Ajijolakewu Kamoldeen
@unilorin.edu.ng
Senior Lecturer/Department of Microbiology
University of Ilorin
Secondary School at Cardoso High School, Lagos
First Degree (BSc) at the University of Ilorin, Ilorin, Nigeria.
Second Degree (MSc) at the University of Ilorin, Ilorin, Nigeria.
PhD at the Universiti Sains Malaysia
EDUCATION
BSc; MSc and PhD Microbiology
RESEARCH, TEACHING, or OTHER INTERESTS
Biotechnology
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
Yusuf Opeyemi Oyebamiji, Kamoldeen Abiodun Ajijolakewu, and Ismail Abiola Adebayo
Springer International Publishing
Tariq Oluwakunmi Agbabiaka, Ismail Abiola Adebayo, Kamoldeen Abiodun Ajijolakewu, and Toyin Olayemi Agbabiaka
Elsevier
Azeez Omoniyi Adeoye, Ismail Abiola Adebayo, Adam Moyosore Afodun, and Kamoldeen Abiodun Ajijolakewu
Elsevier
Risikat Nike Ahmed, Mercy Oluwaseyi Bamigboye, Kamoldeen Abiodun Ajijolakewu, Sheriffdeen Olakunle Idris, and Nimat Toyosi Ajide Bamigboye
Univ. of Malaya
Antibiotics are one of the most exploited metabolites produced by soil actinomycetes. This study isolated fifteen actinomycetes (A1 – A15) from dumpsite soils within Ilorin metropolis & screened them for antibacterial activity. Isolates were identified with morphology & biochemical characteristics.Their activity against clinical bacteria (Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae & Staphylococcus aureus) was determined. Their bioactive metabolites after fermentation was assayed for by agar well diffusion & compared with reference antibiotics. Molecular analysis of two most active isolates was done. The most active metabolite was subjected to GCMS. All isolates were identified as Streptomyces, 7 isolates exhibited activity against 3 test bacteria with highest (10.5mm±0.35) by Streptomyces griseoplanus while S. aureus demonstrated total resistance to all isolates. Secondary activity revealed 7 of the metabolites as effective against the bacteria with highest activity (28.5±1.04mm) by S. sparsogenes showing similar activity with Cefuroxime (28.01±0.01mm). The two most active isolates were Streptomyces griseoplanus strain NR725RL-ISP 5009 & Streptomyces sparsogenes strain NBRC 1308616S & RNA nucleotide similarity revealed close phylogenic relationship. GC-MS revealed10 compounds in S. sparsogenes strain NBRC 1308616S metabolite. Streptomyces from dumpsite soils within Ilorin produced bioactive compounds against clinical test bacteria and thus could be potential sources of antibiotics.
Azeez Omoniyi Adeoye, Ismail Abiola Adebayo, and Kamoldeen Abiodun Ajijolakewu
Springer International Publishing
Muinat Olanike Kazeem, Kamoldeen A. Ajijolakewu, and Nor’ Aini Abdul Rahman
BioResources
Single cultures and co-cultures of Bacillus licheniformis and Bacillus paralicheniformis isolated from compost were evaluated for their carboxymethyl cellulase (CMCase) and filter paperase (FPase) production potential. Using a medium supplemented with microcrystalline cellulose (MCC), in the co-culture, CMCase and FPase activities increased 8.87- and 2.28-fold and 10.15- and 3.20-fold over B. licheniformis and B. paralicheniformis monocultures, respectively. The synergistic behavior of the two isolates might be due to the consumption of hydrolysis product (glucose, cellobiose) by one or both of the isolates, which improved their metabolic performance for cellulase secretion. Optimal conditions for cellulase production by this co-culture were a temperature of 45 °C, and pH 7 at 180 rpm in a medium containing rice bran at 1% (w/v) and chicken manure as nitrogen supplement at 2% (w/v). The maximum CMCase and FPase produced under the above conditions were 79.8 U/mL and 12.5 U/mL, respectively. This corresponds to 257.4- and 59.5-fold enhancement in CMCase and FPase activity, respectively, over B. licheniformis monoculture, and 306.9- and 83.3-fold increase with respect to the B. paralicheniformis monoculture. These results indicate that improved cellulase production can be achieved through co-culture and chicken manure nitrogen-supplement.
Risikat N. AHMED, Sesan M. SOBA, Mercy O. BAMIGBOYE, and Kamoldeen A. AJIJOLAKEWU
Society of Land Measurements and Cadastre from Transylvania
The present research aimed at screening various soils within Ilorin metropolis for antibiotic producing actinomycetes. The objectives of the study were to determine physiochemical parameters of soils, the occurrence of actinomycetes in soils, antibacterial potentials and identity of isolates. Soil parameters such as pH, temperature, moisture, organic matter and soil type were evaluated following standard procedures. Selective isolation to determine the occurrence of actinomycetes was performed by soil dilution using pour plate technique on starch casein agar. Preliminary antibacterial screening against 10 clinical test bacteria was performed using cross streak method. All isolates were initially identified based on morphological and biochemical characteristics, while the most bioactive isolates were further identified by molecular means. The soils were alkaline, with temperatures between 29 °C and 31 °C, moisture was in range of 0.72 ± 0.07c and 6.62 ± 0.42b. Highest organic matter content was 32.13 ± 0.20a with soil types mostly loamy and sandy loam. Ten actinomycetes (SM1 - SM10) were isolated, with the most frequently occurring isolate being SM3 and SM5 (16.7%). SM5 was the most active, inhibiting 9 out of 10 tests, with the highest inhibition against Staphylococcus aureus 25923 (24 mm ± 0.15a). All isolates were identified as Streptomyces by morphology and biochemical tests. Based on nucleotide similarity searches and phylogeny, two bioactive Streptomycetes were suggested as novel strains and thus named as Streptomyces bottropensis UIL RNA (SM5) and S. flavoviridis UIL RNA(SM7), which may serve as promising sources of antibiotics. Actinomycetes from Ilorin metropolis demonstrated broad spectrum of antibacterial activity against clinical test bacteria.
Rudi Dungani, Abdulwahab F. Owolabi, Chaturbhuj K. Saurabh, H. P. S. Abdul Khalil, Paridah M. Tahir, C. I. C. M. Hazwan, Kamoldeen A. Ajijolakewu, M. M. Masri, E. Rosamah, and P. Aditiawati
Springer Science and Business Media LLC
A. Kamoldeen Ajijolakewu, Cheu Peng Leh, Wan Nadiah Wan Abdullah, and Chee keong Lee
Elsevier BV
Kamoldeen A. Ajijolakewu, Cheu Peng Leh, Chee Keong Lee, and Wan Abdullah Wan Nadiah
Elsevier BV
Ajijolakewu A. Kamoldeen, Chee Keong Lee, Wan Nadiah Wan Abdullah, and Cheu Peng Leh
Elsevier BV
Kamoldeen Abiodun Ajijolakewu, Cheu Peng Leh, Wan Nadiah Wan AbduLLah, and Chee Keong Lee
BioResources
Effect of lignocellulosic medium supplemented with selected easily-metabolised carbon sources on microbial xylanase production was assessed. A newly isolated oil-palm-waste-domesticated bio-agent-producing fungus, identified based on rRNA analysis as T. asperellum USM SD4 was used as a representative organism. The potential of T. asperellum for enhanced xylanase production was evaluated by the statistical optimization of important cultural parameters via response surface methodology (RSM). T. asperellum showed optimum xylanase activity at pH 7; temperature 27 °C; moisture content 4 mL growth medium (gm): 1 gram dried substrate (gds) and inoculum size 2 x 106 spores/mL. Xylanase activity (2,337 IU/gds) attained in this study was far higher than ever reported for T. asperellum. Using the set of optimum conditions, the mixture of supplementary sugars to the lignocellulosic medium initiated xylanase repression in a concentration-independent manner. However, the degree of repression depended on the nature and type of respectively added sugar. The repressive effect exerted by monosaccharides (xylose, glucose, and fructose) was greater than exerted by either of dimeric (cellobiose and sucrose) or polymeric (xylan) sugars. Of all added substrates, xylan exerted the least repressive effect. Using xylose as a representative sugar, mechanism of xylanase repression was decisively explained and supported with experimental data.