Tannic acid attenuates glyphosate-based herbicide-induced dopaminergic neurotoxicity associated with motor dysfunction in mice Patrick Oluwole Abolarin, Bamidele Victor Owoyele Frontiers in Behavioral Neuroscience, 2026 Introduction Glyphosate, a widely used herbicide, has gained attention due to its potential link to neurobehavioral and dopaminergic dysfunctions. Data on interventions against glyphosate-induced neurotoxicity are limited. Hence, the neuroprotective role of tannic acid (TA), a polyphenolic anti-inflammatory and antioxidant agent, was investigated in mice chronically exposed to glyphosate-based herbicide (GBH). Methods Male Swiss mice were randomly allocated into six groups ( n = 8) and received daily oral gavages of specific solutions that were prepared daily: Control (distilled water 1 mL/kg body weight), GBH (500 mg/kg body weight), Pre-TA + GBH (TA 50 mg/kg body weight, pre-treated, then GBH-exposed), TA + GBH (TA 50 mg/kg body weight and GBH-co-treatment), Pre-AA + GBH [ascorbic acid (AA) 100 mg/ kg body weight, pre-treated, then GBH-exposed], and AA + GBH (AA 100 mg/ kg body weight and GBH-co-treatment). Motor function tests, biochemical, and histological analyses of the midbrain were performed 6 weeks post-treatment. Results TA significantly inhibited GBH-induced motor dysfunction. As compared to the GBH group, TA treatments significantly (p < 0.0001) decreased midbrain malondialdehyde (MDA), TNF-α, IL-1β, and IL-6 levels. TA treatments increased significantly ( p < 0.0001) the concentrations of dopamine, the activities of catalase (CAT), glutathione peroxidase (GPx), and superoxide dismutase (SOD) enzymes in the midbrain relative to the GBH group. These effects were similar to those of the control and AA-treated mice. Conclusively, TA ameliorated GBH-induced motor dysfunction in mice and attenuated associated midbrain oxidative stress, inflammatory responses, and dopaminergic alterations. Discussion These findings are suggestive of the neuroprotective effects of TA against environmental toxicant-induced neurotoxicity.
Effectiveness of Bacopa Monnieri (Brahmi) in the management of schizophrenia: a systematic review Gideon Opeyemi Ayilara, Bamidele Victor Owoyele Nutritional Neuroscience, 2025 Schizophrenia is a psychotic disorder affecting approximately 0.32% of the global population. Despite advancements in pharmacological treatments, many patients with schizophrenia continue to experience significant impairments, and approximately one-third of these patients do not respond to antipsychotic drugs. However, various studies have demonstrated the potential benefits of herbs in managing schizophrenia due to the diverse biological activities of phytochemicals, including neuroprotective activity, anti-oxidant potential, modulation of neurotransmission, and anti-inflammatory activity. Bacopa monnieri (Brahmi) is a widely studied herb used in the treatment of the central nervous system. This study conducted a systematic review to determine the effectiveness of Brahmi in managing schizophrenia. PubMed, Scopus, Web of Science, and Cochrane databases were searched between February and March, 2024. A total of 103 articles were found, with only 9 studies meeting the eligibility criteria. Data analysis was done by using themes. The review found that Brahmi could reverse positive, negative, and cognitive symptoms of schizophrenia. It does this by changing the glutamatergic pathway and GABAergic transmission, lowering MDA levels, raising GSH levels, slowing down the activity of acetylcholinesterase (AchE), and maintaining the density of neurones. It is recommended that additional research elucidating the effects of Brahmi in other models of schizophrenia and the possible mechanisms of action be conducted.
Tannic acid inhibits pain mediators, inflammation and oxidative stress in mice exposed to glyphosate-based herbicide Patrick Oluwole Abolarin, Bamidele Victor Owoyele Environmental Analysis Health and Toxicology, 2024 Chronic exposure to glyphosate-based herbicide (Gly) has been associated with neurological disorders. Tannic acid (TA) is an antioxidant with attenuating action against neuroinflammation-associated conditions. This study evaluated the effect of Gly on pain perception alongside antinociceptive and anti-inflammatory actions of TA in Gly-exposed mice. Male Swiss mice were randomly divided into six groups (n=8): control (distilled water 0.2 ml/kg), Gly (Gly 500 mg/kg), Pre-TA + Gly (TA 50 mg/kg pre-treatment, afterwards Gly-administered), TA + Gly (TA 50 mg/kg and Gly co-administered), Pre-AA + Gly (ascorbic acid (AA) 10 mg/kg pre-treatment, afterwards Gly-administered), and AA + Gly (AA 10 mg/kg and Gly co-administered). Mechanical, thermal, and chemical pain were evaluated six weeks post vehicle/drugs administrations orally, followed by brain biochemical measurements. TA treatment alleviated Gly-induced hyperalgesia in similar version to the values of control and AA groups by increasing significantly (p < 0.05) nociceptive thresholds. Moreover, TA-treatment significantly decreased malondialdehyde (MDA) and pro-inflammatory cytokines (TNF-α, IL-1β, and IL-6) levels, significantly increased anti-inflammatory cytokines (IL-10, IL-4, and TGF-1β) levels, and antioxidant enzymes, catalase (CAT), glutathione peroxidase (GPx), and superoxide dismutase (SOD) activities compared to Gly-treated mice (p < 0.05). Conclusively, TA treatment exerted antinociceptive and anti-inflammatory actions, possibly through its antioxidant and anti-inflammatory actions in Gly-exposed mice. Notably, TA pre-treatment showed a better response than TA and Gly co-administration. We propose the potential neuroprotective and ameliorative functions of TA in Gly-induced hyperalgesia. This merits further clinical research into protective roles of TA against pesticide-related conditions.
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