Food Science, General Agricultural and Biological Sciences
7
Scopus Publications
Scopus Publications
Antioxidant activity and bioactive compounds in ora-pro-nóbis flour (Pereskia aculeata Miller) Ariane Cristina de Almeida Ciríaco, Ricardo de Morais Mendes, Vania Silva Carvalho Brazilian Journal of Food Technology, 2023 The ora-pro-nóbis (Pereskia aculeata Miller) is a cactus popularly known as “poor man's meat” due to its popular use related to the protein content found in its leaves and its low cost, thus being classified as an Unconventional Food Plant (UFP). The plant is recognized for the high nutritional value of their leaves, and it can be used in cooking and folk medicine. However, studies on the chemical characteristics and antioxidant capacity of ora-pro-nóbis fruits are scarce. This study aimed to evaluate the antioxidant potential and phenolic compounds of the stem and leaf flours and fruit pulp of P. aculeata Miller. The stem and leaves were washed and dried in a circulation oven at 60° ± 2 °C, and crushed in an industrial blender. The fruit was washed and macerated in natura, then hydroethanolic extracts were prepared. The content of phenolic compounds was evaluated using the Folin-Ciocalteau reagent and the results showed a total phenol content in the stem, leaves and fruits of 0.25; 1.01 and 118.2 mg EAG g-1 of extract, respectively. Antioxidants were evaluated using the DPPH method, obtaining values for stem, leaf and fruit of 1.20; 1.40 and 1.50 mg of fruit g-1 of DPPH respectively, and also by the ABTS method of stem, leaf and fruit of:13.82; 6.30; 3.20 µmol of Trolox g-1, respectively. Thus, P. aculeata Miller presented in its stem, leaves and fruits an expressive amount of phenolic and antioxidant compounds, being a potential resource to contribute to the prevention of several disorders associated with the production of free radicals as well as an alternative in the enrichment of foods and being a useful source for a food additive.
Potential of thyme essential oil on arugula sanitization Roseli Jacobi Veloso, Nei Fronza, Alvaro Vargas Júnior, Vânia Silva Carvalho, Miriam Fumiko Fujinawa, et al. Ciencia E Agrotecnologia, 2019 Essential oils from plants are an important alternative source of antimicrobial compounds against important pathogens for food safety. This study aimed to evaluate the in vitro antimicrobial activity of thyme essential oil (Thymus vulgaris) and to apply it in the sanitization of minimally processed arugula (Eruca sativa), aiming to increase the safety and shelf life of arugula. The in vitro antibacterial activity of the essential oil was evaluated by disc diffusion assays and determining the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against different foodborne pathogens. Thereafter, a “challenge test” was carried out to evaluate the antibacterial activity of the thyme essential oil (0.2%) on the sanitization of arugula inoculated with Escherichia coli (3 log CFU mL-1) compared to sodium hypochlorite (120 ppm of active chlorine). Afterwards, an experiment of applying the thyme essential oil (0.2%) as a sanitizing agent in minimally processed arugula was carried out to evaluate the vegetable shelf life compared to sodium hypochlorite (120 ppm of active chlorine). The arugula was stored at 8 °C for 7 days, and periodically, the number of aerobic mesophilic microorganisms, pH and titratable acidity were determined, and the color of the samples was evaluated. The thyme essential oil had strong antimicrobial activity in vitro against all species of bacteria tested. In the experiments in situ, the thyme essential oil (0.2%) and sodium hypochlorite (120 ppm of active chlorine) were not efficient in eliminating the inoculated E. coli from minimally processed arugula, and they did not result in an extension of the shelf life of the arugula in comparison to the control (p>0.05). However, the growth rate of the E. coli population over time on the arugula samples sanitized with the thyme essential oil (0.2%) was low, which indicates a positive effect of the essential oil, which may be optimized with future adjustments in the concentration and immersion time for the arugula sanitization.
Storage study of cereal bars formulated with banana peel flour: Bioactive compounds and texture properties Vania Silva Carvalho, Ana Carolina Conti-Silva Nutrition and Food Science, 2018 Purpose This paper aims to examine the bioactive compounds and texture properties of cereal bars formulated with banana peel flour during storage. Design/methodology/approach Seven cereal bars were produced and stored during 11 months, under vacuum and protected from the light. The total phenolic compounds, the activity antioxidant by ABTS [2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)] method, the DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) method expressed as EC50 (concentration required to reduce the original amount of free radicals by 50 per cent) and texture properties were evaluated over the storage period. Findings In general, total phenolic compounds decreased during storage (from 4.19 to 1.11 mg GAE. g-1f.w.). Although the total antioxidant activity (ABTS method) increased during the fifth month, it reduced during storage (from 3.41 to 0.30 µmol TE.g-1f.w.); and the EC50 was not modified in many formulations, though it decreased in other formulations during storage period (from 3913 to 19221 g fruit.g-1 DPPH). The force of rupture began to increase in the fourth month (reaching 62.4 N), and hardness began to increase in the ninth month (reaching 444 N). The formulation and time factors influenced the total phenolic compounds, total antioxidant activity (ABTS method), force of rupture and hardness, while EC50 was only influenced by the formulation (p-value = 0.001). A principal component analysis showed that time had little effect on the most important characteristics considered in description of the cereal bars. Originality/value Cereal bars can be consumed up to the third month of storage, considering the texture of the products. Moreover, the presence of bioactive compounds in cereal bars depends on the addition of banana peel flour, which it contributes to the insertion of total phenolic compounds and total antioxidant activity in cereal bars, aggregating functional properties in these products.
Measuring dry extract in dairy products: Optimizing the methodology Vanessa Cristina Martins, Suzane Martins Ferreira, Vania Silva Carvalho Ciencia Rural, 2018 Dairy products are a common staple in the human diet. Moisture content is an important factor in the composition of foods, and it is a parameter used to measure product quality due to its direct influence on food storage. In this study, the moisture contents of three different products (mozzarella cheese, a creamy cheese spread, and yogurt drinks) were measured using oven drying, infrared drying, and microwave drying in order to compare the results of these three methods. Three brands of each product were used, all of which were purchased at the local supermarket in the city of Goiatuba, Goiás, Brazil. The different methods used to measure the dry extract of most of the brands analyzed did not differ significantly between each other based on a significance level of 5% on Tukey’s test. There was a significant difference between the methodologies used in the case of only one brand of each analyzed product. Microwave drying method wasreported to be a safe and reliable technique for analyzing dry extract from dairy products; it produces precise results, thus guaranteeing the quality of the analysis, and it reduces procedure time, thus optimizing the routine analytical processes performed in quality control laboratories within food processing plants.
Use of sugar apple, atemoya and soursop for Technological Development of jams - Chemical and sensorial composition Daniela Castilho Orsi, Vania Silva Carvalho, Adriana Cândida Faustino Nishi, Clarissa Damiani, Eduardo Ramirez Asquieri Ciencia E Agrotecnologia, 2012 Sugar apple, atemoya and soursop are tropical fruits very prized for their pleasant, aromatic and distinctive flavor. In this work, the fresh fruits pulps had high nutritional values, since they contain significant levels of carbohydrates. The results for the analysis of chemical composition of the fresh fruits pulps obtained for sugar apple, atemoya and soursop were respectively: moisture (79.65, 77.06 and 85.30 g.100 g-1), carbohydrates (20.52, 22.01 and 13.83 g.100 g-1), total sugars (19.57, 21.93 and 10.12 g.100 g-1) and soluble solids (22.00, 25.10 and 12.83 ºBrix). With the aim of processing the pulps of ripe fruits to add value to these fruits, jams have been developed. The results for the chemical composition analysis of sugar apple, atemoya and soursop jams were respectively: moisture (26.23, 27.94 and 21.52 g.100 g-1), carbohydrates (73.05, 71.44 and 77.83 g.100 g-1), pH (3.41, 3.34 and 3.41) and soluble solids (63.00, 65.00 and 68.60 ºBrix). The jams were subjected to Quantitative Descriptive Analysis. A total of 12 trained panelists evaluated the attributes flavor, consistency, appearance and overall acceptability of the jams on a 9-point hedonic scale. Based on sensory evaluation, jams presented a good overall acceptability and atemoya jam was the most preferred by the panelists.
Development and antioxidant capacity of sapota pulp jelly (Quararibea cordata Vischer) Vania Silva Carvalho, Clarissa Damiani, Eduardo Ramirez Asquieri, Daniela Castilho Orsi, Adriana Cândida Faustino Nishi Ciencia E Agrotecnologia, 2012 Sapote (Quararibea cordata Vischer), also known as a chupa-chupa, is originated from the Brazilian, Peruvian and Colombian Amazon. The pulp of the ripe fruit is edible, fibrous, of intense orange color, sweet flavor and aromatic. Since the fruit is known in the Amazon only in its domesticated state, this work becomes necessary. With the aim of meeting the demand for new products in domestic and international markets, sapota pulp jelly has been developed. The jelly was assessed for moisture, ash, lipids, proteins, carbohydrates, dietary fiber, pectin, pH, total acidity, solids soluble, sugars, organic acids, carotenoids, phenolic compounds and antioxidant capacity. The final product met the standards required by Brazilian law, and 32.68% moisture and 61.06% °Brix. Also, in accordance with the laws attributed to this product, jelly sapota pulp can be considered a food rich in fiber, as presented content above 5%. The jelly, showed content of total phenolics compounds (102 mg GAE. 100 -1) in relation to fresh fruit (21 mg GAE.100 g-1). There was the identification in the antioxidant capacity (9.05% scavering of DPPH radical), giving the final product antioxidant properties. Also were analyzed the microbiological characteristics of the product which was not observed the presence of thermotolerants coliforms, yeast and molds. The sapota can be considered effective raw materials in the preparation of jam and this had an antioxidant activity and source of fiber.
