Daniel Ribeiro da Silva McGuire

EDUCATION

PhD in Microbiology/Immunology (Aug 2014 – May 2020)
Thesis title: Studies on the immunological mechanisms leading to protection or pathology during malaria infection (

School of Biological Sciences, Nanyang Technological University & Laboratory of Pathogen Immunobiology, Singapore Immunology Network (SIgN), A*STAR, Singapore (Singapore)

MSc in Applied Microbiology (Oct 2008 – Nov 2010)
Thesis title: Selection and characterization of yeast starters from autochthonous populations in must.

Faculty of Sciences, University of Lisbon, Lisbon (Portugal)

BSc in Molecular Cell Biology and Genetics (Set 2004 – Set 2008)

RESEARCH, TEACHING, or OTHER INTERESTS

Agricultural and Biological Sciences, Applied Microbiology and Biotechnology, Plant Science, Biochemistry, Genetics and Molecular Biology

Scopus Publications

Use of immuno-flow cytometry and real-time PCR disclose the epidemiological behaviour of Erwinia amylovora populations during the winter in Portuguese pear orchards
Daniel McGuire, Telma Costa, Ana Tenreiro, Joana Cruz, Rui Sousa, et al.
Journal of Plant Pathology, 2024
The persistence and progression of Erwinia amylovora in naturally infected orchards is still not fully understood and the majority of studies have been carried out under controlled or artificial infections. A major hurdle stems from the inability to characterize the viability of the bacterium in its natural state. Most standard tests, including real-time qPCR, fail to differentiate live from dead cells, while culture dependent methods lack sensitivity. Even in orchards with recurring infections, the detection of E. amylovora is erratic. Immuno-flow cytometry (IFCM) is considered a high throughput tool in clinics and food industry but has been rarely used as diagnostic test for plant pathogens. Two pear orchards with fire blight history, located in the central part of Portugal, were selected for epidemiological monitoring between February and June, from 2019 to 2022. A total of 440 samples were tested using EPPO standard diagnostic protocols. To address the epidemiological behaviour of E. amylovora, we devised an IFCM test using specific monoclonal antibodies and viability dyes, validated in a subset of 149 samples, testing positive in routine diagnostic tests. Our IFCM test was able to differentiate live and dead E. amylovora in naturally infected samples with concentrations as low as 3.54 × 104 cell·mL−1, confirming positive results for 91% these samples, while 19.4% were considered positive by isolation, 38% using conventional PCR and 95% for real-time qPCR. Temporal analysis showed the highest number of positive samples regardless of the method employed in April, May and June, while viability values measured with IFCM were the highest in April, coinciding with the exit of dormancy and early flowering of pear trees. Integrative analysis of E. amylovora viability and population dynamics over the growing season allowed identification of distinct outbreak risks associated with these Portuguese pear orchards.
Fire4CAST – a new integrative epidemiological forecasting model for the accurate prediction of infection risk and effective control of fire blight in Pyrus orchards
Daniel McGuire, Francisco Pinto, Telma Costa, Joana Cruz, Rui Sousa, et al.
Journal of Plant Pathology, 2024
Fire blight disease, caused by Erwinia amylovora is present worldwide and affects over 40 countries in Europe where it is listed as a quarantine or regulated pest often due to ineffective control strategies maladapted to the respective production systems. In Portugal, the disease was confirmed in 2010 and the occurrence of disease outbreaks in new production areas has risen over the years. The disease affects the national production of apple and pear fruits, with greater impact on the national pear variety ‘Rocha’, widely exported to European countries and Brazil. The mild temperatures and high relative humidity promote the progression of the disease during winter, revealing the potential activity of the bacterium in the latency period (LP) of the host. Infection alert risk using the established predictive models Maryblight TM, Cougarblight and BIS98 was put in place in 2013 by Centro Operativo e Tecnológico Hortofrutícola Nacional (COTHN). These attempts to control the spread of this disease, showed low accuracy for the Portuguese epidemiological reality. Within the framework of project Fire4CAST we developed a new epidemiological model to predict fire blight disease using a systems biology approach integrating microbiological, cytological and genomic pathogen data with phenological host development and climatic variables. The presence of E. amylovora was monitored in orchards with fire blight history using standard laboratory tests. Simultaneously, the implementation of immune-flow cytometry (IFCM) highlighted the viability of E. amylovora populations prevailing during winter and early spring, long before bloom risk period. The integration of the whole data set allowed the development of the Fire4CAST predictive model, able to monitor the expected infection date (EID) and to define accurate outbreak alarms. Fire4CAST model enabled the detection of outbreak risk during winter based on rules that consider climatic data variables, which were validated by effective presence of live and active E. amylovora populations and real-time quantitative PCR (qPCR) data, accomplishing a precision rate of 83%. Field application of Fire4CAST can hopefully guide the implementation of successful control strategies, leading to more sustainable pome chain production areas.
Vaccination with sporozoites: Models and correlates of protection
Yun Shan Goh, Daniel McGuire, Laurent Rénia
Frontiers in Immunology, 2019
Despite continuous efforts, the century-old goal of eradicating malaria still remains. Multiple control interventions need to be in place simultaneously to achieve this goal. In addition to effective control measures, drug therapies and insecticides, vaccines are critical to reduce mortality and morbidity. Hence, there are numerous studies investigating various malaria vaccine candidates. Most of the malaria vaccine candidates are subunit vaccines. However, they have shown limited efficacy in Phase II and III studies. To date, only whole parasite formulations have been shown to induce sterile immunity in human. In this article, we review and discuss the recent developments in vaccination with sporozoites and the mechanisms of protection involved.
Progress in Stem Cell Therapy for Major Human Neurological Disorders
P. L. Martínez-Morales, A. Revilla, I. Ocaña, C. González, P. Sainz, et al.
Stem Cell Reviews and Reports, 2013

RECENT SCHOLAR PUBLICATIONS

Fire4CAST – a new integrative epidemiological forecasting model for the accurate prediction of infection risk and effective control of fire blight in Pyrus orchards
D McGuire, F Pinto, T Costa, J Cruz, R Sousa, ML de Sousa, C Martins, ...
Journal of Plant Pathology 106 (3), 953-966 , 2024
2024.0
Citations: 2
Use of immuno-flow cytometry and real-time PCR disclose the epidemiological behaviour of Erwinia amylovora populations during the winter in Portuguese pear …
D McGuire, T Costa, A Tenreiro, J Cruz, R Sousa, ML de Sousa, C Martins, ...
Journal of Plant Pathology 106 (3), 937-951 , 2024
2024.0
Citations: 2
FIRE4CAST–UMA NOVA FERRAMENTA PARA MONITORIZAÇÃO DE POMARES E PREVISÃO DE SURTOS DA DOENÇA DO FOGO BACTERIANO
L Cruz, R Tenreiro, A Tenreiro, F Pinto, M Gama-Carvalho, D McGuire, ...
2023.0
Vaccination with sporozoites: models and correlates of protection.
GYS Goh YunShan, D McGuire, L Rénia
2019.0
Vaccination with sporozoites: models and correlates of protection
YS Goh, D McGuire, L Rénia
Frontiers in immunology 10, 1227 , 2019
2019.0
Citations: 67
Studies on the immunological mechanisms leading to protection or pathology during malaria infection
DRS McGuire
Nanyang Technological University , 2019
2019.0
Progress in stem cell therapy for major human neurological disorders
PL Martínez-Morales, A Revilla, I Ocaña, C González, P Sainz, D McGuire, ...
Stem Cell Reviews and Reports 9 (5), 685-699 , 2013
2013.0
Citations: 157
of autochthonous yeast starter strains isolated from Portuguese musts
D MCGUIRE¹, A LEMSADDEK¹, R TENREIRO¹, F CENTENO, P PRIOR, ...
Oeno2011: Actes de colloques du 9e symposium international d'oenologie de … , 2012
2012.0
Selecção e caracterização de leveduras' starter'a partir de populações autóctones de mosto
DRS McGuire
2010.0
Citations: 7
Comparative Evaluation of the profiles of Portuguese Erwinia amylovora strains regarding CRISPR and Conventional and Emerging Control Agents
C Rodrigues, G Abreu, D McGuire, G Gomes, C Ferreira, D Araújo, ...
NOVEL SELECTION OF PORTUGUESE AUTOCHTHONOUS YEAST STRAINS ISOLATES; BEHAVIOUR STUDY IN FERMENTATIONS
BC Almeida, F Centeno, MF Teixeira, R Tenreiro, D McGuire, M Pessanha, ...
Anovel SELECTION STRATEGY APPLIED TO PORTUGUESE AUTOCHTHONOUS YEAST STRAINS ISOLATES. STUDY OF THEIR BEHAVIOR IN LAB AND REAL-LIFE FERMENTATIONS AND SENSORY ANALYSIS OF …
B CABRAL, F CENTENO, MF TEIXEIRA, R TENREIRO, D MCGUIRE, ...

MOST CITED SCHOLAR PUBLICATIONS

Progress in stem cell therapy for major human neurological disorders
PL Martínez-Morales, A Revilla, I Ocaña, C González, P Sainz, D McGuire, ...
Stem Cell Reviews and Reports 9 (5), 685-699 , 2013
2013.0
Citations: 157
Vaccination with sporozoites: models and correlates of protection
YS Goh, D McGuire, L Rénia
Frontiers in immunology 10, 1227 , 2019
2019.0
Citations: 67
Selecção e caracterização de leveduras' starter'a partir de populações autóctones de mosto
DRS McGuire
2010.0
Citations: 7
Fire4CAST – a new integrative epidemiological forecasting model for the accurate prediction of infection risk and effective control of fire blight in Pyrus orchards
D McGuire, F Pinto, T Costa, J Cruz, R Sousa, ML de Sousa, C Martins, ...
Journal of Plant Pathology 106 (3), 953-966 , 2024
2024.0
Citations: 2
Use of immuno-flow cytometry and real-time PCR disclose the epidemiological behaviour of Erwinia amylovora populations during the winter in Portuguese pear …
D McGuire, T Costa, A Tenreiro, J Cruz, R Sousa, ML de Sousa, C Martins, ...
Journal of Plant Pathology 106 (3), 937-951 , 2024
2024.0
Citations: 2
FIRE4CAST–UMA NOVA FERRAMENTA PARA MONITORIZAÇÃO DE POMARES E PREVISÃO DE SURTOS DA DOENÇA DO FOGO BACTERIANO
L Cruz, R Tenreiro, A Tenreiro, F Pinto, M Gama-Carvalho, D McGuire, ...
2023.0
Vaccination with sporozoites: models and correlates of protection.
GYS Goh YunShan, D McGuire, L Rénia
2019.0
Studies on the immunological mechanisms leading to protection or pathology during malaria infection
DRS McGuire
Nanyang Technological University , 2019
2019.0
of autochthonous yeast starter strains isolated from Portuguese musts
D MCGUIRE¹, A LEMSADDEK¹, R TENREIRO¹, F CENTENO, P PRIOR, ...
Oeno2011: Actes de colloques du 9e symposium international d'oenologie de … , 2012
2012.0
Comparative Evaluation of the profiles of Portuguese Erwinia amylovora strains regarding CRISPR and Conventional and Emerging Control Agents
C Rodrigues, G Abreu, D McGuire, G Gomes, C Ferreira, D Araújo, ...
NOVEL SELECTION OF PORTUGUESE AUTOCHTHONOUS YEAST STRAINS ISOLATES; BEHAVIOUR STUDY IN FERMENTATIONS
BC Almeida, F Centeno, MF Teixeira, R Tenreiro, D McGuire, M Pessanha, ...
Anovel SELECTION STRATEGY APPLIED TO PORTUGUESE AUTOCHTHONOUS YEAST STRAINS ISOLATES. STUDY OF THEIR BEHAVIOR IN LAB AND REAL-LIFE FERMENTATIONS AND SENSORY ANALYSIS OF …
B CABRAL, F CENTENO, MF TEIXEIRA, R TENREIRO, D MCGUIRE, ...