Renewable Energy, Sustainability and the Environment, Biotechnology, Process Chemistry and Technology, Waste Management and Disposal
39
Scopus Publications
919
Scholar Citations
13
Scholar h-index
16
Scholar i10-index
Scopus Publications
Application of Biochar in Anaerobic Digestion Harshit Gupta, Prateek Mishra, Lalit Kumar Singh, Satyendra Prasad Chaurasia, Vivekanand Vivekanand Biochar and the Circular Economy Innovations in Production and Applications, 2026 The integration of biochar into anaerobic digestion (AD) systems has emerged as a transformative approach to enhance biogas production, improve process stability, and mitigate common inhibitory factors such as ammonia and volatile fatty acids. This chapter gives an overview of how biochars function in AD with respect to the digesters alone and not to include other applications such as soil amendment. It starts with the global context to have been given for why more bioenergy efficiency systems need to include biochar not just as a structural additive but also as an active biological and electrochemical catalyst, such as activation of direct inter-species electron transfer (DIET), pH buffering, and adsorption of toxins. Following that, it sets up a framework outlining the application of biochar in AD relative to the specific gases under concentration and how its activity is affected by physicochemical characteristics—surface area, porosity, functional groups, and pyrolysis specifications—and will include joint processes with different types of substrates (food wastes, manures, sewage sludges, and agricultural residues). Finally, some very interesting measurable parameters, such as increased yield of methane with lower lag phases, improved stabilization of VFAs, and a greater tolerance of very high organic loading rates, will be discussed, and all of these factors will be shown through batch, semi-continuous, and continuous AD systems. Critical aspects like dosage optimization, biochar leaching, and endurance under long-term conditions are reviewed as operational challenges followed by discussion on reuse and regeneration strategies. It flags off future research priorities such as developing smart biochars and engineering the microbial community to achieve better DIET with stringent techno-economic evaluation on a large scale. In sum, this synthesis sets the stage for a scalable, multifunctional role of biochar within anaerobic digestion toward a more metabolically resilient and circular “waste to energy” platform.
Application of Biochar in Biohydrogen Production Prateek Mishra, Sandhya Sompura, Maria Esther Medina Villafuerte, Jerry Luis Solis Valdivia, Lalit Kumar Singh Biochar and the Circular Economy Innovations in Production and Applications, 2026 This book chapter explores recent advancements in using biochar for hydrogen production. Biochar, a carbon-rich material, can be produced through various thermochemical processes such as pyrolysis, gasification, and hydrothermal carbonization. In recent years, it has gained attention as a promising catalyst for hydrogen generation due to its abundance, distinctive structure, and multifunctional properties. Its application as a catalyst can significantly boost hydrogen yield and quality, thanks to its high catalytic activity and thermal stability. The type of feedstock used to produce biochar also plays a key role in determining its physical and chemical characteristics. Moreover, its catalytic efficiency can be enhanced through treatments like alkali or acid activation, metal ion addition, incorporation of carbon-based materials, oxidation, gas purging, or steam treatment. This review provides a comprehensive overview of biochar's role, particularly as a catalyst and support in hydrogen production through dark fermentation and thermochemical processes. Overall, biochar catalysts offer strong potential for contributing to a more efficient and sustainable energy future.
Carbon quantum dots as a promising tool for heavy metal sensing and removal in wastewater Pooja Singh, Lalit Kumar Singh Desalination and Water Treatment, 2025 Heavy metal ions have attracted global attention owing to their significant contribution to environmental pollution, posing a potential hazard to human health that led to concern regarding the adverse impact of heavy metal contamination on well-being. While conventional water treatment technologies demonstrate effectiveness in certain scenarios, they face considerable challenges regarding economic viability, performance efficiency, and environmental impact, especially for trace contaminant removal in multi-pollutant systems. CQDs have emerged as a novel nanomaterial with exceptional optical, electronic, and adsorption properties that offer significant advantages. Carbon quantum dots (CQDs) have gained significant attention recentlyowing to their exceptional biocompatibility, water solubility, sensitivity, selectivity, ease of synthesis, cost-effectiveness, and environmental friendliness. These characteristics make CQDs an effective tool for severalecological applications, such as heavy metal detection, adsorption and dye degradation. The optical characteristics of green quantum dots may vary from those of metal-based quantum dots due to recent progress in synthesizing green quantum dots with favourable optical properties. Modifying the surface of quantum dots may increase their catalytic efficiency and durability. Enhancing the interactions between metal ions and quantum dots, together with improving the effectiveness of the photocatalytic process, may be accomplished by modifying the surface of quantum dots with certain functional groups.In this review, we explore various methods for synthesizing carbon quantum dots, delving into their advanced approach for efficient water treatment. Additionally, we examine the recent developments inCQDs research, with a particular focus ontheir utility in detecting and monitoring heavy metal contaminants.
Microbial oil from R. opacus: Sustainable biodiesel production Zainab Mahmood, Lalit Kumar Singh Research Journal of Chemistry and Environment, 2024 Generating additional and efficient biodiesel routes will rely heavily on advancements in clean energy technologies along with the creation of novel fermentation tactics and analyses. To create biofuels of the second generation from alternative carbon sources other than food, oleaginous microbes with the capacity to accumulate methyl esters of fatty acids (FAMEs), such as Rhodococcus species, can be utilised. These "micro biorefineries" offer a technique to convert waste streams from industry and agriculture into fungible fuels or compounds that can be used as building blocks for other substances and chemicals. Due to their similar content to typical raw materials like plant-based oils, oils from bacteria have become a feasible substitute to create biodiesel from raw material. The fact that they do not compete for arable land or with food for people or animals is another benefit of them. Using hydrolysate as the only source of carbon, a carbon and energy balance for the model species R. opacus was assessed in this study. A favourable energy balance was shown by the method. The viability of bacterial oil as a biofuel substrate was also assessed by measuring the lipid fatty acid profile and cetane number. Also, an analysis of the oil yields from bacterial, microalgae and plant-based oils is shown in terms of how much land each uses. The findings revealed that R. opacus oil production is significantly more than plant-based oils (650 times) and microalgae (30 times).
Crude Microbial Enzymes (Fungal Enzymes) from Agro-Wastes as a Potent Agent for Biological Pretreatment of Algal Biomass Processing to Biofuel Production Sangita Mahapatra, Lalit Kumar Singh Biofuels Scientific Explorations and Technologies for A Sustainable Environment, 2024 Microbial enzymes produced from fungal and bacterial strains have shown a wide range of applications in recent times due to their extensive mechanism applicable in various industries. An exciting approach of fungal enzymes pertains to its application for the pretreatment of algal biomass to produce biofuels as an alternative to conventional petroleum-based fuels. Today, a major concern appearing in biofuel production is the hydrolysis of algal biomass to obtain potential precursors for biofuel production. In this context, biological pretreatment offers an effective method for biomass deconstruction compared to conventional means (physical and chemical). The cell wall of algal biomass is made of cellulose, hemicelluloses, xylans, and other polysaccharides. Therefore, fungal or bacterial species are appropriate in biological pretreatment as they can naturally produce enzymes such as cellulase, xylanase, amylase, pectinase, chitosanase, and achromopeptidase that can degrade the algal cell wall without hampering the internal cell constituents. One of the significant advantages of utilizing fungal enzymes over other strains is its higher efficiency and downstream yields. The present paper aims to discuss various fungal enzymes that can be used for the pretreatment of algal biomass for the production of microalgal biofuel, and their associated advatanges and limitations.
Food waste: an emerging trend to convert waste into energy Prateek Mishra, Sandhya Sompura, Surbhi Sharma, Ju-Hyeong Jung, Swati Punetha, Suneetha Vuppu, Lalit Kumar Singh Waste to Energy Sustainable Approaches for Emerging Economies, 2024
Strengthening bioenergy-based economy through conversion of wastewater resources: An insight to application of extremophiles Extremophiles Wastewater and Algal Biorefinery, 2023
Comparative studies to determine the effect of the media constituents for acid protease production by submerged fermentation Asian Journal of Microbiology Biotechnology and Environmental Sciences, 2008
RECENT SCHOLAR PUBLICATIONS
Application 12 Biohydrogen of Production Biochar in P Mishra, S Sompura, ME Medina, JLSV Villafuerte, LK Singh Biochar and the Circular Economy: Innovations in Production and Applications … , 2026 2026
Application 11 Anaerobic Digestion of Biochar in H Gupta, P Mishra, LK Singh, SP Chaurasia, V Vivekanand Biochar and the Circular Economy: Innovations in Production and Applications … , 2026 2026
Application of Biochar in Biohydrogen Production P Mishra, S Sompura, MEM Villafuerte, JLS Valdivia, LK Singh Biochar and the Circular Economy, 189-200 , 2025 2025
Application of Biochar in Anaerobic Digestion H Gupta, P Mishra, LK Singh, SP Chaurasia, V Vivekanand Biochar and the Circular Economy, 168-188 , 2025 2025
Bioelectrochemical Systems for Sustainable Treatment of Industrial Wastewater: Current Status and Future Prospects S Karmakar, LK Singh, R Rani Biotechnological Applications in Industrial Waste Valorization, 23-42 , 2025 2025
Bioelectrochemical Systems for Sustainable S Karmakar, LK Singh, R Rani Biotechnological Applications in Industrial Waste Valorization, 23 , 2025 2025
Food waste: an emerging trend to convert waste into energy P Mishra, S Sompura, S Sharma, JH Jung, S Punetha, S Vuppu, LK Singh Waste-to-Energy, 219-247 , 2025 2025 Citations: 1
Metabolic engineering of lipid biosynthesis pathway to enhance the oil content in microalgae Z Mahmood, M Nigam, LK Singh Recent advances in bioprocess engineering and bioreactor design, 37-63 , 2024 2024 Citations: 1
Crude Microbial Enzymes (Fungal Enzymes) From Agro-Wastes as a Potent Agent for Biological Pretreatment of Algal Biomass Processing to Biofuel Production S Mahapatra, LK Singh Biofuels, 335-349 , 2024 2024 Citations: 1
Technology development and challenges for the transformation of municipal solid waste into sustainable energy production T Raj, S Sompura, K Chandrasekhar, SK Singh, S Pandey, LK Singh, ... Biomass and Bioenergy 178, 106965 , 2023 2023 Citations: 41
Strengthening Bioenergy-Based Economy Through Conversion of Wastewater Resources B Agarwal, LK Singh Extremophiles: Wastewater and Algal Biorefinery, 1 , 2023 2023
Strengthening Bioenergy-Based Economy Through Conversion of Wastewater Resources: An Insight to Application of Extremophiles B Agarwal, LK Singh Extremophiles, 1-22 , 2023 2023
Rhodococcus opacus high-cell-density batch cultivation with a bagasse hydrolysate for possible triacylglycerol synthesis Z Mahmood, LK Singh Biomedical and Biotechnology Research Journal (BBRJ) 7 (2), 209-217 , 2023 2023 Citations: 9
Machine learning in fermentative biohydrogen production: advantages, challenges, and applications AK Pandey, J Park, J Ko, HH Joo, T Raj, LK Singh, N Singh, SH Kim Bioresource technology 370, 128502 , 2023 2023 Citations: 67
Immobilization of Laccase Enzyme and its Application E Dwivedi, LK Singh J Clin Bio Med Adv 2 (1) 01 4 , 2023 2023
Insight Into Biosorption of Hexavalent Chromium Using Isolated Species Aspergillus Proliferans LA: A Systemic and: In silico: Studies A Shukla, LK Singh Biomedical and Biotechnology Research Journal (BBRJ) 7 (1), 83-92 , 2023 2023 Citations: 1
Increasing The Yield of Bacteriocin Produced by Lactobacillus Planta-rum Using Suspension Culture, J of Vacci Medicine & Health Care 1 (1), 01-04 E Dwivedi, LK Singh Page , 2023 2023
Impact of microplastics and nanoplastics interactions with other contaminants in environment A Pathak, LK Singh Current Developments in Biotechnology and Bioengineering, 333-359 , 2023 2023 Citations: 8
Advances in Biofeedstocks and Biofuels, Production Technologies for Solid and Gaseous Biofuels LK Singh, G Chaudhary John Wiley & Sons , 2022 2022 Citations: 3
Application of Biotechnology for Providing Alternative of Fossil Fuel to Protect Environment LK Singh, G Awasthi, M Bajpai Innovations in Environmental Biotechnology, 805-822 , 2022 2022 Citations: 2
MOST CITED SCHOLAR PUBLICATIONS
Bioconversion of lignocellulosic fraction of water-hyacinth (Eichhornia crassipes) hemicellulose acid hydrolysate to ethanol by Pichia stipitis A Kumar, LK Singh, S Ghosh Bioresource technology 100 (13), 3293-3297 , 2009 2009 Citations: 273
Alkaline pretreatment methods followed by acid hydrolysis of Saccharum spontaneum for bioethanol production G Chaudhary, LK Singh, S Ghosh Bioresource technology 124, 111-118 , 2012 2012 Citations: 121
Phytohormones producing fungal communities: metabolic engineering for abiotic stress tolerance in crops P Tiwari, M Bajpai, LK Singh, S Mishra, AN Yadav Agriculturally important fungi for sustainable agriculture: Volume 1 … , 2020 2020 Citations: 95
Machine learning in fermentative biohydrogen production: advantages, challenges, and applications AK Pandey, J Park, J Ko, HH Joo, T Raj, LK Singh, N Singh, SH Kim Bioresource technology 370, 128502 , 2023 2023 Citations: 67
Development of sequential-co-culture system (Pichia stipitis and Zymomonas mobilis) for bioethanol production from Kans grass biomass LK Singh, CB Majumder, S Ghosh Biochemical engineering journal 82, 150-157 , 2014 2014 Citations: 46
Utilization of hemicellulosic fraction of lignocellulosic biomaterial for bioethanol production LK Singh, G Chaudhary, CB Majumder, S Ghosh Adv Appl Sci Res 2 (5), 508-521 , 2011 2011 Citations: 44
Technology development and challenges for the transformation of municipal solid waste into sustainable energy production T Raj, S Sompura, K Chandrasekhar, SK Singh, S Pandey, LK Singh, ... Biomass and Bioenergy 178, 106965 , 2023 2023 Citations: 41
Portraying fungal mechanisms in stress tolerance: perspective for sustainable agriculture P Tiwari, M Bajpai, LK Singh, AN Yadav, H Bae Recent Trends in Mycological Research: Volume 1: Agricultural and Medical … , 2021 2021 Citations: 39
Anaerobic digestion as a sustainable biorefinery concept for waste to energy conversion A Kushwaha, V Mishra, V Gupta, S Goswami, PK Gupta, LK Singh, ... Waste-to-energy approaches towards zero waste, 129-163 , 2022 2022 Citations: 30
Bioconversion of Hemicellulosic Fraction of Perennial Kans Grass ( Saccharum spontaneum ) Biomass to Ethanol by Pichia stipitis : A Kinetic Study LK Singh, CB Majumder, S Ghosh International journal of green energy 9 (5), 409-420 , 2012 2012 Citations: 17
Advances in biofeedstocks and biofuels, production technologies for biofuels LK Singh, G Chaudhary John Wiley & Sons , 2017 2017 Citations: 15
Microbial production and applications of L-lysine AK Pandey, K Pandey, LK Singh Innovations in Food Technology: Current Perspectives and Future Goals, 211-229 , 2020 2020 Citations: 13
Explore the perennial Kans grass ( Saccharum spontaneum ) biomass for releasing reducing sugars and its optimization. LK Singh, GC Gaurav Chaudhary, CB Majumder, SG Sanjoy Ghosh 2011 Citations: 13
Response surface and artificial neural network simulation for process design to produce L-lysine by Corynebacterium glutamicum NCIM 2168. AK Pandey, K Pandey, A Pandey, VK Morya, LK Singh Indian journal of biotechnology 18 (3) , 2019 2019 Citations: 11
Advances in Biofeedstocks and Biofuels, Biofeedstocks and Their Processing LK Singh, G Chaudhary John Wiley & Sons , 2016 2016 Citations: 11
Phytoremediation: An efficient approach for bioremediation of organic and metallic ions pollutants D Gupta, LK Singh, AD Gupta, V Babu Bioremediation and sustainability: Research and applications, 213-240 , 2012 2012 Citations: 11
Rhodococcus opacus high-cell-density batch cultivation with a bagasse hydrolysate for possible triacylglycerol synthesis Z Mahmood, LK Singh Biomedical and Biotechnology Research Journal (BBRJ) 7 (2), 209-217 , 2023 2023 Citations: 9
Impact of microplastics and nanoplastics interactions with other contaminants in environment A Pathak, LK Singh Current Developments in Biotechnology and Bioengineering, 333-359 , 2023 2023 Citations: 8
A new efficient method for removal of metal cutting fluids from machining waste water A Katiyar, AK Singh, LK Singh International Journal of Engineering and Technical Research.(Special Issue … , 2014 2014 Citations: 8
Studies on recovery of heavy metals from tannery wastewater A Shukla, Z Mahmood, LK Singh International Journal of Engineering, Science and Technology 13 (1), 76-80 , 2021 2021 Citations: 7