Evaluation of Antioxidant Activity of Pulp and Seed Extracts from Garcinia cochinchinensis and Chemical Characterization of its Essential Oil Elton Kazmierczak, André Victor Bassani, Maycon Hudson Contador, Sandra Grzygorczyk, Christiana Andrade Pessôa, et al. Orbital, 2025 Garcinia cochinchinensis is native from Vietnam, used to cure allergy, itches and skin diseases. Considering the lack of studies about the chemical profile as well as the biological activities of G. cochinchinensis, this research aimed to evaluate antioxidant properties from its pulp and seeds extracts/fractions. Pulp was submitted to two different methods of extraction (liquid-liquid extraction with and without ultrasound extraction). Seed extracts were obtained with dichloromethane and ethyl acetate. Among these, the essential oil from the peel fruits of this species was analyzed by GC-MS. The antioxidant activity of extracts from pulp and seed was evaluated by the DPPH•, ABTS•+, FRAP, Iron (II) chelating methods, cyclic voltammetry and differential pulse voltammetry. Ethyl acetate in pH 8 and 4 fractions exhibited high values of phenolics and flavonoids content and showed effective antioxidant activity by the tested mechanisms. The essential oil from the peel is constituted by fatty esters and terpenes. Seed ethyl acetate extract exhibited expressive antioxidant activity in pH 6 via differential pulse voltammetry method. These promising fractions will be explored to isolate active phytochemicals and further the continuation of studies related to the evaluation of the antioxidant potential in vivo models.
Phenolic compounds and biological potential of Eugenia uniflora L.: A short review Cassia Goncalves Magalhaes, Isabela Maria Macedo Simon Sola, Aline Alberti, Jociani Ascari, Domingos Savio Nunes Ecletica Quimica, 2025 Eugenia uniflora L. (Myrtaceae) is native to Brazil and it is known as pitanga. In traditional medicine, this species is used to treat cough, skin allergies and asthma. Different parts of this plant displayed insecticidal, antimicrobial, and antioxidant activities. The main phenolic compounds found in the extracts of this species are flavonoids and tannins, which display relevant biological activities. This review shows recent phytochemical studies on E. uniflora, emphasizing the phenolic compounds, including a description of methods of extraction of these metabolites. Besides, the diversified biological activities and the potential of this plant for the food industry are reported. The pharmacological and nutraceutical potential attributed to E. uniflora justify the growing scientific interest in this species.
EXPLORING THE ANTIOXIDANT ACTIVITY OF Eugenia uniflora L. EXTRACTS: A COMPARATIVE APPROACH BY DIFFERENT TECHNIQUES Vinicius Monteiro Schaffka, Cássia Gonçalves Magalhães, Romaiana Picada Pereira Quimica Nova, 2025 Eugenia uniflora L (EU), Myrtaceae, known as pitangueira, is recognized as a source of bioactive compounds, mainly polyphenols. Considering the complexity and variety of factors that influence the composition of their extracts, assigning antioxidant properties to specimens from distinct locations and environmental conditions is important. In this study, the composition profile (total phenolic content (TPC), total flavonoid content (TFC), gas chromatography-mass spectrometry (GC-MS), and ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) fingerprint) and antioxidant activity (by chelating effect, phosphomolybdenum, 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), hypochlorous acid (HOCl), ferric reducing antioxidant power (FRAP), and cupric reducing antioxidant capacity (CUPRAC) assays) of the extracts from three specimens of pitangueira (EU1, EU2, and EU3) obtained at different pH values were compared. The results indicated that extracts obtained with ethyl acetate (EA) at pH 4 had the highest effectiveness due to their elevated levels of phenolic compounds. Additionally, a significant occurrence of flavonoids in EU2 was observed, as demonstrated by the antioxidant activity of EA at pH 8 and EA/MeOH (1:3). This study provides valuable insights into the chemical variability of E. uniflora and its significant impact on antioxidant activities, highlighting the importance of extraction conditions to enhance the therapeutic efficacy of its bioactive compounds.
DETECTION OF FLAVONOIDS WITH TRYPANOCIDAL AND ANTIOXIDANT PROPERTIES IN THE LEAVES OF Senna multijuga (FABACEAE) Cássia G. Magalhães, Ana Letícia P. dos Santos, Marcelino S. do Rosário, Elton Kazmierczak, André V. Bassani, et al. Quimica Nova, 2025 DETECTION OF FLAVONOIDS WITH TRYPANOCIDAL AND ANTIOXIDANT PROPERTIES IN THE LEAVES OF Senna multijuga (FABACEAE). Senna multijuga (Fabaceae) is a plant species native from Brazil and it has been used to recover degraded areas. There are a few reports about its chemical composition and biological activities. Therefore, this study aimed to conduct preliminary dereplication studies and evaluate the antioxidant activity by chemical and electrochemical methods of the ethanolic extract of S. multijuga (SM) leaves and its trypanocidal activity. High-performance liquid chromatography-electrospray ionization-ion trap-mass spectrometry (HPLC-ESI-IT-MS) based on molecular networking dereplication of the leaves and branches extracts led to annotation of one caffeoylquinic acid, one flavonol, two flavanones, and seven flavonol glycosides. Some of these metabolites are reported for the first time for this species. The leave extract showed significant radical scavenging properties. On the other hand, SM was more cytotoxic to 3T3 fibroblasts than to Trypanosoma cruzi epimastigotes (selectivity index (SI) = 0.23). Results showed a good agreement between the antioxidant power of extracts and their content of phenolic compounds.
Antioxidant property of secondary metabolites from Garcinia genus: A short review Elton Kazmierczak, C�ssia Gon�alves Magalh�es, Romaiana Picada Pereira Ecletica Quimica, 2023 Species from the Garcinia genus (Clusiaceae family) are used in the treatment of many diseases and metabolic disorders frequently associated with the oxidative stress. The characteristic metabolites found in this genus are xanthones and benzophenones, which have antioxidant properties, among relevant biological potentials. This review provides a specific vision about antioxidant activity of Garcinia species, reporting in vitro and in vivo assays, described from the last five years. The research about antioxidant properties of Garcinia species and their constituents can be directed in the development of new medicines and drugs.
Detection of dopamine using glassy carbon electrodes modified with AgNPs synthetized with Monteverdia ilicifolia extract Francielle Schremeta Humacayo, Joel Toribio Espinoza, Josiane de Fatima Padilha De Paula, Luma Clarindo Lopes, Christiana Andrade Pessoa, et al. Ecletica Quimica, 2023 This work reports a new application for a well-known medicinal plant used in Brazil. The green synthesis of silver nanoparticles (AgNPs) using the aqueous extract of Monteverdia ilicifolia (MI) leaves as stabilizing and reducing agent is described. The AgNPs-MI obtained were characterized by UV-VIS, FTIR, and Raman spectroscopies, DLS, zeta potential and FEG-SEM, which demonstrated that M. ilicifolia was effective at capping the AgNPs, yielding stable suspensions. These nanoparticles were deposited on glassy carbon electrodes, and they were efficiently applied as electrochemical sensors for the determination of dopamine (DA) using square wave voltammetry (SWV). The AgNPs-MI improved the electrochemical properties of the electrodes and enhanced their electroanalytical performance. The developed sensing device presented detection and quantification limits equal to 0.52 and 1.74 ?mol�L�1, respectively, towards DA determination. The proposed electrochemical sensor quantified this neurotransmitter successfully, confirming its potential as a new promising analytical detection tool for DA quality control.
Secondary metabolites and pharmacological potential of Thuja orientalis and T. occidentalis: A short review Maria Eduarda Tech, C�ssia Gon�alves Magalhães, Sidney Augusto Vieira Filho Ecletica Quimica, 2022 Species from Thuja genus (Cupressaceae) are found in Brazil, North America and Asia. In the traditional medicine, these plants are used in the treatment of cough, skin allergies, and asthma. In Brazil, Thuja species are also used in the ornamentation of urban areas. Different parts of these plants displayed insecticidal, antitumor, and antioxidant activities. The essential oil of the leaves from Thuja spp. are constituted by monoterpenes and sesquiterpenes. The main substances found in the extracts of these species are flavonoids, which display relevant biological activities. This brief review shows recent phytochemical studies involving T. orientalis and T. occidentalis, as well as 40 constituents isolated from these species. The existing pharmacological potential justifies the growing scientific interest in this genus.
Lupeol Stearate Accelerates Healing and Prevents Recurrence of Gastric Ulcer in Rodents Lincon Bordignon Somensi, Philipe Costa, Thaise Boeing, Luísa Nathália Bolda Mariano, Elizama de Gregório, et al. Evidence Based Complementary and Alternative Medicine, 2022 Objective. The focus of this study was to evaluate the gastric healing effect of lupeol stearate (LS) and its ability to minimize ulcer recurrence in rodents. Methods. To evaluate the gastric healing properties of LS, rats were subjected to 80% acetic acid-induced ulcer model and treated with vehicle, LS (1 mg/kg, p.o.), or omeprazole (20 mg/kg, p.o.), twice daily by seven days. The gastric ulcers were evaluated macroscopically, histologically, and biochemically. To evaluate the effects of LS in gastric ulcer recurrence, mice were ulcerated with 10% acetic acid and treated with vehicle, LS (1 mg/kg, p.o.), or ranitidine (100 mg/kg, p.o.), twice a day for ten days. Then, ulcer recurrence in these animals was induced by IL-1β at five days after the treatment period. Results. The oral treatment with LS accelerated gastric healing by 63% in rats compared to the vehicle group, evidenced by histological improvement and increased gastric mucin levels. Moreover, the gastric healing effects of LS in rats were accompanied by an elevation in glutathione S-transferase activity and a reduction in myeloperoxidase activity. Furthermore, the LS treatment reduced the recurred lesions in mice. Conclusions. The oral treatment of LS accelerates gastric healing in rats by favoring mucus production and reducing neutrophil migration, and it also can reduce ulcer recurrence. These data highlighted this compound as promising for developing new pharmacological strategies for the management of gastric ulcer.
