Xuemeng ZHANG
@tongji.edu.cn
State Key Laboratory of Pollution Control and Resource Reuse
Tongji University
EDUCATION
2021.03 - Present, Tongji University, Ph.D. Student
· Environmental Engineering
· GPA: 90.80
· Supervisor: Prof., Yinguang Chen
2019.09 - 2021.02, Tongji University, Master Student
· Environmental Engineering
· GPA: 89.30
· Supervisor: Prof., Yinguang Chen
2015.09 - 2019.06, Nanjing Agricultural University, Bachelor of Engineering
· Environmental Engineering
· GPA: 91.09, Top 5%
· Supervisor: Prof., Guanyu Zheng
RESEARCH, TEACHING, or OTHER INTERESTS
Environmental Engineering, Water Science and Technology, Pollution, Waste Management and Disposal
Scopus Publications
Scopus Publications
Meirou Wu, Yanan Xu, Chunxia Zhao, Haining Huang, Chao Liu, Xu Duan, Xuemeng Zhang, Guohua Zhao, and Yinguang Chen
Elsevier BV
Jing Zhou, Dapeng Li, Xuemeng Zhang, Chao Liu, and Yinguang Chen
Elsevier BV
Chunxia Zhao, Xu Duan, Chao Liu, Haining Huang, Meirou Wu, Xuemeng Zhang, and Yinguang Chen
American Chemical Society (ACS)
Antibiotics often coexist with other pollutants (e.g., nitrate) in an aquatic environment, and their simultaneous biological removal has attracted widespread interest. We have found that sulfamethoxazole (SMX) and nitrate can be efficiently removed by the coculture of a model denitrifier (Paracoccus denitrificans, Pd) and Shewanella oneidensis MR-1 (So), and SMX degradation is affected by NADH production and electron transfer. In this paper, the mechanism of a coculture promoting NADH production and electron transfer was investigated by proteomic analysis and intermediate experiments. The results showed that glutamine and lactate produced by Pd were captured by So to synthesize thiamine and heme, and the released thiamine was taken up by Pd as a cofactor of pyruvate and ketoglutarate dehydrogenase, which were related to NADH generation. Additionally, Pd acquired heme, which facilitated electron transfer as heme, was the important composition of complex III and cytochrome c and the iron source of iron sulfur clusters, the key component of complex I in the electron transfer chain. Further investigation revealed that lactate and glutamine generated by Pd prompted So chemotactic moving toward Pd, which helped the two bacteria effectively obtain their required substances. Obviously, metabolite cross-feeding promoted NADH production and electron transfer, resulting in efficient SMX biodegradation by Pd and So in the presence of nitrate. Its feasibility was finally verified by the coculture of an activated sludge denitrifier and So.
Chen Wang, Xuemeng Zhang, Guohua Zhao, and Yinguang Chen
Elsevier BV
Chao Liu, Xuemeng Zhang, Chuang Chen, Yue Yin, Guohua Zhao, and Yinguang Chen
American Chemical Society (ACS)
Acetotrophic methanogens' dysfunction in anaerobic digestion under ammonia pressure has been widely concerned. Lipids, the main cytomembrane structural biomolecules, normally play indispensable roles in guaranteeing cell functionality. However, no studies explored the effects of high ammonia on acetotrophic methanogens' lipids. Here, a high-throughput lipidomic interrogation deciphered lipid reprogramming in representative acetoclastic methanogen (Methanosarcina barkeri) upon high ammonia exposure. The results showed that high ammonia conspicuously reduced polyunsaturated lipids and longer-chain lipids, while accumulating lipids with shorter chains and/or more saturation. Also, the correlation network analysis visualized some sphingolipids as the most active participant in lipid-lipid communications, implying that the ammonia-induced enrichment in these sphingolipids triggered other lipid changes. In addition, we discovered the decreased integrity, elevated permeability, depolarization, and diminished fluidity of lipid-supported membranes under ammonia restraint, verifying the noxious ramifications of lipid abnormalities. Additional analysis revealed that high ammonia destabilized the structure of extracellular polymeric substances (EPSs) capable of protecting lipids, e.g., declining α-helix/(β-sheet + random coil) and 3-turn helix ratios. Furthermore, the abiotic impairment of critical EPS bonds, including C-OH, C═O-NH-, and S-S, and the biotic downregulation of functional proteins involved in transcription, translation, and EPS building blocks' supply were unraveled under ammonia stress and implied as the crucial mechanisms for EPS reshaping.
Chao Liu, Yue Yin, Chuang Chen, Xuemeng Zhang, Jing Zhou, Qingran Zhang, and Yinguang Chen
MDPI AG
Medium chain carboxylic acids (MCCAs, e.g., caproic acid, caprylic acid, etc.) with 6–12 carbon atoms are valuable platform chemicals produced from organic waste via microbial chain elongation metabolism named as reversed β-oxidation and fatty acid-biosynthesis cyclical pathway. Recently, many articles reported that electricity could not only serve as the external electron donor and provide the reduction equivalent required for chain elongation but also regulate the microbiome structure and metabolic behaviors to promote MCCAs formation. Electricity-steering MCCAs bioproduction has become an appealing technique to valorize low-value organic waste, paving an alternative pathway for net-zero carbon emission energy systems and sustainable socio-economic development. However, the MCCAs’ bioproduction from organic waste steered by electric field has not been comprehensively reviewed. From a systematical analysis of publicly available literature, we first covered the basic working principle, fermentation architecture, functional microflora, and metabolic pathway of MCCAs production driven by electricity. The strategies of substrate modulation, applied voltage/current regulation, electrode optimization, and microbial cooperation and stimulation for boosting electricity-driven MCCAs bioproduction are then scrutinized and extensively discussed. Ultimately, the pressing knowledge gaps and the potential path forward are proposed to provide pointers for consistently higher MCCAs yield and the transition from laboratory to market.
Xinyun Fan, Xuemeng Zhang, Guohua Zhao, Xin Zhang, Lei Dong, and Yinguang Chen
Springer Science and Business Media LLC
Meng Wang, Xuemeng Zhang, Haining Huang, Zhiyi Qin, Chao Liu, and Yinguang Chen
American Chemical Society (ACS)
During proteinaceous waste valorization to produce volatile fatty acids (VFAs), protein needs to be hydrolyzed to amino acids (AAs), but the effects of the configuration of AAs on their biotransformation and VFA production have not been investigated. In this study, more residual d-AAs than their corresponding l-AAs were observed after VFAs were produced from kitchen waste in a pilot-scale bioreactor. For all AAs investigated, the VFA production from d-AAs was lower than that from corresponding l-AAs. The metagenomics and metaproteomics analyses revealed that the l-AA fermentation system exhibited greater bacterial chemotaxis and quorum sensing (QS) than d-AAs, which benefited the establishment of functional microorganisms (such as Clostridium, Sedimentibacter, and Peptoclostridium) and expression of functional proteins (e.g., substrate transportation cofactors, l-AA dehydrogenase, and acidogenic proteins). In addition, d-AAs need to be racemized to l-AAs before being metabolized, and the difference of VFA production between d-AAs and l-AAs decreased with the increase of racemization activity. The findings of the AA configuration affecting bacterial chemotaxis and QS, which altered microorganism communities and functional protein expression, provided a new insight into the reasons for higher l-AA metabolism than d-AAs and more d-AAs left during VFA production from proteinaceous wastes.
Xuemeng Zhang, Tong Yu, Chao Liu, Xinyun Fan, Yang Wu, Meng Wang, Chunxia Zhao, and Yinguang Chen
Elsevier BV
Chuang Chen, Xuemeng Zhang, Chao Liu, Yang Wu, Guanghong Zheng, and Yinguang Chen
Elsevier BV
Yuexi Chen, Xuemeng Zhang, and Yinguang Chen
Elsevier BV
Xuemeng Zhang, Chao Liu, Yuexi Chen, Guanghong Zheng, and Yinguang Chen
Springer Science and Business Media LLC