlamydia trachomatis infections. A microscopic analysis by Giemsa staining showed negative results for Tzank or Haemophilus ducreyi; however, it showed Donovan bodies that are characteristic of donovanosis (Figure 1).