Germination of Lepidium sativum seeds and regulation of betagalactosidase enzyme production in response to different abiotic factors Bulgarian Journal of Agricultural Science, 2025
Silver Nanoparticles and Biostimulants Affect Chemical Constituents, Total Phenolics, Antioxidants, and Potential Antimicrobial Activities of Santolina chamaecyparissus Ezz Al-Dein Al-Ramamneh, Khalid Y. Alsharafa, Taha Rababah, Razan J. Rahahleh, Fuad Al-Rimawi, et al. Horticulturae, 2024 The beneficial application of silver nanoparticles and biostimulants to increase crop yield and quality is a long-term strategy to achieve desired agricultural productions that are resilient to various biotic and abiotic challenges. This project aimed to evaluate the individual effects of silver nanoparticles (AgNPs), Ascophyllum nodosum (SEW), and Spirulina platensis (SP) on the growth and physiological responses of Santolina chamaecyparissus. S. chamaecyparissus plants were exposed to AgNPs (20, 40, and 60 mg L−1), SWE (0.5% and 1%), and SP (1%, 2%, and 3%). The finding indicates that the light-harvesting efficiency and plant photochemical capacity are not affected by most treatments except for 60 mg L−1 AgNPs. Furthermore, the pattern of H2O2 levels in leaves was significantly higher after AgNP, SP, and SEW treatments. In parallel, total phenolic production was at least accompanied by a burst in H2O2 levels. However, higher antioxidant activity compared to the control, is shown by the higher free-DPPH-radical inhibition that goes completely smoothly with lower H2O2 levels. Thus, the results of the present study showed that biostimulants overall improved the antioxidant activity of S. chamaecyparissus and induced variable detectable amounts of phenolic compounds in response to the concentrations of each biostimulant.
Comparative Coexpression Analysis of Indole Synthase and Tryptophan Synthase A Reveals the Independent Production of Auxin via the Cytosolic Free Indole Yousef M. Abu-Zaitoon, Ezz Al-Dein Muhammed Al-Ramamneh, Abdel Rahman Al Tawaha, Sulaiman M. Alnaimat, Fouad A. Almomani Plants, 2023 Indole synthase (INS), a homologous cytosolic enzyme of the plastidal tryptophan synthase A (TSA), has been reported as the first enzyme in the tryptophan-independent pathway of auxin synthesis. This suggestion was challenged as INS or its free indole product may interact with tryptophan synthase B (TSB) and, therefore, with the tryptophan-dependent pathway. Thus, the main aim of this research was to find out whether INS is involved in the tryptophan-dependent or independent pathway. The gene coexpression approach is widely recognized as an efficient tool to uncover functionally related genes. Coexpression data presented here were supported by both RNAseq and microarray platforms and, hence, considered reliable. Coexpression meta-analyses of Arabidopsis genome was implemented to compare between the coexpression of TSA and INS with all genes involved in the production of tryptophan via the chorismate pathway. Tryptophan synthase A was found to be coexpressed strongly with TSB1/2, anthranilate synthase A1/B1, phosphoribosyl anthranilate transferase1, as well as indole-3-glycerol phosphate synthase1. However, INS was not found to be coexpressed with any target genes suggesting that it may exclusively and independently be involved in the tryptophan-independent pathway. Additionally, annotation of examined genes as ubiquitous or differentially expressed were described and subunits-encoded genes available for the assembly of tryptophan and anthranilate synthase complex were suggested. The most probable TSB subunits expected to interact with TSA is TSB1 then TSB2. Whereas TSB3 is only used under limited hormone conditions to assemble tryptophan synthase complex, putative TSB4 is not expected to be involved in the plastidial synthesis of tryptophan in Arabidopsis.
Foliar Application of Moringa Leaf Extracts Affects Growth, Yield and Mineral Composition of Pepper (Capsicum Annuum L.) under Greenhouse Conditions Arwa Mehdawe, Atif Mahadeen, Ezz Al-Dein Al-Ramamneh Journal of Ecological Engineering, 2023 The higher cost and increased pollution caused by the intensive use of fertilizers in growing vegetables necessitates the use of safer organic bio-stimulants to partially substitute fertilizers. Analyses of leaf extracts of Moringa oleifera and Moringa peregrina in the present study confirmed their rich content of diverse compounds and ele - ments and indicated that except for Mg and Ca, M. oleifera outyielded M. peregrina for the measured elements and bio-constituents. Foliar spray of leaf extracts of M. oleifera and M. peregrina at (1:10, 1:20, 1:30 extract: distilled water) was attempted on pepper plants under greenhouse conditions every two weeks for five times start - ing 30 days after transplanting date. Enhanced vegetative growth parameters (plant height, number of branches and leaves, plant fresh and dry weights, leaf area, and leaf chlorophyll-content) and better yield attributes (Fruit yield·plant -1 and hectare -1 ) were obtained by foliar spraying of plants with the concentrated extract 1:10 of moringa species. The vegetative growth of pepper plants was significantly influenced by extract concentration, moringa type·concentration, but not for moringa type (P≤0.05). However, moringa type, extract concentration and their interactions significantly affected pepper fruit-yield·plant -1 , pepper fruit-yield·ha -1 and mineral content of pepper leaves (P≤0.05). Irrespective of moringa type, the highest extract concentration 1:10 resulted in the highest yield ·plant -1 (1.68 kg) and yield·hectare -1 (16.88 ton) of peppers. The present study highlighted the potential of using extracts of moringa trees in organic farming.
Interaction between zinc and selenium bio-fortification and toxic metals (loid) accumulation in food crops Mina Bayanati, Abdel Rahman Al-Tawaha, Duraid Al-Taey, Abdul Latief Al-Ghzawi, Yousef M. Abu-Zaitoon, et al. Frontiers in Plant Science, 2022 Biofortification is the supply of micronutrients required for humans and livestock by various methods in the field, which include both farming and breeding methods and are referred to as short-term and long-term solutions, respectively. The presence of essential and non-essential elements in the atmosphere, soil, and water in large quantities can cause serious problems for living organisms. Knowledge about plant interactions with toxic metals such as cadmium (Cd), mercury (Hg), nickel (Ni), and lead (Pb), is not only important for a healthy environment, but also for reducing the risks of metals entering the food chain. Biofortification of zinc (Zn) and selenium (Se) is very significant in reducing the effects of toxic metals, especially on major food chain products such as wheat and rice. The findings show that Zn- biofortification by transgenic technique has reduced the accumulation of Cd in shoots and grains of rice, and also increased Se levels lead to the formation of insoluble complexes with Hg and Cd. We have highlighted the role of Se and Zn in the reaction to toxic metals and the importance of modifying their levels in improving dietary micronutrients. In addition, cultivar selection is an essential step that should be considered not only to maintain but also to improve the efficiency of Zn and Se use, which should be considered more climate, soil type, organic matter content, and inherent soil fertility. Also, in this review, the role of medicinal plants in the accumulation of heavy metals has been mentioned, and these plants can be considered in line with programs to improve biological enrichment, on the other hand, metallothioneins genes can be used in the program biofortification as grantors of resistance to heavy metals.
Efficacy of Sterculia diversifolia Leaf Extracts: Volatile Compounds, Antioxidant and Anti-Inflammatory Activity, and Green Synthesis of Potential Antibacterial Silver Nanoparticles Ezz Al-Dein M. Al-Ramamneh, Ayoup M. Ghrair, Ashok K. Shakya, Khalid Y. Alsharafa, Khalid Al-Ismail, et al. Plants, 2022 Sterculia diversifolia, widely distributed in Jordan as an ornamental plant, is a synonoum for Brachychiton populneus. Phytochemical studies examining the volatile chemicals in Sterculia diversifolia leaves are limited, despite the rising demand for their numerous applications. Furthermore, it was only recently that a report described the friendly synthesis of silver nanoparticles (AgNPs) using aqueous extract derived from Brachychiton populneus leaves. Therefore, AgNPs were produced using either aqueous plant extracts (AgWPE) or ethanolic plant extracts (AgEPE), and Shimadzu GC-MS equipment was used to detect volatile compounds in the ethanolic leaf extracts. GC-MS profile of leaf ethanolic extracts of the Jordanian chemotypes of S. diversifolia revealed the existence of major components: (3β)-Lup-20(29)-en-3-ol acetate (30.97%) and 1-octadecyne (24.88). Other compounds are squalene (7.19%), germanicol (6.23), dl-α-tocopherol (5.24), heptacosane (4.41), phytol (3.54) and pentacosane (2.89). According to published studies, these reported chemicals have numerous uses, including as animal feed, vitamin precursors, possible eco-friendly herbicides, antioxidants, and anti-inflammatory agents. Aqueous extracts of S. diversifolia leaves had total phenolic of 5.33 mg GAE/g extract and flavonoid contents of 64.88 mg QE/g extract, respectively. The results indicated the contribution of phenolic and flavonoids to this plant’s anti-inflammatory and antioxidant properties. The reduction in AgNO3 to AgNPs using S. diversifolia leaf extracts was confirmed by the change in solution color from colorless to dark black. Further characterization was attempted by X-ray diffraction, Malvern zeta-sizer and scanning electron microscope. The efficacy of synthesized Ag nanoparticles using aqueous or ethanolic plant extract of S. diversifolia against the Gram-negative bacteria Escherichia coli and Gram-positive bacteria Staphylococcus aureus showed appreciable activity at 25 µg/mL concentration compared to the source plant extracts.
The antimicrobial activity of essential oils and extracts of some medicinal plants grown in Ash-shoubak region - South of Jordan Pakistan Journal of Pharmaceutical Sciences, 2012
The present-day survey of registered and standardized herbal medicines in Jordanian pharmaceutical market American Eurasian Journal of Sustainable Agriculture, 2011
Intercropping effects on growth, yield and biological activity of Thymus vulgaris and Salvia officinalis under open field conditions EAD Al-Ramamneh, Z Al-Rawashdeh, K Muwaffaq, H Aldal’in, S Qaraleh, ... Notulae Scientia Biologicae 18 (2), 12837-12837 , 2026 2026
The Growth and Yield Response of Selected Apricot Cultivars Grown in Mafraq Governate for Two Growing Seasons to Chilling Hours in Northern Jordan EADM Al-Ramamneh, IM Qrunfleh, MT Sadder International Journal of Agriculture and Biosciences 15 (1), 263-271 , 2026 2026
Effect of Ascophyllum nodosum and green-synthesized silver nanoparticles on in vitro axillary shoot proliferation and biochemical profile of Paulownia elongata shoots EAD Al-Ramamneh, I Musallam, T Rababah, A Ghrair, F Al-Rimawi, ... Plant Cell, Tissue and Organ Culture (PCTOC) 163 (2), 72 , 2025 2025
Germination of Lepidium sativum seeds and regulation of beta-galactosidase enzyme production in response to different abiotic factors WZ Alshamaileh, KY Alsharafa, TJ Hijazin, Y Al Hajaya, ... Bulgarian Journal of Agricultural Science 31 (5), 964-975 , 2025 2025
Ascophyllum nodosum and Spirulina platensis affect plant growth, yield, concentration of hormones in the leaves and nematode communities in the rhizosphere of … EADM Al-Ramamneh Biological Agriculture & Horticulture 40 (2), 92-106 , 2024 2024 Citations: 3
Interaction between zinc and selenium bio-fortification and toxic metals (loid) accumulation in food crops (Retraction of Vol 13, art no 1001992, 2022) M Bayanati, AR Al-Tawaha, D Al-Taey, AL Al-Ghzawi, Y Abu-Zaitoon, ... FRONTIERS IN PLANT SCIENCE 15 , 2024 2024
Silver nanoparticles and biostimulants affect chemical constituents, total phenolics, antioxidants, and potential antimicrobial activities of Santolina chamaecyparissus EAD Al-Ramamneh, KY Alsharafa, T Rababah, RJ Rahahleh, ... Horticulturae 10 (1), 26 , 2023 2023 Citations: 5
Comparative coexpression analysis of indole synthase and tryptophan synthase a reveals the independent production of auxin via the cytosolic free indole YM Abu-Zaitoon, EADM Al-Ramamneh, AR Al Tawaha, SM Alnaimat, ... Plants 12 (8), 1687 , 2023 2023 Citations: 10
Foliar application of moringa leaf extracts affects growth, yield and mineral composition of pepper (Capsicum annuum L.) under greenhouse conditions A Mehdawe, A Mahadeen, EAD Al-Ramamneh Journal of Ecological Engineering 24 (6), 329-337 , 2023 2023 Citations: 14
RETRACTED: Interaction between zinc and selenium bio-fortification and toxic metals (loid) accumulation in food crops M Bayanati, AR Al-Tawaha, D Al-Taey, AL Al-Ghzawi, YM Abu-Zaitoon, ... Frontiers in Plant Science 13, 1001992 , 2022 2022 Citations: 13
Efficacy of Sterculia diversifolia Leaf Extracts: Volatile Compounds, Antioxidant and Anti-Inflammatory Activity, and Green Synthesis of Potential Antibacterial Silver … EADM Al-Ramamneh, AM Ghrair, AK Shakya, KY Alsharafa, K Al-Ismail, ... Plants 11 (19), 2492 , 2022 2022 Citations: 20
A comparative study of onion purple blotch (caused by Alternaria porri) and tomato early blight (caused by a. solani) diseases in southern Ghors of Jordan ZB Al-Rawashdeh, MR Karajeh, EADM Al-Ramamneh, ... Jordan Journal of Biological Sciences 15 (1) , 2022 2022 Citations: 2
Plant—Microbe Interaction under Climate Change ARM Al-Tawaha, E Al-Dein-Al-Ramamneh, M Aasim, C Sevinc, ... Developing Climate-Resilient Crops, 159-173 , 2021 2021 Citations: 5
Soil fertility decline under climate change ARM Al-Tawaha, H Günal, J Křeček, RHC Zamfir, HK Patel, RV Vyas, ... Sustainable Soil and Land Management and Climate Change, 127-145 , 2021 2021 Citations: 21
Micronutrient biofortification in rice for better quality Imran, Amanullah, ARMS Al-Tawaha, AR Al Tawaha, AM Qaisi, ... Rice Research for Quality Improvement: Genomics and Genetic Engineering … , 2020 2020 Citations: 5
The response of dill plant morphology and production parameters to different urea application levels when grown in calcareous soil: correlation analysis. GJ Al-Rabadi, SA Al-Dalain, M Al-Rawashdeh, MB Al-Nawaiseh, ... 2020
molecular characterization of jordanian pomegranate (punica granatum l.) genotypes using rapd markers in ma’an region AR Al-Tawaha, M Karajeh Fresenius Environmental Bulleti 29 (12), 11761-7 , 2020 2020 Citations: 4
Growth, yield and biochemical responses in barley to DAP and chitosan application under water stress ARM Al-Tawaha, N Jahan, N Odat, EAD Al-Ramamneh, AR Al-Tawaha, ... Journal of Ecological Engineering 21 (6) , 2020 2020 Citations: 35
Assesment of exogenous application of plant growth regulators on Crees seed germination and β-Galactosidase activity O Alghonmeen, K Al-sharafa, M Allimoun, K Khleifat, E Al-Dein, ... Plant Science Today 7 (2), 257-263 , 2020 2020 Citations: 8
Effects of gamma irradiation on the alkaloid content in seeds of Datura stramonium and the radiosensitivity of derived seedlings N Benslimani, M Khelifi-Slaoui, A Morsli, A Djerrad, EAD Al-Ramamneh, ... Plant Sci. Today 6, 533-540 , 2019 2019 Citations: 3
MOST CITED SCHOLAR PUBLICATIONS
Plant growth strategies of Thymus vulgaris L. in response to population density AL Ezz Industrial Crops and Products 30 (3), 389-394 , 2009 2009 Citations: 99
The antimicrobial activity of essential oils and extracts of some medicinal plants grown in Ash-shoubak region-South of Jordan MS Abu-Darwish, EA Al-Ramamneh, VS Kyslychenko, UV Karpiuk Pak J Pharm Sci 25 (1), 239-246 , 2012 2012 Citations: 74
Using chitosan to improve growth of maize cultivars under salinity conditions. AR Al-Tawaha, MA Turk, ARM Al-Tawaha, MH Alu'Datt, M Wedyan, ... Bulgarian Journal of Agricultural Science 24 (3) , 2018 2018 Citations: 72
Plant regeneration via somatic embryogenesis in Schlumbergera truncata EAD Al-Ramamneh, S Sriskandarajah, M Serek Plant Cell, Tissue and Organ Culture 84 (3), 333-342 , 2006 2006 Citations: 37
Growth, yield and biochemical responses in barley to DAP and chitosan application under water stress ARM Al-Tawaha, N Jahan, N Odat, EAD Al-Ramamneh, AR Al-Tawaha, ... Journal of Ecological Engineering 21 (6) , 2020 2020 Citations: 35
Effects of soil type and rainwater harvesting treatments in the growth, productivity and morphological trains of barley plants cultivated in semi-arid environment AR Al-Tawaha, AR Al-Tawaha, M Alu'datt, AL Al-Ghzawi, M Wedyan, ... Australian journal of crop science 12 (6), 975-979 , 2018 2018 Citations: 34
The influence of plant growth regulators on callus induction from hypocotyls of cucumber (Cucumis sativus L.) S Abu-Romman, M Suwwan, AL Ezz Advances in Environmental Biology, 339-344 , 2013 2013 Citations: 34
The impact of cattle manure on the content of major minerals and nitrogen uptake from 15N isotope-labeled ammonium sulphate fertilizer in maize (Zea mays L.) plants NB Hani, EAD Al-Ramamneh, M Haddad, AR Al-Tawaha, Y Al-Satari Pak. J. Bot 51 (1), 185-189 , 2019 2019 Citations: 27
Propagation physiology of Juniperus phoenicea L. from Jordan using seeds and in vitro culture techniques: Baseline information for a conservation perspective AL Ezz, S Dura, N Daradkeh African Journal of Biotechnology 11 (30), 7684-7692 , 2012 2012 Citations: 25
Drought tolerance mechanisms of pearl millet S Golombek, EAD Al-Ramamneh Conference on Agricultural and Natural Resource Management, 9-11 , 2002 2002 Citations: 24
Soil fertility decline under climate change ARM Al-Tawaha, H Günal, J Křeček, RHC Zamfir, HK Patel, RV Vyas, ... Sustainable Soil and Land Management and Climate Change, 127-145 , 2021 2021 Citations: 21
Efficacy of Sterculia diversifolia Leaf Extracts: Volatile Compounds, Antioxidant and Anti-Inflammatory Activity, and Green Synthesis of Potential Antibacterial Silver … EADM Al-Ramamneh, AM Ghrair, AK Shakya, KY Alsharafa, K Al-Ismail, ... Plants 11 (19), 2492 , 2022 2022 Citations: 20
Foliar application of moringa leaf extracts affects growth, yield and mineral composition of pepper (Capsicum annuum L.) under greenhouse conditions A Mehdawe, A Mahadeen, EAD Al-Ramamneh Journal of Ecological Engineering 24 (6), 329-337 , 2023 2023 Citations: 14
Determination of Essential Oil Bioactive Components and Rosmarinic Acid of Salvia officinalis Cultivated under Different Intra-row Spacing. M ABU DARWISH, EALD AL-RAMAMNEH, I SALAMO, Z ABU-DIEYEH, ... Notulae Scientia Biologicae 5 (2) , 2013 2013 Citations: 14
RETRACTED: Interaction between zinc and selenium bio-fortification and toxic metals (loid) accumulation in food crops M Bayanati, AR Al-Tawaha, D Al-Taey, AL Al-Ghzawi, YM Abu-Zaitoon, ... Frontiers in Plant Science 13, 1001992 , 2022 2022 Citations: 13
Occurrence and Control of Strawberry Powdery Mildew in Al-Shoubak/Jordan. MR Karajeh, ZB Al-Rawashdeh, EADM Al-Ramamneh Jordan Journal of Agricultural Sciences 8 (3) , 2012 2012 Citations: 13
Agrobacterium tumefaciens -mediated transformation of Rhipsalidopsis gaertneri EA Al-Ramamneh, S Sriskandarajah, M Serek Plant Cell Reports 25 (11), 1219-1225 , 2006 2006 Citations: 13
Effects of explant, media and growth regulators on in vitro regeneration and antioxidant activity of Juniperus phoenicea EAD Al-Ramamneh, N Daradkeh, T Rababah, D Pacurar, M Al-Qudah Australian Journal of Crop Science 11 (7), 828 , 2017 2017 Citations: 12
Plant response of strawberry to intra-row spacing and growing conditions in south of Jordan. E Al-Ramamneh, Z Al-Rawashdeh, M Karajeh, S Abu-Romman 2013 Citations: 11
Comparative coexpression analysis of indole synthase and tryptophan synthase a reveals the independent production of auxin via the cytosolic free indole YM Abu-Zaitoon, EADM Al-Ramamneh, AR Al Tawaha, SM Alnaimat, ... Plants 12 (8), 1687 , 2023 2023 Citations: 10
