Elisabete José Vicente

@microbiologia.icb.usp.br

Biomedical Science Institute/University of São Paulo
University of São Paulo

Elisabete José Vicente


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https://researchid.co/bevicent

RESEARCH, TEACHING, or OTHER INTERESTS

Microbiology, Biotechnology, Molecular Biology
18

Scopus Publications

351

Scholar Citations

11

Scholar h-index

15

Scholar i10-index

Scopus Publications

  • Expression of synthetic phytochelatin ec20 in e. Coli increases its biosorption capacity and cadmium resistance
    Cleide Barbieri de Souza, Elisabete José Vicente
    Bioscience Journal, 2020
    In this study E. coli recombinant clones that express the EC20 synthetic phytochelatin intracellularly were constructed. The increasement of Cd2+ biosorption capacity, and, also, the increasement of resistance to this toxic metal were analyzed. A gene that encodes the synthetic phytochelatin EC20 was synthesized in vitro. The EC20 synthetic gene was amplified by PCR, inserted into the DNA cloning vectors pBluescript®KS+ and pGEM®-TEasy, and also into the expression vectors pTE [pET-28(a)® derivative] and pGEX-T4-2®. The obtained recombinant plasmids were employed for genetic transformation of E. coli: pBsKS-EC20 and pGEM-EC20, they were introduced into DH10B and DH5α strains, similarly to pTE-EC20 and pGEX-EC20 that were introduced into BL21 strain. The EC20 expression was confirmed by SDS-PAGE analysis. The recombinant clones’ resistances to Cd2+ were determined by MIC analyses. The MIC for Cd2+ of DH10B/pBKS-EC20 and DH10B/pGEM-EC20 were 2.5 mM (EC20 induced), and 0.312 mM (EC20 repressed); respectively, 16 and 2 times higher than the control DH10B/pBsKS (0.156 mM). The MIC for Cd2+ of BL21/pTE-EC20 was 10.0 mM (EC20 induced) and 2.5 mM (EC20 repressed), compared with the control BL21/pTE which was only 1.25 mM. Analysis of ICP-AES showed that BL21/pGEX-EC20, after growth on the condition of EC20 expression, absorbed 37.5% of Cd2+, and even when cultured into the non-induction condition of EC20 expression, it absorbed 11.5%. These results allow the conclusion that recombinant E. coli clones expressing the synthetic phytochelatin EC20 show increased capacity for Cd2+ biosorption and enhanced resistance to this toxic ion.
  • Synthetic phytochelatin surface display in cupriavidus metallidurans CH34 for enhanced metals bioremediation
    Ronaldo Biondo, Felipe Almeida da Silva, Elisabete José Vicente, Jorge Eduardo Souza Sarkis, Ana Clara Guerrini Schenberg
    Environmental Science and Technology, 2012
    This work describes the effects of the cell surface display of a synthetic phytochelatin in the highly metal tolerant bacterium Cupriavidus metallidurans CH34. The EC20sp synthetic phytochelatin gene was fused between the coding sequences of the signal peptide (SS) and of the autotransporter β-domain of the Neisseria gonorrhoeae IgA protease precursor (IgAβ), which successfully targeted the hybrid protein toward the C. metallidurans outer membrane. The expression of the SS-EC20sp-IgAβ gene fusion was driven by a modified version of the Bacillus subtilis mrgA promoter showing high level basal gene expression that is further enhanced by metal presence in C. metallidurans. The recombinant strain showed increased ability to immobilize Pb(2+), Zn(2+), Cu(2+), Cd(2+), Mn(2+), and Ni(2+) ions from the external medium when compared to the control strain. To ensure plasmid stability and biological containment, the MOB region of the plasmid was replaced by the E. coli hok/sok coding sequence.
  • Autolytic Mycobacterium leprae Hsp65 fragments may act as biological markers for autoimmune diseases
    Carolina Angélica Parada, Fernanda Portaro, Eliana Blini Marengo, Clécio Fernando Klitzke, Elisabete José Vicente, Marcella Faria, Osvaldo Augusto Sant’Anna, Beatriz Lieblich Fernandes
    Microbial Pathogenesis, 2011
  • Endostatin- and interleukin-2-expressing retroviral bicistronic vector for gene therapy of metastatic renal cell carcinoma
    Flávia Gomes de Góes Rocha, Fernanda B. Calvo, Karen C. Chaves, Jean P. S. Peron, Rodolfo F. Marques, Tânia R. de Borba, Marina S. Braga, Cleide B. Pereira, Elisabete J. Vicente, Roger Chammas, Nestor Schor, Maria H. Bellini
    Journal of Gene Medicine, 2011
    Metastatic renal cell carcinoma (mRCC) is one of the most treatment‐resistant malignancies. Despite all new therapeutic advances, almost all patients develop resistance to treatment and cure is rarely seen. In the present study, we evaluated the antitumor effect of a bicistronic retrovirus vector encoding both endostatin (ES) and interleukin (IL)‐2 using an orthotopic metastatic RCC mouse model.
  • A metal-repressed promoter from Gram-positive bacillus subtilis is highly active and metal-induced in Gram-negative Cupriavidus metallidurans
    Gabriela Ribeiro-dos-Santos, Ronaldo Biondo, Oeber de Freitas Quadros, Elisabete José Vicente, Ana Clara Guerrini Schenberg
    Biotechnology and Bioengineering, 2010
    A synthetic version of the metal‐regulated gene A (mrgA) promoter from Bacillus subtilis, which in this Gram‐positive bacterium is negatively regulated by manganese, iron, cobalt, or copper turned out to promote high level of basal gene expression that is further enhanced by Co(II), Cd(II), Mn(II), Zn(II), Cu(II), or Ni(II), when cloned in the Gram‐negative bacterium Cupriavidus metallidurans. Promoter activity was monitored by expression of the reporter gene coding for the enhanced green fluorescent protein (EGFP), and cellular intensity fluorescence was quantified by flow cytometry. Expression levels in C. metallidurans driven by the heterologous promoter, here called pan, ranged from 20‐ to 53‐fold the expression level driven by the Escherichia coli lac promoter (which is constitutively expressed in C. metallidurans), whether in the absence or presence of metal ions, respectively. The pan promoter did also function in E. coli in a constitutive pattern, regardless of the presence of Mn(II) or Fe(II). In conclusion, the pan promoter proved to be a powerful tool to express heterologous proteins in Gram‐negative bacteria, especially in C. metallidurans grown upon high levels of toxic metals, with potential applications in bioremediation. Biotechnol. Bioeng. 2010;107: 469–477. © 2010 Wiley Periodicals, Inc.
  • Porous 3-D scaffold of poly-hydroxy-alkanoates for cartilage tissue engineering
    8th World Biomaterials Congress 2008, 2008
  • In vitro effects of gamma radiation from 60Co and 137Cs on plasmid DNA
    F. M. Milian, A. N. Gouveia, M. R. Gual, J. O. Echeimberg, J. D. T. Arruda-Neto, F. Garcia, A. C. G. Schenberg, E. J. Vicente, O. Rodriguez, F. Guzman, A. Deppman
    Journal of Biological Physics, 2007
  • A novel system of genetic transformation allows multiple integrations of a desired gene in Saccharomyces cerevisiae chromosomes
    Odanir Garcia Guerra, Ileana G.S. Rubio, Claudionor Gomes da Silva Filho, Regiane Aparecida Bertoni, Rute Cardoso dos Santos Govea, Elisabete José Vicente
    Journal of Microbiological Methods, 2006
  • Pectinolytic activity secreted by yeasts isolated from fermented citrus molasses
    K.F. Oliveira, L. Malavolta, C.S. Souza, E.J. Vicente, C. Laluce
    Journal of Applied Microbiology, 2006
    Aims: The aim of this study was to obtain improved strains of pectinolytic yeasts adapted to the conditions of an industrial fermentation process, which was continuously operated to convert citrus molasses into ethanol.
  • Radiation interaction with DNA
    A. Deppman, J.O. Echeimberg, A.N. Gouveia, J.D.T. Arruda-Neto, F.M. Milian, N. Added, M.E. Camargo, F. Guzman, O.A.M. Helene, V.P. Likhachev, O. Rodriguez, A.C.G. Schenberg, V. Vanin, E.J. Vicente
    Brazilian Journal of Physics, 2004
    We developed a model to describe the radiation-DNA interaction in water solution. The model is based on some important aspects of the Bethe Theory for the ionizing process, and on the assumption that the direct interaction between the incident particle and the DNA molecule is negligible in comparison with the indirect effect. This assumption can always be true under controlled conditions. The results show that the number of damages induced by radiation depends on the accumulated dose, on parameters related only to the medium properties, and on the DNA structure, but not on the incident particle. A few applications of the model are discussed, and results are successfully compared with experimental data. New experiments are proposed.
  • Evaluation of alternative reporter genes for the yeast two-hybrid system
    Genetics and Molecular Research, 2003
  • Phenotypic characterization of Trichophyton rubrum isolates from two geographic locations in Brazil
    J.I. Dos Santos, E.J. Vicente, C.R. Paula, W. Gambale
    European Journal of Epidemiology, 2001
  • Studies on polyhydroxyalkanoate (PHA) accumulation in a PHA synthase I-negative mutant of Burkholderia cepacia generated by homogenotization
    M de Andrade Rodrigues
    FEMS Microbiology Letters, 2000
  • Antimicrobial resistance patterns of Haemophilus influenzae isolated from patients with meningitis in São Paulo, Brazil
    S.T. Casagrande, E.J. Vicente, I.M. Landgraf, A.M.M. Kobata
    Brazilian Journal of Medical and Biological Research, 2000
  • Further characterization of the yeast pso4-1 mutant: Interaction with rad51 and rad52 mutants after photoinduced psoralen lesions
    Marcos Antonio de Morais, Elisabete José Vicente, Jela Brozmanova, Ana Clara Guerrini Schenberg, João Antonio Pegas Henriques
    Current Genetics, 1996
  • Immunogenic properties of the M. leprae recombinant 18-kDa antigen purified from Saccharomyces cerevisiae; Enhancement of delayed-type hypersensitivity after gamma-irradiation
    International Journal of Leprosy, 1995
  • Expression of the 18kDa protein of Mycobacterium leprae in Saccharomyces cerevisiae
    João Renato Rebello Pinho, Philip J. Barr, Elizabete José Vicente, Ana Clara Schenberg
    Biotechnology Letters, 1994
  • Isolation of Trypanosoma cruzi DNA fragments which function as ARS elements in Saccharomyces cerevisiae
    Santuza Maria Ribeiro Teixeira, Ana Clara Schenberg Frascino, Elisabete Vicente Galembeck, Maristela de Oliveira Azevedo, Spartaco Astolfi Filho
    Gene, 1986

RECENT SCHOLAR PUBLICATIONS

  • Monomeric Esterase: Insights into Cooperative Behavior, Hysteresis/Allokairy
    TC Vinces, AS de Souza, CF Carvalho, AB Visnardi, RD Teixeira, ...
    Biochemistry 63 (9), 1178-1193 , 2024
    2024
    Citations: 11
  • Biotechnology: use of available carbon sources on the planet to generate alternatives energy
    JAC Junior, EJ Vicente, ETG Marasca, FAG Torres, LMP Moraes, ...
    authorea , 2024
    2024
  • Biotechnology: use of available carbon sources on the planet to generate alternatives energy
    J JAdC, EJ Vicente, ETG Marasca, FAG Torres, LMP Moraes, UA Bones
    2024
  • Antibacterial ability of different activated irrigation after root canal preparation: intratubular analyses
    CK Nabeshima, H Caballero-Flores, EJ Vicente, G Gavini, MEL Machado
    Brazilian Dental Journal 35, e24-5883 , 2024
    2024
    Citations: 3
  • Biotechnology: use of available carbon sources on the planet to generate alternatives energy
    JA da Costa Junior, EJ Vicente, ETG Marasca, FAG Torres, ...
    Research, Society and Development 11 (16), e536111627904-e536111627904 , 2022
    2022
  • Molecular and functional basis of a novel Amazonian Dark Earth Esterase 1 (Ade1) with hysteresis behavior and quorum-quenching activity
    TC Vinces, AS Souza, CF Carvalho, RD Teixeira, BAP Bismara, ...
    bioRxiv, 2020.12. 16.421545 , 2020
    2020
  • Expression of synthetic phytochelatin EC20 in E. coli increases its biodorption capacity and cadmium resistance
    CB Souza, EJ Vicente
    Bioscience Journal 36 (2), 619-627 , 2020
    2020
    Citations: 2
  • Evaluation of ethanol extract of Punica granatum (Pomegranate) peel decrease in viral replication of BoHV-1 in Colorado murine embryos experimentally infected
    EG Palazzi, EM Pituco, EJ Vicente, D Hansen, D Felicio, MS Lima, ...
    2016
  • Construction of stable recombinant industrial yeast strains that secretes glucoamylase and alpha-amylase
    EJ Vicente, OG Guerra, FS Carvalho, RG Oliveira, S Astolfi-Filho, ...
    YEAST 32, S171-S171 , 2015
    2015
  • Construction and characterization of a yeast strain designed for mercury bioremediation
    JP Fuentes Rivera, ACG Schenberg, EJ Vicente
    YEAST 32, S248-S249 , 2015
    2015
  • Avaliação da capacidade de biorremediação de metais tóxicos por microrganismos selecionados
    EJ Vicente, R Biondo, CG Silva Filho, CA Brandt
    Águas Subterrâneas , 2015
    2015
  • Análise do impacto na microbiota de ambientes contaminados pelo escoamento de poluente BTEX oriundo de vazamentos de Postos de Combustíveis
    EJ Vicente, R Biondo, CG Silva Filho, CA Brandt, KA do Amaral Melo, ...
    Águas Subterrâneas , 2015
    2015
  • Avaliação do extrato etanólico de casca de Punica granatum (romã) na diminuição da replicação viral do BoHV-1 Colorado em embriões murinos experimentalmente infectados
    EG Palazzi, EM Pituco, EJ Vicente, D Hansen, JD Felicio, MS Lima, ...
    Arquivos do Instituto Biológico 82, 1-9 , 2015
    2015
  • Isolamento e identificação de bactérias com potencial para realizar biorremediação de cobre.
    KR Hornink
    Universidade de São Paulo , 2015
    2015
  • Avaliação dos produtos naturais na diminuição da replicação viral dos BoHV-1 colorados em embriões murinos infectados experimentalmente.
    EG Palazzi
    Universidade de São Paulo , 2015
    2015
  • Gene, ars-r anchorage cassette, ars-r expression-anchorage cassette, recombinant plasmid, bacterial transgenic lineage, use of said gene, use of said lineage in environmental …
    EJ VICENTE, ACG Schenberg, CA da Silva Parada, R Biondo
    US Patent App. 13/902,477 , 2014
    2014
  • Avaliação da estabilidade genética conferida pelo lócus parB e força de expressão dos promotores deste sistema gênico
    FA Silva, EJ Vicente
    2014
  • Construção e caracterização de uma linhagem de levedura desenhada para biorremediação de mercúrio.
    JPFR Navarro
    Universidade de São Paulo , 2013
    2013
  • Gene, mer-r anchorage cassette, mer-r expression-anchorage cassette, recombinant plasmid, transgenic bacteria line, use of said gene, use of said line in environmental …
    EJ VICENTE, ACG SCHENBERG, CA da Silva PARADA, R BIONDO
    2012
  • Synthetic Phytochelatin Surface Display in Cupriavidus metallidurans CH34 for Enhanced Metals Bioremediation
    R Biondo, FA da Silva, EJ Vicente, JE Souza Sarkis, ACG Schenberg
    Environmental science & technology 46 (15), 8325-8332 , 2012
    2012
    Citations: 56

MOST CITED SCHOLAR PUBLICATIONS

  • Synthetic Phytochelatin Surface Display in Cupriavidus metallidurans CH34 for Enhanced Metals Bioremediation
    R Biondo, FA da Silva, EJ Vicente, JE Souza Sarkis, ACG Schenberg
    Environmental science & technology 46 (15), 8325-8332 , 2012
    2012
    Citations: 56
  • Pectinolytic activity secreted by yeasts isolated from fermented citrus molasses
    KF Oliveira, L Malavolta, CS Souza, EJ Vicente, C Laluce
    Journal of applied microbiology 100 (4), 633-640 , 2006
    2006
    Citations: 43
  • Studies on polyhydroxyalkanoate (PHA) accumulation in a PHA synthase I-negative mutant of Burkholderia cepacia generated by homogenotization
    MF de Andrade Rodrigues, EJ Vicente, A Steinbüchel
    FEMS microbiology letters 193 (1), 179-185 , 2000
    2000
    Citations: 30
  • A novel system of genetic transformation allows multiple integrations of a desired gene in Saccharomyces cerevisiae chromosomes
    OG Guerra, IGS Rubio, CG da Silva Filho, RA Bertoni, ...
    Journal of microbiological methods 67 (3), 437-445 , 2006
    2006
    Citations: 24
  • Further characterization of the yeast pso4-1 mutant: interaction with rad51 and rad52 mutants after photoinduced psoralen lesions
    MA de Morais, EJ Vicente, J Brozmanova, ACG Schenberg, ...
    Current genetics 29 (3), 211-218 , 1996
    1996
    Citations: 22
  • Immunogenic Properties of the M. leprae Recombinant 18-kDa Antigen Purified from Saccharomyces cerevisiae, Enhancement of Delayed-Type Hypersensitivity after Gamma-Irradiation
    JRR Pinho, BA Cardi, HF Andrade, PJ Barr, IC Bathurst, EJ Vicente, ...
    International journal of leprosy and other mycobacterial diseases 63, 381-381 , 1995
    1995
    Citations: 21
  • Antimicrobial resistance patterns of Haemophilus influenzae isolated from patients with meningitis in São Paulo, Brazil
    ST Casagrande, EJ Vicente, IM Landgraf, AMM Kobata
    Brazilian Journal of Medical and Biological Research 33, 295-300 , 2000
    2000
    Citations: 19
  • Evaluation of alternative reporter genes for the yeast two-hybrid system
    AL Starling, JM Ortega, KJ Gollob, EJ Vicente, GM Andrade-Nóbrega, ...
    Genet. Mol. Res 2 (1), 124-135 , 2003
    2003
    Citations: 18
  • In Vitro Effects of Gamma Radiation from 60 Co and 137 Cs on Plasmid DNA
    FM Milian, AN Gouveia, MR Gual, JO Echeimberg, JDT Arruda-Neto, ...
    Journal of biological physics 33 (2), 155-160 , 2007
    2007
    Citations: 16
  • Endostatin‐and interleukin‐2‐expressing retroviral bicistronic vector for gene therapy of metastatic renal cell carcinoma
    FGG Rocha, FB Calvo, KC Chaves, JPS Peron, RF Marques, TR de Borba, ...
    The journal of gene medicine 13 (3), 148-157 , 2011
    2011
    Citations: 12
  • Monomeric Esterase: Insights into Cooperative Behavior, Hysteresis/Allokairy
    TC Vinces, AS de Souza, CF Carvalho, AB Visnardi, RD Teixeira, ...
    Biochemistry 63 (9), 1178-1193 , 2024
    2024
    Citations: 11
  • Autolytic Mycobacterium leprae Hsp65 fragments may act as biological markers for autoimmune diseases
    CA Parada, F Portaro, EB Marengo, CF Klitzke, EJ Vicente, M Faria, ...
    Microbial pathogenesis 51 (4), 268-276 , 2011
    2011
    Citations: 11
  • Radiation interaction with DNA
    A Deppman, JO Echeimberg, AN Gouveia, JDT Arruda-Neto, FM Milian, ...
    Brazilian journal of physics 34 (3a), 958-961 , 2004
    2004
    Citations: 11
  • Phenotypic Characterization of Trichophyton rubrum isolates from two geographic locations in Brazil
    JI Dos Santos, EJ Vicente, CR Paula, W Gambale
    European journal of epidemiology 17 (8), 729-735 , 2001
    2001
    Citations: 11
  • A metal‐repressed promoter from gram‐positive Bacillus subtilis is highly active and metal‐induced in gram‐negative Cupriavidus metallidurans
    G Ribeiro‐dos‐Santos, R Biondo, OF Quadros, EJ Vicente, ...
    Biotechnology and bioengineering 107 (3), 469-477 , 2010
    2010
    Citations: 10
  • Obtenção de um mutante de Alcaligenes eutrophus melhorado geneticamente para a produção do co-polímero polihidroxibutirato-polihidroxivalerato (PHB-PHV)
    DM Sartori, EJ Vicente
    1998
    Citations: 7
  • Expression of the 18kDa protein of Mycobacterium leprae in Saccharomyces cerevisiae
    JRR Pinho, PJ Barr, EJ Vicente, AC Schenberg
    Biotechnology letters 16 (12), 1241-1246 , 1994
    1994
    Citations: 7
  • Cepa mutante de Alcaligenes Eutrophus, cepa transgênica de mutante de Alcaligenes Eutrophus e método de obtenção
    EJ Vicente
    Brazilian patent PI, 9805116-4 , 1998
    1998
    Citations: 5
  • Antibacterial ability of different activated irrigation after root canal preparation: intratubular analyses
    CK Nabeshima, H Caballero-Flores, EJ Vicente, G Gavini, MEL Machado
    Brazilian Dental Journal 35, e24-5883 , 2024
    2024
    Citations: 3
  • Biología celular
    MB Rodriguez, EJ Vicente, AAC Leitao, ACG Schenberg, SOP Costa
    1989
    Citations: 3