Response surface methodology optimisation enhancing lactic acid production from Prosopis africana pods by Rhizopus oryzae El-Imam Amina Ahmed, Mardhiyah A. Sanusi, Eromosele Ighalo, Mushafau A. Oke, Patricia F. Omojasola Research Journal of Biotechnology, 2024 Microbial fermentation of lignocellulosic biomass into lactic acid (LA) has received considerable attention because it ensures the valorisation of wastes and reduces dependence on fossil sources. First, the proximate and phytochemical compositions of Prosopis africana pods (PAP) were determined. Previous biologically-pretreated pods of Prosopis africana were then saccharified using dilute acid hydrolysis following a full factorial design. The factors optimized were acid type (HCl and H2SO4), acid concentration (1 %, 3 % and 5 %), solid loading ratio (5 %, 10 % and 20 %) and reaction time (15, 30 and 60 minutes). Several Rhizopus oryzae isolates were screened for LA production and the most prolific was molecularly identified. The factors affecting LA yield from PAP hydrolysate were screened using a half-factorial design. The significant factors were then optimised using Box-Behnken Design of Response Surface Methodology (RSM). The proximate analysis showed high levels of protein, lipid, ash and carbohydrates. The phytochemical analysis of PAP revealed the presence of flavonoids, alkaloids, saponins, phenols, tannins, glycosides and terpenoids. The hydrolysis conditions of 3 % HCl, 20 % solid loading and 15 minutes hydrolysis time yielded the highest reducing sugars of 42.5 g/L. The most promising isolate, identified as R. oryzae strain AK-22, produced 19.7 g/L after RSM optimization, a 38.1 % increase over yields from non-optimised conditions. These findings are on the biotechnological production of LA from the pods of Prosopis africana, an abundant yet under-utilised tree crop.
Submerged fermentation of orange albedo to produce gibberellic acid using Fusarium moniliforme and Aspergillus niger Jordan Journal of Biological Sciences, 2018
Bioconversion of chicken feather waste: optimizing keratinase production by Pseudomonas aeruginosa PA1045 and Alcaligenes faecalis IHB B 6507, characterization and application … FO Otuyelu, PF Omojasola, OO Adebisi, J Maiangwa, RT Azeez, AA Ali, ... International Microbiology 28 (8), 3147-3160 , 2025 2025 Citations: 1
Screening and Identification of Keratin-degrading Microorganisms Isolated from Different Chicken-feather Waste Dumpsite Soils in Ilorin Metropolis, Nigeria FO Otuyelu, PF Omojasola, OO Adebisi, RT Azeez, ZB Abdulsalam, ... Journal of Applied Sciences and Environmental Management 29 (5), 1472-1478 , 2025 2025 Citations: 1
SCREENING AND GROWTH OPTIMIZATION OF BIOSURFACTANT-PRODUCING FUNGI IN AGRO-WASTE (SORGHUM BRAN) MEDIA FOR BIOREMEDIATION STRATEGIES RB Tomilayo, PF Omojasola, OO Frank, DO Adetitun, RT Azeez FUDMA JOURNAL OF SCIENCES 9 (4), 223-228 , 2025 2025
Characterization of Silver Nanoparticles Biosynthesized using Keratinase from Aspergillus species and their Antibacterial Activity against Clinical Pathogens FO Otuyelu, OO Adebisi, PF Omojasola, RT Azeez, ZB Abdulsalam, ... BioNanoScience 15 (1), 136 , 2025 2025 Citations: 4
Response surface methodology optimization enhancing lactic acid production from Prosopis africana pods using Rhizopus oryzae PF Ahmed El Imam, A., Ighalo, E., Sanusi, M., Oke, M.A. and Omojasola Research Journal of Biotechnology 19 (1), 91-104 , 2024 2024
Isolation, identification and screening of humic acid producing fungi from soil environment of oil palm (Elaeis guineensis) associated with empty fruit bunches SI Kawata, H.M., Omojasola, P.F., Ajiboye, A.E., Adedayo, M.R. and Bale UMYU Journal of Microbiology Research 8 (2), 165-173 , 2023 2023 Citations: 8
Enzyme and Biosurfactant Production by Exopolysaccharide Producing Bacteria Isolated from Cassava Peel Heaps. AG Amao, J.A., Omojasola, P.F., Ayandele, A.A. and Adewoyin Moscow University Biological Sciences Bulletin 78 (4), 232-240 , 2023 2023 Citations: 2
Bioethanol Production from Biologically Pretreated Prosopis africana Pods using Pichia kudriavzevii SY4. AA El-Imam, E Ighalo, M Sanusi, MA Oke, PF Omojasola Jordan Journal of Biological Sciences 15 (5) , 2022 2022 Citations: 2
Effects of evaporative cooling storage on microbiological and nutritional quality of Telfairia occidentalis Hook. f. and Amaranthus hybridus L. PF Omojasola, IG Adarabierin, MA Oke Ife Journal of Science 24 (2), 237-250 , 2022 2022 Citations: 2
Extension of the shelf life of tomato fruit (Solanum lycopersicum) using wood ash PF Omojasola, RA Abdulsalam, MA Oke Thai Journal of Agricultural Science 55 (3), 161− 174-161− 174 , 2022 2022 Citations: 7
Utilization of Pachysolen tannophilus and Pichia kudriavzevii for the production of xylitol on undetoxified corn cob hydrolysates. PF Jolayemi, O., Oke, M.A., Rocha-Meneses, L. and Omojasola Agronomy Research 20 (1), 188-199, https://doi.org/10.15159/AR.22. , 2022 2022 Citations: 4
Phosphate solubilization potential of indigenous rhizosphere fungi and their biofertilizer formulations. PF Balogun, D.A., Oke, M.A., Rocha-Menenses, L., Fawole, O.B. and Omojasola Agronomy Research 20 (https://doi.org/10.15159/AR.21.163), 40-55 , 2022 2022 Citations: 23
Production of Collagenase by Aspergillus niger and Aspergillus terreus Isolates using Scales of Tilapia and Croaker Fishes MO Omojasola, P.F., Adeniran, F., Saliu, B.K. and Kazeem Cameroon Journal of Biological and Biochemical Sciences 28 (2), 131-145 , 2020 2020 Citations: 1
Comparative 16S rDNA metagenomics study of two samples of cassava peel heap from Nigeria and India JA Amao, M Barooah, PF Omojasola 3 Biotech 9 (11), 418 , 2019 2019 Citations: 4
Isolation and characterization of some exopolysaccharide producing bacteria from cassava peel heaps JA AMAO, PF Omojasola, M Barooah Scientific African 4, e00093 , 2019 2019 Citations: 28
Physiological and bacteriological profile of the fish Clarias gariepinus (Siluriformes: Clariidae) chronically exposed to different concentrations of municipal waste leachate … OD Owolabi, PF Omojasola, FJ Abioye, OP Aina Cuadernos de Investigación UNED 11 (2), 182-197 , 2019 2019 Citations: 4
Submerged Fermentation of Jatropha curcas Seedcake in Production of Itaconic Acid by Aspergillus niger and Aspergillus terreus P OKWECHIME, P OMOJASOLA Egyptian Society of Biological Sciences , 2019 2019
Laboratory Practicals in Microbiology II AB Olayemi, A Sani, GP Oyeyiola, PF Omojasola, MO Kolawole, ... The Department of Microbiology, University of Ilorin, Ilorin , 2018 2018
Gibberellic acid production by Fusarium moniliforme and Aspergillus niger using submerged fermentation of banana peel PF Omojasola, DO ADEJORO Notulae Scientia Biologicae 10 (1), 60-67 , 2018 2018 Citations: 13
Toxicological studies on Terminalia glaucescens using the animal model. PF Okutue, J.U. and Omojasola Centrepoint (Science Edition) 24 (2), 224-236 , 2018 2018
MOST CITED SCHOLAR PUBLICATIONS
Cellulase production by some fungi cultured on pineapple waste PF Omojasola, OP Jilani, SA Ibiyemi Nature and science 6 (2), 64-79 , 2008 2008 Citations: 166
The antibacterial activity of the leaf extracts of Anacardium occidentale and Gossypium hirsutum against some selected microorganisms PF Omojasola, S Awe Biosci Res Commun 16 (1), 25-28 , 2004 2004 Citations: 83
Cellulase production by Trichoderma longi, Aspergillus niger and Saccharomyces cerevisae cultured on waste materials from orange PF Omojasola, OP Jilani Pakistan Journal of Biological Sciences 11 (20), 2382-2388 , 2008 2008 Citations: 72
The effect of different concentrations of ginger on the quality of smoked dried catfish (Clarias gariepinus) GL Idris, FS Omojowo, PF Omojasola, CO Adetunji, EO Ngwu Nature and Science 8 (4), 59-63 , 2010 2010 Citations: 57
Cellulase production by Trichoderma longi , Aspergillus niger and Saccharomyces cerevisae cultured on plantain peel. PF Omojasola, OP Jilani 2009 Citations: 46
Microbiological quality of fresh catfish raised in ponds fertilized with raw sterilized poultry manures FS Omojowo, PF Omojasola FISON , 2013 2013 Citations: 29
Isolation and characterization of some exopolysaccharide producing bacteria from cassava peel heaps JA AMAO, PF Omojasola, M Barooah Scientific African 4, e00093 , 2019 2019 Citations: 28
Phosphate solubilization potential of indigenous rhizosphere fungi and their biofertilizer formulations. PF Balogun, D.A., Oke, M.A., Rocha-Menenses, L., Fawole, O.B. and Omojasola Agronomy Research 20 (https://doi.org/10.15159/AR.21.163), 40-55 , 2022 2022 Citations: 23
A comparative study of the antibacterial activity of Piliostigma reticulatum bark extract with some antibiotics S Awe, PF Omojasola Ethnobotanical leaflets 2009 (9), 11 , 2009 2009 Citations: 22
Antibiotic resistance pattern of bacterial pathogens isolated from poultry manure used to fertilize fish ponds in new Bussa, Nigeria F Omojowo, F Omojasola Albanian Journal of Agricultural Sciences 12 (1), 81 , 2013 2013 Citations: 21
Biomolecular characterization, identification, enzyme activities of molds and physiological changes in sweet potatoes ( Ipomea batatas ) stored under controlled … CO Oladoye, IF Connerton, RMO Kayode, PF Omojasola, IB Kayode Journal of Zhejiang University-SCIENCE B 17 (4), 317-332 , 2016 2016 Citations: 19
Evaluation of citric acid and potassium sorbate as preservatives on the safety and shelf-life of smoked catfish FS Omojowo, PF Omojasola, GL Idris, JA Ihuahi Nat. Sci 7 (11), 1-8 , 2009 2009 Citations: 19
Phytochemical screening of some selected tropical African Mosses PO Fatoba, PF Omojasola, S Awe, FG Ahmed Nigerian Society for Experimental Biology (NISEB) Journal 3 (2), 49-52 , 2003 2003 Citations: 18
Antibiotic resistance pattern of bacterial pathogens isolated from cow dung used to fertilize Nigerian fish ponds FS OMOJOWO, FP OMOJASOLA Notulae Scientia Biologicae 5 (1), 15-19 , 2013 2013 Citations: 17
Effect of brining on the microbial quality and safety of smoked catfish FS Omojowo, PF Omojasola, MO Kolawole, EO Ngwu, GB Oluborode, ... New York Science Journal 3 (6), 20-26 , 2010 2010 Citations: 16
The production of itaconic acid from sweet potato peel using Aspergillus niger and Aspergillus terreus PF Omojasola, EA Adeniran Albanian Journal of Agricultural Sciences 13 (4), 72-77 , 2014 2014 Citations: 14
Gibberellic acid production by Fusarium moniliforme and Aspergillus niger using submerged fermentation of banana peel PF Omojasola, DO ADEJORO Notulae Scientia Biologicae 10 (1), 60-67 , 2018 2018 Citations: 13
Synthesis, Characterization, Crystal Structure and Antimicrobial Evaluation of a Novel --M-X-M-X-- Type Infinite Chain 1D Cu(II) Complex with Eflornithine Hydrochloride … OPF Obaleye JA, Tella AC, Osunniran WA, Simon N Journal of Inorganic and Organometallic Polymers and Materials 24 (5), 827-835 , 2014 2014 Citations: 13
Antibacterial Effect of Honey on Bacterial Isolated From Infected Wounds in Ilorin, Nigeria PF Omojasola NISEB Journal 2 (2), 109-112 , 2002 2002 Citations: 11
Microbial quality of citric acid as preservatives in smoked catfish (Clarias gariepinus) FS Omojowo, PF Omojasola, JA Ihuahi J. Bio. and Env. Sci 5 (3), 130-134 , 2008 2008 Citations: 10