Rıza BİNZET
@mersin.edu.tr
Science and Arts Faculties
Mersin University
RESEARCH INTERESTS
Plant Systematics, Plant Anatomy, Palynology
Scopus Publications
Scopus Publications
Sanja Ćavar Zeljkovıć, Saliha Seyma Sahinler, Cengiz Sarikurkcu, Bulent Kirkan, Riza Binzet, and Petr Tarkowski
Springer Science and Business Media LLC
AbstractOnosma riedliana Binzet & Orcan, a traditionally used plant species, has been explored for its therapeutic potential in this study. The work presented here is the first report on the phenolic profile and biological activity of this species. Three extracts of varying polarity were prepared, with the methanolic extract containing the highest phenolic content (97.62 ± 0.20 mgGAE/g). Key phenolic compounds identified included pinoresinol, hesperidin, 4-hydroxybenzoic acid, and p-coumaric acid. The methanolic extract exhibited exceptional antioxidant properties, rivaling Trolox as a positive control, primarily attributed to hesperidin and luteolin. Moreover, the ethyl acetate extract demonstrated remarkable inhibition of cholinesterase and tyrosinase enzymes, while the methanolic extract displayed potent activity against carbohydrate hydrolytic enzymes, α-amylase and α-glucosidase. Again, phenolic compounds were shown to be responsible for the inhibition of cholinesterases and tyrosinase, but not for α-amylase and α-glucosidase. These findings underscore Onosma riedliana’s potential for incorporation into diverse pharmaceutical formulations, given its multifaceted bioactivity.
Metin Yıldırım, Gun Binzet, Rıza Binzet, and Erdal Yabalak
Informa UK Limited
Onosma sintenisii Hausskn. ex Bornm. (O. sintenisii) belongs to the Boraginaceae family and it is an endemic species from Irano-turanian phytogeographical region (central and eastern Anatolia) that distributes in steppe areas. This study aimed to investigate the chemical composition, antioxidant, in vitro cytotoxic and apoptosis induction of methanol extract of aerial parts of O. sintenisii. As a result of GC/MS analysis, 14 components were identified, and the major compounds of the extracts are retronecine (13.94%), α.-D-Glucopyranosiduronic acid (10.86%), melaniline (7.5%) and 1,2-Butanediol (4.02%), respectively. Antioxidant properties of O. sintenisii were determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, ferric reducing antioxidant power (FRAP) and superoxide radical scavenging activity methods. While the DPPH free radical scavenging activity results of O. sintenisii extract varied between 62.49% and 32.27%, reducing power activity and superoxide radical scavenging activity were found to be low. The result of the MTT assay revealed strong anticancer activity of O. sintenisii extract. The most significant cytotoxic effect was noted at a concentration of 1000 µg/mL after 48 hours. These findings together with flow cytometry analysis suggest that apoptosis can be the main mechanism underlying cell death after O. sintenisii extract treatment.
Ersan Turunc, Oskay Kahraman, Aylin Dogen, and Riza Binzet
Elsevier BV
Neva Ünal, Oskay Kahraman, Aylin Dögen, and Riza Binzet
Springer Science and Business Media LLC
Oskay Kahraman, Ersan Turunc, Aylin Dogen, and Riza Binzet
Springer Science and Business Media LLC
B. Kırkan, Cengiz Sarikurkcu, Riza Binzet and B. Tepe
AMG Transcend Association
Phytochemicals have significant biological effects. This study aimed to investigate the chemical composition, antioxidant and inhibitory activities of methanol (MeOH) extracts obtained from the aerial parts of Onosma microcarpum DC. and O. nana DC. on enzymes that play a critical role in digestive and cholinergic systems and melanogenesis process. The chemical compositions of the extracts were determined by using spectrophotometric and chromatographic methods. The biological activities of the extracts were determined by using antioxidant and enzyme inhibitory test systems. According to spectrophotometric analysis, both phenolic and flavonoid concentrations of O. nana were found higher than O. microcarpum (44.63 mg GAEs/g and 27.86 mg QEs/g, respectively). Chromatographic analysis shows that O. nana contains a high amount of hesperidin (53412.37 µg/g). In comparison, O. microcarpum has rosmarinic acid (13181.91 µg/g), apigenin 7-glucoside (11693.97 µg/g), luteolin 7 -glucoside (8632.03 µg/g) and pinoresinol (1014.26 µg/g) as the main compounds. In the ferrous ion chelating activity test, extracts exhibited almost similar activities. In contrast, radical scavenging (DPPH and ABTS), reducing power (CUPRAC and FRAP), and phosphomolybdenum tests resulted in the superiority of O. nana (229.98, 243.58, 327.46, 189.69, and 783.14, mg TEs/g, respectively). In the α-amylase inhibitory activity test, O. microcarpum showed higher activity (406.31 mg ACEs/g). In comparison, α-glucosidase (958.23 mg ACEs/g) and acetylcholinesterase (AChE) (2.80 mg GALAEs/g), butyrylcholinesterase (BChE) (2.59 mg GALAEs/g), and tyrosinase (200.43 mg KAEs/g) inhibitory activity of O. nana was found to be stronger than the other sample. (4) Conclusions: The chemical compositions and biological activities of the mentioned Onosma species were brought to the literature for the first time with this study. It is thought that O. nana can be an alternative source of phytochemicals in the food, pharmaceutical, and cosmetic industries due to its biological activity potential.
SERTAN ÇEVİK, OĞUZHAN KURT, AYŞİN GÜZEL DEĞER, Ferit KOCAÇINAR, and Rıza BİNZET
The Scientific and Technological Research Council of Turkey (TUBITAK-ULAKBIM) - DIGITAL COMMONS JOURNALS
Pelin Eroglu, Mehmet Ulas Civaner, Selda Dogan Calhan, Mahmut Ulger, and Riza Binzet
Slovenian Chemical Society
The Onosma L. (Lithospermae, Boraginaceae) genus contains many plant species with therapeutic properties due to its rich phytochemicals. Onosma mutabilis Boiss. & Hausskn. ex Boiss. (O. mutabilis) is the species for which there is not enough information on its characteristics. Objective: The total phenolic content, antioxidant activity, possible bioactive compounds, and antibacterial activities of ethanolic extracts of leaf, stem, root, and flower parts of endemic O. mutabilis were investigated. Conclusions: The total phenolic content of all O. mutabilis extracts was in the range of 9.2–31 mg gallic acid equivalent (GAE)/g of extract. According to the results of antioxidant activity, the IC50 antioxidant capacity values determined by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method were between 4.39–29 μg/mL, while the equivalent trolox antioxidant activity determined by the cupric reducing antioxidant values (CUPRAC) was 0.45–0.78 mmol of trolox equivalents (TE)/g of extract. Bioactive compounds have been analysed using gas chromatography coupled with mass spectrometry (GC/MS) and were found to contain 29 different chemical components. All plant extracts tested showed effective antibacterial activity against A. baumannii (ATCC 02026) (62.5 μg/mL MIC value) when compared to the reference drug Ampicillin (125 μg/mL).
Mehmet Firat and Rıza Binzet
Museum National d'Histoire Naturelle, Paris, France
ABSTRACT A new species of Onosma L. (Boraginaceae: Lithospermeae), from the Hakkari province of eastern Turkey, is described and illustrated. The new species is endemic and distributed in a narrow area in the Sat Mountains. Taxonomically the closest relative of Onosma satensis sp. nov. is O. polioxantha Rech. f. which also occurs in Turkey. In addition, it is primarily distinguished from O. polioxantha by its navicular leaves, longer and reticulate cauline leaves, longer pedicels and calyx, longer and densely patent short setulose outside, reticulate corolla and bigger nutlets. The geographical distributions and map, detailed photographs, habitat description, vernacular name, an IUCN conservation status and identification key for O. satensis sp. nov. and O. polioxantha were also provided. Nutlets and pollen micrographs of O. satensis sp. nov. are presented using scanning electron microscopy.
Ersan Turunc, Oskay Kahraman, and Riza Binzet
Elsevier BV
In the present study, a simple, cheaply and environmental friendly method was evaluated for the synthesis of silver nanoparticle via Cupressus sempervirens L. (CSPE) pollen extract as reducing and stabilizing agent. Various parameters such as volume of CSPE, temperature and reaction time on AgNPs formation were investigated spectrophotometrically to optimize reaction conditions. The electrochemical behavior of the biosynthesized AgNPs were investigated by cyclic voltammetry and square wave voltammetry techniques. An electrosensor based on AgNPs modified glassy carbon electrode were constructed and tested on electro reduction of hydrogen peroxide in phosphate buffer medium. The prepared electrosensor could detect the H2O2 in the range of 5.0 μM - 2.5 mM with a detection limit of 0.23 μM. In addition, the antioxidant activity of biosynthesized AgNPs were evaluated against DPPH free radical. Results obtained from the antioxidant study suggested that CSPE mediated AgNPs exhibit a good antioxidant effect.
Huseyin Suna, Macide Burcu Timur, Sertan Cevik, and Riza Binzet
Pakistan Journal of Botany
In this study, the anatomical and palynological characters of Onosma polyantha DC. and Onosma mitis Boiss. & Heldr. endemic to Turkey was investigated. To determine the anatomical characteristics, transverse sections of the root, stem and leaves, as well as adaxial and abaxial sections were taken from the leaves. It was determined that both species had a thick periderm tissue at the outermost part of the root. The endodermis was clearly observed and the pith was composed of tracheal elements. In the cross-section of the stem, only setose hairs were observed in O. polyantha, whereas in O. mitis, both setose hairs and rarely glandular hairs were present. The stomata were found to be at the same level as the epidermis in the O. mitis, while they were ± the upper level of the epidermis in O. polyantha. Two types of stomata (anomocytic and anisocytic) were seen on the lower and upper surfaces of the leaves, the density of the stomata was also higher on the lower surface of leaves in both species. In contrast, the stoma index was higher on the upper leaf surfaces than that of the lower side. The leaves were dorsiventral (=bifacial) in O. mitis and equifacial (=izobilateral) in O. polyantha. The pollen of both taxa were heteropolar, O. polyantha pollen grains were trisyncolporate at the distal pole, while in O. mitis pollen grains were trisyncolporate and tetrasyncolporate. Apocolpium was observed in the proximal poles of the trisyncolporate pollen, whereas it was not observed in the tetracolporate pollen of O. mitis. The pollen shape was spheroidal P/E=1.12 in O. polyantha and subprolata P/E=1.15 in O. mitis. In both species, the sculpture was scabrate (=granulate) and scabras were distributed on the surface of the pollen homogenously. The nutlet morphology of O. polyantha and O. mitis were also examined the nutlet ornamentations were reticulate, rugose, and reticulate-rugose, respectively; and epidermal cells were seen in different sizes. The aim of this study is to determine the anatomical and palynological characteristics of O. mitis and O. polyantha and to provide a more reliable diagnosis with the help of these characters.
Oznur Ergen Akcin and Riza Binzet
Bangladesh Journals Online (JOL)
Three Onosma L. species (O. papillosa Riedl, O. rutila Hub-Mor. and O. auriculata Aucher ex DC.) were examined anatomically. All these taxa had secondary root structure and xylem which were composed of sclerenchymatic cells and tracheary elements. O. papillosa has crystals and sclereids in the stem and leaf. Sand crystals are seen in the pith region of O. auriculata. O. papillosa and O. auriculata has bifacial leaf types and O. rutila has equifacial type. Stomata are anisocytic, anomocytic and staurocytic. These species have long and short simple eglandular and glandular trichomes. Setae with glabrous tubercles are present in O. papillosa and O. rutila. O. auriculata has porrect-stellate trichomes. Simple trichomes are unicellular and generally short. They have lignified, ornamental or smooth walls.
Riza Binzet, Gun Binzet, Ilkay Gumus, Ersan Turunc, Ummuhan Solmaz, Ebru Keskin, Aylin Dogen, and Hakan Arslan
Informa UK Limited
Abstract: The present study reports the chemical composition and antimicrobial activity of Onosma sieheana roots. Essential oil (EO) and extracts were isolated from the roots of Onosma sieheana by hydrodistillation and Soxhlet extraction, respectively. Essential oil and methanol extract were further analyzed by using the Gas chromatography-Mass spectrometry (GC/MS) technique. The extraction yields were 6.50, 0.35, and 1.70 with methanol, petroleum ether, ethyl acetate, respectively, and 0.07% for essential oil. Exactly sixty-one compounds were identified in essential oil and twenty-three compounds were identified in methanol extract. The main component of the essential oil was p-cymene (27.32%), whereas the main component of the methanol extract was methyl palmitoleate (15.19%). Although, the essential oil extracted from the roots of Onosma sieheana by the hydrodistillation method, was characterized by a high level of sesquiterpenes, the one extracted from Onosma sieheana roots by the Soxhlet extraction method was characterized by a high level of monoterpenes. Subsequently, these extracts were investigated for their antibacterial activity against two Gram- positive and two Gram-negative strains as well as for their antifungal activity against two yeast strains. As a result of antimicrobial studies, it has been determined that essential oil of Onosma sieheana roots have more effective antifungal activity than antibacterial activity. The antimicrobial effects of the extracts were found to be similar.
Oskay Kahraman, Riza Binzet, Ersan Turunc, Aylin Dogen, and Hakan Arslan
IOP Publishing
RIZA BİNZET and ÖZKAN EREN
Magnolia Press
A new species of Onosma (Boraginaceae: Lithospermeae) from the Erzincan province of eastern Anatolia, O. erzincanica, is described and illustrated. It belongs to the Haplotricha informal group which comprises the species with basal leaves covered by simple setae only. The species appears to be endemic to scree and is very narrowly distributed. The closest relative of O. erzincanica is O. obtusifolia Hausskn. & Sint. ex Riedl, which also occurs in Turkey. In addition to the its restriction to screes, it is primarily distinguished from O. obtusifolia by its golden yellow, shorter (14–17 mm) and clavate corollas, longer pedicels (2–5 mm), shorter filaments (ca. 4 mm), acute cauline leaves and having stems covered not only with 2–5 mm long patent setae but also with short hairs, and chartaceous to membranous leaves, calyx and bracts when dried. The geographical distribution of O. erzincanica and O. obtusifolia are mapped and an identification key for those species is provided. Pollen and nutlet micrographs of O. erzincanica are presented using scanning electron microscopy. The site conditions and conservation status of O. erzincanica are also considered.
Ersan Turunc, Riza Binzet, Ilkay Gumus, Gun Binzet, and Hakan Arslan
Elsevier BV
Abstract Biosynthesis of silver and palladium nanoparticles was carried out by using Lithodora hispidula (Sm.) Griseb. Leaf extract as a reducing agent. The silver and palladium nanoparticles (NPs) were characterized by UV-Visible spectroscopy, inductively coupled Plasma-Mass Spectrometer, X-ray diffraction, scanning electron microscopy and energy-dispersive X-ray analysis. UV-Vis spectra of biosynthesized silver nanoparticles showed an absorption band at 425 nm. The formation of palladium nanoparticles was confirmed by the disappeared absorption band at 410 nm. Scanning electron microscopy analysis showed that Ag, AgPd spherical and Pd nanoparticle morphology were rod in shape. The electrochemical behaviour of Ag, Pd and AgPd alloy nanoparticles were investigated by cyclic voltammetry. Modified glassy carbon electrodes were constructed by using Ag, Pd and AgPd nanoparticles and used in electrocatalytic reduction of hydrogen peroxide in phosphate buffer medium (pH = 6.5), in potential range of 0.0 and -0.8 V vs . Ag/AgCl at 100 mV/s scan rate. The AgPdNPs-GCE based sensor could detect the H 2 O 2 in the linear range of 0.02–5.0 mM with a detection limit of 0.52 μM. The AgPdNPs-GCE showed a good repeatability and stability for detection of H 2 O 2 . Furthermore, it exhibited no interference with some biological species such as glucose, ascorbic acid and uric acid.
Rıza Binzet
Pensoft Publishers
Abstract Onosma anatolica Binzet, is described and illustrated as a new species from Niğde province in southern Anatolia, Turkey. It belongs to sect. Onosma L. subsect. Asterotricha (Boiss.) Gürke. The new species is closely related to Onosma subulifolia Riedl from which it is distinguished. Onosma anatolica is readily distinguished from Onosma subulifolia by its sterile shoots, the green-grey stem indumentum, longer bracts, yellow and puberulous petals. The IUCN threat category of Onosma anatolica is determined as “CR (Critically Endangered)”. A distribution map and anidentification key for Onosma anatolica and Onosma subulifolia supplement the study.
Rıza BİNZET
The Scientific and Technological Research Council of Turkey
Onosma malatyana Binzet is described and illustrated as a new species from Malatya and Tunceli provinces in eastern Anatolia, Turkey. The new species belongs to sect. Onosma L. Subsect. Asterotricha (Boiss.) Gurke. And is closely similar to O. Auriculata DC. And O. Gigantea Lam. Diagnostic morphological and palynological characters of these species are compared and discussed. The new species distinctly differs from the similar two species by its patent setose and short hair of stem indumentum; 60-85 × 20-40 mm, oblanceolate-obovate basal leaves; hairy corolla; and having 4.5 × 4 mm, obtuse-acuminate nutlets with ventral keel and horizontally incurved beak. The IUCN threat category of O. Malatyana is determined as "CR (Critically Endangered)". Finally, the geographical distribution of O. Malatyana and the other closely similar species are mapped and the identification key of those species is provided.
Nurcan Koleli, Aydeniz Demir, Cetin Kantar, Gunsu Altindisli Atag, Kadir Kusvuran, and Riza Binzet
Elsevier
Field and laboratory studies were performed to: (1) determine heavy metal concentration of serpentine soils in Mersin-Findikpinari, Turkey, (2) find possible metal hyperaccumulator plant species (ecotypes and cultivars) growing on the serpentine soils in the region, and (3) determine the effects of EDTA (ethylenediaminetetraaacetic acid) on the phytoextraction potential of selected plant / plants as metal hyperaccumulators. A total of 11 soil samples and 123 plant species (members of 23 genera and 15 families) from different sampling locations were collected during sampling periods covering a period of 24 months. Total and available Cr, Ni, Cu, Zn, Cd, Pb and Co concentrations in the collected soil and plant samples were analyzed by ICP-MS (inductively coupled plasma mass spectrometer). The results indicated that each soil contained a high concentration of one or more metals. The maximum concentrations of total metals in soils (as dry matter) were 909 mg Cr kg −1 , 3615 mg Ni kg −1 , 246 mg Cu kg −1 , 467 mg Zn kg −1 , 8.2 mg Cd kg −1 , 111 mg Pb kg −1 and 214 mg Co kg −1 . Among all plant species , Thlaspi elegans Boiss. (15,693 mg Ni kg −1 dry matter), Alyssum murale Waldst. & Kit. (13591 mg Ni kg −1 dry matter) showed the highest Ni concentration. A pot experiment was therefore conducted to examine the effect of adding EDTA to improve the phytoextraction of Ni from artificially contaminated soil by T. elegans which is an endemic Ni hyperaccumulator in the Mersin-Findikpinari (Turkey) serpentine soils. This is the first report on the effect of Ni hyperaccumulation in T. elegans exposed to different Ni and EDTA concentrations in pot experiments. T. elegans seeds were planted in pots with Ni concentrations ranging from 0 to 2000 mg kg −1 (0, 500, 1000, 1500 and 2000 mg Ni kg −1 ) in the absence or presence of 10 mg kg −1 EDTA. The plants showed a remarkable resistance to Ni toxicity, with no visual toxic symptoms including chlorosis and necrosis when exposed to high Ni concentrations throughout 60 days of growth. After 60 days of growth, the dry matter, Ni concentration and Ni content of plants were analyzed. In order to indicate the ability of plants to tolerate and accumulate Ni, we calculated the biological accumulation coefficient (BAC). The addition of 10 mg kg −1 EDTA significantly increased both plant growth as well as Ni uptake, compared to the control. Although the Ni uptake by T. elegans increased with increasing initial Ni concentrations, at higher concentrations a significant decrease was observed in the plant growth. The plant accumulated 13216 mg kg −1 Ni in the absence of EDTA, an addition of 10 mg kg −1 EDTA increased Ni uptake and accumulation to 16632 mg kg −1 Ni at Ni application dose of 2000 mg kg −1 . The maximum Ni content accumulated by the plant was 44831 μg per plant in the absence of EDTA, the addition of 10 mg kg −1 EDTA increased Ni uptake to 53740 μg per plant at an initial Ni application dose of 500 mg kg −1 . The results showed that T. elegans can be used for phytoextraction of Ni-contaminated soils and that EDTA induces phytoextraction in soils. Similar results were obtained for the BAC. The values were greater than 1, providing further evidence for the transport of Ni from Ni-contaminated soils to plant shoots. The results also showed that EDTA increased the solubility of Ni in soil due to the formation of less toxic and highly soluble Ni–EDTA complexes and can be a good chelator candidate for T. elegans used for the environmentally safe phytoextraction of Ni in soils.