8-Oxo-2′-Deoxyguanosine: Oxidative Stress Control N. V. Marmiy, D. S. Esipov Russian Journal of Bioorganic Chemistry, 2025 8-Oxo-2′-deoxyguanosine is a well-known marker of oxidative stress. Research over the past decade suggests that this compound is probably not a byproduct of oxidative DNA damage, but an important bioregulator driving the cellular response to stress. This review collected and analyzed data on the participation of 8-oxo-2′-deoxyguanosine in the processes of mutagenesis, DNA repair, and regulation of gene expression, inflammatory responses, adaptive stress response, apoptosis, and cellular transformation. Particular attention is paid to the potential of 8-oxo-2′-deoxyguanosine as a therapeutic agent for the treatment of inflammatory, autoimmune, degenerative, and oncological diseases, as well as of traumatic and toxic injuries.
Effect of Deletions of the Genes Encoding Pho3p and Bgl2p on Polyphosphate Level, Stress Adaptation, and Attachments of These Proteins to Saccharomyces cerevisiae Cell Wall Tatyana S. Kalebina, Ekaterina V. Kulakovskaya, Valentina V. Rekstina, Ludmila V. Trilisenko, Rustam H. Ziganshin, Natalia V. Marmiy, Dmitriy S. Esipov, Tatiana V. Kulakovskaya Biochemistry Moscow, 2023 Inorganic polyphosphates (polyP), according to literature data, are involved in the regulatory processes of molecular complex of the Saccharomyces cerevisiae cell wall (CW). The aim of the work was to reveal relationship between polyP, acid phosphatase Pho3p, and the major CW protein, glucanosyltransglycosylase Bgl2p, which is the main glucan-remodelling enzyme with amyloid properties. It has been shown that the yeast cells with deletion of the PHO3 gene contain more high molecular alkali-soluble polyP and are also more resistant to exposure to alkali and manganese ions compared to the wild type strain. This suggests that Pho3p is responsible for hydrolysis of the high molecular polyP on the surface of yeast cells, and these polyP belong to the stress resistance factors. The S. cerevisiae strain with deletion of the BGL2 gene is similar to the Δpho3 strain both in the level of high molecular alkali-soluble polyP and in the increased resistance to alkali and manganese. Comparative analysis of the CW proteins demonstrated correlation between the extractability of the acid phosphatase and Bgl2p, and also revealed a change in the mode of Bgl2p attachment to the CW of the strain lacking Pho3p. It has been suggested that Bgl2p and Pho3p are able to form a metabolon or its parts that connects biogenesis of the main structural polymer of the CW, glucan, and catabolism of an important regulatory polymer, polyphosphates.
Pseudo-minimal surfaces of revolution , Mikhail S. Bukhtyak, Dmitrii E. Yesipov, and Vestnik Tomskogo Gosudarstvennogo Universiteta Matematika I Mekhanika, 2022 This paper is a follow-up to the first author’s series of works about shape modeling of orthotropic elastic material that takes the equilibrium form inside the area with the specified boundaries. M.S. Bukhtyak, in a number of his publications of 20162020, proposed an approach to the model building based on the application of surfaces with a constant ratio of principal curvatures. These surfaces are named pseudo-minimal surfaces. The theorem of existence has been demonstrated and the finitely-element model has been built. The condition distinguishing the class of pseudo-minimal surfaces, as applied to ruled surfaces, is either satisfied identically (trivial subclasses) or is satisfied along a family of lines. The corresponding classes of ruled surfaces have been comprehensively characterized geometrically. A partial differential equation that defines (in the local sense) the class of pseudo-minimal surfaces is very complex for analysis, which makes it relevant to consider approximate solutions. The current paper considers the pseudo-minimal surfaces of revolution. Generation of the approximate solutions is complicated by the tendency of the formal Taylor polynomial to diverge. However, the approximate solutions (of course, not ideal) have been generated. Contribution of the authors: the authors contributed equally to this article. The authors declare no conflicts of interests.
Mitochondria-targeted 1,4-naphthoquinone (SkQN) is a powerful prooxidant and cytotoxic agent Tatyana N. Goleva, Konstantin G. Lyamzaev, Anton G. Rogov, Ljudmila S. Khailova, Khoren K. Epremyan, Galina P. Shumakovich, Lidia V. Domnina, Olga Yu. Ivanova, Natalia V. Marmiy, Tatiana V. Zinevich, Dmitry S. Esipov, Renata A. Zvyagilskaya, Vladimir P. Skulachev, Boris V. Chernyak Biochimica Et Biophysica Acta Bioenergetics, 2020
Polyanionic carboxyethyl peptide nucleic acids (ce-PNAs): Synthesis and DNA binding Yuliya Kirillova, Nataliya Boyarskaya, Andrey Dezhenkov, Mariya Tankevich, Ivan Prokhorov, Anna Varizhuk, Sergei Eremin, Dmitry Esipov, Igor Smirnov, Galina Pozmogova Plos One, 2015 New polyanionic modifications of polyamide nucleic acid mimics were obtained. Thymine decamers were synthesized from respective chiral α- and γ-monomers, and their enantiomeric purity was assessed. Here, we present the decamer synthesis, purification and characterization by MALDI-TOF mass spectrometry and an investigation of the hybridization properties of the decamers. We show that the modified γ-S-carboxyethyl-T10 PNA forms a stable triplex with polyadenine DNA.
Biological role of 8-oxo-2'-deoxyguanosine N. V. Marmiy, D. S. Esipov Moscow University Biological Sciences Bulletin, 2015 The review presents currently available data on the biological role of 8-oxo-2’-deoxyguanosine. This compound for a long time has been used successfully as a biomarker of oxidative stress and diseases associated with it. However, in recent years, an increasing number of publications has appeared reporting that biological role of 8-oxo-dG is not only a mutagenic one; it also is involved in the regulation of gene expression, in some processes of DNA repair, in the control of inflammatory and autoimmune reactions, and in the activation of antioxidant systems. Probably, 8-oxo-2’-deoxyguanosine could prospectively be used as a drug.
SkBQ - Prooxidant addressed to mitochondria M. Y. Vyssokikh, B. V. Chernyak, L. V. Domnina, D. S. Esipov, O. Y. Ivanova, G. A. Korshunova, R. A. Symonyan, M. V. Skulachev, T. V. Zinevich, V. P. Skulachev Biochemistry Moscow, 2013 Oxidative stress and mitochondrial dysfunction are the key links in the chain of development of pathologies associated with the violation of cellular energy metabolism. Development of mitochondria-addressed compounds highly specific for chemical processes is one of the most promising ways to develop approaches to the treatment of inherited and age-related diseases with mitochondrial etiology. Correlation of structure and chemical activity of the test compounds from a class of lipophilic cations revealed the key role of substituents in the aromatic ring of 1,4-benzoquinones in the manifestation of high antioxidant properties. In this work, it is shown that a synthesized benzoquinone derivative conjugated in position 6 with membrane-penetrating cation of decyltriphenylphosphonium and with substituents at position 2, 3, and 5 (SkBQ) has much lower antioxidant and significantly higher prooxidant activity in comparison with similar derivatives of plasto- and toluquinone SkQ1 and SkQT1 in experiments on isolated mitochondria. At the same time, SkBQ, like SkQ1 and SkQT1, can be reduced by the respiratory chain in the center i of complex III and decrease the mitochondrial membrane potential. In cell cultures of human fibroblasts, it was revealed that SkBQ does not protect cells from apoptosis induced by hydrogen peroxide. Under the same conditions, SkQ1 and SkQT1 exhibit a powerful protective effect. Thus, SkBQ can be seen as a mitochondria-addressed prooxidant. The possibility of using SkBQ as an anticancer drug for the treatment of cancers such as prostate cancer whose cells are sensitive to mitochondrial reactive oxygen species is discussed.
Potential geroprotector properties of the extract of reindeer antler powder in experiments on cultured cells Advances in Gerontology Uspekhi Gerontologii Rossiskai X0361 A Akademii X0361 A Nauk Gerontologicheskoe Obshchestvo, 2008
8-oxo-2'-deoxyguanosine accumulation in DNA from "stationary phase aging" cultured cells Advances in Gerontology Uspekhi Gerontologii Rossiskai X0361 A Akademii X0361 A Nauk Gerontologicheskoe Obshchestvo, 2008
Determination of oligonucleotide molecular masses by MALDI mass spectrometry Bioorganicheskaya Khimiya, 2005
The synthesis of phosphoceramide azidothymidine and phosphoceramide didehydrodeoxythymidine Russian Journal of Bioorganic Chemistry, 1997
The Synthesis of Phosphoceramide Azidothymidine and Phosphoceramide Didehydrodeoxythymidine Bioorganicheskaya Khimiya, 1997
Preparation of Ceramide Phosphothymidine, a Model Compound for the Sphingophosphooligonucleotide Synthesis Bioorganicheskaya Khimiya, 1996
Mutants of Human Tumor Necrosis Factor: Preparation and Properties Bioorganicheskaya Khimiya, 1996
Preparation of ceramide phosphothymidine, a model compound for the sphingophosphooligonucleotide synthesis Russian Journal of Bioorganic Chemistry, 1996
Mutants of human tumor necrosis factor: Preparation and properties Russian Journal of Bioorganic Chemistry, 1996
Human tumor necrosis factor mutants: preparation and some properties Bioorganicheskaia Khimiia, 1996
Study of resistance of the human immunodeficiency virus to azidothymidine. II. Kinetic characteristics of 'AZT-resistant' mutant forms of reverse transcriptase Biokhimiya, 1996
Study on the resistance of human immunodeficiency virus to azidothymidine. II. Kinetic characteristics of "AZT-resistant mutants" of reverse transcriptase Biochemistry Moscow, 1996
Selectively cleaved synthetic oligodeoxyribonucleotides for reverse immobilization of DNA Bioorganicheskaia Khimiia, 1994
Studies of the resistance of the human immunodeficiency virus to azidothymidine. I. Kinetic studies of the interaction between reverse transcriptase and a series of its mutant forms with substrates and azidothymidine-5'-trisphosphate Biokhimiya, 1994
Hybrid human lymphokine genes. I.Design of recombinant plasmids coding hybrids of immune interferon and human tumor necrosis factor Bioorganicheskaia Khimiia, 1991
Enzymatic ligation of DNA fragments containing phosphoamide modification of the internucleotide bonds Bioorganicheskaia Khimiia, 1991
Construction and properties of fusion proteins between human interferon-gamma and human tumour necrosis factors alpha and beta. Biomedical Science, 1991
Solid phase ligation of synthetic DNA fragments Bioorganicheskaia Khimiia, 1990
Synthesis of 5'-phosphorylated oligodeoxyribonucleotides by the H-phosphonate method Bioorganicheskaia Khimiia, 1989
