Zilda Cristiani Gazim

Zilda Cristiani Gazim Researcher at CNPq level 1D since 2022. She coordinated the lato sensu postgraduate course - Specialization in Handling Pharmaceuticals and Cosmetics, class I (2005-2006); class II (2006-2007) and class III (2007-2008). Since February 2000, he has been part of the faculty of the Universidade Paranaense (UNIPAR) teaching Pharmacotechnics, Cosmetology, Homeopathic Pharmacotechnics, Quality Control, Phytocosmetology and Aromatherapy , Biocomposites and Bioassays. In 2013 she joined the faculty of the Postgraduate Program in Biotechnology Applied to Agriculture - master's and doctoral level at Universidade Paranaense-UNIPAR. In 2021 she joined the faculty of the Graduate Program in Animal Science with an emphasis on bioactive products. Expertise in Chemistry of Natural Products, working in the areas: Essential Oils, Volatile Substances of Plants, chromatographic isolation of biomolecules, chemical identification of natural products, Gas Chromatography and Mass Spectro

EDUCATION

PhD (UEM, Maringá-PR, 2010) and Master's (UEM, Maringá-PR, 2005) in Pharmaceutical Sciences: Area of Concentration: Natural biologically active products from the State University of Maringá ( EMU). Degree in Pharmacy from the Federal University of Rio de Janeiro (UFRJ) Rio de Janeiro-RJ (1988) with qualifications in: Industrial Pharmacist from the Federal University of Rio de Janeiro (UFRJ) Rio de Janeiro-RJ (1989) and Biochemical Pharmacist Analysis of Food by the Federal University of Rio de Janeiro (UFRJ) Rio de Janeiro-RJ (1991). Specialization in Homeopathy by the Hahnemannian Institute of Brazil (IHB) Rio de Janeiro-RJ (1993); Specialization in Pharmacology at Universidade Paranaense (UNIPAR) Umuarama-PR (1999).

RESEARCH, TEACHING, or OTHER INTERESTS

Pharmaceutical Science, Plant Science, Pharmacology, Toxicology and Pharmaceutics, General Pharmacology, Toxicology and Pharmaceutics

Scopus Publications

Effects of Baccharis dracunculifolia DC on an Innovative Animal Model of Cardiometabolic Syndrome
Gustavo Ratti da Silva, Arianne Jung Kluck, Edilson Rodrigues Albuquerque, Lucas Pires Guarnier, Fernanda de Abreu Braga, Ester Pelegrini Silva, Karina Sposito Negrini, Juliana Aparecida Mendonça, Zilda Cristiani Gazim, Arquimedes Gasparotto Junior,et al.
MDPI AG
Background/Objective: Cardiometabolic syndrome (CMS) is a complex clinical condition that encompasses metabolic dysregulation, cardiovascular disease, and diabetes risk factors. Worldwide, CMS is underdiagnosed, and its occurrence significantly increases cardiovascular morbimortality. Despite available pharmacological treatments, the approach is fragmented, and the associated clinical conditions are treated independently. This approach may be partially due to limited preclinical models to mimic the clinical conditions of CMS. Therefore, our study aims to present an innovative animal model of cardiometabolic syndrome and evaluate the effects of Baccharis dracunculifolia on the set of clinical alterations associated with the condition. Methods: Female Wistar rats were induced to develop diabetes, fed a cholesterol-enriched diet, and exposed to the smoke of 9 cigarettes/day for 6 weeks. During the last 2 weeks, the rats were treated with vehicle, B. dracunculifolia (30, 100, and 300 mg/kg), or a combination of simvastatin and insulin. At the end of the treatment, plasma lipid levels were measured, and the liver was analyzed histologically for hepatic lipid quantification and oxidative stress assessment. Results: Phytochemical analysis revealed seven phenolic acids and six flavonoids in the extract. B. dracunculifolia showed significant hepatoprotective effects, reducing AST and ALT levels and lowering both plasma and hepatic lipid levels. The extract also reversed hepatic steatosis and demonstrated antioxidant properties. Conclusions: These findings suggest that B. dracunculifolia may be a therapeutic option for the metabolic dysregulation present in CMS.


Antioxidant Activity, Antiproliferative Activity, Antiviral Activity, NO Production Inhibition, and Chemical Composition of Essential Oils and Crude Extracts of Leaves, Flower Buds, and Stems of Tetradenia riparia
Jéssica da Silva Sena, Selma Alves Rodrigues, Karina Sakumoto, Rodrigo Sadao Inumaro, Pamela González-Maldonado, Emilio Mendez-Scolari, Ranulfo Piau, Daniela Dib Gonçalves, Filipa Mandim, Josiana Vaz,et al.
MDPI AG
The chemical composition of extracts (CEs) and essential oils (EOs) from Tetradenia riparia leaves, flower buds, and stems was analyzed. Antiproliferative activity against tumor cell lines, NO production inhibition, and antioxidant and antiviral activities were assessed. The CEs contained flavonoids, phenolic acids, coumarins, and saturated fatty acids. The EOs included monoterpenes, oxygenated sesquiterpenes, and diterpenes. NO production inhibition ranged from 76 to 247 µg mL−1, and antiproliferative activity exhibited GI50 between 20 and >204 µg mL−1, with low cytotoxicity (SI: 1.08 to 4.75). Reactive oxygen species inhibition ranged from 45 to 82%. Antioxidant activity varied when determined by the 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay (IC50: 0.51 to 8.47 mg mL−1) and ferric reducing antioxidant power (0.35 to 0.81 µM ferrous sulfate per mg). The reduction in β-carotene–linoleic acid co-oxidation varied between 76.13 and 102.25%. The total phenolic content of CEs and EOs was 10.70 to 111.68 µg gallic acid mg−1. Antiviral activity against herpes simplex virus type 1 (HSV-1) showed an EC50 between 9.64 and 24.55 µg mL−1 and an SI between 8.67 and 15.04. Leaf EOs exhibited an EC50 of 9.64 µg mL−1 and an SI of 15.04. Our study unveils the diverse chemical composition and multifaceted pharmacological properties of T. riparia, demonstrating its potential as a valuable source of bioactive compounds for therapeutic applications.


Bioactives of the essential oil from the leaves of Eugenia pyriformis Cambess (Myrtaceae) on the effects of tobacco
Jaqueline Pavelegini de Medeiros, Selma Alves Rodrigues, Karina Sakumoto, Suelen Pereira Ruiz, Maria Graciela Iecher Faria, José Eduardo Gonçalves, Ranulfo Piau Junior, Jasmina Glamočlija, Marina Soković, Daniela Dib Gonçalves,et al.
Frontiers Media SA
IntroductionLung cancer is the most commonly diagnosed and the main cause of cancer death, usually related to cigarette smoking. Furthermore, the microbiota of people exposed to cigarette smoke can be modified, making it difficult to eliminate opportunistic microorganisms. The leaves of Eugenia pyriformis are a by-product of fruit production and, to date, there have been no studies addressing the antiproliferative, anti-inflammatory, and antimicrobial activities.ObjectiveInvestigate the antimicrobial, Nitric Oxide (NO)-production inhibition, and antiproliferative activities of the essential oil from E. pyriformis leaves and its possible effect on the treatment and prevention of damage caused by tobacco.MethodsThe essential oil (EO) was obtained by hydrodistillation (3 h). Its chemical composition was investigated by GC-MS. It was proposed to investigate antiproliferative activity against human tumor cell lines, namely, breast adenocarcinoma (MCF-7), lung (NCI-H460), cervical (HeLa), and hepatocellular (HepG2) carcinomas. A non-tumor primary culture from pig liver (PLP2) was also tested. The EO capacity to inhibit nitric oxide (NO) production was evaluated by a lipopolysaccharide stimulated murine macrophage cell line. Antibacterial and antifungal activities against opportunistic pathogens were investigated against seven strains of bacteria and eight fungi.ResultsThe results indicated the presence of 23 compounds in the essential oil, the majority were spathulenol (45.63%) and β-caryophyllene oxide (12.72%). Leaf EO provided 50% inhibition of nitric oxide production at a concentration of 92.04 µg mL−1. The EO also demonstrated antiproliferative activity against all human tumor cell lines studied, with GI50 values comprised between 270.86 and 337.25 µg mL−1. The essential oil showed antimicrobial potential against the bacteria Listeria monocytogenes (Murray et al.) Pirie (NCTC 7973) and Salmonella Typhimurium ATCC 13311 (MIC 1870 µg mL−1) and fungi Aspergillus versicolor ATCC 11730, Aspergillus ochraceus ATCC 12066, Penicillium ochrochloron ATCC 90288, Penicillium verrucosum var. cyclopium (Westling) Samson, Stolk & Hadlok (food isolate) (MIC 1870 µg mL−1) and Trichoderma viride Pers. IAM 5061 (1,400 µg mL–1).ConclusionThe demonstrated anti-inflammatory, antiproliferative, and antimicrobial activities in the leaves of E. pyriformis can add value to the production chain of this plant, being a possible option for preventing and combating cancer, including lung cancer.


Pachira aquatica: biological activity and chemical composition of leaves, flowers, and seeds
Isabelle Luiz Rahal, Angelica Barbosa Dias, Gabriela Catuzo Canonico Silva, Marisa Cássia Vieira de Araújo Bento, Rhaira Fernanda Ayob Casalvara, José Eduardo Gonçalves, Nelson Barros Colauto, Giani Andrea Linde, Filipa Mandim, Josiana Vaz,et al.
Informa UK Limited
The chemical composition of Pachira aquatica crude extracts flowers, leaves, and seeds was obtained by UHPLC-ESI/qTOF and GC/MS. The antiproliferative activity was evaluated against the human tumour cell lines AGS (gastric), CaCo-2 (colorectal), MCF-7 (breast), and NCI-H460 (lung). The anti-inflammatory and cellular antioxidant activities were also studied. Flavonoids, phenolic acids, coumarins, and saturated fatty acids were identified in the samples. The concentration of extracts responsible for inhibiting 50% of nitric oxide production ranged from (149 to > 400 µg mL-1). Antiproliferative activity against the tumour cell lines was: AGS (GI50 175 to > 400 µg mL-1), Caco-2 (GI50 215 to > 400 µg mL-1), MCF7 (GI50 232 to > 400 µg mL-1) and NCI-H460 (GI50 208 to > 400 µg mL-1). Cellular antioxidant activity remained between 73% to > 2000%. The selectivity index (SI) ranged from 1.00 to 2.78, indicating low antiproliferative activity.


Essential Oil from the Leaves, Fruits and Twigs of Schinus terebinthifolius: Chemical Composition, Antioxidant and Antibacterial Potential
Kátia C. Oliveira, Lidaiane M. S. S. Franciscato, Suelen S. Mendes, Francielly M. A. Barizon, Daniela D. Gonçalves, Lidiane N. Barbosa, Maria G. I. Faria, Juliana S. Valle, Rhaira F. A. Casalvara, José E. Gonçalves,et al.
MDPI AG
Schinus terebinthifolius Raddi, popularly known as “Pink pepper”, is a plant native to Brazil. The objective of this work was to analyze the chemical composition and the antioxidant and antibacterial potential of essential oils (EOs) from the leaves, fruits and twigs of S. terebinthifolius, aiming for their application in food safety. EOs were obtained by hydrodistillation and the chemical composition was determined by gas chromatography coupled to mass spectrometry. Phenolic compounds were quantified and antioxidant activity was evaluated using three different methods. The antibacterial activity was determined by the broth microdilution method against foodborne bacteria. In the chemical analysis, 22 compounds were identified in the leaves, 13 compounds in the fruits and 37 compounds in the twigs, revealing the presence of the main compounds germacrene D (12.04%, 15.78%, 20,41%), caryophyllene (15.97%, 3.12%, 11.73%), α-pinene (11.6%, 17.16%, 2.99%), β-pinene (5.68%, 43.34%, 5.60%) and γ-gurjunene (16,85%, 3,15%) respectively. EOs showed better antioxidant potential using the β-carotene/linoleic acid method with 40.74, 61.52 and 63.65% oxidation inhibition for leaves, fruits and twigs, respectively. The EO from the leaves showed greater antibacterial potential against Escherichia coli and Staphylococcus aureus with a minimum inhibitory concentration (MIC) of 0.62 mg mL−1, a value lower than the MIC of sodium nitrite (5.00 mg mL−1), the antimicrobial standard synthetic. The activities of pink pepper EOs suggest their potential as a biopreservative in foods.


Methyl jasmonate and copper activate volatiles and antioxidant mechanisms in 'Grecco a Palla' basil produced in vitro
Felipe Górski, Geysiane Moreira Gerotti, José Eduardo Gonçalves, Zilda Cristiani Gazim, and Hélida Mara Magalhães
Springer Science and Business Media LLC



Relations Between Guardians and Domestic Animals During the Sars-Cov-2 Pandemic in Brazil
Ailton da Cruz Melo, Isabela Carvalho dos Santos, Adriane Cordeiro Trevisani, Kariny Aparecida Jardim Rúbio, Ana Luisa Cano, Eloiza Teles Caldart, Juliana Aparecida Mendonça, Renata Olivotto Agostinis, Ranulfo Piau Junior, Zilda Cristiani Gazim,et al.
Cambridge University Press (CUP)
Abstract Objective: The aim of this study was to evaluate how relationships between guardians and domestic animals were established and their possible effects during the COVID-19 pandemic period in Brazil. Methods: Data were collected by completing an online questionnaire. Throughout the national territory, 2002 people completed the questionnaire, while respecting social distancing from January 20, 2021, to March 20, 2021. The inclusion and exclusion criteria were ‘to own pets’ or ‘those who had them during the period of quarantine’ in Brazil. The data obtained were treated qualitatively through discourse analysis and content analysis, while the quantitative data were tabulated by the questionnaire application platform itself. Results: The results showed that 97% of the participants were affected by the coronavirus pandemic in Brazil and that of these, 95.5% stated that their pet was important to overcome and bear bad feelings during the period of social isolation. It was possible to observe through the participants’ reports through the online questionnaire that the relationships with their pets were deepened during the pandemic period, and that these had an important role in overcoming bad emotions caused by social distancing. Conclusion: It was concluded that due to these relationships, behaviors such as anxiety and sadness were reduced and the pets themselves also showed an increase in affective behaviors in relation to their guardians.


Cellular Antioxidant, Anti-Inflammatory, and Antiproliferative Activities from the Flowers, Leaves and Fruits of Gallesia integrifolia Spreng Harms
Gabriela Catuzo Canônico Silva, Mariane de Almeida Machado, Karina Sakumoto, Rodrigo Sadao Inumaro, José Eduardo Gonçalves, Filipa Mandim, Josiana Vaz, Juliana Silveira do Valle, Maria Graciela Iecher Faria, Suelen Pereira Ruiz,et al.
MDPI AG
Gallesia integrifolia, a notable species in the Atlantic Forest, has been traditionally employed in folk medicine for treating rheumatism, asthma, and worms. This study investigated the cellular antioxidant, antiproliferative, and anti-inflammatory activities of the essential oils (EOs) and crude extracts (CEs) from G. integrifolia flowers, fruits, and leaves. The chemical identification of EOs was performed by GC–MS and CEs by UHPLC–MS. Cellular antioxidant and anti-inflammatory activities were assessed through mouse macrophage cell culture. In addition, the antiproliferative potential was evaluated in gastric, colorectal, breast, and lung tumor cell lines and non-tumor VERO cells. EOs predominantly contained organosulfur compounds in flowers (96.29%), fruits (94.94%), and leaves (90.72%). We found the main compound is 2,2′-Disulfanediyldiethanethiol in the EOs of flowers (47.00%), leaves (41.82%), and fruits (44.39%). Phenolic compounds were identified in CEs. The EOs and CEs demonstrated potential against the tumor cell lines tested (GI50 between 51 and 230 µg/mL). The selectivity index values were greater than 1.0 (1.01 to 3.37), suggesting a relative safety profile. Moreover, the anti-inflammatory activity IC50 ranged from 36.00 to 268 µg/mL, and the cellular oxidation inhibition ranged from 69% to 82%. The results suggest that oils and extracts derived from G. integrifolia have potential for use in various industrial sectors.


Impact of kinetin on essential oil content in Acmella oleracea
Nastassja Kimberlly Lima, Eloisa Schneider da Silva, Rayane Monique Sete da Cruz, Julliane Destro de Lima, Cristian Coelho da Silva, Mayara dos Santos Queiroz, Zilda Cristiani Gazim, José Eduardo Gonçalves, Daisy Leticia Ramirez Monzon, Renan Rizzato Espessato,et al.
Springer Science and Business Media LLC



Valorization of Campomanesia xanthocarpa leaves: Chemical composition and antioxidant activity of crude extract and fractions
Rosangela Rumi Sugauara, , Wanessa de Campos Bortolucci, Carla Maria Mariano Fernandez, Marcia Regina Pereira Cabral, Regina Aparecida Correia Goncalves, Maria Helena Sarragiotto, Jose Eduardo Goncalves, Nelson Barros Colauto, Giani Andrea Linde,et al.
MS-Editions
Campomanesia xanthocarpaleaves are a byproduct of fruit production without studies on antioxidant activity. Thus, this study aimed to identify the antioxidant compounds of C. xanthocarpaleaves by ultra-high performance liquid chromatography coupled with electrospray ionization-quadrupole-time of flight-mass spectrometry (UHPLC-ESI/qTOF) and by different in vitroantioxidant methods. The crude extract of C. xanthocarpaleaves had a yield of 15.2% and only five out of 37 fractions of the crude extract had antioxidant activity. The crude extract presented greater antioxidant activity than the isolated fractions. The identified antioxidant compounds were phenolic acids (gallic acid and chlorogenic acid), flavonoids (quercetin and naringenin 7,4’-dimethoxy) and an organic acid (quinic acid). Leaves of C. xanthocarpahave high concentration of antioxidant compounds and it is a promising plant for the development of applications in the food, cosmetic, and pharmaceutical fields. The extraction of antioxidant compounds can add value to the productive chain of this plant.


Resistance Profile of Bovine Mastitis Isolates, Presence of the mecA Gene and Identification of ESBL Producing Strains from Small Rural Dairy Properties
Kawany Gabrieli Zanetti Fazoli, Laisa Marina Rosa Rey, Kariny Aparecida Jardim Rúbio, Mateus Augusto Garcia Souza, Halison Murilo da Silva Oliveira, Débora Cristina Ribeiro, Kelly Regina de Jesus Duarte Pereira, Denise Miyuki Kawamo, Thays Karollyni Amaral Gomes, Isadora Brito da Silva,et al.
MDPI AG
Bovine mastitis is an inflammation of the mammary gland in response to invasion by opportunistic agents. Due to the high economic importance of dairy production and the complexity related to animal health, the objective of this work was to identify and evaluate the antibacterial resistance profile of samples of mastitis milk, milking hand and milking equipment from small rural dairy farms belonging to the northwest region of the state of Paraná, Brazil. Five small, non-technical dairy farms in the municipalities of Boa Esperança, Juranda and Tapejara, all belonging to the northwest region of the state of Paraná, Brazil, were selected. The properties had Holstein and/or crossbred herds, carried out a bucket-by-foot milking system and all had the presence of animals with subclinical mastitis confirmed by the California Mastitis Test. Samples of sterile swabs from the milking insufflators, the milking hand and milk samples were collected—and later, isolation tests and phenotypic characterization of the samples, sensitivity tests to antimicrobials and phenotypic tests for the detection of beta-producing strains were performed with extended-spectrum beta-lactamase (ESBL), molecular identification of Staphylococcus aureus isolates and mecA gene research. Of the 199 samples collected from the 15 selected properties in the municipalities of Boa Esperança, Tapejara and Juranda, 72 (36.20%) were classified as multiresistant. Isolated from milkers’ hands and milking machines, which phenotypically produce extended-spectrum beta-lactamase (ESBL), the presence of the mecA gene was also observed in 11 isolates of Staphylococcus spp. of milk samples, machines and milking hands. Mastitis can be spread to the herd through the milking process by the milkers’ instruments and hands, and adequate management measures can prevent its transmission and the conscious use of antibiotics decreases the prevalence of multidrug-resistant pathogens. In this work, different pathogenic bacteria were detected in mastitic milk, milking equipment and milking hand with a high percentage (36.20%) of isolates classified as multidrug resistant. In addition, the presence phenotypically (ESBL) and molecularly (mecA gene) of isolates carrying resistance genes was also verified. These results directly reflect on the health of the animals, the health of the workers and the health of the respective environment, which can enable the continuity of the propagation of the etiological agents involved in the mastitis infection. The awareness of producers and workers on these properties about the disease, transmission, sanitary aspects and adequate management and treatment are essential for improving milk production and production efficiency.


Antimicrobial activity of Tetradenia riparia leaf essential oil
Juliana Scanavacca, , Wanessa de Campos Bortolucci, Ezilda Jacomassi, Irineia Paulina Baretta, Maria Graciela Iecher Faria, Carla Maria Mariano Fernandez, Vele Tesevic, Jasmina Glamoclija, Marina Sokovic,et al.
MS-Editions
Food spoilage is a widely neglected problem and the constant use of synthetic fungicides could develop resistant fungi. The objective of this study was to evaluate the chemical composition and antimicrobial activity of Tetradenia riparia leaf essential oil against foodborne disease microorganisms. Leaf essential oil was obtained by hydrodistillation and identified by gas chromatography coupled to mass spectrometry. The antimicrobial activity was studied by broth microdilution. The major compounds identified were oxygenated sesquiterpenes (43.6%): 14-hydroxy-9-epi-(E)-cariophylene (20.8%) and τ-cadinol (18.4%); followed by oxygenated diterpenes (24.6%): 6,7-dehydroroyleanone (12.6%) and 9β, 13β-epoxy-7-abiethene (10.6%); sesquiterpenic hydrocarbons (17.1%) and oxygenated monoterpenes (7.4%): fenchone (5.6%). The essential oil had broad antibacterial and antifungal activity, mainly against A. versicolor and P. ochrochloron with fungistatic and fungicidal activities and B. cereus, L. monocytogenes, and S. aureus with bacteriostatic and bactericidal activities. T. riparia leaf essential oil is a potential alternative to control microorganisms.


POTENTIAL OF Tetradenia riparia LEAF ESSENTIAL OIL AND ITS FRACTIONS IN CONTROLLING Aedes aegypti AND Rhipicephalus microplus LARVAE
Letícia Ferarrese, Herika Line Marko De Oliveira, Gabriella Santana De Oliveira, Juliana Aparecida Mendonça, Wilsandrei Cella, Mário De Luca Neto, Rodrigo Sadao Inumaro, Larissa Rafaela de Paula Ferreira, Heris Lorenzi dos Santos Perfeito, Ezilda Jacomassi,et al.
EDUFU - Editora da Universidade Federal de Uberlandia
Tetradenia riparia (Hochst.) Codd (Lamiaceae) is a shrub, commonly known as ginger bush or false myrrh, and several studies have shown that T. riparia exhibits a variety of biological properties. This study aimed to determine the chemical composition of T. riparia essential oil and its fractions, investigate their anticholinesterase activity, and assess their larvicidal activity against the cattle tick Rhipicephalus microplus and the mosquito Aedes aegypti. Eleven essential oil fractions were obtained by fractionation and analyzed by gas chromatography/mass spectrometry. Larvicidal activity against R. microplus and third-instar A. aegypti was assessed using a larval packet test and a larval immersion test, respectively. Anticholinesterase activity was determined by a bioautographic method. Forty-nine compounds were identified in the essential oil, of which the major classes were oxygenated sesquiterpenes (45.95%) and sesquiterpene hydrocarbons (35.20%) and the major components were isospathulenol (17.40%), β-caryophyllene (15.61%), 14-hydroxy-9-epi-caryophyllene (10.07%), 14-hydroxy-α-muurolene (8.32%), and 9β,13β-epoxy-7-abietene (5.53%). Bioassays showed that T. riparia essential oil (LC50 = 1.56 µg/mL) and FR3 (LC50 = 0.30 µg/mL) were the most active against R. microplus and A. aegypti larvae, respectively. The essential oil and FR1, FR2, and FR3 exhibited acetylcholinesterase inhibitory activity. These results indicate that T. riparia essential oil and its fractions hold promise in the development of novel, environmentally safe agents for the control of R. microplus and A. aegypti larvae.


Chemical Composition and Antimicrobial Activity of Plant Essential Oils from the Phytolaccaceae and Petiveriaceae Families
Pereira Ruiz Herrig Suelen, Evellyn Claudia, Wietzikoski Lovato, Silveira do Valle Juliana, Cristiani Gazim Zilda, Camila Frederico, Daniela Lopes Ana, Andrea Linde Giani, Barros Colauto Nelson, and Graciela Iecher Faria Maria
CRC Press



Chemical composition and antioxidant activity of crude extracts from Pachira aquatica leaves, flowers and seeds




Evaluation of antibacterial and antioxidant activity of purple araçá essential oil (Psidium rufum, Myrtaceae)
L.A. Branco, B.F.A. Laginestra, M.B. Marçal, Z.C. Gazim, I.L. Rahal, S.P.R. Herrig, M.G.I.F. Nunes, J.V.M. Simões, D.D. Gonçalves, and R. Piau Junior
FapUNIFESP (SciELO)
ABSTRACT The aim of this study was to evaluate the antibacterial and antioxidant activities of essential oil (EO) from fresh leaves of Psidium rufum. The EO was extracted by hydrodistillation and identified by gas chromatography coupled to mass spectrometry. The antibacterial activity was evaluated by determining the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Antioxidant activity was determined by β-carotene/linoleic acid co-oxidation system, 2,2-diphenyl-1-picrylhydrazyl radical scavenging and iron reduction methods. Hydrocarbon sesquiterpenes were the predominant class, indicating 1,8 cineole, α-longipinene as major. The EO was tested against the bacteria Staphylococcus aureus and Pseudomonas aeruginosa (MIC = 2,500 µg/mL and MBC = 20,000 µg/mL); Enterococcus faecalis (MIC = 2,500µg/mL and MBC > 20,000µg/mL) and Escherichia coli (MIC > 20,000µg/mL and MBC > 20,000µg/mL). The EO showed antioxidant potential due to β-carotene/linoleic acid co-oxidation system, with 76.63% of oxidation inhibition (1.0mg/mL) and due to the iron reduction power (5,38 μmol Fe 2+ /mg sample). The results are promising in recommending this species for the development of food, cosmetic and pharmaceutical products.


Phytolaccaceae and Petiveriaceae Ethnobotany and Phytochemistry
Zilda Cristiani Gazim, Evellyn Claudia Wietzikoski Lovato, Bárbara de Souza Arcanjo, Maria Graciela Iecher Faria Nunes, Suelen Pereira Ruiz Herrig, Ana Daniela Lopes, Carla Maria Mariano Fernandez, Giani Andrea Linde Colauto, Nelson Barros Colauto, and Juliana Silveira do Valle
CRC Press



Development, characterization and anti-Mycobacterium tuberculosis activity of solid lipid nanoparticles for oral Piper corcovadensis roots extract delivery
Carla Maria Mariano Fernandez, , Sirlene Adriana Kleinubing, Thaysa Ksiaskiewcz Karam, Fabiana Brusco Lorenzetti, Carolina Trevisolli Palomo, Byanca Pereira Moreira de Oliveira, Odinei Hess Goncalves, Zilda Cristiani Gazim, Mariza Barion Romagnolo,et al.
MS-Editions
The present study thus aimed at the development and physicochemical characterization of solid lipid nanoparticles loaded with crude extract of Piper corcovadensisroots (SLN-CEPc) and chitosan-coated solid lipid nanoparticles loaded withcrude extract of P. corcovadensisroots (C-SLN-CEPc), as well as the determination of its antimycobacterial activity against Mycobacterium tuberculosisH37Rv, its cytotoxicity against the Vero cell line and evaluation in the hemolysis assay. Both formulations containing the encapsulated extract showed high encapsulation efficiency, formed by a monodispersed system with small and spherical particles, and there was no aggregation of particles. In the biological assays, SLN-CEPc and C-SLN-CEPc showed promising anti-M. tuberculosisactivity with a minimum inhibitory concentration (MIC) of 12.5 μg/mL, whereas the cytotoxic concentrations obtained at 50% (CC50) in Vero cells were 60.0 and 70.0 μg/mL, respectively. Therefore, nanoencapsulation showed satisfactory results, justifying its usage in the development of new products.


Activity of essential oils from leaves, flower buds and stems of Tetradenia riparia on Rhipicephalus (Boophilus) microplus larvae
Wilsandrei Cella, Isabelle Luiz Rahal, Gabriela Catuzo Canônico Silva, Ezilda Jacomassi, Ranulfo Piau Junior, José Eduardo Gonçalves, Daniela Dib Gonçalves, and Zilda Cristiani Gazim
FapUNIFESP (SciELO)
Abstract Around the world, the main problems of livestock are caused by ectoparasites, however, commercial acaracide are toxic to the environment and detrimental to One Health. Therefore, research has increasingly focused on development of natural products as alternatives for tick control. The purpose of this study was to evaluate the larvicidal effect on Rhipicephalus (Boophilus) microplus, through use of essential oils (EOs) extracted from the leaves, flower buds and stems of Tetradenia riparia. The chemical composition of these EOs was determined through gas chromatography coupled to mass spectrometry (GC-MS). They were tested on larvae at concentrations of 100.000 to 40 µg/mL, using the larval packet test and under semi-natural conditions. The main class of compounds in the chemical composition was sesquiterpenes (both oxygenates and hydrocarbons), whereas the predominant compounds in the leaves, flower buds and stems were 14-hydroxy-9-epi-caryophyllene, T-cadinol and 6-7-dehydroroyleanone, respectively. The leaves proved to be the most effective, with highest larvicidal activity (LC99.9 = 83.53 µg/mL). When tested under semi-natural conditions, the oils obtained efficiency above 98% in all compound tests. The results indicated that these EOs were effective against R. (B.) microplus larvae in vitro and ex-situ, proving that this plant has bioactive molecules with significant larvicidal activity.


Red LED light and different cultivation methods changed the essential oil composition of Acmella oleracea
Diego Baraldi Dedino, Julliane Destro de Lima, Wanessa de C. Bortolucci, Wesley R. Rivadavea, Evellyn C. W. Lovato, Zilda C. Gazim, José E. Gonçalves, Daisy L. R. Monzon, and Glacy J. da Silva
Springer Science and Business Media LLC



Ethnomedicinal, phytochemical and pharmacological investigations of Baccharis dracunculifolia DC. (ASTERACEAE)
Zilda Cristiani Gazim, Juliana Silveira Valle, Isabela Carvalho dos Santos, Isabelle Luiz Rahal, Gabriela Catuzo Canonico Silva, Ana Daniela Lopes, Suelen Pereira Ruiz, Maria Graciela Iecher Faria, Ranulfo Piau Junior, and Daniela Dib Gonçalves
Frontiers Media SA
Baccharis dracunculifolia DC (Lamiaceae) (Asteraceae) is found in South America, mainly in Argentina, Brazil, Bolivia, Paraguay and Uruguay. Folk medicine is used as a sedative, hypotensive, bronchodilator, cardiovascular disorders, anti-flu, and also in skin wounds. Considered the main source of green propolis, which increases the pharmacological interest in this species. It is also known as a “benefactor” plant facilitating the development of other plant species around it, being indicated for the recovery of degraded areas. This species has been studied for decades in order to isolate and identify the active principles present in the aerial parts (leaves and flowers) and roots. The present study consists of a review of the scientific literature addressing the ethnobotanical, ethnomedicinal, phytochemical, pharmacological and potential cytotoxic effects of the B. dracunculifolia species. In this survey, we sought to investigate issues related to the botanical and geographic description of the species, the ethnobotanical uses, as well as the phytochemical studies of the essential oil, extracts and green propolis obtained from the aerial parts and roots of B. dracunculifolia. Using high precision analytical tools, numerous compounds have already been isolated and identified from leaves and flowers such as the flavonoids: naringenin, acacetin, dihydrokaempferol, isosakuranetin and kaempferide; phenolic acids: p-coumaric, dihydrocoumaric, ferulic (E)-cinnamic, hydroxycinnamic, gallic, caffeic, and several caffeoylquinic acids derivatives; phenolic acids prenylated: artepillin C, baccharin, drupanin; the glycosides dracuculifosides and the pentacyclic triterpenoids: Baccharis oxide and friedelanol. The predominant class in the essential oil of leaves and flowers are terpenoids comprising oxygenated monoterpenes and sesquiterpenes, highlighting the compounds nerolidol, spathulenol, germacrene D and bicyclogermacrene. These compounds give the species high antimicrobial, antioxidant, antitumor, analgesic, immunomodulatory and antiparasitic potential, making this species a promising herbal medicine. In vitro toxicity assays with B. dracunculifolia extract showed low or no cytotoxicity. However, in vivo analyses with high doses of the aqueous extract resulted in genotoxic effects, which leads us to conclude that the toxicity of this plant is dose-dependent.


Bioactive compounds with antifungal activity against pathogens isolated from pregnant woman: Gallesia integrifolia (garlic wood) is a promising treatment for vulvovaginal candidiasis: Therapeutic potential of Gallesia integrifolia for vulvovaginal candidiasis in pregnant woman
Amanda Nascimento Vasques de Souza, Maria Graciela Iecher Faria, Cristiane Edna da Rocha, Gisele Strieder Philippsen, Gabriela Catuzo Canonico Silva, Gustavo Ratti da Silva, Rodrigo Sadao Inumaro, José Eduardo Gonçalves, Zilda Cristiani Gazim, Samantha Wietzikoski,et al.
Elsevier BV



Editorial: Ethnopharmacology of the lamiaceae: Opportunities and challenges for developing new medicines
Sujogya Kumar Panda, Luc Van Puyvelde, Marie Jeanne Mukazayire, and Zilda Cristiani Gazim
Frontiers Media SA
Centre of Environment Climate Change and Public Health, RUSA, Utkal University, Bhubaneswar, India, Department of Biology, Animal Physiology and Neurobiology Section, KU Leuven, Leuven, Belgium, Department of Pharmacy, School of Pharmacy and Medicine, College of Medicine and Health Sciences, University of Rwanda, Huye, Rwanda, Graduate Program in Animal Science and Biotechnology Applied to Agriculture, Chemistry Laboratory of Natural Products, Paranaense University, Umuarama, Brazil


Impact of a Novel Homeopathic Complex Medicine on the Management of Multiple Antibiotic-Resistant Bovine Mastitis: An Open-Label, Non-Randomized, Placebo-Controlled Trial
Bruna de Fatima Antunes Laginestra, Thaís Camaso de Sá, Jessé Lahos Borges, Matheus Basilio Marçal, João Vitor Morais Simões, Selma Alves Rodrigues, Zilda Cristiani Gazim, and Ranulfo Piau Júnior
Georg Thieme Verlag KG
Abstract Background Bovine mastitis is characterized by an inflammatory process in the mammary gland and represents one of the main diseases affecting a dairy herd. Management of mastitis is most commonly via antibiotics, but the rising incidence of multiple antibiotic resistance (MAR) means that additional options are needed. Homeopathic products can be administered in dairy farming for a range of clinical reasons and may be preferential due to the absence of residues. Objectives The aim of this study was to assess the potential of a novel homeopathic complex medicine in managing bovine mastitis. Methods Twenty-four lactating Holstein cows with mastitis were divided into two groups: the homeopathic complex group received a homeopathic complex daily for 60 days at a dose of 20 g/d; the placebo group received the calcium carbonate vehicle without homeopathic medicines at the same dose and repetition. The main outcome measure was somatic cell count (SCC; cells/mL), with additional outcome measures including milk production (kg/d), milk constituents (percentage of protein, fat, lactose and total milk solids), and serum levels of cortisol, glucose, ammonia and lactic acid. All outcomes were measured at the beginning of the study and after 30 and 60 days. Milk samples were also collected from all animals at the beginning of the study, confirming a high (>0.2) MAR index for isolated bacterial cultures. Results Assessment of SCC showed a statistically significant difference favoring the homeopathic complex versus placebo group at day 60. A reduction in serum cortisol levels and an increase in fat, lactose and total milk solids in animals treated with the homeopathic complex at day 60 were also seen. Other outcome measures did not show statistically significant inter-group differences. Conclusion The results of this non-randomized, open-label, placebo-controlled trial suggest the potential for a novel homeopathic complex medicine in management of multiple antibiotic-resistant bovine mastitis, thus offering dairy farmers an additional option to antibiotics and making dairy products safer for consumer health and milk production more sustainable.


Ethnomedicinal, Phytochemical and Pharmacological Investigations of Tetradenia riparia (Hochst.) Codd (Lamiaceae)
Sujogya Kumar Panda, Zilda Cristiani Gazim, Shasank S. Swain, Marisa Cassia Vieira de Araujo Bento, Jéssica da Silva Sena, Marie Jeanne Mukazayire, Luc Van Puyvelde, and Walter Luyten
Frontiers Media SA
Tetradenia ripariaHochsteter codd. (Lamiaceae) in its native African continent, is considered one of the most popular aromatic medicinal plants. In folk medicine it may be used as an infusion to treat respiratory problems, cough, headache, stomach pain, diarrhea, fever, malaria, and dengue; and in the form of compresses it is applied for the relief of headaches and toothaches. The speciesT. ripariahas been researched for decades to isolate and identify chemical constituents present in extracts or essential oil obtained from the leaves, floral buds, or stems of this plant. The present study reviews the scientific literature on ethnomedicinal, phytochemical, and pharmacological aspects ofT. riparia. We discuss issues related to the botanical and geographical description of the species, ethnobotanical uses, phytochemical studies on its essential oil and extracts, and biological activities ofT. riparia.Several compounds have already been isolated from leaves, such as ibozol, 7α-hydroxyroileanone, 1′,2′-dideacetylboronolide, 8(14),15-sandaracopimaradiene-7α,18-diol; 5,6-dihydro-α-pyrone and α-pyrone. Terpenes predominated in the essential oil, comprising monoterpenes, sesquiterpenes, diterpenes, hydrocarbons, and oxygenates. Most phytocompounds were isolated from the leaves and flower buds, namely fenchone, 14-hydroxy-9-epi (E)-caryophyllene, 9β, 13β-epoxy-7-abietene, and 6,7-dehydroroileanone. These compounds provide the species a high pharmacological potential, with antimicrobial, antioxidant, antitumor, analgesic, anti-leishmania, anti-tuberculosis, and anti-parasitic activities. Therefore, this species is a promising herbal medicine.

RECENT SCHOLAR PUBLICATIONS

BIOFORTIFICAO DE LTIO, SELNIO E ZINCO EM BASIDIOMICETOS: UMA NOVA OPO DE SUPLEMENTAO
FPA da Silva, JLPR Servantes, GAR Beiro, ZC Gazim, JS do Valle, ...
Arquivos de Cincias da Sade da UNIPAR 28 (3), 713-736 2024



Homeopatia e produtos bioativos em dietas de frangos de corte
JVM Simes, SA Rodrigues, JPTP Borges, BFA Laginestra, ...
REVISTA DELOS 17 (62), e3214-e3214 2024



Atividade antimicrobiana do leo essencial das folhas tetradenia riparia e seu potencial no combate as bactrias causadores de mastite em bovinos
BFA Laginestra, PW Meskiv, JVM Simes, GR Macagnan, SA Rodrigues, ...
Arquivos de Cincias da Sade da UNIPAR 28 (2), 273-287 2024



Volatile compound changes at Brunfelsia uniflora flower senescence
NB Colauto, HLM de Oliveira, RS Inumaro, FC de Almeida, ...
Cincia e Natura 46, e83735-e83735 2024



Ageratum conyzoides, an invasive species with antioxidant and antifungal potential
VSR Pereira, GCC Silva, EB da Cruz Oliveira, M de Almeida Machado, ...
Cincia e Natura 46, e83759-e83759 2024



ESTUDO DAS ATIVIDADES ANTIMICROBIANAS DE LEOS ESSENCIAIS E SUAS ASSOCIAES NO CONTROLE DA MASTITE EM BOVINOS
JL Borges, PW Meskiv, BFA Laginestra, JVM Simes, GR Macagnan, ...
ARAC 6 (3), 10535-10553 2024



Effects of Baccharis dracunculifolia DC on an Innovative Animal Model of Cardiometabolic Syndrome
GR Silva, AJ Kluck, ER Albuquerque, LP Guarnier, FA Braga, EP Silva, ...
Pharmaceutics 16 (11), 1446 2024



Probiotics and prebiotics in dairy farming
ZC Gazim, AFB De Almada, MDA Machado, SA Rodrigues, JPTP Borges, ...
2024



Genomics Evolution of Ethnomedicinal Plants in a Changing Environment
TB dos Santos, ZC Gazim, HM Magalhes, SGH de Souza
Ethnomedicinal Plants for Drug Discovery: Current Developments, 297-321 2024



Antioxidant activity, antiproliferative activity, antiviral activity, no production inhibition, and chemical composition of essential oils and crude extracts of leaves, flower
JS Sena, SA Rodrigues, K Sakumoto, RS Inumaro, ...
Pharmaceuticals 17 (7), 888 2024



Bioactives of the essential oil from the leaves of Eugenia pyriformis Cambess (Myrtaceae) on the effects of tobacco
JP de Medeiros, SA Rodrigues, K Sakumoto, SP Ruiz, MGI Faria, ...
Frontiers in Pharmacology 15, 1415659 2024



Pachira aquatica: biological activity and chemical composition of leaves, flowers, and seeds
IL Rahal, AB Dias, GC Canonico Silva, MC Vieira de Arajo Bento, ...
Natural Product Research, 1-6 2024



Versatile natural molluscicides against the snail Biomphalaria glabrata Say, 1818 (Pulmonata: Planorbidae), the principal vector of Schistosoma mansoni on the American continent
DB Hartmann, RA Marim, G Zardeto, I de Azevedo Silva, E Jacomassi, ...
2024



Impact of a Novel Homeopathic Complex Medicine on the Management of Multiple Antibiotic-Resistant Bovine Mastitis: An Open-Label, Non-Randomized, Placebo-Controlled Trial
BFA Laginestra, TC de S, JL Borges, MB Maral, JVM Simes, ...
Homeopathy 113 (01), 025-031 2024



Essential Oil from the Leaves, Fruits and Twigs of Schinus terebinthifolius: Chemical Composition, Antioxidant and Antibacterial Potential
KC Oliveira, LMSS Franciscato, SS Mendes, FMA Barizon, DD Gonalves, ...
Molecules 29 (2), 469 2024



Methyl jasmonate and copper activate volatiles and antioxidant mechanisms in'Grecco a Palla'basil produced in vitro
F Grski, GM Gerotti, JE Gonalves, ZC Gazim, HM Magalhes
Journal of Crop Science and Biotechnology 26 (5), 615-629 2023



Development, characterization and anti-Mycobacterium tuberculosis activity of solid lipid nanoparticles for oral Piper corcovadensis roots extract delivery.
CM Mariano Fernandez, SA Kleinubing, TK Karam, FB Lorenzetti, ...
Boletn Latinoamericano y del Caribe de Plantas Medicinales y Aromticas 22 (6) 2023



Uso de beta-glucano subcutneo no controle da mastite bovina
V dos Santos Silva, M de Almeida Machado, SA Rodrigues, JPTP Borges, ...
Peer Review 5 (17), 393-415 2023



Avaliao das atividades antibacteriana e antioxidante do leo essencial do ara-roxo (Psidium rufum, Myrtaceae)
LA Branco, BFA Laginestra, MB Maral, ZC Gazim, IL Rahal, SPR Herrig, ...
Arquivo Brasileiro de Medicina Veterinria e Zootecnia 75, 612-622 2023



Evaluation of antibacterial and antioxidant activity of purple ara essential oil (Psidium rufum, Myrtaceae)
LA Branco, BFA Laginestra, MB Maral, ZC Gazim, IL Rahal, SPR Herrig, ...
Arquivo Brasileiro de Medicina Veterinria e Zootecnia 75, 612-622 2023

MOST CITED SCHOLAR PUBLICATIONS

Antifungal activity of the essential oil from Calendula officinalis L.(Asteraceae) growing in Brazil
ZC Gazim, CM Rezende, SR Fraga, TIE Svidzinski, DAG Cortez
Brazilian Journal of Microbiology 39, 61-63 2008
Citations: 187



Seasonal Variation, Chemical Composition, and Analgesic and Antimicrobial Activities of the Essential Oil from Leaves of Tetradenia riparia (Hochst.) Codd in
ZC Gazim, ACL Amorim, AMC Hovell, CM Rezende, IA Nascimento, ...
Molecules 15 (8), 5509-5524 2010
Citations: 122



Antimicrobial Activity of Essential Oil of Baccharis dracunculifolia DC (Asteraceae) Aerial Parts at Flowering Period
LN Cazella, J Glamoclija, M Soković, JE Gonalves, GA Linde, ...
Frontiers in plant science 10, 27 2019
Citations: 104



Acaricidal activity of the essential oil from Tetradenia riparia (Lamiaceae) on the cattle tick Rhipicephalus (Boophilus) microplus (Acari; Ixodidae)
ZC Gazim, IG Demarchi, MVC Lonardoni, ACL Amorim, AMC Hovell, ...
Experimental parasitology 129 (2), 175-178 2011
Citations: 103



Antifungal and antibacterial activities of Petroselinum crispum essential oil
GA Linde, ZC Gazim, BK Cardoso, LF Jorge, V Tešević, J Glamočlija, ...
Genetics and Molecular Research 15 (3) 2016
Citations: 94



Plant growth and essential oil content of Mentha crispa inoculated with arbuscular mycorrhizal fungi under different levels of phosphorus
RC Urcoviche, ZC Gazim, DC Dragunski, FG Barcellos, O Alberton
Industrial Crops and Products 67, 103-107 2015
Citations: 92



Analysis of the essential oils from Calendula officinalis growing in Brazil using three different extraction procedures
ZC Gazim, CM Rezende, SR Fraga, BP Dias Filho, CV Nakamura, ...
Revista Brasileira de Cincias Farmacuticas 44, 391-395 2008
Citations: 90



Antifungal activity, yield, and composition of Ocimum gratissimum essential oil
FBM Mohr, C Lermen, ZC Gazim, JE Gonalves, O Alberton
Genet. Mol. Res 16 (1), 1-10 2017
Citations: 79



New Natural Diterpene-Type Abietane from Tetradenia riparia Essential Oil with Cytotoxic and Antioxidant Activities
ZC Gazim, F Rodrigues, ACL Amorin, CM de Rezende, M Soković, ...
Molecules 19 (1), 514-524 2014
Citations: 79



Essential oil content and chemical composition of Cymbopogon citratus inoculated with arbuscular mycorrhizal fungi under different levels of lead
C Lermen, F Morelli, ZC Gazim, AP da Silva, JE Goncalves, DC Dragunski, ...
Industrial Crops and Products 76, 734-738 2015
Citations: 75



Determinao do rendimento e atividade antimicrobiana do leo essencial de Cymbopogon citratus (DC.) Stapf em funo de sazonalidade e consorciamento
A Santos, RH Paduan, ZC Gazin, E Jacomassi, PS D'Oliveira, DAG Cortez, ...
Revista Brasileira de Farmacognosia 19, 436-441 2009
Citations: 70



Phytoaccumulation and effect of lead on yield and chemical composition of Mentha crispa essential oil
RA S, RA S, O Alberton, ZC Gazim, A Laverde Jr, J Caetano, ...
Desalination and Water Treatment 53 (11), 3007-3017 2015
Citations: 62



Quimiotipos, Extraccin, Composicin y Aplicaciones del Aceite Esencial de Lippia alba
GA Linde, NB Colauto, E Albert, ZC Gazim
Revista Brasileira de Plantas Medicinais 18 (1), 191-200 2016
Citations: 61



Antileishmanial activity of essential oil and 6, 7-dehydroroyleanone isolated from Tetradenia riparia
IG Demarchi, MV Thomazella, M de Souza Terron, L Lopes, ZC Gazim, ...
Experimental Parasitology 157, 128-137 2015
Citations: 58



Anti-Mycobacterium tuberculosis activity of essential oil and 6, 7-dehydroroyleanone isolated from leaves of Tetradenia riparia (Hochst.) Codd (Lamiaceae)
VP Baldin, RB de Lima Scodro, MA Lopes-Ortiz, AL de Almeida, ...
Phytomedicine 47, 34-39 2018
Citations: 51



Antioxidant activity of α and β-amyrin isolated from Myrcianthes pungens leaves
B Karen Cardoso, H Line Marko de Oliveira, U Zonta Melo, ...
Natural product research 34 (12), 1777-1781 2020
Citations: 43



Antifungal activity of Gallesia integrifolia fruit essential oil
KF Raimundo, WC Bortolucci, J Glamočlija, M Soković, JE Gonalves, ...
brazilian journal of microbiology 49 (suppl 1), 229-235 2018
Citations: 40



Antileishmanial activity of the essential oil from Tetradenia riparia obtained in different seasons
BM Cardoso, TFP Mello, SN Lopes, IG Demarchi, DSL Lera, RB Pedroso, ...
Memrias do Instituto Oswaldo Cruz 110 (8), 1024-1034 2015
Citations: 39



Changes in growth, oxidative metabolism and essential oil composition of lemon balm ('Melissa officinalis' L.) subjected to salt stress
C Bonacina, CB Trevizan, J Stracieri, TB dos Santos, JE Gonalves, ...
Australian Journal of Crop Science 11 (12), 1665-1674 2017
Citations: 38



Effects of copper sulphate (CuSO4) elicitation on the chemical constitution of volatile compounds and the in vitro development of Basil
JR Trettel, ZC Gazim, JE Goncalves, J Stracieri, HM Magalhaes
Scientia Horticulturae 234, 19-26 2018
Citations: 37