Md Amirul Alam
@ums.edu.my
Senior Lecturer, Faculty of Sustainable Agriculture (FPL) Universiti Malaysia Sabah (UMS), Sandakan Campus
Universiti Malaysia Sabah
Dr. Md Amirul Alam
Senior Lecturer
Faculty of Sustainable Agriculture (FPL)
Universiti Malaysia Sabah (UMS), Sandakan Campus
Locked bag no. 3, 90509 Sandakan, Sabah
Mobile: +6 0102172491
Email: amirulalam@
EDUCATION
2015: PhD in Agronomy, Universti Putra Malaysia, Serdang, Selangor, Malaysia
2008: Master of Science (M.S.) in Genetics & Plant Breeding, Bangladesh Agricultural University, Bangladesh.
2003: (Held in 2006) B. Sc. in Agriculture, (Honours), Patuakhali Science and Technology University, Dumki, Patuakhali, Bangladesh.
1998: Higher Secondary School Certificate (HSC), Cantonment College, Jessore, Bangladesh.
1996: Secondary School Certificate (SSC), Baruna Bazar P.D.C. High School, Khulna, Bangladesh.
RESEARCH, TEACHING, or OTHER INTERESTS
Agricultural and Biological Sciences, Agronomy and Crop Science, Horticulture, Plant Science
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
Moomtahina Rahman, Md Alauddin, G. M. Mohsin, Md. Amirul Alam, and M. K. Rahman
Update Publishing House
Rooftop vegetable productions are becoming an important part of the recent rejuvenation of urban agriculture particularly in densely populated urban areas. However, due to weight limits often associated with rooftop growing systems, rooftop plant growing media including soil, may not contain optimal fertility levels required to maximize plant growth and productivity. Therefore, the success of rooftop vegetable production often depends on proper fertility management schemes to create optimal plant growing conditions. Therefore, an experiment was conducted to investigate combine impacts of composted poultry manure (CPM) and inorganic fertilizers on growth and yield of tomato under rooftop growing conditions. The experiment was arranged in a completely randomized design (CRD) with eight treatments and three replications. The treatments included T1: Control (no CPM and no NPK), T2: 4 ton CPM ha-1, T3: N55P15K50 kg ha-1, T4: N110P30K100 kg ha-1, T5: N165P45K150 kg ha-1, T6: 4 ton CPM ha-1+ N55P15K50 kg ha-1, T7: 4 ton CPM ha-1+ N110P30K100 kg ha-1, and T8: 4 ton CPM ha-1+N165P45K150 kg ha-1. The results showed that NPK fertilizers alone and CPM combined with NPK fertilizers greatly improved tomato plant growth and fruit yield. However, maximum plant growth and tomato yields (68 t ha-1) and economic benefits (benefit: cost ratio 6.9) were achieved with 4 t ha-1 CPM amendment and 100% recommended doses of NPK fertilizers. Control treatment (T1: -CPM, -NPK) had the lowest tomato yield (6.2 t ha-1). Composted poultry manure alone contributed for around 15.0 t ha-1 tomato yield and supplemented for around 30 kg ha-1 N fertilizer. Combine application of 4 t ha-1 CPM and 100% RDF of NPK fertilizers indicated as the agreeable combination in this study for optimal tomato plant growth and yield under rooftop growing conditions.
Yawo Mawunyo Nevame Adedze, Xia Lu, Wenyi Fan, Wenting Zhang, Xue Yang, Zhijun Deng, Md. Amirul Alam, Guangli Xu, Lihua Zhang, and Wenhu Li
Czech Academy of Agricultural Sciences
B. C. Kundu, G. M. Mohsin, M. S. Rahman, F. Ahamed, A. K. Mahato, K. M. Delowar Hossain, M. B. Jalloh, and Md. Amirul Alam
FapUNIFESP (SciELO)
Abstract Combining ability analysis provides useful information for the selection of parents, also information regarding the nature and magnitude of involved gene actions. Crops improvement involves strategies for enhancing yield potentiality and quality components. Targeting the improvement of respective characters in bitter gourd, combining ability and genetic parameters for 19 characters were estimated from a 6×6 full diallel analysis technique. The results revealed that the variances due to general combining ability (GCA) and specific combining ability (SCA) were highly significant for most of the important characters. It indicated the importance of both additive and non-additive gene actions. GCA variances were higher in magnitude than SCA variances for all the characters studied indicating the predominance of the additive gene effects in their inheritance. The parent P2 (BG 009) appeared as the best general combiner for earliness; P1 (BG 006) for number of fruits, average single fruit weight and fruit yield; P4 (BG 027) for node number of first female flower and days to seed fruit maturity; P3 (BG 011) for fruit length and thickness of the fruit flesh; P5 (BG 033) for 100-seed weight; and P6 for number of nodes per main vine. The SCA effect as well as reciprocal effect was also significant for most of the important characters in different crosses.
M. S. H. Bhuiyan, M. A. Malek, R. M. Emon, M. K. Khatun, Mohammad Moneruzzaman Khandaker, and Md. Amirul Alam
FapUNIFESP (SciELO)
In soybean breeding program, continuous selection pressure on traits response to yield created a genetic bottleneck for improvements of soybean through hybridization breeding technique. Therefore an initiative was taken to developed high yielding soybean variety applying mutation breeding techniques at Plant Breeding Division, Bangladesh Institute of Nuclear Agriculture (BINA), Bangladesh. Locally available popular cultivar BARI Soybean-5 was used as a parent material and subjected to five different doses of Gamma ray using Co60. In respect to seed yield and yield attributing characters, twelve true breed mutants were selected from M4 generation. High values of heritability and genetic advance with high genotypic coefficient of variance (GCV) for plant height, branch number and pod number were considered as favorable attributes for soybean improvement that ensure expected yield. The mutant SBM-18 obtained from 250Gy provided stable yield performance at diversified environments. It provided maximum seed yield of 3056 kg ha-1 with highest number of pods plant-1 (56). The National Seed Board of Bangladesh (NSB) eventually approved SBM-18 and registered it as a new soybean variety named 'Binasoybean-5' for large-scale planting because of its superior stability in various agro-ecological zones and consistent yield performance.
Md. Amirul Alam, Abdul Shukor Juraimi, M. Y. Rafii, Azizah Abdul Hamid, Farzad Aslani, and M. A. Hakim
Springer Science and Business Media LLC
Md. Amirul Alam, M.S.H. Bhuiyan, M.A. Malek, R.M. Emon, Khadija Khatun, and Humayun Kobir
Agricultural Research Communication Center
Background: Soybean is an excellent source of protein, also richer in oil than most legumes, making them a good source for vegetable oil and biofuels. Among various difficulties the maturity period of existing soybean varieties is the main hindrance of utilizing this for the existing cropping system. The narrow genetic base of cultivated soybean varieties and germplasm limit the scope to utilize directly in the breeding program. Methods: Mutation breeding is one of the techniques that provide large genetic diversity from a single source. To broaden the genetic diversity Binasoybean-3 and Binasoybean-4 were imposed to different doses of gamma radiation. The mutants were selected based on their agronomic performance and grouped at five different clusters at M5 generations. Maximum selection pressure was done during maturity period with protein and oil content. Result: Finally, eight mutants were selected for the advance breeding program, whereas mutants SM-03-15-5 mature within 90 days, containing 38% protein and 18.4% oil content will be considered directly for further steps of varietal release system. Background: Soybean is an excellent source of protein, also richer in oil than most legumes, making them a good source for vegetable oil and biofuels. Among various difficulties the maturity period of existing soybean varieties is the main hindrance of utilizing this for the existing cropping system. The narrow genetic base of cultivated soybean varieties and germplasm limit the scope to utilize directly in the breeding program.
Reza M. Emon, Md. N. Sakib, Mst. K. Khatun, Md. A. Malek, Md. S. Haque, and Md. Amirul Alam
Update Publishing House
Identification of genetic diversity and their relationships among breeding materials is crucial in crop improvement strategies. In this study, 38 sesame genotypes were characterized for their genetic diversity. The results revealed significant variations among various traits such as plant height, maturity, capsule plant-1 and seeds capsule-1. The number of capsule plant-1 showed significant positive correlation with seeds capsule-1. The highest heritability was found for the numbers of capsules plant-1 (98.67%). The 38 genotypes were separated into six distinct clusters. Comparison within the populations of the cluster IV and those of cluster VI had the highest capsules plant-1, seeds capsule-1 with enormous genetic diversity. For molecular characterization, 7 microsatellite markers and 5 SSR primers with polymorphism were finally chosen for genetic diversity analysis. Altogether, 19 alleles were identified among the 38 genotypes, and the average number of alleles per locus was 3.80. The lowest and the highest numbers of alleles were 3 and 5, respectively. The polymorphism information content (PIC) ranged from 0.3201 to 0.5934 and SI-ssr30 showed to be highest at 0.5934. The UPGMA based clustering depicted a significant variation at molecular level among the sesame genotypes, having a coefficient of similarity between 0.29 and 1.00. The present study confirmed that extensive genetic diversity existed among the sesame genotypes and valuable agronomic traits may result in the development of high yielding genotypes.
Md. Amirul Alam, Md. Alauddin, Moomtahina Rahman, Md. Alauddin, Md. Sajedur Rahman, G. M. Mohsin, and Md. Khalilur Rahman
Update Publishing House
Rooftop farming is gaining rapid popularity in urban areas, especially since the beginning of the global COVID-19 pandemic. For housebound people rooftop farming is not only a way of potentially management of their time but also the execution of creativity. For rooftop farming vermicompost (VC) can be the most sustainable media for growing high value fruits and vegetables crops. In this regard, an experiment was carried out as a rooftop farming system at Charfassion upzila in the Bhola district of Bangladesh to observe the growth and yield performance of capsicum (Capsicum annuum) grown in different combinations of vermicompost in the winter season (2020-2021). Different agronomic and yield parameters were measured at the intervals of 30, 60, and 90 days and at the final harvest of 120 days after sowing. Among the measured parameters a mixed type of performance was achieved from varied doses of vermicompost applications and inorganic fertilizer treatment. Among the measured valuable parameters; the highest fruit length (8.85 cm), fruit diameter (10.3 cm), no. of fruits (9.51 plant-1), total wt. of fresh fruits (405.32 g/plant), fresh fruits yield (11.26 t ha-1), no. of branches (30.0 plant-1), stem girth (6.3 cm), root fresh wt. (8.80 g/plant), stem fresh wt. (23.35 g/plant), petiole fresh wt. (6.09 g/plant), total fresh biomass (65.54 g/plant), dry wt. of fruits (76.91 g/plant), dry wt. of total fruits: biomass ratio (7.35) and benefit-cost ratio (12.40), respectively, were observed in T5 (20t VC ha-1). So, from the overall findings of this study T5 with 20t VC application ha-1 is recommended to achieve better growth and yield of capsicum through the sustainable way of rooftop and urban farming systems and to improve soil fertility status.
Ivy Quirinus Chong, Elisa Azura Azman, Ji Feng Ng, Roslan Ismail, Azwan Awang, Nur Aainaa Hasbullah, Rosmah Murdad, Osumanu Haruna Ahmed, Adiza Alhassan Musah, Md. Amirul Alam,et al.
MDPI AG
In Malaysia, the main constraints of rice yield and productivity are infertile soils and poor management practices because these soils are characterized by low pH, low nutrient availability, low organic matter, and high exchangeable Al and Fe ions, due to high rainfall and hot temperatures. Thus, an incubation study was conducted to determine the optimum amount of calcium silicate (HmbG brand) to improve the soil pH, electrical conductivity (EC), exchangeable Al, available P, and cation exchange capacity (CEC) of a paddy soil in Sabah, Malaysia. The Kelawat series (Typic Dystrudept) soil was incubated with calcium silicate at the application rates of 0 (T1), 1 (T2), 2 (T3), and 3 t ha−1 (T4) using a Completely Randomized Design (CRD) in triplicates for 30, 60, 90, and 120 days. The calcium silicate used significantly improved soil pH because of the release of SiO44− and Ca2+ ions, which neutralized and immobilized H+ ions. Furthermore, the neutralizing effects of the amendment impeded Al hydrolysis by up to 57.4% and this resulted in an increase in the available P in the soil by 31.26% to 50.64%. The increased availability of P in the soil was also due to the high affinity of SiO44− to desorb P from soil minerals and it is believed that SiO44− can temporarily adsorb exchangeable base cations such as K+, Ca2+, Mg2+, and Na+. Moreover, applying calcium silicate at 3 t ha−1 improved soil CEC by up to 54.84% compared to that of untreated soils (T1) because of increased pH and the number of negatively charged sites. The most suitable application rate of the calcium silicate was found to be 3 t ha−1 (T4). These findings suggest that calcium silicate can improve soil productivity and agronomic efficiency in rice farming. Greenhouse and field trials are necessary to ascertain the effects of the recommended treatments of this incubation study on soil productivity, rice growth, and yield.
M.A. Malek, R.M. Emon, M.K. Khatun, M.S.H. Bhuiyan, Adedze Yawo Mawunyo Nevame, and Md. Amirul Alam
Agricultural Research Communication Center
Background: Soybean is an important source of food, protein and oil and hence more research is essential to increase its yield under different agro-ecological conditions, including stress. In this regard, four popular soybean varieties viz. Shohag, BDS-4, BAU-S/64 and BARI Soybean-5 were irradiated using Co60 gamma rays to create genetic variation for earliness, higher seed yield and other desirable agronomic traits. Methods: The experiments were conducted at Bangladesh Institute of Nuclear Agriculture (BINA) Headquarters farm, Mymensingh during 2006-2009 and 28 elite mutant lines were selected for evaluation. The mutant line, SBM-22 derived from mother variety BARI Soybean-5 irradiated with 300Gy of gamma rays was found to be superior compared to other mutants. Considering the superior performance of mutant SBM-22 including 28 mutants and mother check variety BARI Soybean-5, were evaluated through different trials. The evaluation trials were conducted at different agro-ecological zones of the country during Rabi season (January to April) of 2010-2018. Result: Significant variations were observed both in individual location and over locations for all traits. Reactions to major diseases and insect-pests infestation were also studied. Due to better performance of the mutant SBM-22, Bangladesh Institute of Nuclear Agriculture (BINA) applied to the National Seed Board (NSB) of Bangladesh for registration as an important soybean variety “Binasoybean-6”. Consequently, the NSB of Bangladesh registered SBM-22 as an improved soybean variety in 2019 as Binasoybean-6 for commercial cultivation.
Md. Amirul Alam, Suhara B. Alias, Januarius Gobilik, Salumiah B. Mijin, and Mohammad M. Khandaker
Basrah Journal of Agricultural Sciences, College of Agriculture, University of Basrah
Moringa oleifera seed solution was used in this study to treat municipal wastewater that were used as the treatment in this study. There were 3 treatments used; treated wastewater, normal tape water and untreated wastewater. The wastewater were collected at main drainage at Batu 7 (5o52’57.2’’N 118o02’39.7”E) and diagnosed based on the pH and EC. Data on plant height (cm), number of leaves, leaves length (cm), chlorophyll, and number of primary branches were taken every week until week 4. For root length (cm), fresh weight (g), dry weight (g) and moisture were taken after the harvesting. The data collected were analyzed by using Statistical Analysis Software (SAS) version 9.4 computer program with experimental design was Randomized Complete Block Design (RCBD). The means were separated and compared using Duncan’s Multiple Range Test (DMRT) at 0.05 significant level. M. oleifera seeds solution treated irrigation exhibited positive outcomes for most of the parameters recorded, but response of different vegetables were also different on varied parameters. The increase of pH from untreated waste water (6.40) to sanitized/treated waste water (6.73) and reduction of EC from untreated waste water (367.9) to sanitized/treated waste water (359.1) is the proof of making nutrients more available for plants uptake. From the overall study it is proved that M. oleifera seeds are suitable as the replacement and an alternative besides chemical coagulant to treat wastewater which is cheaper, eco-friendly and sustainable to be used in agricultural irrigation based on all the parameters evaluated in this study.
M. M. Khandaker, A. Saidi, N. A. Badaluddin, N. Yusoff, A. Majrashi, M. M. Alenazi, M. Saifuddin, Md. A. Alam, and K. S. Mohd
FapUNIFESP (SciELO)
Abstract The wax apple or jambu madu, is a non-climacteric tropical fruit from Myrtaceae family and widely cultivated in South East Asia. The limited availability of good quality seedlings of wax apple is the main problem to development of flourish it’s market share in the current fruit industry. Therefore, in order to produce good quality planting materials, a study aimed at optimizing propagation and adventitious rooting technique and survivability of wax apple air layer was conducted. In this study, four different levels of Indole-3-Butyric Acid (IBA) concentration (0, 1000, 1500 and 2000 mg L-1) and three rooting media (sphagnum moss, vermicompost and garden soil) were applied after removal of bark (phloem) on the shoot to determine the effect on rooting and survivability of the wax apple air layer under field conditions. The results showed that the wax apple shoots treated with 2000 mg L-1 IBA produced the significantly higher number of roots, increased length of root, diameter of branch, length of branch, number of leaf and leaf area of air layers. In addition, the highest chlorophyll content and stomatal aperture were recorded in 2000 mg L-1 IBA treatment compared to other treatments including control. Vermicompost medium was better than garden soil and sphagnum moss in respect of rooting and survivability of air layers. The results showed that the combination of 2000 mg L-1 IBA and vermicompost as rooting media give the best combination to root initiation, root number, root length and survival rate (100%) of wax apple air layers. From this study, it can be concluded that 2000 mg L-1 IBA and vermicompost treatment enhance the root initiation, early establishment and survivability of wax apple air layered under field conditions.
Yawo Mawunyo Nevame Adedze, Xia Lu, Yingchun Xia, Qiuyue Sun, Chofong G. Nchongboh, Md. Amirul Alam, Menghua Liu, Xue Yang, Wenting Zhang, Zhijun Deng,et al.
Springer Science and Business Media LLC
AbstractInsertion and Deletion (InDel) are common features in genomes and are associated with genetic variation. The whole-genome re-sequencing data from two parents (X1 and X2) of the elite cucumber (Cucumis sativus) hybrid variety Lvmei No.1 was used for genome-wide InDel polymorphisms analysis. Obtained sequence reads were mapped to the genome reference sequence of Chinese fresh market type inbred line ‘9930’ and gaps conforming to InDel were pinpointed. Further, the level of cross-parents polymorphism among five pairs of cucumber breeding parents and their corresponding hybrid varieties were used for evaluating hybrid seeds purity test efficiency of InDel markers. A panel of 48 cucumber breeding lines was utilized for PCR amplification versatility and phylogenetic analysis of these markers. In total, 10,470 candidate InDel markers were identified for X1 and X2. Among these, 385 markers with more than 30 nucleotide difference were arbitrary chosen. These markers were selected for experimental resolvability through electrophoresis on an Agarose gel. Two hundred and eleven (211) accounting for 54.81% of markers could be validated as single and clear polymorphic pattern while 174 (45.19%) showed unclear or monomorphic genetic bands between X1 and X2. Cross-parents polymorphism evaluation recorded 68 (32.23%) of these markers, which were designated as cross-parents transferable (CPT) InDel markers. Interestingly, the marker InDel114 presented experimental transferability between cucumber and melon. A panel of 48 cucumber breeding lines including parents of Lvmei No. 1 subjected to PCR amplification versatility using CPT InDel markers successfully clustered them into fruit and common cucumber varieties based on phylogenetic analysis. It is worth noting that 16 of these markers were predominately associated to enzymatic activities in cucumber. These agarose-based InDel markers could constitute a valuable resource for hybrid seeds purity testing, germplasm classification and marker-assisted breeding in cucumber.
Umar Aliyu Abdullahi, Mohammad Moneruzzaman Khandaker, Nadiawati Alias, Elyni Mat Shaari, Md. Amirul Alam, Noor Afiza Badaluddin, and Khamsah Suryati Mohd
Update Publishing House
Seaweeds are plants found in sea that have tremendous applications in the fields of agriculture and environment. It comprises of three giant classes with a large number of different species. their ability to adopt to various conditions qualifies them more applicable to various environmental and agricultural arena. Agriculturally, both three classes Phaeophyta, Rhodophyta and Chlorophyta, have significant roles in promoting plant growth and productivity and soil protection as well as reclamation with class Phaeophyta has highest contribution due to its alginic acid content and other multifaceted components that are higher followed by Rhodophyta and Chlorophyta. Seaweed (living or dead biomass) has ability to phycoremediate environment against heavy toxic metals and lessen the excessiveness of non-metal inorganic elements via physisorption, chemisorption with the aid of binding sites provided by proteins and carbohydrates functional groups existing in their cell walls and secretion of organic acids and intracellular transformation and accumulation. Seaweed is an important factor in environmental remediation and soil restoration processes.
Mohammad M. Khandaker, Nurul H. Ismail, Umar A. Abdullahi, Md. A. Alam, Noor A. Badaluddin, and Khamsah S. Mohd
Basrah Journal of Agricultural Sciences, College of Agriculture, University of Basrah
This study was conducted to evaluate the potential effects of branch bending angle on the plant physiological characteristics, mineral nutrients accumulation and fruit quality of wax apple. Different treatments with five replicates each by Randomized Complete Block Design (RCBD) layouts were arranged in this study. All the treatments represent different branch angles which were at 5˚ (control), 20˚, 45˚, 65˚ and 85˚ from vertical axis. Leaf chlorophyll content (SPAD chlorophyll index) was significantly affected by different bending treatments at a budding stage, flowering, fruit development and after harvesting of wax apple. Total soluble solids (TSS) content in the leaves of bent branches increased significantly at before bud development, flowering, fruit ripening and after harvesting stages of wax apple trees. During fruit development and maturation stages, leaf TSS content was decreased in all bent branches. The results showed that 20˚ to 65˚ angle bent branches increase weight and firmness of fruit, fruit diameter and produced dark-coloured fruit compared to control. The results showed that 65˚ bent branches give the highest potassium (K+) and magnesium (Mg2+) content in the fruits. Higher sodium (Na+), iron (Fe2+) and calcium (Ca2+) content in fruits were found in 20˚ bent branches. There was a positive correlation between fruit TSS with Mg2+ (r = 0.70), with Na+ (r = 0.67) and with Ca2+ (0.57) content in the bent branches of wax apple. Fruit TSS content also positively correlated with firmness and peel colour of wax apple fruits. The number of fruit, fruit weight and fruit diameter of wax apple also positively correlated (weak) with leaves TSS content. It can be concluded that 20˚ to 65˚ branch bending angle are promising for enhancing plant physiology, fruit growth and quality of wax apple fruits.
Wan Nur Suzani Sazleen Wan Shafiin, Nurfatin Liyana Ablah, Hasan Nudin Nur Fatihah, Md Amirul Alam, Rohayu Ma’arup, Md Sarwar Jahan, Kamarul Ain Mustafa, and Nadiawati Alias
Informa UK Limited
ABSTRACT Micronutrient malnutrition is a major problem in developing countries affecting women, children and infants. Biofortification, improved soil health and waste management efficiencies have been used to overcome nutrient deficiencies. Application of classical breeding and selection resulted in rapid development of staple crops through breeding. The Cucurbitaceae mainly pumpkins and squashes (Cucurbita moschata Duch., Cucurbita pepo L., Cucurbita maxima Duch. ex Lam), cucumber (Cucumis sativus L.), watermelon (Citrullus lanatus L.), and melon (Cucumis melo L.) possess beneficial vitamins and minerals that can be used as nutrients for human consumption. To improve nutritional content in Cucurbitaceae, understanding the information in the genome is important for breeders to increase the level of nutrition and quality of characteristics using biotechnology approaches. Nonconventional breeding approaches can assist conventional breeding to save time, costs, and efficacy in selection. This review describes breeding strategies for necessary solutions toward development of elite varieties of Cucurbitaceae via various approaches that contain high nutrition content and improve traits and quality to fight micronutrient malnutrition.
Md. Amirul Alam, T. A. Nadirah, Gazi Md Mohsin, M. Saleh, K. M. Moneruzzaman, Farzad Aslani, Abdul Shukor Juraimi, and M. Z. Alam
Informa UK Limited
ABSTRACT Natural sources may be a method to introduce antioxidants in the human diet. Portulaca oleracea L., Peperomia pellucida L., and Trianthema portulacastrum L are underutilized leafy vegetables. The plants may be sources for total phenolic (TPC), total flavonoid (TFC), total carotenoid (TCC), and β-carotene but information is lacking. Antioxidant activity, ferric reducing antioxidant power (FRAP), and 1,1-diphenyl-2-picrylhydrazyl (DPPH), and macro- and microminerals contents were investigated in P. oleracea, P. pellucida and T. portulacastrum. Trianthema portulacastrum had the highest TPC (1.34 ± 0.13 mg∙g−1), TFC (0.58 ± 0.08 mg∙g−1), TCC (0.14 ± 0.01 mg∙g−1), and β-carotene (0.94 ± 0.18 mg∙g−1) values. The antioxidant activity and FRAP assay were highest in P. oleracea (0.91 ± 0.09 mg∙g−1); the DPPH assay for P. pellucida was highest (IC50 = 7 µg∙mL−1). The amount of K was highest in P. oleracea (55.470 ± 0.481 g∙kg−1). The amount of Mn was highest in P. pellucida (0.251 ± 0.002 g∙kg−1). The individual underutilized vegetables may be able to supply some antioxidant compounds and mineral contents for the human diet.
Md. Amirul Alam, M. Saleh, Gazi Md. Mohsin, T. A. Nadirah, Farzad Aslani, M. M. Rahman, Siddhartha Kumar Roy, Abdul Shukor Juraimi, and M. Z. Alam
Hindawi Limited
A.Y.M. Nevame, Lu Xia, Zhang Wenting, Chofong G. Nchongboh, Li Wenhu, Muhammad M. Hasan, Md. Amirul Alam, and Si Longting
Science Society of Thailand
Marker-assisted selection (MAS) is a tool that is widely applied in tomato resistance breeding. To determine the robustness of some molecular markers commonly used in MAS, extensive screening of 964 tomato lines was performed under a controlled experimental condition. Initially, the application of 36 molecular markers targeting 26 resistance genes (R genes) and 14 major diseases was evaluated. Here, we employed basic molecular biology and bioinformatics techniques for analysis where polymorphism, accuracy and clearness of amplicons constituted the selection criteria of markers. Upon initial analysis, 20 of these markers designated as efficient markers, among which 8 were considered gene-based markers and referred to as perfect markers were selected for detail evaluation. Information extrapolated from PCR result revealed 18 R genes that control 12 diseases were grouped under efficient markers. On the other hand, grouping of breeding lines based on the number of R gene harbored comprehensively revealed 62% of the lines to be void of R gene, while 38% carry different types of R genes. This provides us with an avenue to better understand new sources of resistance in the breeding lines. Conclusively, these efficient markers and their limited PCR condition can be suggested as basis of a diagnostic kit for MAS applications against 12 major tomato diseases and the identified resistant breeding lines could be conserved in order to be propagated as different sources of resistance for the development of new resistant varieties. Therefore, in areas with high vulnerability to diseases, high efficiency combination of the relevant R genes and their pyramiding into commercial tomato varieties are proposed to be implemented as a pragmatic approach.
F. Yasmine, M. A. Rahman, M. M. Hasan, Md. Amirul Alam, M. S. Haque, M. R. Ismail, and M. Y. Rafii
Agricultural Research Communication Center
The study sought to evaluate the response of three groundnut genotypes (Binachinabadam-3, Dacca-1 and Zhingabadam) to various salt concentrations (D0 – Control, D1 – 2.5 dS/m, D2 – 5.0 dS/m, D3 – 7.5 dS/m, D4 – 10.0 dS/m, D5 – 12.5 dS/m) through observation of morphological and physiological characters. Based on relative performance of yield contributing characters and nutrient contents of leaves and stem, Binachinabadam-3 emerged to be a tolerant variety and based on root shoot characters, Dacca-1 appeared as tolerant while Zhingabadam always performed as sensitive variety. Salinity treatments had most adverse effects at flowering stage and followed the trend of sensitivity as flowering stage > vegetative stage> pre sowing stage > pod filling stage. All varieties were found to be tolerant up to salinity level of 7.5 dS/m.
Farzad Aslani, A. Shukor Juraimi, M. Saiful Ahmad-Hamdani, M. Amirul Alam, M. Mahmudul Hasan, F. S. Golestan Hashemi, and Mohammad Bahram
Springer Science and Business Media LLC
Shah Zaman, Shuiqingqing Hu, Md. Amirul Alam, Hongmei Du, and Shengquan Che
Elsevier BV