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Professor / Departamento de Microbiologia e Parasitologia
Universidade Federal Fluminense
The Journal of parasitology, eISSN: 19372345, Volume: 105, Pages: 248-251, Published: 1 April 2019
Gabriela Cardoso Goes, Karina Costa Coelho Gonçalves, Adriana Pittella Sudré, Danuza Pinheiro Bastos Garcia Mattos, Beatriz Brener, Paula Borba Cruz, Valmir Laurentino Silva, and Patricia Riddell Millar
Journal of Tropical Pathology, ISSN: 03010406, eISSN: 19808178, Pages: 121-133, Published: 2019 Universidade Federal de Goias
The present study evaluated the frequency of intestinal parasitoses in children in public day care centers applying parasitological and immunological diagnostic methods. Fecal samples from 121 children from six public daycare centers were analyzed using parasitological techniques. Epidemiological data were obtained through a questionnaire, where parents and / or guardians were asked, for instance, whether the children had contact with soil, ate raw food, such as vegetables or raw or undercooked meat, normally walked around barefoot or had contact with animals. Fecal samples from 82 children were also tested for Giardia intestinalis and Cryptosporidium sp. coproantigen using the enzyme-linked immunosorbent assay (ELISA) which was also used for Entamoeba coproantigen detection only in samples that tested positive for the parasite by parasitological stool exam/optical microscopy. Intestinal parasite infection was noted in 23.1% (28/121) of the children. The most frequent parasite was Giardia intestinalis (13.2%), followed by Entamoeba coli (5.8%), Blastocystis spp. (1.7%), Endolimax nana (1.7%), Enterobius vermicularis (1.7%), Cystoisospora belli (0.8%),Entamoeba histolytica/E. dispar complex (0.8%), and Ascaris lumbricoides (0.8%). Positivity for parasite infection using parasitological stool exams was significantly associated with age groups, with a higher frequency in 4 to 6 year old children (p=0.03). No association or significant variations were noted in the prevalence of intestinal parasites in relation to the epidemiological variables studied. All samples were negative for Cryptosporidium sp. and Entamoeba histolytica detected by immunological testing, and 17.1% (14/82) children tested positive for Giardia intestinalis, although using parasitological exam/optical microscopy, only 14.6% (12/82) tested positive. The high incidence of intestinal parasites, especially protozoans, suggests probable interpersonal transmission among the children, environmental contamination, or even contaminated food/water intake. Thus, consolidation of preventive measures and efficient diagnostic resources as well as control of intestinal parasites and patient treatment are of utmost importance.
Daniela Leles, Paula Cascardo, Elisa Pucu, Beatriz Brener, Adriana Sudré, Elizabeth Alves, Flávia Uchoa, Priscilla Fajardo, Patrícia Millar, Danuza Mattos, Márcia Chame, and Castor Cartelle
Parasitology International, ISSN: 13835769, eISSN: 18730329, Pages: 776-780, Published: December 2018 Elsevier BV
The use of diagnostic methods that prevent irreplaceable samples (from museum collections, archaeological and paleontological samples) of being consumed or that increase their yield is relevant. For museum collections, archaeological and paleontological samples it is essential to conserve samples, subsamples or portions for future research. We are addressing methods for conservation of irreplaceable samples that could be fully consumed. Innovations in methodologies that are used in studies of Paleoparasitology and Paleomicrobiology will contribute to the preservation of collections. Therefore, to the development of archaeology and paleontology in the future, we evaluated whether the discarded material of the immunochromatography test could be used for molecular diagnosis and vice versa. We used a genotyped experimental coprolite positive for Giardia duodenalis. The diagnosis was positive for giardiasis in both cases. This methodology can be corroborated with the coprolite of a Paleolama maior (extinct llama) previously diagnosed for G. duodenalis with an immunoenzymatic test. The residue of the pre-digestion step of the DNA extraction before adding Proteinase K was confirmed positive with the immunochromatographic test. Also, the DNA extraction residue from a coprolite of Nothrotherium maquinense (ground sloth) was tested positive with immunochromatographic test for G. duodenalis. These are the oldest findings for G. duodenalis confirming that this intestinal parasite occurred among Northeastern Brazilian Megafauna animals from the late Pleistocene period, correlated to human occupation. The relevance of these results will allow the study by different methodological approaches from a small amount of material, reusing discarded materials.
D.P.B.G. Mattos, M.A. Verícimo, L.M.S. Lopes, and S.C. São Clemente
Journal of Helminthology, ISSN: 0022149X, eISSN: 14752697, Pages: 203-207, Published: 8 March 2015 Cambridge University Press (CUP)
AbstractThe aim of this study was to verify the immunogenicity ofPterobothrium heteracanthum(Cestoda: Trypanorhyncha) crude protein extract (PH-CPE) in BALB/c mice. The parasites were obtained fromMicropogonias furnieri(Osteichthyes: Sciaenidae). Groups of six mice were each immunized with 10, 50 or 100 μg of PH-CPE, on days 0 and 35. Both specific IgG and IgE responses were developed after immunization. The immunoblot assay revealed that specific IgG recognizes PH-CPE proteins with two molecular weight ranges, 60–75 and 30–40 kDa, and that IgE recognizes larger proteins over 120 kDa. This appears to be the first report on the immunogenicity of metacestodes within the Pterobothriidae and that PH-CPE is a potential inducer of a specific IgE response.
Revista Brasileira de Medicina Veterinaria, ISSN: 01002430, eISSN: 25272179, Pages: 375-379, Published: 1 October 2014
Patricia Riddell Millar, Fernanda Loureiro de Moura, Otílio Machado Pereira Bastos, Danuza Pinheiro Bastos Garcia de Mattos, Ana Beatriz Monteiro Fonseca, Adriana Pittella Sudré, Daniela Leles, and Maria Regina Reis Amendoeira
Revista do Instituto de Medicina Tropical de Sao Paulo, ISSN: 00364665, eISSN: 16789946, Pages: 433-438, Published: 2014 FapUNIFESP (SciELO)
The present study conducted a toxoplasmosis-related knowledge level survey with 400 pregnant and puerperal women attended in public health units in the municipality of Niterói, Rio de Janeiro. Only 111 (27.8%) women claimed to know about the disease. Most of them (n = 289; 72.2%) had never heard about toxoplasmosis nor knew how to prevent the infection by Toxoplasma gondii. A significant difference (p = 0.013) regarding the presence of anti-T. gondii IgG was observed between women who claimed to know about the disease and those who had never heard about it. These results highlight the importance of a systematic serological screening process for toxoplasmosis, as well as the importance of primary prevention by accurate information during prenatal care, an important Public Health action to be implemented.
Fernanda Loureiro de Moura, Maria Regina Reis Amendoeira, Otílio Machado Pereira Bastos, Danuza Pinheiro Bastos Garcia de Mattos, Ana Beatriz Monteiro Fonseca, José Leonardo Nicolau, Leandro Baptista das Neves, and Patricia Riddell Millar
Revista da Sociedade Brasileira de Medicina Tropical, ISSN: 00378682, Published: March 2013 FapUNIFESP (SciELO)
INTRODUCTION To determine the prevalence of immunoglobulin G (IgG) and immunoglobulin M (IgM) anti-Toxoplasma gondii antibodies among pregnant and postpartum women attended within the public healthcare system in Niterói, State of Rio de Janeiro,and to detect possible exposure factors associated with T. gondii infection in this population. METHODS IgM and IgG anti- T. gondii antibodies were investigated in 276 pregnant and 124 postpartum women by using the indirect immunofluorescence (IFAT) and immunoenzymatic assay (ELISA) techniques. The participants were selected by convenience sampling. All these 400 patients filled out a free and informed consent statement, answered an epidemiological questionnaire and were informed about the disease. RESULTS Among the 400 samples analyzed, 234 (58.5%) were reactive to IgG anti-T. gondii antibodies, according to the IFAT and/or ELISA assay. One pregnant woman was found to be reactive to IgM anti- T. gondii antibodies, with an intermediate IgG avidity test. Risk factor analysis showed that seropositivity was significantly associated (p<0.05) with age, contact with cats and presence of rodents at home. Through a logistic regression model, these associations were confirmed for age and contact with cats, while education at least of the high school level was found to be a protective factor. CONCLUSIONS The prevalence rate of IgG anti-T. gondii antibodies in the city of Niterói was high and the risk factors for infection detected after multivariate analysis were: age over 30 years, contact with cats and education levels lower than university graduate level.
Beatriz Brener, Patricia Riddell Millar, Danuza Pinheiro Bastos Garcia de Mattos, Flávia Uchôa, Bethânia Bastos, Ingrid Rodrigues Lyrio, Pedro Luis Aragon, and Adriana Pittella Sudré
Revista do Instituto de Medicina Tropical de Sao Paulo, ISSN: 00364665, eISSN: 16789946, Pages: 175-177, Published: May 2012 FapUNIFESP (SciELO)
Report of two canine dirofilariosis cases of ectopic location in the state of Rio de Janeiro. This is the first report of erratic migration for this parasitosis in dogs in the state, calling attention to the short period of time between the two cases. The fact that the area is endemic for this parasite, its zoonotic potential and the report of human cases in the state, demonstrates that authorities should be alerted to the control programs of dirofilariosis along with the pathogenic profile of the infections.