Beatriz P. Nobre
@cqe.tecnico.ulisboa.pt
Centro de Quimica Estrutural
Centro de Quimica Estrutural
Beatriz P. Nobre, PhD in chemical Engineering, (Instituto Superior Técnico, Universidade de Lisboa, Portugal). Post-Doc at Bioenergy Unit of LNEG (Lisbon, Portugal) 2008-2014. Post-Doct at CQE (IST, Lisbon Portugal), IBB (IST, Universidade de Lisboa) and UCIBIO (NOVA School of Science and Technology) 2015-2018. Auxiliary Researcher at CQE (IST, Universidade de Lisboa), from 2018.
Her research interest concerned the use of green solvents, i.e. supercritical fluids, pressurized liquids in chemical engineering processes: extraction and fractionation processes of bioactive compounds from non-edible biomass and industrial waste residues; particle engineering for the production of micro and nanoparticles of bioactives, APIs, catalysts and encapsulates; catalytic heterogeneous reactions using green routes.
EDUCATION
PhD, Chemical Engineering, Lsbon University (Insituto Superior Técnico), Lisbon, Portugal
RESEARCH, TEACHING, or OTHER INTERESTS
Chemical Engineering, Process Chemistry and Technology, Chemistry, Biomaterials
Scopus Publications
Scopus Publications
Luís C. S. Nobre, Paula Teixeira, Carla I. C. Pinheiro, António M. F. Palavra, Mário J. F. Calvete, Carlos A. Nieto de Castro, and Beatriz P. Nobre
MDPI AG
The potential of the supercritical antisolvent micronization (SAS) technique was evaluated for the production of CaO-based particles with a size and a physical structure that could enable high performance for CO2 capture through the calcium looping process. Two sources of calcium derivative compounds were tested, waste marble powder (WMP) and dolomite. The SAS micronization of the derivate calcium acetate was carried out at 60 °C, 200 bar, a 0.5 mL min−1 flow rate of liquid solution, and 20 mg mL−1 concentration of solute, producing, with a yield of more than 70%, needle-like particles. Moreover, since dolomite presents with a mixture of calcium and magnesium carbonates, the influence of the magnesium fraction in the SAS micronization was also assessed. The micronized mixtures with lower magnesium content (higher calcium fraction) presented needle-like particles similar to WMP. On the other hand, for the higher magnesium fractions, the micronized material was similar to magnesium acetate micronization, presenting sphere-like particles. The use of the micronized material in the Ca-looping processes, considering 10 carbonation-calcination cycles under mild and realistic conditions, showed that under mild conditions, the micronized WMP improved CaO conversion. After 10 cycles the micronization, WMP presented a conversion 1.8 times greater than the unprocessed material. The micronized dolomite, under both mild and real conditions, maintained more stable conversion after 10 cycles.
T. Lopes da Silva, J. Vries, A. Reis, and B.P. Nobre
CRC Press
Nuno Reis Conceição, Beatriz P. Nobre, Atash V. Gurbanov, António M. F. Palavra, M. Fátima C. Guedes da Silva, Kamran T. Mahmudov, and Armando J. L. Pombeiro
MDPI AG
Two tetranuclear and two mononuclear Cu(II) complexes with arylhydrazones of malononitrile derived ligands (compounds 1–2 and 3–4, respectively), one trinuclear Co(II/III) complex with an arylhydrazone of acetoacetanilide (5) and one tetranuclear Zn(II) complex of 3-(2-carboxyphenyl-hydrazone)pentane-2,4-dione (6) were screened as potential catalysts in the peroxidative oxidation of cyclohexane by aqueous H2O2 in acetonitrile. The best results were attained in the presence of pyrazine-2-carboxylic acid (PCA) with 1 (26% yield, TON = 52.0) and with 2 (24%, TON = 48.0) after 4 h at 40 °C. In the presence of complexes 5 and 6, no oxygenated products were detected in the studied conditions. The employment of non-conventional conditions like supercritical carbon dioxide (scCO2) as reaction medium or microwave (MW) irradiation was assessed for complexes 1 and 2. After 6 h in acetonitrile–scCO2, at 50 °C and with HNO3 as promoter, only 17% yield was achieved using 1 as catalyst, and 21% using 2. Total yields of oxygenates up to 14 (with 1) and 13% (2) and TOFs of 56.0 and 52.0 h−1, respectively, were obtained working under MW irradiation at 70 °C and for the much shorter time of 0.5 h.
Carla Dias, Beatriz P. Nobre, José A.L. Santos, Teresa Lopes da Silva, and Alberto Reis
Elsevier BV
Carla Dias, Beatriz Nobre, José A. L. Santos, Alberto Reis, and Teresa Lopes da Silva
Springer Science and Business Media LLC
Nuno Reis Conceição, Beatriz P. Nobre, Anirban Karmakar, António M.F. Palavra, Kamran T. Mahmudov, M. Fátima C. Guedes da Silva, and Armando J.L. Pombeiro
Elsevier BV
Clarinda Costa, Beatriz Nobre, Ana Sofia Matos, Ana Sofia Silva, Teresa Casimiro, Maria Luísa Corvo, and Ana Aguiar-Ricardo
Elsevier BV
José Coelho, Paolo Trucillo, Beatriz Nobre, António Figueiredo Palavra, Roberta Campardelli, and Ernesto Reverchon
De Gruyter
José Coelho, Paolo Trucillo, Beatriz Nobre, António Figueiredo Palavra, Roberta Campardelli, and Ernesto Reverchon
Walter de Gruyter GmbH
Abstract Supercritical fluid (SCF) technologies have emerged as a real alternative to various natural product extraction processes and pharmaceutical production to obtain micronized particles, coprecipitates, nanocomposite polymer structures and liposomes, in addition to other increasingly larger applications described in literature. In the present work, a brief literature review of the application of supercritical fluid extraction (SFE) is presented. This is evidenced by several publications and patents, contributions from several countries and the increase of industries around the world dedicated to this technique. Next, we aim to focus the analysis of SFE on a review of the literature applied to microalgae as a substitute primitive feedstock due to its high growth rate, valuable biologically active lipophilic substances, and photosynthetic efficiency without competition with food sources or needs of arable lands. We finally discussing an SCF bioprocess with a very new perspective for liposome production focalized on its potential at industrial scale.
Luis C.S. Nobre, Samuel Santos, António M.F. Palavra, Mário J.F. Calvete, Carlos A. Nieto de Castro, and Beatriz P. Nobre
Elsevier BV
José Coelho, Jerson Veiga, Amin Karmali, Marisa Nicolai, Catarina Pinto Reis, Beatriz Nobre, and António Palavra
MDPI AG
Interest in new products from aromatic plants as medical and nutritional compounds is increasing. The aim of this work was to apply different extraction methods, including the use of supercritical carbon dioxide extraction, and to test the antioxidant activity of basil (Ocimum basilicum L.) extracts. In vitro efficacy assessments were performed using enzymatic assays. Essential oil obtained by hydrodistillation and volatile oil obtained from supercritical fluid extraction were analyzed by gas chromatography to quantify components. The total phenolic content in the extracts ranged from 35.5 ± 2.9 to 85.3 ± 8.6 mg of gallic acid equivalents and the total flavonoid content ranged from 35.5 ± 2.9 to 93.3 ± 3.9 micromole catechin equivalents per gram of dry weight of extract. All the extracts showed an antioxidant activity with 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid (ABTS), and the reducing power test. Extracts obtained from methanol had a higher antioxidant capacity per the DPPH test results (IC50 = 3.05 ± 0.36 mg/mL) and the reducing power test assay 306.8 ± 21.8 μmol of trolox equivalents per gram of extract (TE/g) compared with ethanolic or supercritical fluid extracts. However, using the ABTS assay, the extract obtained by supercritical fluid extraction had a higher antioxidant capacity with an IC50 of 1.74 ± 0.05 mg/mL. Finally, the examined extracts showed practically no acetylcholinesterase (AChE) inhibitory capacity and a slight inhibitory activity against tyrosinase.
S.P.M Ventura, B.P. Nobre, F. Ertekin, M. Hayes, M. Garciá-Vaquero, F. Vieira, M. Koc, L. Gouveia, M.R. Aires-Barros, and A.M.F. Palavra
Elsevier
Helena Sovová, Beatriz Nobre, and António Palavra
MDPI AG
Microalgae contain valuable biologically active lipophilic substances such as omega-3 fatty acids and carotenoids. In contrast to the recovery of vegetable oils from seeds, where the extraction with supercritical CO2 is used as a mild and selective method, economically viable application of this method on similarly soluble oils from microalgae requires, in most cases, much higher pressure. This paper presents and verifies hypothesis that this difference is caused by high adsorption capacity of microalgae. Under the pressures usually applied in supercritical fluid extraction from plants, microalgae bind a large fraction of the extracted oil, while under extremely high CO2 pressures their adsorption capacity diminishes and the extraction rate depends on oil solubility in supercritical CO2. A mathematical model for the extraction from microalgae was derived and applied to literature data on the extraction kinetics in order to determine model parameters.
R. Pacheco, A.F. Ferreira, T. Pinto, B.P. Nobre, D. Loureiro, P. Moura, L. Gouveia, and C.M. Silva
Elsevier BV
Blanca Araya, Luísa Gouveia, Beatriz Nobre, Alberto Reis, Rolando Chamy, and Paola Poirrier
Elsevier BV
C.A. Santos, B. Nobre, T. Lopes da Silva, H.M. Pinheiro, and A. Reis
Elsevier BV
Cláudia Freitas, Beatriz Nobre, Luísa Gouveia, José Roseiro, Alberto Reis, and Teresa Lopes da Silva
Elsevier BV
Luísa Gouveia, Carole Neves, Diogo Sebastião, Beatriz P. Nobre, and Cristina T. Matos
Elsevier BV
Raffaela Cutzu, Ana Clemente, Alberto Reis, Beatriz Nobre, Ilaria Mannazzu, José Roseiro, and Teresa Lopes da Silva
Oxford University Press (OUP)
Abstract Flow cytometry was used to assess β-carotene content, cell membrane permeability, cell size and granularity in Rhodotorula glutinis mutant 400A15 grown under different oxygen transfer coefficients (k L a) and carbon to nitrogen ratios (C/N). A Doehlert distribution was used in order to select the best conditions that induced the highest carotenoids production. The highest β-carotene content (0.79 mg g−1 DCW) at the lowest k L a and C/N (5 × 10−3 s−1 and 11.3 respectively). Under these conditions, the biomass concentration attained 18.60 g L−1. The highest ratio of cells with permeabilised membranes (2.6 %), and the highest cell size and granularity were also obtained under these conditions. It was observed that C/N showed a stronger influence than the k L a on the measured cell parameters.
L. Campenni’, B. P. Nobre, C. A. Santos, A. C. Oliveira, M. R. Aires-Barros, A. M. F. Palavra, and L. Gouveia
Springer Science and Business Media LLC
Ana F. Ferreira, Lauro A. Ribeiro, Ana P. Batista, Paula A.S.S. Marques, Beatriz P. Nobre, António M.F. Palavra, Patrícia Pereira da Silva, Luísa Gouveia, and Carla Silva
Elsevier BV
B.P. Nobre, F. Villalobos, B.E. Barragán, A.C. Oliveira, A.P. Batista, P.A.S.S. Marques, R.L. Mendes, H. Sovová, A.F. Palavra, and L. Gouveia
Elsevier BV
C.T. Matos, M. Santos, B.P. Nobre, and L. Gouveia
Elsevier BV
Jose P. Coelho, Ana F. Cristino, Patrícia G. Matos, Amélia P. Rauter, Beatriz P. Nobre, Rui L. Mendes, João G. Barroso, Ana Mainar, Jose S. Urieta, João M. N. A. Fareleira,et al.
MDPI AG
An overview of the studies carried out in our laboratories on supercritical fluid extraction (SFE) of volatile oils from seven aromatic plants: pennyroyal (Mentha pulegium L.), fennel seeds (Foeniculum vulgare Mill.), coriander (Coriandrum sativum L.), savory (Satureja fruticosa Béguinot), winter savory (Satureja montana L.), cotton lavender (Santolina chamaecyparisus) and thyme (Thymus vulgaris), is presented. A flow apparatus with a 1 L extractor and two 0.27 L separators was built to perform studies at temperatures ranging from 298 to 353 K and pressures up to 30.0 MPa. The best compromise between yield and composition compared with hydrodistillation (HD) was achieved selecting the optimum experimental conditions of extraction and fractionation. The major differences between HD and SFE oils is the presence of a small percentage of cuticular waxes and the relative amount of thymoquinone, an oxygenated monoterpene with important biological properties, which is present in the oils from thyme and winter savory. On the other hand, the modeling of our data on supercritical extraction of volatile oil from pennyroyal is discussed using Sovová’s models. These models have been applied successfully to the other volatile oil extractions. Furthermore, other experimental studies involving supercritical CO2 carried out in our laboratories are also mentioned.
Beatriz P. Nobre, Luisa Gouveia, Patricia G. S. Matos, Ana F. Cristino, António F. Palavra, and Rui L. Mendes
MDPI AG
Supercritical fluid extraction of all-E-lycopene from tomato industrial wastes (mixture of skins and seeds) was carried out in a semi-continuous flow apparatus using ethane as supercritical solvent. The effect of pressure, temperature, feed particle size, solvent superficial velocity and matrix initial composition was evaluated. Moreover, the yield of the extraction was compared with that obtained with other supercritical solvents (supercritical CO2 and a near critical mixture of ethane and propane). The recovery of all-E-lycopene increased with pressure, decreased with the increase of the particle size in the initial stages of the extraction and was not practically affected by the solvent superficial velocity. The effect of the temperature was more complex. When the temperature increased from 40 to 60 °C the recovery of all-E-lycopene increased from 80 to 90%. However, for a further increase to 80 °C, the recovery remained almost the same, indicating that some E-Z isomerization could have occurred, as well as some degradation of lycopene. The recovery of all-E-lycopene was almost the same for feed samples with different all-E-lycopene content. Furthermore, when a batch with a higher all-E-lycopene content was used, supercritical ethane and a near critical mixture of ethane and propane showed to be better solvents than supercritical CO2 leading to a faster extraction with a higher recovery of the carotenoid.