María Guembe

Scopus Publications

Clinical Efficacy and Safety of Multiple High-Dose Antibiotic-Loaded Cement Spacers in the Two-Stage Revision of Gram-Positive Periprosthetic Joint Infection
Miguel Márquez-Gómez, Lourdes Prats-Peinado, José Antonio Matas Díaz, Mar Sánchez-Somolinos, María Guembe, et al.
Microorganisms, 2026
Periprosthetic joint infection (PJI) is a devastating complication after total joint arthroplasty, typically managed through a two-stage revision protocol involving antibiotic-loaded spacers. This study aimed to evaluate the clinical outcomes and safety of a new multiple high-dose antibiotic-loaded cement (MHDALC) spacer against alternative classical antibiotic combinations for Gram-positive PJI. In this retrospective observational study of 102 patients (30 MHDALC vs. 72 control), the MHDALC cohort received spacers prepared with commercial cement (1 g clindamycin and 1 g gentamicin per 40 g) supplemented with manual additions of 4 g vancomycin and 2 g ceftazidime per 40 g of cement, while the control group received the same commercial cement supplemented with 4 g of vancomycin alone. Treatment failure was significantly lower in the MHDALC group (6.6%) compared to the control group (20.8%; p = 0.005). Furthermore, the time to second-stage reimplantation was drastically reduced in the MHDALC cohort (9.1 vs. 17.8 weeks; p = 0.001). Despite the substantially higher antibiotic load, no significant differences were observed regarding mechanical or surgical complications between the two groups (p = 1.00). In conclusion, the use of MHDALC spacers is an effective and safe strategy for treating Gram-positive PJI, significantly improving eradication rates and accelerating the transition to definitive reconstruction without compromising structural integrity.
Corrigendum to “In vitro study to assess modulation ofCandidabiofilm byEscherichia colifrom vaginal strains” [Biofilm 5 (2023) 100116]
Marta Díaz-Navarro, Álvaro Irigoyen, María Palomo, Pilar Escribano, Jesús Guinea, et al.
Biofilm, 2025
Biomass and metabolic activity staining biofilm techniques are not reliable enough to be used in microbiology laboratories
Marta Díaz-Navarro, Ana Crespo, Patricia Muñoz, María Guembe
Journal of Microbiological Methods, 2025
Quantification and Comparison of Different Biofilm Components from Methicillin-Susceptible Staphylococcus aureus Treated with Tranexamic Acid Using an In Vitro Model
Marta Díaz-Navarro, Antonio Benjumea, Andrés Visedo, Patricia Muñoz, Javier Vaquero, et al.
Microorganisms, 2025
As we previously demonstrated that tranexamic acid (TXA), an antifibrinolytic, showed an antibacterial effect alone and in combination with vancomycin and gentamicin, we now wanted to analyze its own efficacy using new, different fluorescent staining reagents that target different components of the biofilm matrix and compare which one quantifies biofilm reduction better. A 108 cfu/mL suspension of the Staphylococcus aureus (ATCC29213) strain was placed into the wells of a 24-multiwell plate covered with glass slides coated with 10% poly-L-lysine under agitation for 24 h at 37 °C. After 3 washes with PBS, wells were treated with either TXA 10 mg/mL or sterile water and incubated for 24 h at 37 °C. After three washes with PBS, the density area of the following biofilm components was calculated using confocal laser scanning microscopy: extracellular proteins (Sypro Ruby), α-extracellular polysaccharides (ConA-Alexa fluor 633), α or β-extracellular polysaccharides (GS-II-Alexa fluor 488), bacterial DNA (PI), and eDNA (TOTO®-1). We observed a statistically significant reduction in the occupied area by all components of the S. aureus biofilm (p < 0.001) after TXA 10 mg/mL treatment, compared to the positive control. All biofilm components’ reduction percentages reached ≥90.0%. We demonstrated that TXA reduced both bacteria and extracellular matrix components of S. aureus biofilm by using five different stain reagents, with all being equally valid for quantification.
Assessment of the Anti-Biofilm Effect of Cefiderocol Against 28 Clinical Strains of Multidrug-Resistant Gram-Negative Bacilli
Marta Díaz-Navarro, Emilia Cercenado, Andrés Visedo, Mercedes Marín, Marina Machado, et al.
Antibiotics, 2025
Objectives: Cefideroccol (FDC) is a siderophore cephalosporin with potent antibacterial activity against a wide range of Gram-negative multidrug-resistant (MDR) microorganisms. We investigated the anti-biofilm capacity of FDC against clinical strains. Methods: This multicenter study was conducted on 28 selected strains of MDR Gram-negative bacilli isolated from clinical samples of Pseudomonas aeruginosa (n = 5), Acinetobacter baumannii (n = 11), and Klebsiella pneumoniae (n = 12). We first determined the minimum inhibitory concentration (MIC) of each strain using the microdilution method. We also defined the minimum biofilm inhibitory concentration (MBIC) as a ≥50% reduction in tetrazolium salt (XTT) (as recommended in the 2017 Spanish Microbiology Protocols [SEIMC] for the microbiological diagnosis of infections related to the formation of biofilms). We also analyzed the reduction in the following biofilm variables after an 8 mg/mL FDC treatment: the CFU count, the cell viability, the biomass, the metabolic activity, and extracellular α or β polysaccharides. Results: The MIC50 and MBIC50 of FDC were 0.5 mg/L and 64 mg/L, respectively. We observed a mean (SD) fold increase in the susceptibility to FDC between planktonic and sessile cells for P. aeruginosa, A. baumannii, and K. pneumoniae of 9.60 (0.55), 6.27 (2.28), and 6.25 (2.80), respectively. When 8 mg/mL of FDC was tested, we observed that the best median (IQR) percentage reductions were obtained for cell viability and the extracellular matrix (73.1 [12.4–86.5] and 79.5 [37.3–95.5], respectively), particularly for P. aeruginosa. The lowest percentage reduction rates were those obtained for biomass. Conclusions: We demonstrated that the susceptibility to FDC was significantly reduced when strains were in a biofilm state. The best percentage reduction rates for all biofilm-defining variables were observed for P. aeruginosa. Our results need to be validated using a larger collection of clinical samples.
Surveillance of nasal Staphylococcus aureus in patients undergoing breast surgery
Ma Jesús Pérez-Granda, Andrés Visedo, Martín Olivares, Álvaro García-Cañal, Marta Díaz-Navarro, et al.
Microbiology Spectrum, 2025
Screening and decolonization programs have proven effective in reducing the frequency of Staphylococcus aureus infections, mainly in orthopedic and cardiac procedures. Despite being classified as clean, breast surgery is associated with infection. Using culture and polymerase chain reaction (PCR) assay, we aimed to assess the frequency of nasal carriage of S. aureus in patients undergoing breast surgery. We conducted a prospective observational 10-month study at a large tertiary teaching hospital, including patients undergoing breast reconstruction surgery who met the eligibility criteria and signed the informed consent document. Nasal swabs were collected before surgery from patients with no signs of S. aureus infection and tested using both culture and the Xpert MRSA/SA SSTI PCR assay. The outcomes were nasal colonization by S. aureus , colonization rates at the time of surgery, infection, and length of hospital stay. We included 100 patients, 27% of whom were colonized. Of these, 20 patients were positive by culture and 27 by PCR. The median (IQR) age was 56.0 (49.0–63.7) years. A total of 6 patients had infection, and the median (IQR) number of days until onset of infection was 191 (186.25–197.00). Carriage of S. aureus before surgery was microbiologically confirmed in two patients. We demonstrated that 27% of patients undergoing breast surgery were nasal carriers of S. aureus , with PCR assay being the best diagnostic strategy. Future studies are needed to address the efficacy of bacterial decolonization of the nose and nipples to reduce the frequency of infection and complications. IMPORTANCE We showed that almost 30% of women undergoing breast surgery are nasal carriers of S. aureus , and PCR molecular technique was the best diagnostic tool. However, future studies are needed to implement decolonization of nasal and nipple to reduce infection and complications.
Is It Possible to Optimize the Elaboration and Preservation of a Vancomycin Catheter Lock Solution?
Marta Díaz-Navarro, David Samitier, Félix García-Moreno, María Sanjurjo, Patricia Muñoz, et al.
Antibiotics, 2025
Background/Objectives: Vancomycin (V) is widely used for catheter lock therapy. However, its ad hoc preparation in pharmacy departments involves discarding most of an intravenous vial and contributes to high workload. We aimed to assess the V concentration and minimum inhibitory biofilm concentration (MIBC) of a frozen V lock solution. Methods: Two V-2 mg/mL solutions were tested: (1) V + heparin 100 IU/mL and (2) V + citrate 2%. Solutions were frozen at −20 °C, followed by 48 h refrigeration, and analyses were performed at baseline and after 2, 4, 8, and 12 weeks (experiment 1). In addition, after the 12-week freezing period, solution 1 was also preserved for 1 and 2 weeks at both 4 °C and room temperature (experiment 2). V concentration was assessed by HPLC-DAD at 205 nm and validated with forced degradation tests. A <10% variation indicated significant change. MBIC was determined by XTT staining of 24 h biofilms exposed to decreasing concentrations of each solution. Microorganisms tested included methicillin-susceptible and -resistant Staphylococcus aureus (MSSA, MRSA), Staphylococcus epidermidis ATCC35984 (SE), and a highly biofilm-forming clinical S. epidermidis strain (SEclin). MIBC was defined as ≥50% reduction in metabolic activity. Results: In experiment 1, while V concentration remained stable over time, MIBC values varied, notably increasing from 8 weeks for all strains. Moreover, in experiment 2, significant reductions in both V concentration and MIBC were detected in the 2-week period. Conclusions: V lock solution appears to be able to be 12-weeks frozen followed by up to 1 week at refrigeration or room temperature. This facilitates the optimization of vial preparation in hospital pharmacy laboratories.
Reliability of differential time to positivity technique for diagnosing catheter-related bloodstream infections: a retrospective analysis
Álvaro Irigoyen-von-Sierakowski, Marta Díaz-Navarro, Andrés Visedo, María Jesús Pérez-Granda, Pablo Martín-Rabadán, et al.
Microbiology Spectrum, 2025
The differential time to positivity (DTTP) technique is the recommended conservative procedure to diagnose catheter-related bloodstream infection (C-RBSI). However, its reliability and accuracy remain under debate. Therefore, we aimed to compare the DTTP technique feasibility to detect C-RBSI compared to the catheter culture (CC) method. We conducted a 9-month retrospective study including bacteremic episodes in which both DTTP blood cultures (BC) and CC were obtained. We analyzed the diagnostic validity of the DTTP technique for detecting C-RBSI compared to the gold standard (C-RBSI with CC), along with patient clinical data. We included 37 episodes of C-RBSI where both DTTP BC and CC were obtained. C-RBSI was confirmed by both techniques in only 13 episodes (35.1%), whereas in 11 (29.7%) and 13 (35.1%), only DTTP BC or DTTP BC with CC (with a difference between catheter lumen and peripheral BC growth of <2 hours) was positive, respectively. Therefore, the validity values of the DTTP technique for predicting C-RBSI were as follows: sensitivity, 50.0%; specificity, 71.8%; positive predictive value, 54.2%; and negative predictive value, 68.3%. The distribution of microorganisms was similar among the three groups. All patients in whom colonization was not demonstrated by CC ( n = 11) had been receiving antibiotics before catheter withdrawal. DTTP is a conservative technique that might help to diagnose C-RBSI mostly in situations where catheter removal cannot be achieved. However, it should be interpreted with caution and never be used to rule out C-RBSI. CC before starting antimicrobial therapy remains the most reliable method to diagnose and confirm an episode of C-RBSI. IMPORTANCE We try to clarify the reliability of the differential time to positivity technique to predict C-RBSI. It may be interpreted with caution and considering clinical signs, as some C-RBSI can be misdiagnosed.
Virulence profile of carbapenem-resistant Klebsiella pneumoniae strains by an in vivo model of Galleria mellonella
María Guembe, Rama Hafian, Marta Díaz-Navarro, Andrés Visedo, Flavio De Maio, et al.
Microbiology Spectrum, 2025
Klebsiella pneumoniae is a significant healthcare-associated pathogen, notable for its diverse virulence and antibiotic resistance profiles. This study aimed to characterize the genotypic and phenotypic diversity of K. pneumoniae isolates and evaluate their virulence using the Galleria mellonella model. Biomass production, metabolic activity, capsule formation, and siderophore production were assessed in 27 K . pneumoniae isolates from hospital-associated infections. Lethality curves were generated using the G. mellonella model, with survival monitored hourly from 16 to 48 hours. The most common sequence types (ST) identified were the high-risk clones ST307 ( N = 10), ST512 ( N = 8), ST101 ( N = 7), and ST661 ( N = 2). These STs were associated with distinct K-locus, including KL102, KL107, KL17, and KL39. Most isolates belonged to the O2afg locus ( N = 18), with the K. pneumoniae carbapenemase genotype detected in 96.3% of strains. None of the isolates were classified as hypervirulent. Phenotypically, ST661 exhibited the highest biomass production despite showing similar metabolic activity to other STs. A positive correlation was observed between biomass and siderophore production, while capsule production was inversely correlated with biomass. In the G. mellonella model, ST661 demonstrated the highest virulence, resulting in 100% mortality by 48 hours, compared to survival rates of 21.4% for ST101, 38.0% for ST307, and 31.2% for ST512. These findings underscore the pathogenic potential of ST661 isolates with enhanced biofilm production. The G. mellonella model may serve as an effective in vivo system for evaluating the virulence of emerging K. pneumoniae lineages. IMPORTANCE We demonstrate that the Galleria mellonella model is a useful tool to analyze the virulence of carbapenem-resistant Klebsiella pneumoniae strains. Our findings highlight the pathogenicity of carbapenem-resistant K pneumoniae isolates, particularly the role of the ST661 that, despite being a rare lineage, harbors the blaVIM gene and is associated with high biofilm production and the highest mortality rates.
Is an oritavancin catheter lock solution active against biofilms of staphylococci and enterococci?
Marta Díaz-Navarro, Emilia Cercenado, Ariadna Monte, Andrés Visedo, Carmen Rodríguez, et al.
Heliyon, 2025
99mTc-DTPA-Collagen Radiotracer for the Noninvasive Detection of Infective Endocarditis
Mario González-Arjona, Gorka Sobrino, Lorena Cussó, María Guembe, Daniel Calle, et al.
ACS Infectious Diseases, 2025
Correction: Assessment of the anti-biofilm effect of UV-C irradiation (254 nm) against healthcare associated infections related microorganisms (Frontiers in Microbiology, (2025), 16, (1570334), 10.3389/fmicb.2025.1570334)
Francesco Palma, Marta Díaz-Navarro, Andrés Visedo, Pablo Sanz-Ruíz, Giorgio Brandi, et al.
Frontiers in Microbiology, 2025
Assessment of the anti-biofilm effect of UV-C irradiation (254 nm) against healthcare associated infections related microorganisms
Francesco Palma, Marta Díaz-Navarro, Andrés Visedo, Pablo Sanz-Ruíz, Giorgio Brandi, et al.
Frontiers in Microbiology, 2025
New insights in the role of Candida biofilm in the pathogenesis of recurrent vulvovaginal candidiasis: a prospective clinical study
Marta Díaz-Navarro, Álvaro Irigoyen-von-Sierakowski, Imani Delcán, Ariadna Monte, María Palomo, et al.
Frontiers in Microbiology, 2025
Does a Specific Sequential Combination of Antiseptic Solutions for Chemical Debridement in Periprosthetic Joint Infection Improve Outcomes vs. Solution Alone? An In Vivo Study
Miguel Márquez-Gómez, Marta Díaz-Navarro, Andrés Visedo, Lourdes Prats-Peinado, Patricia Muñoz, et al.
Antibiotics, 2024
The role of tranexamic acid for infection prevention after fracture fixation
Antonio Benjumea-Carrasco, María Guembe, Marta Díaz-Navarro, Patricia Muñoz, Javier Vaquero-Martin, et al.
Injury, 2024
Addressing catheter lock therapy: Does heparin reduce the bioactivity of dalbavancin when together in solution during freezing?
Marta Díaz-Navarro, Rama Hafian, María Jesús Pérez-Granda, Emilia Cercenado, Patricia Muñoz, et al.
Enfermedades Infecciosas Y Microbiologia Clinica, 2024
Vascular catheter-related infections: an endemic disease in healthcare institutions. An opinion paper of the Spanish Society of Cardiovascular Infections (SEICAV)
Patricia Muñoz, María Guembe, María Jesús Pérez-Granda, José Luís del Pozo, Luis Eduardo López-Cortés, et al.
Revista Espanola De Quimioterapia, 2024
The endoscopy department can alert to complications associated with peripheral venous catheters in patients admitted to a tertiary teaching hospital
María Jesús Pérez-Granda, Francisca Guzmán Blanco, Sonia Aguado Díaz, Rosario Jiménez Bautista, Julia Orense Velilla, et al.
Heliyon, 2024
Impact of the COVID-19 pandemic on the incidence and the epidemiology of catheter-related bloodstream infection two years later
María Jesús Pérez-Granda, Almudena Burillo, Julia Serrano-Lobo, Pablo Martín-Rabadán, Patricia Muñoz, et al.
Heliyon, 2024
Clinical Outcome of Patients with Escherichia coli Isolated from Catheter Lumens and/or Peripheral Blood Cultures: A Retrospective Analysis
Álvaro Irigoyen-von-Sierakowski, Marta Díaz-Navarro, Andrés Visedo, Mª Jesús Pérez-Granda, Pablo Martín-Rabadán, et al.
Pathogens, 2024
Biofilm-Related Infections in Healthcare: Moving towards New Horizons
Enea Gino Di Domenico, Alessandra Oliva, María Guembe
Microorganisms, 2024
Impact of an interventional bundle on complications associated with peripheral venous catheters in elderly patients
M. Pérez-Granda, Álvaro Irigoyen-von-Sierakowski, Neera Toledo, Eva Rodríguez, María Luisa Cruz, et al.
European Journal of Clinical Microbiology and Infectious Diseases, 2024
Editorial: New insights in prosthetic joint infections
María Guembe, Nils P. Hailer, Pablo Sanz-Ruíz
Frontiers in Microbiology, 2024
Corrigendum: Tranexamic acid in combination with vancomycin or gentamicin has a synergistic effect against staphylococci (Front. Microbiol., (2023), 13, 935646, 10.3389/fmicb.2022.935646)
Antonio Benjumea, Marta Díaz-Navarro, Rama Hafian, Emilia Cercenado, Mar Sánchez-Somolinos, et al.
Frontiers in Microbiology, 2024
Validation of the antibacterial effect of topically applied tranexamic acid using in vitro and in vivo models
Antonio Benjumea, Marta Díaz-Navarro, Ángela Sai Gago-Campos, Andrés Visedo, Rama Hafian, et al.
Frontiers in Microbiology, 2024
In vitro study to assess modulation of Candida biofilm by Escherichia coli from vaginal strains
Marta Díaz-Navarro, Álvaro Irigoyen Von-Sierakowski, María Palomo, Pilar Escribano, Jesús Guinea, et al.
Biofilm, 2023
An In Vitro Study to Assess the Best Strategy for the Chemical Debridement of Periprosthetic Joint Infection
Miguel Márquez-Gómez, Marta Díaz-Navarro, Andrés Visedo, Rama Hafian, José Matas, et al.
Antibiotics, 2023
In Vitro Efficacy of Dalbavancin as a Long-Acting Anti-Biofilm Agent Loaded in Bone Cement
Mar Sánchez-Somolinos, Marta Díaz-Navarro, Antonio Benjumea, José Matas, Javier Vaquero, et al.
Antibiotics, 2023
Determination of the ability of matrix-assisted laser desorption ionization time-of-flight mass spectrometry to identify high-biofilm-producing strains
David Rodríguez-Temporal, Rafael Díez, Marta Díaz-Navarro, Pilar Escribano, Jesús Guinea, et al.
Frontiers in Microbiology, 2023
Understanding the diagnosis of catheter-related bloodstream infection: real-time monitoring of biofilm growth dynamics using time-lapse optical microscopy
Marta Díaz-Navarro, Rafael Samaniego, Juan Carlos Piqueras, Rafael Díez, Rama Hafian, et al.
Frontiers in Cellular and Infection Microbiology, 2023
Production of biofilm by Staphylococcus aureus: Association with infective endocarditis?
Beatriz Alonso, María Jesús Pérez-Granda, María Consuelo Latorre, Carlos Sánchez-Carrillo, Emilio Bouza, et al.
Enfermedades Infecciosas Y Microbiologia Clinica, 2022
Determination of the Elution Capacity of Dalbavancin in Bone Cements: New Alternative for the Treatment of Biofilm-Related Peri-Prosthetic Joint Infections Based on an In Vitro Study
Mar Sánchez-Somolinos, Marta Díaz-Navarro, Antonio Benjumea, Marta Tormo, José Matas, et al.
Antibiotics, 2022
Exhaustive diagnosis of breast implants with capsular contracture: The microbiology laboratory as a major support
Michele Castellano, Mercedes Marín, Luis Alcalá, Iskra Cunnas, Belén Rodríguez, et al.
Journal of Plastic Reconstructive and Aesthetic Surgery, 2022
The Current Knowledge on the Pathogenesis of Tissue and Medical Device-Related Biofilm Infections
Enea Gino Di Domenico, Alessandra Oliva, María Guembe
Microorganisms, 2022
Tranexamic Acid in Combination With Vancomycin or Gentamicin Has a Synergistic Effect Against Staphylococci
Antonio Benjumea, Marta Díaz-Navarro, Rama Hafian, Emilia Cercenado, Mar Sánchez-Somolinos, et al.
Frontiers in Microbiology, 2022
Correction to: A Dalbavancin Lock Solution Can Reduce Enterococcal Biofilms After Freezing (Infectious Diseases and Therapy, (2022), 11, 2, (743-755), 10.1007/s40121-021-00579-4)
Marta Díaz-Navarro, Rama Hafian, Irene Manzano, María J. Pérez-Granda, Emilia Cercenado, et al.
Infectious Diseases and Therapy, 2022
The role of biofilm production in Cutibacterium acnes strains isolated from breast implants
María Consuelo Latorre, Luis Alcalá, Michele Castellano, José Lasso, Rosa Pérez-Cano, et al.
Journal of Plastic Reconstructive and Aesthetic Surgery, 2022
A Dalbavancin Lock Solution Can Reduce Enterococcal Biofilms After Freezing
Marta Díaz-Navarro, Rama Hafian, Irene Manzano, María J. Pérez-Granda, Emilia Cercenado, et al.
Infectious Diseases and Therapy, 2022
Role of Extracellular DNA in Dalbavancin Activity against Methicillin-Resistant Staphylococcus aureus (MRSA) Biofilms in Patients with Skin and Soft Tissue Infections
Francesca Sivori, Ilaria Cavallo, Daniela Kovacs, Maria Guembe, Isabella Sperduti, et al.
Microbiology Spectrum, 2022
Efficacy of Povidone Iodine Against Microbial Biofilms in Breast Implants With Different Textures: Results From an in vitro Study
Borja Fernández-Ibarburu, Marta Díaz-Navarro, Gorka Ibarra, Andrés Rivera, Rama Hafian, et al.
Frontiers in Microbiology, 2022
The classification of Staphylococcus aureus strains by biofilm production differs depending on the method used
María Consuelo Latorre, Beatriz Alonso, Raquel Cruces, Aurora Sanz, Patricia Muñoz, et al.
Enfermedades Infecciosas Y Microbiologia Clinica, 2022
Effect of Tranexamic Acid against Staphylococcus spp. and Cutibacterium acnes Associated with Peri-Implant Infection: Results from an In Vitro Study
Antonio Benjumea, Marta Díaz-Navarro, Rama Hafian, Mar Sánchez-Somolinos, Javier Vaquero, et al.
Microbiology Spectrum, 2022
Editorial: Microbial Biofilms in Chronic and Recurrent Infections
Fiorentina Ascenzioni, Axel Cloeckaert, Enea Gino Di Domenico, Catherine Dunyach-Remy, María Guembe
Frontiers in Microbiology, 2021
Endotracheal tubes coated with a broad-spectrum antibacterial ceragenin reduce bacterial biofilm in an in vitro bench top model
María Consuelo Latorre, María Jesús Pérez-Granda, Paul B Savage, Beatriz Alonso, Pablo Martín-Rabadán, et al.
Journal of Antimicrobial Chemotherapy, 2021
How should microbiology laboratories interpret cultures of the sonicate of closed needleless connectors?
María Jesús Pérez-Granda, José María Barrio, Raquel Cruces, Beatriz Alonso, Pablo Martín-Rabadán, et al.
Enfermedades Infecciosas Y Microbiologia Clinica, 2021
In Vitro Study to Evaluate the Bioactivity of Freezing a Heparin-Based Dalbavancin Lock Solution
Marta Rubia, Andrea Cordero, María Jesús Pérez-Granda, Emilia Cercenado, Cristina Pascual, et al.
Antimicrobial Agents and Chemotherapy, 2020
Correction to: Characterization of the virulence of Pseudomonas aeruginosa strains causing ventilator-associated pneumonia (BMC Infectious Diseases, (2020), 20, 1, (909), 10.1186/s12879-020-05534-1)
Beatriz Alonso, Laia Fernández-Barat, Enea Gino Di Domenico, Mercedes Marín, Emilia Cercenado, et al.
BMC Infectious Diseases, 2020
Characterization of the virulence of Pseudomonas aeruginosa strains causing ventilator-associated pneumonia
Beatriz Alonso, Laia Fernández-Barat, Enea Gino Di Domenico, Mercedes Marín, Emilia Cercenado, et al.
BMC Infectious Diseases, 2020
Selective digestive decontamination solution used as "lock therapy" prevents and eradicates bacterial biofilm in an in vitro bench-top model
María Jesús Pérez-Granda, Beatriz Alonso, Ricardo Zavala, María Consuelo Latorre, Javier Hortal, et al.
Annals of Clinical Microbiology and Antimicrobials, 2020
Can vancomycin lock therapy extend the retention time of infected long-term catheters?
Beatriz Alonso, Ana Fernández‐Cruz, Marta Díaz, Carlos Sánchez‐Carrillo, Pablo Martín‐Rabadán, et al.
APMIS, 2020
What does really affect the colonization of needleless connectors?
María Guembe, María Jesús Pérez-Granda
Enfermedades Infecciosas Y Microbiologia Clinica, 2020
Randomized clinical trial analyzing maintenance of peripheral venous catheters in an internal medicine unit: Heparin vs. saline
María Jesús Pérez-Granda, Emilio Bouza, Blanca Pinilla, Raquel Cruces, Ariana González, et al.
Plos One, 2020
Microbiological diagnosis of catheter-related infections
Carmen Aldea Mansilla, José Martínez-Alarcón, Irene Gracia Ahufinger, María Guembe Ramírez
Enfermedades Infecciosas Y Microbiologia Clinica, 2019
Evaluation of the Alfred™ turbidity monitoring system (Alifax®) following sonication in the diagnosis of central venous catheter colonization
Beatriz Alonso, María Consuelo Latorre, Raquel Cruces, David Ampuero, Laura Haces, et al.
European Journal of Clinical Microbiology and Infectious Diseases, 2019
Correction: Is heparinized 40% ethanol lock solution efficient for reducing bacterial and fungal biofilms in an in vitro model(PLOS ONE(2019) 14:8(e0221702)DOI:10.1371/journal.pone.0219098)
Beatriz Alonso, María Jesús Pérez-Granda, María Consuelo Latorre, Carmen Rodríguez, Carlos Sánchez-Carrillo, et al.
Plos One, 2019
Is heparinized 40% ethanol lock solution efficient for reducing bacterial and fungal biofilms in an in vitro model?
Beatriz Alonso, María Jesús Pérez-Granda, María Consuelo Latorre, Carmen Rodríguez, Carlos Sánchez-Carrillo, et al.
Plos One, 2019
Assessment of the anti-biofilm effect of micafungin in an animal model of catheter-related candidemia
Beatriz Salinas, María Guembe, Lorena Cussó, Martha Kestler, Jesús Guinea, et al.
Medical Mycology, 2019
In vitro assessment of the anti-biofilm activity of ethanol alone and in combination with enoxaparin 60 IU
María Jesús Pérez-Granda, Beatriz Alonso, Ana Rodríguez-Huerta, Carmen Rodríguez, María Guembe
Enfermedades Infecciosas Y Microbiologia Clinica, 2018
The optimal ethanol lock therapy regimen for treatment of biofilm-associated catheter infections: an in-vitro study
B. Alonso, M.J. Pérez-Granda, A. Rodríguez-Huerta, C. Rodríguez, E. Bouza, et al.
Journal of Hospital Infection, 2018
The role of time to positivity of blood cultures on the diagnosis of true CoNS bacteraemia
María Guembe, Carlos Sánchez-Carrillo
Infectious Diseases, 2018
Can dalbavancin be used as a catheter lock solution?
Cristina Díaz-Ruíz, Beatriz Alonso, Emilia Cercenado, Raquel Cruces, Emilio Bouza, et al.
Journal of Medical Microbiology, 2018
Impact of a training program on adherence to recommendations for care of venous lines in internal medicine departments in Spain
in behalf of the NUVE Study Group, María Guembe, María Jesús Pérez-Granda, José Antonio Capdevila, José Barberán, et al.
European Journal of Clinical Microbiology and Infectious Diseases, 2018
Biofilm production is not associated with poor clinical outcome in 485 patients with Staphylococcus aureus bacteraemia
M. Guembe, B. Alonso, J. Lucio, M.J. Pérez-Granda, R. Cruces, et al.
Clinical Microbiology and Infection, 2018
The clinical Biofilm Ring Test: A promising tool for the clinical assessment of biofilm-producing Candida species
Enea Gino Di Domenico, Ilaria Cavallo, Maria Guembe, Grazia Prignano, Maria Teresa Gallo, et al.
FEMS Yeast Research, 2018
Executive summary: Diagnosis and Treatment of Catheter-Related Bloodstream Infection: Clinical Guidelines of the Spanish Society of Clinical Microbiology and Infectious Diseases (SEIMC) and the Spanish Society of Intensive Care Medicine and Coronary Units (SEMICYUC)
Fernando Chaves, José Garnacho-Montero, José Luis del Pozo, Emilio Bouza, José Antonio Capdevila, et al.
Enfermedades Infecciosas Y Microbiologia Clinica, 2018
Activity of maltodextrin and vancomycin against staphylococcus aureus biofilm
Maria Guembe
Frontiers in Bioscience Scholar, 2018
Diagnosis and treatment of catheter-related bloodstream infection: Clinical guidelines of the Spanish Society of Infectious Diseases and Clinical Microbiology and (SEIMC) and the Spanish Society of Spanish Society of Intensive and Critical Care Medicine and Coronary Units (SEMICYUC)
F. Chaves, J. Garnacho-Montero, J.L. del Pozo, E. Bouza, J.A. Capdevila, et al.
Medicina Intensiva, 2018
Eradication of P. aeruginosa biofilm in endotracheal tubes based on lock therapy: Results from an in vitro study
María Jesús Pérez-Granda, María Consuelo Latorre, Beatriz Alonso, Javier Hortal, Rafael Samaniego, et al.
BMC Infectious Diseases, 2017
Nationwide study on peripheral-venous-catheter-associated-bloodstream infections in internal medicine departments
M. Guembe, M.J. Pérez-Granda, J.A. Capdevila, J. Barberán, B. Pinilla, et al.
Journal of Hospital Infection, 2017
Should non-bacteraemic patients with a colonized catheter receive antimicrobial therapy?
V. De Egea, M. Guembe, A. Rodríguez-Borlado, M.J. Pérez-Granda, C. Sánchez-Carrillo, et al.
International Journal of Infectious Diseases, 2017
Comparison of the XTT and resazurin assays for quantification of the metabolic activity of Staphylococcus aureus biofilm
Beatriz Alonso, Raquel Cruces, Adrián Pérez, Carlos Sánchez-Carrillo, María Guembe
Journal of Microbiological Methods, 2017
Slicing silicone neonatal vascular catheter tips improves colonization detection by the roll-plate technique
M. Guembe, P. Martín-Rabadán, R. Cruces, M.J. Pérez Granda, E. Bouza
Clinical Microbiology and Infection, 2017
Improved method for the detection of catheter colonization and catheter-related bacteremia in newborns
P. Martín-Rabadán, F. Pérez-García, E. Zamora Flores, E.S. Nisa, M. Guembe, et al.
Diagnostic Microbiology and Infectious Disease, 2017
In Reply: Roll-plate Alone Does Demonstrate Colonization in Silicone Neonatal Catheters
María Guembe, Pablo Martín-Rabadán, Pablo Martín-Rabadán, Raquel Cruces, María Jesús Pérez Granda, et al.
Pediatric Infectious Disease Journal, 2017
The neutraclear® needleless connector is equally effective against catheter colonization compared to microclave®
María Guembe, María Jesús Pérez Granda, Raquel Cruces, Luis Alcalá, Emilio Bouza
Journal of Vascular Access, 2017
Assessment of biofilm production in Candida isolates according to species and origin of infection
María Guembe, Raquel Cruces, Teresa Peláez, Patricia Muñoz, Emilio Bouza
Enfermedades Infecciosas Y Microbiologia Clinica, 2017
Does biomass production correlate with metabolic activity in Staphylococcus aureus?
Beatriz Alonso, José Lucio, María Jesús Pérez-Granda, Raquel Cruces, Carlos Sánchez-Carrillo, et al.
Journal of Microbiological Methods, 2016
Erratum: Corrigendum to “sonicating multi-lumen sliced catheter tips after the roll-plate technique improves the detection of catheter colonization in adults” (Journal of Microbiological Methods (2016) 122 (20–22)(S0167701216300045)(10.1016/j.mimet.2016.01.004))
María Guembe, Pablo Martín-Rabadán, Raquel Cruces, María Jesús Pérez Granda, Emilio Bouza
Journal of Microbiological Methods, 2016
Erratum: Roll-Plate Alone Does Not Demonstrate Colonization in Silicone Neonatal Catheters (The Pediatric Infectious Disease Journal (2016) 35 (351-353) DOI: 10.1097/INF.0000000000001036)
Pediatric Infectious Disease Journal, 2016
Use of MALDI-TOF to detect colonized vascular catheter tips after 6 and 12 h of incubation
Emilio Bouza, Raquel Cruces, María Jesús Pérez Granda, Belén Rodríguez-Sánchez, Pablo Martín-Rabadán, et al.
Journal of Microbiological Methods, 2016
Erratum: Corrigendum to “Sonicating multi-lumen sliced catheter tips after the roll-plate technique improves the detection of catheter colonization in adults” (Journal of Microbiological Methods (2016) 122 (20–22) (S0167701216300045) (10.1016/j.mimet.2016.01.004))
María Guembe, Pablo Martín-Rabadán, Raquel Cruces, María Jesús Pérez-Granda, Emilio Bouza
Journal of Microbiological Methods, 2016
The tego™ needleless connector for hemodialysis catheters may protect against catheter colonization
M. Guembe, M. J. Pérez-Granda, R. Cruces, L. Alcalá, E. Bouza
European Journal of Clinical Microbiology and Infectious Diseases, 2016
2016 Expert consensus document on prevention, diagnosis and treatment of short-term peripheral venous catheter-related infections in adult
Revista Espanola De Quimioterapia Publicacion Oficial De La Sociedad Espanola De Quimioterapia, 2016
Erratum: Cultures of needleless connectors are useful for ruling out central venous catheter colonization (Journal of Clinical Microbiology (2015) 53:7 (2068-2071))
María Guembe, María Jesús Pérez-Granda, Raquel Cruces, Pablo Martín-Rabadán, Emilio Bouza
Journal of Clinical Microbiology, 2016
2016 Expert consensus document on prevention, diagnosis and treatment of short-term peripheral venous catheter-related infections in adults
Josep A. Capdevila, María Guembe, José Barberán, Arístides de Alarcón, Emilio Bouza, et al.
Cirugia Cardiovascular, 2016
Erratum to "Effectiveness of a training program in compliance with recommendations for venous lines care" [BMC Infect Dis. (2015), 15, 296.]
M. J. Pérez-Granda, M. Guembe, C. Rincón, P. Muñoz, E. Bouza
BMC Infectious Diseases, 2016
Sonicating multi-lumen sliced catheter tips after the roll-plate technique improves the detection of catheter colonization in adults
María Guembe, Pablo Martín-Rabadán, Raquel Cruces, María Jesús Pérez Granda, Emilio Bouza
Journal of Microbiological Methods, 2016
Roll-plate alone does not demonstrate colonization in silicone neonatal catheters
María Guembe, Pablo Martín-Rabadán, Raquel Cruces, María Jesús Pérez Granda, Emilio Bouza
Pediatric Infectious Disease Journal, 2016
Assessment of central venous catheter colonization using surveillance culture of withdrawn connectors and insertion site skin
María Jesús Pérez-Granda, María Guembe, Raquel Cruces, José María Barrio, Emilio Bouza
Critical Care, 2016
Vascular catheter colonization: Surveillance based on culture of needleless connectors
María Jesús Pérez-Granda, María Guembe, Raquel Cruces, Emilio Bouza
Critical Care, 2016
MALDI-TOF is not useful in the diagnosis of catheter colonization based on superficial cultures: Results from an in vitro study
María Guembe, María Jesús Pérez-Granda, Raquel Cruces, Belén Rodríguez-Sánchez, Adrián Ruíz, et al.
Diagnostic Microbiology and Infectious Disease, 2016
Stickers used for the identification of intravenous lines could be a portal of entry of microorganisms through the catheter: Results from a clinical study
María Jesús Pérez Granda, Raquel Cruces, Pablo Martín-Rabadán, Emilio Bouza, María Guembe
American Journal of Infection Control, 2015
Effectiveness of a training program in compliance with recommendations for venous lines care
M. J. Pérez-Granda, M. Guembe, C. Rincón, P. Muñoz, E. Bouza
BMC Infectious Diseases, 2015
Cultures of needleless connectors are useful for ruling out central venous catheter colonization
María Guembe, María Jesús Pérez-Granda, Raquel Cruces, Pablo Martín-Rabadán, Emilio Bouza
Journal of Clinical Microbiology, 2015
Performing Gram stain directly on catheter tips: assessment of the quality of the observation process
M. Guembe, M. J. Pérez-Granda, M. L. Rivera, P. Martín-Rabadán, E. Bouza
European Journal of Clinical Microbiology and Infectious Diseases, 2015
Nationwide study on the use of intravascular catheters in internal medicine departments
M. Guembe, M.J. Pérez-Granda, J.A. Capdevila, J. Barberán, B. Pinilla, et al.
Journal of Hospital Infection, 2015
Stickers used for identification of intravenous lines may be a source of contamination
María Guembe, María Jesús Pérez Granda, Raquel Cruces, Pablo Martín-Rabadán, Emilio Bouza
American Journal of Infection Control, 2015
The role of antifungals against Candida biofilm in catheter-related candidemia
Emilio Bouza, Jesús Guinea, María Guembe
Antibiotics, 2014
A simple and easy in vitro model to test the efficacy of IV lines' needleless connectors against contamination
María Guembe, María Jesús Pérez-Granda, Luis Alcalá, Pablo Martín-Rabadán, Emilio Bouza
Intensive Care Medicine Experimental, 2014
Predictive value of superficial cultures to anticipate tunneled hemodialysis catheter-related bloodstream infection
Emilio Bouza, Loreto Rojas, María Guembe, Mercedes Marín, Fernando Anaya, et al.
Diagnostic Microbiology and Infectious Disease, 2014
Diagnosis of venous access port colonization requires cultures from multiple sites: Should guidelines be amended?
Emilio Bouza, Pablo Martín-Rabadán, Antonio Echenagusia, Fernando Camúñez, Gracia Rodríguez-Rosales, et al.
Diagnostic Microbiology and Infectious Disease, 2014
Colonization of stickers used for the identification of intravenous lines: Results from an in vitro study
María Jesús Pérez Granda, María Guembe, Raquel Cruces, Pablo Martín-Rabadán, Emilio Bouza
American Journal of Infection Control, 2014
A prevalence survey of intravascular catheter use in a general hospital
María Jesús Pérez-Granda, María Guembe, Cristina Rincón, Patricia Muñoz, Emilio Bouza
Journal of Vascular Access, 2014
Can MALDI-TOF mass spectrometry be used with intravascular catheters?
María Guembe, Belén Rodríguez-Sánchez, Adrián Ruiz, Pablo Martín-Rabadán, Marta Rodríguez-Créixems, et al.
Enfermedades Infecciosas Y Microbiologia Clinica, 2014
The risk of catheter-related bloodstream infection after withdrawal of colonized catheters is low
M. Guembe, M. Rodríguez-Créixems, P. Martín-Rabadán, L. Alcalá, P. Muñoz, et al.
European Journal of Clinical Microbiology and Infectious Diseases, 2014
Is Biofilm Production a Predictor of Catheter-Related Candidemia?
M. Guembe, J. Guinea, L. Marcos-Zambrano, A. Fernandez-Cruz, T. Pelaez, et al.
Medical Mycology, 2014
Micafungin at physiological serum concentrations shows antifungal activity against Candida albicans and Candida parapsilosis biofilms
M. Guembe, J. Guinea, L. J. Marcos-Zambrano, A. Fernández-Cruz, T. Peláez, et al.
Antimicrobial Agents and Chemotherapy, 2014
Is it feasible to diagnose catheter-related candidemia without catheter withdrawal?
A. Fernandez-Cruz, P. Martin-Rabadan, M. Suarez-Salas, L. Rojas-Wettig, M. J. Perez, et al.
Medical Mycology, 2014
Bloodstream infections in patients with kidney disease: Risk factors for poor outcome and mortality
L. Rojas, P. Muñoz, M. Kestler, D. Arroyo, M. Guembe, et al.
Journal of Hospital Infection, 2013
Value of superficial cultures for prediction of catheter-related bloodstream infection in long-term catheters: A prospective study
M. Guembe, P. Martín-Rabadán, A. Echenagusia, F. Camúñez, G. Rodríguez-Rosales, et al.
Journal of Clinical Microbiology, 2013
Use of universal 16s RRNA gene PCR as a diagnostic tool for venous access port-related bloodstream infections
M. Guembe, M. Marín, P. Martín-Rabadán, A. Echenagusia, F. Camúñez, et al.
Journal of Clinical Microbiology, 2013
Catheter-related bloodstream infection caused by Enterococcus spp.
E. Reigadas, M. Rodríguez-Creixems, M. Guembe, C. Sanchez-Carrillo, P. Martín-Rabadán, et al.
Clinical Microbiology and Infection, 2013
Evolution and aetiological shift of catheter-related bloodstream infection in a whole institution: The microbiology department may act as a watchtower
M. Rodríguez-Créixems, P. Muñoz, P. Martín-Rabadán, E. Cercenado, M. Guembe, et al.
Clinical Microbiology and Infection, 2013
Can microbiologists help to assess catheter involvement in candidaemic patients before removal?
E. Bouza, On behalf of the GEIDI and the COMIC study groups, L. Alcalá, P. Muñoz, P. Martín-Rabadán, et al.
Clinical Microbiology and Infection, 2013
Impact on knowledge and practice of an intervention to control catheter infection in the ICU
on behalf of the GEIDI Study Group, M. Guembe, A. Pérez-Parra, E. Gómez, M. Sánchez-Luna, et al.
European Journal of Clinical Microbiology and Infectious Diseases, 2012
Differential time to positivity (DTTP) for the diagnosis of catheter-related bloodstream infection: Do we need to obtain one or more peripheral vein blood cultures?
M. Guembe, M. Rodríguez-Créixems, C. Sánchez-Carrillo, P. Martín-Rabadán, E. Bouza
European Journal of Clinical Microbiology and Infectious Diseases, 2012
Guidelines for preventing catheter infection: Assessment of knowledge and practice among paediatric and neonatal intensive care healthcare workers
M. Guembe, A. Bustinza, M. Sánchez Luna, A. Carrillo-Álvarez, V. Pérez Sheriff, et al.
Journal of Hospital Infection, 2012
How should long-term tunneled central venous catheters be managed in microbiology laboratories in order to provide an accurate diagnosis of colonization?
M. Guembe, P. Martín-Rabadán, A. Echenagusia, F. Camúñez, G. Rodríguez-Rosales, et al.
Journal of Clinical Microbiology, 2012
Central venous catheter colonization with Staphylococcus aureus is not always an indication for antimicrobial therapy
P. Muñoz, A. Fernández Cruz, R. Usubillaga, A. Zorzano, M. Rodríguez-Créixems, et al.
Clinical Microbiology and Infection, 2012
Managing intravascular catheter-related infections in heart transplant patients: How far can we apply IDSA guidelines for immunocompromised patients?
Emilio Bouza, Almudena Burillo, María Guembe
Current Opinion in Infectious Diseases, 2011
Corrigendum to "Prospective, randomised study of selective versus routine culture of vascular catheter tips: Patient outcome, antibiotic use and laboratory workload" [Journal of Hospital Infection 2011;77:309-315]
A. Pérez-Parra, M. Guembe, P. Martín-Rabadán, P. Muñoz, A. Fernández-Cruz, et al.
Journal of Hospital Infection, 2011
Prospective, randomised study of selective versus routine culture of vascular catheter tips: Patient outcome, antibiotic use and laboratory workload
A. Pérez-Parra, M. Guembe, P. Martín-Rabadán, P. Muñoz, A. Fernández-Cruz, et al.
Journal of Hospital Infection, 2011
Selection of the vascular catheter: Can it minimise the risk of infection?
E. Bouza, M. Guembe, P. Muñoz
International Journal of Antimicrobial Agents, 2010
How many lumens should be cultured in the conservative diagnosis of catheter-related bloodstream infections?
María Guembe, Marta Rodríguez‐Créixems, Carlos Sánchez‐Carrillo, Alfonso Pérez‐Parra, Pablo Martín‐Rabadán, et al.
Clinical Infectious Diseases, 2010
Are central venous catheter tip cultures reliable after 6-day refrigeration?
Emilio Bouza, Maria Guembe, Haydee Gómez, Pablo Martín-Rabadán, Marisa Rivera, et al.
Diagnostic Microbiology and Infectious Disease, 2009
Is Candida colonization of central vascular catheters in non-candidemic, non-neutropenic patients an indication for antifungals?
Alfonso Pérez-Parra, Patricia Muñoz, Jesús Guinea, Pablo Martín-Rabadán, María Guembe, et al.
Intensive Care Medicine, 2009
Evolution of antimicrobial susceptibility patterns of aerobic and facultative gram-negative bacilli causing intra-abdominal infections: Results from the SMART studies 2003-2007
Revista Espanola De Quimioterapia, 2008
Synergistic effect of posaconazole and caspofungin against clinical zygomycetes [2]
María Guembe, Jesús Guinea, Teresa Peláez, Marta Torres-Narbona, Emilio Bouza
Antimicrobial Agents and Chemotherapy, 2007

María Guembe

RESEARCH, TEACHING, or OTHER INTERESTS

Scopus Publications