laura colombo

Scopus Publications

Longitudinal and combined assessment of 24(S)-hydroxycholesterol and Neurofilament light chain in the early stages of Huntington's disease
Lidia Sarro, Marta Valenza, Alessia Mongelli, Alice Passoni, Anna Castaldo, et al.
Neurobiology of Disease, 2026
Corrigendum to ‘Insights into kinetics, release, and behavioral effects of brain-targeted hybrid nanoparticles for cholesterol delivery in Huntington's disease’ J Control Release. 2021 Feb 10;330:587–598 (Journal of Controlled Release (2021) 330 (587–598), (S0168365920307756), (10.1016/j.jconrel.2020.12.051))
Giulia Birolini, Marta Valenza, Ilaria Ottonelli, Alice Passoni, Monica Favagrossa, et al.
Journal of Controlled Release, 2025
Tau oligomers impair memory and synaptic plasticity through the cellular prion protein
C. Balducci, Franca Orsini, M. Cerović, M. Beeg, Beatrice Rocutto, et al.
Acta Neuropathologica Communications, 2025
Corrigendum to “Chronic cholesterol administration to the brain supports complete and long-lasting cognitive and motor amelioration in Huntington's disease” (Pharmacological Research (2023) 194, (S1043661823001792), (10.1016/j.phrs.2023.106823))
Giulia Birolini, Marta Valenza, Ilaria Ottonelli, Francesca Talpo, Lucia Minoli, et al.
Pharmacological Research, 2025
Xanthohumol destabilizes the structure of amyloid-β (Aβ) oligomers and promotes the formation of high-molecular-weight amorphous aggregates
Linda Molteni, Chiara Bruzzone, Diletta Ami, Ada De Luigi, Laura Colombo, et al.
International Journal of Biological Macromolecules, 2025
The AβA2V paradigm: From molecular insights to therapeutic strategies in Alzheimer's disease and primary tauopathies
Luisa Diomede, Andrea Conz, Michele Mosconi, Tatiana Stoilova, Matteo Paloni, et al.
Pharmacological Research, 2025
Thermal effects and biological response of breast and pancreatic cancer cells undergoing gold nanorod-assisted photothermal therapy
Leonardo Bianchi, Sara Baroni, Gabriela Paroni, Martina Bruna Violatto, Giulia Yuri Moscatiello, et al.
Journal of Photochemistry and Photobiology B Biology, 2024
A cutting-edge approach based on UHPLC-MS to simultaneously investigate oxysterols and cholesterol precursors in biological samples: Validation in Huntington's disease mouse model
Alice Passoni, Monica Favagrossa, Marta Valenza, Giulia Birolini, Alessia Lanno, et al.
Talanta Open, 2024
Genetic deletion of astrocytic calcineurin B1 prevents cognitive impairment and neuropathology development in acute and chronic mouse models of Alzheimer's disease
Laura Tapella, Giulia Dematteis, Pietro La Vitola, Susanna Leva, Elisa Tonelli, et al.
GLIA, 2024
Alzheimer's disease (AD) represents an urgent yet unmet challenge for modern society, calling for exploration of innovative targets and therapeutic approaches. Astrocytes, main homeostatic cells in the CNS, represent promising cell‐target. Our aim was to investigate if deletion of the regulatory CaNB1 subunit of calcineurin in astrocytes could mitigate AD‐related memory deficits, neuropathology, and neuroinflammation. We have generated two, acute and chronic, AD mouse models with astrocytic CaNB1 ablation (ACN‐KO). In the former, we evaluated the ability of β‐amyloid oligomers (AβOs) to impair memory and activate glial cells once injected in the cerebral ventricle of conditional ACN‐KO mice. Next, we generated a tamoxifen‐inducible astrocyte‐specific CaNB1 knock‐out in 3xTg‐AD mice (indACNKO‐AD). CaNB1 was deleted, by tamoxifen injection, in 11.7‐month‐old 3xTg‐AD mice for 4.4 months. Spatial memory was evaluated using the Barnes maze; β‐amyloid plaques burden, neurofibrillary tangle deposition, reactive gliosis, and neuroinflammation were also assessed. The acute model showed that ICV injected AβOs in 2‐month‐old wild type mice impaired recognition memory and fostered a pro‐inflammatory microglia phenotype, whereas in ACN‐KO mice, AβOs were inactive. In indACNKO‐AD mice, 4.4 months after CaNB1 depletion, we found preservation of spatial memory and cognitive flexibility, abolishment of amyloidosis, and reduction of neurofibrillary tangles, gliosis, and neuroinflammation. Our results suggest that ACN is crucial for the development of cognitive impairment, AD neuropathology, and neuroinflammation. Astrocyte‐specific CaNB1 deletion is beneficial for both the abolishment of AβO‐mediated detrimental effects and treatment of ongoing AD‐related pathology, hence representing an intriguing target for AD therapy.
Cell-Free and In Vivo Characterization of the Inhibitory Activity of Lavado Cocoa Flavanols on the Amyloid Protein Ataxin-3: Toward New Approaches against Spinocerebellar Ataxia Type 3
Barbara Sciandrone, Alessandro Palmioli, Carlotta Ciaramelli, Roberta Pensotti, Laura Colombo, et al.
ACS Chemical Neuroscience, 2024
Spinocerebellar ataxia type 3 (SCA3) is a neurodegenerative disorder characterized by ataxia and other neurological manifestations, with a poor prognosis and a lack of effective therapies. The amyloid aggregation of the ataxin-3 protein is a hallmark of SCA3 and one of the main biochemical events prompting its onset, making it a prominent target for the development of preventive and therapeutic interventions. Here, we tested the efficacy of an aqueous Lavado cocoa extract and its polyphenolic components against ataxin-3 aggregation and neurotoxicity. The combination of biochemical assays and atomic force microscopy morphological analysis provided clear evidence of cocoa flavanols' ability to hinder ATX3 amyloid aggregation through direct physical interaction, as assessed by NMR spectroscopy. The chemical identity of the flavanols was investigated by ultraperformance liquid chromatography-high-resolution mass spectrometry. The use of the preclinical model Caenorhabditis elegans allowed us to demonstrate cocoa flavanols' ability to ameliorate ataxic phenotypes in vivo. To the best of our knowledge, Lavado cocoa is the first natural source whose extract is able to directly interfere with ATX3 aggregation, leading to the formation of off-pathway species.
Characterization of the Laser-induced Thermal Outcome of Gold Nanorods-mediated Photothermal Therapy in Breast Cancer Cells
Leonardo Bianchi, Sara Baroni, Gabriela Paroni, Martina Bruna Violatto, Giulia Yuri Moscatiello, et al.
Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society EMBS, 2024
Chronic cholesterol administration to the brain supports complete and long-lasting cognitive and motor amelioration in Huntington's disease
Giulia Birolini, Marta Valenza, Ilaria Ottonelli, Francesca Talpo, Lucia Minoli, et al.
Pharmacological Research, 2023
Aβ1-6A2V(D) peptide, effective on Aβ aggregation, inhibits tau misfolding and protects the brain after traumatic brain injury
Luisa Diomede, Elisa R. Zanier, Federico Moro, Gloria Vegliante, Laura Colombo, et al.
Molecular Psychiatry, 2023
Transmission-selective muscle pathology induced by the active propagation of mutant huntingtin across the human neuromuscular synapse
Margarita C. Dinamarca, Laura Colombo, Urszula Brykczynska, Amandine Grimm, Isabelle Fruh, et al.
Frontiers in Molecular Neuroscience, 2023
A novel bio-inspired strategy to prevent amyloidogenesis and synaptic damage in Alzheimer’s disease
Marcella Catania, Laura Colombo, Stefano Sorrentino, Alfredo Cagnotto, Jacopo Lucchetti, et al.
Molecular Psychiatry, 2022
Dysmyelination and glycolipid interference caused by phenylalanine in phenylketonuria
Valeria Rondelli, Alexandros Koutsioubas, Emanuela Di Cola, Giovanna Fragneto, I. Grillo, et al.
International Journal of Biological Macromolecules, 2022
Alzheimer's Disease Prevention through Natural Compounds: Cell-Free, In Vitro, and In Vivo Dissection of Hop (Humulus lupulus L.) Multitarget Activity
Alessandro Palmioli, Valeria Mazzoni, Ada De Luigi, Chiara Bruzzone, Gessica Sala, et al.
ACS Chemical Neuroscience, 2022
Micro- and Nanoplastics’ Effects on Protein Folding and Amyloidosis
Joseph Windheim, Laura Colombo, Nora C. Battajni, Luca Russo, Alfredo Cagnotto, et al.
International Journal of Molecular Sciences, 2022
Synaptic and functional alterations in the development of mutant huntingtin expressing hiPSC‐derived neurons
Margarita C. Dinamarca, Laura Colombo, Natalia E. Tousiaki, Matthias Müller, Eline Pecho-Vrieseling
Frontiers in Molecular Biosciences, 2022
NMR-Driven Identification of Cinnamon Bud and Bark Components With Anti-Aβ Activity
Carlotta Ciaramelli, Alessandro Palmioli, Irene Angotti, Laura Colombo, Ada De Luigi, et al.
Frontiers in Chemistry, 2022
Biochemical and biophysical features of disease-associated tau mutants V363A and V363I
Ada De Luigi, Laura Colombo, Luca Russo, Caterina Ricci, Antonio Bastone, et al.
Biochimica Et Biophysica Acta Proteins and Proteomics, 2022
Microbiological-chemical sourced chondroitin sulfates protect neuroblastoma SH-SY5Y cells against oxidative stress and are suitable for hydrogel-based controlled release
Emiliano Bedini, Alfonso Iadonisi, Chiara Schiraldi, Laura Colombo, Diego Albani, et al.
Antioxidants, 2021
Sleep inhibition induced by amyloid-β oligomers is mediated by the cellular prion protein
F. Del Gallo, S. Bianchi, I. Bertani, Massimo Messa, L. Colombo, et al.
Journal of Sleep Research, 2021
C. elegans detects toxicity of traumatic brain injury generated tau
Elisa R. Zanier, Maria Monica Barzago, Gloria Vegliante, Margherita Romeo, Elena Restelli, et al.
Neurobiology of Disease, 2021
NMR-based Lavado cocoa chemical characterization and comparison with fermented cocoa varieties: Insights on cocoa's anti-amyloidogenic activity
Carlotta Ciaramelli, Alessandro Palmioli, Ada De Luigi, Laura Colombo, Gessica Sala, et al.
Food Chemistry, 2021