Abhay Kumar
@nrclitchi.icar.gov.in
Senior Scientist
ICAR-National Research Centre on Litchi
EDUCATION
PhD (Molecular Biology and Biotechnology)
RESEARCH, TEACHING, or OTHER INTERESTS
Agricultural and Biological Sciences, Biochemistry, Genetics and Molecular Biology, General Agricultural and Biological Sciences
Scopus Publications
Scopus Publications
Narayan Lal, Awtar Singh, Abhay Kumar, E. S. Marboh, A. K. Gupta, Alemwati Pongener, Vishal Nath, and S. D. Pandey
Springer Science and Business Media LLC
Narayan Lal, Awtar Singh, Abhay Kumar, and Sheshdhar Pandey
Springer Science and Business Media LLC
Narayan Lal, Abhay Kumar, E. S. Marboh, A. K. Gupta, Vishal Nath, and S. D. Pandey
Springer Science and Business Media LLC
Narayan Lal, Abhay Kumar, Awtar Singh, ES Marboh, AK Gupta, SD Pandey, and Vishal Nath
Springer Science and Business Media LLC
Narayan Lal, Abhay Kumar, Sheshdhar Pandey, and Vishal Nath
Springer Science and Business Media LLC
Narayan Lal, Awtar Singh, S.K. Singh, Abhay Kumar, S.D. Pandey, and Vishal Nath
Indian Academy of Horticultural Sciences
Twenty nine quantitative phenological traits were used to assess the morphological diversity among30 Indianlitchi genotypes. Coefficients of variation for length and width of panicle, number of primary and secondarydichasia, maximum length of primary and secondary dichasia, maximum and minimum distance between twoprimary dichasia and origin of number of primary dichasia (primary branches on panicle) from same point,filament length, length and diameter of anther, number of fruits/cluster and yield/ plant were more than 20%.The number of secondary dichasia was highly correlated with the length of stigma. Similarly, number of fruitsper cluster and yield, and fruit weight and thickness of pedicle were also correlated. Genotypes were dividedinto two main groups which corresponding to the size of the panicle and yield. Cluster-I contained genotypesof comparatively small panicle with lower yield, while cluster-II is composed of larger panicle with higheryield. From variability analysis, length and width of panicle, number of secondary dichasia, maximum lengthof primary dichasia, length of male flower, stamen length, thickness of pedicle, number of fruits/cluster andfruit weight were identified as important traits for identifying high-yielding genotypes.
Narayan Lal Abhay Kumar and SD Pandey
Bangladesh Academy of Sciences
Twenty five morphological traits of tree trunk, branch and leaf were employed to discriminate 30 Indian Litchi (Litchi chinensis Sonn.) genotypes as well as to assess their morphological diversity at ICAR-NRC on Litchi, Muzaffarpur, India. Litchi genotypes were highly diversified. Twelve qualitative and 13 quantitative traits exhibited high degrees of variability. High phenotypic and genotypic coefficients of variation were recorded for tree volume (31.26 and 31.15%), leaf area (22.27 and 21.87%) and thickness of leaf (21.56 and 20.89%). High heritability coupled with high genetic advances was recorded for plant height (97.92%), crown diameter (99.40%), tree volume (99.34%), leaflet number (93.28%), rachis length (91.45%), petiole length (96.45%), petiolule length (95.91%), leaf length (93.07%), leaf area (96.39%) and leaf thickness (93.89%), indicating ample scope of improvement for these traits through selection. Bangladesh J. Bot. 52(1): 197-202, 2023 (March)
Narayan Lal, Alemwati Pongener, Abhay Kumar, and S. D. Pandey
Springer Science and Business Media LLC
N. Kiran Kumar, A.R. Nirmal Kumar, B.V. Bhaskar Reddy, and L. Prasanthi
Agricultural Research Communication Center
Background: Dry root rot (DRR), yellow mosaic disease (YMD) and stem necrosis diseases are inflicting the economic losses in urdbean. Information on the interrelation of morphological and biochemical parameters of the urdbean cultivars with the disease resistance is scarce. Methods: Thirty nine cultivars of urdbean were screened in the field for their resistance to DRR, YMD and stem necrosis diseases during Rabi, 2019-2020. The incidence of the diseases is compared with the morphological characters viz., trichome density, leaf thickness, root thickness and biochemical composition viz., total free amino acids, total soluble sugars and phenol content of cultivars. Result: Cultivar GBG 103 was found resistant to DRR and YMD with the maximum amount of trichome density. No cultivar was found resistant to stem necrosis under field conditions. Correlation analysis revealed the positive correlation of phenol content with the DRR incidence; trichome density and total soluble sugars with YMD incidence. Albeit the leaf thickness and total free amino acid content of the cultivars were negatively correlated with the incidence of the DRR, YMD and stem necrosis diseases.
T. N. V. K. V. Prasad, G. C. Satisha, A. R. Nirmal Kumar, M. Swethasree, B. P. Girish, P. Sudhakar, B. Ravindra Reddy, M. Saritha, N. Sabitha, B. V. Bhaskar Reddy,et al.
American Chemical Society (ACS)
NARAYAN LAL, NISHA SAHU, ABHAY KUMAR, and SHESHDHAR PANDEY
Association of Agrometeorologists
The sun burn and fruit cracking in litchi were high in 2019 (10.5% and 10.1%) when temperature was high with low rainfall during fruit growth while low in 2017 (1.9% and 3.7%) when temperature was low. Lack of soil moisture and high temperature reduced the fruit weight in Kasba cultivar by (26.2%) and rainfall induced cracking in the cultivars Bedana and Early Bedana. The cultivars having higher relative water content and cuticle thickness reflected in low fruit cracking and vice-versa. These results may be helpful to identify suitable cultivars for preventive measures to reduce sun burn and fruit cracking in litchi.
Narayan Lal, Nisha Sahu, Evening Stone Marboh, Alok Kumar Gupta, Abhay Kumar, and Vishal Nath
Springer Science and Business Media LLC
Narayan Lal, Alok Kumar Gupta, Evening Stone Marboh, Abhay Kumar, and Vishal Nath
Springer Science and Business Media LLC
Vivekanand Tiwari, Abhay Kumar, and Pratibha Singh
Wiley
Reena Yadav, Radhakrishnan Thankappan, and Abhay Kumar
Springer Singapore
Tejas C. Bosamia, Sneha M. Dodia, Gyan P. Mishra, Suhail Ahmad, Binal Joshi, Polavakkalipalayam P. Thirumalaisamy, Narendra Kumar, Arulthambi L. Rathnakumar, Chandramohan Sangh, Abhay Kumar,et al.
Public Library of Science (PLoS)
Stem rot, a devastating fungal disease of peanut, is caused by Sclerotium rolfsii. RNA-sequencing approaches have been used to unravel the mechanisms of resistance to stem rot in peanut over the course of fungal infection in resistant (NRCG-CS85) and susceptible (TG37A) genotypes under control conditions and during the course of infection. Out of about 290 million reads, nearly 251 million (92.22%) high-quality reads were obtained and aligned to the Arachis duranensis and Arachis ipaensis genomes with the average mapping of 78.91% and 78.61%, respectively. In total, about 48.6% of genes were commonly regulated, while approximately 21.8% and 29.6% of uniquely regulated genes from A. duranensis and A. ipaensis genomes, respectively, were identified. Several annotated transcripts, such as receptor-like kinases, jasmonic acid pathway enzymes, and transcription factors (TFs), including WRKY, Zinc finger protein, and C2-H2 zinc finger, showed higher expression in resistant genotypes upon infection. These transcripts have a known role in channelizing the downstream of pathogen perception. The higher expression of WRKY transcripts might have induced the systemic acquired resistance (SAR) by the activation of the jasmonic acid defense signaling pathway. Furthermore, a set of 30 transcripts involved in the defense mechanisms were validated with quantitative real-time PCR. This study suggested PAMP-triggered immunity as a probable mechanism of resistance, while the jasmonic acid signaling pathway was identified as a possible defense mechanism in peanut. The information generated is of immense importance in developing more effective ways to combat the stem rot disease in peanut.
Abhay Kumar, Vivekanand Tiwari, Pratibha Singh, Sujit Kumar Bishi, Chandan Kumar Gupta, and Gyan Prakash Mishra
Springer Singapore
Narayan Lal, , ES Marboh, AK Gupta, Abhay Kumar, AK Dubedi Anal, Vishal Nath, , , ,et al.
Journal of Experimental Biology and Agricultural Sciences
To assess the role of phenol in flowering of litchi, an experiment was conducted at ICAR-NRC on Litchi, Muzaffarpur. Twenty desired litchi genotypes were selected and content of leaf phenol and leaf flavonoids were estimated from flowering and non-flowering trees. Results revealed that phenol content varied from 22.86 – 53.59 mg/g in flowering tree while it ranged from 10.03 – 33.7 mg/g in non-flowering tree during 2017. Among flowering genotypes phenol content was ranged from 16.51-50.35 mg/g. The highest phenol content was recorded in genotypes IC-0615590 (53.59 mg/g) whereas lowest was found in genotype IC0615589 (22.86 mg/g) during 2017. The difference in phenol content between flowering and non-flowering tree ranged 12.74 66.09 %. The genotype Coll. 39 contained 66.09 % more phenol in flowering tree as compared to non-flowering trees in 2017 and IC-0615597 possessed 12.74% more phenol in flowering tree as compared to non-flowering tree during the same period. Similarly, phenol content ranged from 6.45 – 31.17 mg/g in non-flowering tree in 2018. In 2018, phenol content followed the same trend registering the maximum content in genotype IC-0615590 (50.35 mg/g) and lowest in IC-0615593 (16.51 mg/g). The difference in phenol content between flowering and non-flowering tree in 2018 ranged from 3.27 71.46 %. The genotype IC-0615604 possessed 71.46 % more phenol in flowering tree as compared to nonflowering tree and IC-0615593 contained 3.27 % more phenol in flowering tree as compared to nonflowering tree. In general, it was observed that the level of phenol in litchi tree varied from year to year but flowering tree always possessed more content of phenol as compared to non-flowering trees. However, the relation of flavonoids and flowering in litchi was not observed. * Corresponding author KEYWORDS
Bhagwat Nawade, Gyan P. Mishra, T. Radhakrishnan, Snehaben M. Dodia, Suhail Ahmad, Abhay Kumar, Atul Kumar, and Rahul Kundu
Elsevier BV
Kirankumar G. Patel, Radhakrishnan Thankappan, Gyan P. Mishra, Viralkumar B. Mandaliya, Abhay Kumar, and Jentibhai R. Dobaria
Frontiers Media SA
Peanut, an important oilseed crop, frequently encounters drought stress (DS) during its life cycle. In this study, four previously developed mtlD transgenic (T) peanut lines were used for detailed characterization under DS, at the reproductive stage using lysimeter system under controlled greenhouse conditions. In dry-down experiments, T lines maintained better photosynthetic machinery, such as, photosynthesis rate, stomatal conductance, transpiration rate, and SPAD (Soil-Plant Analyses Development) values, and had lower oxidative damage, including lipid membrane peroxidation and hydrogen peroxide and superoxide radical accumulation than WT, when exposed to 24 days of DS. WT plants had a more negative water potential (WP; up to −3.22 MPa) than T lines did (−2.56 to −2.71 MPa) at day 24 of DS treatment. During recovery, T lines recovered easily whereas 67% of WT plants failed to recover. In T lines, the rate of photosynthesis strongly and positively correlated with the transpiration rate (r = 0.92), RWC (r = 0.90), WP (r = 0.86), and total chlorophyll content (r = 0.75), suggesting its strong correlation with water retention-related parameters. Furthermore, yield parameters such as, pod weight and harvest index of T lines were up to 2.19 and 1.38 times more than those of WT plants, respectively. Thus, the significantly better performance of mtlD T peanut lines than of WT plants under DS could be attributed to the accumulation of mannitol, which in turn helped in maintaining the osmoregulation and ROS scavenging activity of mannitol and ultimately conferred water-economizing capacity and higher yield in T lines than in WT plants.
A.S. Patil, , R. Thankappan, R. Mehta, R. Yadav, A. Kumar, G.P. Mishra, J.R. Dobaria, P.P. Thirumalaisamy, R.K. Jain,et al.
Triveni Enterprises
Bhagwat Nawade, Tejas C. Bosamia, Radhakrishnan Thankappan, Arulthambi L. Rathnakumar, Abhay Kumar, Jentilal R. Dobaria, Rahul Kundu, and Gyan P. Mishra
Frontiers Media SA
In peanut (Arachis hypogaea L.), the customization of fatty acid profile is an evolving area to fulfill the nutritional needs in the modern market. A total of 174 peanut genotypes, including 167 Indian cultivars, 6 advanced breeding lines and “SunOleic95R”—a double mutant line, were investigated using AS-PCRs, CAPS and gene sequencing for the ahFAD2 allele polymorphism, along with its fatty acid compositions. Of these, 80 genotypes were found having substitution (448G>A) mutation only in ahFAD2A gene, while none recorded 1-bp insertion (441_442insA) mutation in ahFAD2B gene. Moreover, 22 wild peanut accessions found lacking both the mutations. Among botanical types, the ahFAD2A mutation was more frequent in ssp. hypogaea (89%) than in ssp. fastigiata (17%). This single allele mutation, found affecting not only oleic to linoleic acid fluxes, but also the composition of other fatty acids in the genotypes studied. Repeated use of a few selected genotypes in the Indian varietal development programs were also eminently reflected in its ahFAD2 allele polymorphism. Absence of known mutations in the wild-relatives indicated the possible origin of these mutations, after the allotetraploidization of cultivated peanut. The SNP analysis of both ahFAD2A and ahFAD2B genes, revealed haplotype diversity of 1.05% and 0.95%, while Ka/Ks ratio of 0.36 and 0.39, respectively, indicating strong purifying selection pressure on these genes. Cluster analysis, using ahFAD2 gene SNPs, showed presence of both mutant and non-mutant genotypes in the same cluster, which might be due the presence of ahFAD2 gene families. This investigation provided insights into the large number of Indian peanut genotypes, covering various aspects related to O/L flux regulation and ahFAD2 gene polymorphism.
Tanmoy Sarkar, Radhakrishnan Thankappan, Abhay Kumar, Gyan P. Mishra, and Jentilal R. Dobaria
Frontiers Media SA
Peanut, an important oilseed crop, is gaining priority for the development of drought tolerant genotypes in recent times, since the area under drought is constantly on the rise. To achieve this, one of the important strategies is to genetically engineer the ruling peanut varieties using transcription factor regulating the expression of several downstream, abiotic-stress responsive gene(s). In this study, eight independent transgenic peanut (cv. GG20) lines were developed using AtDREB1A gene, encoding for a transcription factor, through Agrobacterium-mediated genetic transformation. The transgene insertion was confirmed in (T0) using PCR and Dot-blot analysis, while copy-number(s) was ascertained using Southern-blot analysis. The inheritance of AtDREB1A gene in individual transgenic plants (T1 and T2) was confirmed using PCR. In homozygous transgenic plants (T2), under soil-moisture deficit stress, elevated level of AtDREB1A transgene expression was observed by RT-PCR assay. The transgenic plants at 45-d or reproductive growth stage showed tolerance to severe soil-moisture deficit stress. Physio-biochemical parameters such as proline content, osmotic potential, relative water content, electrolytic leakage, and total-chlorophyll content were found positively correlated with growth-related traits without any morphological abnormality, when compared to wild-type. qPCR analysis revealed consistent increase in expression of AtDREB1A gene under progressive soil-moisture deficit stress in two homozygous transgenic plants. The transgene expression showed significant correlation with improved physio-biochemical traits. The improvement of drought-stress tolerance in combination with improved growth-related traits is very essential criterion for a premium peanut cultivar like GG20, so that marginal farmers of India can incur the economic benefits during seasonal drought and water scarcity.