Lais Duarte Batista

Scopus Publications

Alpha-tocopherol intake and status exhibit population-specific associations with metabolic syndrome among two Hispanic/Latinos populations
Marcela Larissa Costa, Josiemer Mattei, Lais Duarte Batista, Cristiane Hermes Sales, Flávia Mori Sarti, Marcelo Macedo Rogero, Regina Mara Fisberg
Nutrition Research, 2026
Adequate alpha-tocopherol intake and status may reduce the risk of metabolic syndrome (MetS), yet evidence among Hispanics/Latinos remains limited. This study examined population-specific associations of alpha-tocopherol intake and status with MetS in two Hispanic/Latino populations and identified the main dietary sources of alpha-tocopherol. We hypothesized that alpha-tocopherol intake and status would be inversely associated with MetS and its components, with distinct food sources across populations. Cross-sectional analysis was conducted from the 2015 Health Survey of São Paulo with a Focus on Nutrition (n = 524) and Hispanic/Latino respondents of the 2017 to 2018 National Health and Nutrition Examination Survey (n = 499). Alpha-tocopherol intake was assessed from two 24-hour recalls, and status from blood plasma samples. MetS was classified based on International Diabetes Federation criteria using standardized laboratory and clinical measures. Poisson regression models considered complex survey design. Alpha-tocopherol intake and status were inversely associated with higher triglycerides and MetS in the São Paulo population. Only alpha-tocopherol status was associated with higher fasting glycemia, higher triglycerides, lower HDL-c, higher waist circumference, and MetS in Hispanic/Latino individuals from the US. The food sources of alpha-tocopherol varied substantially by population. In conclusion, alpha-tocopherol intake and status were associated differently with metabolic syndrome components according to the population, underscoring the need for tailored strategies to improve alpha-tocopherol adequacy for cardiometabolic health.
FADS1 and FADS2 Gene Polymorphisms Affect Omega-3 and Omega-6 Erythrocyte Fatty Acid Composition and Influence the Association Between Dietary Fatty Acid Intake and Lipid Profile in Brazilian Adults
Lais Duarte Batista, Marcelo Macedo Rogero, Flávia Mori Sarti, Marcela Larissa Costa, Jaqueline Lopes Pereira França, João Valentini Neto, Regina Mara Fisberg
Metabolites, 2025
Background: Polymorphisms in the FADS1 and FADS2 genes influence fatty acid metabolism. However, evidence of gene–diet interactions in population-based studies from Brazil remains limited. The objective of this study was to examine associations between FADS1–FADS2 single-nucleotide polymorphisms (SNPs) and erythrocyte fatty acid composition and serum lipid concentrations, as well as to explore potential gene–diet interactions. Methods: Data were analyzed from 294 adults (20–93 years) enrolled in the 2015 ISA-Nutrition study. Erythrocyte fatty acid composition and serum lipids were measured using standard enzymatic methods. Dietary intake was assessed by 24 h recalls, and participants were classified into tertiles according to fatty acid intake. Five SNPs were genotyped; FADS1 rs174546 and FADS2 rs174570 were prioritized based on linkage disequilibrium. Associations and interactions were assessed using generalized linear models, adjusting for confounders. Results: Carriers of the minor alleles for rs174546 and rs174570 had significantly lower erythrocyte levels of long-chain polyunsaturated fatty acids, particularly along the ω-6 pathway, suggesting reduced desaturase activity. The rs174546 TT genotype was associated with higher total, very-low-density lipoprotein cholesterol (VLDL), and non–high-density lipoprotein (non-HDL) cholesterolconcentrations. Higher dietary intakes of docosahexaenoic acid (DHA) or a higher linoleic acid to alpha-linolenic acid ratio(LA/ALA ratio) among these carriers were linked to lower serum lipid levels, indicating gene–diet interactions that attenuate adverse genotype effects. In addition, rs174570 TT carriers showed elevated VLDL concentrations, with a significant dietary interaction observed with the LA/ALA ratio. Conclusions: FADS1 and FADS2 polymorphisms influence fatty acid metabolism and interact with diet to shape lipid profiles. These findings highlight the importance of considering gene-diet interactions in cardiometabolic risk.
Association of two cut-off points for the n-3 index with cardiometabolic risk factors in Brazilian and Puerto Rican middle-aged adults
Lais Duarte Batista, Katherine L. Tucker, Sherman Bigornia, Sabrina E. Noel, William S. Harris, Ribanna A. Marques Braga, João Valentini Neto, Nágila R. Teixeira Damasceno, Marcelo Macedo Rogero, Flavia Mori Sarti, Josiemer Mattei, Regina M. Fisberg
British Journal of Nutrition, 2025
The n-3 index has been proposed as a risk factor for CVD endpoints. However, the association of the O3I defined with different cut-offs and cardiometabolic risk factors has been less studied. This study aimed to investigate the association between two cut-off points of the O3I and cardiometabolic risk factors in Brazilian and Puerto Rican adults. This cross-sectional analysis included 249 Brazilians and 1261 Puerto Ricans, aged 45–75 years. Fatty acids composition was quantified in erythrocyte membranes using GC with a flame ionisation detector. The O3I was categorised as ≤ 4 % (low), > 4–8 % (intermediate) and ≥ 8 % (desirable), and as ≤ 4 % (very low), > 4–6 % (low), > 6–8 % (moderate) and > 8 % (high) in the second cut-off classification. Serum lipids, waist circumference and insulin resistance were measured from standardised protocols. Multivariable-adjusted linear models tested the association between the O3I and cardiometabolic factors. Brazilians had a mean (sd) O3I of 4·65 % (1·19 %) v. 4·43 % (1·14 %) in Puerto Ricans (P = 0·033), with only 1·6 % of Brazilians and 1·2 % of Puerto Ricans presenting a desirable/high O3I. The O3I, as continuous or for > 4 % (v. ≤ 4 %), was inversely associated with TAG, VLDL and TAG/HDL-cholesterol ratio in Puerto Ricans. In Brazilians, an O3I > 6 % (v. ≤ 6 %) was associated with higher total cholesterol, LDL-cholesterol and non-HDL-cholesterol. Both populations presented O3I below the desirable levels, and the magnitude and direction of associations with cardiometabolic factors varied by study and cut-offs, reinforcing the importance of expanding these investigations to more diverse populations.
Pragmatic Carbohydrate Quality Metrics in Relation to Glycemic Index, Glycemic Load, and Front-of-Pack Warning Labels in Grain Foods
Mariane de Mello Fontanelli, Lais Duarte Batista, Angela Martinez-Arroyo, Dariush Mozaffarian, Renata Micha, Marcelo Macedo Rogero, Regina Mara Fisberg, Flavia Mori Sarti
Foods, 2024
The challenges in the characterization of the nutritional quality of grain foods comprise obstacles to public health actions toward promotion of healthier grain-based foods. The present study investigated how carbohydrate metrics related to glycemic index (GI), glycemic load (GL), and warning labels of grain foods consumed by individuals living in São Paulo, Brazil. Information on intake of grain foods at individual level was obtained using 24 h recalls within a cross-sectional population-based survey conducted in 2015. There were 244 unique grain products reported by individuals in the survey, assessed through four metrics of carbohydrate quality, considering contents per 10 g of total carbohydrate: (1) ≥1 g fiber, (2) ≥1 g fiber and <1 g free sugars, (3) ≥1 g fiber and <2 g free sugars, and (4) ≥1 g fiber, and <2 g free sugars per 1 g of fiber. Outcomes included GI, GL, and inclusion of warning labels proposed by the Brazilian National Health Surveillance Agency (ANVISA), the Chilean Ministry of Health (1st and 3rd stages), and the Pan American Health Organization (PAHO). Metrics identified products with lower mean GI (−12.8 to −9.0 [p-values < 0.001]), and GL (−12.5 to −10.3 [p-values < 0.001]). Warning systems showed a certain degree of discrimination between products according to the metrics (p-value < 0.01 each); however, >50% of products with good nutritional quality according to the carbohydrate metrics still would receive warnings. Findings suggest that carbohydrate metrics identified products with lower GI and GL, and current warning labels may not adequately capture overall nutritional quality of grain foods.
Exploring Diet and Nutrient Insufficiencies across Age Groups: Insights from a Population-Based Study of Brazilian Adults
Mauro Fisberg, Lais Duarte Batista, Agatha Nogueira Previdelli, Gerson Ferrari, Regina Mara Fisberg
Nutrients, 2024
Assessing dietary inadequacies can contribute to understanding the nutritional vulnerabilities of a country. This study aimed to investigate nutrient intakes and micronutrient inadequacies in the Brazilian adult population, with an interest in different age subgroups. We conducted a cross-sectional study with 1812 individuals aged 19 to 65 years from a population-based study with a representative sample of Brazilian adults. Dietary intake was assessed by two 24 h food recalls, and the probabilities of inadequate intake were estimated using the Dietary Reference Intake targets. Adequate macronutrient intake was over 99% for proteins, 84.7% for carbohydrates, and 80.7% for total fats. There was a high probability of inadequacy (above 90%) for vitamins D and E, but vitamin D inadequacy was very similar between the sexes. In contrast, vitamin E was more likely to be inadequately consumed among women. A high probability of inadequacies (above 85%) of calcium and magnesium were found in the population, regardless of age group. Except for iron, the probability of an inadequacy of other minerals increased with age. The results showed a relevant proportion of nutrient inadequacies, with those most at risk being women and older individuals, helping with the better targeting and monitoring of public-health policies that address nutritional problems in the population.
Body composition affects the accuracy of predictive equations to estimate resting energy expenditure in older adults: An exploratory study
Lais Duarte Batista, João Valentini Neto, Natasha Aparecida Grande de França, Sandra Maria Lima Ribeiro, Regina Mara Fisberg
Clinical Nutrition Espen, 2023
Misreporting of dietary energy intake obtained by 24 h recalls in older adults: a comparison of five previous methods using doubly labeled water
Lais Duarte Batista, Natasha Aparecida Grande de França, Mariane de Mello Fontanelli, Angela Graciela Martinez-Arroyo, Regina Mara Fisberg
European Journal of Clinical Nutrition, 2022
To test five different methods to detect misreporting in comparison to doubly labeled water in a sample of older adults. A cross-sectional study with thirty-eight Brazilian community-dwelling older adults aged 60–84 years, who had their total energy expenditure measured by doubly labeled water (TEEDLW). Dietary data were collected by two 24 h recalls. Misreporting was compared with estimates obtained by the methods proposed by: Goldberg et al. [1, 2], Black [3], McCrory et al. [4], Huang et al [5], and Rennie et al [6]. Bland–Altman plots with 95% limits of agreement were constructed to assess the agreement between rEI and TEEDLW. Weighted kappa coefficients, sensitivity and specificity analyses, and area under the receiving operator characteristic curve (AUC) were used to test the performance of each method. The prevalence of under-reporters (UR) and over-reporters (OR) obtained by the reference (DLW) were 57.9% (n = 22) and 5.3% (n = 2), respectively. Black [3] presented the worst agreement and McCrory et al. [4] the best one to accurately classify individuals in the three categories of energy reporting. McCrory et al. [4] had the best performance in the sensitivity and specificity analyses detecting UR and plausible reporters. There was a high prevalence of misreporting, especially underreporting, in this sample of community-dwelling Brazilian older adults. The study showed a wide variation in the accuracy of predictive methods to handle misreporting, with none of the equations showing outstanding agreement with the reference. When DLW is not available, a valid method should be chosen to address energy intake reporting.
Misreporting of Energy Intake Is Related to Specific Food Items in Low-Middle Income Chilean Adolescents
Angela Martínez-Arroyo, Lais Duarte Batista, Camila Corvalán Aguilar, Regina Mara Fisberg
Children, 2022
Background: Misreporting of energy intake (EI) in self-reported dietary assessment is inevitable, and even less is known about which food items are misreported by low-middle income adolescents. We evaluated the prevalence of misreporting of energy intake and its relationship with nutrients and food intake. Methods: We analyzed 24 h dietary recalls collected from 576 adolescents (52.08% boys) from southeastern Santiago. Anthropometrics measurements and information about sociodemographic characteristics were obtained during clinical visits. The method proposed by McCrory et al. was used to identify under-reporters (UnRs), over-reporters (OvRs), or plausible reporters (PRs). Food items were collapsed into 28 categories and every food item was expressed as a percentage of total EI. Logistic regression models were performed to investigate the factors associated with misreporting, and a two-part model was used to estimate the difference in the percentage of EI between UnRs versus PRs, and OvRs versus PRs in each food item. Results: Half of the participants were classified as UnRs and 9% were OvRs. UnR was higher among boys (62%) and adolescents with overweight and obesity (72%). OvR was higher among adolescents with normal weight. UnRs had a lower intake of energy from cookies/cake, chocolate/confectionery, and a higher intake of vegetables and eggs than PRs. OvRs had a higher intake of cookies/cake, chocolate/confectionery, and a lower intake of fruit, white milk, and yogurt than PRs. Conclusions: A high frequency of UnR among boys and participants with excess weight was found in this study. Healthy and unhealthy foods are reported differently between UnRs and OvRs of energy intake, indicating that bias is specific for some food items that adolescents commonly eat.
Integrative Strategies for Preventing Nutritional Problems in the Development of Children in Brazil
Mauro Fisberg, Lais Duarte Batista, Carlos Alberto Nogueira-de-Almeida, Flávia Mori Sarti, Maria Paula de Albuquerque, Regina Mara Fisberg
Frontiers in Nutrition, 2021
Child healthcare has been a priority subject in several programs and public policies developed over the decades. However, initiatives implemented seem insufficient to overcome the challenges regarding the integral development and improvement of the nutritional status of children in Brazil. The initial developmental stages of a child include pregnancy, breastfeeding, and complementary feeding, which are determinants in future aspects of health and nutritional status. Therefore, the strategies addressing problems during these three periods of life have the potential to positively impact the promotion of healthy eating habits and food security throughout life. Developing countries with huge dimensions and vast inequalities, like Brazil, are marked by differences in regional, cultural, and social contexts that may hinder the implementation of programs and policies with a broad scope. Extensive operational and professional costs, in addition to time-consuming activities that are necessary to apply, monitor, and evaluate interventions may jeopardize the proper assessment of programs and policy goals, generating the inefficiency and waste of resources in the health system. Thus, programs and policies aimed at creating and modifying habits should consider an intersectoral action within local contexts, involving health professionals, universities, policy managers, and the community. Therefore, this article aimed to discuss the preliminary conception of an integrated approach of decentralized strategies to promote healthy eating habits and food security of children in Brazil.
Estimating total daily energy requirements in community-dwelling older adults: validity of previous predictive equations and modeling of a new approach
Lais Duarte Batista, Natasha Aparecida Grande De França, Karina Pfrimer, Mariane de Mello Fontanelli, Eduardo Ferriolli, Regina Mara Fisberg
European Journal of Clinical Nutrition, 2021
Background/objectives Accurate estimation of energy requirements is crucial for health maintenance and prevention of malnutrition in older adults. This study aimed to assess the accuracy of predictive equations for estimating energy requirements in older adults and to test the validity of new predictive equations for this age group. Subjects/methods This is a cross-sectional study including 38 Brazilian community-dwelling older adults aged 60–84 years, who had their total energy expenditure measured by doubly labeled water (TEE DLW ). The energy expenditure was compared to the Institute of Medicine (Dietary Reference Intake (DRI)) and Vinken et al. previous predictive equations and three predictive models developed in a modeling sample. The agreement was assessed using intra-class correlation coefficient, Bland–Altman plots, and Lin’s concordance correlation. Accuracy was evaluated considering ±10% of the ratio between estimated and measured energy expenditure. Results The mean (standard deviation) TEE DLW was 2656.7 (405.6) kcal/day for men and 2168.9 (376.9) for women. Vinken et al. and both DRI equations presented moderate to good degree of agreement, while the developed models vary from fair to very good agreement in comparison to DLW. The accuracy rate was the same for both DRI equations and Vinken et al. equation (60.53%). The new equations developed in this study had accuracy in predicting TEE for Brazilian older adults varying from 43.11% to 73.68%. Conclusions The results corroborate the use of previous predictive equations for estimating energy requirements in Brazilian older adults. Further studies have the potential to explore the use of the developed models to assess energy needs in this population.
Nutrition Related-Practices in Brazilian Preschoolers: Identifying Challenges and Addressing Barriers
Mauro Fisberg, Lais Duarte Batista
Nestle Nutrition Institute Workshop Series, 2020
Increased sugar-sweetened beverage consumption is associated with poorer dietary quality: A cross-sectional population-based study
Amanda Silva FONTES, Ana Carolina PALLOTTINI, Diva Aliete dos Santos VIEIRA, Lais Duarte BATISTA, Mariane de Mello FONTANELLI, Regina Mara FISBERG
Revista De Nutricao, 2019