Sameer A. M. Abdulrahman
@baydaauniv.net
Associate Professor of Analytical Chemistry, Department of Chemistry, Faculty of Education and Sciences- Rada’a
Albaydha University, Albaydha, Yemen
RESEARCH INTERESTS
Pharmaceutical Analytical Chemistry
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
Sameer A. M. Abdulrahman and Abdullah R. Al-khawlani
Springer Science and Business Media LLC
AbstractThe development and validation of two uncomplicated, selective, and non-extraction-based visible spectrophotometric methods was carried out for the quantification of a tricyclic antidepressant amitriptyline hydrochloride as pharmaceutical tablets as well as in its pure form. The methods rely on the formation of ion pair complexes between amitriptyline base and two acidic dyes, bromothymol blue and bromocresol purple, which were subsequently measured at 410 and 400 nm, respectively. The developed methods included numerous reaction variables that were examined and improved. Beer's law is applicable under optimal conditions for the bromothymol blue and bromocresol purple methods, respectively, over concentrations from 0.5 to 12.5 μg mL−1 and from 0.5 to 10.0 μg mL−1 amitriptyline hydrochloride. The computed values of molar absorptivity were 1.79 × 104 and 2.32 × 104 L.mol−1.cm−1 for bromothymol blue and bromocresol purple methods, respectively, with Sandell’s sensitivity values of 0.0155 and 0.0119 μg cm−2. Both detection limits and quantification limits were computed and determined to be 0.55 and 1.67 μg mL−1 for bromothymol blue method, and 0.49 and 1.49 μg mL−1 for bromocresol purple method, respectively. The developed methods were successfully applied to estimate the concentration of amitriptyline hydrochloride in bulk form and commercial pills. Therefore, these methods demonstrate promising potential for testing this drug in labs for quality control. Graphical abstract Spectrophotometric quantification of amitriptyline hydrochloride in pharmaceutical tablets: method development and validation
Aisha Khan Khanzada, Hussein E. Al-Hazmi, Bogna Śniatała, Tomy Muringayil Joseph, Joanna Majtacz, Sameer A.M. Abdulrahman, Saeed S. Albaseer, Tonni Agustiono Kurniawan, Zohreh Rahimi-Ahar, Sajjad Habibzadeh,et al.
Elsevier BV
Sadeq A. A. Alkhadher, Hussein E. Al-Hazmi, Suhaimi Suratman, Mohamad P. Zakaria, Najat Masood, Bartosz Szeląg, Sami M. Magam, Ebrahim H. H. Al-Qadami, Joanna Majtacz, Przemysław Kowal,et al.
MDPI AG
In this study, the use of linear alkylbenzenes (LABs) was employed to pinpoint the sources of human activity that cause detrimental impacts on the coastal environment and river ecosystems. LABs were detected using GC–MS in sediment samples assembled from Kim Kim River (KKR) and the Port Dickson coast (PDC). To assess the significance of variations in the distribution and concentrations of LABs across the sampling sites, this study utilized several statistical techniques such as post hoc tests, LSD techniques, analysis of variance (ANOVA), and the Pearson correlation coefficient using a significance level of p < 0.05. The degradation levels of LABs and wastewater treatment were assessed in the study using internal congeners (I/E), homologs of C13 and C12, and long-to-short-chain (L/S) ratios. The results revealed that the LAB concentrations varied between 88.3 and 112 ng/g dw in KKR and 119 to 256 ng/g dw in the PDC. Most of the surveyed areas exhibited a substantial count of C13–LABs homologs that displayed a significant difference (p < 0.05). The I/E ratios ranged from 1.7 to 2.0 in KKR and from 2.0 to 4.1 in the PDC, suggesting that the effluents originated from sources associated with the physical phase and biological phase in wastewater treatment systems (WWTSs). The results revealed that the degradation of LABs varied between 34% and 38% in KKR and between 40% and 64% in the PDC. This study underscores the importance of ongoing improvements to WWTSs and emphasizes the potential of LABs as indicators for monitoring wastewater contamination.
Sameer Abdulrahman and Jalal Qazzan
Sociedade Brasileira de Quimica (SBQ)
The objective of this study was to develop two simple and selective visible spectrophotometric methods for the determination of antihistamine drug triprolidine hydrochloride (TRH) in the capsules. The methods were based on the formation of ion-pair complexes between TRH and two dyes, namely, bromocresol green (BCG) and bromophenol blue (BPB). The produced ion-pair complexes were measured at 415 and 410 nm for BCG and BPB methods, respectively. Beer’s law was applicable in the concentration ranges of 2.50-15.0 µg mL-1 TRH for both methods. The molar absorptivity values were found to be 2.12 × 104 and 2.07 × 104 L mol-1 cm-1 for the BCG method and BPB method, respectively, and the Sandell’s sensitivity values were 0.0149 and 0.0152 µg cm-2. The limits of detection and quantification were calculated and found to be 0.29 and 0.86 µg mL-1 for the BCG method and 0.31 and 0.95 µg mL-1 for the BPB method. The methods were applied successfully for TRH determination in bulk drug and in the capsules.
S.A.M. Abdulrahman, F.K. Algethami, N.A.S. Qarah, K. Basavaiah, and E. El-Maaiden
Elsevier BV
Nagib Qarah, Sameer Abdulrahman, Faisal Algethami, Kanakapura Basavaiah, and Ezzouhra El-Maaidenen
Sociedade Brasileira de Quimica (SBQ)
Nagib A. S. Qarah*,a, , Sameer A. M. Abdulrahmanb, Faisal K. Algethami#,c, Kanakapura Basavaiahd and Ezzouhra El-Maaidene Department of Chemistry, Faculty of Education-Zabid, Hodeidah University, Hodeidah, Yemen Department of Chemistry, Faculty of Education and Sciences-Rada’a, Al-Baydha University, Al-Baydha, Yemen Department of Chemistry, Faculty of Science, Imam Mohammad Ibn Saud Islamic University, Riyadh, Saudi Arabia Department of Chemistry, University of Mysore, Manasagangothri, Mysuru-570 006, Karnataka, India Laboratory of Biochemistry and Neurosciences, Department of Biology, University Hassan 1, Settat, Morocco
Nagib Abdullah Salem Qarah, Kanakapura Basavaiah, and Sameer Abdulaziz Mohammed Abdulrahman
FapUNIFESP (SciELO)
Nagib A.S. Qarah, Kanakapura Basavaiah, and Sameer A.M. Abdulrahman
Informa UK Limited
Sameer A.M. Abdulrahman, Okram Zenita Devi, Kanakapura Basavaiah, and Kanakapura B. Vinay
Informa UK Limited
Sameer A.M. Abdulrahman and Kanakapura Basavaiah
Elsevier BV
The present study describes two simple, rapid, selective and cost-effective spectrophotometric methods for the determination of dothiepin hydrochloride (DOTH), an antidepressant drug, in bulk drug and pharmaceutical formulations. The first method (method A) is based on the formation of yellow colored ion-pair complex between DOTH and alizarin red S (ARS) in acid medium which was extracted into dichloromethane and the absorbance was measured at 445 nm. The second method (method B) is based on the breaking of the yellow DOTH–ARS ion-pair complex in alkaline medium followed by the measurement of the violet color free dye at 570 nm. Under the optimized conditions, Beer’s law is obeyed over the concentration ranges of 2.50–55.0 and 1.00–35.0 μg ml−1 DOTH for method A and method B, respectively. The molar absorptivity, Sandell’s sensitivity, detection and quantification limits are also calculated. The methods were validated for intra-day and inter-day accuracy and precision; selectivity and robustness and ruggedness. The proposed methods were applied successfully to the determination of DOTH in pure drug and commercial formulations. The accuracy and reliability of the proposed methods were further established by parallel determination by the official method and also by recovery studies via standard addition technique.
Kanakapura Basavaiah and Sameer A.M. Abdulrahman
Elsevier BV
One titrimetric and two spectrophotometric procedures have been developed for the assay of carbamazepine (CBZ) in bulk drug, formulations and spiked human urine. The methods are based on the bromination of CBZ by the bromine generated in situ by the action of the acid on the bromate–bromide mixture. The twin advantages of avoiding liquid bromine and analysis in a cost-effective manner are realized. In titrimetry, the drug was treated with a known excess of bromate–bromide mixture in hydrochloric acid medium followed by the determination of unreacted bromine iodometrically. Spectrophotometry involves the addition of a measured excess of bromate–bromide reagent in acid medium to CBZ, and after the reaction is ensured to be complete, the residual bromine was determined by reacting with a fixed amount of either methyl orange and measuring the absorbance at 510 nm (method A) or indigo carmine and measuring the absorbance at 610 nm (method B). Titrimetric procedure is applicable over the range of 1.00–7.50 mg CBZ, and the calculations are based on a 1:1 reaction stoichiometry (CBZ:KBrO3). In spectrophotometric methods, Beer’s law is valid within concentration ranges of 0.25–1.50 and 0.50–6.00 μg ml−1 CBZ for methods A and B, respectively. The proposed methods were successfully applied to the determination of CBZ in tablets and syrup, in addition to spiked human urine by the spectrophotometric methods, with mean recoveries of 95.50–104.0% and the results were statistically compared with those of an official method by applying Student’s t-test and F-test.
M. S. Raghu, K. Basavaiah, Sameer A. M. Abdulrahaman, K. N. Prashanth, and K. B. Vinay
Pleiades Publishing Ltd
Sameer A. M. Abdulrahman, K. Basavaiah, M. X. Cijo, and K. B. Vinay
Springer Science and Business Media LLC
Spectrophotometric methods have been developed for the determination of dothiepin hydrochloride (DOTH) in both pure and tablet dosage form and their limits of detection and quantification have been evaluated. The methods are based on the measurement of the absorbance of a DOTH solution either in 0.1 N HCl at 229 nm (method A) or in methanol at 231 nm (method B). Beer’s law is obeyed over a concentration range of 1–16 μg/ml DOTH for both methods. Molar absorptivity values are calculated to be 2.48 × 104 and 2.42 × 104 l/(mol × cm) with Sandell sensitivity values of 0.0134 and 0.0137 μg/cm2 for methods A and B, respectively. The degradation behavior of DOTH was investigated under different stress conditions such as acid hydrolysis, alkaline hydrolysis, water hydrolysis, oxidation, dry heat treatment, and UV-degradation. The drug undergoes significant degradation under oxidative conditions only.
K. B. Vinay, H. D. Revanasiddappa, M. S. Raghu, Sameer. A. M. Abdulrahman, and N. Rajendraprasad
Hindawi Limited
Two simple, selective, and rapid spectrophotometric methods are described for the determination of mycophenolate mofetil (MPM) in pure form and in tablets. Both methods are based on charge-transfer complexation reaction of MPM with p-chloranilic acid (p-CA) or 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (DDQ) in dioxane-acetonitrile medium resulting in coloured product measurable at 520 nm (p-CA) or 580 nm (DDQ). Beer’s law is obeyed over the concentration ranges of 40–400 and 12–120 μg mL−1MPM for p-CA and DDQ, respectively, with correlation coefficients (r) of 0.9995 and 0.9947. The apparent molar absorptivity values are calculated to be1.06×103and3.87×103 L mol−1 cm−1, respectively, and the corresponding Sandell’s sensitivities are 0.4106 and 0.1119 μg cm−1. The limits of detection (LOD) and quantification (LOQ) are also reported for both methods. The described methods were successfully applied to the determination of MPM in tablets. Statistical comparison of the results with those of the reference method showed excellent agreement. No interference was observed from the common excipients present in tablets. Both methods were validated statistically for accuracy and precision. The accuracy and reliability of the methods were further ascertained by recovery studiesviastandard addition procedure.
Nagaraj Prashanth, Kanakapura Basavaiah, Sameer Abdulrahman, Nagaraju Rajendraprasad, and Basavaiah Vinay
National Library of Serbia
Two highly sensitive spectrophotometric methods are proposed for the quantification of atenolol (ATN) in pure drug as well as in pharmaceutical formulations. The methods are based on the bromination reaction of ATN with a known excess of bromate-bromide mixture in acid medium followed by the determination of unreacted bromine. The residual bromine is determined by its reaction with excess iodide and the liberated iodine (I3?) is either measured at 360 nm (method A) or reacted with starch followed by the measurement of the starch-iodine chromogen at 570 nm (method B). Under the optimum conditions, ATN could be assayed in the concentration ranges of 0.5-9.0 and 0.3-6.0?g mL-1 for method A and method B, respectively, with corresponding molar absorptivity values of 2.36?104 and 2.89?104 L/mol.cm. Sandell?s sensitivity values are found to be 0.0113 and 0.0092 ?g/cm2 for method A and method B, respectively. The proposed methods were successfully applied to the analysis of different commercial brands of pharmaceutical formulations and the results obtained by the proposed methods were in good agreement with those obtained using the reference method. The reliability of the methods was further ascertained by recovery studies using standard- addition method.
M. S. Raghu, K. Basavaiah, P. J. Ramesh, Sameer A. M. Abdulrahman, and K. B. Vinay
Springer Science and Business Media LLC
A sensitive, precise, and cost-effective UV-spectrophotometric method is described for the determination of pheniramine maleate (PAM) in bulk drug and tablets. The method is based on the measurement of absorbance of a PAM solution in 0.1 N HCl at 264 nm. As per the International Conference on Harmonization (ICH) guidelines, the method was validated for linearity, accuracy, precision, limits of detection (LOD) and quantification (LOQ), and robustness and ruggedness. A linear relationship between absorbance and concentration of PAM in the range of 2–40 μg/ml with a correlation coefficient (r) of 0.9998 was obtained. The LOD and LOQ values were found to be 0.18 and 0.39 μg/ml PAM, respectively. The precision of the method was satisfactory: the value of relative standard deviation (RSD) did not exceed 3.47%. The proposed method was applied successfully to the determination of PAM in tablets with good accuracy and precision. Percentages of the label claims ranged from 101.8 to 102.01% with the standard deviation (SD) from 0.64 to 0.72%. The accuracy of the method was further ascertained by recovery studies via a standard addition procedure. In addition, the forced degradation of PAM was conducted in accordance with the ICH guidelines. Acidic and basic hydrolysis, thermal stress, peroxide, and photolytic degradation were used to assess the stability-indicating power of the method. A substantial degradation was observed during oxidative and alkaline degradations. No degradation was observed under other stress conditions.
Sameer Abdulrahman and Kanakapura Basavaiah
National Library of Serbia
Two simple, sensitive and extraction-free spectrophotometric methods are described for the determination of dothiepin hydrochloride (DOTH) both in pure form and in pharmaceutical tablets. The methods are based on ion-pair complex formation between dothiepin base (DOT) and two acidic dyes, namely, bromophenol blue (BPB) or bromocresol green (BCG) with absorption maximum at 425 nm for BPB method or 430 nm for BCG method. Beer?s law is obeyed over the concentration ranges of 1.0-15.0 and 1.0-17.5 ?g mL-1 DOT for BPB and BCG methods, respectively. The molar absorptivity values and Sandell?s sensitivity values are reported for both methods. The limits of detection (LOD) and quantification (LOQ) were calculated to be 0.18 and 0.53 ?g mL-1 for BPB method, and 0.17 and 0.50 ?g mL-1 for BCG method, respectively. The stoichiometry of the complex in either case was found to be 1: 1 and the conditional stability constant (KF) of the complexes has also been calculated. The proposed methods were applied successfully to the determination of DOTH in pure form and in its tablet form with good accuracy and precision. Statistical comparison of the results was performed using Student's t-test and variance ratio F-test at 95% confidence level and there was no significant difference between the official and proposed methods with regard to accuracy and precision. Further, the validity of the proposed methods was confirmed by recovery studies via standard addition technique.
Kanakapura Basavaiah and Sameer A. M. Abdulrahman
Wiley
This is the first report on the use of visible spectrophotometry for the determination of bupropion hydrochloride (BUPH), a second-generation antidepressant, in pharmaceuticals. Two sensitive, selective, and cost-effective spectrophotometric methods are described. The first method (method A) is based on the formation of yellow-coloured ion-pair complex between the BUPH and methyl orange (MO) at pH 3.80 ± 0.10 which was extracted into dichloromethane and the absorbance measured at 425 nm. The second method (method B) is based on the breaking of the yellow BUPH-MO ion-pair complex in acid medium followed by the measurement of the red-pink colour at 520 nm. Beer's Law is obeyed over the concentration ranges of 1.00-12.0 and 0.48-7.20 µg ml(-1) BUPH for method A and method B, respectively. The molar absorptivities are calculated to be 2.18 × 10(4) and 3.79 × 10(4) l mol(-1) cm(-1) for method A and method B, respectively, and the corresponding Sandell sensitivity values are 0.0127 and 0.0073 µg cm(-2) . The limits of detection and quantification have also been reported. The proposed methods were applied successfully to the determination of BUPH in pure drug and commercial tablets. The accuracy and reliability of the proposed methods were further ascertained by recovery studies via standard addition technique.
Sameer Abdulrahman and Kanakapura Basavaiah
National Library of Serbia
Two simple and rapid titrimetric methods are described for the determination of bupropion hydrochloride (BUPH) in pharmaceuticals. The proposed methods are based on the solvent extraction-titration of BUPH with two ion-association reagents, i.e. sodium lauryl sulphate (SLS) and tetraphenylborate (TPB). In method A, SLS was used as titrant and the titration was carried out in the presence of dilute sulphuric acid and chloroform using dimethyl yellow as indicator whereas in method B, the titrant was TPB and the titration was done in borate-phosphate buffer of pH 6.0 and 1,2-dichloroethane with tetrabromophenolphthalein ethyl ester (TBPE) as indicator. In the two-phase titrations, the proposed procedures give sharp end points as the color of the organic phase changes from yellow to pink in method A and from red-violet to yellow in method B. The methods are applicable over the ranges of 1.0-20.0 and 3.0-10.0 mg of BUPH for method A and method B, respectively. The accuracy and precision of the methods are good. The methods were applied successfully to the determination of BUPH in tablets and the results were in agreement with the label claim and those of the comparison method.