Comparison of Proximate and Fatty Acid Composition of Traditional and Value-Added Hukuti: A Fermented Fish Product Bordoloi et al. Egyptian Journal of Aquatic Biology and Fisheries, 2025 Fermented fish products are nutritionally valuable, but their quality is influenced by processing methods. This study compared traditionally prepared (TFS) and value-added (OFS) fermented fish product to assess proximate composition, mineral content, and fatty acid profiles. FS samples exhibited consistent protein (39–49%), fat (5.8–8.8%), moisture (11–13%), ash (19–23%), and carbohydrate (4–6%) contents, whereas TFS samples showed greater variability. Minerals in FS including calcium, magnesium, iron, zinc, sodium, potassium, and phosphorus were stable, while TFS levels fluctuated due to raw material and microbial variability. Fatty acid profiling revealed that OFS maintained uniform saturated and monounsaturated fatty acids, along with bioactive derivatives such as ascorbic acid esters and halogenated fatty acids. TFS samples displayed heterogeneous profiles with variable unsaturated acids, short-chain esters, and sugar-conjugated derivatives. Overall, value-added fermentation enhanced nutrient stability, lipid functionality, and reproducibility, whereas traditional methods yielded nutritionally diverse but inconsistent products. Controlled fermentation thus ensures safer, nutritionally reliable, and functionally enhanced fermented fish suitable for industrial production.
Isolation, Characterization, and Assessment of Potential Probiotic Bacteria in the Gastrointestinal Tract of Clarias magur (Hamilton, 1822) for Aquaculture Applications Das et al. Egyptian Journal of Aquatic Biology and Fisheries, 2025 Probiotic bacteria play an important role in boosting the immune system, aiding enzymatic digestion, maintaining intestinal balance, and inhibiting the growth of harmful microbes in freshwater fish. Probiotics isolated from the intestines of the same host species are generally more effective for colonization than those obtained from unrelated sources. This study evaluated the probiotic potential of gut-derived bacteria from Clarias magur. A total of 160 biological samples were collected from multiple locations across four districts in Assam, India. The tested bacterial isolates exhibited significantly different survival rates under various stress conditions. Sixteen isolates survived simulated gastrointestinal conditions (pH 2), with survival rates ranging from 60.90 to 96.30%. In bile tolerance tests, 13 isolates survived in 0.3% bile salt, showing varying tolerance levels. After one hour of exposure to 1% lysozyme (100mg/ L), all 13 isolates demonstrated good survival, with rates ranging from 63.10 to 95.50%. In the presence of 20mM hydrogen peroxide (H₂O₂), 10 isolates remained viable, with survival rates between 43.60 and 72.00%. The 13 isolates exhibited auto-aggregation abilities ranging from 45.13 to 78.70% after 5 hours of incubation, while cell surface hydrophobicity in xylene ranged from 56.55 to 92.00%. Eight isolates displayed varying degrees of antagonistic activity against four fish pathogens: Aeromonas hydrophila, Aeromonas jandaei, Pseudomonas aeruginosa, and Staphylococcus aureus. All lactic acid bacteria (LAB) isolates showed different susceptibility and resistance patterns to clinically relevant antibiotics. Importantly, all strains were γ-hemolytic, exhibiting no hemolytic activity on sheep blood agar. Molecular sequencing and phylogenetic analysis identified eight LAB isolates as Lactobacillus reuteri, Limosilactobacillus reuteri, Enterococcus faecalis, and Enterococcus mundtii. The findings highlight the probiotic potential of LAB isolated from freshwater fish and their possible application as biocontrol agents in aquaculture.
Incidence of Fish Disease in Biswanath District of Assam Kalita et al. Egyptian Journal of Aquatic Biology and Fisheries, 2025 Assam, a state in Northeast India, is rich in aquatic resources. In 2019–20, it ranked 12th in overall fish production and 7th in inland fish production in the country. As is common in aquaculture, disease outbreaks caused by pathogenic agents are frequently observed. Periodic monitoring of fish farms in the Biswanath District of Assam revealed that parasitic, bacterial, and fungal infections are prevalent. The most commonly observed diseases include parasitic infections, eye disease, fin rot, tail rot, dropsy, and epizootic ulcerative syndrome (EUS). Among these, argulosis was the most frequently reported, followed by EUS. Non-infectious diseases in fish are often caused by deficiencies and various environmental factors. Key water quality parameters—such as temperature, dissolved oxygen, pH, ammonia, nitrite, hydrogen sulfide, and heavy metals—play a crucial role in maintaining fish health, particularly in intensive and semi-intensive aquaculture systems. During the study period, the order Cypriniformes was the most affected by pathogens (49.09%), followed by Perciformes (27%), Siluriformes (13%), and Anabantiformes (10.91%). Disease outbreaks were most frequently observed shortly after stocking, during the post-rainy season, and throughout the winter. To manage disease outbreaks, the most common strategy employed by farmers was the removal of diseased fish from ponds. The use of chemical treatments was the second most common approach.
Proximate Composition of Some Traditionally Fermented Small Indigenous Fish Species of Assam Amrit Pegu, Chittaranjan Baruah Egyptian Journal of Aquatic Biology and Fisheries, 2025 Fish are widely recognized as a preferable source of protein and other essential nutrients for maintaining a healthy body, making it one of the most crucial animal protein sources. In this study, eight small indigenous fish species (SIS) were collected from various sites within the study area and "namsing”, a fermented fish product by mising the Mising tribe, was prepared to estimate their proximate composition using the techniques outlined by the Association of Official Analytical Chemists. The protein content of Amblypharyngodon mola, Puntius sophore, Mystus vittatus, Barilius barila, Gudusia chapra, Macrognathus aral, Anabas testudineus, and Nandus nandus ranged from 51.06±0.68 to 60.47±1.22g/ 100g. Lipid content was the highest in Barilius barila (22.0±0.66 g/100g) and the lowest in Nandus nandus (11.01±0.24g/ 100g). Ash content ranged from 9.61±0.41% in Anabas testudineus to 19.87±0.40% in Puntius sophore. Moisture content varied from 7.96±0.86g/ 100g in Nandus nandus to 12.38±0.41g/ 100g in Barilius barila. This study concludes that traditionally fermented fish i,e., “naming” is a rich source of essential nutrients, making it an effective food for combating malnutrition and preventing various health issues.
Amino acid and fatty acid profile of five small Indigenous fish species (SIS) from Assam, India Amrit Pegu, Chittaranjan Baruah Journal of Applied and Natural Science, 2025 Both proteins and fatty acids are significant macromolecules that control vital metabolic processes and act as building blocks for forming compounds crucial to biological processes. Fish are a significant source of high-quality protein and fatty acids in human nutrition. In this study, the amino acid fatty acid profile of five sun-dried small Indigenous fish species (SIS), viz., Anabus testudineus, Barilius barila, Gudusia chapra, Nandus nandus and Puntius sophore. The amino acid profile was determined using standard analytical techniques, while the fatty acid composition was assessed according to the AOAC method by preparing fatty acid methyl esters (FAME).The total detected essential amino acid concentrations in A. testudineus, B. barila, G. chapra, N. nandus, and P. sophore were 2788.23±5.74, 2939.86±7.6, 2750.44±12.93, 2534.41±4.77 and 1319.21±8.45µmol/L, and the concentrations of nonessential amino acids are 9141.48±6.18, 9493.55±9.58, 8523.89±13.1, 8261.06±9.96 and 4572.77±7.69 µmol/L. The study identified 23 fatty acids, with palmitic acid being the most abundant saturated fatty acid, followed by Myristic, Arachidic, Pentadecanoic, Margaric, Stearic, and Tridecanoic acids, with very low amounts of Behenic and Lignoceric acids. The present study has examined the amino acid compositions of significant dietary fish from various habitats (viz., River, beel, reservoirs, ponds and tanks, etc). Therefore, these can be recommended as a healthy supplement to the human diet, an alternate source of protein, and another nutrient requirement.
A comprehensive review of the microbial aspects of enhancing the nutritional quality of ethnic fermented fish products in Asia Ramashankar Bordoloi, Pankaj Das, Amrit Pegu, Puspakhi Borah, Naba Kumar Deka, Rupam Dutta, Chittaranjan Baruah Journal of Applied and Natural Science, 2025 Fermentation has long been utilized as a preservation method that improves the safety, longevity, and overall nutritional and sensory quality of food items such as fish. In numerous Asian regions, fermented fish products are deeply embedded in culinary traditions and contribute significantly to local food industries. The fermentation process relies on specific microbial populations that drive biochemical changes, enhancing flavor and preservation while producing compounds with potential health-promoting effects. This review presents a current assessment of fermented fish varieties found throughout Asia, focusing on traditional preparation methods, dominant microbial species, and regional practices. It also examines the bioactive properties associated with these products, including their potential to exhibit anticancer, antimicrobial, antihypertensive, and anticoagulant activities. Emphasis is placed on the functional role of fermentation-associated bacteria in developing these health benefits. Additionally, this article outlines research priorities and underscores the necessity of in vivo experiments and toxicity evaluations to ensure safety before clinical or dietary use. Continued investigation in this area holds promise for advancing human nutrition and wellness and expanding innovation within the food processing sector.
Diversity of turtles in the eastern Himalaya Biodiversity Hotspot of the Himalaya, 2024
Impact of Corn Meal and Wild Yeast Media on Drosophila Diversity in the Assam-Meghalaya Rolling Terrain Aparajita Rabha, Dhirendra K Sharma, Chittaranjan Baruah, Arup Nama Das Journal of Entomological Society of Iran, 2024 . The distribution and diversity of Drosophila species across six sites in the rolling terrain between Assam and Meghalaya, employing trap-bait and net sweeping methods throughout the year. Using corn meal and Musa balbisiana (Wild yeast mediated) as culture media, flies were collected and analyzed for abundance, richness, and diversity using Simpson, Shannon-Wiener, and Berger-Parker indices. In Baridua, Drosophila melanogaster was predominant with 968 individuals on corn meal and a richer community of 2,536 on Musa balbisiana . Tura had 844 individuals on corn meal dominated by D. melanogaster , D. elegans , D. repleta and D. sechellia while , Musa balbisiana recorded 851 individuals with D. melanogaster being dominant, alongside D. suzukii , D. sechellia , D. busckii and D. pseudoobscura . Kharkutta showed 717 individuals on corn meal predominantly D. melanogaster and D. subobscura while 949 individuals recorded in Musa balbisiana dominated by D. melanogaster , D. nasuta , D. pseudoobscura , D. subobscura and D. immigrans . Rangjuli recorded 902 individuals on corn meal with D. melanogaster , D. yakuba and D. repleta as dominant species while 1,746 individuals recorded on Musa balbisiana with D. melanogaster , D. suzukii , D. simulans and D. repleta being dominant. Dudhnoi had 825 individuals on corn meal with D. melanogaster as dominant, along with D. suzukii , D. busckii , while 2,104 individuals recorded on Musa balbisiana dominated by D. melanogaster , D. mauritiana , D. immigrans . Langpih showed 566 individuals on corn meal with D. melanogaster , D. mauritiana and D. immigrans being dominant while 834 individuals recorded on Musa balbisiana dominated by D. melanogaster , D. immigrans , D. bipectinata , D. repleta and D. suzukii . It has been found that wild yeast media significantly increased Drosophila diversity compared to corn meal. Future research should explore species genetic analysis
Impact of Wild Yeast Added to Culture Media on Drosophila Abundance in and Around a Banana Market, Daranggiri, Assam, India Aparajita Rabha, Dhirendra Sharma, Chittaranjan Baruah, Arup Das, B Achumi, et al. International Journal of Agriculture and Biosciences, 2024 In the Daranggiri area of Goalpara district, Assam, a comprehensive two-year survey was conducted to assess the abundance of Drosophila species in response to the introduction of wild yeast named bakhar in local alcohol production by the Rabha and Boro communities. The study focused on six different culture media: Corn meal (A), Musa balbisiana (B), Musa assamica (C), mixed fruit (D), Jonga (E), and Jaggery (F). Environmental factors, including temperature (26C during monsoon), relative humidity (71.23% during monsoon), rainfall (295.11 mm, monsoon), and day length (13.24 hours, June, monsoon), were recorded in the analysis. A total of 11,784 10.16 Drosophila was collected and identified across the six-culture media. Musa balbisiana (Athiya colla), the indigenous banana, exhibited the highest Drosophila abundance at 3877 3.98. Notably, the Musa balbisiana culture media attracted the highest number of Drosophila melanogaster, especially during the monsoon period. Female dominance in abundance was observed, particularly during the post-monsoon period. The study revealed variations in malefemale breakdown between different culture media. Day time abundance exceeded night time in each season, with June recording the highest daytime abundance (1331) compared to September night time abundance (858). Diversity indices, including Dominance Index (0.333 for male & 0.346 for female, monsoon), Simpson Index (0.791 for male & 0.796 for female, post monsoon), Shannon-Weinner Diversity Index(1.669 for male & 1.674 female, post monsoon), Evenness Index (0.884 for male & 0.889 for female, post monsoon), Brillouin Index (1.655 for male & 1.664 for female, post monsoon), Mehinick's Richness Index (pre monsoon male for 0.169 & 0.199 winter male)and Margalef's Index (0.705 post monsoon male & 0.734 winter male) , were analyzed across different culture media and seasons. Consistent species diversity, with six taxa in each category, was observed across seasons and genders. Monsoon showed higher species diversity according to the indices. Overall, the study provides a systematic understanding of Drosophila abundance, may be influenced by the wild yeast added to the culture media, environmental factors, and seasonal variations.
Diversity of Eel Species in Recent Evinces From Wetlands Indo-Burma Biodiversity Hotspot: Taxonomic Status, Economic Importance, and Prevailing Threats C Baruah, R Bordoloi Biodiversity Hotspot of the Indo-Burma Region, 349-365 , 2026 2026
Diversity, species richness and ethnomedicinal uses of malacofauna of varied habitats of Sonitpur district, Assam A Kalita, C Baruah Journal of Bioresources 14 (2), 17-25 , 2025 2025
Vincamine as a potential therapeutic agent for sickle cell disease: An in silico study J Sarmah, N Basumatary, P Chetia, C Baruah BRITISH JOURNAL OF HAEMATOLOGY 207, S46-S47 , 2025 2025
Comparison of Proximate and Fatty Acid Composition of Traditional and Value-Added Hukuti: A Fermented Fish Product. R Bordoloi, R Bhuyan, C Baruah Egyptian Journal of Aquatic Biology & Fisheries 29 (5) , 2025 2025
Isolation, Characterization, and Assessment of Potential Probiotic Bacteria in the Gastrointestinal Tract of Clarias magur (Hamilton, 1822) for Aquaculture Applications P Das, C Baruah, D R Egyptian Journal of Aquatic Biology and Fisheries 19 (4), 5317 – 5345 , 2025 2025
Incidence of Fish Disease in Biswanath District of Assam. R Kalita, A Pegu, C Baruah Egyptian Journal of Aquatic Biology & Fisheries 29 (4) , 2025 2025 Citations: 1
Prevalence of zoonotic diseases in the Northeastern region, one health perspective P Borah, P Das, R Bordoloi, R Begum, G Hazarika, DP Saikia, BC Chutia, ... Animals and Zoonoses 1 (2), 178-187 , 2025 2025 Citations: 7
A comprehensive review of the microbial aspects of enhancing the nutritional quality of ethnic fermented fish products in Asia R Bordoloi, P Das, A Pegu, P Borah, NK Deka, R Dutta, C Baruah Journal of Applied and Natural Science 17 (2), 677 , 2025 2025 Citations: 5
Amino acid and fatty acid profile of five small Indigenous fish species (SIS) from Assam, India A Pegu, C Baruah Journal of Applied and Natural Science 17 (2), 582 , 2025 2025 Citations: 5
Proximate Composition of Some Traditionally Fermented Small Indigenous Fish Species of Assam A Pegu, C Baruah Egyptian Journal of Aquatic Biology and Fisheries 29 (2), 2843-2853 , 2025 2025 Citations: 4
Vincamine as a Potential Therapeutic Agent for Sickle Cell Disease: A Comprehensive Computational Study B Nerswn, C Pankaj, B Chittaranjan, S Jatin Research Journal of Biotechnology 20, 3 , 2025 2025
Morphological variation in wings of Drosophila species from Assam-Meghalaya rolling terrain A Rabha, DK Sharma, C Baruah Journal of Entomological Research 49 (suppl), 1325-1329 , 2025 2025
Impact of Corn Meal and Wild Yeast Media on Drosophila Diversity in the Assam-Meghalaya Rolling Terrain A Rabha, DK Sharma, C Baruah, AN Das Journal of Entomological Society of Iran 44 (3), 329-348 , 2024 2024 Citations: 1
تاثیر پودر ذرت و مخمر وحشی بر تنوع مگس Drosophila در زمینهای ناهموار بین آسام تا مگالاپا ربحا, آپاراجیتا, شارما, دهیریندراک, باروآه, چیت تارانجان, داس, آروپ ناما نامه انجمن حشره شناسی ایران 44 (3), 329-348 , 2024 2024
Diversity of Turtles in Eastern Himalayas C Baruah, DK Sharma Biodiversity Hotspot of the Himalayas (ISBN: 978-1-00345-520-2), 245-270 , 2024 2024 Citations: 2
Probiotic bacteria as a healthy alternative for fish and biological control agents in aquaculture. P Das, P Borah, R Bordoloi, A Pegu, R Dutta, C Baruah Journal of Applied & Natural Science 16 (2) , 2024 2024 Citations: 8
Taxonomic Status, Length-Weight Relationship and Condition Factor of Channa stewartii (Playfair, 1867) in Morigaon District, Assam, India R Bordoloi, C Baruah, P Das, A Pegu Journal of Fisheries and Environment 48 (1), 83-91 , 2024 2024 Citations: 3
Graphene-Based Nanomaterials: Application in Food, Agriculture and Healthcare YK Mohanta, K Biswas, S Mahanta, S Muthupandian CRC Press , 2024 2024
Graphene-based nanomaterials YK Mohanta, Y Biswas, K Mohanta, S Mahanta IEEE Transactions on Terahertz Science and Technology 14 (5) , 2024 2024 Citations: 22
Potential of probiotic bacteria to improve silk production: Boosting the Sericulture Industry in Northeast India CB Puspakhi Borah, Pankaj Das, Ramashankar Bordoloi , Sonot Deori, Bhuban ... Journal of Applied and Natural Science 16 (4), 1431 - 1443 , 2024 2024 Citations: 2
MOST CITED SCHOLAR PUBLICATIONS
Nanopesticides: a systematic review of their prospects with special reference to tea pest management B Deka, A Babu, C Baruah, M Barthakur Frontiers in Nutrition 8, 686131 , 2021 2021 Citations: 159
Entomopathogenic microorganisms: their role in insect pest management B Deka, C Baruah, A Babu Egyptian Journal of Biological Pest Control 31 (1), 121 , 2021 2021 Citations: 155
Biological and non-conventional synthesis of zinc oxide nanoparticles (ZnO-NPs): their potential applications B Deka, C Baruah, A Babu, P Kalita Journal of Nanotechnology and Nanomaterials 3 (2), 79-89 , 2022 2022 Citations: 55
Artificial intelligence and smart agriculture technology U Kose, VB Prasath, M Mondal, P Podder, S Bharati CRC Press , 2022 2022 Citations: 46
Sequence Analysis and Structure Prediction of SARS-CoV-2 Accessory Proteins 9b and ORF14: Evolutionary Analysis Indicates Close Relatedness to Bat Coronavirus C Baruah, P Devi, DK Sharma BioMed Research International 2020 (7234961. doi: 10.1155/2020/7234961), 1-13 , 2020 2020 Citations: 45
Plant Extracts as Potential Acaricides for the Management of Red Spider Mite, Oligonychus coffeae Nietner (Acarina: Tetranychidae), in the Tea Ecosystem: An Eco … B Deka, A Babu, C Baruah, S Sarkar Frontiers in Agronomy 4, 685568 , 2022 2022 Citations: 23
Graphene-based nanomaterials YK Mohanta, Y Biswas, K Mohanta, S Mahanta IEEE Transactions on Terahertz Science and Technology 14 (5) , 2024 2024 Citations: 22
Prospects of Probiotics and Fish Growth-promoting Bacteria in Aquaculture: A Review CB Rekha Kalita, Amrit Pegu International Journal of Agriculture and Biosciences 12 (4), 234-244 , 2023 2023 Citations: 22
Nanopesticides: a systematic review of their prospects with special reference to tea pest management. Front Nutr 8: 686131 B Deka, A Babu, C Baruah, M Barthakur 2021 Citations: 17
Agricultural pest management with plant-derived nanopesticides: prospects and challenges B Deka, S Nisha, C Baruah, A Babu, S Sarkar, H Phukan Journal of Applied Nanotechnology 1 (1), 1-9 , 2022 2022 Citations: 13
Potential application of microbial resources in North-East India: Future prospects and challenges PN Bhattacharyya, C Baruah, DK Jha, DK Sharma NeBIO 2 (3), 12-18 , 2011 2011 Citations: 13
Nutritional composition of small indigenous species of fishes of Northeast India: A systematic review A Pegu, R Kalita, P Das, C Baruah Journal of Applied and Natural Science 15 (2), 649 , 2023 2023 Citations: 12
Nanopesticides: a systematic review of their prospects with special reference to tea pest management, Frontiers in Nutrition, 8: 686131 B Deka, A Babu, C Baruah, M Barthakur 2021 Citations: 12
Comparative morphometry and biogeography of the freshwater turtles of genus Pangshura (Testudines: Geoemydidae: Pangshura) C Baruah, P Devi, DK Sharma International Journal of Pure and Applied Zoology 4 (1), 107-123 , 2016 2016 Citations: 12
In silico proteome analysis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) C Baruah, P Devi, DK Sharma BioRxiv (doi: https://doi.org/10.1101/2020.05.23.104919), 2020.05. 23.104919 , 2020 2020 Citations: 10
Status and conservation of Assam roofed turtle Pangshura sylhetensis in the Brahmaputra floodplain, Assam, India C Baruah, PK Sarma, DK Sharma NeBio 1 (3), 42-47 , 2010 2010 Citations: 9
Probiotic bacteria as a healthy alternative for fish and biological control agents in aquaculture. P Das, P Borah, R Bordoloi, A Pegu, R Dutta, C Baruah Journal of Applied & Natural Science 16 (2) , 2024 2024 Citations: 8
The Emergence of Nanovaccines as a New Paradigm in Virological Vaccinology: A Review C Baruah, P Das, P Devi, PM Saikia, B Deka Exploration of Immunology 3, 361–383 , 2023 2023 Citations: 8
Entomopathogenic microorganisms: their role in insect pest management. Egypt J Biol Pest Control 31: 121 B Deka, C Baruah, A Babu 2021 Citations: 8
de Bostami. Trionyx noirâtre, La TortueNilssonia nigricans (Anderson 1875) (Ang: Black Softshell Turtle, Bostami Turtle) C Baruah, DK Sharma, AHMA Reza Chéloniens 18, 33-38 , 2010 2010 Citations: 8
