Lalit Kumar Singh

@hbtu.ac.in

Professor, Department of Biochemical Engineering
Harcourt Butler Technical University Kanpur



              

https://researchid.co/proflalit

EDUCATION

B. Tech.
M. Tech.
Ph.D.

RESEARCH, TEACHING, or OTHER INTERESTS

Renewable Energy, Sustainability and the Environment, Biotechnology, Process Chemistry and Technology, Waste Management and Disposal

32

Scopus Publications

Scopus Publications

  • Microbial oil from R. opacus: Sustainable biodiesel production
    Zainab Mahmood and Lalit Kumar Singh
    World Researchers Associations
    Generating additional and efficient biodiesel routes will rely heavily on advancements in clean energy technologies along with the creation of novel fermentation tactics and analyses. To create biofuels of the second generation from alternative carbon sources other than food, oleaginous microbes with the capacity to accumulate methyl esters of fatty acids (FAMEs), such as Rhodococcus species, can be utilised. These "micro biorefineries" offer a technique to convert waste streams from industry and agriculture into fungible fuels or compounds that can be used as building blocks for other substances and chemicals. Due to their similar content to typical raw materials like plant-based oils, oils from bacteria have become a feasible substitute to create biodiesel from raw material. The fact that they do not compete for arable land or with food for people or animals is another benefit of them. Using hydrolysate as the only source of carbon, a carbon and energy balance for the model species R. opacus was assessed in this study. A favourable energy balance was shown by the method. The viability of bacterial oil as a biofuel substrate was also assessed by measuring the lipid fatty acid profile and cetane number. Also, an analysis of the oil yields from bacterial, microalgae and plant-based oils is shown in terms of how much land each uses. The findings revealed that R. opacus oil production is significantly more than plant-based oils (650 times) and microalgae (30 times).

  • Food waste: an emerging trend to convert waste into energy
    Prateek Mishra, Sandhya Sompura, Surbhi Sharma, Ju-Hyeong Jung, Swati Punetha, Suneetha Vuppu, and Lalit Kumar Singh
    Elsevier

  • Myeloperoxidase enzyme-catalyzed breakdown of zero-dimension carbon quantum dots
    Pooja Singh and Lalit Kumar Singh
    Frontiers Media SA
    Carbon quantum dots (CQDs) have shown considerable interest in multiple fields including bioimaging, biosensing, photocatalysis, ion sensing, heavy metal detection, and therapy due to highly tunable photoluminescence and good photostability. Apart from having optical properties CQDs offer several advantages such as low toxicity, environmental friendliness, affordability, and simple synthesis methods. Furthermore, by modifying their surface and functionality, it's possible to precisely control their physical and chemical characteristics. Nevertheless, the growing utilization of carbon-based nanomaterials (CNMs) requires thorough examination of their potential toxicity and long-term impacts on human health and biological systems. In this study, carbon quantum dots (CQDs) were synthesized via a microwave-assisted method using citric acid and urea as precursors, resulting in an average particle diameter of 10.73 nm. The CQDs were further characterized using SEM and FTIR analysis. The CQDs exhibited an excitation wavelength of 320 nm, displaying an emission peak at 430 nm. The enzymatic biodegradation of CQDs by human myeloperoxidase enzyme has been thoroughly investigated here. It is very crucial to understand how these carbon quantum dots interact with the innate immune system that plays a vital role in recognizing and clearing foreign particles. Human myeloperoxidase (MPO), a key enzyme highly expressed in neutrophil granulocytes during inflammatory responses, has been shown to facilitate the biodegradation of carbon quantum dots and various carbon-based nanomaterials through oxidative processes. As a member of the peroxidase family, MPO produces hypochlorous acid (HOCl) and a range of reactive intermediates to eliminate pathogens. Consequently, the study of the biodegradability of CQDs within biological systems is essential for accelerating technological advancements. Here, we have assessed breakdown of CQDs through an oxidative process facilitated by a myeloperoxidase (MPO)-based peroxide system. The human MPO enzyme acted as a catalyst for the CQD degradation, and the addition of hydrogen peroxide (H2O2) and sodium chloride (NaCl) was found to accelerate the reaction.

  • Metabolic Engineering of Lipid Biosynthesis Pathway to Enhance the Oil Content in Microalgae
    Zainab Mahmood, Mohit Nigam, and Lalit Kumar Singh
    Springer Nature Singapore


  • Enhanced Production of Microbial Lipid from R. opacus using Bagasse Hydrolysate
    Zainab Mahmood and Lalit Kumar Singh
    World Researchers Associations
    One alternative to traditional fossil fuels is biodiesel. It has a negative impact on nutritional supply because it is mostly manufactured from vegetable oils. Due to the possibility that oleaginous microbe lipids (such as those produced by bacteria, fungi and yeasts) could serve as an alternative raw material for the synthesis of biodiesel, the biomass and lipid buildup of Rhodococcus opacus were examined in several environmental settings. The study's current objective is to use the Response surface methodology's central composite design (CCD) to discover the ideal values of specific variables. Rhodococcus opacus, an oleaginous bacterium, was subjected to a CCD design using independent variables such as inoculum ratio, nitrogen concentration and magnesium concentration. The response variable used for the analysis was lipid content. Regarding the experimental data's lipid content%, a polynomial with a second-order model yielded good results (R2 = 96, (P ≤ 0.01)). Rhodococcus opacus produced the best results in the trials with glucose and hydrolysate with biomass concentrations of 4.75 g/L and 4.55 g/L and lipid contents of 71.18% and 66.55% respectively at pH 7. Compared to the starting values prior to optimization, these demonstrate a boost in the lipid concentration and an enhancement in the lipid coefficient. This study further supports the viability of R. opacus as a variant for the generation of biofuel from readily available, low-cost biomass resources.

  • Technology development and challenges for the transformation of municipal solid waste into sustainable energy production
    Tirath Raj, Sandhya Sompura, K. Chandrasekhar, Sushil Kumar Singh, Srinath Pandey, Lalit Kumar Singh, Manish Singh Rajput, Deepak Kumar, Shashi Kant Bhatia, Anil Kumar Patel,et al.
    Elsevier BV

  • Strengthening bioenergy-based economy through conversion of wastewater resources: An insight to application of extremophiles


  • Rhodococcus opacus high-cell-density batch cultivation with a bagasse hydrolysate for possible triacylglycerol synthesis
    LalitKumar Singh and Zainab Mahmood
    Medknow
    Background: The promising use of lignocellulosic materials as a substrate for the synthesis of biochemical and biofuels has long been recognized. The bioconversion of all lignocellulosic sugars into useful products results in high sugar yields, which makes lignocelluloses appealing for use. Pretreatment and hydrolysis of lignocelluloses at high solids loadings are vital throughout industrial processes, making them more cost-effective and ecologically friendly by lowering the need for capital expenditures, energy use, and water use. The high expense of fermentation medium hinders heterotrophic oleaginous microorganisms' ability to metabolically synthesize single-cell oils for biodiesel use. This study's objective was to ascertain whether bagasse hydrolysate could be used as a cost-effective feedstock for oleaginous Rhodococcus opacus to produce microbial lipids. Methods: R. opacus a strain was used in this study. The culture was first of all maintained at soytone nutrient growth medium (i.e. GROTH MEDIUM 56) and subcultured at regular intervals and as well as preserved at 4°C. For the production media, a minimal salt medium is used. Sugarcane bagasse is pretreated by acidic hydrolysis followed by detoxification of hydrolysate and lipid extraction is done by Folch's extraction process. Results: After a brief adaptation/lag phase, the strain showed a viable growth pattern once the nutrients were detoxicated through the removal of inhibitors (e.g. hydroxymethyl-furfural [HMF]). R. opacus was grown via shake flask fermentation in bagasse hydrolysate with different sugar concentrations. When cultivated in S2 experiment, R. opacus obtained over 50% of its dry cell weight as a lipid with a lipid yield of 64.47% and productivity is 0.015 g/L/h. Conclusions: Depending on the available substrates, different microbial oils had different compositions. These findings suggest that lignocellulosic hydrolysate can be utilized as an inexpensive fermentation substrate for the R. opacus to produce microbial lipids. As a result, the range of uses for aqueous liquors produced by lignocellulosic pretreatment has increased, enabling further improvement.

  • Machine learning in fermentative biohydrogen production: Advantages, challenges, and applications
    Ashutosh Kumar Pandey, Jungsu Park, Jeun Ko, Hwan-Hong Joo, Tirath Raj, Lalit Kumar Singh, Noopur Singh, and Sang-Hyoun Kim
    Elsevier BV

  • A comparative study on the removal and recovery of hexavalent chromium from tannery wastewater using an isolated strain Aspergillus proliferans LA and a known strain Aspergillus terreus
    Abhimati Shukla and Lalit Kumar Singh
    World Researchers Associations
    Isolation of a potent microorganism from the soil near tanneries and a comparative analysis of the removal and recovery of different concentrations of hexavalent chromium using known strain Aspergillus terreus and isolated strain Aspergillis proliferansLA are done. The isolated fungal species from the chromium contaminated soil sites located in the industrial area of Kanpur, U.P. were tested for its potential for the removal of hexavalent chromium from wastewater streams. The experiments were conducted comparing the biosorption efficiency of the isolated species Aspergillus proliferansLA and Aspergillus terreus by varying the initial Cr (VI) concentration and media constituents. The highest removal efficiency of 99.19% was shown by the isolated species Aspergillus proliferansLA for an initial Cr (VI) concentration of 10ppm followed by increasing the concentration to 50ppm whereas Aspergillus terreus showed 78.43% removal of hexavalent chromium. This was then followed by desorption using 0.8M NaOH and 75.12% hexavalent chromium was desorbed from the cells. The isolated strain Aspergillus proliferansLA was seen to show better removal and recovery results than the known strain Aspergillus terreus.

  • Insight into biosorption of hexavalent chromium using isolated species Aspergillus Proliferans LA: A systemic and In silico studies
    LalitKumar Singh and Abhimati Shukla
    Medknow
    Background: The wastewater disposal into the water bodies without removing the toxic heavy metals and other industrial impurities is a major issue these days. These heavy metals cause serious health issues to the human and animal life and also harm the environment and reduce the productivity of crops. A potent microorganism resistant to hexavalent chromium was isolated. The activity of this isolated strain was analyzed using in silico studies. Methods: In this study, a chromium-resistant fungus was isolated from the soil of the dumping sites of the tanneries in Kanpur, UP, India, followed by isolation by serial dilution, plating method, and finally, genome sequencing. It was identified as Aspergillus proliferans LA that is submitted to the National Collection for Industrial Microorganisms (NCIM) database with accession no. NCIM-1473. In the current study, the comparative analysis of the protein sequence of A. proliferans (NCIM-1473) was done against the known 53 protein sequences of the fungus and bacterial strains already reported for their chromium-resistant nature. The physical and chemical parameters of the known and isolated chromium-resistant proteins were analyzed using the ProtParam tool. The comparative study on the sequence and structural alignment of known and isolated chromium-resistant protein was done using EMBOSS-NEEDLE and FATCAT, respectively. Results: In this analysis, the top 10 strains showing similarity with A. proliferans (NCIM-1473) were reported and among which ChrI, chromium regulatory protein Cupriavidus metallidurans CH34 was showing maximum similarity with isolated chromium resistant protein for all the analysis, namely ProtParam, sequence, and FATCAT analysis. This strain, Cupriavidus metallidurans CH34, has been reported resistant against eight heavy metals, one of which is chromate, and was first identified in the heavy-metal contaminated sludge in a settling tank of Belgium. Conclusion: These studies conclude that the strain isolated in our laboratory (accession no. NCIM-1473) is potentially chromium resistant and a unique strain.


  • Application of Biotechnology for Providing Alternative of Fossil Fuel to Protect Environment
    Lalit Kumar Singh, Garima Awasthi, and Mangalam Bajpai
    Springer Nature Singapore



  • Anaerobic digestion as a sustainable biorefinery concept for waste to energy conversion
    Anamika Kushwaha, Vivek Mishra, Veena Gupta, Shivani Goswami, Pankaj Kumar Gupta, Lalit Kumar Singh, Chandra Bhanu Gupt, Kaustubh Rakshit, and Lalit Goswami
    Elsevier

  • Omics approaches for infectious diseases
    Amrendra Nath Pathak, Lalit Kumar Singh, and Esha Dwivedi
    Springer Singapore

  • Production and applications of polylactic acid
    Ashutosh Kumar Pandey, Ranjna Sirohi, Sudha Upadhyay, Mitali Mishra, Virendra Kumar, Lalit Kumar Singh, and Ashok Pandey
    Elsevier

  • Kinetic study of dyes degradation by Aspergillus niger in submerged fermentation


  • Microbial Production and Applications of L-lysine
    Ashutosh Kumar Pandey, Kritika Pandey, and Lalit Kumar Singh
    Springer Singapore

  • Sugar and sugar alcohols: Xylitol



  • Advances in Biofeedstocks and Biofuels
    L. Singh and G. Chaudhary
    Wiley


RECENT SCHOLAR PUBLICATIONS