Pradipta Ranjan Rauta

Scopus Publications

Eco-Friendly Mycogenic Synthesis of Iron Oxide Nanoparticles Using Volvariella volvacea and Unveiling its Potential as Antibiofilm and Anticancer Agents
Debasis Nayak, Awdhesh Kumar Mishra, Sasmita Malla, Umakanta Swain, Kalpana Panigrahi, Jibanjyoti Panda, Sarvesh Rustagi, Pradipta Ranjan Rauta, and Yugal Kishore Mohanta
The Electrochemical Society
This research explores the eco-friendly creation of functional, biocompatible nanomaterials through green chemistry, utilizing natural extracts over chemicals to produce metal and metal oxide nanoparticles. The study focuses on iron oxide nanoparticles synthesized with Volvariella volvacea (GIONP) and evaluates their physical, chemical, and biological traits, including their anticancer capabilities, against traditionally made IONPs (CIONP). GIONPs, confirmed by UV-visible spectroscopy and dynamic light scattering, showed enhanced stability and consistent size distribution. They also demonstrated smaller sizes via -Field Emission scanning electron microscopy analysis, and X-ray diffraction and Fourier transform infrared studies verified their crystalline structure and organic functional groups. Notably, GIONPs exhibited strong antioxidant properties, low toxicity to blood cells, promising for intravenous use, and greater antimicrobial and antibiofilm activities against various bacterial strains than CIONPs. The study revealed that both GIONPs and CIONPs exhibit a similar degree of toxicity towards MDA-MB 231 cancer cells. The internalization of GIONPs within these cells was verified through a Prussian blue assay and further supported by the concentration-dependent increase in intracellular iron levels post-treatment. These findings underscore the successful formulation of GIONPs that are not only biocompatible and eco-friendly but also capped with bioactive compounds, highlighting their promising applications in the field of biomedicine.

Exploring Biopolymer for Food and Pharmaceuticals Application in the Circular Bioeconomy: An Agro-Food Waste-to-Wealth Approach
Jibanjyoti Panda, Awdhesh Kumar Mishra, Yugal Kishore Mohanta, Kaustuvmani Patowary, Pradipta Ranjan Rauta, and Bishwambhar Mishra
Springer Science and Business Media LLC

In Vitro and In Silico Characterization of Curcumin-Loaded Chitosan–PVA Hydrogels: Antimicrobial and Potential Wound Healing Activity
Hitesh Chopra, Shabana Bibi, Yugal Kishore Mohanta, Tapan Kumar Mohanta, Sandeep Kumar, Inderbir Singh, Muhammad Saad Khan, Pradipta Ranjan Rauta, Abdulrahman Alshammari, Metab Alharbi,et al.
MDPI AG
Curcumin has been used in traditional medicine forages. The present study aimed to develop a curcumin-based hydrogel system and assess its antimicrobial potential and wound healing (WH) activity on an invitro and in silico basis. A topical hydrogel was prepared using chitosan, PVA, and Curcumin in varied ratios, and hydrogels were evaluated for physicochemical properties. The hydrogel showed antimicrobial activity against both gram-positive and gram-negative microorganisms. In silico studies showed good binding energy scores and significant interaction of curcumin components with key residues of inflammatory proteins that help in WH activity. Dissolution studies showed sustained release of curcumin. Overall, the results indicated wound healing potential of chitosan–PVA–curcumin hydrogel films. Further in vivo experiments are needed to evaluate the clinical efficacy of such films for wound healing.

An Insight into Advances in Developing Nanotechnology Based Therapeutics, Drug Delivery, Diagnostics and Vaccines: Multidimensional Applications in Tuberculosis Disease Management
Hitesh Chopra, Yugal Kishore Mohanta, Pradipta Ranjan Rauta, Ramzan Ahmed, Saurov Mahanta, Piyush Kumar Mishra, Paramjot Panda, Ali A. Rabaan, Ahmad A. Alshehri, Basim Othman,et al.
MDPI AG
Tuberculosis (TB), one of the deadliest contagious diseases, is a major concern worldwide. Long-term treatment, a high pill burden, limited compliance, and strict administration schedules are all variables that contribute to the development of MDR and XDR tuberculosis patients. The rise of multidrug-resistant strains and a scarcity of anti-TB medications pose a threat to TB control in the future. As a result, a strong and effective system is required to overcome technological limitations and improve the efficacy of therapeutic medications, which is still a huge problem for pharmacological technology. Nanotechnology offers an interesting opportunity for accurate identification of mycobacterial strains and improved medication treatment possibilities for tuberculosis. Nano medicine in tuberculosis is an emerging research field that provides the possibility of efficient medication delivery using nanoparticles and a decrease in drug dosages and adverse effects to boost patient compliance with therapy and recovery. Due to their fascinating characteristics, this strategy is useful in overcoming the abnormalities associated with traditional therapy and leads to some optimization of the therapeutic impact. It also decreases the dosing frequency and eliminates the problem of low compliance. To develop modern diagnosis techniques, upgraded treatment, and possible prevention of tuberculosis, the nanoparticle-based tests have demonstrated considerable advances. The literature search was conducted using Scopus, PubMed, Google Scholar, and Elsevier databases only. This article examines the possibility of employing nanotechnology for TB diagnosis, nanotechnology-based medicine delivery systems, and prevention for the successful elimination of TB illnesses.

Pancreatic tumor microenvironmental acidosis and hypoxia transform gold nanorods into cell-penetrant particles for potent radiosensitization
Pradipta Ranjan Rauta, Yuri Mackeyev, Keith Sanders, Joseph B.K. Kim, Valeria V. Gonzalez, Yasmin Zahra, Muhammad A. Shohayeb, Belal Abousaida, Geraldine V. Vijay, Okan Tezcan,et al.
American Association for the Advancement of Science (AAAS)
Coating nanoparticles with stealth epilayers increases circulation time by evading opsonization, macrophage phagocytosis, and reticuloendothelial sequestration. However, this also reduces internalization by cancer cells upon reaching the tumor. We designed gold nanorods (GNRs) with an epilayer that retains stealth properties in circulation but transforms spontaneously in the acidotic tumor microenvironment to a cell-penetrating particle. We used a customized stoichiometric ratio of l -glutamic acid and l -lysine within an amphiphilic polymer of poly( l -glutamic acid-co- l -lysine), or P(Glu-co-Lys), to effect this transformation in acidotic environments. P(Glu-co-Lys)-GNRs were internalized by cancer cells to facilitate potent in vitro radiosensitization. When administered intravenously in mice, they accumulate in the periphery and core of tumors without any signs of serum biochemical or hematological alterations, normal organ histopathological abnormalities, or overt deterioration in animal health. Furthermore, P(Glu-co-Lys)-GNRs penetrated the tumor microenvironment to accumulate in the hypoxic cores of tumors to potently radiosensitize heterotopic and orthotopic pancreatic cancers in vivo.

Ethosomes for Dermal and Transdermal Drug Delivery Systems
Debasis Nayak, Yugal Kishore Mohanta, Pradipta Ranjan Rauta, Ishani Chakrabartty, and Muthupandian Saravanan
Springer International Publishing

Community-based asthma assessment in young children: adaptations for a multicentre longitudinal study in South Asia
Mohammad Shahidul Islam, Samin Huq, Steven Cunningham, Jurgen Schwarze, A.S.M.D. Ashraful Islam, Mashal Amin, Farrukh Raza, Radanath Satpathy, Pradipta Ranjan Rauta, Salahuddin Ahmed,et al.
SAGE Publications
Background: Systematic assessment of childhood asthma is challenging in low- and middle-income country (LMIC) settings due to the lack of standardised and validated methodologies. We describe the contextual challenges and adaptation strategies in the implementation of a community-based asthma assessment in four resource-constrained settings in Bangladesh, India, and Pakistan. Method: We followed a group of children of age 6–8 years for 12 months to record their respiratory health outcomes. The study participants were enrolled at four study sites of the ‘Aetiology of Neonatal Infection in South Asia (ANISA)’ study. We standardised the research methods for the sites, trained field staff for uniform data collection and provided a ‘Child Card’ to the caregiver to record the illness history of the participants. We visited the children on three different occasions to collect data on respiratory-related illnesses. The lung function of the children was assessed in the outreach clinics using portable spirometers before and after 6-minute exercise, and capillary blood was examined under light microscopes to determine eosinophil levels. Results: We enrolled 1512 children, 95.5% (1476/1512) of them completed the follow-up, and 81.5% (1232/1512) participants attended the lung function assessment tests. Pre- and post-exercise spirometry was performed successfully in 88.6% (1091/1232) and 85.7% (1056/1232) of children who attempted these tests. Limited access to health care services, shortage of skilled human resources, and cultural diversity were the main challenges in adopting uniform procedures across all sites. Designing the study implementation plan based on the local contexts and providing extensive training of the healthcare workers helped us to overcome these challenges. Conclusion: This study can be seen as a large-scale feasibility assessment of applying spirometry and exercise challenge tests in community settings of LMICs and provides confidence to build capacity to evaluate children’s respiratory outcomes in future translational research studies.

Exploring the Bioactive Potentials of C<inf>60</inf>-AgNPs Nano-Composites against Malignancies and Microbial Infections
Kunal Biswas, Awdhesh Kumar Mishra, Pradipta Ranjan Rauta, Abdullah G. Al-Sehemi, Mehboobali Pannipara, Avik Sett, Amra Bratovcic, Satya Kumar Avula, Tapan Kumar Mohanta, Muthupandian Saravanan,et al.
MDPI AG
At present, the potential role of the AgNPs/endo-fullerene molecule metal nano-composite has been evaluated over the biosystems in-vitro. The intra-atomic configuration of the fullerene molecule (C60) has been studied in-vitro for the anti-proliferative activity of human breast adenocarcinoma (MDA-MB-231) cell lines and antimicrobial activity against a few human pathogens that have been augmented with the pristine surface plasmonic electrons and antibiotic activity of AgNPs. Furthermore, FTIR revealed the basic vibrational signatures at ~3300 cm−1, 1023 cm−1, 1400 cm−1 for O-H, C-O, and C-H groups, respectively, for the carbon and oxygen atoms of the C60 molecule. NMR studies exhibited the different footprints and magnetic moments at ~7.285 ppm, explaining the unique underlying electrochemical attributes of the fullerene molecule. Such unique electronic and physico-chemical properties of the caged carbon structure raise hope for applications into the drug delivery domain. The in-vitro dose-dependent application of C60 elicits a toxic response against both the breast adenocarcinoma cell lines and pathogenic microbes. That enables the use of AgNPs decorated C60 endo fullerene molecules to design an effective anti-cancerous drug delivery and antimicrobial agent in the future, bringing a revolutionary change in the perspective of a treatment regime.

Development of Graphene Oxide Nanosheets as Potential Biomaterials in Cancer Therapeutics: An In-Vitro Study Against Breast Cancer Cell Line
Yugal Kishore Mohanta, Kunal Biswas, Pradipta Ranjan Rauta, Awdhesh Kumar Mishra, Debashis De, Abeer Hashem, Al-Bandari Fahad Al-Arjani, Abdulaziz A. Alqarawi, Elsayed Fathi Abd-Allah, Saurov Mahanta,et al.
Springer Science and Business Media LLC

The application of nanotechnology in enhancing immunotherapy for cancer treatment: Current effects and perspective
Yongjiang Li, Ciceron Ayala-Orozco, Pradipta Ranjan Rauta, and Sunil Krishnan
Royal Society of Chemistry (RSC)
Cancer immunotherapy is emerging as a promising treatment modality that suppresses and eliminates tumors by re-activating and maintaining the tumor-immune cycle, and further enhancing the body's anti-tumor immune response.

Gold nanoparticle-based rapid detection and isolation of cells using ligand-receptor chemistry
Pradipta Ranjan Rauta, Pavan M. Hallur, and Aditya Chaubey
Springer Science and Business Media LLC
AbstractIdentification and isolation of low-frequency cells of interest from a heterogeneous cell mixture is an important aspect of many diagnostic applications (including enumeration of circulating tumor cells) and is integral to various assays in (cancer) biology. Current techniques typically require expensive instrumentation and are not amenable to high throughput. Here, we demonstrate a simple and effective platform for cell detection and isolation using gold nanoparticles (Au NPs) conjugated with hyaluronic acid (HA) i.e. Au-PEG-HA NPs. The proposed platform exploits ligand-receptor chemistry to detect/isolate cells with high specificity and efficiency. When the Au-PEG-HA NPs come in contact with cells that express CD44 (the receptor for HA), a clear colorimetric change occurs (along with an accompanying SPR peak shift from 521 nm to 559 nm) in the solution due to NPs-cell interaction. This clearly discernible, colorimetric change can be leveraged by point-of-care devices employed in diagnostic applications. Finally, we show that we can successfully isolate viable cells from a heterogeneous cell population (including from human blood samples) with high specificity, which can be used in further downstream applications. The developed NPs-based platform can be a convenient and cost-efficient alternative for diagnostic applications and for cell isolation or sorting in research laboratories.

Development, formulation, and evaluation of sodium alginate-g-poly (Acryl amide-co-acrylic acid/cloiste-30B)/agnps hydrogel composites and their applications in paclitaxel drug delivery and anticancer activity
B. H. Nanjunda Reddy, Pradipta Ranjan Rauta, V. Venkata Lakshmi, and Swamy Sreenivasa
Innovare Academic Sciences Pvt Ltd
Objective: The objective of this study was to develop, formulate and evaluate the sodium alginate grafted poly (acrylamide-co-acrylic acid/cloisite-30B/silver nanoparticle hydrogel composites (SA-PAAm-PAAc/C30B/AgNPs) with varying weight percentage (wt %) of cloisite-30B clay for paclitaxel targeted delivery and anticancer activity. Methods: Polymer hydrogel composites of different wt % of cloisite-30B modified clay dispersed sodium alginate (SA) grafted polyacrylamide-co-polyacrylic acid were prepared via in situ free radical initiation polymerization reaction technique. In vitro release of paclitaxel (PT) anticancer drug and anticancer studies were performed. The formulations were further evaluated for swelling, drug encapsulation, drug delivery, anticancer activity study, Fourier transforms infrared spectroscopy (FT-IR), thermogravimetric (TGA), differential scanning calorimeter (DSC) and x-ray diffraction (XRD) characterizations. Results: FT-IR spectroscopy of various composite hydrogel formulations displayed good compatibility between sodium alginate, polyacrylamide, and polyacrylic acid polymers. The thermal study reveals that the formulations with clay (C30B) and AgNPs in hydrogel composites exhibit good thermal stability and less % of weight loss (wt. loss) compared to pure formulations. Further, the highest encapsulation efficiency was shown by the formulation S0-0+D (72.66Â±5.92%) and least encapsulation efficiency was shown by S75Ag+D (41.33Â±3.12%) compared to rest of the formulations and S50Ag+D and S75Ag+D samples exhibits relatively slightly higher and sustained cumulative release rate of PT drug at an average rate of 80Â±9 % within 72 h and also shows relatively better anticancer activity compared to other formulations. Conclusion: Formulations S50Ag+D and S75Ag+Dwere found to be best formulations with a higher cumulative percentage of PT drug release and showed better anticancer activity

Synthesis and characterization of cloisite-30B clay dispersed poly (acryl amide/sodium alginate)/AgNp hydrogel composites for the study of BSA protein drug delivery and antibacterial activity
B H Nanjunda Reddy, Pradipta Ranjan Rauta, V Venkatalakshimi, and Swamy Sreenivasa
IOP Publishing
The aim of this research is to inspect the effect of Cloisite-30B (C30B) modified clay dispersed poly (acrylamide-co-Sodiumalginate)/AgNp hydrogel nanocomposites (PASA/C30B/Ag) for drug delivery and antibacterial activity. A novel hydrogel composite based sodium alginate (SA) and the inorganic modified clay with silver nano particle (C30B/AgNps)polymer hydrogel composites are synthesized via the graft copolymerization of acrylamide (AAm) in an aqueous medium with methylene bisacrylamide (MBA) as a crosslinking agent and ammonium per sulfate(APS) as an initiator. The UV/Visible spectroscopy of obtained composites is successfully studied, which confirms the occurrence of AgNps in the hydrogel composites. And the swelling capacity and bovine serum albumin (BSA) protein as model drug delivery study for these hydrogel nanocomposites have been carried out. The C30B/Ag filled hydrogel composites exhibit superior water absorbency or swelling capacity compared to pure samples and it is establish that the formulations with clay (C30B) dispersed silver nanocomposite hydrogels show improved and somewhat faster rate of drug delivery than other formulations(pure systems) and SEM and TEM reports suggests that the size of AgNps in the composite hydrogels is in the range of 5–10 nm with shrunken surface and the antibacterial characterizations for gram positive and gram negative bacteria are carried out by using Streptococcus faecalis (S. Faecalis) and Escherichia coli (E.coli) as model bacteria and the hydrogel composites of PASA/C30B/Ag shows exceptional antibacterial activity against both the bacteria as compared to pure hydrogel composites samples.

Novel chromogenic bacteria characterized and their probable treatment options using herbal products and reagents to restrict biofilm formation
Sarbani Ashe, Subhadarshani Agasti, Satish Lakkoji, Pradipta Ranjan Rauta, Harekrushna Sahoo, Monalisa Mishra, and Bismita Nayak
University of South Bohemia in Ceske Budejovice

Assessment of antioxidant, antimicrobial and anti-osteosarcoma potential of four traditionally used Indian medicinal plants
Debasis Nayak, Sarbani Ashe, Pradipta Ranjan Rauta, and Bismita Nayak
University of South Bohemia in Ceske Budejovice

Design and characterization of plasmids encoding antigenic peptides of Aha1 from Aeromonas hydrophila as prospective fish vaccines
Pradipta R. Rauta, Bismita Nayak, Gabriel A. Monteiro, and Marília Mateus
Elsevier BV

In silico identification of outer membrane protein (Omp) and subunit vaccine design against pathogenic Vibrio cholerae
Pradipta Ranjan Rauta, Sarbani Ashe, Debasis Nayak, and Bismita Nayak
Elsevier BV

Development of highly biocompatible Gelatin & i-Carrageenan based composite hydrogels: In depth physiochemical analysis for biomedical applications
Jyostna Rani Padhi, Debasis Nayak, Arpita Nanda, Pradipta Ranjan Rauta, Sarbani Ashe, and Bismita Nayak
Elsevier BV

Fabrication of poly(vinyl alcohol)-Carrageenan scaffolds for cryopreservation: Effect of composition on cell viability
Pankaj Chopra, Debasis Nayak, Arpita Nanda, Sarbani Ashe, Pradipta Ranjan Rauta, and Bismita Nayak
Elsevier BV

Enhanced efficacy of clindamycin hydrochloride encapsulated in PLA/PLGA based nanoparticle system for oral delivery
Pradipta Ranjan Rauta, Niladri Mohan Das, Debasis Nayak, Sarbani Ashe, and Bismita Nayak
Institution of Engineering and Technology (IET)
Clindamycin hydrochloride (CLH) is a clinically important oral antibiotic with wide spectrum of antimicrobial activity that includes gram-positive aerobes (staphylococci, streptococci etc.), most anaerobic bacteria, Chlamydia and certain protozoa. The current study was focused to develop a stabilised clindamycin encapsulated poly lactic acid (PLA)/poly (D,L-lactide-co-glycolide) (PLGA) nano-formulation with better drug bioavailability at molecular level. Various nanoparticle (NPs) formulations of PLA and PLGA loaded with CLH were prepared by solvent evaporation method varying drug: polymer concentration (1:20, 1:10 and 1:5) and characterised (size, encapsulation efficiency, drug loading, scanning electron microscope, differential scanning calorimetry [DSC] and Fourier transform infrared [FTIR] studies). The ratio 1:10 was found to be optimal for a monodispersed and stable nano formulation for both the polymers. NP formulations demonstrated a significant controlled release profile extended up to 144 h (both CLH-PLA and CLH-PLGA). The thermal behaviour (DSC) studies confirmed the molecular dispersion of the drug within the system. The FTIR studies revealed the intactness as well as unaltered structure of drug. The CLH-PLA NPs showed enhanced antimicrobial activity against two pathogenic bacteria Streptococcus faecalis and Bacillus cereus. The results notably suggest that encapsulation of CLH into PLA/PLGA significantly increases the bioavailability of the drug and due to this enhanced drug activity; it can be widely applied for number of therapies.

Synergistic combination of antioxidants, silver nanoparticles and chitosan in a nanoparticle based formulation: Characterization and cytotoxic effect on MCF-7 breast cancer cell lines
Debasis Nayak, Aliva Prity Minz, Sarbani Ashe, Pradipta Ranjan Rauta, Manisha Kumari, Pankaj Chopra, and Bismita Nayak
Elsevier BV

Bark extract mediated green synthesis of silver nanoparticles: Evaluation of antimicrobial activity and antiproliferative response against osteosarcoma
Debasis Nayak, Sarbani Ashe, Pradipta Ranjan Rauta, Manisha Kumari, and Bismita Nayak
Elsevier BV

Biologically synthesised silver nanoparticles from three diverse family of plant extracts and their anticancer activity against epidermoid A431 carcinoma
Debasis Nayak, Sonali Pradhan, Sarbani Ashe, Pradipta Ranjan Rauta, and Bismita Nayak
Elsevier BV

Biosynthesis, characterisation and antimicrobial activity of silver nanoparticles using Hibiscus rosa-sinensis petals extracts
Debasis Nayak, Sarbani Ashe, Pradipta Ranjan Rauta, and Bismita Nayak
Institution of Engineering and Technology (IET)
Green synthesis of metallic nanoparticles has lured the world from the chemical and physical approaches owing to its rapid, non-hazardous and economic aspect of production mechanism. In this study, silver nanoparticles (AgNPs) were synthesised using petal extracts of Hibiscus rosa-sinensis. The AgNPs displayed characteristic surface plasmon resonance peak at around 421 nm having a mean particle size of 76.25±0.17 nm and carried a charge of -41±0.2 mV. The X-ray diffraction patterns displayed typical peaks of face centred cubic crystalline silver. The surface morphology was characterised by scanning electron microscopy and atomic force microscopy. Fourier transform infrared spectroscopy studies confirmed the surface modifications of the functional groups for the synthesis of AgNPs. Furthermore, the synthesised AgNPs displayed proficient antimicrobial activity against pathogenic strains of Vibrio cholerae, Escherichia coli, Klebsiella pneumoniae and Staphylococcus aureus.

Parenteral immunization of PLA/PLGA nanoparticle encapsulating outer membrane protein (Omp) from Aeromonas hydrophila: Evaluation of immunostimulatory action in Labeo rohita (rohu)
Pradipta Ranjan Rauta and Bismita Nayak
Elsevier BV

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