Julius Olaniyi Aiyedun

@unilorin.edu.ng

Associate Professor, Faculty of Veterinary Medicine
Associate Professor, Faculty of Veterinary Medicine
university of ilorin

Julius Olaniyi Aiyedun


                        Download Compact PDF  
https://researchid.co/aiyedun

RESEARCH, TEACHING, or OTHER INTERESTS

Veterinary, Veterinary, Veterinary, Veterinary
22

Scopus Publications

Scopus Publications

  • MOLECULAR DETECTION OF INVA VIRULENCE AND FLOR RESISTANCE GENES IN SALMONELLA ENTERICA ISOLATED FROM HAWKED RTE ROASTED/SPICED MEAT (SUYA) IN ILORIN, NIGERIA
    OLADAPO OYEDEJI OLUDAIRO, FATHIAT ENIOLA FABIYI, AISHA ANUOLUWAPO IMAM, ADEDOTUN KABIRAT BALOGUN, BUKOLA ARINOLA RICHARDS, UDUAK AKPABIO, JULIUS OLANIYI AIYEDUN
    Assiut Veterinary Medical Journal Egypt, 2026
    Salmonella remains a significant public health concern, particularly in populations where ready-to-eat products, such as roasted/spiced meat (suya), are popular. The consumption of products contaminated with bacteria strains harbouring virulence and resistance genes may result in illnesses such as gastroenteritis, typhoid fever or septicaemia that are tough to control. The invA and florR genes code for cell proteins needed for invasion of the host epithelial cells and antimicrobial resistance to florfenicol (FFC)/chloramphenicol (CM), an antibiotic commonly used in veterinary practice, respectively. This study aimed to detect the presence of invA virulence and floR antimicrobial resistance genes in the Salmonella enterica isolated from 102 Ready to Eat (RTE) suya samples obtained from hawkers within Ilorin, Nigeria, using PCR. The Salmonella strains were sub-cultured, while pure colonies were inoculated into tryptone soya broth, incubated for 24 hours at 37 °C, and transported to the Bioformatics Laboratory, Ibadan, for PCR analysis. DNA was extracted from the isolates and amplified using primers targeting specific parts of the organism’s genome. The PCR products were run on agarose gel electrophoresis and visualized with the gel imagery device. All five (100%) isolates displayed the 284bp invA and 926bp floR genes’ bands. This study confirmed the presence of invasion and antimicrobial resistance genes in all Salmonella enterica from RTE spiced roasted meat obtained from the study area. There is a need for continued surveillance and control measures to prevent foodborne illnesses that may be due to contamination by virulent and antimicrobial-resistant Salmonella in food.
  • The Role of Laboratory Professionals in Preventing Contamination of Food by Antimicrobial-Resistant Microorganisms (AMRMs)
    O.O. Oludairo, J. O. Aiyedun, B.B. Oludairo, M. P. Oludairo, F.E. Fabiyi, A.A. Imam
    Journal of Applied Veterinary Sciences, 2025
    Antimicrobial-resistant-microorganism (AMRM)-infected or contaminated food that leads to livestock losses and disease/death in humans is estimated to put 31 million people at risk of food insecurity in Nigeria by the last quarter of 2024. This study aimed to explicate the roles of food-industry stakeholders/laboratorians in preventing food contamination and animal/human infection by AMRMs. The objectives were to determine what food/food system, food security, and stakeholders in the food industry are; investigate the occurrence of AMRM in food; and elucidate preventive measures to be implemented to avoid AMRM in food. A structured questionnaire was administered to stakeholders and baseline information from published literature/established international standards was used to achieve this purpose. The majority (88%) of the respondents were from the Veterinary Teaching Hospital, University of Ilorin; all (100%) participants from the laboratories stated they knew the meaning of food security, while 56% of respondents that were ≤25 years old stated that AMRM could contaminate food. Only 52% of university-educated respondents agreed they were stakeholders in the food industry while 60% were veterinarians/medical practitioners. Reported contamination of food by AMRMs from harvest to consumption, especially in low/middle-income countries (LMICs) like Nigeria was by antimicrobial-resistant Staphylococcus spp., Bacillus spp., Proteus spp., Salmonella spp., and Clostridium spp. This was largely enhanced by the unrestricted use of antimicrobial agents in food animals, especially as growth promoters, leading to the production of unwholesome food, outbreaks of zoonoses, loss of livestock, and human diseases that were foodborne. Compulsory antimicrobial susceptibility tests should be done for isolates from food/food products to determine their suitability for use and the presence of AMRM. The diffusion method of antimicrobial susceptibility testing (AST) should strictly follow the International Standard Organization (ISO) protocol, which includes using pure colonies, overnight culture of 0.5 McFarland turbidity, and interpreting zones of inhibition using the Clinical and Laboratory Standard Institute (CLSI) recommended breakpoints. Only certified safe and wholesome food/food products should be passed to the public for consumption. Universities and researchers should carry out more extension work outside establishments’ walls to disseminate research results in food-AMRM.
  • Seromolecular surveillance of rabbit haemorrhagic disease virus in Nigeria
    Oluwafemi Babatunde Daodu, Joseph Ojonugwa Shaibu, Emmanuel Ayopo Aderounmu, Taiwo Oluwole Jolaoso, Daniel Oladimeji Oluwayelu, Olatunde Babatunde Akanbi, Isaac Dayo Olorunshola, Julius Olaniyi Aiyedun, Oladapo Oyedeji Oludairo, Rosemary Ajuma Audu, Oluwakemi Christiana Daodu
    Tropical Animal Health and Production, 2023
  • Ecology and epidemiology of Salmonella spp. isolated from the environment and the roles played by wild animals in their maintenance
    Oladapo Oyedeji Oludairo, Jacob K. P. Kwaga, Junaid Kabir, Paul A. Abdu, Arya Gitanjali, Ann Perrets, Veronica Cibin, Antonia Anna Lettini, Julius O. Aiyedun
    International Journal of One Health, 2023
    Salmonella is a ubiquitous organism of public health importance that causes diarrhea and other systemic disease syndromes. The ecology and epidemiology of the organism in addition to the roles played by wild animals are important in understanding its disease. Relevant published peer-reviewed literature was obtained after imputing the study's keywords into the Google search engine. The publications were thereafter saved for the study. The study revealed the ecology of Salmonella is directly related to its epidemiology. These were found to be either positively or negatively influenced by the living and non-living parts of the environment. Free-ranging and captive wild animals can serve as asymptomatic carriers of Salmonella, therefore, help to maintain the cycle of the disease since wildlife serves as reservoir hosts to over 70% of emerging zoonotic diseases. Cockroaches transmit Salmonella through their feces, and body parts and when ingested by birds and animals. The statistically significant over 83% of Salmonella isolation in lizards suggests the reptile could be a source of Salmonella distribution. Snakes, foxes, badgers, rodents, and raccoons have been reported to have Salmonella as a natural component of their gut with the ability to shed the organism often. The high occurrence (>45%) of diverse Salmonella serovars coupled with the fact that some of these animals were handled, kept as pets and consumed by man portends these animals as potential sources of transmission of the organism and the disease. The etiology and epidemiology of Salmonella are overtly affected by several environmental factors which also determine their survival and maintenance. The roles played by wild animals in the relationship, transmission, growth or interaction within and between Salmonella spp., the occurrence, prevalence, and distribution of the organism help maintain the organism in the environment. An understanding of the roles played by the different parts of the environment and wild animals in the ecology and epidemiology of Salmonella can help make informed decisions on the prevention and control of the diseases it causes. This review aimed to investigate the relationship between ecology, epidemiology, and environment, including the roles played by wild animals in the maintenance of the organism and its disease.
  • Isolation and Antimicrobial Resistance Phenotype of Salmonella species from Ready-to-Eat Roasted Meat (Suya) in Ilorin, Kwara State, Nigeria
    Oladapo Oludairo, Balogun A.K., Daodu O.B., Aiyedun J.O.
    Journal of Applied Veterinary Sciences, 2022
    Salmonella are pathogenic bacteria that can contaminate food products during or after processing. They are among the most important food borne pathogens worldwide contaminating a wide range of animal products including meat products. Human illnesses due to this pathogen are attributed to poor biosecurity in production and improper processing/handling of meat and meat products. This is more likely where surveillance and regulatory control are weak. Ready-to-eat (RTE) foods, like roasted meat (Suya) in Ilorin, do not undergo any treatment to ensure its safety before consumption, therefore the risk of food borne disease occurrence must be considered if these pathogens are present in the food. This study aimed to evaluate the prevalence of Salmonella in Suya in Ilorin, Nigeria. A total of 102 Suya samples were collected and analysed for the presence of Salmonella. Samples were obtained from 5 major markets, consisting of a total of 15 Suya retail outlets. Organism isolation and identification were done using conventional methods. Confirmation of suspected isolates was done using Microbact GNB 24E. Five samples (4.9%) were positive for Salmonella. Confirmed Salmonella isolates were evaluated in vitro for susceptibilities to 12 commonly used antimicrobial agents. All (100%) of the confirmed Salmonella isolates exhibited multiple resistance to at least 3 antibiotics and exhibited five antimicrobial resistance phenotypic patterns. The classes of antibiotics they were resistant to are: aminoglycosides, cephalosporin, penicillin and tetracycline. The isolation of Multiple Drug Resistant (MDR) Salmonella species in this study makes Suya potential vehicle for the transmission of Salmonella. Effort should be made to sensitize producers of Suya on the potential risk of the product being vehicles for the transmission of food borne pathogens and the need to maintain high level hygiene during production. The public should be educated to only patronize reliable Suya joints. Constant research work should be carried out to evaluate the level of contamination of ready to eat foods like Suya in order to be able to take informed decisions.
  • An overview of Animal and Human Brucellosis in Nigeria
    J. Aiyedun, O. Oludairo, I. Olorunshola, B. Akanbi, J. Bale
    Journal of Applied Veterinary Sciences, 2022
    Brucellosis is a bacterial disease in humans and animals caused by a group of organisms in the genus Brucella. It is highly contagious and one of the most important zoonosis in the world. The disease remains endemic in Nigeria and its actual incidence and prevalence are unknown due to poor surveillance and inadequate disease reporting system. A comprehensive review of the literature published online was carried out on manuscripts published as far back as 1976 to 2021, A computerized search of existing literature was conducted using the Google search engine, PubMed electronic database and Commonwealth Agricultural Bureaux (CAB) abstracts to identify and review relevant publications on brucellosis in animals and humans in Nigeria using the following search terms: brucellosis, malta fever, undulant fever, febrile fever, Gibraltar fever, gastric fever, remittent fever, Mediterranean fever, bangs disease and contagious bovine/ovine abortion. Our review showed that brucellosis is widely distributed in Nigeria amongst human and animal hosts. Undulant fever is the most commonly reported syndrome in humans, while abortion is the most prevalent symptom documented in animals. Serological techniques like rose-Bengal/card test (RBT), standard agglutination tests (SAT), complement fixation test (CFT), indirect enzyme immunoassays (ELISA) and polymerase chain reaction (PCR) are often used to determine the prevalence of brucellosis in human and animal hosts. Losses enumerated in the literature include those due to abortions, diminished milk production and contamination of milk, mastitis, animal culls and condemnation of infected animals due to breeding failure and inability to participate in the international animal export trade. In humans, brucellosis reduced work capacity through the sickness of the affected people and can be acquired from animals. There is need for a drastic public health interventions and control measures on brucellosis in the livestock industry in Nigeria.
  • Assessment of the Knowledge, Attitude and Practices of Dog Owners on Rabies in Ilorin, Kwara State, Nigeria
    J. Aiyedun, A. Darajat, Daodu B.O., Oludairo O.O., I. Olorunshola, Nwoha R.I.O., I. Oluboyede
    Journal of Applied Veterinary Sciences, 2022
    Rabies is an acute viral zoonotic disease that affects all warm-blooded animals. Its constant evaluation of the perception of the public is essential. This study assessed the knowledge, attitude and practices of dog owners on rabies in Ilorin, Kwara State, Nigeria. This was achieved by administering 150 structured questionnaires to dog owners, breeders and hunters in the study area using face to face interviews. The data collected were analysed using SPSS version 21. Dog owners with more than 3 dogs (13.3%) and those with one dog (50.7%) had the highest (80%) and least (47.4%) rabies vaccination rates respectively. Dogs kept for both breeding and security (18%) were vaccinated more than those kept for (74.1%) other reasons. Lhasa apso and local breeds were the highest (100%) and least (14.3%) vaccinated dogs in the study. Dogs < 1 year (30.7%) and those >6 years (0.7%) recorded (65.2%) and (0%) current rabies vaccination status respectively, while 90% and 72.6% of the respondents knew about rabies and its transmission to man respectively, only 58.7% had updated rabies vaccination for their dogs. Although 56% of the dog owners in this study rightly indicated how rabies is transmitted, 16% and 64.7% didn’t know how humans contract the disease and the clinical presentations in dogs respectively. Most (35.6%) respondents that didn’t have up to date vaccinations for their dogs didn’t have cogent reasons for failing to do so. Dog owners that were not knowledgeable about pre-exposure rabies vaccination were 58.7% while only 7.3 % of them had received previous shots of the vaccine. Dog bite experience was recorded in 78% of the respondents while only 20.7% of the 82.7% that claimed to know what to do when bitten by a dog identified the right course of action. Most (69.3%) respondents however indicated they would seek medical help at the government hospital (40%) if bitten by a rabid dog. The result of this study showed that there is some knowledge gap in the knowledge of rabies among stakeholders in the study area. Periodic mass rabies awareness campaigns to improve the perception of the disease in the study area is indicated.
  • A review of the International Organization for Standardization (ISO) guidelines for the detection of Salmonella from faeces
    Oladapo Oludairo, Jacob Kwaga, Junaid Kabir, P. Abdu, Arya Gitanjali, Ann Perets, Veronica Cibin, A. Lettini, J. Aiyedun
    Journal of Applied Veterinary Sciences, 2022
    Adherence to the guidelines of The International Organization for Standardization (ISO) for the isolation of Salmonella from faeces is important to laboratory personnel, researchers and epidemiologists to ensure effective diagnosis, maximal recovery and high sensitivity/specificity of laboratory tests. The aim of this study is to review the ISO guidelines for the detection of Salmonella from faeces using standards published by ISO and other internationally recognized bodies. The ISO 6579 of 2002 described the process for the detection of Salmonella in faeces. About 25g of faecal samples is collected, mixed together and specimen taken from different parts to ensure maximum recovery of the organism. Processes for the identification of the organism were prescribed by the standard. In the amendment made to ISO 6579 (Annex D) it was recommended that Modified Semisolid Rappaport Vasiliadis (MSRV) be used as selective enrichment medium. Further amendment made to this standard was in 2007 where clause 4 described non-selective pre-enrichment, selective enrichment, use of 2 selective solid media and biochemical tests as the 4 stages of the isolation of Salmonella in faeces. The amendment of ISO 6579 in 2017 combined the guidelines for the isolation of Salmonella species, S. Typhi/Paratyphi from milk/milk-products and faeces. It proposed the use of selenite cysteine broth in addition to Rappaport Vasiliadis (RVS) and Muller-Kauffmann Tetrathionate-Novobiocin (MkTTn) and BSA in addition to Xylose Lysine Deoxycholate (XLD) Agar for the isolation of S. typhi and paratyphi. It recommended the performance of methyl-red, Simmon-citrate, H2S, motility and urease biochemical tests, deleted Vogues Proskaer test while indole and β-Galactosidase (ONPG) tests were made optional. This standard has evolved over the years to ensure efficient recovery of Salmonella from faeces based on real-time result and feedback received by the organization from laboratories all over the world.
  • Epidemiology and public health implications of parasitic contamination of fruits, vegetables, and water in Kwara Central, Nigeria
    S. Ola-Fadunsin, A. O. Adebanjo, D. A. Abdullah, K. Hussain, I. Sanda, Musa Rabiu, I. Ganiyu, N. Elelu, J. Aiyedun, O. Oludairo
    Annals of Parasitology, 2022
    Fruits and vegetables are sources of parasitic infections in humans. This study was designed to determine the epidemiology of parasitic contamination of fruits, vegetables, and water, as well as the perception of retailers, buyers, and consumers about its contamination in Kwara Central, Nigeria. A cross-sectional study design was employed for this study. A total of 160 fruits, 349 vegetables, and 51 water samples were randomly purchased/collected from thirty different markets. The samples were subjected to sedimentation and modified Ziehl-Neelsen staining techniques. All the types of fruits and vegetables sampled were contaminated with one or more parasites. Eleven different species of zoonotic parasites (6 protozoans and 5 helminths) were detected. Entamoeba coli (39.8%) and Strongyloides stercoralis (9.6%) were the most prevalent protozoan and helminth respectively. The prevalence of other protozoan parasites ranged between 21.3% (Entamoeba histolytica) and 2.3% (Balantidium coli), while that of helminths was between 5.7% (Ascaris lumbricoides) and 2.1% (Trichuris trichiura). Source of fruits and vegetables, source of manure for cultivation, the source of water for washing/wetting purpose, market type, washing of fruits and vegetables before display, the presence of children at home and in the market, and knowledge of parasitic contamination were the risk factors associated (P<0.05) with parasitic contamination of fruits and vegetables among sellers. There is a need to educate the general public on the possibilities of parasitic contamination of fruits and vegetables and its public health implications.
  • Detection and molecular characterization of a first isolate of rabbit haemorrhagic disease virus in Nigeria
    O. B. Daodu, J. O. Shaibu, A. B. Richards, E. B. Folaranmi, S. Adegoke, A. Ajadi, I. D. Olorunshola, O. B. Akanbi, A. A. Afolabi, O. C. Daodu, J. O. Aiyedun, O. O. Oludairo, N. I. Halleed, R. A. Audu, D. O. Oluwayelu
    Tropical Animal Health and Production, 2021
  • Disease and mortalities in selected zoological gardens in Nigeria
    Olatunde B. Akanbi, Henry O. Jegede, Mohammed Adam, Oladapo O. Oludairo, Julius O. Aiyedun, Amos G. Rimfa, James Ahmed, Israel J. Barde, Aminu B. Hanga, Abdullateef A. Ajadi, Jamila A. Atata, Victor O. Taiwo, Shodeinde V. O. Shoyinka
    Comparative Clinical Pathology, 2021
  • Modulatory effects of melatonin on erythrocyte indices in Arabian stallions following a 30-km sub-maximal exercise
    Adakole Sylvanus Adah, Deborah Arimie Adah, Charles Obiora Nwonuma, Julius Olaniyi Aiyedun
    Comparative Clinical Pathology, 2020
  • Surveillance for avian influenza virus in captive wild birds and indigenous chickens in Nigeria
    O. B. Daodu, H. O. Jegede, J. O. Aiyedun, O. O. Oludairo, I. D. Olorunshola, O. C. Daodu, A. Ajadi, S. F. Ambali
    Tropical Animal Health and Production, 2020
  • Fatal multiple intestinal intussusceptions and torsion secondary to a non-degradable foreign body gastrointestinal obstruction in a captive lion (Panthera leo)
    Olatunde Akanbi, Mohammed Adam, Henry Jegede, Abdullateef Ajadi, Jamila Atata, Ibrahim Raufu, Julius Aiyedun, Shodeinde Shoyinka
    Brazilian Journal of Veterinary Pathology, 2020
  • Neglected zoonotic diseases in nigeria: Role of the public health veterinarian
    Nusirat Elelu, Julius Olaniyi Aiyedun, Ibraheem Ghali Mohammed, Oladapo Oyedeji Oludairo, Ismail Ayoade Odetokun, Kaltume Mamman Mohammed, James Olaniyi Bale, Saka Nuru
    Pan African Medical Journal, 2019
  • Awareness and antibody detection of Newcastle disease virus in a neglected society in Nigeria
    Oluwafemi Babatunde Daodu, Julius Olaniyi Aiyedun, Rafiu Adebisi Kadir, Hauwa Motunrayo Ambali, Oladapo Oyedeji Oludairo, Isaac Dayo Olorunshola, Oluwakemi Christiana Daodu, Saka Saheed Baba
    Veterinary World, 2019
  • Assessment of antibody assay methods in determination of prevalence of infectious bursal disease among local chickens and guinea fowls in Kwara state, North Central Nigeria
    Oluwafemi Babatunde Daodu, Oladapo Oyedeji Oludairo, Julius Olaniyi Aiyedun, Hauwa Motunrayo Ambali, Rafiu Adebisi Kadir, Oluwakemi Christiana Daodu, Isaac Dayo Olorunshola, Arimie Deborah Adah
    Veterinary World, 2018
  • Roles of wildlife in epidemiology of rabies: A mini-review
    Julius Aiyedun, Oladapo Oludairo, Isaac Olorunshola
    Journal of Advanced Veterinary and Animal Research, 2017
  • Seroepidemiological survey of bovine brucellosis in selected Fulani Herds in Kwara State, Nigeria
    Julius Aiyedun, Oladapo Oludairo, Isaac Olorunshola, Nathan Furo, Francis Olowoleni, Mohammed Adam, Shodeinde Shoyinka
    Journal of Advanced Veterinary and Animal Research, 2017
  • Prevalence of intestinal parasitism of swine in a North Central State of Nigeria
    Julius Aiyedun, Oladapo Oludairo
    Journal of Advanced Veterinary and Animal Research, 2016
  • An overview of the health and management challenges of rural poultry stock in North Central Nigeria
    Julius Aiyedun, Oladapo Oludairo
    Journal of Advanced Veterinary and Animal Research, 2016
  • Prevalence of antibody against rabies among confined, free-roaming and stray dogs in a transit city of Nigeria
    Veterinaria Italiana, 2011