Lalit Kumar Singh
@hbtu.ac.in
Professor, Department of Biochemical Engineering
Harcourt Butler Technical University Kanpur
EDUCATION
B. Tech.
M. Tech.
Ph.D.
RESEARCH, TEACHING, or OTHER INTERESTS
Renewable Energy, Sustainability and the Environment, Biotechnology, Process Chemistry and Technology, Waste Management and Disposal
Scopus Publications
Scopus Publications
Tirath Raj, Sandhya Sompura, K. Chandrasekhar, Sushil Kumar Singh, Srinath Pandey, Lalit Kumar Singh, Manish Singh Rajput, Deepak Kumar, Shashi Kant Bhatia, Anil Kumar Patel,et al.
Elsevier BV
LalitKumar Singh and Zainab Mahmood
Medknow
Background: The promising use of lignocellulosic materials as a substrate for the synthesis of biochemical and biofuels has long been recognized. The bioconversion of all lignocellulosic sugars into useful products results in high sugar yields, which makes lignocelluloses appealing for use. Pretreatment and hydrolysis of lignocelluloses at high solids loadings are vital throughout industrial processes, making them more cost-effective and ecologically friendly by lowering the need for capital expenditures, energy use, and water use. The high expense of fermentation medium hinders heterotrophic oleaginous microorganisms' ability to metabolically synthesize single-cell oils for biodiesel use. This study's objective was to ascertain whether bagasse hydrolysate could be used as a cost-effective feedstock for oleaginous Rhodococcus opacus to produce microbial lipids. Methods: R. opacus a strain was used in this study. The culture was first of all maintained at soytone nutrient growth medium (i.e. GROTH MEDIUM 56) and subcultured at regular intervals and as well as preserved at 4°C. For the production media, a minimal salt medium is used. Sugarcane bagasse is pretreated by acidic hydrolysis followed by detoxification of hydrolysate and lipid extraction is done by Folch's extraction process. Results: After a brief adaptation/lag phase, the strain showed a viable growth pattern once the nutrients were detoxicated through the removal of inhibitors (e.g. hydroxymethyl-furfural [HMF]). R. opacus was grown via shake flask fermentation in bagasse hydrolysate with different sugar concentrations. When cultivated in S2 experiment, R. opacus obtained over 50% of its dry cell weight as a lipid with a lipid yield of 64.47% and productivity is 0.015 g/L/h. Conclusions: Depending on the available substrates, different microbial oils had different compositions. These findings suggest that lignocellulosic hydrolysate can be utilized as an inexpensive fermentation substrate for the R. opacus to produce microbial lipids. As a result, the range of uses for aqueous liquors produced by lignocellulosic pretreatment has increased, enabling further improvement.
Ashutosh Kumar Pandey, Jungsu Park, Jeun Ko, Hwan-Hong Joo, Tirath Raj, Lalit Kumar Singh, Noopur Singh, and Sang-Hyoun Kim
Elsevier BV
LalitKumar Singh and Abhimati Shukla
Medknow
Background: The wastewater disposal into the water bodies without removing the toxic heavy metals and other industrial impurities is a major issue these days. These heavy metals cause serious health issues to the human and animal life and also harm the environment and reduce the productivity of crops. A potent microorganism resistant to hexavalent chromium was isolated. The activity of this isolated strain was analyzed using in silico studies. Methods: In this study, a chromium-resistant fungus was isolated from the soil of the dumping sites of the tanneries in Kanpur, UP, India, followed by isolation by serial dilution, plating method, and finally, genome sequencing. It was identified as Aspergillus proliferans LA that is submitted to the National Collection for Industrial Microorganisms (NCIM) database with accession no. NCIM-1473. In the current study, the comparative analysis of the protein sequence of A. proliferans (NCIM-1473) was done against the known 53 protein sequences of the fungus and bacterial strains already reported for their chromium-resistant nature. The physical and chemical parameters of the known and isolated chromium-resistant proteins were analyzed using the ProtParam tool. The comparative study on the sequence and structural alignment of known and isolated chromium-resistant protein was done using EMBOSS-NEEDLE and FATCAT, respectively. Results: In this analysis, the top 10 strains showing similarity with A. proliferans (NCIM-1473) were reported and among which ChrI, chromium regulatory protein Cupriavidus metallidurans CH34 was showing maximum similarity with isolated chromium resistant protein for all the analysis, namely ProtParam, sequence, and FATCAT analysis. This strain, Cupriavidus metallidurans CH34, has been reported resistant against eight heavy metals, one of which is chromate, and was first identified in the heavy-metal contaminated sludge in a settling tank of Belgium. Conclusion: These studies conclude that the strain isolated in our laboratory (accession no. NCIM-1473) is potentially chromium resistant and a unique strain.
Lalit Kumar Singh, Garima Awasthi, and Mangalam Bajpai
Springer Nature Singapore
Amrendra Pathak and Lalit Kumar Singh
Elsevier
Amrendra Nath Pathak and Lalit Kumar Singh
Elsevier
Anamika Kushwaha, Vivek Mishra, Veena Gupta, Shivani Goswami, Pankaj Kumar Gupta, Lalit Kumar Singh, Chandra Bhanu Gupt, Kaustubh Rakshit, and Lalit Goswami
Elsevier
Amrendra Nath Pathak, Lalit Kumar Singh, and Esha Dwivedi
Springer Singapore
Ashutosh Kumar Pandey, Ranjna Sirohi, Sudha Upadhyay, Mitali Mishra, Virendra Kumar, Lalit Kumar Singh, and Ashok Pandey
Elsevier
Ashutosh Kumar Pandey, Kritika Pandey, and Lalit Kumar Singh
Springer Singapore
L. Singh and G. Chaudhary
Wiley
L. Singh and G. Chaudhary
Wiley
Lalit K. Singh, Chandrajit B. Majumder, and Sanjoy Ghosh
Elsevier BV
Gaurav Chaudhary, Lalit Kumar Singh, and Sanjoy Ghosh
Elsevier BV
Lalit Kumar Singh, C. B. Majumder, and Sanjoy Ghosh
Informa UK Limited
Ethanol is a promising alternative energy source for the limited crude oil and can be produced from abundantly available lignocellulosic biomass. Utilization of acid hydrolysate of Kans grass (Saccharum spontaneum), a perennial grass native to South Asia, has been investigated for ethanol production. The dilute acid treatment was applied to hydrolyze the Kans grass biomass for releasing maximum hemicellulosic sugars. The aim of this work was to develop a fermentative system utilizing Kans grass hemicellulose acid hydrolysate as a substrate for ethanol production by Pichia stipitis. It was found that 74% of xylose was converted to ethanol with a yield of 0.429 gp gs −1 and productivity of 0.231 gp l−1 h−1. The appropriate mathematical models for cell and ethanol production rate have been developed to explain theoretically the bioconversion of Kans grass hemicellulose acid hydrolysate to ethanol and validated statistically with the experimental results carried out in the laboratory. The present study revealed that the degradation of sugar, activation and inhibition of cells, and ethanol production were strictly coupled during the bioconversion process of hemicellulosic fraction of Kans grass to ethanol.
Divya Gupta, Lalit Kumar Singh, Ashish Deep Gupta, and Vikash Babu
Wiley
Ashish Kumar, L.K. Singh, and Sanjoy Ghosh
Elsevier BV