Malik Nurbekov

Scopus Publications

Improved Step-by-Step qPCR Method for Absolute Telomere Length Measurement
Ekaterina Sergeevna Arshinova, Nataliia Sergeevna Karpova, Olga Leonidovna Terekhina, Malik Nurbekov, Maria Ivanovna Burtovskaya
Methods and Protocols, 2026
Telomere length is a crucial marker of cellular aging and genomic stability, with significant implications for age-related diseases and cancers. This study introduces an improved quantitative PCR (qPCR) method for measuring absolute telomere length, addressing the need for accurate and high-throughput assessment in both clinical and research settings. Novel primers were designed for the single-copy gene interferon beta (IFNB1) to serve as an internal control, alongside a series of single-stranded oligonucleotide standards to establish a calibration curve. This approach allows for precise quantification of telomere length in kilobases per single copy gene copy number per chromosome. We validated this method using DNA samples from peripheral blood and buccal swabs from 17 healthy human volunteers, as well as umbilical cord blood from 9 healthy newborn babies, demonstrating its high linearity and reproducibility. Our findings indicate that this improved qPCR technique provides a rapid, cost-effective, and accurate means of measuring absolute telomere length, thereby facilitating large-scale studies and enhancing clinical diagnostics related to telomere biology.
Polymorphism rs259983 of the ZNF831 gene is associated with the risk of anemia in pregnant women with gestational diabetes
Nataliia Karpova, Olga Dmitrenko, Ekaterina Arshinova, Malik Nurbekov
Egyptian Journal of Medical Human Genetics, 2025
Background Iron deficiency is a cause of anemia in pregnant women. Iron metabolism is closely related to zinc levels and the state of zinc-containing proteins. Zinc-binding proteins (ZNFs) can also bind other metal ions. Genetic factors can also be a factor leading to unstable iron levels in the blood. Results The aim of this study was to investigate the relationship between rs259983 of the zinc finger protein 831 (ZNF831) gene and anemia in pregnant women with gestational diabetes mellitus (GDM). The PCR test system with Taq-Man samples was developed for genotyping rs259983 of the ZNF831 gene. As a result, an association was found between rs259983 of the ZNF831 gene and the risk of anemia. According to in silico analysis, the ZNF831 protein is able to bind iron (FE, 0.69) and other ions, which may play an essential role in anemia pathogenesis. Conclusions Carriers of the C allele in the homozygous state rs259983 of the ZNF831 are at greater risk of developing anemia. Further studies are required to assess the effect of ZNF831 polymorphisms on the risk of pregnancy pathologies.
Increased Preeclampsia Risk in GDM Pregnancies: The Role of SIRT1 rs12778366 Polymorphism and Telomere Length
Olga Dmitrenko, Nataliia Karpova, Malik Nurbekov
International Journal of Molecular Sciences, 2025
Preeclampsia (PE) and gestational diabetes mellitus (GDM) are common pregnancy disorders with shared pathophysiological mechanisms. This study examined the association between SIRT1 polymorphisms (rs12778366 and rs7895833) and relative telomere length (RTL) in women with PE and GDM. The DNA from pregnant women with GDM with and without PE was analyzed. The RTL and genotyping were measured using quantitative real-time PCR. The women with GDM and PE had significantly shorter telomeres. The rs12778366 TC genotype was associated with a 4.48-fold increased risk of PE (OR = 4.48; 95% CI 1.54–13.08; p = 0.003). The PE group had a higher prevalence of the heterozygous TC rs12778366 genotype with short telomeres. The SIRT1 variant rs12778366 is associated with shorter telomeres and an increased risk of developing preeclampsia, suggesting it may be a useful biomarker for preeclampsia risk assessment in GDM pregnancies.
Polymorphism rs259983 of the Zinc Finger Protein 831 Gene Increases Risk of Superimposed Preeclampsia in Women with Gestational Diabetes Mellitus
Nataliia Karpova, Olga Dmitrenko, Malik Nurbekov
International Journal of Molecular Sciences, 2024
Hypertensive disorders of pregnancy (HDP) are a great danger. A previous GWAS found a relationship between rs259983 of the ZNF831 gene and HDP, such as for chronic hypertension (CHTN) and preeclampsia (PE). We conducted the case-control study to determine the association between rs259983 of the ZNF831 gene and HDP in women with Gestational Diabetes Mellitus (GDM). For target genotyping, we developed primers and TaqMan probes. In analyzing the population, we did not manage to find a relationship between PE and rs259983 of the ZNF831 gene. Additional study of women with PE and PE superimposed on CHTN (SIPE) establishes an association between rs259983 of the ZNF831 gene only with SIPE. Carriers of CC genotypes have been discovered to have a 5.05 times higher risk of SIPE development in women with GDM.
Relative Telomere Length Is Associated with the Risk of Development and Severity of the Course of Age-Related Macular Degeneration in the Russian Population
Olga P. Dmitrenko, Olga I. Abramova, Nataliia S. Karpova, Malik K. Nurbekov, Ekaterina S. Arshinova
International Journal of Molecular Sciences, 2023
One of the most significant factors for age-related macular degeneration (AMD) development is considered to be aging, the processes of which are closely associated with telomere shortening. The different forms, indicators of aggressiveness, and intensities of AMD can be observed in the same age group, confirming the need to find a biomarker for early diagnosis and be capable of monitoring the progression of the pathological process. Therefore, we investigated whether the relative telomere length (RTL) has any connection with the risk of development of disease and its progression. RTL was measured using RT-PCR in 166 people, including 96 patients with AMD. RTL was significantly lower in patients with AMD. Women were more likely to develop AMD than men (odds ratio (OR) = 9.53 × 106 vs. OR = 1.04 × 108, respectively). The decrease in RTL in patients reliably correlated with the progression of AMD, and the smallest RTL was observed in late-stage patients. RTL < 0.8 is a significant risk factor for disease progression. The results of our research showed that RTL may be considered as a potential biomarker and a promising predictor of disease progression in patients with early AMD.
Review: Influence of 25(OH)D Blood Concentration and Supplementation during Pregnancy on Preeclampsia Development and Neonatal Outcomes
Nataliia Karpova, Olga Dmitrenko, Ekaterina Arshinova, Malik Nurbekov
International Journal of Molecular Sciences, 2022
Briefly, 25-hydroxyvitamin D (25(OH)D) plays an essential role in embryogenesis and the course of intra- and postnatal periods and is crucially involved in the functioning of the mother–placenta–fetus system. The low quantity of 25(OH)D during pregnancy can lead to an elevated risk for preeclampsia occurrence. Despite the numerous studies on the association of 25(OH)D deficiency and preeclampsia development, the current research on this theme is contradictory. In this review, we summarize and analyze study data on the effects of 25(OH)D deficiency and supplementation on pregnancy, labor, and fetal and neonatal outcomes.
Severe types of fetopathy are associated with changes in the serological proteome of diabetic mothers
Arthur T. Kopylov, Olga Papysheva, Iveta Gribova, Anna L. Kaysheva, Galina Kotaysch, et al.
Medicine United States, 2021
Pregestational or gestational diabetes are the main risk factors for diabetic fetopathy. There are no generalized signs of fetopathy before the late gestational age due to insufficient sensitivity of currently employed instrumental methods. In this cross-sectional observational study, we investigated several types of severe diabetic fetopathy (cardiomyopathy, central nervous system defects, and hepatomegaly) established in type 2 diabetic mothers during 30 to 35 gestational weeks and confirmed upon delivery. We examined peripheral blood plasma and determined a small proportion of proteins strongly associated with a specific type of fetopathy or anatomical malfunction. Most of the examined markers participate in critical processes at different stages of embryogenesis and regulate various phases of morphogenesis. Alterations in CDCL5 had a significant impact on mRNA splicing and DNA repair. Patients with central nervous system defects were characterized by the greatest depletion (ca. 7% of the basal level) of DFP3, a neurotrophic factor needed for the proper specialization of oligodendrocytes. Dysregulation of noncanonical wingless-related integration site signaling pathway (Wnt) signaling guided by pigment epithelium-derived factor (PEDF) and disheveled-associated activator of morphogenesis 2 (DAAM2) was also profound. In addition, deficiency in retinoic acid and thyroxine transport was exhibited by the dramatic increase of transthyretin (TTHY). The molecular interplay between the identified serological markers leads to pathologies in fetal development on the background of a diabetic condition. These warning serological markers can be quantitatively examined, and their profile may reflect different severe types of diabetic fetopathy, producing a beneficial effect on the current standard care for pregnant women and infants.
Analysis of Paradoxical Neurophysiological Reactions at Different Stages of the Perception of Negative Emotional Stimuli in Schizophrenia Patients
V. B. Strelets, G. I. Rodionov, M. K. Nurbekov, V. L. Ushakov, A. Yu. Arkhipov
Neuroscience and Behavioral Physiology, 2021
I/D Polymorphism Gene ACE and Risk of Preeclampsia in Women with Gestational Diabetes Mellitus
O. P. Dmitrenko, N. S. Karpova, M. K. Nurbekov, O. V. Papysheva
Disease Markers, 2020
Preeclampsia (PE) and gestational diabetes mellitus (GDM) are the most common complications of pregnancy, which result in adverse outcomes for the mother and the fetus. GDM is regarded as a separate independent risk factor for PE development, as evidenced by a higher preeclampsia rate in gestational diabetes mellitus than in the general population. The role the endothelial cell dysfunction plays is considered to be the most reasonable one in the origin of these diseases. The activity of plasma and tissue angiotensin converting enzyme (ACE) is believed to be genetically controlled. The available data suggests that increased ACE activity due to deletion (D)/insertion (I) in the 16th intron of ACE gene, which is called ACE gene I/D polymorphism, is associated with preeclampsia and varies depending on the studied population and the geography. We did not find any literature data that estimates the influence of ACE gene I/D polymorphism on PE rate in pregnant women with GDM. Therefore, the present study aimed to investigate a relationship between ACE gene I/D polymorphism and preeclampsia development in the case of GDM in the Russian population. The study used the genomic DNA derived by phenol-chloroform extraction method from venous blood samples in 137 pregnant women, including samples of 74 women with GDM accompanied with PE and the blood samples of 63 women with GDM w/o preeclampsia. Genotyping of insertion/deletion in the I/D region (16 intron of АСЕ gene) was conducted by real-time PCR using the TaqMan competing probe technology. The particular features in the frequency array of alleles and genotypes of the ACE gen I/D polymorphism under review, as associated with preeclampsia development risk in pregnant women with GDM, were identified. The acquired data testify to the need to further study of ACE gene I/D region polymorphism association in a large patient sample taking into account the PE and GDM risk factors estimated in the clinical practice.
Influence of the pgc1α, АСЕ and drd2 gene polymorphisms on the development and the course of gestational diabetes
O.V. Papysheva, M.K. Nurbekov, T.A. Mayatskaya, G.A. Kotaysh, E.N. Kozhevnikova, et al.
Voprosy Ginekologii Akusherstva I Perinatologii, 2020
Sirtuins and their role in the aging eye (review)
L. K. Moshetova, O. I. Abramova, K. I. Turkina, M. K. Nurbekov, O. P. Dmitrenko, et al.
Oftalmologiya, 2020
The influence of negative emotional stimuli on the late perception stages (P300 & N400 components) in patients with paranoid schizophrenia in implicite situation
А.Ю. Архипов, В.Ю. Новотоцкий-Власов, М. К. Нурбеков, В.Б. Стрелец
Zhurnal Vysshei Nervnoi Deyatelnosti Imeni I P Pavlova, 2018
Central and peripheral pathogenetic forms of type 2 diabetes: A proof-of-concept study
Dmitry M Davydov, Malik K Nurbekov
Endocrine Connections, 2016
Tryptophanyl-tRNA synthase gene expression as genetic marker of the athletes' overtraining
Genes and Cells, 2015
Trecrezan: Inhibitor of acid cholesterol esterase synthesis in platelets and monocytes
M. M. Rasulov, S. N. Bobkova, O. A. Belikova, M. K. Nurbekov, N. P. Shimanovskaya, et al.
Pharmaceutical Chemistry Journal, 2012
Tris-2(hydroxyethyl)ammonium (2-methylphenoxy)acetate as an inhibitor of synthesis of acid phospholipase A2 of mononuclear cells
M. M. Rasulov, S. N. Bobkova, O. A. Belikova, M. K. Nurbekov, M. G. Voronkov
Doklady Biochemistry and Biophysics, 2012
The complex of zinc bis-(2-methylphenoxyacetate) with tris-2(hydroxyethyl) amine as an activator of synthesis of total tryptophanyl-tRNA synthetase
M. M. Rasulov, M. G. Voronkov, M. K. Nurbekov, M. V. Zvereva, A. N. Mirskova, et al.
Doklady Biochemistry and Biophysics, 2012
Tris-2(hydroxyethyl)ammonium (2-methylphenoxy)acetate as an inhibitor of synthesis of acid cholesterol esterase of platelets and mononuclear cells
M. M. Rasulov, S. N. Bobkova, O. A. Belikova, M. K. Nurbekov, N. P. Shimanovskaya, et al.
Doklady Biochemistry and Biophysics, 2011
Tris-2(hydroxyethyl) ammonium 2-methylphenoxyacetate as an activator of aorta intima acid lipase
M. G. Voronkov, S. N. Bobkova, M. M. Rasulov, M. K. Nurbekov, O. A. Belikova
Doklady Biochemistry and Biophysics, 2011
Tris-2(hydroxyethyl) ammonium 2-methylphenoxyacetate as an inhibitor of synthesis of liver phospholipase A1
S. N. Bobkova, M. M. Rasulov, M. K. Nurbekov, O. A. Belikova, N. P. Shimanovskaya, et al.
Doklady Biochemistry and Biophysics, 2011
Trecrezan as an activator of aminoacyl-tRNA synthase mRNA
M. M. Rasulov, M. K. Nurbekov, S. N. Bobkova, O. A. Belikova, M. G. Voronkov
Pharmaceutical Chemistry Journal, 2011
Tris-2(hydroxyethyl)ammonium 2-methylphenoxyacetate activates the synthesis of mRNA aminoacyl-tRNA synthetase
M. K. Nurbekov, M. M. Rasulov, M. G. Voronkov, S. N. Bobkova, O. A. Belikova
Doklady Biochemistry and Biophysics, 2011
The hypolipidemic action of trecrezan and its possible molecular mechanisms
M. M. Rasulov, M. K. Nurbekov, S. N. Bobkova, V. V. Bulanova, E. S. Antonova, et al.
Pharmaceutical Chemistry Journal, 2010
Antisclerotic effect of Trekrezan and its possible mechanisms
M. G. Voronkov, M. K. Nurbekov, S. N. Bobkova, L. K. Karaulova, M. I. Susova, et al.
Doklady Biochemistry and Biophysics, 2010
Silicon in Organic and Bioorganic Chemistry: Possible Mechanisms of the Stimulating Effects of Isopropoxygermatran and 1-Ethoxysilatran in Regenerated Liver
Organosilicon Chemistry Vi from Molecules to Materials, 2008
Long-term group isolation is a factor of dysregulation of reproductive function in rats
Patologicheskaia Fiziologiia I Eksperimental Naia Terapiia, 2005
Hepatoprotective activity of 1-ethoxysilatrane and 1-isopropoxygermatrane
M. G. Voronkov, M. K. Nurbekov, M. M. Rasulov
Bulletin of Experimental Biology and Medicine, 2002

Malik Nurbekov

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