Zilda Cristiani Gazim
@professor
Graduate and pos graduate programs
Paranaense University
Zilda Cristiani Gazim Researcher at CNPq level 1D since 2022. She coordinated the lato sensu postgraduate course - Specialization in Handling Pharmaceuticals and Cosmetics, class I (2005-2006); class II (2006-2007) and class III (2007-2008). Since February 2000, he has been part of the faculty of the Universidade Paranaense (UNIPAR) teaching Pharmacotechnics, Cosmetology, Homeopathic Pharmacotechnics, Quality Control, Phytocosmetology and Aromatherapy , Biocomposites and Bioassays. In 2013 she joined the faculty of the Postgraduate Program in Biotechnology Applied to Agriculture - master's and doctoral level at Universidade Paranaense-UNIPAR. In 2021 she joined the faculty of the Graduate Program in Animal Science with an emphasis on bioactive products. Expertise in Chemistry of Natural Products, working in the areas: Essential Oils, Volatile Substances of Plants, chromatographic isolation of biomolecules, chemical identification of natural products, Gas Chromatography and Mass Spectro
EDUCATION
PhD (UEM, Maringá-PR, 2010) and Master's (UEM, Maringá-PR, 2005) in Pharmaceutical Sciences: Area of Concentration: Natural biologically active products from the State University of Maringá ( EMU). Degree in Pharmacy from the Federal University of Rio de Janeiro (UFRJ) Rio de Janeiro-RJ (1988) with qualifications in: Industrial Pharmacist from the Federal University of Rio de Janeiro (UFRJ) Rio de Janeiro-RJ (1989) and Biochemical Pharmacist Analysis of Food by the Federal University of Rio de Janeiro (UFRJ) Rio de Janeiro-RJ (1991). Specialization in Homeopathy by the Hahnemannian Institute of Brazil (IHB) Rio de Janeiro-RJ (1993); Specialization in Pharmacology at Universidade Paranaense (UNIPAR) Umuarama-PR (1999).
RESEARCH, TEACHING, or OTHER INTERESTS
Pharmaceutical Science, Plant Science, Pharmacology, Toxicology and Pharmaceutics, General Pharmacology, Toxicology and Pharmaceutics
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
Kátia C. Oliveira, Lidaiane M. S. S. Franciscato, Suelen S. Mendes, Francielly M. A. Barizon, Daniela D. Gonçalves, Lidiane N. Barbosa, Maria G. I. Faria, Juliana S. Valle, Rhaira F. A. Casalvara, José E. Gonçalves,et al.
MDPI AG
Schinus terebinthifolius Raddi, popularly known as “Pink pepper”, is a plant native to Brazil. The objective of this work was to analyze the chemical composition and the antioxidant and antibacterial potential of essential oils (EOs) from the leaves, fruits and twigs of S. terebinthifolius, aiming for their application in food safety. EOs were obtained by hydrodistillation and the chemical composition was determined by gas chromatography coupled to mass spectrometry. Phenolic compounds were quantified and antioxidant activity was evaluated using three different methods. The antibacterial activity was determined by the broth microdilution method against foodborne bacteria. In the chemical analysis, 22 compounds were identified in the leaves, 13 compounds in the fruits and 37 compounds in the twigs, revealing the presence of the main compounds germacrene D (12.04%, 15.78%, 20,41%), caryophyllene (15.97%, 3.12%, 11.73%), α-pinene (11.6%, 17.16%, 2.99%), β-pinene (5.68%, 43.34%, 5.60%) and γ-gurjunene (16,85%, 3,15%) respectively. EOs showed better antioxidant potential using the β-carotene/linoleic acid method with 40.74, 61.52 and 63.65% oxidation inhibition for leaves, fruits and twigs, respectively. The EO from the leaves showed greater antibacterial potential against Escherichia coli and Staphylococcus aureus with a minimum inhibitory concentration (MIC) of 0.62 mg mL−1, a value lower than the MIC of sodium nitrite (5.00 mg mL−1), the antimicrobial standard synthetic. The activities of pink pepper EOs suggest their potential as a biopreservative in foods.
Felipe Górski, Geysiane Moreira Gerotti, José Eduardo Gonçalves, Zilda Cristiani Gazim, and Hélida Mara Magalhães
Springer Science and Business Media LLC
Ailton da Cruz Melo, Isabela Carvalho dos Santos, Adriane Cordeiro Trevisani, Kariny Aparecida Jardim Rúbio, Ana Luisa Cano, Eloiza Teles Caldart, Juliana Aparecida Mendonça, Renata Olivotto Agostinis, Ranulfo Piau Junior, Zilda Cristiani Gazim,et al.
Cambridge University Press (CUP)
Abstract Objective: The aim of this study was to evaluate how relationships between guardians and domestic animals were established and their possible effects during the COVID-19 pandemic period in Brazil. Methods: Data were collected by completing an online questionnaire. Throughout the national territory, 2002 people completed the questionnaire, while respecting social distancing from January 20, 2021, to March 20, 2021. The inclusion and exclusion criteria were ‘to own pets’ or ‘those who had them during the period of quarantine’ in Brazil. The data obtained were treated qualitatively through discourse analysis and content analysis, while the quantitative data were tabulated by the questionnaire application platform itself. Results: The results showed that 97% of the participants were affected by the coronavirus pandemic in Brazil and that of these, 95.5% stated that their pet was important to overcome and bear bad feelings during the period of social isolation. It was possible to observe through the participants’ reports through the online questionnaire that the relationships with their pets were deepened during the pandemic period, and that these had an important role in overcoming bad emotions caused by social distancing. Conclusion: It was concluded that due to these relationships, behaviors such as anxiety and sadness were reduced and the pets themselves also showed an increase in affective behaviors in relation to their guardians.
Gabriela Catuzo Canônico Silva, Mariane de Almeida Machado, Karina Sakumoto, Rodrigo Sadao Inumaro, José Eduardo Gonçalves, Filipa Mandim, Josiana Vaz, Juliana Silveira do Valle, Maria Graciela Iecher Faria, Suelen Pereira Ruiz,et al.
MDPI AG
Gallesia integrifolia, a notable species in the Atlantic Forest, has been traditionally employed in folk medicine for treating rheumatism, asthma, and worms. This study investigated the cellular antioxidant, antiproliferative, and anti-inflammatory activities of the essential oils (EOs) and crude extracts (CEs) from G. integrifolia flowers, fruits, and leaves. The chemical identification of EOs was performed by GC–MS and CEs by UHPLC–MS. Cellular antioxidant and anti-inflammatory activities were assessed through mouse macrophage cell culture. In addition, the antiproliferative potential was evaluated in gastric, colorectal, breast, and lung tumor cell lines and non-tumor VERO cells. EOs predominantly contained organosulfur compounds in flowers (96.29%), fruits (94.94%), and leaves (90.72%). We found the main compound is 2,2′-Disulfanediyldiethanethiol in the EOs of flowers (47.00%), leaves (41.82%), and fruits (44.39%). Phenolic compounds were identified in CEs. The EOs and CEs demonstrated potential against the tumor cell lines tested (GI50 between 51 and 230 µg/mL). The selectivity index values were greater than 1.0 (1.01 to 3.37), suggesting a relative safety profile. Moreover, the anti-inflammatory activity IC50 ranged from 36.00 to 268 µg/mL, and the cellular oxidation inhibition ranged from 69% to 82%. The results suggest that oils and extracts derived from G. integrifolia have potential for use in various industrial sectors.
Nastassja Kimberlly Lima, Eloisa Schneider da Silva, Rayane Monique Sete da Cruz, Julliane Destro de Lima, Cristian Coelho da Silva, Mayara dos Santos Queiroz, Zilda Cristiani Gazim, José Eduardo Gonçalves, Daisy Leticia Ramirez Monzon, Renan Rizzato Espessato,et al.
Springer Science and Business Media LLC
Rosangela Rumi Sugauara, , Wanessa de Campos Bortolucci, Carla Maria Mariano Fernandez, Marcia Regina Pereira Cabral, Regina Aparecida Correia Goncalves, Maria Helena Sarragiotto, Jose Eduardo Goncalves, Nelson Barros Colauto, Giani Andrea Linde,et al.
MS-Editions
Campomanesia xanthocarpaleaves are a byproduct of fruit production without studies on antioxidant activity. Thus, this study aimed to identify the antioxidant compounds of C. xanthocarpaleaves by ultra-high performance liquid chromatography coupled with electrospray ionization-quadrupole-time of flight-mass spectrometry (UHPLC-ESI/qTOF) and by different in vitroantioxidant methods. The crude extract of C. xanthocarpaleaves had a yield of 15.2% and only five out of 37 fractions of the crude extract had antioxidant activity. The crude extract presented greater antioxidant activity than the isolated fractions. The identified antioxidant compounds were phenolic acids (gallic acid and chlorogenic acid), flavonoids (quercetin and naringenin 7,4’-dimethoxy) and an organic acid (quinic acid). Leaves of C. xanthocarpahave high concentration of antioxidant compounds and it is a promising plant for the development of applications in the food, cosmetic, and pharmaceutical fields. The extraction of antioxidant compounds can add value to the productive chain of this plant.
Kawany Gabrieli Zanetti Fazoli, Laisa Marina Rosa Rey, Kariny Aparecida Jardim Rúbio, Mateus Augusto Garcia Souza, Halison Murilo da Silva Oliveira, Débora Cristina Ribeiro, Kelly Regina de Jesus Duarte Pereira, Denise Miyuki Kawamo, Thays Karollyni Amaral Gomes, Isadora Brito da Silva,et al.
MDPI AG
Bovine mastitis is an inflammation of the mammary gland in response to invasion by opportunistic agents. Due to the high economic importance of dairy production and the complexity related to animal health, the objective of this work was to identify and evaluate the antibacterial resistance profile of samples of mastitis milk, milking hand and milking equipment from small rural dairy farms belonging to the northwest region of the state of Paraná, Brazil. Five small, non-technical dairy farms in the municipalities of Boa Esperança, Juranda and Tapejara, all belonging to the northwest region of the state of Paraná, Brazil, were selected. The properties had Holstein and/or crossbred herds, carried out a bucket-by-foot milking system and all had the presence of animals with subclinical mastitis confirmed by the California Mastitis Test. Samples of sterile swabs from the milking insufflators, the milking hand and milk samples were collected—and later, isolation tests and phenotypic characterization of the samples, sensitivity tests to antimicrobials and phenotypic tests for the detection of beta-producing strains were performed with extended-spectrum beta-lactamase (ESBL), molecular identification of Staphylococcus aureus isolates and mecA gene research. Of the 199 samples collected from the 15 selected properties in the municipalities of Boa Esperança, Tapejara and Juranda, 72 (36.20%) were classified as multiresistant. Isolated from milkers’ hands and milking machines, which phenotypically produce extended-spectrum beta-lactamase (ESBL), the presence of the mecA gene was also observed in 11 isolates of Staphylococcus spp. of milk samples, machines and milking hands. Mastitis can be spread to the herd through the milking process by the milkers’ instruments and hands, and adequate management measures can prevent its transmission and the conscious use of antibiotics decreases the prevalence of multidrug-resistant pathogens. In this work, different pathogenic bacteria were detected in mastitic milk, milking equipment and milking hand with a high percentage (36.20%) of isolates classified as multidrug resistant. In addition, the presence phenotypically (ESBL) and molecularly (mecA gene) of isolates carrying resistance genes was also verified. These results directly reflect on the health of the animals, the health of the workers and the health of the respective environment, which can enable the continuity of the propagation of the etiological agents involved in the mastitis infection. The awareness of producers and workers on these properties about the disease, transmission, sanitary aspects and adequate management and treatment are essential for improving milk production and production efficiency.
Juliana Scanavacca, , Wanessa de Campos Bortolucci, Ezilda Jacomassi, Irineia Paulina Baretta, Maria Graciela Iecher Faria, Carla Maria Mariano Fernandez, Vele Tesevic, Jasmina Glamoclija, Marina Sokovic,et al.
MS-Editions
Food spoilage is a widely neglected problem and the constant use of synthetic fungicides could develop resistant fungi. The objective of this study was to evaluate the chemical composition and antimicrobial activity of Tetradenia riparia leaf essential oil against foodborne disease microorganisms. Leaf essential oil was obtained by hydrodistillation and identified by gas chromatography coupled to mass spectrometry. The antimicrobial activity was studied by broth microdilution. The major compounds identified were oxygenated sesquiterpenes (43.6%): 14-hydroxy-9-epi-(E)-cariophylene (20.8%) and τ-cadinol (18.4%); followed by oxygenated diterpenes (24.6%): 6,7-dehydroroyleanone (12.6%) and 9β, 13β-epoxy-7-abiethene (10.6%); sesquiterpenic hydrocarbons (17.1%) and oxygenated monoterpenes (7.4%): fenchone (5.6%). The essential oil had broad antibacterial and antifungal activity, mainly against A. versicolor and P. ochrochloron with fungistatic and fungicidal activities and B. cereus, L. monocytogenes, and S. aureus with bacteriostatic and bactericidal activities. T. riparia leaf essential oil is a potential alternative to control microorganisms.
Letícia Ferarrese, Herika Line Marko De Oliveira, Gabriella Santana De Oliveira, Juliana Aparecida Mendonça, Wilsandrei Cella, Mário De Luca Neto, Rodrigo Sadao Inumaro, Larissa Rafaela de Paula Ferreira, Heris Lorenzi dos Santos Perfeito, Ezilda Jacomassi,et al.
EDUFU - Editora da Universidade Federal de Uberlandia
Tetradenia riparia (Hochst.) Codd (Lamiaceae) is a shrub, commonly known as ginger bush or false myrrh, and several studies have shown that T. riparia exhibits a variety of biological properties. This study aimed to determine the chemical composition of T. riparia essential oil and its fractions, investigate their anticholinesterase activity, and assess their larvicidal activity against the cattle tick Rhipicephalus microplus and the mosquito Aedes aegypti. Eleven essential oil fractions were obtained by fractionation and analyzed by gas chromatography/mass spectrometry. Larvicidal activity against R. microplus and third-instar A. aegypti was assessed using a larval packet test and a larval immersion test, respectively. Anticholinesterase activity was determined by a bioautographic method. Forty-nine compounds were identified in the essential oil, of which the major classes were oxygenated sesquiterpenes (45.95%) and sesquiterpene hydrocarbons (35.20%) and the major components were isospathulenol (17.40%), β-caryophyllene (15.61%), 14-hydroxy-9-epi-caryophyllene (10.07%), 14-hydroxy-α-muurolene (8.32%), and 9β,13β-epoxy-7-abietene (5.53%). Bioassays showed that T. riparia essential oil (LC50 = 1.56 µg/mL) and FR3 (LC50 = 0.30 µg/mL) were the most active against R. microplus and A. aegypti larvae, respectively. The essential oil and FR1, FR2, and FR3 exhibited acetylcholinesterase inhibitory activity. These results indicate that T. riparia essential oil and its fractions hold promise in the development of novel, environmentally safe agents for the control of R. microplus and A. aegypti larvae.
Pereira Ruiz Herrig Suelen, Evellyn Claudia, Wietzikoski Lovato, Silveira do Valle Juliana, Cristiani Gazim Zilda, Camila Frederico, Daniela Lopes Ana, Andrea Linde Giani, Barros Colauto Nelson, and Graciela Iecher Faria Maria
CRC Press
L.A. Branco, B.F.A. Laginestra, M.B. Marçal, Z.C. Gazim, I.L. Rahal, S.P.R. Herrig, M.G.I.F. Nunes, J.V.M. Simões, D.D. Gonçalves, and R. Piau Junior
FapUNIFESP (SciELO)
ABSTRACT The aim of this study was to evaluate the antibacterial and antioxidant activities of essential oil (EO) from fresh leaves of Psidium rufum. The EO was extracted by hydrodistillation and identified by gas chromatography coupled to mass spectrometry. The antibacterial activity was evaluated by determining the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Antioxidant activity was determined by β-carotene/linoleic acid co-oxidation system, 2,2-diphenyl-1-picrylhydrazyl radical scavenging and iron reduction methods. Hydrocarbon sesquiterpenes were the predominant class, indicating 1,8 cineole, α-longipinene as major. The EO was tested against the bacteria Staphylococcus aureus and Pseudomonas aeruginosa (MIC = 2,500 µg/mL and MBC = 20,000 µg/mL); Enterococcus faecalis (MIC = 2,500µg/mL and MBC > 20,000µg/mL) and Escherichia coli (MIC > 20,000µg/mL and MBC > 20,000µg/mL). The EO showed antioxidant potential due to β-carotene/linoleic acid co-oxidation system, with 76.63% of oxidation inhibition (1.0mg/mL) and due to the iron reduction power (5,38 μmol Fe 2+ /mg sample). The results are promising in recommending this species for the development of food, cosmetic and pharmaceutical products.
Zilda Cristiani Gazim, Evellyn Claudia Wietzikoski Lovato, Bárbara de Souza Arcanjo, Maria Graciela Iecher Faria Nunes, Suelen Pereira Ruiz Herrig, Ana Daniela Lopes, Carla Maria Mariano Fernandez, Giani Andrea Linde Colauto, Nelson Barros Colauto, and Juliana Silveira do Valle
CRC Press
Carla Maria Mariano Fernandez, , Sirlene Adriana Kleinubing, Thaysa Ksiaskiewcz Karam, Fabiana Brusco Lorenzetti, Carolina Trevisolli Palomo, Byanca Pereira Moreira de Oliveira, Odinei Hess Goncalves, Zilda Cristiani Gazim, Mariza Barion Romagnolo,et al.
MS-Editions
The present study thus aimed at the development and physicochemical characterization of solid lipid nanoparticles loaded with crude extract of Piper corcovadensisroots (SLN-CEPc) and chitosan-coated solid lipid nanoparticles loaded withcrude extract of P. corcovadensisroots (C-SLN-CEPc), as well as the determination of its antimycobacterial activity against Mycobacterium tuberculosisH37Rv, its cytotoxicity against the Vero cell line and evaluation in the hemolysis assay. Both formulations containing the encapsulated extract showed high encapsulation efficiency, formed by a monodispersed system with small and spherical particles, and there was no aggregation of particles. In the biological assays, SLN-CEPc and C-SLN-CEPc showed promising anti-M. tuberculosisactivity with a minimum inhibitory concentration (MIC) of 12.5 μg/mL, whereas the cytotoxic concentrations obtained at 50% (CC50) in Vero cells were 60.0 and 70.0 μg/mL, respectively. Therefore, nanoencapsulation showed satisfactory results, justifying its usage in the development of new products.
Wilsandrei Cella, Isabelle Luiz Rahal, Gabriela Catuzo Canônico Silva, Ezilda Jacomassi, Ranulfo Piau Junior, José Eduardo Gonçalves, Daniela Dib Gonçalves, and Zilda Cristiani Gazim
FapUNIFESP (SciELO)
Abstract Around the world, the main problems of livestock are caused by ectoparasites, however, commercial acaracide are toxic to the environment and detrimental to One Health. Therefore, research has increasingly focused on development of natural products as alternatives for tick control. The purpose of this study was to evaluate the larvicidal effect on Rhipicephalus (Boophilus) microplus, through use of essential oils (EOs) extracted from the leaves, flower buds and stems of Tetradenia riparia. The chemical composition of these EOs was determined through gas chromatography coupled to mass spectrometry (GC-MS). They were tested on larvae at concentrations of 100.000 to 40 µg/mL, using the larval packet test and under semi-natural conditions. The main class of compounds in the chemical composition was sesquiterpenes (both oxygenates and hydrocarbons), whereas the predominant compounds in the leaves, flower buds and stems were 14-hydroxy-9-epi-caryophyllene, T-cadinol and 6-7-dehydroroyleanone, respectively. The leaves proved to be the most effective, with highest larvicidal activity (LC99.9 = 83.53 µg/mL). When tested under semi-natural conditions, the oils obtained efficiency above 98% in all compound tests. The results indicated that these EOs were effective against R. (B.) microplus larvae in vitro and ex-situ, proving that this plant has bioactive molecules with significant larvicidal activity.
Diego Baraldi Dedino, Julliane Destro de Lima, Wanessa de C. Bortolucci, Wesley R. Rivadavea, Evellyn C. W. Lovato, Zilda C. Gazim, José E. Gonçalves, Daisy L. R. Monzon, and Glacy J. da Silva
Springer Science and Business Media LLC
Zilda Cristiani Gazim, Juliana Silveira Valle, Isabela Carvalho dos Santos, Isabelle Luiz Rahal, Gabriela Catuzo Canonico Silva, Ana Daniela Lopes, Suelen Pereira Ruiz, Maria Graciela Iecher Faria, Ranulfo Piau Junior, and Daniela Dib Gonçalves
Frontiers Media SA
Baccharis dracunculifolia DC (Lamiaceae) (Asteraceae) is found in South America, mainly in Argentina, Brazil, Bolivia, Paraguay and Uruguay. Folk medicine is used as a sedative, hypotensive, bronchodilator, cardiovascular disorders, anti-flu, and also in skin wounds. Considered the main source of green propolis, which increases the pharmacological interest in this species. It is also known as a “benefactor” plant facilitating the development of other plant species around it, being indicated for the recovery of degraded areas. This species has been studied for decades in order to isolate and identify the active principles present in the aerial parts (leaves and flowers) and roots. The present study consists of a review of the scientific literature addressing the ethnobotanical, ethnomedicinal, phytochemical, pharmacological and potential cytotoxic effects of the B. dracunculifolia species. In this survey, we sought to investigate issues related to the botanical and geographic description of the species, the ethnobotanical uses, as well as the phytochemical studies of the essential oil, extracts and green propolis obtained from the aerial parts and roots of B. dracunculifolia. Using high precision analytical tools, numerous compounds have already been isolated and identified from leaves and flowers such as the flavonoids: naringenin, acacetin, dihydrokaempferol, isosakuranetin and kaempferide; phenolic acids: p-coumaric, dihydrocoumaric, ferulic (E)-cinnamic, hydroxycinnamic, gallic, caffeic, and several caffeoylquinic acids derivatives; phenolic acids prenylated: artepillin C, baccharin, drupanin; the glycosides dracuculifosides and the pentacyclic triterpenoids: Baccharis oxide and friedelanol. The predominant class in the essential oil of leaves and flowers are terpenoids comprising oxygenated monoterpenes and sesquiterpenes, highlighting the compounds nerolidol, spathulenol, germacrene D and bicyclogermacrene. These compounds give the species high antimicrobial, antioxidant, antitumor, analgesic, immunomodulatory and antiparasitic potential, making this species a promising herbal medicine. In vitro toxicity assays with B. dracunculifolia extract showed low or no cytotoxicity. However, in vivo analyses with high doses of the aqueous extract resulted in genotoxic effects, which leads us to conclude that the toxicity of this plant is dose-dependent.
Amanda Nascimento Vasques de Souza, Maria Graciela Iecher Faria, Cristiane Edna da Rocha, Gisele Strieder Philippsen, Gabriela Catuzo Canonico Silva, Gustavo Ratti da Silva, Rodrigo Sadao Inumaro, José Eduardo Gonçalves, Zilda Cristiani Gazim, Samantha Wietzikoski,et al.
Elsevier BV
Sujogya Kumar Panda, Luc Van Puyvelde, Marie Jeanne Mukazayire, and Zilda Cristiani Gazim
Frontiers Media SA
Centre of Environment Climate Change and Public Health, RUSA, Utkal University, Bhubaneswar, India, Department of Biology, Animal Physiology and Neurobiology Section, KU Leuven, Leuven, Belgium, Department of Pharmacy, School of Pharmacy and Medicine, College of Medicine and Health Sciences, University of Rwanda, Huye, Rwanda, Graduate Program in Animal Science and Biotechnology Applied to Agriculture, Chemistry Laboratory of Natural Products, Paranaense University, Umuarama, Brazil
Sujogya Kumar Panda, Zilda Cristiani Gazim, Shasank S. Swain, Marisa Cassia Vieira de Araujo Bento, Jéssica da Silva Sena, Marie Jeanne Mukazayire, Luc Van Puyvelde, and Walter Luyten
Frontiers Media SA
Tetradenia ripariaHochsteter codd. (Lamiaceae) in its native African continent, is considered one of the most popular aromatic medicinal plants. In folk medicine it may be used as an infusion to treat respiratory problems, cough, headache, stomach pain, diarrhea, fever, malaria, and dengue; and in the form of compresses it is applied for the relief of headaches and toothaches. The speciesT. ripariahas been researched for decades to isolate and identify chemical constituents present in extracts or essential oil obtained from the leaves, floral buds, or stems of this plant. The present study reviews the scientific literature on ethnomedicinal, phytochemical, and pharmacological aspects ofT. riparia. We discuss issues related to the botanical and geographical description of the species, ethnobotanical uses, phytochemical studies on its essential oil and extracts, and biological activities ofT. riparia.Several compounds have already been isolated from leaves, such as ibozol, 7α-hydroxyroileanone, 1′,2′-dideacetylboronolide, 8(14),15-sandaracopimaradiene-7α,18-diol; 5,6-dihydro-α-pyrone and α-pyrone. Terpenes predominated in the essential oil, comprising monoterpenes, sesquiterpenes, diterpenes, hydrocarbons, and oxygenates. Most phytocompounds were isolated from the leaves and flower buds, namely fenchone, 14-hydroxy-9-epi (E)-caryophyllene, 9β, 13β-epoxy-7-abietene, and 6,7-dehydroroileanone. These compounds provide the species a high pharmacological potential, with antimicrobial, antioxidant, antitumor, analgesic, anti-leishmania, anti-tuberculosis, and anti-parasitic activities. Therefore, this species is a promising herbal medicine.
Débora C Montaholi, Tamires L Valverde, Eloísa G Sampiron, Wanessa C Bortoluci, Zilda C Gazim, Katiany R Caleffi-Ferracioli, Regiane BL Scodro, Vera LD Siqueira, and Rosilene F Cardoso
Future Medicine Ltd
Background: There is critical need for new therapeutic options for treatment of diseases caused by mycobacteria. Materials & methods: Gallesia integrifolia essential oils (EOs) and crude extracts (CEs) were tested for their anti- Mycobacterium tuberculosis and anti-nontuberculous mycobacteria activity. Results: Minimum inhibitory concentration (MIC) of EOs ranged from 15.63 to 62.5 μg/ml against M. tuberculosis and 62.5 to >250 μg/ml against nontuberculous mycobacteria. CEs showed low activity. All EO tested demonstrated synergism with antituberculosis drugs. The cytotoxicity of EOs and CEs, in different cell lines, showed selectivity index from 2.2 to 9.8 and >0.056 to 2.0, respectively. Conclusion: G. integrifolia EOs are a candidate for the development of new therapeutic options in the treatment of tuberculosis and other mycobacterial diseases.
Sara Jane Marsola, Lais Freitas Jorge, Adriano Borges Meniqueti, Miria Benetati Delgado Bertéli, Thaís Emanuelle Feijó de Lima, José Luiz Bezerra, Ana Daniela Lopes, Zilda Cristiani Gazim, Juliana Silveira do Valle, Nelson Barros Colauto,et al.
Springer Science and Business Media LLC
Cristine Bonacina, Rayane Monique Sete da Cruz, Andressa Bezerra Nascimento, Letícia Neris Barbosa, José Eduardo Gonçalves, Zilda Cristiani Gazim, Hélida Mara Magalhães, and Silvia Graciele Hülse de Souza
Elsevier BV
Andreia Fuentes Santos, , Marilia Moraes Queiroz Souza, Karoline Bach Pauli, Gustavo Ratti da Silva, Midia Wolff Marques, Pablo Alvarez Auth, Wanessa de Campos Bortolucci, Zilda Cristiani Gazim, Giani Andrea Linde,et al.
MS-Editions
Bacopa monnieri(L.) Wettst. (Plantaginaceae), also known as Brahmi, has been used to improve cognitive processes and intellectual functions that are related to the preservation of memory. The objective of this research is to review the ethnobotanical applications, phytochemical composition, toxicity and activity of B. monnieriin the central nervous system. It reviewed articles on B. monnieriusing Google Scholar, SciELO, Science Direct, Lilacs, Medline, and PubMed. Saponins are the main compounds in extracts of B. monnieri. Pharmacological studies showed that B. monnieriimproves learning and memory and presents biological effects against Alzheimer’s disease, Parkinson’s disease, epilepsy, and schizophrenia. No preclinical acute toxicity was reported. However, gastrointestinal side effects were reported in some healthy elderly individuals. Most studies with B. monnierihave been preclinical evaluations of cellular mechanisms in the central nervous system and further translational clinical research needs to be performed to evaluate the safety and efficacy of the plant.
Bruna de Fatima Antunes Laginestra, Thaís Camaso de Sá, Jessé Lahos Borges, Matheus Basilio Marçal, João Vitor Morais Simões, Selma Alves Rodrigues, Zilda Cristiani Gazim, and Ranulfo Piau Júnior
Georg Thieme Verlag KG
Abstract Background Bovine mastitis is characterized by an inflammatory process in the mammary gland and represents one of the main diseases affecting a dairy herd. Management of mastitis is most commonly via antibiotics, but the rising incidence of multiple antibiotic resistance (MAR) means that additional options are needed. Homeopathic products can be administered in dairy farming for a range of clinical reasons and may be preferential due to the absence of residues. Objectives The aim of this study was to assess the potential of a novel homeopathic complex medicine in managing bovine mastitis. Methods Twenty-four lactating Holstein cows with mastitis were divided into two groups: the homeopathic complex group received a homeopathic complex daily for 60 days at a dose of 20 g/d; the placebo group received the calcium carbonate vehicle without homeopathic medicines at the same dose and repetition. The main outcome measure was somatic cell count (SCC; cells/mL), with additional outcome measures including milk production (kg/d), milk constituents (percentage of protein, fat, lactose and total milk solids), and serum levels of cortisol, glucose, ammonia and lactic acid. All outcomes were measured at the beginning of the study and after 30 and 60 days. Milk samples were also collected from all animals at the beginning of the study, confirming a high (>0.2) MAR index for isolated bacterial cultures. Results Assessment of SCC showed a statistically significant difference favoring the homeopathic complex versus placebo group at day 60. A reduction in serum cortisol levels and an increase in fat, lactose and total milk solids in animals treated with the homeopathic complex at day 60 were also seen. Other outcome measures did not show statistically significant inter-group differences. Conclusion The results of this non-randomized, open-label, placebo-controlled trial suggest the potential for a novel homeopathic complex medicine in management of multiple antibiotic-resistant bovine mastitis, thus offering dairy farmers an additional option to antibiotics and making dairy products safer for consumer health and milk production more sustainable.