Clare Elizabeth O'Lone

@ecu.edu.au

Australian Research Council Centre of Excellence for Innovations in Peptide and Protein Science (CIPPS), School of Science
ARC Centre of Excellence for Innovations in Peptide and Protein Science (CIPPS), Edith Cowan University

Clare Elizabeth O'Lone


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https://researchid.co/clare.olone

RESEARCH, TEACHING, or OTHER INTERESTS

Agricultural and Biological Sciences, Plant Science, Biochemistry, Genetics and Molecular Biology
6

Scopus Publications

438

Scholar Citations

7

Scholar h-index

6

Scholar i10-index

Scopus Publications

  • Solvent-dependent proteome profiling in edible mushrooms (Hypsizygus marmoreus and Agaricus bisporus)
    Tanvir Ahmed, Clare O'Lone, Angéla Juhász, Utpal Bose, Kenneth U. Agbo, et al.
    Journal of Food Composition and Analysis, 2026
  • Advancing Sustainable Malting Practices: Aquaporins as Potential Breeding Targets for Improved Water Uptake during Controlled Germination of Barley (Hordeum vulgare L.)
    Clare E. O’Lone, Angéla Juhász, Mitchell Nye-Wood, David Moody, Hugh Dunn, et al.
    Journal of Agricultural and Food Chemistry, 2024
    The conversion of raw barley (Hordeum vulgare L.) to malt requires a process of controlled germination, where the grain is submerged in water to raise the moisture content to >40%. The transmembrane proteins, aquaporins, influence water uptake during the initial stage of controlled germination, yet little is known of their involvement in malting. With the current focus on sustainability, understanding the mechanisms of water uptake and usage during the initial stages of malting has become vital in improving efficient malting practices. In this study, we used quantitative proteomics analysis of two malting barley genotypes demonstrating differing water-uptake phenotypes in the initial stages of malting. Our study quantified 19 transmembrane proteins from nine families, including seven distinct aquaporin isoforms, including the plasma intrinsic proteins (PIPs) PIP1;1, PIP2;1, and PIP2;4 and the tonoplast intrinsic proteins (TIPs) TIP1;1, TIP2;3, TIP3;1, and TIP3;2. Our findings suggest that the presence of TIP1;1, TIP3;1, and TIP3;2 in the mature barley grain proteome is essential for facilitating water uptake, influencing cell turgor and the formation of large central lytic vacuoles aiding storage reserve hydrolysis and endosperm modification efficiency. This study proposes that TIP3s mediate water uptake in malting barley grain, offering potential breeding targets for improving sustainable malting practices.
  • Proteomic exploration reveals a metabolic rerouting due to low oxygen during controlled germination of malting barley (Hordeum vulgare L.)
    Clare E. O'Lone, Angéla Juhász, Mitchell Nye-Wood, Hugh Dunn, David Moody, et al.
    Frontiers in Plant Science, 2023
    Barley (Hordeum vulgare L.) is used in malt production for brewing applications. Barley malting involves a process of controlled germination that modifies the grain by activating enzymes to solubilize starch and proteins for brewing. Initially, the grain is submerged in water to raise grain moisture, requiring large volumes of water. Achieving grain modification at reduced moisture levels can contribute to the sustainability of malting practices. This study combined proteomics, bioinformatics, and biochemical phenotypic analysis of two malting barley genotypes with observed differences in water uptake and modification efficiency. We sought to reveal the molecular mechanisms at play during controlled germination and explore the roles of protein groups at 24 h intervals across the first 72 h. Overall, 3,485 protein groups were identified with 793 significant differentially abundant (DAP) within and between genotypes, involved in various biological processes, including protein synthesis, carbohydrate metabolism, and hydrolysis. Functional integration into metabolic pathways, such as glycolysis, pyruvate, starch and sucrose metabolism, revealed a metabolic rerouting due to low oxygen enforced by submergence during controlled germination. This SWATH-MS study provides a comprehensive proteome reference, delivering new insights into the molecular mechanisms underlying the impacts of low oxygen during controlled germination. It is concluded that continued efficient modification of malting barley subjected to submergence is largely due to the capacity to reroute energy to maintain vital processes, particularly protein synthesis.
  • Application of Mass Spectrometry-Based Proteomics to Barley Research
    Mahya Bahmani, Clare E. O’Lone, Angéla Juhász, Mitchell Nye-Wood, Hugh Dunn, et al.
    Journal of Agricultural and Food Chemistry, 2021
    Barley (Hordeum vulgare) is the fourth most cultivated crop in the world in terms of production volume, and it is also the most important raw material of the malting and brewing industries. Barley belongs to the grass (Poaceae) family and plays an important role in food security and food safety for both humans and livestock. With the global population set to reach 9.7 billion by 2050, but with less available and/or suitable land for agriculture, the use of biotechnology tools in breeding programs are of considerable importance in the quest to meet the growing food gap. Proteomics as a member of the “omics” technologies has become popular for the investigation of proteins in cereal crops and particularly barley and its related products such as malt and beer. This technology has been applied to study how proteins in barley respond to adverse environmental conditions including abiotic and/or biotic stresses, how they are impacted during food processing including malting and brewing, and the presence of proteins implicated in celiac disease. Moreover, proteomics can be used in the future to inform breeding programs that aim to enhance the nutritional value and broaden the application of this crop in new food and beverage products. Mass spectrometry analysis is a valuable tool that, along with genomics and transcriptomics, can inform plant breeding strategies that aim to produce superior barley varieties. In this review, recent studies employing both qualitative and quantitative mass spectrometry approaches are explored with a focus on their application in cultivation, manufacturing, processing, quality, and the safety of barley and its related products.
  • Regeneration selection improves transformation efficiency in narrow-leaf lupin
    Susan J. Barker, Ping Si, Leon Hodgson, Margo Ferguson-Hunt, Yuphin Khentry, et al.
    Plant Cell Tissue and Organ Culture, 2016
  • The first gene-based map of Lupinus angustifolius L.-location of domestication genes and conserved synteny with Medicago truncatula
    Matthew N. Nelson, Huyen T. T. Phan, Simon R. Ellwood, Paula M. Moolhuijzen, James Hane, et al.
    Theoretical and Applied Genetics, 2006

RECENT SCHOLAR PUBLICATIONS

  • Solvent-dependent proteome profiling in edible mushrooms (Hypsizygus marmoreus and Agaricus bisporus)
    T Ahmed, C O'Lone, A Juhász, U Bose, KU Agbo, NS Terefe, ML Colgrave
    Journal of Food Composition and Analysis, 109074 , 2026
    2026.0
  • Advancing sustainable malting practices: Aquaporins as potential breeding targets for improved water uptake during controlled germination of barley (hordeum vulgare l.)
    CE O’Lone, A Juhász, M Nye-Wood, D Moody, H Dunn, JP Ral, ...
    Journal of Agricultural and Food Chemistry 72 (17), 10149-10161 , 2024
    2024.0
    Citations: 8
  • Proteomic understanding of grain germination to improve the selection of barley varieties with internationally competitive malting quality characteristics
    CE O'Lone
    Edith Cowan University , 2024
    2024.0
  • Proteomic exploration reveals a metabolic rerouting due to low oxygen during controlled germination of malting barley (Hordeum vulgare L.)
    CE O'Lone, A Juhasz, MG Nye-Wood, H Dunn, D Moody, JP Ral, ...
    Frontiers in Plant Science 14, 1305381 , 2023
    2023.0
    Citations: 5
  • Application of mass spectrometry-based proteomics to barley research
    M Bahmani, CE O’Lone, A Juhasz, M Nye-Wood, H Dunn, IB Edwards, ...
    Journal of Agricultural and Food Chemistry 69 (31), 8591-8609 , 2021
    2021.0
    Citations: 43
  • Regeneration selection improves transformation efficiency in narrow-leaf lupin
    SJ Barker, P Si, L Hodgson, M Ferguson-Hunt, Y Khentry, ...
    Plant Cell, Tissue and Organ Culture (PCTOC) 126 (2), 219-228 , 2016
    2016.0
    Citations: 18
  • New evidence of ancestral polyploidy in the Genistoid legume Lupinus angustifolius L. (narrow-leafed lupin)
    M Kroc, G Koczyk, W Święcicki, A Kilian, MN Nelson
    Theoretical and Applied Genetics 127 (5), 1237-1249 , 2014
    2014.0
    Citations: 86
  • A new method for producing allohexaploid Brassica through unreduced gametes
    AS Mason, G Yan, WA Cowling, MN Nelson
    Euphytica 186 (2), 277-287 , 2012
    2012.0
    Citations: 42
  • Genotypic effects on the frequency of homoeologous and homologous recombination in Brassica napus × B. carinata hybrids
    AS Mason, MN Nelson, MC Castello, G Yan, WA Cowling
    Theoretical and Applied Genetics 122 (3), 543-553 , 2011
    2011.0
    Citations: 55
  • Development, characterization and transferability of microsatellite markers for Cullen australasicum (Leguminosae)
    L Kroiss, M Moody, SJ Barker, M Byrne, MH Ryan
    Conservation Genetics 10 (6), 1803-1805 , 2009
    2009.0
    Citations: 7
  • Molecular characterisation of microspore-derived progeny from the interspecific F1 of Brassica napus× B. carinata
    MN Nelson, A Mason, MC Castello, CE O'Lone, G Yan, S Chen, ...
    Molecular characterisation of microspore-derived progeny from the … , 2007
    2007.0
    Citations: 1
  • The first gene-based map of Lupinus angustifolius L.-location of domestication genes and conserved synteny with Medicago truncatula
    MN Nelson, HTT Phan, SR Ellwood, PM Moolhuijzen, J Hane, A Williams, ...
    Theoretical and Applied Genetics 113 (2), 225-238 , 2006
    2006.0
    Citations: 173
  • Solvent-dependent proteome profiling in edible mushrooms (Hypsizygus marmoreus and Agaricus bisporus)
    ML Colgrave, T Ahmed, C O'Lone, A Juhasz, U Bose, KU Agbo, NS Terefe
    Available at SSRN 5742976 , 0

MOST CITED SCHOLAR PUBLICATIONS

  • The first gene-based map of Lupinus angustifolius L.-location of domestication genes and conserved synteny with Medicago truncatula
    MN Nelson, HTT Phan, SR Ellwood, PM Moolhuijzen, J Hane, A Williams, ...
    Theoretical and Applied Genetics 113 (2), 225-238 , 2006
    2006.0
    Citations: 173
  • New evidence of ancestral polyploidy in the Genistoid legume Lupinus angustifolius L. (narrow-leafed lupin)
    M Kroc, G Koczyk, W Święcicki, A Kilian, MN Nelson
    Theoretical and Applied Genetics 127 (5), 1237-1249 , 2014
    2014.0
    Citations: 86
  • Genotypic effects on the frequency of homoeologous and homologous recombination in Brassica napus × B. carinata hybrids
    AS Mason, MN Nelson, MC Castello, G Yan, WA Cowling
    Theoretical and Applied Genetics 122 (3), 543-553 , 2011
    2011.0
    Citations: 55
  • Application of mass spectrometry-based proteomics to barley research
    M Bahmani, CE O’Lone, A Juhasz, M Nye-Wood, H Dunn, IB Edwards, ...
    Journal of Agricultural and Food Chemistry 69 (31), 8591-8609 , 2021
    2021.0
    Citations: 43
  • A new method for producing allohexaploid Brassica through unreduced gametes
    AS Mason, G Yan, WA Cowling, MN Nelson
    Euphytica 186 (2), 277-287 , 2012
    2012.0
    Citations: 42
  • Regeneration selection improves transformation efficiency in narrow-leaf lupin
    SJ Barker, P Si, L Hodgson, M Ferguson-Hunt, Y Khentry, ...
    Plant Cell, Tissue and Organ Culture (PCTOC) 126 (2), 219-228 , 2016
    2016.0
    Citations: 18
  • Advancing sustainable malting practices: Aquaporins as potential breeding targets for improved water uptake during controlled germination of barley (hordeum vulgare l.)
    CE O’Lone, A Juhász, M Nye-Wood, D Moody, H Dunn, JP Ral, ...
    Journal of Agricultural and Food Chemistry 72 (17), 10149-10161 , 2024
    2024.0
    Citations: 8
  • Development, characterization and transferability of microsatellite markers for Cullen australasicum (Leguminosae)
    L Kroiss, M Moody, SJ Barker, M Byrne, MH Ryan
    Conservation Genetics 10 (6), 1803-1805 , 2009
    2009.0
    Citations: 7
  • Proteomic exploration reveals a metabolic rerouting due to low oxygen during controlled germination of malting barley (Hordeum vulgare L.)
    CE O'Lone, A Juhasz, MG Nye-Wood, H Dunn, D Moody, JP Ral, ...
    Frontiers in Plant Science 14, 1305381 , 2023
    2023.0
    Citations: 5
  • Molecular characterisation of microspore-derived progeny from the interspecific F1 of Brassica napus× B. carinata
    MN Nelson, A Mason, MC Castello, CE O'Lone, G Yan, S Chen, ...
    Molecular characterisation of microspore-derived progeny from the … , 2007
    2007.0
    Citations: 1
  • Solvent-dependent proteome profiling in edible mushrooms (Hypsizygus marmoreus and Agaricus bisporus)
    T Ahmed, C O'Lone, A Juhász, U Bose, KU Agbo, NS Terefe, ML Colgrave
    Journal of Food Composition and Analysis, 109074 , 2026
    2026.0
  • Proteomic understanding of grain germination to improve the selection of barley varieties with internationally competitive malting quality characteristics
    CE O'Lone
    Edith Cowan University , 2024
    2024.0
  • Solvent-dependent proteome profiling in edible mushrooms (Hypsizygus marmoreus and Agaricus bisporus)
    ML Colgrave, T Ahmed, C O'Lone, A Juhasz, U Bose, KU Agbo, NS Terefe
    Available at SSRN 5742976 , 0