Anne-Sophie Hascoet
@mpaveterinary.com
MPA Veterinary Medicines and Additives
RESEARCH, TEACHING, or OTHER INTERESTS
Veterinary, Food Animals, Food Science
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
Alfons Eduard Guerrero-Navarro, Abel Guillermo Ríos-Castillo, Carolina Ripolles-Avila, Anna Zamora, Anne-Sophie Hascoët, Xavier Felipe, Manuel Castillo, and José Juan Rodríguez-Jerez
Elsevier BV
Anne-Sophie Hascoët, Carolina Ripolles-Avila, Brayan R. H. Cervantes-Huamán, and José Juan Rodríguez-Jerez
MDPI AG
Listeria monocytogenes continues to be one of the most important public health challenges for the meat sector. Many attempts have been made to establish the most efficient cleaning and disinfection protocols, but there is still the need for the sector to develop plans with different lines of action. In this regard, an interesting strategy could be based on the control of this type of foodborne pathogen through the resident microbiota naturally established on the surfaces. A potential inhibitor, Bacillus safensis, was found in a previous study that screened the interaction between the resident microbiota and L. monocytogenes in an Iberian pig processing plant. The aim of the present study was to evaluate the effect of preformed biofilms of Bacillus safensis on the adhesion and implantation of 22 strains of L. monocytogenes. Mature preformed B. safensis biofilms can inhibit adhesion and the biofilm formation of multiple L. monocytogenes strains, eliminating the pathogen by a currently unidentified mechanism. Due to the non-enterotoxigenic properties of B. safensis, its presence on certain meat industry surfaces should be favored and it could represent a new way to fight against the persistence of L. monocytogenes in accordance with other bacterial inhibitors and hygiene operations.
T. Mazaheri, C. Ripolles-Avila, A.S. Hascoët, and J.J. Rodríguez-Jerez
Elsevier BV
C. Ripolles-Avila, M. Ramos-Rubio, A.S. Hascoët, M. Castillo, and J.J. Rodríguez-Jerez
Elsevier BV
Anne-Sophie Hascoët, Carolina Ripolles-Avila, Alfons Eduard Guerrero-Navarro, and José Juan Rodríguez-Jerez
MDPI AG
There is a whole community of microorganisms capable of surviving the cleaning and disinfection processes in the food industry. These persistent microorganisms can enhance or inhibit biofilm formation and the proliferation of foodborne pathogens. Cleaning and disinfection protocols will never reduce the contamination load to 0; however, it is crucial to know which resident species are present and the risk they represent to pathogens, such as Listeria monocytogenes, as they can be further used as a complementary control strategy. The aim of this study was to evaluate the resident surface microbiota in an Iberian pig processing plant after carrying out the cleaning and disinfection processes. To do so, surface sensors were implemented, sampled, and evaluated by culture plate count. Further, isolated microorganisms were identified through biochemical tests. The results show that the surfaces are dominated by Bacillus spp., Pseudomonas spp., different enterobacteria, Mannheimia haemolytica, Rhizobium radiobacter, Staphylococcus spp., Aeromonas spp., lactic acid bacteria, and yeasts and molds. Moreover, their probable relationship with the presence of L. monocytogenes in three areas of the plant is also explained. Further studies of the resident microbiota and their interaction with pathogens such as L. monocytogenes are required. New control strategies that promote the most advantageous profile of microorganisms in the resident microbiota could be a possible alternative for pathogen control in the food industry. To this end, the understanding of the resident microbiota on the surfaces of the food industry and its relation with pathogen presence is crucial.
C. Ripolles-Avila, A.S. Hascoët, A.G. Ríos-Castillo, and J.J. Rodríguez-Jerez
Elsevier BV
A.E. Guerrero-Navarro, A.G. Ríos-Castillo, C. Ripolles Avila, A.S. Hascoët, X. Felipe, and J.J. Rodriguez Jerez
Elsevier BV
C. Ripolles-Avila, A.S. Hascoët, J.V. Martínez-Suárez, R. Capita, and J.J. Rodríguez-Jerez
Elsevier BV
C. Ripolles-Avila, B.H. Cervantes-Huaman, A.S. Hascoët, J. Yuste, and J.J. Rodríguez-Jerez
Elsevier BV
Carolina Ripolles-Avila, Maria Martinez-Garcia, Anne-Sophie Hascoët, and José Juan Rodríguez-Jerez
Informa UK Limited
ABSTRACT Two different TiO2 nanoparticles, NM101 and NM105, were evaluated against a range of Gram-positive (Staphylococcus aureus, Bacillus cereus, Lactobacillus casei, Lactobacillus bulgaricus, Lactobacillus acidophilus and Lactobacillus lactis) and Gram-negative (Salmonella enterica var. Enteridis and Escherichia coli) bacteria. Both NM101 and NM105 TiO2 nanoparticles (UV-exposed or none) had a significant antibacterial activity when the concentration of TiO2 suspension was 100 µg mL−1. The activation of the TiO2 NPs led, in all cases, to a shift in the growth curve, revealing lower counts as the concentration increased. E. coli was the most significantly affected pathogen by both TiO2 nanoparticles reaching among 2–3 log CFU.mL−1 reduction. In addition, in the case of the probiotic bacteria, NM105 TiO2 nanoparticles had similar effects as the bacterial density was reduced by 2–3 log CFU.mL−1. These results may be applied as a potent technology to be included in the formulation of new disinfectants.
C. Ripolles-Avila, A.S. Hascoët, A.E. Guerrero-Navarro, and J.J. Rodríguez-Jerez
Elsevier BV