MOHAN GANDHI BONTHU
@vvipsgudlavalleru.ac.in
Professor
V.V. Institute of Pharmaceutical Sciences
RESEARCH, TEACHING, or OTHER INTERESTS
Pharmaceutical Science, Pharmaceutical Science
Scopus Publications
Scopus Publications
Nirmala Korukola, Girija Sastry Vedula, Mohan Gandhi Bonthu, and Lakshmana Rao Atmakuri
Asian Journal of Chemistry
This study reports the antioxidant and anticancer potential of Rhynchosia heynei, a widely used plant in traditional medicine, against various cancerous cell lines. The study found that R. heynei exhibited significant radical scavenging activity, indicating its potential as a natural antioxidant compound to guard against oxidative stress-induced diseases, including cancer. The plant extracts also displayed selective cytotoxicity towards various cell lines, with the most potent cytotoxicity exhibited by the ethanol extract towards the MCF-7 cell line and diethyl ether extract towards the HCT-116 cell line. The hexane extract exhibited substantial cytotoxic activity against HepG2, HCT-116 and L6 cell lines. However, the aqueous extract of R. heynei was the least cytotoxic of all the cell lines employed. Overall, the cytotoxic activity of R. heynei extracts was majorly selective towards MCF-7 and HCT-116. The findings suggest that R. heynei could be a potent natural product for developing anticancer drugs with fewer side effects. Further, in vitro and in vivo studies are required to determine the exact mechanism of action of the plant’s potential anticancer activity.
Arya Lakshmi Marisetti, Mohan Gandhi Bonthu, and Ganga Rao Battu
EManuscript Technologies
Background: The present work assesses the antioxidant and anti-inflammatory effect of Lasia spinosa rhizome extracts on in-vitro and in-vivo models compiled through molecular docking study of plant-steemed phytocompounds with specific targets. Materials and Methods: In this study, Lasia spinosa rhizome was subjected to extraction using petroleum ether, ethyl acetate and methanol and the extracts were analyzed by GC-MS. Antioxidant was assessed using in-vitro methods such as DPPH scavenging activity and H2O2 scavenging activity; anti-inflammatory activity was assessed using both in-vitro and in-vivo and molecular docking utilizing Auto dock 4.0 was done. Results: Tests showed that methanolic extract (MELS) has the most important dose-dependent antioxidant and anti-inflammatory efficacy at various levels. Of all compounds, Morin reported the most successful docking ranking of -8.2 to -9.8, maintaining a good binding fondness between protein and ligand. Conclusion: Antioxidant and anti-inflammatory of Lasia spinosa may be inferred from the examinations. The in-vitro, in-vivo and in silico assays of L. spinosa. Morin is confirmed by the information as a beneficial antioxidant and anti-inflammatory agent that can aid future clinical assessments.
Mohan Gandhi Bonthu, Lakshmana Rao Atmakuri, and Venkateswara Rao Jangala
FapUNIFESP (SciELO)
A simple, sensitive, rapid and highly efficient LC-MS/MS method was developed for the determination of Candesartan and Hydrochlorothiazide simultaneously in human plasma. The method employed Zorbax eclipse C18 (150 X 4.6 mm, 5µ) column using acetate buffer: acetonitrile (25:75%, v/v) as the mobile phase. The mobile phase flow rate is 1 mL/min which was delivered into the mass spectrometer electron spray ionization chamber. The Liquid/liquid extraction procedure was used in the method for the extraction of analytes. The chromatograph was attached to a negative ion mode tandem mass spectrometer and the method was validated for all the parameters as per the guidelines of US-FDA. The ions were detected in multiple reaction monitoring mode and the transitions are m/z 439.00®309.10 and 295.80®268.80 for candesartan and hydrochlorothiazide respectively. Isotopic standards were used as internal standards for effective recovery of the analytes. The drugs were analyzed over a calibration range of 1.027-302.047 ng/mL for candesartan and 1.044-306.945 ng/mL for hydrochlorothiazide respectively with regression coefficient greater than 0.99. The mean extraction recoveries are 96.95±5.61 and 100.55±4.82 for candesartan and hydrochlorothiazide respectively. The precision and accuracy values for all the studies were within the range of ≤15% and 85-115%. The performed stability studies indicate that the developed method is stable in plasma for 15 h at room temperature (bench top); 52 h (in injector); for 112 days at -70 oC for long term stability; five successive freeze and thaw cycles. The developed method could be successfully employed for the determination of selected drugs in biological samples.
B.M. Gandhi, A.L. Rao, and J.V. Rao
Elsevier BV
Bharathi Devi Y, Sumanth Srinivas Kamatham, Bhaskara Raju V, Mohan Gandhi B, and Srinivas K
Innovare Academic Sciences Pvt Ltd
Objective: This study was embarked upon to develop a new, simple, rapid, validated reversed-phase high-performance liquid chromatography (HPLC)method for the estimation of ketorolac tromethamine (KET) and tramadol hydrochloride (TDL) in pharmaceutical dosage forms.Methods: The HPLC method was developed on Shishiedo C18 column (250 mm × 4.6 mm i.d, 5 μ) using methanol: 50 mM phosphate buffer (pH 6.0) in the ratio of 52:48 at 282 nm.Results: Retention time for the drugs was found to be 5.1 and 6.9 minutes for tramadol and ketorolac, respectively. The limit of detection for tramadoland ketorolac were found to be 1.0 and 0.1 μg/ml, limit of quantitation for tramadol and ketorolac were found to be 5.0 and 0.5 μg/ml, respectively. Linearity was established in the range of 20.0-30.0 μg/ml and 8.0-12.0 μg/ml for TDL and KET, respectively. The method was precise with % relative standard deviation <2 for both intra- and interday precision. The accuracy of the method was performed over three levels of concentration, and the recovery was in the range of 98-102%.Conclusion: From the found experimental data, it can be concluded that the developed method is accurate, precise, and selective and can be employedsuccessfully for the estimation of KET and TDL in Pharmaceutical dosage forms.Keywords: Reversed-phase high-performance liquid chromatography, Ketorolac tromethamine, Tramadol hydrochloride.
Jhansi Naga Pedaprolu, Mohan Bonthu, Bhaskara Vatchavai, Srinivas Kamatham, Srinivas Kolli, and Andal Naga Kapuganti
Modestum Publishing Ltd
A stability-indicating RP-HPLC method was developed and validated for the estimation of Liraglutide in bulk and pharmaceutical dosage forms. Shiseido C18 (250 mm x 4.6 mm I.D., 5 μm particle size) column was used as stationary phase with mobile phase consisting methanol+acetonitrile (80:20): phosphate buffer (pH 3.0 adjusted with ortho phosphoric acid) in the ratio of 75:25, v/v. The flow rate was maintained at 1.2 ml/min and effluents were monitored at 245 nm. The retention time was found to be 2.837 minutes. The forced degradation studies were performed as per ICH guidelines under acidic, alkali, oxidative, thermal, photostability and neutral conditions. The drug peak was well resolved from the peaks of degraded products. From the degradation studies it is evident that the drug showed instability under acidic, alkali, oxidative, thermal, photostability and neutral conditions. The linearity of the method was observed in the concentration range of 10-60 μg/ml with the number of theoretical plates & tailing factor being 5550 & 1.17 respectively with a correlation coefficient of 0.999. The percentage assay of Liraglutide was found to be 99.66%. The method was validated for its accuracy, precision and system suitability. The results obtained in the study were within the limits of ICH guidelines and hence this method can be used for the estimation of Liraglutide in bulk and pharmaceutical dosage forms.
B. Gandhi, A. Rao, and J. Rao
Oriental Scientific Publishing Company
Novel HPTLC and stability indicating RP-HPLC methods were developed for simultaneous estimation of Moxifloxacin (MOX) and Dexamethasone (DEX) in ophthalmic dosage form. For HPTLC method, the separation was carried out on HPTLC aluminum plates using acetonitrile:water:ammonia (8:1:0.5 V/V/V) as mobile phase and developed plates were read at 266 nm. The drugs were resolved satisfactorily with Rf values of 0.09±0.01 and 0.74±0.01 for MOX and DEX, respectively. The RPHPLC analysis is carried out on Shiseido C18 column (250 mm × 4.6 mm I.D., 5 µm), using 0.02M acetate buffer (pH is 4 adjusted with triethylamine) and acetonitrile in the ratio of 60:40 V/V with a flow rate of 1.2 m/min and the detection was carried out at 254 nm. The retention times were found to be 2.144±0.5 min and 4.732±0.5 min. for MOX and DEX respectively. Developed methods were validated as per ICH guidelines and were found to be within the limits.