Adriana Rodrigues dos Anjos Mendonca
@univas.edu.br
Master Degree Cordinator
Vale do Sapucaí University
RESEARCH, TEACHING, or OTHER INTERESTS
Medicine, Cell Biology, Genetics, Molecular Biology
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
Renan Vinicius Pinheiro, Geraldo Magela Salomé, Flávio Dutra Miranda, José Ronaldo Alves, Fernanda Aparecida dos Reis, and Adriana Rodrigues dos Anjos Mendonça
Acta Paulista de Enfermagem
Resumo Objetivo: Elaborar e validar o conteudo de algoritmos para prevencao e tratamento de lesao por friccao. Metodos: Para a construcao dos algoritmos, foi realizada uma revisao integrativa da literatura apos busca nas bases de dados: SciELO, LILACS e MEDLINE. A avaliacao dos algoritmos foi realizada por 26 juizes, sendo 10 medicos e 16 enfermeiros, utilizando-se a tecnica Delphi. Os resultados foram analisados por meio do Indice de Validade de Conteudo. Resultados: No primeiro ciclo de avaliacao, os itens dos [...]
Rodrigo Machado Pereira, Danilo Gabriel Oliveira, Francisco Eduardo de Carvalho Costa, Fiorita Gonzales Lopes Mundim, and Adriana Rodrigues dos Anjos Mendonça
Informa UK Limited
ABSTRACT Biological staining of tissue is a crucial procedure in histotechnology. Rudimentary methods for section preparation have often used stains from natural products, although use of synthetic dyes has become the contemporary standard. Artificial dyes increase the operating costs of a laboratory as well as increase the environmental and personnel risks during manufacturing and usage. These considerations have stimulated research to find alternative natural stains from the wide diversity of plant species. The present study investigated the effect of Eucalyptus sp. (Myrtaceae) wood waste extract on histological staining of animal tissues, using a series of pigment concentrations, pH conditions, and temperatures. Eucalyptus wooden slivers were dried, milled, and 1 g, 2 g, and 4 g of the fine powder was subjected to 50% ethanol extraction for 2 days. Staining tests were then performed on formalin fixed paraffin embedded (FFPE) sections. Increasing acetic acid concentrations (1%, 2% and 4%) were added to the extracts and compared to an acid-free extract. Staining was performed at both ambient room temperature (RT) and 60°C. Connective tissue acidophilic components were well-contrasted and a hematoxylin counterstain demonstrated distinct structural differences between matrix and cell nuclei. Therefore, the present findings demonstrate the potential utility of the eucalyptus wood extracts application as a natural stain alternative for routine histology.
Imaculada Aparecida Cardoso, Geraldo Magela Salomé, Flávio Dutra Miranda, José Ronaldo Alves, João Paulo Pereira Leão, Alex Sandro Leão, and Adriana Rodrigues dos Anjos Mendonça
Georg Thieme Verlag KG
Abstract Objectives To develop an application program for prevention and treatment of complications of intestinal peristomal skin. Method The framework of the application software development were: Phase 1 – “Design, identification of the needs for the application development”: In this phase, the authors identified during their clinical practice that some professionals and caregivers find it difficult to provide care for at-risk ostomized patients or those with some kind of peristomal skin complication; Phase 2 – “Application prototype development”: this phase included the integrative literature review in the main databases; Phase 3 – “Creating the application”: this phase was intended to generate a decision tree algorithms, structure the database, and develop the software; Phase 4 – “Transition”: performing the application functionality testing. Results The application “Dermatite Periestoma App” has 36 screens and 21 figures describing the procedures for prevention and treatment of complications of intestinal peristomal skin. It is freely available from the Play Store and has been registered with the Instituto Nacional da Propriedade Industrial Ministério do Desenvolvimento, indústria e Comércio Exterior (Brazilian Ministry of Development, Industry and Foreign Trade, National Institute of Industrial Property). Conclusion This study made it possible to describe the stages of planning and development of the mobile application “Dermatite Periestoma App”. The steps taken indicate that the “Dermatite Periestoma App” has great potential for clinical practice in the evaluation of patients with stomal complications or at risk for developing peristomal skin complications, preventive measures, therapeutic approaches, and for nursing education through the use of technology.
João Batista Oliveira, Manoel Araújo Teixeira, Luiz Francisley de Paiva, Rafaela Faria de Oliveira, Adriana Rodrigues dos Anjos Mendonça, and Maria José Azevedo de Brito
Mary Ann Liebert Inc
Phytotherapy is an emerging topic of health research, with particular focus on studying the efficiency of essential oils as antimicrobials. In this study, we investigated the in vitro and in vivo susceptibility of Staphylococcus strains isolated from newborns to Cymbopogon citratus oil. The in vitro susceptibility of the microorganisms to C. citratus essential oil was compared with the activities of standard antibiotics administered to newborns using disk diffusion and microdilution methods. For in vivo assessment, 30 Wistar rats were wounded and subjected to infection of Staphylococcus aureus strain DRJ080, followed by treatment with the antibiotic vancomycin, C. citratus, or carbopol polymer gel (control) for 11 days. S. aureus accounted for 23.36% of the 107 Staphylococcus sp. strains isolated. Both vancomycin and the essential oil of C. citratus inhibited the growth of all microorganisms in vitro. The minimum inhibitory and bactericidal concentrations for the C. citratus oil were 0.625 mg/mL in all strains tested. The oil had the same therapeutic effectiveness as vancomycin against S. aureus DRJ080 in rats. Thus, Staphylococcus strains of newborns are sensitive to C. citratus oil, both in vitro and in vivo, demonstrating its potential as an antibiotic alternative.
Flávio Antônio de Melo, Carlos Américo Veiga Damasceno, Mauricéia Lins de Medeiros, Rogério Cássio Fernandes, Adriana Rodrigues dos Anjos Mendonça, and Ana Beatriz Alkmim Teixeira Loyola
Universidade de Sao Paulo, Agencia USP de Gestao da Informacao Academica (AGUIA)
Model Study: A special analytical observational prospective longitudinal and controlled study of concurrent cohort was conducted from April 2013 to November 2014. Objective: To evaluate the microbiota present in the secretion of venous ulcer patients with “Unna Boot” and their resistance to antimicrobials. Methods: secretion samples were collected from wounds of patients with and without the use of “Unna Boot” and topic therapy during the dressing change and after seven days. The isolated microorganisms were identified and tested for antimicrobial susceptibility: Result: The isolated Gram-positive microorganisms were: S. aureus, E. faecalis, S. haemolyticus and S. xylosus. Gram-negative microorganisms were E. coli, P. aeruginosa, S. plymuthica, P. mirabilis, K. pneumoniae, K. oxytoca, P. stuartii, P. vulgaris, A. hydrophila, S. marcescens, A. baumannii, E. cloacae and Tatumella sp. The growth percentage and the microbiota in the secretion of the ulcer after seven days was not significant between the two types of dressing. The resistance of Gram-positive cocci to antimicrobials in patients using the “Unna Boot” was higher than in patients without boot (p = 0.0093). Conclusion: The number of microorganisms in microbial secretion venous ulcer after dressing change is higher regardless of the type of dressing. The positive cocci increased antimicrobial resistance in patients using the “Unna Boot”.
Dênia Amélia Novato Castelli Von Atzingen, Adriana Rodrigues dos Anjos Mendonça, Marcos Mesquita Filho, Vinícius Alves Alvarenga, Vinícius Almeida Assis, Afonso Esteves Penazzo, Julio Henrique Muzetti, and Thaisa Sousa Rezende
FapUNIFESP (SciELO)
PURPOSE
To investigate the efficacy of a 10% gel of unripe banana (Musa sapientum) peel in treating surgical wounds in rats.
METHODS
A longitudinal, prospective, randomized triple-blind study was conducted with 60 Wistar rats (Rattus norvegicus albinus) weighing approximately 400g. The animals were randomly divided into: control group (treated with gel containing no active ingredient) and study group (treated with 10% gel of unripe banana peel). The gel was applied every three days to a 4x4-cm surgical wound created on the back of each animal (day 0) in both groups. Tissue samples were collected for histological analysis on days 14, 21 and 28.
RESULTS
On day 14, more extensive vascular proliferation (p=0.023), presence of mononuclear cells (p=0.000), fibroblast proliferation (p=0.012), re-epithelialization (p=0.000), and decreased presence of polymorphonuclear cells (p=0.010) were observed in the study group than in controls. No significant between-group difference in the presence of polymorphonuclear cells was found on day 21. Fibroblast proliferation was significantly greater (p=0.006) in the study group than in the control group on day 28.
CONCLUSION
The 10% gel of unripe banana peel showed anti-inflammatory activity and stimulated wound healing in rat skin when compared with a gel containing no active ingredient.
Bruna Suelen Raymundo Luz, Cristina Souza Araujo, Dênia Amélia Novato Castelli Von Atzingen, Adriana Rodrigues dos Anjos Mendonça, Marcos Mesquita Filho, and Mauricéia Lins de Medeiros
FapUNIFESP (SciELO)
BACKGROUND: Lower limb ulcers are a serious medical and socioeconomic problem throughout the world. One type of chronic wound of the lower extremities is the venous ulcer. Therapeutic methods for treating venous ulcer include the use of the Unna boot. OBJECTIVES: To evaluate the effectiveness of the customized Unna boot in the treatment of venous ulcers and to monitor the subsequent development and healing of the lesions. METHODS: Prospective exploratory and quantitative longitudinal study, conducted at the "Outpatients Department (Wound Care) of the Grupo da Fraternidade Espírita Irmão Alexandre" in the city of Pouso Alegre (MG), Brazil, in 2008. The sample consisted of 32 patients with venous ulcers who underwent treatment with the Unna boot and 11 patients (control group), who used a simple bandage application. The patients'lesions were monitored over a three month period. RESULTS: The average age of the predominently female (65.1%) patients was 61.88. From observing the differences in healing times at the three evaluation stages, it was clear that after the initial evaluation the wound area decreased in Groups 1 and 2 (p>0.05). CONCLUSION:The use of the customized Unna boot contributes to quicker healing. However, over a period of three months the simple bandage applications were seen to be just as effective as the Unna boot method.
Dênia Amélia Novato Castelli Von Atzingen, Alfredo Gragnani, Daniela Francescato Veiga, Luis Eduardo Felipe Abla, Lorraine Lorene Felix Cardoso, Thiago Ricardo, Adriana Rodrigues dos Anjos Mendonça, and Lydia Masako Ferreira
FapUNIFESP (SciELO)
PURPOSE
To assess the effects of unripe Musa sapientum peel on the healing of surgical wounds in rats.
METHODS
One hundred and twenty Wistar rats were divided into two treatment groups of 60 animals each: the control group (gel without the active ingredient) and experimental group (4% Musa sapientum peel gel). A 4 x 4 cm surgical wound was created on the back of each animal. The wound was cleaned daily with 0.9% saline, treated with 4% gel or natrosol gel (control), and covered with gauze. Animals from both groups were sacrificed after seven, 14 and 21 days of treatment; the tissue from the wound site was removed together with a margin of normal skin for histological analysis.
RESULTS
No significant differences in wound contraction rates (p=0.982) were found between time points (seven, 14 and 21 days of treatment) in both groups. However, a significantly higher wound contraction rate was observed in the control group on day 21 compared with the experimental group (p=0.029). There were no significant differences in histomorphological features between groups. The experimental group showed an increased number of polymorphonuclear cells on day 7, with a significant reduction on day 21 (p=0.026).
CONCLUSION
The use of 4% unripe Musa sapientum peel gel on surgical wounds in rats resulted in an increased number of polymorphonuclear cells on day 7, reduced wound contraction, reduced vascular proliferation and increased concentration of collagen fibers on day 21.
Dênia Amélia Novato Castelli Von Atzingen, Alfredo Gragnani, Daniela Francescato Veiga, Luis Eduardo Felipe Abla, Adriana Rodrigues dos Anjos Mendonça, Clayton Aparecido de Paula, Yara Juliano, José Carlos Correa, Marcio Raimundo de Faria, and Lydia Masako Ferreira
FapUNIFESP (SciELO)
PURPOSE: To determine the optimum concentration of a gel obtained from unripe banana (Musa sapientum) peel for wound treatment in rats. METHODS: A randomized triple blind study was conducted with 40 Wistar rats, which were divided into 4 groups: CG, control group; G2%, 2% gel concentration group; G4%, 4% gel concentration group; and G10%, 10 % gel concentration group. The banana peel gel was applied daily, for 7 days, to a 4-cm² wound created on the back of each animal of all groups. After this period, the wounds were biopsied. Statistical analysis was carried out using the Kruskal-Wallis test complemented by the Student-Newman-Keuls test. RESULTS: Macroscopic examination revealed that partial epithelialization occurred in all groups. Wound contraction was also observed in all groups and ranged from 1.38 to 1.57 mm in the study groups, and from 1.03 to 1.10 mm in the control group, with significant differences (p < 0.05) between the groups: CG and G10%, G2% and G4%, G2% and G10%. The interquartile deviation was smaller between the groups CG and G4%. CONCLUSION: The 4% gel obtained from unripe banana peel (G4%) resulted in better epithelialization of wounds healed by secondary intention compared with other gel concentrations.
A. M. V. C. Soares, S. A. Calvi, M. T. S. Peraçoli, A. C. Fernandez, L. A. Dias, and A. R. Dos Anjos
Wiley
SummaryThe effect of indomethacin (Indo), a cyclo‐oxygenase inhibitor, on the monocyte‐mediated killing of a low‐ (Pb265) and a high‐ (Pb18) virulence strain of Paracoccidioides brasiliensis was examined. The Pb18 strain was not killed by either non‐activated or interferon‐γ (IFN‐γ) ‐activated human monocytes but these cells did show fungicidal activity if pretreated with Indo. In contrast with IFN‐γ, tumour necrosis factor‐α (TNF‐α) was very effective at stimulating the fungicidal activity of monocytes. While the low‐virulence strain, Pb265, could not be killed by monocytes, cells preincubated with IFN‐γ demonstrated fungicidal activity. The killing of this strain was also induced by pretreatment of monocytes with Indo. The results suggest a negative role for prostaglandins, which are synthesized via the cyclo‐oxygenase pathway, in the regulation of monocyte‐mediated killing of virulent and avirulent strains of P. brasiliensis and that TNF‐α generation during the fungus–monocyte interaction is more important in the killing of Pb265 than Pb18.