Dr. Manoj Kumar

@online.gndu.ac.in

Scholar, Department of Microbiology
Guru Nanak Dev University



                 

https://researchid.co/dr.manojkumar

During my doctorate degree, my research is focused on bioremediation of organic pollutants and heavy metals using bacteria (aerobic and anaerobic) and metagenomics studies of polluted sites. This research experience has equipped me with a deep understanding of the microbiology field, as well as strong analytical and problem-solving skills. During my research I have worked on various instruments such as UV-Vis Spectroscopy, PCR, Ultra-sonicator, Gel-electrophoresis, SDS-PAGE, Lyophilizer, Fermentor, Rotary-evaporator, Gel-Doc, Anaerobic Chamber, Bioreactor development for degradation studies, HPLC,GC, LC-MS, XRD, SEM-EDS and AAS etc. Identification and characterization of microbes using NCBI, EzBioCloud and MG-RAST etc.

All the basic knowledge and techniques of microbiology laboratories.

EDUCATION

Graduation: B.Sc. (hons.) in Microbiology from Department of Microbiology, Shoolini Institute of Life Sciences and Business Management affiliated to Himachal Pradesh University (HPU), Shimla in 2010 with first grade (76 %).
Post-Graduation: M. Sc (hons.) Fermentation and Microbial Technology from Department of Microbiology, Guru Nanak Dev University, Amritsar (Punjab) in 2012 with first grade (75 %).
Qualified ASRB-ICAR NET in Agricultural Microbiology in 2016.
PhD. (Microbiology) degree has been officially conferred having specialization in Microbiology with thesis entitled "Evaluation of Bioremediation Potential of Culturable Bacterial Diversity of Polluted Sites and Assessment of their Unculturable Diversity" from Department of Microbiology, Guru Nanak Dev University (GNDU), Amritsar (Punjab) under the guidance of Prof (Dr.) Harvinder Singh Saini, Department of Microbiology, Dean-Faculty of Life Sciences, Guru Nanak Dev University, Amritsar (Punjab).

RESEARCH, TEACHING, or OTHER INTERESTS

Microbiology, Molecular Biology, Applied Microbiology and Biotechnology, Immunology and Microbiology

27

Scopus Publications

39

Scholar Citations

3

Scholar h-index

2

Scholar i10-index

Scopus Publications

  • Environmental impacts of air pollution and its abatement by plant species: A comprehensive review
    Akshay Kumar Singh, Manoj Kumar, Kuldeep Bauddh, Ajai Singh, Pardeep Singh, Sughosh Madhav, and Sushil Kumar Shukla

    Springer Science and Business Media LLC

  • Statistical and spectral analysis of wind over a strategic location
    Nishi Srivastava, Jaya Divyam, Upamanyu Ghosh, Madhu Priya, Nisheeth Saxena, and Manoj Kumar

    IWA Publishing
    Abstract In the present work, we explored the inherent characteristics of the wind over a complex terrain site ‘Ranchi’ situated near a strategic location of the monsoon trough with various mathematical and statistical tools, i.e., time-series analysis, Fast Fourier transform (FFT), FFT coefficients, wavelet decomposition, and Weibull distribution. The time-series analysis showed a rapid day-to-day variability with a seasonal variation with a peak during summer. Fourier coefficients were concentrated for the winter/post-monsoon, indicating lower wind conditions, while wide spreads of the points indicate agility, i.e., high wind during the summer. The spectral features obtained using FFTs infer that wind has a prominent peak at a frequency f=0.00106724 (day−1) and f=0.00266809 (day−1). The power spectrum and wavelet decomposition show that the prominent frequencies correspond to yearly, eight, six, and four months. Weibull probability density function, cumulative probability distributions, and probability profiles are studied. Results show that the Weibull distribution function reasonably models the probability distribution of daily wind speed. Weibull scale parameter varied between 0.26 and 1.33 m/s, and the shape parameter ranged between 1.09 and 2.88. Results from various analyses indicate that the seasonal variation of wind speed over Ranchi is mainly associated with the development of monsoon trough over the site.

  • Harnessing phytomicrobiome signals for phytopathogenic stress management
    Akanksha Sharma, Meenakshi Raina, Deepak Kumar, Archana Singh, Samira Chugh, Shalu Jain, Manoj Kumar, and Anjana Rustagi

    Springer Science and Business Media LLC

  • Soil Enzymes and Their Role in Soil Health Improvement
    Rini Rahul, Pallavi Sharma, Ashutosh Singh, Joginder Singh, and Manoj Kumar

    Springer Nature Singapore

  • Role of microorganism in phytoremediation of mine spoiled soils
    Ankit Abhilash Swain, Neha Dwivedi, Kuldeep Bauddh, and Manoj Kumar

    Elsevier

  • Phytoremediation of heavy metal contaminated soil in association with arbuscular mycorrhizal fungi
    Sonal Dixit, Preetanshika Tracy, Neha Vishnoi, Ankit Abhilash Swain, Kuldeep Bauddh, and Manoj Kumar

    Elsevier

  • Screening for probiotic attributes of lactic acid bacteria isolated from human milk and evaluation of their anti-diabetic potentials
    Manoj Kumar, Sukumaran Karthika, Nair Anjitha, Perumal Varalakshmi, and Balasubramaniem Ashokkumar

    Informa UK Limited
    ABSTRACT Lactic acid bacteria (LAB) have gained much attention recently due to their probiotic attributes with anti-ACE and anti-glycation potentials targeting management of diabetes. This study was designed to isolate LAB from human milk and to evaluate their potential for inhibitory activities on ACE and AGEs formation using in vitro assay models. Among 12 LAB isolates, many strains exhibited strong probiotic attributes including tolerance to low pH, high salt, gastric, and intestinal juices with higher cell adhesion. Further, cell-free extracts of LAB tested for in vitro anti-ACE and anti-AGEs activities evidenced Lacticaseibacillus casei MKU1, Lacticaseibacillus paracasei MKU2, Lactiplantibacillus pentosus MKU3, L. paracasei MKU7, L. delbrueckii GERU3 and Lactobacillus delbrueckii MKU10 as strong ACE and AGEs inhibitors. Subsequently, Principal Component Analysis showed a positive correlation with anti-ACE (p ≤ .01) and anti-glycation (p ≤ .05) activity of LAB. Overall, this study suggests that six Lactobacillus isolates of human milk with superior probiotic attributes looks promising as potential probiotic strains.

  • Microbial Cell Factories for the Biosynthesis of Vanillin and Its Applications
    Sukumaran Karthika, Manoj Kumar, Santhalingam Gayathri, Perumal Varalakshmi, and Balasubramaniem Ashokkumar

    Wiley

  • Ecological restoration of coal fly ash–dumped area through bamboo plantation
    Raushan Kumar, Mohan Manu Thangaraju, Manoj Kumar, Sanjog Tarachand Thul, Vimal Chandra Pandey, Swati Yadav, Lal Singh, and Sunil Kumar

    Springer Science and Business Media LLC

  • Exploring spatial and temporal trends of diurnal temperature range in the region of the Subarnarekha river basin India
    Shashank Shree, Manoj Kumar, and Ajai Singh

    Springer Science and Business Media LLC

  • Preface



  • An insight in bacteriophage based biosensors with focus on their detection methods and recent advancements
    Simranjeet Singh, Daljeet Singh Dhanjal, Sonali, Siddharth Thotapalli, Vijay Kumar, Shivika Datta, Vineet Kumar, Manoj Kumar, and Joginder Singh

    Elsevier BV

  • Herbicide glyphosate: Toxicity and microbial degradation
    Simranjeet Singh, Vijay Kumar, Jatinder Pal Kaur Gill, Shivika Datta, Satyender Singh, Vaishali Dhaka, Dhriti Kapoor, Abdul Basit Wani, Daljeet Singh Dhanjal, Manoj Kumar,et al.

    MDPI AG
    Glyphosate is a non-specific organophosphate pesticide, which finds widespread application in shielding crops against the weeds. Its high solubility in hydrophilic solvents, especially water and high mobility allows the rapid leaching of the glyphosate into the soil leading to contamination of groundwater and accumulation into the plant tissues, therefore intricating the elimination of the herbicides. Despite the widespread application, only a few percentages of the total applied glyphosate serve the actual purpose, dispensing the rest in the environment, thus resulting in reduced crop yields, low quality agricultural products, deteriorating soil fertility, contributing to water pollution, and consequently threatening human and animal life. This review gives an insight into the toxicological effects of the herbicide glyphosate and current approaches to track and identify trace amounts of this agrochemical along with its biodegradability and possible remediating strategies. Efforts have also been made to summarize the biodegradation mechanisms and catabolic enzymes involved in glyphosate metabolism.

  • Evaluating metabolic potential of Thauera sp. M9 for the transformation of 4-chloroaniline (4-CA)
    Manoj Kumar, Rohit Mahajan, and Harvinder Singh Saini

    Elsevier BV

  • Detection of change over land use and land cover in Coimbatore urban area


  • Metagenomic analysis of the fecal microbiome of an adult elephant reveals the diversity of CAZymes related to lignocellulosic biomass degradation
    Shaik Jakeer, Mahendra Varma, Juhi Sharma, Farnaz Mattoo, Dinesh Gupta, Joginder Singh, Manoj Kumar, and Naseem A. Gaur

    Springer Science and Business Media LLC

  • Hippophae rhamnoides L. rhizobacteria exhibit diversified cellulase and pectinase activities
    Pooja Bhadrecha, Madhu Bala, Yogender Pal Khasa, Anfal Arshi, Joginder Singh, and Manoj Kumar

    Springer Science and Business Media LLC


  • Reduction of hexavalent chromium (VI) by indigenous alkaliphilic and halotolerant Microbacterium sp. M5: comparative studies under growth and nongrowth conditions
    M. Kumar and H.S. Saini

    Oxford University Press (OUP)
    To evaluate hexavalent chromium (Cr (VI)) reduction potential of indigenous isolate M5, under growing and nongrowing conditions.

  • Inheritance pattern of genes for morpho-physiological and yield traits in wheat (triticum aestivum L.)
    J. Kumar, A. Kumar, M. Kumar, S.K. Singh, and L. Singh

    Springer Science and Business Media LLC
    In order to study the inheritance pattern of morpho-physiological traits in bread wheat, a 10×10 diallel cross, excluding reciprocals was made and grown in a randomized complete block design (RCBD) with three replications. Observations were recorded on Days to 75% flowering (DF), Days to maturity (DM), Duration of reproductive phase (DRP), Plant height (cm) (PH), Effective tiller/plant (TLS), No. of spikelets per spike (SLS), No. of grains per spike (GS), Grain weight per spike (g) (GW), Spike length (cm) (SL), Biological yield per plant (g) (BY), Harvest index (%) (HI), 1000-Grain weight (g) (TGW), Spike density (SD), Canopy temperature depression (°C) (CTD), Chlorophyll intensity (%) (CI), Chlorophyll fluorescence (Fv/Fm) (CF), Protein content (%) (PC), Grain yield per plant (g) (GY). Highly significant differences were observed among the genotypes for all traits. The resulted 45 F1s and their F2s used for study the nature of gene for grain yield and its contributing traits in bread wheat. The result indicated that considerable gene action and average degree of dominance respond to achieving significant result for grain yield and its component traits. In both the generations F1s and F2s, grain yield per plant (g) was governed by non-additive gene action based on combining ability analysis, (σ2 g/σ2 s)0.5 [GCA and SCA variance ratio] and (H1/D)0.5 [Degree of dominance] were exhibited over dominance type average degree of dominance for grain yield and its component traits in both generations. Genetic analyses of the traits confirm the involvement of both additive and non-additive gene effects in governing the inheritance.

  • Microbiome in Plant Health and Disease: Challenges and Opportunities
    Vivek Kumar, R. Prasad, M. Kumar and D. Choudhary

    Springer Singapore

  • Phytobiont and ecosystem restitution
    Vivek Kumar, Manoj Kumar and R. Prasad

    Springer Singapore

  • In silico approach for sustainable agriculture
    D. Choudhary, Manoj Kumar, R. Prasad and Vivek Kumar

    Springer Singapore

  • Solar radiation over four cities of India: Trend analysis using Mann-Kendall Test


RECENT SCHOLAR PUBLICATIONS

  • Novel approaches for achieving bioremediation and sustainable management of chromium contaminated wastewater
    HSS Manoj Kumar
    Wastewater Treatment - Past and Future Perspectives 1, 1-26 2024

  • Origin and Management of Inorganic and Organic Contaminants
    S Dhiman, S Kumar, M Kumar, G Kumar
    Management and Mitigation of Emerging Pollutants 1, 21-67 2023

  • Exploring the role of microbes for the management of persistent organic pollutants
    M Kumar, SK Saggu, P Pratibha, SK Singh, S Kumar
    Journal of Environmental Management 344, 118492 2023

  • Synergistic and additive interactions of Shewanella sp., Pseudomonas sp. and Thauera sp. with chlorantraniliprole and emamectin benzoate for controlling Spodoptera litura
    HSSSK Sunaina Sarkhandia, Geetika Sharma, Rohit Mahajan, Satish Koundal ...
    Scientific Reports 13, 14648 2023

  • Insecticidal, genotoxic and biochemical effects of Shewanella sp. (SS4) and Thauera sp. (M9) on Spodoptera litura
    SK Sunaina Sarkhandia, Sarita Devi, Geetika Sharma, Manoj Kumar, Satish ...
    Journal of Applied Entomology, 1-14 2023

  • Evaluating metabolic potential of Thauera sp. M9 for the transformation of 4-chloroaniline (4-CA)
    M Kumar, R Mahajan, HS Saini
    Biocatalysis and agricultural biotechnology 29, 101768 2020

  • Reduction of hexavalent chromium (VI) by indigenous alkaliphilic and halotolerant Microbacterium sp. M5: comparative studies under growth and nongrowth
    M Kumar, HS Saini
    Journal of applied microbiology 127 (4), 1057-1068 2019

MOST CITED SCHOLAR PUBLICATIONS

  • Reduction of hexavalent chromium (VI) by indigenous alkaliphilic and halotolerant Microbacterium sp. M5: comparative studies under growth and nongrowth
    M Kumar, HS Saini
    Journal of applied microbiology 127 (4), 1057-1068 2019
    Citations: 18

  • Evaluating metabolic potential of Thauera sp. M9 for the transformation of 4-chloroaniline (4-CA)
    M Kumar, R Mahajan, HS Saini
    Biocatalysis and agricultural biotechnology 29, 101768 2020
    Citations: 15

  • Exploring the role of microbes for the management of persistent organic pollutants
    M Kumar, SK Saggu, P Pratibha, SK Singh, S Kumar
    Journal of Environmental Management 344, 118492 2023
    Citations: 4

  • Synergistic and additive interactions of Shewanella sp., Pseudomonas sp. and Thauera sp. with chlorantraniliprole and emamectin benzoate for controlling Spodoptera litura
    HSSSK Sunaina Sarkhandia, Geetika Sharma, Rohit Mahajan, Satish Koundal ...
    Scientific Reports 13, 14648 2023
    Citations: 1

  • Insecticidal, genotoxic and biochemical effects of Shewanella sp. (SS4) and Thauera sp. (M9) on Spodoptera litura
    SK Sunaina Sarkhandia, Sarita Devi, Geetika Sharma, Manoj Kumar, Satish ...
    Journal of Applied Entomology, 1-14 2023
    Citations: 1