Knuckle Crease Patterns in Forensic Biometrics: A Novel Trait for Identity Verification and Investigative Applications Prachi Sharma Biswas, Swati Dubey Mishra Exploring the Intersection of Forensics and Biometrics, 2026 Knuckle crease patterns, formed by natural skin folds over finger joints, are emerging as a valuable biometric trait in forensic science and digital identity. Often visible in photographs and surveillance footage, they aid identification when fingerprints or DNA are unavailable. This chapter reviews their anatomical foundations, persistence, and distinctiveness, and examines automated recognition methods from handcrafted descriptors to deep learning, alongside manual classification approaches emphasizing reproducibility. Forensic applications include criminal investigations, child sexual abuse material analysis, disaster victim identification, and multimodal biometrics. Ethical, legal, and evidentiary issues are discussed, and future challenges for validation and responsible implementation are highlighted.
Fingerprint analysis for the determination of hand origin (right/left) using the axis slant in whorl patterns Neeti Kapoor, Ashish Badiye, Swati Dubey Mishra Forensic Sciences Research, 2022 Fingerprints are frequently encountered during both civil and criminal investigations. Fingerprints possess numerous characteristics that assist with personal identification. Determining the hand of origin (right or left) for an individual fingerprint would help reduce investigation time and potentially eliminate certain suspects. In this study, we collected a total of 2 900 single digit fingerprints from 290 individuals, and the whorl axis slant was examined in the 743 whorl pattern fingerprints (385 from the right hand and 358 from the left hand). A slant towards the right side was present in 81.82% of samples from the right hand, while a slant towards the left side was observed in 80.73% of samples from the left hand. After applying a chi-square test to the dataset, the results were found to be statistically significant for the determination of hand origin. Our results suggest that the whorl axis slant in a fingerprint is indicative of hand origin (right or left). Key points Single digit fingerprints with whorl pattern were analyzed. Whorl “axis slant” was used to determine the hand origin. Right axis slant would indicate the right hand of the print. Left axis slant would indicate the left hand of the print.
Single-digit fingerprint analysis for hand determination: A study of twinned loops Neeti Kapoor, Swati Dubey Mishra, Ashish Badiye Medicine Science and the Law, 2020 In fingerprint analysis, the determination of hand (right or left) is one of the keys to resolving questions regarding the identity of the donor of the print. It is rare to encounter a complete set of 10-digit fingerprints from the scene of a crime. Although vital and imperative, except for a few studies on whorl patterns, the use of single-digit prints and patterns to ascertain the hand of the donor is scarcely reported in the literature. This research describes a novel approach whereby an analysis of twinned loops was carried out to identify the various parameters that may be helpful in ascertaining the hand of the donor. Bilateral rolled fingerprints from 180 healthy consenting adults belonging to a heterogeneous population of central India were collected. Out of the total of 1800 prints taken, 100 twinned loops (50 on the right hand and 50 on the left hand) were analysed. The following six parameters were considered: the slope of the flow of the apex ridges, the distance between the left delta and the ascending core, the distance between the right delta and the ascending core, mid-core position, the position of the loop and ridge tracing. On applying the chi-square test, the results were found to be statistically significant. Therefore, these parameters were found suitable for the determination of the hand from a single twinned-loop print.
Determination of alpha-beta arteether in pharmaceutical products using direct injection micellar liquid chromatography S. Dubey, D. Bose, S. K. Shukla, A. Durgbanshi, J. Esteve-Romero, S. Carda-Broch Journal of Liquid Chromatography and Related Technologies, 2014 A simple and easy liquid chromatographic procedure was developed for the separation and analysis of α,β-arteether. The developed method uses a reversed-phase Luna C18 column (250 mm × 4.6 mm), a hybrid micellar mobile phase containing 0.15 M SDS–6% (v/v) butanol–0.01 M phosphate buffered at pH 7, and detection at 218 nm. Under these conditions, the analysis time was less than 6 min. Validation studies were performed according to the International Conference on Harmonization guideline, including linearity (R2 > 0.9998) in the 0.1–50 µg/mL range, accuracy (99.8–100.8%), repeatability [RSD (%) 1.8–2.8], intermediate precision [RSD (%) 1.8–2.9], and robustness, which were found satisfactory. Method was applied to the determination of α,β-arteether in different pharmaceutical preparations and wrapper/label free ampoules obtaining recoveries in the 97.7–102.2% range. Compared to other chromatographic techniques, micellar liquid chromatography has the advantages of avoiding sample extraction from matrices, thus reducing the global analysis time and environmental toxicity.
Monitoring strychnine and brucine in biochemical samples using direct injection micellar liquid chromatography Swati Dubey Mishra, Devasish Bose, S. K. Shukla, Abhilasha Durgabanshi, J. Esteve-Romero Analytical Methods, 2013 Nux-vomica is a tropical plant that contains two ergot alkaloids i.e. strychnine and brucine which have pharmaceutical and toxic properties. In some places, for example in India, Nux-vomica is used in ayurvedic preparations in homeopathic medicine. As both compounds are toxic monitoring is required. In this work a method for the chromatographic determination of strychnine and brucine in various herbal/ayurvedic sample preparations, homeopathic medicines, containing Nux-vomica, seeds of Nux-vomica as well as in spiked serum and real urine samples are described. The method involves a direct injection micellar liquid chromatography technique that was optimised under ICH guidelines. Optimum conditions are a mobile phase composed by 0.10 M SDS-pentanol 4% (v/v)-NaH2PO4 buffered at pH 3 with UV detection at 258 nm, flow rate of 1 mL min−1, and temperature 25 °C. Under these conditions strychnine and brucine were analyzed in 15 and 11 min, respectively. Validation studies using ICH guidelines were performed to show that the proposed method was linear (r2 > 0.9997), with LODs (0.18 and 0.09) and LOQs (0.69 and 0.35), for strycnine and brucine, respectively. Repeatability intra- and inter-day was below of 2.50. The developed method can be useful for analyzing strychnine and brucine for quality control and other related forensic and clinical cases.
Determination of disulfiram by micellar liquid chromatography in illicit preparations Sandeep-Kumar mourya, Swati Dubey, Abhilasha Durgabanshi, Sudheer Kumar Shukla, Josep Esteve-Romero, Devasish Bose Journal of AOAC International, 2011 Presently, disulfram is used in aversion therapy for recovering alcoholics. It acts by inhibiting aldehyde dehydrogenase, leading to high blood levels of acetaldehyde. A simple direct injection micellar liquid chromatographic procedure was developed to determine disulfram in illicit preparations (ayurvedic, herbal, divine ash, and traditional medicine), as well as inpharmaceuticals and biological samples (urine). After application of a predictive optimization strategy, the proposed method was developed using a 0.1 M sodium dodecyl sulfate-butanol 4% (v/v) buffered to pH 7 as the mobile phase at a flow rate of 1 mL/min, an octyl silyl (C8) 150 mm column, and diode array detection at 248 nm. Under the above conditions, the analysis time was below 8 min. Validation studies were based on U.S. Food and Drug Administration guidelines. The LOD (3 × SD criterion) was 15 ng/mL and LOQ (10 × SD criterion) was 70 ng/mL for disulfram. The intraday and interday precisions were below 3.5%, recoveries were in the range of 97–102%, and robustness was below 3%. The optimized and validated micellar liquid chromatographic method was successfully applied to the determination of disulfram in ayurvedic, herbal, divine ash, and other samples. The procedure developed could also be used in the felds of QC, routine analysis, and pharmacokinetic studies.
RECENT SCHOLAR PUBLICATIONS
Knuckle Crease Patterns in Forensic Biometrics: A Novel Trait for Identity SD Mishra Exploring the Intersection of Forensics and Biometrics, 133 , 2026 2026
Development of a Green and Robust Micellar Liquid Chromatography Method for the Simultaneous Determination of Malathion and Carbofuran. N Ingole, SD Mishra, D Bose Journal of Chemical Health Risks 16 (1), 2495 , 2026 2026
Knuckle Crease Patterns in Forensic Biometrics: A Novel Trait for Identity Verification and Investigative Applications PS Biswas, SD Mishra Exploring the Intersection of Forensics and Biometrics, 133-166 , 2026 2026
Analysis of Palmar Creases for Personal Identification A Beragi, SD Mishra Integrating Interdisciplinary Research for Societal Well-Being, 74 , 2025 2025
The impact of neurological disorders on handwriting: Implications for forensic document examination H Bansal, SD Mishra, M Singhal Wiley Interdisciplinary Reviews: Forensic Science 6 (6), e1536 , 2024 2024 Citations: 3
An assessment of biometrics tools: Fingerprint and knuckle crease patterns PS Biswas, SD Mishra Fostering Multidisciplinary Research for Sustainability, 145 , 2024 2024 Citations: 1
Novel Approach For Personal Identification Using Dorsal Knuckle Crease Patterns: A Pilot Study PS Biswas, SD Mishra Mapana Journal of Sciences 23 (4), 83 , 2024 2024 Citations: 2
Phytochemical, Pharmacological, Morphological and Molecular Studies on the Poisonous Plant Thevetia Peruviana (Pers.) K. Schum P Yadav, SD Mishra Emerging Strategies in Research—Going Beyond Disciplinary Boundaries, 162 , 2023 2023
Examination of Alterations in Multi-Generation Photocopied Documents H Bansal, NMV Prasad, SD Mishra Industry 5.0 and Paradigm Shift—Emerging Challenges 2 (S3), 71 , 2023 2023
Dental Age Estimation using Kvaal’s Method in Indore Population: A Pilot Study S Tekade, SD Mishra Industry 5.0 and Paradigm Shift—Emerging Challenges, 98 , 2023 2023
Fingerprint analysis for the determination of hand origin (right/left) using the axis slant in whorl patterns N Kapoor, A Badiye, SD Mishra Forensic Sciences Research 7 (2), 285-289 , 2022 2022 Citations: 15
Single-digit fingerprint analysis for hand determination: a study of twinned loops N Kapoor, SD Mishra, A Badiye Medicine, Science and the Law 60 (3), 182-187 , 2020 2020 Citations: 11
Age estimation from tooth-pulp area ratio: A preliminary study N Kapoor, P Kothari, RK Shukla, SD Mishra, A Badiye La revue de médecine légale 11 (1), 11-14 , 2020 2020 Citations: 14
A novel approach for sex determination using palmar tri-radii: a pilot study A Badiye, N Kapoor, SD Mishra Journal of forensic and legal medicine 65, 22-26 , 2019 2019 Citations: 16
Handwriting Analysis: A Study of Personality Traits in Individuals of Different Professions H Bansal, A Badiye, N Kapoor, SD Mishra Innovation, Invention & Industralization: Emerging Issues, 33-35 , 2019 2019
Palmar fasciitis and polyarthritis as a paraneoplastic syndrome P Padhan, S Mishra Indian Journal of Rheumatology 13 (2), 129-130 , 2018 2018 Citations: 2
Occurrence of Carrion Feeding Flies in Nagpur City: A Preliminary Study A Badiye, N Kapoor, SD Mishra Innovation in Science, Technology and Management Challenges and … , 2018 2018
Revealing Transplanted Signatures with the Help of Photographic Image Processing Software: A Case Study AS Swati Dubey Mishra Global Journal Of Engineering Science And Researches, 377-380 , 2018 2018
Determination of Disulfiram in several Pharmaceutical Products using Thin Layer Chromatoghraphy VR Chourey, SD Mishra, A Maseeh Holkar Research Spectrum 2 , 2016 2016
Forensic Science, Technology and Society SD Mishra Science, Technology And Society, 519-521 , 2015 2015
MOST CITED SCHOLAR PUBLICATIONS
A novel approach for sex determination using palmar tri-radii: a pilot study A Badiye, N Kapoor, SD Mishra Journal of forensic and legal medicine 65, 22-26 , 2019 2019 Citations: 16
Fingerprint analysis for the determination of hand origin (right/left) using the axis slant in whorl patterns N Kapoor, A Badiye, SD Mishra Forensic Sciences Research 7 (2), 285-289 , 2022 2022 Citations: 15
Age estimation from tooth-pulp area ratio: A preliminary study N Kapoor, P Kothari, RK Shukla, SD Mishra, A Badiye La revue de médecine légale 11 (1), 11-14 , 2020 2020 Citations: 14
Monitoring strychnine and brucine in biochemical samples using direct injection micellar liquid chromatography SD Mishra, D Bose, SK Shukla, A Durgabanshi, J Esteve-Romero Analytical Methods 5 (7), 1747-1754 , 2013 2013 Citations: 14
Single-digit fingerprint analysis for hand determination: a study of twinned loops N Kapoor, SD Mishra, A Badiye Medicine, Science and the Law 60 (3), 182-187 , 2020 2020 Citations: 11
Determination of disulfiram by micellar liquid chromatography in illicit preparations SK Mourya, S Dubey, A Durgabanshi, S Kumar Shukla, J Esteve-Romero, ... Journal of AOAC International 94 (4), 1082-1088 , 2011 2011 Citations: 8
The impact of neurological disorders on handwriting: Implications for forensic document examination H Bansal, SD Mishra, M Singhal Wiley Interdisciplinary Reviews: Forensic Science 6 (6), e1536 , 2024 2024 Citations: 3
Simultaneous determination of three traditional and two novel Antiepilectic Drugs using Micellar Liquid Chromatography S Dubey, SK Shukla, A Durgbanshi, J Esteve-Romero, D Bose Int. J. Anal. Bioanal. Chem 3 (1), 1-8 , 2013 2013 Citations: 3
Novel Approach For Personal Identification Using Dorsal Knuckle Crease Patterns: A Pilot Study PS Biswas, SD Mishra Mapana Journal of Sciences 23 (4), 83 , 2024 2024 Citations: 2
Palmar fasciitis and polyarthritis as a paraneoplastic syndrome P Padhan, S Mishra Indian Journal of Rheumatology 13 (2), 129-130 , 2018 2018 Citations: 2
Rapid screening of alcohol deterrent drug ‘Disulfiram’using thin layer chromatography SK Mourya, S Dubey, D Bose, A Durgabanshi, P Monsanto Internet J Forensic Sci 3 , 2008 2008 Citations: 2
An assessment of biometrics tools: Fingerprint and knuckle crease patterns PS Biswas, SD Mishra Fostering Multidisciplinary Research for Sustainability, 145 , 2024 2024 Citations: 1
Determination of alpha-beta arteether in pharmaceutical products using direct injection micellar liquid chromatography S Dubey, D Bose, SK Shukla, A Durgbanshi, J Esteve-Romero, ... Journal of Liquid Chromatography & Related Technologies 37 (14), 1929-1939 , 2014 2014 Citations: 1
Dorso-palmar triangular finger flap-A new advancement flap for cover of finger amputations-A preliminary report S Mishra Indian Journal of Plastic Surgery 34 (02), 066-069 , 2001 2001 Citations: 1
Knuckle Crease Patterns in Forensic Biometrics: A Novel Trait for Identity SD Mishra Exploring the Intersection of Forensics and Biometrics, 133 , 2026 2026
Development of a Green and Robust Micellar Liquid Chromatography Method for the Simultaneous Determination of Malathion and Carbofuran. N Ingole, SD Mishra, D Bose Journal of Chemical Health Risks 16 (1), 2495 , 2026 2026
Knuckle Crease Patterns in Forensic Biometrics: A Novel Trait for Identity Verification and Investigative Applications PS Biswas, SD Mishra Exploring the Intersection of Forensics and Biometrics, 133-166 , 2026 2026
Analysis of Palmar Creases for Personal Identification A Beragi, SD Mishra Integrating Interdisciplinary Research for Societal Well-Being, 74 , 2025 2025
Phytochemical, Pharmacological, Morphological and Molecular Studies on the Poisonous Plant Thevetia Peruviana (Pers.) K. Schum P Yadav, SD Mishra Emerging Strategies in Research—Going Beyond Disciplinary Boundaries, 162 , 2023 2023
Examination of Alterations in Multi-Generation Photocopied Documents H Bansal, NMV Prasad, SD Mishra Industry 5.0 and Paradigm Shift—Emerging Challenges 2 (S3), 71 , 2023 2023
