@unilorin.edu.ng
Professor, Faculty of Veterinary Medicine
Assistant Vice Chancellor
University of Ilorin
Prof Ambali obtained the DVM, and M.Sc., and Ph.D. degrees in Veterinary Toxicology from the Ahmadu Bello University in 1992, 2004, and 2009, respectively. He is currently a Professor of Environmental Toxicology and the Deputy Vice-Chancellor, Management Services, and a member of the Governing Council of the University of Ilorin. He had served both Universities at the Departmental, Faculty, and University levels. He was the Head of the Department of Veterinary Physiology and Pharmacology, Sub-Dean, and Dean of the Faculty of Veterinary Medicine from 2012-2017. He was Chairman of the Technical Sub-committee of the Appointments and Promotions Committees from 2018-2020. He is a member and fellow of various academic, professional, and cultural organizations. With an h-index of 26 with over 130 articles in peer review journals and books, Prof. Ambali has attended many local and international conferences. He is married and blessed with children.
Doctor of Veterinary Medicine - Ahmadu Bello University, Zaria, Nigeria
M.Sc. Veterinary Toxicology, Ahmadu Bello University, Zaria, Nigeria
Ph.D. Veterinary Toxicology, Ahmadu Bello University, Zaria, Nigeria
Toxicology, Veterinary, Pharmacology, Toxicology and Pharmaceutics, Neuroscience
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Aishat Omobolanle Olatunji, Joseph Olusegun Ayo, Mohammed Musa Suleiman, Suleiman Folorunsho Ambali, Muftau Shittu, Ganiu Jimoh Akorede, Abdulfatai Aremu, Ibrahim Yusuf Lamidi, Basiru Afisu, and Olubukola Tolulope Adenubi
Springer Science and Business Media LLC
Muftau Shittu, Suleiman Folorunsho Ambali, Joseph Olusegun Ayo, Mohammed Umaru Kawu, Motunrayo Ganiyat Akande, and Zainab Abdulmalik
SAGE Publications
The ability of melatonin to reduce growth inadequacy induced by parental and nutritional combination administration to chlorpyriphos (Ch) and cypermethrin (Cy) was examined in male albino rats. Oral alimentation was given to gravid dams divided into six groups ( n = 10; age: 12 weeks) from the first day of pregnancy to the 21st postnatal day. Distilled water (DW), Soya oil (SYO), and melatonin (MeL) groups were exposed to 2 mL/kg, 2 mL/kg, and 0.5 mg/kg, respectively; the Ch+Cy group was co-exposed to Ch (1.9 mg/kg of LD50) and Cy (7.5 mg/kg of LD50); the MChCy group was preconditioned with MeL (0.5 mg/kg), followed by co-exposure to Ch and Cy; and the ChCyM group was exposed to Ch and Cy and post treated with MeL. Male offspring rats were tested for ontogeny criteria at various points after accouchement. MeL pre- and post-administration reduced the variation in litter size and weight, number of live/dead pups, anogenital distance , crown-rump length, the timing of eye and ear openings, and testicular descent caused by fetal and nutritional co-administration to Ch+Cy in offspring male albino rats. MeL demonstrated preventive promise as a result of its apparent antioxidative capability.
Ganiu Jimoh Akorede, , Suleiman Folorunsho Ambali, Aisha Omobolanle Olatunji, Abdulfatai Aremu, Akeem Olayiwola Ahmed, Afisu Basiru, Mistura Oyebisi Azeez, Fatima Sanusi, Rafiu Adebisi Kadir,et al.
Negah Scientific Publisher
Background: Drugs are the mainstay of the clinical management of epilepsy. Carbamazepine (CBZ) is commonly used for treating epilepsy and neuropathic pain. This drug has been reported to have toxic effects on the hematological system due to its induction of oxidative stress. This study aimed to investigate the protective effects of vitamin C against hematological and thyroid toxicities caused by the chronic use of carbamazepine in male Wistar rats. Methods: Thirty-two adult Wistar rats were categorized randomly into four groups of eight rats each and treated as follows: Group 1 received distilled water (2 mL/kg); group 2 was treated with vitamin C (100 mg/kg); group 3 received carbamazepine (20 mg/kg), and group 4 was pre-treated with vitamin C (100 mg/kg) and given carbamazepine (20 mg/kg) 30 min later. All treatments were administered via gavage once per day over fifteen consecutive weeks. The rats’ blood samples were tested for changes in hematological parameters while the sera were evaluated for liver biochemical enzymes and thyroid hormone levels. Results: The results revealed that pre-treatment with vitamin C protected against alterations in parameters associated with hematological and thyroid toxicities. Conclusion: Based on the study results, it was concluded that: a) The chronic use of CBZ caused hematological and thyroid toxicities, and b) Vitamin C protected against these toxicities. Therefore, it is highly likely that vitamin C has the potential to protect experimental animals against injuries induced by CBZ to the liver, blood cells, and hypothalamic-pituitary-thyroid axis in a Wistar rat model.
Zainab Abdulmalik, Muftau Shittu, Sani Adamu, Suleiman Folorunsho Ambali, and Bolaji Fatai Oyeyemi
Elsevier BV
Aishat O. Olatunji, Joseph O. Ayo, Mohammed M. Suleiman, Suleiman F. Ambali, Muftau Shittu, Ganiu J. Akorede, Lukman O. Raji, Jamila A. Atata, Khalid T. Biobaku, and Mistura O. Azeez
Springer Science and Business Media LLC
Ganiu Jimoh Akorede, Suleiman Folorunsho Ambali, Aisha Omobolanle Olatunji, Abdulfatai Aremu, Afisu Basiru, Akeem Olayiwola Ahmed, Mistura Olabisi Azeez, Isiaku Abdulmajeed, and Rafiu Adebisi Kadir
Elsevier BV
Muftau Shittu, Suleiman Folorunsho Ambali, Joseph Olusegun Ayo, Mohammed Umaru Kawu, and Aishat Omobolanle Olatunji
Springer Science and Business Media LLC
Ganiu Jimoh Akorede, Suleiman Folorunsho Ambali, Mikail Garba Hudu, Mohammed M. Suleiman, Kolawole Yusuf Suleiman, Halimat Amin Abdulrahim, Lukman Oladimeji Raji, and Isiaku AbdulMajeed
Springer Science and Business Media LLC
O. B. Daodu, H. O. Jegede, J. O. Aiyedun, O. O. Oludairo, I. D. Olorunshola, O. C. Daodu, A. Ajadi, and S. F. Ambali
Springer Science and Business Media LLC
Emmanuel Vandi Tizhe, Najume Dogon-Giginya Ibrahim, Mohammed Yakasai Fatihu, Suleiman Folorunsho Ambali, Ikechukwu Onyebuchi Igbokwe, and Ussa Delia Tizhe
SAGE Publications
Objectives To assess the effects of zinc pretreatment on hepatorenal toxicity following chronic exposure to glyphosate-based herbicides in male rats. Methods Following zinc pretreatment (50 mg/kg and 100 mg/kg), 14.4 to 750 mg/kg of oral glyphosate (Bushfire® herbicide) was administered daily for 36 weeks. Thereafter, serum samples were obtained following jugular venipuncture. Liver and kidney samples were processed for histopathological examination. Results Serum aspartate aminotransferase, alanine aminotransferase, and alkaline phosphatase activity as well as levels of bicarbonate, calcium, creatinine were significantly increased following chronic exposure to Bushfire®. Serum levels of sodium, potassium, chloride, total protein, albumin, globulin and urea were unchanged. Moderate to severe coagulative necrosis of hepatocytes as well as glomerular and renal tubular necrosis were observed in herbicide-treated rats. Zinc pretreatment reduced the elevation of serum enzymes associated with hepatobiliary lesions, abrogated hypercalcemia and metabolic alkalosis, and mitigated serum accumulation of creatinine following Bushfire® exposure, but was ineffective in completely preventing histological lesions. Conclusion Chronic Bushfire® exposure in rats caused hepatorenal toxicity. The effects of exposure on serum parameters were ameliorated by zinc pretreatment, but the histopathological changes associated with toxicity persisted in milder forms in zinc-pretreated animals.
Emmanuel Vandi Tizhe, Najume Dogon-Giginya Ibrahim, Mohammed Yakasai Fatihu, Suleiman Folorunsho Ambali, Ikechukwu Onyebuchi Igbokwe, and Delia Ussa Tizhe
Springer Science and Business Media LLC
Chidiebere Uchendu, S. F. Ambali, J. O. Ayo, and K. A. N. Esievo
Springer Science and Business Media LLC
Emmanuel Tizhe, Najume Ibrahim, Mohammed Fatihu, Suleiman Ambali, Ikechukwu Igbokwe, and Ussa Tizhe
SAGE Publications
Objectives To assess the toxicopathologic effects of chronic exposure to the glyphosate-based herbicide Bushfire® on the pancreas of Wistar rats and the protective role of zinc. Methods We exposed the rats to daily doses of 14.4 to 750 mg/kg body weight of the glyphosate-based herbicide Bushfire® and to 50 or 100 mg/kg zinc, and measured blood glucose levels and serum insulin levels. Tissue samples were evaluated for histopathological alterations. Results Levels of both blood glucose and serum insulin increased in glyphosate-exposed rats, and moderate to severe degenerative changes were observed in both glandular pancreatic acinar cells and islets of Langerhans in all rats exposed to glyphosate. These effects were prevented by pretreatment with zinc. Conclusion Chronic exposure to glyphosate can alter pancreatic function and histoarchitecture, but zinc supplementation can mitigate these toxicopathologic effects.
Chidiebere Uchendu, Suleiman Folorunsho Ambali, Joseph Olusegun Ayo, and King Akpofure Nelson Esievo
Springer Science and Business Media LLC
Enokela Shaibu Idoga, Suleiman Folorunsho Ambali, Joseph Olusegun Ayo, and Aliyu Mohammed
Springer Science and Business Media LLC
Olusola Olalekan Oladipo, Joseph Olusegun Ayo, Suleiman Folorunsho Ambali, Bisalla Mohammed, and Tanang Aluwong
Elsevier BV
Chidiebere Uchendu, Suleiman F Ambali, Joseph O Ayo, and King AN Esievo
SAGE Publications
The study was aimed at evaluating the protective role of α-lipoic acid (ALA) on long-term exposure of rats to the combination of chlorpyrifos (CPF) and deltamethrin (DLT). Forty-two (42) male Wistar rats were divided into 6 exposure groups with 7 animals in each group: (I) soya oil (2 ml kg−1), (II) ALA (60 mg kg−1), (III) DLT (6.25 mg kg−1), (IV) CPF (4.75 mg kg−1), (V) (CPF + DLT) DLT (6.25 mg kg−1) and CPF (4.75 mg kg−1; 1/20th of the previously determined median lethal dose) and (VI) (ALA + CPF + DLT) pretreated with ALA (60 mg kg−1) and then co-exposed to CPF and DLT, 45 min later. The regimens were administered by gavage once daily for a period of 16 weeks. Sera obtained from blood collected at the end of the experimental period were used for the evaluation of serum glucose, total protein, albumin, urea, creatinine and the activities of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, lactate dehydrogenase and acetylcholinesterase. The liver homogenate was used to assay for the activities of superoxide dismutase and glutathione peroxidase and the concentrations of malondialdehyde, cytokine and tumour necrotic factor α. The result showed that the combination of CPF and DLT resulted in marked alterations of these biochemical parameters in most cases compared to either of the pesticides singly, supplementation with ALA ameliorated these alterations.
Olusola Olalekan Oladipo, Joseph Olusegun. Ayo, Suleiman Folorunsho Ambali, and Bisalla Mohammed
Informa UK Limited
Abstract The study aims to evaluate effects of chronic low-dose coexposure to lead (Pb), cadmium (Cd) and manganese (Mn) on hepatorenal toxicity and oxidative stress. Young male Wistar rats were treated with Pb acetate (1.4 mg/kg BW), Cd chloride (0.01 mg/kg BW), Mn chloride (0.14 mg/kg BW) and their combination (Pb + Cd + Mn) by oral gavage, for 15 weeks. Liver enzymes, albumin (Alb), globulin (Glb), total protein, creatinine, urea and electrolyte concentrations were measured in the serum. Hepatic and renal malondialdehyde (MDA), glutathione peroxidase-1 (GPx1) and metallothionein-1 (MT1) concentrations were measured by enzyme immunoassay technique. Chronic exposure to the metals significantly (p < .05) increased serum Glb concentration and decreased Alb/Glb ratio, compared to the controls. Serum creatinine concentration significantly (p < .05) decreased in the Pb, Cd and Pb + Cd + Mn groups, but elevated in the Mn group. Hepatic MDAs rose significantly (p < .05) in the Pb group, while hepatic GPx1 activities increased significantly (p < .05) in the Cd, Mn and Pb + Cd + Mn groups. Hepatic and renal MT1 concentration decreased (p < .05) in the Mn group only. Biochemical alterations were confirmed by light microscopy of the liver and kidneys, which showed degenerative changes. It is concluded that prolonged coexposure to environmentally relevant levels of Pb, Cd and Mn impairs liver and kidney functions via the induction of oxidative stress, and it underlines the importance of studying toxicants in combination.
Motunrayo G Akande, Yusuf O Aliu, Suleiman F Ambali, and Joseph O Ayo
SAGE Publications
The aim of this study was to investigate the effects of taurine (TA) on serum lipid profiles following chronic coadministration of chlorpyrifos (CP) and lead acetate (Pb) in male Wistar rats. Fifty rats randomly distributed into five groups served as subjects. Distilled water (DW) was given to DW group, while soya oil (SO; 1 mL kg−1) was given to SO group. The TA group was treated with TA (50 mg kg−1). The CP + Pb group was administered sequentially with CP (4.25 mg kg−1; 1/20th median lethal dose (LD50)) and Pb at 233.25 mg kg−1(1/20th LD50), while the TA + CP + Pb group received TA (50 mg kg−1), CP (4.25 mg kg−1), and Pb (233.25 mg kg−1) sequentially. The treatments were administered once daily by oral gavage for 16 weeks. The rats were euthanised, and the blood samples were collected at the termination of the study. Sera obtained from the blood samples were analyzed for total cholesterol, high-density lipoprotein cholesterol, triglycerides, and malondialdehyde, and also the activities of serum antioxidant enzymes including superoxide dismutase, catalase and glutathione peroxidase were analyzed. The low-density lipoprotein cholesterol, very-low-density lipoprotein cholesterol, and atherogenic index were calculated. The results showed that CP and Pb induced alterations in the serum lipid profiles and evoked oxidative stress. TA alleviated the disruptions in the serum lipid profiles of the rats partially by mitigating oxidative stress. It was concluded that TA may be used for prophylaxis against serum lipid disorders in animals that were constantly co-exposed to CP and Pb in the environment.
Folashade OLAIFA, Joseph Olusegun AYO, Suleiman Folorunsho AMBALI, and Peter Ibrahim REKWOT
Japanese Society of Veterinary Science
Experiments were performed to investigate the effect of ascorbic acid (AA) in reducing hemato-biochemical changes in pack donkeys during the cold-dry (harmattan) season. Six experimental donkeys administered orally AA (200 mg/kg) and six control donkeys not administered ascorbic acid were subjected to packing. Blood samples were collected from all donkeys for hematological and biochemical analyses. In the control donkeys, packed cell volume (PCV), erythrocyte count and hemoglobin concentration (Hb) decreased significantly (P<0.05) at the end of packing. In the experimental donkeys, there was no significant difference between the pre- and post-packing values of PCV, erythrocyte count and Hb. In the control donkeys, the neutrophil and neutrophil:lymphocyte ratio increased significantly (P<0.05) post packing, but in the experimental donkeys, the pre- and post-packing values were not significantly different. The eosinophil count increased significantly (P<0.05) in experimental and control donkeys post packing. In conclusion, packing exerted significant adverse effects on the hematological parameters ameliorated by AA administration. AA may modulate neutrophilia and induce a considerable alteration of erythroid markers in donkeys subjected to packing during the harmattan season.
S. Abalaka, M. Fatihu, N. Ibrahim, and S. Ambali
The Brazilian Society of Anatomy (SBA)
Abstract Introduction: Histopathological effects of ethanol extract of Adenium obesum stem bark on the liver of Clariasgariepinus was investigated over a 96-h exposure period as an effective organic piscicide. Materials and Methods: An acute static toxicity bioassay was performed after a preliminary concentration range finding test was conducted. Results: Exposed fish showed adaptation behavioural changes with those of respiratory distress and abnormal nervous signs of toxicity, which was concentration-dependent. Mortality was recorded in some of the exposed fish while a median lethal concentration of 7.15 mgL-1 was established for the extract in the exposed fish. Non-significant (p>0.05) changes were recorded in serum aspartate aminotransferase, alanine aminotransferase and alkaline phosphatase activities of the exposed ish. Moderate liver damage was observed in the exposed fish based on the degree of tissue change (DTC) grading where significant (p<0.05) differences were observed between the DTC of the exposed groups and the control. Conclusion: Ethanol extract of A. obesum stem bark was toxic to the exposed fish with evidence of hepatotoxicity and therefore, can be used to eradicate the hardy C.gariepinus from aquatic environments.
Samson Eneojo Abalaka, Muhammad Yakasai Fatihu, Najume Doguwar Giginya Ibrahim, and Suleiman Folorunsho Ambali
Elsevier BV
SE Abalaka, MY Fatihu, NDG Ibrahim, and SF Ambali
African Journals Online (AJOL)
Purpose : To investigate the acute toxicity of ethanol extract of Adenium obesum (Forssk.) Roem. & Schult stem bark in Wistar rats in relation to haematological parameters. Methods : This involved the administration of single dose of 300 mgkg -1 , 2000 mgkg -1 and 5000 mgkg -1 of the extract by oral gavage separately to three different groups of female rats (n = 3) one after another based on the absence of mortality and/or morbidity during a 14-day observation period. The control group was administered distilled water as placebo (1 mL per 100 g body weight). Results : The exposed rats did not show any obvious signs of toxicity, morbidity or mortality. Median lethal dose (LD50) of the extract was ≥ 5000 mgkg -1 or ∞ (unclassified) based on the fixed LD50 cut-off values. Final body weight of control rats (196.00 ± 3.06 g) was significantly (p 0.05). Packed cell volume, red blood cell count and haemoglobin concentrations in the rats (42.67 ± 1.33 %, 5.10 ± 0.20 x 10 12 L -1 and 130.70 ± 2.96 gL -1 , respectively did not change significantly (p > 0.05). However, the white blood cell count significantly increased from 7.50 ± 0.63 x 10 9 to 11.63 ± 0.50 x 10 9 L -1 while the lymphocyte count significantly increased from 5.81 ± 0.43 x 10 9 to 9.99 ± 0.42 x 10 9 L -1 (p < 0.05) at the highest extract dose (5000 mgkg -1 ) compared to their respective controls. Conclusion : Adenium obesum might not be haematotoxic and is considered a safe medicinal plant administered orally. Keywords : Adenium obesum , Haemoglobin, Blood count, Mortality, Morbidity, Haematotoxicity
Emmanuel Vandi Tizhe, Najume Dogon-Giginya Ibrahim, Mohammed Yakasai Fatihu, Igbokwe Ikechukwu Onyebuchi, Butcher Danladi Jonathan George, Suleiman Folorunsho Ambali, and Joshua Mallum Shallangwa
Springer Science and Business Media LLC
Shittu
Science Publications
Studies have shown that Chlorpyrifos (CPF), an Organophosphate (OP) insecticide alters both sex and thyroid hormones. Apart from inhibiting Acetylcholinesterase (AChE) activity, CPF has been shown to cause oxidative stress. The antioxidant potentials of many flavonoid-containing plants are increasingly being exploited in the therapy of many infectious and non-infectious diseases. Hibiscus Sabdariffa (HS) is one of the most widely used nutraceuticals that has been used traditionally to combat various illnesses due to its high flavonoid contents. The present study was therefore aimed at evaluating the ameliorative potentials of HS on subchronic chlorpyrifos-evoked alterations in sex and thyroid hormones in male Wistar rats. Forty-two (42) young adult male Wistar rats were divided at random into six groups containing seven (7) rats per group. Group I was administered distilled water (2 mL kg-1) only while group II received soya oil (2 mL kg-1), Group III was dosed with only aqueous extract of HS (500 mL kg-1 ~ 1/10th of the LD50), while group IV was given CPF (10.6 mL kg-1 ~ 1/8th of the LD50). Group V was pretreated with low dose of HS (250 mg kg-1 ~ 1/20th of the LD50) and then administered reconstituted CPF (10.6 mg kg-1), 30 min later. Group VI was pretreated with high dose of the HS (500 mg kg-1) and then administered CPF (10.6 mg kg-1), 30 min later. The regimens were administered orally by gavage once daily for a period of 11 weeks. At the end of the treatment period, sera obtained from the blood samples were analyzed for the levels of Follicle-Stimulating Hormone (FSH), Luteinizing Hormone (LH), testosterone, thyroid hormones (T3, T4 and TSH) and AChE. Thyroid and pituitary glands of each rat were also evaluated for Malondialdehyde (MDA) concentration. Results showed a non-significant (p>0.05) decrease in the concentrations of FSH, LH and testosterone in the CPF group relative to the other groups. There was significant decrease (p<0.05) in the concentrations of T3, T4 and an increase in TSH in the CPF group relative to the other groups. There were significant increases (p<0.05) in MDA concentrations in the thyroid and pituitary glands in the CPF group compared to the other groups. Pretreatment with aqueous extract of HS demonstrated a dose-dependent amelioration of CPF-induced alterations in the levels of testosterone, LH, FSH, AChE, T3, T4 and TSH in the serum and that of pituitary and thyroid glands MDA. This may be partly due to its high level of polyphenolic compounds that confer its antioxidant and possibly AChE restoration activities. It is therefore concluded that pretreatment of individuals who are occupationally exposed to CPF and probably other OPs with the extract of HS may result in protection from the insecticide-induced adverse reproductive health outcomes.