Tetiana Bondarchuk

@new.meduniv.lviv.ua

biochrmistry
Danylo Halytsky Lviv National Medical University

https://researchid.co/tetianabondarchuk

RESEARCH, TEACHING, or OTHER INTERESTS

Biochemistry, Biochemistry (medical), Biochemistry, Genetics and Molecular Biology, Gastroenterology

Scopus Publications

Scholar Citations

Scholar h-index

Scholar i10-index

Scopus Publications

Anti-inflammatory hydrogen sulfide-releasing agents with reduced gastro- And enterotoxicity on the stress model in rats
Iryna FOMENKO, Iryna LOZYNSKA, Tetyana BONDARCHUK, Natalia DENYSENKO, Roman LESYK, and Alexander SKLYAROV
Edizioni Minerva Medica

Changes of nitric oxide system and lipid peroxidation parameters in the digestive system of rats under conditions of acute stress, and use of nonsteroidal anti-inflammatory drugs
Iryna Fomenko, Tetyana Bondarchuk, Vitaliy Emelyanenko, Natalia Denysenko, Sklyarov Pavlo, Iryna Ilkiv, Roman Lesyk, and Alexander Sklyarov
Walter de Gruyter GmbH
Abstract The use of nonsteroidal anti-inflammatory drugs (NSAIDs) in combination with being physiologically stressed often occurs in in the course of different pathologies. This situation may result in the alteration of digestive system functioning. The effect of stress brings about changes in the activity of nitric oxide synthase (NOS), arginase, cyclooxygenase (COX) and lipid peroxidation, whereas the use of NSAIDs interrupts the multiple functions of the cell via the inhibition of prostaglandins (PGs) synthesis. Taking into account that NOS and COX-systems are connected in their regulation, the aim of the study was to determine the role played by NOS and lipid peroxidation under conditions of the combined action of NSAIDs and stress. In our study, male rats were used. The NSAIDs (naproxen - a non-selective COX inhibitor, celecoxib - a selective COX-2 blocker, and the compound 2A5DHT (which is the active substance of dual COX, and the lipoxygenase (LOX) inhibitor, darbufelone) were all administered at a dose 10 mg/kg, prior to water restraint stress (WRS). WRS brought about an increase of inducible NOS (iNOS) activity in the intestinal mucosal and muscular membranes, as well as in the pancreas. Because of this, constitutive NOS izoform (cNOS) and arginase activities decreased. Moreover, the MDA concentration increased, indicating the development of oxidative stress. In our work, pretreatment with naproxen, as in the WRS model, engendered a decrease in iNOS activity. What is more, administration of Celecoxib did not change iNOS activity, as compared to WRS alone, and it showed a tendency to reduce lipid peroxidation. In addition, 2A5DHT prior WRS brought about a decrease of iNOS activity, with the subsequent rise of cNOS activity. Of note, MDA concentration decreased in all studied organs, indicating the reduction of lipid peroxidation under the action of the darbufelone active substance.

Effects of conventional and hydrogen sulfide-releasing non-steroidal anti-inflammatory drugs in rats with stress-induced and epinephrine-induced gastric damage
Iryna Fomenko, Alexander Sklyarov, Tetyana Bondarchuk, Lilya Biletska, Natalia Panasyuk, and John L. Wallace
Informa UK Limited
Abstract Mechanisms of gastric defence under conditions of combined influence of acute stress and non-steroidal anti-inflammatory drugs (NSAIDs) are still poorly studied. The aim of this study was to explore the effects of different types of NSAIDs (naproxen, celecoxib and ATB-346) in producing experimental gastric lesions (induced by water-restraint stress (WRS) or by epinephrine (EPN) injection) and to determine the role of lipid peroxidation and the nitric oxide (NO) system in the pathogenesis of the damage. Male rats were used (eight per group) in this work. The NSAIDs were all administered at a dose 10 mg kg−1 30 min prior to WRS or EPN injection. Administration of naproxen to the control rats caused development of gastric lesions, whereas administration of a hydrogen sulfide (H2S)-releasing NSAID (ATB-346) or a selective cyclooxygenase-2 inhibitor (celecoxib) did not cause gastric damage. In contrast, lipid peroxidation processes were enhanced in all groups as was the activity of NO synthase (NOS). Pretreatment with naproxen in the WRS model caused an increase in severity of damage and a decrease in NOS activity. ATB-346 displayed beneficial effects, manifested by a decrease in the area of gastric damage, but parameters of lipid peroxidation and the NOS system did not differ substantially from those in the group treated with naproxen. Administration of different NSAIDs under conditions of EPN-induced gastric damage resulted in the decrease in NOS activity and lipid peroxidation. None of the tested NSAIDs exacerbated EPN-induced gastric mucosal injury; indeed, they all reduced the extent of damage.

[Parameters of NO synthase system of gastric mucosa in rats under stress conditions and inhibition of cyclooxygenase].
IS Fomenko, , TI Bondarchuk, LP Bilets'ka, NB Panasiuk, OIa Skliarov, , , , and
National Academy of Sciences of Ukraine (Co. LTD Ukrinformnauka)
In experiments on rats with modeled water-restrained stress, the influence of nonsteroidal anti-inflammatory drugs of different genesis on morphological status of gastric mucosa and changes of NO-synthase system parameters have been studied Administration of nonselective cyclooxygenese inhibitor naproxen in the water-restrained stress model in rats potentiated the increase of severity of damage of gastric mucosa. At the same time, the activity of both inducible and constitutive isoforms ofNO-sythase decreased. The parameters of lipoperoxidation remained at the level observed during water-restrained stress. It was shown the advantages of the use of H2S-releasinfg nonsteroidal anti-inflammatory drug ATB-346, which are associated with its cytoprotective effect of the drug manifested by a decreased total area of gastric damage. However, parameters of lipoperoxidation and NO-syntase system did not differ substantially from those in the group treated with napoxen, indicating the prevalence of parent molecule (naproxen) in regulation of function of NO-system Administration of dual COX/LOX inhibitor, the compound 2A5DHT, caused a decrease of gastric damage as compared to the effect ofnaproxen. The activity of iNOS remained much higher than under condition of the naproxen action.

Dopamine receptor type 1 of Caenorhabditis elegans expressing in mechanosensory neurons
V. V. Stadnyk, A. Regosh, C. Y. Mayor, I. S. Fomenko, T. I. Bondarchuk, and O. Y. Sklyarov
Institute of Molecular Biology and Genetics (NAS Ukraine)
Until now the results on profiling dopamine receptors in C. elegans have been incomplete and fragmentary. The aim of this study was to investigate the expression profile of dop-1 gene in C. elegans using 3 kb promoter with 3'-end locating before ATG of dop-1gene. Methods. The strain of C. elegans with mutant unc-119 gene was used. To check a pattern of the dop-1 expression, the promoter of this gene was amplified using PCR. The animals were co-bombarded with plasmid pPD95.77 dop-1::GFP and reporter construct containing unc-119 gene. Results. Using GFP as a reporter protein, we built a whole picture of expression of dopamine receptor type 1 in C. elegans and found that this protein could be detected only in mechanosensory neurons such as PLM, PVQR, PVQL, ALNR, ALNL, DVAR, DVC.

Dual acting COX/LOX nonsteroidal anti-inflammatory drugs versus traditional COX-2 inhibitors

The effect of COX-2 inhibitor celecoxib and proton pump blocker lansoprazole on lipoperoxidation processes in heart tissue and gastric mucosa of streptozotocin-induced diabetic in rats

Combined effects of neurotransmitters upon secretory function of the rat stomach at different functional states of the gastric mucosa
A. Yu. Sklyarov, A. M. Maslyanko, and T. I. Bondarchuk
Springer Science and Business Media LLC

RECENT SCHOLAR PUBLICATIONS

[PDF] з домену dmed.org.ua Гідроген сульфід: біологічні та патохімічні ефекти
ТІБІС ФОМЕНКО
ЕКСПЕРИМЕНТАЛЬНА ТА КЛІНІЧНА ФІЗІОЛОГІЯ І БІОХІМІЯ “EXPERIMENTAL AND 2024

Fomenko Iryna Особливості застосування інтерактивних форм навчання при вивченні біохімії у Львівському національному медичному університеті імені Данила Галицького за умов
ТІБІС ФОМЕНКО
ЕКСПЕРИМЕНТАЛЬНА ТА КЛІНІЧНА ФІЗІОЛОГІЯ І БІОХІМІЯ “EXPERIMENTAL AND 2022

Anti-inflammatory hydrogen sulfide-releasing agents with reduced gastro-and enterotoxicity on the stress model in rats.
I Fomenko, I Lozynska, T Bondarchuk, N Denysenko, R Lesyk, A Sklyarov
Minerva Biotechnology & Biomolecular Research 33 (2) 2021

Minerva Biotechnology and Biomolecular Research 2021 June; 33 (2): 117-24
I FOMENKO, I LOZYNSKA, T BONDARCHUK, N DENYSENKO, R LESYK, ...
Minerva 33 2021

ІТРОЗО-ОКСИДАТИВНІ ПРОЦЕСИ В ОРГАНАХ ТРАВНОЇ СИСТЕМИ ПРИ ДІЇ L-АРГІНІНУ ТА АМІНОГУАНІДИНУ ЗА УМОВ ЕКСПЕРИМЕНТАЛЬНОГО ЦУКРОВОГО ДІАБЕТУ
НВД О.Я. Скляров О.І. Децик , О.Б. Панчишин , І.С. Фоменко , Т.І. Бондарчук ...
Art of Medicine 18 (2), 44-49 2021

Цитопротекторний вплив NO, Н2S та системи циклооксигеназа/простагландини в слизовій оболонці товстої кишки щурів при виразковому коліті
ОЯС ІС Фоменко, ТІ Бондарчук, АС Юет
Physiological Journal/Fiziologichnyi Zhurnal 66 (5) 2020

Дослідження впливу донорів гідрогену сульфіду на ступінь оксидативного стресу при експериментальному гострому панкреатиті у щурів.
ТІБІС ФОМЕНКО
Вісник біології та медицини 155, 98-102 2020

Changes of nitric oxide system and lipid peroxidation parameters in the digestive system of rats under conditions of acute stress, and use of nonsteroidal anti-inflammatory drugs
I Fomenko, T Bondarchuk, V Emelyanenko, N Denysenko, S Pavlo, I Ilkiv, ...
Current Issues in Pharmacy and Medical Sciences 28 (1), 37-41 2015

Peculiarities of Effects of Nonsteroidal Anti-inflammatory Drugs on NO-synthase System in Gastric Mucosa of Rats under Stress Conditions
IS Fomenko, TI Bondarchuk, LP Biletska, NB Panasyuk, OY Sklyarov
International Journal of Physiology and Pathophysiology 6 (4) 2015

Effects of conventional and hydrogen sulfide-releasing non-steroidal anti-inflammatory drugs in rats with stress-induced and epinephrine-induced gastric damage
I Fomenko, A Sklyarov, T Bondarchuk, L Biletska, N Panasyuk, JL Wallace
Stress 17 (6), 528-537 2014

Action of Different NSAIDs on Parameters of NO-Synthase System in Pancreas of Rats Under Condition of Water Immersion Restrain Stress
TI Bondarchuk, IS Fomenko, LP Biletska, NB Panasyuk, AY Sklyarov
DIGESTIVE DISEASES AND SCIENCES 59 (8), 1649-1649 2014

Pecularities of the Changes of NO-Synthase/Arginase System in Digestive Organs Under The Conditions of Stress and COX-1/COX-2, COX-2/5-LOX Blockage
IS Fomenko, NB Panasyuk, II Ilkiv, TI Bondarchuk, PA Sklyarov, ...
DIGESTIVE DISEASES AND SCIENCES 59 (8), 1654-1654 2014

Вплив вітамінів С та Е на процеси ліпопероксидації та активність NO-синтазної системи в підшлунковій залозі щурів на тлі адреналін-індукованого стресу
TI Bondarchuk
Буковинський медичний вісник 18 (2 (70)) 2014

Parameters of NO synthase system of gastric mucosa in rats under stress conditions and inhibition of cyclooxygenase
IS Fomenko, TI Bondarchuk, LP Bilets' ka, NB Panasiuk, OI Skliarov
Fiziolohichnyi zhurnal (Kiev, Ukraine: 1994) 60 (2), 51-56 2014

INFLUENCE OF VITAMINS C AND E ON LIPID PEROXIDATION PROCESSES AND THE ACTIVITY OF NO-SYNTHASE SYSTEM IN PANCREAS OF RATS WITH THE EPINEPHRINE-INDUCED STRESS
TI Bondarchuk
ДОСЯГНЕННЯ ЦІЛЬОВОГО РІВНЯ АРТЕРІАЛЬНОГО ТИСКУ У ХВОРИХ НА АРТЕРІАЛЬНУ 2014

Ген дофамінового рецептора першого типу експресується в механосенсорних нейронах Caenorhabditis elegans
ВВ Стадник, А Регош, ХЯ Майор, ІС Фоменко, ТІ Бондарчук, ...
Biopolymers & Cell 28 (1), 75 2012

Dopamine receptor type 1 of Caenorhabditis elegans expressing in mechanosensory neurons
VV Stadnyk, A Regosh, CY Mayor, IS Fomenko, TI Bondarchuk, ...
Вiopolymers and Cell 2012

Dual acting COX/LOX nonsteroidal anti-inflammatory drugs versus traditional COX-2 inhibitors
I Fomenko, T Bondarchuk, O Sklyarov
Pharmacia 23 (3), 197-202 2010

The effect of COX-2 inhibitor celecoxib and proton pump blockerlansoprazole on lipoperoxidation processes in heart tissueand gastric mucosa of streptozotocin-induced diabetic
I Fomenko, T Bondarchuk, O Sklyarov, K Swierzko, J Solski
Current Issues in Pharmacy and Medical Sciences 22 (3), 31-35 2009

Морфо-функціональні, метаболічні процеси та кровотік у слизовій оболонці шлунка при різних його функціональних станах
ТІ Бондарчук
ступеня канд. мед наук: 14.03. 03/ТІ Бондарчук 2002

MOST CITED SCHOLAR PUBLICATIONS

Changes of nitric oxide system and lipid peroxidation parameters in the digestive system of rats under conditions of acute stress, and use of nonsteroidal anti-inflammatory drugs
I Fomenko, T Bondarchuk, V Emelyanenko, N Denysenko, S Pavlo, I Ilkiv, ...
Current Issues in Pharmacy and Medical Sciences 28 (1), 37-41 2015
Citations: 15

Dual acting COX/LOX nonsteroidal anti-inflammatory drugs versus traditional COX-2 inhibitors
I Fomenko, T Bondarchuk, O Sklyarov
Pharmacia 23 (3), 197-202 2010
Citations: 2

Combined effects of neurotransmitters upon secretory function of the rat stomach at different functional states of the gastric mucosa
AY Sklyarov, AM Maslyanko, TI Bondarchuk
Neurophysiology 31 (1), 56-58 1999
Citations: 1