Dr. Arvind Kumar Shukla is working on the molecular mechanism of cancer stem cell biology. He is the main focus in the fabrication of functional tissue and organ development by 3D bioprinting, with a study on stem cells & cancer signaling pathways, and also focuses on various cancer drug delivery methods that are used and tested clinically for treatment.
EDUCATION
Doctor of Philosophy ( in Biomedical Convergence Engineering
Specialization: 3D Bioprinting, Organ-on-a-Chip (OOC), Stem Cells, Cancer
Pusan National University, Busan, South Korea
February 2020 – August 2025
Master of Business Administration (MBA) in Health Care Management
Dr. D.Y. Patil Vidyapeeth, Pune, India
July 2022 – August 2024
Master of Science (M.Sc.) in Genetic Engineering
Focus: HIV, Stem Cells, Cancer
Dr. D.Y. Patil University, Mumbai, India
July 2012 – July 2014
Bachelor of Science (B.Sc.) in Zoology and Environmental Science
University of Mumbai, Mumbai, India
February 2008 – July 2012
RESEARCH, TEACHING, or OTHER INTERESTS
Cell Biology, Molecular Biology, Genetics, General Pharmacology, Toxicology and Pharmaceutics
Integrating 3D Bioprinting with Organoid Technology-Based Breast Cancer Models for Drug Evaluation Arvind Kumar Shukla, Sandhya Shukla, Raj Kumar Mongre, Adarsha Mahendra Upadhyay, Govindhan Thiruppathi, et al. Organoids, 2025 Breast cancer remains one of the leading causes of cancer morbidity and mortality among women worldwide. Conventional two-dimensional (2D) cell culture models and animal studies often fail to accurately recapitulate the complex tumor microenvironment and heterogeneous nature of breast cancer. Recent advancements in tissue engineering have enabled the development of more physiologically relevant models using three-dimensional (3D) bioprinting and organoid technology. This study focuses on integrating 3D bioprinting with patient-derived organoid models to replicate breast cancer tissue architecture, cellular heterogeneity, and tumor-stroma interactions. Utilizing biomimetic bioinks and customized bioprinting protocols, we successfully fabricated breast cancer tissue constructs embedded with stromal and immune components. These engineered models demonstrated high fidelity in mimicking in vivo tumor pathophysiology, including angiogenesis, epithelial–mesenchymal transition, and extracellular matrix remodeling. Furthermore, the platform allowed for high-throughput drug screening and evaluation of therapeutic responses, revealing differential sensitivities to chemotherapeutics and targeted therapies. Our findings highlight the potential of bioprinted organoid models as powerful tools for personalized medicine, enabling more predictive and reliable cancer research and drug development.
High-Throughput 3D Bioprinted Organoids of Skin Cancer Utilized for Diagnosis and Personalized Therapy Arvind Kumar Shukla, Sandhya Shukla, Sonali Pradeep Suryawanshi, Adarsha Mahendra Upadhyay, Navin Ray, et al. Current Oncology, 2025 Recent advancements in three-dimensional (3D) bioprinting have revolutionized the modeling of skin cancer, enabling the fabrication of high-throughput, patient-specific organoids that recapitulate the structural, functional, and microenvironmental complexity of native tumors. This review focuses on the integration of cutting-edge bioprinting technologies with bioengineered extracellular matrices and patient-derived cells to generate physiologically relevant skin cancer models for diagnostic and personalized medicine applications. Key technological innovations, including novel bioinks, multi-material printing strategies, and biomimetic approaches, are discussed for their ability to replicate tumor-stroma interactions, vascularization, and immune microenvironments. The utility of bioprinted organoids in high-throughput drug screening, mutation-targeted therapy design, and biomarker discovery is critically evaluated. Additionally, we address current challenges in standardization, reproducibility, and clinical translation, highlighting regulatory and quality-control considerations. Collectively, this review emphasizes the transformative potential of 3D bioprinted skin cancer organoids as platforms for precision oncology, bridging bioengineering advances with translational research to accelerate therapeutic development and personalized treatment strategies.
3D Bioprinting-Assisted Engineering of Stem Cell-Laden Hybrid Biopatches With Distinct Geometric Patterns Considering the Mechanical Characteristics of Regular and Irregular Connective Tissues Minjun Ahn, Gyu Tae Park, Arvind Kumar Shukla, Boguen Kwon, Jae Ho Kim, et al. Advanced Healthcare Materials, 2025 Connective tissues display distinct mechanical behaviors, ranging from unidirectional stiffness in regular tissues to multidirectional compliance in irregular tissues. Replicating these biomechanical characteristics in engineered constructs remains a key challenge in regenerative medicine. This study presents a novel biofabrication platform for hybrid biopatches composed of tonsil‐derived mesenchymal stem cell (TMSC)‐laden collagen bioink reinforced with 3D printed polymeric patterns. Two distinct geometries, chiral and chevron, are designed to emulate the mechanical behavior of irregular and regular connective tissues, respectively. Mechanical testing shows that the chiral pattern exhibits quasi‐isotropic behavior with balanced stiffness and extensibility, whereas the chevron pattern demonstrate anisotropic mechanical properties. These mechanical features are maintained within the hybrid biopatches, leading to enhanced tensile strength and fatigue resistance compared with constructs composed solely of TMSC‐laden collagen. In a porcine mucosal defect model, the chiral‐patterned hybrid biopatch promoted superior epithelial repair, evidenced by narrower wound margins, continuous epithelial layers, and elevated expression of epithelial markers. These results suggest that mechanical compatibility with host tissue influences regenerative outcomes. Collectively, this study highlights the potential of incorporating geometric polymer patterns as a strategy for engineering tissue‐specific mechanics and improving regenerative performance, offering a promising platform for soft connective tissue repair.
Targeting Pathways and Mechanisms in Gynecological Cancer with Antioxidant and Anti-Inflammatory Phytochemical Drugs Sandhya Shukla, Arvind Shukla, Navin Ray, Adarsha Upadhyay, Fowzul Fahad, et al. Onco, 2025 Globally, women’s cancer-related morbidity and death are still caused mainly by gynecologic cancer. Antioxidant and anti-inflammatory drugs have shown promise in treating gynecologic cancer because of the complex interactions among oxidative stress, inflammation, and the development of tumors. This review focuses on how these drugs, which include polyphenols, terpenoids, and thiols-related phytochemical-derived compounds target different pathways associated with developing and progressing gynecologic cancer. We investigate what factors affect the tumor microenvironment, specifically how they affect immunological response and vasculogenesis. Through the review of recent studies, we have gained an extensive understanding of the molecular pathways that anti-inflammatory and antioxidant drugs use to achieve their therapeutic benefits. Gynecologic cancer is still a potent cause of cancer-related deaths and fatalities for women globally. Antioxidant and anti-inflammatory drugs have shown promise in treating gynecologic cancer because of the complex interactions among oxidative stress, inflammation, and the development of tumors. This review focuses on how these drugs target different pathways associated with developing and progressing gynecologic cancer. We investigate what factors affect the tumor microenvironment, specifically how they affect immunological response and vasculogenesis. Through the review of recent studies, we have gained an extensive understanding of the molecular pathways that anti-inflammatory and antioxidant drugs use to achieve their therapeutic benefits.
Molecular Insight and Antioxidative Therapeutic Potentials of Plant-Derived Compounds in Breast Cancer Treatment Sandhya Shukla, Arvind Shukla, Adarsha Upadhyay, Navin Ray, Fowzul Fahad, et al. Onco, 2025 Breast cancer is one of the most common and difficult-to-treat cancers affecting women globally. Long-term treatment success is still limited by problems like drug resistance, toxicity, and recurrence, even with advancements in conventional therapies. The application of substances derived from plants for medical purposes, or phytotherapy, has become a viable adjunctive approach to the treatment of breast cancer. An integrative approach to phytotherapy is examined in this review, focusing on how it can alter important molecular pathways implicated in the development, progression, and metastasis of breast cancer. By focusing on important signaling cascades like TGF-β, Wnt, Hedgehog, Notch, IL-6, Integrins, VEGF, HER2, EGFR, PI3K/Akt, and MAPK, and estrogen receptor pathways, a variety of phytochemicals, such as flavonoids, alkaloids, terpenoids, and polyphenols, demonstrate strong anticancer effects. This review also discusses how they affect immune modulation, angiogenesis, cell cycle regulation, and apoptosis. Moreover, it also emphasizes the challenges with these natural compounds’ bioavailability, standardization, and clinical translation while highlighting preclinical and clinical research that supports their therapeutic potential. This review attempts to give a thorough grasp of how plant-based compounds can support efficient and focused breast cancer treatments by fusing molecular insights with phytotherapeutic approaches.
Advancement in Cancer Vasculogenesis Modeling through 3D Bioprinting Technology Arvind Kumar Shukla, Sik Yoon, Sae-Ock Oh, Dongjun Lee, Minjun Ahn, et al. Biomimetics, 2024 Cancer vasculogenesis is a pivotal focus of cancer research and treatment given its critical role in tumor development, metastasis, and the formation of vasculogenic microenvironments. Traditional approaches to investigating cancer vasculogenesis face significant challenges in accurately modeling intricate microenvironments. Recent advancements in three-dimensional (3D) bioprinting technology present promising solutions to these challenges. This review provides an overview of cancer vasculogenesis and underscores the importance of precise modeling. It juxtaposes traditional techniques with 3D bioprinting technologies, elucidating the advantages of the latter in developing cancer vasculogenesis models. Furthermore, it explores applications in pathological investigations, preclinical medication screening for personalized treatment and cancer diagnostics, and envisages future prospects for 3D bioprinted cancer vasculogenesis models. Despite notable advancements, current 3D bioprinting techniques for cancer vasculogenesis modeling have several limitations. Nonetheless, by overcoming these challenges and with technological advances, 3D bioprinting exhibits immense potential for revolutionizing the understanding of cancer vasculogenesis and augmenting treatment modalities.
Vascularization strategies for human skin tissue engineering via 3D bioprinting Arvind Kumar Shukla, Dongjun Lee, Sik Yoon, Minjun Ahn, Byoung Soo Kim International Journal of Bioprinting, 2024 The skin is composed of many cells that are organized into different layers and connected by dense and complex vascular networks. This creates a dynamic microenvironment in which cells interact within the matrix. Significant advancements have been made in this field over the past decade, and various strategies have been developed for accelerating and enhancing skin regeneration. The primary challenge for successful skin grafts is the integration of the functional vasculature, which can supply essential nutrients and oxygen to cell-laden structures and damaged native tissues. An inadequate vascular network can lead to ischemia, which can cause slow wound healing—particularly in the case of chronic skin conditions. Therefore, blood vessel formation remains one of the most significant obstacles that skin tissue engineering must overcome to create vascularized skin tissue substitutes with specific living cells. Technological advances can augment effective vascularization. The three-dimensional (3D) bioprinting platform is a promising technology that allows precise deposition of living cells and bioactive materials. The application of this technology to skin tissue engineering can provide solutions for augmenting pre-vascularization in engineered in vitro skin models and in vivo skin substitutes. This review presents the significance of skin vascularization in in vitro modeling and in vivo wound healing. Various strategies and related applications involving 3D bioprinting technology are introduced for the biofabrication of enhanced vascularized skin in vitro and in vivo, followed by a discussion of their limitations and future research directions.
Applications of 3D Bioprinting Technology in Induced Pluripotent Stem Cells-Based Tissue Engineering Arvind Kumar Shukla, Ge Gao, Byoung Soo Kim Micromachines, 2022 Induced pluripotent stem cells (iPSCs) are essentially produced by the genetic reprogramming of adult cells. Moreover, iPSC technology prevents the genetic manipulation of embryos. Hence, with the ensured element of safety, they rarely cause ethical concerns when utilized in tissue engineering. Several cumulative outcomes have demonstrated the functional superiority and potency of iPSCs in advanced regenerative medicine. Recently, an emerging trend in 3D bioprinting technology has been a more comprehensive approach to iPSC-based tissue engineering. The principal aim of this review is to provide an understanding of the applications of 3D bioprinting in iPSC-based tissue engineering. This review discusses the generation of iPSCs based on their distinct purpose, divided into two categories: (1) undifferentiated iPSCs applied with 3D bioprinting; (2) differentiated iPSCs applied with 3D bioprinting. Their significant potential is analyzed. Lastly, various applications for engineering tissues and organs have been introduced and discussed in detail.
Hazardous waste management in India: Risks and challenges associated with hazardous waste Risks and Challenges of Hazardous Waste Management Reviews and Case Studies, 2020
Integrated CRISPR-Cas9 and 3D Bioprinting Approach for Development and Evaluation of High-Throughput Advanced Organotypic Cancer Models AK Shukla, S Shukla, AM Upadhyay, A Nagappan, RKR Kumar Preprints , 2026 2026 Citations: 1
Advances in Diagnosis and Management of Diabetes in Children and Adults with Focus on Drugs, Insulin, and Dietary Interventions S Shukla, L Palanisamy, D Yadav, AM Upadhyay, AK Shukla, ... Preprints , 2026 2026
High-Throughput 3D Bioprinted Organoids of Skin Cancer Utilized for Diagnosis and Personalized Therapy AK Shukla, S Shukla, SP Suryawanshi, A Mahendra Upadhyay, N Ray, ... Current Oncology 32 (12), 653 , 2025 2025 Citations: 2
Integrating 3D Bioprinting with Organoid Technology-Based Breast Cancer Models for Drug Evaluation AK Shukla, S Shukla, RK Mongre, AM Upadhyay, G Thiruppathi, ... Organoids 4 (4), 26 , 2025 2025 Citations: 1
3D Bioprinting‐Assisted Engineering of Stem Cell‐Laden Hybrid Biopatches With Distinct Geometric Patterns Considering the Mechanical Characteristics of Regular and Irregular … M Ahn, GT Park, AK Shukla, B Kwon, JH Kim, ES Sung, BS Kim Advanced Healthcare Materials 14 (25), 2502763 , 2025 2025 Citations: 8
A Study on Angiogenic Potentials of Endothelial Cell-Patterned Skin Patches Fabricated by In-Bath 3D Bioprinting with Light-Activated Bioink for Improved Wound Healing AK Shukla 2025 Citations: 1
Exploring the angiogenic potential of skin patches with endothelial cell patterns fabricated via in-bath 3D bioprinting using light-activated bioink for enhanced wound healing AK Shukla, M Ahn, J Gao, D Lee, S Yoon, SO Oh, G Gao, WW Cho, ... Biomaterials, 123575 , 2025 2025 Citations: 9
Molecular Insight and Antioxidative Therapeutic Potentials of Plant-Derived Compounds in Breast Cancer Treatment. Onco 2025, 5, 27 S Shukla, AK Shukla, AM Upadhyay, N Ray, FI Fahad, A Nagappan, ... onco 5 (2), 27 , 2025 2025 Citations: 10
Targeting pathways and mechanisms in gynecological cancer with antioxidant and anti-inflammatory phytochemical drugs S Shukla, AK Shukla, N Ray, AM Upadhyay, FI Fahad, SD Dutta, ... Onco 5 (2), 24 , 2025 2025 Citations: 8
Surgical Management of a Rare Case of Mesocolonic Internal Hernia through Congenital Defect in Ascending Mesocolon with Mesenteric Root Torsion AMU ,Sandhya Shukla, Arvind Kumar Shukla, Yan Zhiqiang Asian Journal of Research in Surgery 8 (1), 35-40 , 2025 2025
Emerging Molecular and Clinical Challenges in Managing Lung Cancer Treatment during the Covid-19 Infection S Shukla, N Ray, AK Shukla, AM Upadhyay, G Mirone, RK Mongre Journal of Cancer and Tumor International 14 (4), 143-161 , 2024 2024 Citations: 2
Long-lasting Response of Human Circulating T-follicular Helper Cells (cTfh) To Post SARS-CoV-2 mRNA Immunization S Shukla, AK Shukla, N Ray, AM Upadhyay, G Mirone, RK Mongre Asian Journal of Immunology 7 (1), 228-246 , 2024 2024 Citations: 2
Comparative Analysis of Knowledge, Attitude, and Practices (KAP) for Innovation Management Processes to Enhance Healthcare Delivery in Hospital Administration AK Shukla, S Shukla INTERNATIONAL JOURNAL FOR INNOVATIVE RESEARCH IN MULTIDISCIPLINARY FIELD 10 … , 2024 2024
Advancement in cancer vasculogenesis modeling through 3D Bioprinting Technology AK Shukla, S Yoon, SO Oh, D Lee, M Ahn, BS Kim Biomimetics 9 (5), 306 , 2024 2024 Citations: 15
A Study on the Effect of Bioprinted Skin Patches with Various Vascular Patterns on Wound Healing M Ahn, AK Shukla, WW Cho, DW Cho, BS Kim 한국정밀공학회 학술발표대회 논문집, 140-140 , 2024 2024 Citations: 1
Possible Interaction Between Drugs for COVID19 And Cancer Therapy G Marfe, AK Shukla, G Tarro, C Di Stefano Clinical Medicine And Health Research Journal 4 (2), 839-857 , 2024 2024 Citations: 1
Vascularization strategies for human skin tissue engineering via 3D bioprinting Arvind Kumar Shukla, Dongjun Lee, Sik Yoon, Minjun Ahn, Byoung Soo Kim International Journal of Bioprinting 10 (1), 0(0), 1727. , 2024 2024 Citations: 14
Viral Shedding and Long COVID-19 Disease in Cancer Patients G Marfe, S Perna, G Mirone, AK Shukla Oncology and COVID 19, 83-99 , 2023 2023 Citations: 1
In-bath Bioprinting of Pre-vascularized Skin Patches with Different Geometrical Patterns for Effective Skin Regeneration AK Shukla, MJ Ahn, JS Lee, BS Kim 한국정밀공학회 학술발표대회 논문집, 677-677 , 2023 2023 Citations: 2
Acknowledgment to the Reviewers of Advances in Respiratory Medicine in 2022 A Vervenioti, J Chrzanowski, A Kumar, J Marczak, A Iglesias, ... 2023
MOST CITED SCHOLAR PUBLICATIONS
Hyperactive innate immunity causes degeneration of dopamine neurons upon altering activity of Cdk5 AK Shukla, J Spurrier, I Kuzina, E Giniger Cell reports 26 (1), 131-144. e4 , 2019 2019 Citations: 71
Applications of 3D bioprinting technology in induced pluripotent stem cells-based tissue engineering AK Shukla, G Gao, BS Kim Micromachines 13 (2), 155 , 2022 2022 Citations: 47
Effectiveness of COVID-19 vaccines and their challenges G Marfe, S Perna, AK Shukla Experimental and Therapeutic Medicine 22 (6), 1407 , 2021 2021 Citations: 41
Emerging Importance of Tyrosine Kinase Inhibitors against Cancer: Quo Vadis to Cure? MSL Raj Kumar Mongre, Chandra Bhushan Mishra, Arvind Kumar Shukla, Amresh ... International Journal of Molecular Sciences 22 (21), 11659 , 2021 2021 Citations: 41
Recent advancements in nanoparticle-based optical biosensors for circulating cancer biomarkers C Amri, AK Shukla, JH Lee Materials 14 (6), 1339 , 2021 2021 Citations: 38
Sphingosine kinases signalling in carcinogenesis G Marfe, G Mirone, A Shukla, C Di Stefano Mini reviews in medicinal chemistry 15 (4), 300-314 , 2015 2015 Citations: 36
Acellular dermis as a dermal matrix of tissue engineered skin substitute for burns treatment AK Shukla, N Dey, P Nandi, M Ranjan Ann Public Health Res 2 (3), 1023 , 2015 2015 Citations: 32
Signaling mechanisms of resistance to EGFR-and anti-angiogenic inhibitors cancer G Mirone, A Shukla, G Marfe Critical Reviews in Oncology/Hematology 97, 85-95 , 2016 2016 Citations: 30
Advancement in cancer vasculogenesis modeling through 3D Bioprinting Technology AK Shukla, S Yoon, SO Oh, D Lee, M Ahn, BS Kim Biomimetics 9 (5), 306 , 2024 2024 Citations: 15
Vascularization strategies for human skin tissue engineering via 3D bioprinting Arvind Kumar Shukla, Dongjun Lee, Sik Yoon, Minjun Ahn, Byoung Soo Kim International Journal of Bioprinting 10 (1), 0(0), 1727. , 2024 2024 Citations: 14
Molecular Insight and Antioxidative Therapeutic Potentials of Plant-Derived Compounds in Breast Cancer Treatment. Onco 2025, 5, 27 S Shukla, AK Shukla, AM Upadhyay, N Ray, FI Fahad, A Nagappan, ... onco 5 (2), 27 , 2025 2025 Citations: 10
Exploring the angiogenic potential of skin patches with endothelial cell patterns fabricated via in-bath 3D bioprinting using light-activated bioink for enhanced wound healing AK Shukla, M Ahn, J Gao, D Lee, S Yoon, SO Oh, G Gao, WW Cho, ... Biomaterials, 123575 , 2025 2025 Citations: 9
3D Bioprinting‐Assisted Engineering of Stem Cell‐Laden Hybrid Biopatches With Distinct Geometric Patterns Considering the Mechanical Characteristics of Regular and Irregular … M Ahn, GT Park, AK Shukla, B Kwon, JH Kim, ES Sung, BS Kim Advanced Healthcare Materials 14 (25), 2502763 , 2025 2025 Citations: 8
Targeting pathways and mechanisms in gynecological cancer with antioxidant and anti-inflammatory phytochemical drugs S Shukla, AK Shukla, N Ray, AM Upadhyay, FI Fahad, SD Dutta, ... Onco 5 (2), 24 , 2025 2025 Citations: 8
Optimization of PCR DNA sequencing method for SNP detection in abacavir sensitivity gene A Shukla, A Raut, S Choudhary DNA 260, A280 , 2015 2015 Citations: 4
High-Throughput 3D Bioprinted Organoids of Skin Cancer Utilized for Diagnosis and Personalized Therapy AK Shukla, S Shukla, SP Suryawanshi, A Mahendra Upadhyay, N Ray, ... Current Oncology 32 (12), 653 , 2025 2025 Citations: 2
Emerging Molecular and Clinical Challenges in Managing Lung Cancer Treatment during the Covid-19 Infection S Shukla, N Ray, AK Shukla, AM Upadhyay, G Mirone, RK Mongre Journal of Cancer and Tumor International 14 (4), 143-161 , 2024 2024 Citations: 2
Long-lasting Response of Human Circulating T-follicular Helper Cells (cTfh) To Post SARS-CoV-2 mRNA Immunization S Shukla, AK Shukla, N Ray, AM Upadhyay, G Mirone, RK Mongre Asian Journal of Immunology 7 (1), 228-246 , 2024 2024 Citations: 2
In-bath Bioprinting of Pre-vascularized Skin Patches with Different Geometrical Patterns for Effective Skin Regeneration AK Shukla, MJ Ahn, JS Lee, BS Kim 한국정밀공학회 학술발표대회 논문집, 677-677 , 2023 2023 Citations: 2
Molecular and therapeutic clues in chronic myeloid leukemia G Marfe, G Mirone, A Shukla, C Di Stefano Frontiers in Clinical Drug Research-Hematology: Volume 2, 3-80 , 2016 2016 Citations: 2