Carmen Garcia Montoya
@iib.uam.es
Group of Neuropathology of Hearing and Myelinopathies in the Rare Diseases Department
Sols-Morreale Biomedical Research Institute
EDUCATION
Master in Biochemistry, Master in Biochemistry, Molecular Biology and Biomedicine
Currently PhD student.
RESEARCH, TEACHING, or OTHER INTERESTS
General Biochemistry, Genetics and Molecular Biology, Analytical Chemistry, General Immunology and Microbiology, General Neuroscience
7
Scopus Publications
12
Scholar Citations
3
Scholar h-index
Scopus Publications
- Uncovering cellular senescence as a therapeutic target in NF2-related vestibular schwannoma
Sandra Franco-Caspueñas, Carmen García-Montoya, Julio Contreras, Luis Lassaletta, Isabel Varela-Nieto, and Ana M. Jiménez-Lara
Elsevier BV - The interaction of the ribotoxin α-sarcin with complex model lipid vesicles
Carmen García-Montoya, Sara García-Linares, Diego Heras-Márquez, Manca Majnik, Diego Laxalde-Fernández, Rafael Amigot-Sánchez, Álvaro Martínez-del-Pozo, and Juan Palacios-Ortega
Elsevier BV - Author Correction: Determination of the boundary lipids of sticholysins using tryptophan quenching (Scientific Reports, (2022), 12, 1, (17328), 10.1038/s41598-022-21750-y)
Juan Palacios‑Ortega, Rafael Amigot‑Sánchez, Carmen García‑Montoya, Ana Gorše, Diego Heras‑Márquez, Sara García‑Linares, Álvaro Martínez‑del‑Pozo, and J. Peter Slotte
Springer Science and Business Media LLC - Sticholysin recognition of ceramide-phosphoethanolamine
Carmen García-Montoya, Diego Heras-Márquez, Rafael Amigot-Sánchez, Sara García-Linares, Álvaro Martínez-del-Pozo, and Juan Palacios-Ortega
Elsevier BV - Determination of the boundary lipids of sticholysins using tryptophan quenching
Juan Palacios-Ortega, Rafael Amigot-Sánchez, Carmen García-Montoya, Ana Gorše, Diego Heras-Márquez, Sara García-Linares, Álvaro Martínez-del-Pozo, and J. Peter Slotte
Springer Science and Business Media LLC
AbstractSticholysins are α-pore-forming toxins produced by the sea-anemone Stichodactyla helianthus. These toxins exert their activity by forming pores on sphingomyelin-containing membranes. Recognition of sphingomyelin by sticholysins is required to start the process of pore formation. Sphingomyelin recognition is coupled with membrane binding and followed by membrane penetration and oligomerization. Many features of these processes are known. However, the extent of contact with each of the different kinds of lipids present in the membrane has received little attention. To delve into this question, we have used a phosphatidylcholine analogue labeled at one of its acyl chains with a doxyl moiety, a known quencher of tryptophan emission. Here we present evidence for the contact of sticholysins with phosphatidylcholine lipids in the sticholysin oligomer, and for how each sticholysin isotoxin is affected differently by the inclusion of cholesterol in the membrane. Furthermore, using phosphatidylcholine analogs that were labeled at different positions of their structure (acyl chains and headgroup) in combination with a variety of sticholysin mutants, we also investigated the depth of the tryptophan residues of sticholysins in the bilayer. Our results indicate that the position of the tryptophan residues relative to the membrane normal is deeper when cholesterol is absent from the membrane. - Sea Anemones, Actinoporins, and Cholesterol
Juan Palacios-Ortega, Diego Heras-Márquez, Rafael Amigot-Sánchez, Carmen García-Montoya, Carlos Torrijos, Diego Laxalde, José G. Gavilanes, Sara García-Linares, and Álvaro Martínez-del-Pozo
MDPI AG
Spanish or Spanish-speaking scientists represent a remarkably populated group within the scientific community studying pore-forming proteins. Some of these scientists, ourselves included, focus on the study of actinoporins, a fascinating group of metamorphic pore-forming proteins produced within the venom of several sea anemones. These toxic proteins can spontaneously transit from a water-soluble fold to an integral membrane ensemble because they specifically recognize sphingomyelin in the membrane. Once they bind to the bilayer, they subsequently oligomerize into a pore that triggers cell-death by osmotic shock. In addition to sphingomyelin, some actinoporins are especially sensible to some other membrane components such as cholesterol. Our group from Universidad Complutense of Madrid has focused greatly on the role played by sterols in this water–membrane transition, a question which still remains only partially solved and constitutes the main core of the article below. - Sticholysin I–II oligomerization in the absence of membranes
Sara García‐Linares, Rafael Amigot‐Sánchez, Carmen García‐Montoya, Diego Heras‐Márquez, Carlos Alfonso, Juan Román Luque‐Ortega, José G. Gavilanes, Álvaro Martínez‐del‐Pozo, and Juan Palacios‐Ortega
Wiley
Sticholysins are pore‐forming toxins produced by the sea anemone Stichodactyla helianthus. When they encounter a sphingomyelin‐containing membrane, these proteins bind to it and oligomerize, a process that ends in pore formation. Mounting evidence indicates that StnII can favour the activity of StnI. Previous results have shown that these two isotoxins can oligomerize together. Furthermore, StnII appeared to potentiate the activity of StnI through the membrane‐binding step of the process. Hence, isotoxin interaction should occur prior to membrane encounter. Here, we have used analytical ultracentrifugation to investigate the oligomerization of Stns in solution, both separately and together. Our results indicate that while StnI seems to be more prone to oligomerize in water solution than StnII, a small percentage of StnII in StnI–StnII mixtures promotes oligomerization.
RECENT SCHOLAR PUBLICATIONS
- The interaction of the ribotoxin α-sarcin with complex model lipid vesicles
C Garca-Montoya, S Garca-Linares, D Heras-Mrquez, M Majnik, ...
Archives of Biochemistry and Biophysics 751, 109836 2024 - Sticholysin recognition of ceramide-phosphoethanolamine
C Garca-Montoya, D Heras-Mrquez, R Amigot-Snchez, ...
Archives of Biochemistry and Biophysics 742, 109623 2023 - Determination of the boundary lipids of sticholysins using tryptophan quenching (vol 12, 17328, 2022)
J Palacios-Ortega, R Amigot-Sanchez, C Garcia-Montoya, A Gorse, ...
SCIENTIFIC REPORTS 13 (1) 2023 - Author Correction: Determination of the boundary lipids of sticholysins using tryptophan quenching
J Palacios‑Ortega, R Amigot‑Snchez, C Garca‑Montoya, A Gorše, ...
Scientific Reports 13, 9343 2023 - Determination of the boundary lipids of sticholysins from tryptophan quenching
J Palacios Ortega, R Amigot Snchez, C Garca Montoya, A Gorse, ...
2022 - Determination of the boundary lipids of sticholysins using tryptophan quenching
J Palacios-Ortega, R Amigot-Snchez, C Garca-Montoya, A Gorše, ...
Scientific reports 12 (1), 17328 2022 - Sea anemones, actinoporins, and cholesterol
J Palacios-Ortega, D Heras-Mrquez, R Amigot-Snchez, ...
International Journal of Molecular Sciences 23 (15), 8771 2022 - Sticholysin I–II oligomerization in the absence of membranes
S Garca‐Linares, R Amigot‐Snchez, C Garca‐Montoya, ...
FEBS letters 596 (8), 1029-1036 2022
MOST CITED SCHOLAR PUBLICATIONS
- Sticholysin recognition of ceramide-phosphoethanolamine
C Garca-Montoya, D Heras-Mrquez, R Amigot-Snchez, ...
Archives of Biochemistry and Biophysics 742, 109623 2023
Citations: 4 - Determination of the boundary lipids of sticholysins using tryptophan quenching
J Palacios-Ortega, R Amigot-Snchez, C Garca-Montoya, A Gorše, ...
Scientific reports 12 (1), 17328 2022
Citations: 3 - Sea anemones, actinoporins, and cholesterol
J Palacios-Ortega, D Heras-Mrquez, R Amigot-Snchez, ...
International Journal of Molecular Sciences 23 (15), 8771 2022
Citations: 3 - Sticholysin I–II oligomerization in the absence of membranes
S Garca‐Linares, R Amigot‐Snchez, C Garca‐Montoya, ...
FEBS letters 596 (8), 1029-1036 2022
Citations: 2
Publications
Palacios-Ortega, Juan et al. “Sea Anemones, Actinoporins, and International journal of molecular sciences vol. 23,15 8771. 7 Aug. 2022, doi:10.3390/ijms23158771.
García-Montoya, Carmen et al. “Sticholysin recognition of Archives of biochemistry and biophysics vol. 742 (2023): 109623. doi:10.1016/j..
García-Linares, Sara et al. “Sticholysin I-II oligomerization in the absence of FEBS letters vol. 596,8 (2022): 1029-1036. doi:10.1002/1873-3468.14326.
Palacios-Ortega, Juan et al. “Author Correction: Determination of the boundary lipids of sticholysins using tryptophan Scientific reports vol. 13,1 9343. 8 Jun. 2023, doi:10.1038/s41598-023-36403-x.
Palacios-Ortega, Juan et al. “Determination of the boundary lipids of sticholysins using tryptophan Scientific reports vol. 12,1 17328. 15 Oct. 2022, doi:10.1038/s41598-022-21750-y.
García-Montoya, Carmen et al. “The interaction of the ribotoxin α-sarcin with complex model lipid Archives of biochemistry and biophysics vol. 751 (2024): 109836. doi:10.1016/j..