Andre Romero da Silva

Graduate at Bachelor of Technological Chemical from Universidade Estadual de Campinas (2000), master's at Chemistry from Universidade Estadual de Campinas (2003) and Ph.D. at Chemistry from Universidade Estadual de Campinas (2007). Has experience in Chemistry, acting on the following subjects: indium meso-tetraphenylporphyrin, photodynamic therapy, photosensitizers, biomolecules photooxidation and mechanism of photooxidation. He has held positions as Chemistry course coordinator, general teaching coordinator, Teaching Director, Director of Research and Pos-Graduate and is the actual Pro-rector of Research and Pos-Graduate at Federal Institute of Espírito Santo.

EDUCATION

2017-2018 - Specialization in Pedagogical Practices by Federal Institute of Espírito Santo - Brazil
2003-2007 - Doctor of Science (Physical Chemistry) by State University of Campinas - Brazil
2001-2003 - Master in Chemistry (Physical Chemistry) by State University of Campinas - Brazil
1995-2000 - Bachelor of Technological Chemistry by State University of Campinas - Brazil

RESEARCH INTERESTS

Nanotechnology, Drug Delivery System, Photodynamic therapy, Factorial Design, Biochemistry, Genetics and Molecular Biology, Chemistry, Coated Fertilizer

FUTURE PROJECTS

Scopus Publications

Magnetite Nanoparticles Encapsulated with PBS-PEG for AMF Hyperthermia
Carlos Augusto Zanoni Souto, Fernando Gomes de Souza Junior, André Romero da Silva, and Kaushik Pal
MDPI AG
Novel studies on typical synthesized magnetite nanoparticles were encapsulated into a poly (butylene succinate)/poly (ethylene glycol) copolymer (PBS-PEG). PBS was chosen because of its biocompatibility characteristics necessary for biomedical applications. PEG, as part of the macromolecular structure, increases the hybrid system’s solubility in an aqueous environment, increasing the circulation time of the material in the bloodstream. The immune system has difficulty recognizing particles with good solubility in an aqueous medium and with a diameter until 200 nm, preventing the body from eliminating the nanoparticles before the magnetic hyperthermia is performed. All the prepared materials were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), X-ray powder diffraction (XRD), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), dynamic light scattering (DLS), and zeta potential. The detailed investigated result executes the formulation developed in this work, showing it has potential and that further studies and analyses can be carried out so that the formulation can be improved, thus obtaining even better results.


PLGA nanoparticles for treatment of cardiovascular diseases
Julyana Noval de Souza Ferreira, Vannyla Viktória Viana Vasconcelos, Barbara Silva Figueiredo, Demian Paiva Alves, Antony Luca Luna Vieira de Abreu, Priscila Ponate de Souza, Dayana Laura Nascimento Costa, and André Romero da Silva
Elsevier



Interaction effects between macromolecules and photosensitizer on the ability of AlPc and InPc-loaded PHB magnetic nanoparticles in photooxidatizing simple biomolecules
Barbara Silva Figueiredo, Julyana Noval de Souza Ferreira, Vannyla Viktória Viana Vasconcelos, Joselito Nardy Ribeiro, Marco Cesar Cunegundes Guimarães, Arlan da Silva Gonçalves, and André Romero da Silva
Elsevier BV



Controlled biosynthesis of gold nanoparticles with Coffea arabica using factorial design
Wanderson Juvencio Keijok, Rayssa Helena Arruda Pereira, Luis Alberto Contreras Alvarez, Adilson Ribeiro Prado, André Romero da Silva, Josimar Ribeiro, Jairo Pinto de Oliveira, and Marco Cesar Cunegundes Guimarães
Springer Science and Business Media LLC
Abstract Green synthesis of metallic nanoparticles has become incredibly popular, mainly by minimizing problems of environmental contamination and by being able to reduce, stabilize and potentially functionalize nanomaterials. Such compounds have possible applications in various areas, e.g., pharmaceuticals (drug delivery systems, cosmetics), textile industry (clothing with antimicrobial properties), diagnostic medicine (imaging, high efficiency biosensors), energy (solar panels), bioremediation, among others. However, the lack of reproducibility and information on the control mechanisms during synthesis have made the application of green-synthesized nanoparticles unfeasible. Thus, this study proposed the investigation of the main mechanisms affecting synthesis control, using factorial design for the preparation of gold nanoparticles with extract of Coffea arabica. We obtained stable (Zeta Potential, UV-vis and DLS), monodisperse, and quasi-spherical (TEM) nanoparticles, which presented adsorbed aromatic molecules (FTIR and RAMAN) and defined crystal structure (XRD), proving that the plant extract acted as a reducing agent, as well as a stabilizer and functionalizer for the synthesized nanostructures. The factorial design employed here to obtain gold nanoparticles with Coffea arabica extract allowed for a controlled and reproducible synthesis, enabling new possibilities for the application in several fields.


Antioxidant, antimicrobial and cytotoxic activities of gold nanoparticles capped with quercetin
Felipe Guzansky Milanezi, Leandra Martins Meireles, Marcella Malavazi de Christo Scherer, Jairo P. de Oliveira, André Romero da Silva, Mariceli Lamas de Araujo, Denise Coutinho Endringer, Marcio Fronza, Marco Cesar Cunegundes Guimarães, and Rodrigo Scherer
Elsevier BV
In the present work, we report the antioxidant, antimicrobial and cytotoxic activities of quercetin-capped gold nanoparticles (AuNPsQct). The synthesis of AuNPsQct was confirmed by UV–Vis spectroscopy, FTIR and transmission electron microscopy (TEM) analyses. The FTIR spectrum showed the integrity of the quercetin molecules on the nanoparticle surface. The TEM images showed sizes less than 100 nm and a slight spherical shape. The electrostatic stability was confirmed by the zeta potential method. The antioxidant activity of quercetin, evaluated by DPPH, ABTS and nitric oxide free radical scavenging methods, was preserved in the gold nanoparticles, furthermore quercetin-capped gold nanoparticles (IR50 0.37 µg/mL) demonstrated a higher antioxidant activity than free quercetin (IR50 0.57 µg/mL) by nitric oxide free radical scavenging method. Strong antifungal activity was observed for Aspergillus fumigatus with concentrations ranging from 0.1 to 0.5 mg/mL. The nanoparticles with quercetin did not exhibit cytotoxicity to human fibroblasts (L929 cells). In conclusion, these results suggest that AuNPsQct, produced by cost-effective method, can act as a promising candidate for different medical applications.


PLGA-PEG nanoparticles containing gallium phthalocyanine: Preparation, optimization and analysis of its photodynamic efficiency on red blood cell and Hepa-1C1C7
Drielly Lorenzoni, Carlos Augusto Zanoni Souto, Maiara Barboza Araujo, Carol de Souza Berger, Luciene Cristina Duarte da Silva, Mariana Ozello Baratti, Joselito Nardy Ribeiro, Denise Coutinho Endringer, Marco Cesar Cunegundes Guimarães, and André Romero da Silva
Elsevier BV
Poly(lactide-co-glycolide) (PLGA) has been used for the encapsulation of phthalocyanine motived by its biocompatibility and biodegradability. Many studies have already been done to evaluate the influence of parameters used in the PLGA nanoparticle synthesis but without the evaluation of the combinatory interaction between these parameters on the nanoparticulate properties. Ga(III)-phthalocyanine (GaPc) was encapsulated into the PEGlated PLGA-nanoparticles and the individual and combinatory effects of the emulsification time, the method used for the nanoparticle synthesis and the temperature of the aqueous phase was evaluated on the size, entrapment efficiency, efficacy of nanoparticle recovery, residual PVA and zeta potential value using a 23 factorial design (FD). Mathematical models were adjustable to the data and evolutionary operations were performed to optimize the nanoparticle size. The ability of the optimized nanoparticle to decrease the viability of the Hepa-1C1C7 cell and the blood red cell was also evaluated. The FD disclosed the emulsification-diffusion method decreased the residual PVA and the size of PLGA-PEG nanoparticle, but also decreased the entrapment efficiency of GaPc, the zeta potential absolute value and the recovery efficacy of nanoparticles. The combinatory effect between the method used in the nanoparticle preparation and the temperature of aqueous phase influenced four of the five evaluated properties. The viability of Hepa-1C1C7 cells was reduced until 13× when the cells were irradiated in the presence of encapsulated GaPc while it was decreased until 4.7× when the experiment was carried out with the free GaPc. The encapsulated GaPc was also more efficient to cause the haemolysis of the RBC than it was the free GaPc. The optimization of the nanoparticles synthesis increased the efficiency of the GaPc to oxidize the evaluated cells.


Virola oleifera-capped gold nanoparticles showing radical-scavenging activity and low cytotoxicity
Andressa dos Santos Corrêa, Luis Alberto Contreras, Wanderson Juvencio Keijok, Divan Henrique Fernandes Barcelos, Ana Cláudia Hertel Pereira, Rodrigo Rezende Kitagawa, Rodrigo Scherer, Daniel Cláudio de Oliveira Gomes, André Romero da Silva, Denise Coutinho Endringer,et al.
Elsevier BV
The development of effective nanoparticle therapeutics has been hindered by their surface characteristics, such as hydrophobicity and charge. Therefore, the success of biomedical applications with nanoparticles is governed by the control of these characteristics. In this article, we report an efficient green capping method for gold nanoparticles (AuNPs) by a reduction with sodium citrate and capping with Virola oleifera (Vo), which is a green exudate rich in polyphenols and flavonoids. The Vo-capped AuNPs were characterized by UV, DLS, FTIR, Raman, TEM, DPPH, FRAP and their cytotoxicity was evaluated on the viability of Murine macrophage cell. The AuNPs had an average particle size of 15 nm and were stable over a long time, as indicated by their unchanged SPR and zeta potential values. These nanoparticles were assessed for their antioxidant potential using DPPH and FRAP and demonstrated the highest antioxidant activities and low cytotoxicity. We propose that the Virola oleifera-capped AuNPs have potential biomedical applications.


Extracellular biosynthesis of silver nanoparticles using the cell-free filtrate of nematophagous fungus Duddingtonia flagrans
Laryssa Pinheiro Costa Silva, Jairo Pinto Oliveira, Wanderson Juvencio Keijok, André Romero da Silva, Anderson Rocha Aguiar, Marco Cesar Cunegundes Guimarães, Carolina Magri Ferraz, Jackson Victor Araújo, Fernando Luiz Tobias, and Fabio Ribeiro Braga
Informa UK Limited
The biosynthesis of metallic nanoparticles (NPs) using biological systems such as fungi has evolved to become an important area of nanobiotechnology. Herein, we report for the first time the extracellular synthesis of highly stable silver NPs (AgNPs) using the nematophagous fungus Duddingtonia flagrans (AC001). The fungal cell-free filtrate was analyzed by the Bradford method and 3,5-dinitrosalicylic acid assay and used to synthesize the AgNPs in the presence of a 1 mM AgNO3 solution. They have been characterized by UV–Vis spectroscopy, X-ray diffraction, transmission electron microscopy, dynamic light scattering, Zeta potential measurements, Fourier-transform infrared, and Raman spectroscopes. UV–Vis spectroscopy confirmed bioreduction, while X-ray diffractometry established the crystalline nature of the AgNPs. Dynamic light scattering and transmission electron microscopy images showed approximately 11, 38 nm monodisperse and quasispherical AgNPs. Zeta potential analysis was able to show a considerable stability of AgNPs. The N–H stretches in Fourier-transform infrared spectroscopy indicate the presence of protein molecules. The Raman bands suggest that chitinase was involved in the growth and stabilization of AgNPs, through the coating of the particles. Our results show that the NPs we synthesized have good stability, high yield, and monodispersion.


Determination of structural and thermodynamic parameters of bovine α-trypsin isoform in aqueous-organic media
Dayanne Pinho Rosa, Evaldo Vitor Pereira, Antonio Victor Baioco Vasconcelos, Maria Aparecida Cicilini, André Romero da Silva, Caroline Dutra Lacerda, Jamil Silvano de Oliveira, Marcelo Matos Santoro, Juliana Barbosa Coitinho, and Alexandre Martins Costa Santos
Elsevier BV
The α-trypsin isoform is a globular protein that belongs to serine-protease family and has a polypeptide chain of 223 amino acid residues, six disulfide bridges and two domains with similar structures. The effects of aqueous-organic solvent (ethanol) in different concentration on the α-trypsin structure have been investigated by spectroscopic techniques and thermodynamic data analysis. The results from spectroscopic measurements, including far-UV Circular Dichroism, UV-vis absorption spectroscopy, intrinsic tryptophan fluorescence and dynamic light scattering (DLS) suggest the formation of partially folded states, instead of aggregate states, at high ethanol concentration (>60% v/v ethanol), with little loss of secondary structure, but with significant tertiary structure changes. The thermodynamic data (Tm and ΔH) suggest a loosening of intramolecular weak interactions, which reflects in a flexibility increase such that the catalytic capacity can be increased or decreased according to the ethanol concentration into the system. Overall results we suggest that in range of 0-60% v/v ethanol/buffer, α-trypsin undergoes reversible multimerization phenomena with catalytic activity. However from 60% v/v ethanol/buffer, population of folded partially states with less catalytic activity are predominant.


The photobleaching of the free and encapsulated metallic phthalocyanine and its effect on the photooxidation of simple molecules
Brenda Gomes Fanchiotti, Marcella Piffer Zamprogno Machado, Letícia Camilato de Paula, Mahmut Durmuş, Tebello Nyokong, Arlan da Silva Gonçalves, and André Romero da Silva
Elsevier BV
The photobleaching of an unsubstituted phthalocyanine (gallium(III) phthalocyanine chloride (GaPc)) and a substituted phthalocyanine (1,4-(tetrakis[4-(benzyloxy)phenoxy]phthalocyaninato) indium(III) chloride (InTBPPc)) was monitored for the free photosensitizers and for the phthalocyanines encapsulated into nanoparticles of PEGylated poly(D,L-lactide-co-glycolide) (PLGA-PEG). Phosphate-buffered solutions (PBS) and organic solutions of the free GaPc or the free InTBPPc, and suspensions of each encapsulated photosensitizer (2-15μmol/L) were irradiated using a laser diode of 665nm with a power of 1-104mW and a light dose of 7.5J/cm2. The relative absorbance (RA) of the free GaPc dissolved in 1-methyl-2-pyrrolidone (MP) decreased 8.4 times when the laser power increased from 1mW to 104mW. However, the free or encapsulated GaPc did not suffer the photobleaching in PBS solution. The RA values decreased 2.4 times and 22.2 times for the free InTBPPc dissolved in PBS solution and in dimethylformamide (DMF), respectively, but the encapsulated InTBPPc was only photobleached when the laser power was 104mW at 8μmol/L. The increase of the free GaPc concentration favored the photobleaching in MP until 8μmol/L while the increase from 2μmol/L to 5μmol/L reduced the photodegradation in PBS solution. However, the photobleaching of the free InTBPPc in DMF or in PBS solution, and of each encapsulated photosensitizer was not influenced by increasing the concentration. The influence of the photobleaching on the capability of the free and encapsulated GaPc and InTBPPc to photooxidate the simple molecules was investigated monitoring the fluorescence of dimethylanthracene (DMA) and the tryptophan (Trp). Free InTBPPc was 2.0 and 1.8 times faster to photooxidate the DMA and Trp than it was the free GaPc, but the encapsulated GaPc was 3.4 times more efficient to photooxidize the Trp than it was the encapsulated InTBPPc due to the photodegradation suffered by the encapsulated InTBPPc. The participation of the singlet oxygen was confirmed with the sodium azide in the photobleaching of all free and encapsulated photosensitizer, and in the photooxidation of the DMA and Trp. The asymmetry of InTBPPc increased the solubility of the free compound, decreasing the aggregation state of the photosensitizer and favoring the photobleaching process. The encapsulation shows capability in decreasing the photobleaching of both photosensitizers but the confocal micrographs showed that the increase of the solubility favored the InTBPPc photobleaching during the acquisition of optical cross section.


Facile Synthesis of Monodisperse Gold Nanocrystals Using Virola oleifera
Bárbara A. Milaneze, Jairo P. Oliveira, Ingrid Augusto, Wanderson J. Keijok, Andressa S. Côrrea, Débora M. Ferreira, Otalíbio C. Nunes, Rita de Cássia R. Gonçalves, Rodrigo R. Kitagawa, Vinícius G. Celante,et al.
Springer Science and Business Media LLC
AbstractThe development of new routes and strategies for nanotechnology applications that only employ green synthesis has inspired investigators to devise natural systems. Among these systems, the synthesis of gold nanoparticles using plant extracts has been actively developed as an alternative, efficient, cost-effective, and environmentally safe method for producing nanoparticles, and this approach is also suitable for large-scale synthesis. This study reports reproducible and completely natural gold nanocrystals that were synthesized using Virola oleifera extract. V. oleifera resin is rich in epicatechin, ferulic acid, gallic acid, and flavonoids (i.e., quercetin and eriodictyol). These gold nanoparticles play three roles. First, these nanoparticles exhibit remarkable stability based on their zeta potential. Second, these nanoparticles are functionalized with flavonoids, and third, an efficient, economical, and environmentally friendly mechanism can be employed to produce green nanoparticles with organic compounds on the surface. Our model is capable of reducing the resin of V. oleifera, which creates stability and opens a new avenue for biological applications. This method does not require painstaking conditions or hazardous agents and is a rapid, efficient, and green approach for the fabrication of monodisperse gold nanoparticles. Graphical AbstractThe Virola oleifera reduction method for the synthesis of gold nanoparticles (AuNP’s)


Effects of preparation conditions of poly(lactide-co-glycolide) nanoparticles loaded with amphiphilic porphyrins and their photoactivities
Juliana Machado da Silveira, André Romero da Silva, Mathias O. Senge, and Renato Atilio Jorge
American Scientific Publishers
Three porphyrins, (5,10,15,20-tetra(3-hydroxyphenyl)porphyrin, 5-hexyl-10,20-bis(3-hydroxyphenyl)-porphyrin and 5-hexyl-10,15,20-tris(3-hydroxyphenyl)porphyrin), with different amphiphilicities and equal singlet oxygen quantum yields in ethanol, were encapsulated into 50:50 poly(lactide-co-glycolide), nanoparticles prepared by the emulsion/evaporation technique. A 22 factorial design was utilized to evaluate the influence of the porphyrin/polymer mass ratio and the percentage of ethanol in the aqueous phase on the size and zeta potential of the nanoparticles. Increasing both the amount of ethanol and the porphyrin/polymer ratio decreases the size and increases zeta potential for the photosensitizers studied, except for 5-hexyl-10,15,20-tri(3-hydroxyphenyl)porphyrin. Entrapment efficiency depended on the individual m-hydroxyphenylporphyrin and ranged from 69 to 97%. After 1.5 h incubation with m-hydroxyphenylporphyrin-loaded nanoparticles the percentages of intracellular uptake were the same for all porphyrins since the molecules are confined in the nanoparticles, hampering the interaction of the amphiphilic photosensitizers with the cellular membrane. All encapsulated porphyrins caused the same decrease of cell viability and always localized in the perinuclear region of the cells. Results show that these m-hydroxyphenylporphyrins, although with different amphiphilicities, have equal photodynamic efficacies.


Gamma trypsin: Purification and physicochemical characterization of a novel bovine trypsin isoform
Caroline Dutra Lacerda, Antônio Ernani. Teixeira, Jamil Silvano de Oliveira, Silvana Fontoura Silva, Antônio Victor Baioco Vasconcelos, Débora Gonçalves Gouveia, André Romero da Silva, Marcelo Matos Santoro, Marcos Luiz dos Mares-Guia, and Alexandre Martins Costa Santos
Elsevier BV
A novel bovine trypsin isoform was purified from commercial sample by ion exchange chromatography by Sephadex SP C50®. New isoform contains in addition of loss of N-terminus hexapeptide (as found in parent molecule β-trypsin) an intra-chain split between Lys-155 and Ser-156. The novel enzyme denominate γ-trypsin showed similar properties with α-trypsin isoform in polypeptide number chain (two chain), molecular masses (23,312 Da), secondary structure, hydrodynamic radius and others. In spite of enzymatic and structural similarities of both isoforms, γ-trypsin preferably has a lower rate formation from β-trypsin, a lower surface charge, but the γ-trypsin has a higher thermal stability than α-trypsin. Due to obtaining facility of purification of bovine trypsin isoforms from commercial font, and properties described above, this enzyme becomes an interesting alternative for the food industry, detergent and biocatalysis research.


Improved photodynamic action of nanoparticles loaded with indium (III) phthalocyanine on MCF-7 breast cancer cells
Carlos Augusto Zanoni Souto, Klésia Pirola Madeira, Daniel Rettori, Mariana Ozello Baratti, Letícia Batista Azevedo Rangel, Daniel Razzo, and André Romero da Silva
Springer Science and Business Media LLC
Indium (III) phthalocyanine (InPc) was encapsulated into nanoparticles of PEGylated poly(d,l-lactide-co-glycolide) (PLGA-PEG) to improve the photobiological activity of the photosensitizer. The efficacy of nanoparticles loaded with InPc and their cellular uptake was investigated with MCF-7 breast tumor cells, and compared with the free InPc. The influence of photosensitizer (PS) concentration (1.8–7.5 μmol/L), incubation time (1–2 h), and laser power (10–100 mW) were studied on the photodynamic effect caused by the encapsulated and the free InPc. Nanoparticles with a size distribution ranging from 61 to 243 nm and with InPc entrapment efficiency of 72 ± 6 % were used in the experiments. Only the photodynamic effect of encapsulated InPc was dependent on PS concentration and laser power. The InPc-loaded nanoparticles were more efficient in reducing MCF-7 cell viability than the free PS. For a light dose of 7.5 J/cm2 and laser power of 100 mW, the effectiveness of encapsulated InPc to reduce the viability was 34 ± 3 % while for free InPc was 60 ± 7 %. Confocal microscopy showed that InPc-loaded nanoparticles, as well as free InPc, were found throughout the cytosol. However, the nanoparticle aggregates and the aggregates of free PS were found in the cell periphery and outside of the cell. The nanoparticles aggregates were generated due to the particles concentration used in the experiment because of the small loading of the InPc while the low solubility of InPc caused the formation of aggregates of free PS in the culture medium. The participation of singlet oxygen in the photocytotoxic effect of InPc-loaded nanoparticles was corroborated by electron paramagnetic resonance experiments, and the encapsulation of photosensitizers reduced the photobleaching of InPc.


Effects of preparation conditions on the characteristics of poly(lactide-co-glycolide) nanospheres loaded with chloro(5,10,15,20- tetraphenylporphyrinato)indium(III)
André Romero da Silva, Ana Maria de Oliveira, Fábio Augusto, and Renato Atilio Jorge
American Scientific Publishers
A 2(4-1) fractional factorial design was utilized to evaluate the influence of four preparation conditions on six characteristics of poly(lactide-co-glycolide) nanospheres loaded with chloro(5,10,15,20-tetraphenylporphyrinato)indium(III). Ethanol in the aqueous phase and the stirring rate were the factors that most influenced the nanosphere characteristics. An increase in these factors caused a decrease in nanosphere size, recovery yield and residual chloroform and an increase in the percent of residual poly(vinyl alcohol). The synergic interaction between these two factors caused an increase in the percent residual chloroform. The entrapment efficiency was increased by an increase of ethanol in the aqueous phase or an increase in the percent poly(vinyl alcohol), but an overall decrease was obtained due to a synergic interaction between these factors. The stirring rate was the only parameter that caused an increase of the zeta potential. Evolutionary operations were then carried out based on the results from the fractional factorial design and nanospheres were obtained with sizes smaller than 200 nm.


Drug release from microspheres and nanospheres of poly(lactide-co- glycolide) without sphere separation from the release medium




In vitro photodynamic activity of chloro(5,10,15,20-tetraphenylporphyrinato)indium(III) loaded-poly(lactide-co-glycolide) nanoparticles in LNCaP prostate tumour cells
André Romero da Silva, Natalia Mayumi Inada, Daniel Rettori, Mariana Ozello Baratti, Anibal Eugenio Vercesi, and Renato Atilio Jorge
Elsevier BV
In(III)-meso-tetraphenylporphyrin (InTPP) was encapsulated into nanoparticles (smaller than 200 nm) of poly(d,l-lactide-co-glycolide) (PLGA) using the emulsification-evaporation technique. The photodynamic efficacy of InTPP-loaded nanoparticles and its cellular uptake was investigated with LNCaP prostate tumour cells, in comparison with the free InTPP. The effects of incubation time (1-3h), drug concentration (1.8-7.7 micromol/L) and incident light dose (15-45 J/cm(2)) with both encapsulated and free InTPP were studied. The type of cell death induced by the photochemical process using both encapsulated and free InTPP was also investigated. Cell viability was reduced more significantly with increasing values of these effects for InTPP-loaded nanoparticles than with the free drug. The cellular death induced by both encapsulated and free InTPP was preponderantly apoptotic. Confocal laser scanning microscopy data showed that the InTPP-loaded nanoparticles, as well free InTPP, were localized in the cells, and always in the perinuclear region. Encapsulated InTPP was measured by the intensity of fluorescence intensity of cell extracts and was three times more internalized into the cells than was the free InTPP. Electron paramagnetic resonance experiments corroborated the participation of singlet oxygen in the photocytotoxic effect of nanoparticles loaded with InTPP.


Type II photooxidation mechanism of biomolecules using chloro(5,10,15,20- tetraphenylporphyrinato)indium(III) as a photosensitizer
André Romero da Silva, Joselito Nardy Ribeiro, Daniel Rettori, and Renato Atilio Jorge
FapUNIFESP (SciELO)
The photooxidation mechanism of bovine serum albumin (BSA), L-tryptophan (Trp) and red blood cells (RBC) by chloro(5,10,15,20-tetraphenylporphyrinato)indium(III) (InTPP) was investigated. The photooxidation rate of Trp, BSA and RBC by InTPP was decreased in the presence of NaN3. The presence of D2O increases the photooxidation rate of Trp and BSA and decreases that of RBC. This decrease is probably related to a reduction of the binding constant between InTPP and RBC in the presence of D2O. No significant change in biomolecule fluorescence or in the percent of hemolysis was observed when radical quenchers (ferricyanide, mannitol and dismutase superoxide) were used. Experiments using electron paramagnetic resonance (EPR) show that only 1O2 was generated by InTPP. A mechanistic model based on the preferential oxidation of Trp and BSA by singlet oxygen is proposed. The agreement between the experimental data and the kinetic model gives additional support to the predominance of a mechanism via 1O2 in biomolecule photooxidation by InTPP.


Photodynamic activity of chloro(5,10,15,20-tetraphenylporphyrinato) indium(III)
André R. da Silva, Alessandra C. Pelegrino, Antonio C. Tedesco, and Renato A. Jorge
FapUNIFESP (SciELO)
A atividade fotodinâmica do cloro(5,10,15,20-tetrafenilporfirinato) de indio(III) (InTPP) in vitro foi investigado para possivel uso em terapia fotodinâmica (PDT). O rendimento quântico de oxigenio singlete do InTPP (FD = 0,72) em DMSO foi maior que da 5,10,15,20-tetrafenilporfirina (TPP) (FD = 0,52). Os sitios de ligacao entre os fotossensibilizadores e albumina bovina (BSA) sao independentes e com celulas vermelhas de sangue humano (RBC) sao cooperativos, com um e quatro sitios de ligacao por molecula, respectivamente. As constantes de associacao com BSA sao (1,15 ± 0,07) × 105 e (2,6 ± 0,1) × 104 L mol-1 e com RBC sao (2,40 ± 0,05) × 107 L mol-1 e (7,2 ± 0,2) × 104 L mol-1 para InTPP e Photofrin®, respectivamente. O InTPP foi mais eficiente do que Photofrin® em fotooxidar L-triptofano (Trp) e BSA quando maiores concentracoes dos fotossensibilizadores foram utilizadas (acima de 14 µmol L-1). O InTPP foi 1,37 a 1,5 vezes mais eficaz em fotooxidar as RBC do que Photofrin®. Nossos resultados indicam que o InTPP pode ser usado para estudos futuros de PDT.


Irradiated cationic mesoporphyrin induces larger damage to isolated rat liver mitochondria than the anionic form
Natalia M. Inada, André R. da Silva, Renato A. Jorge, Jiri Borecký, and Anibal E. Vercesi
Elsevier BV
The action of irradiated cationic Fe(III)TMPyP and anionic Fe(III)TPPS4 forms of mesoporphyrins on mitochondrial functions was investigated using experimental conditions that caused minimal effects on mitochondria in the dark. Treatment of mitochondria with 1 microM Fe(III)TMPyP for 2 min decreased the respiratory control by 3% in the dark and 28% after irradiation. Fe(III)TPPS4 (1 microM) had no significant effect on respiratory control under any of the above conditions. Both porphyrins increased the mitochondrial production of reactive oxygen species in the presence of Ca2+; however, the effect of Fe(III)TMPyP was significantly stronger. In both cases, this overproduction was associated with membrane lipid peroxidation. It was also observed that the association constant of Fe(III)TMPyP with mitochondria was 11 times higher than that of Fe(III)TPPS4. In conclusion, the damage to isolated mitochondria induced by Fe(III)TMPyP under illumination was larger than by Fe(III)TPPS4, probably because its cationic charge favors association with the mitochondrial membrane. This is supported by the decrease in the association constant of Fe(III)TMPyP with mitochondria in higher salt medium.


Evaluation of photodynamic activity of porphyrins for use in photodynamic therapy through tryptophan photo-oxidation
J. N. Ribeiro, R. A. Jorge, A. R. da Silva, A. V. Flores, L. M. Ronchi, and A. C. Tedesco
FapUNIFESP (SciELO)
Neste trabalho investigou-se a atividade fotodinâmica de octaetilporfirina (OEP), octaetilporfirina de vanadil (VOOEP) e meso-tetramesitilporfirina (m-TMP). Este estudo foi realizado através da determinação da constante da velocidade de fotoxidação (k f) do aminoácido triptofano (Trp). A participação do oxigênio singlete nesta fotoxidação foi determinada através da adição de azida de sódio e água deuterada no meio de reação. Os valores de k f/10-4s-1 para a fotoxidação de Trp demonstraram que OEP (2,80 ± 0.05) é mais eficiente do que m-TMP (1,62 ± 0,07) e VOOEP (0,81 ± 0,08). Os valores de k f foram menores na presença de azida de sódio e maiores na presença de água deuterada, sugerindo que o oxigênio singlete é o responsável pela atividade fotodinâmica de OEP, VOOEP e m-TMP. Estes resultados sugerem também que as diferenças na atividade fotodinâmica entre as porfirinas podem ser associadas com as diferenças na estrutura molecular das mesmas. A presença do grupo vanadil (V=O) interfere claramente na atividade fotodinâmica de OEP causando considerável redução na sua eficiência.

Publications

Controlled biosynthesis of gold nanoparticles with Coffea arabica using factorial design
Scientific Reports
2019-12 | journal-article
DOI: 10.1038/s41598-019-52496-9Parte de ISSN: 2045-2322

PLGA-PEG nanoparticles containing gallium phthalocyanine: Preparation, optimization and analysis of its photodynamic efficiency on red blood cell and Hepa-1C1C7
Journal of Photochemistry and Photobiology B: Biology
2019-09 | journal-article
DOI: 10.1016/j. de ISSN: 1011-1344

Quantification of Inter-particle Spacing Caused by Thiol Self-Assembled Monolayers Using Transmission Electron Microscopy
Plasmonics
2019-06 | journal-article
DOI: 10.1007/s11468-018-0853-9Parte de ISSN: 1557-1955Parte de ISSN: 1557-1963

Antioxidant, antimicrobial and cytotoxic activities of gold nanoparticles capped with quercetin
Saudi Pharmaceutical Journal
2019 | journal-article
DOI: 10.1016/j. 2-

Virola oleifera-capped gold nanoparticles showing radical-scavenging activity and low cytotoxicity
Materials Science and Engineering: C
2018-10 | journal-article
DOI: 10.1016/j. de ISSN: 0928-4931

EVALUATION OF LIQUORICE GLYCYRRHIZA GLABRA L. ROOT POWDER AS A NEW ADSORBENT IN THE REMOVAL OF TEXTILE DYES IN AQUEOUS MEDIUM.
International Journal of Advanced Research
2018-08-31 | journal-article
DOI: 10.21474/ijar01/7520Parte de ISSN: 2320-5407

WOOD SAWDUST POWDER FROM CORYMBIA CITRIODORA TO CONGO RED TOXIC DYE ADSORPTION
INDIAN JOURNAL OF APPLIED RESEARCH
2018-07 | journal-article
DOI: 10.36106/ijar

Determination of structural and thermodynamic parameters of bovine α-trypsin isoform in aqueous-organic media
International Journal of Biological Macromolecules
2017-08 | journal-article
DOI: 10.1016/j. de ISSN: 0141-8130

Extracellular biosynthesis of silver nanoparticles using the cell-free filtrate of nematophagous fungus Duddingtonia flagrans
International Journal of Nanomedicine
2017-08 | journal-article
DOI: 10.2147/ de ISSN: 1178-2013

Facile Synthesis of Monodisperse Gold Nanocrystals Using Virola oleifera
Nanoscale Research Letters
2016-12 | journal-article
DOI: 10.1186/s11671-016-1683-3Parte de ISSN: 1931-7573Parte de ISSN: 1556-276X

The photobleaching of the free and encapsulated metallic phthalocyanine and its effect on the photooxidation of simple molecules
Journal of Photochemistry and Photobiology B: Biology
2016-12 | journal-article
DOI: 10.1016/j. de ISSN: 1011-1344

Banana Peel for Acetylsalicylic Acid Retention
Journal of Environmental Protection
2016 | journal-article
DOI: 10.4236/ de ISSN: 2152-2197Parte de ISSN: 2152-2219

Gamma trypsin: Purification and physicochemical characterization of a novel bovine trypsin isoform
International Journal of Biological Macromolecules
2014-09 | journal-article
DOI: 10.1016/j. de ISSN: 0141-8130

Effects of Preparation Conditions of Poly(lactide-co-glycolide) Nanoparticles Loaded with Amphiphilic Porphyrins and Their Photoactivities
Journal of Nanoscience and Nanotechnology
2014-08-01 | journal-article
DOI: 10.1166/ de ISSN: 1533-4880Parte de ISSN: 1533-4899

Improved photodynamic action of nanoparticles loaded with indium (III) phthalocyanine on MCF-7 breast cancer cells
Journal of Nanoparticle Research
2013-09 | journal-article
DOI: 10.1007/s11051-013-1879-8Parte de ISSN: 1388-0764Parte de ISSN: 1572-896X

Effects of Preparation Conditions on the Characteristics of Poly(lactide-co-glycolide) Nanospheres Loaded with Chloro(5,10,15,20-tetraphenylporphyrinato)indium(III)
Journal of Nanoscience and Nanotechnology
2011-06-01 | journal-article
DOI: 10.1166/ de ISSN: 1533-4880

Drug release from microspheres and nanospheres of poly(lactide-co- glycolide) without sphere separation from the release medium
Journal of the Brazilian Chemical Society
2010 | journal-article
EID: 2-

Avaliação pré-clínica de novas substâncias para uso como futuras drogas fotoquimioterápicas
ARQUIVOS CATARINENSES DE MEDICINA
2009 | journal-article

In vitro photodynamic activity of chloro(5,10,15,20-tetraphenylporphyrinato)indium(III) loaded-poly(lactide-co-glycolide) nanoparticles in LNCaP prostate tumour cells
Journal of Photochemistry and Photobiology B: Biology
2009 | journal-article
DOI: 10.1016/j. 2-

Photodynamic activity of chloro(5,10,15,20-tetraphenylporphyrinato) indium(III)
Journal of the Brazilian Chemical Society
2008 | journal-article
EID: 2-

Type II photooxidation mechanism of biomolecules using chloro(5,10,15,20- tetraphenylporphyrinato)indium(III) as a photosensitizer
Journal of the Brazilian Chemical Society
2008 | journal-article
EID: 2-

RESEARCH OUTPUTS (PATENTS, SOFTWARE, PUBLICATIONS, PRODUCTS)

2012/05
BR1020120105772
Pharmaceutical composition comprising nanospheres encapsulated indium phthalocyanine of a polymer and its preparation process with control of nanoparticulate properties

2008/10
PI08043787
Pharmaceutical Composition Comprising Nanospheres Encapsulated Indium Meso-Tetrafenylporphyrin (INTPP) and Process for Preparation thereof

2007/07
PI07030053
Determination process of release profile of encapsulated product

CONSULTANCY

Arbitration of scientific articles at Chemical Paper, Combinatorial Chemistry & High Throughput Screening, Current Nanoscience, Issues in Biological Sciences and Pharmaceutical Research, New Journal of Chemistry, Journal oh Nanoparticle Research, Journal of Nanoscience and Nanotechnology and Tetrahedron,

Industry, Institute, or Organisation Collaboration

Project: Urea granules coated with Ca and Mg nanoparticles as controlled release pathway for macro and micro nutrients
Industry: Granutec Tecnologia de Granulados
Support Source: Granutec Tecnologia de Granulados and Conselho Nacional de Desenvolvimento Científico e Tecnológico-CNPq
Financing Amount: BRL 76.126,56

INDUSTRY EXPERIENCE

2007/07 to 2008/08 - I worked at EMS Sigma Pharma S.A. as Supervisor Researcher of Project. In this time I work with drug delivery system.