Pathology and Forensic Medicine, Research and Theory, Multidisciplinary
63
Scopus Publications
Scopus Publications
Wound Repair Using PVA–CA Electrospun Dressings and Cold Plasma In Vivo Jonalba M. Pereira, Emilia A. L. S. Arisawa, Antônio L. M. Maia‐Filho, José F. Silva, Nicoly Alves, Carolina H. da‐Silveira, Lucia Vieira Journal of Applied Polymer Science, 2026 Electrospun polymeric wound dressings that integrate biomimetic structure with bioactive stimulation represent an effective approach for accelerating tissue repair. This study evaluates electrospun poly(vinyl alcohol) nanofibers crosslinked with citric acid (PVA–CA), with or without silver nanoparticles (AgNPs), applied alone or in combination with non‐thermal plasma (NTP) for cutaneous wound healing in rats. The PVA–CA system provided a hydrophilic, porous scaffold with improved structural stability due to ester crosslinking. Thirty‐six male Wistar rats with full‐thickness dorsal skin wounds were distributed into six groups: Control, PVA–CA, PVA–CA + Ag, NTP, PVA–CA + NTP, and PVA–CA + Ag + NTP. Healing progression was evaluated macroscopically and histologically at 3, 7, and 14 days post‐injury. The PVA–CA + NTP group showed the highest healing efficacy, achieving a 96.6% wound area reduction at Day 14, versus 89.7% for the control. Histological analyses demonstrated accelerated re‐epithelialization, increased angiogenesis, and enhanced collagen deposition in this group, indicating advanced tissue remodeling. Although AgNP incorporation provided antimicrobial functionality, it did not further improve regeneration when combined with NTP. These results demonstrate that citric‐acid‐crosslinked electrospun PVA dressings synergistically potentiate the therapeutic effects of non‐thermal plasma, offering a promising platform for advanced wound healing applications.
Therapeutic Potential of Photobiomodulation in Early Recovery After Experimental Spinal Cord Injury in Rats: Histological and Biomechanical Analysis Débora Campos Chaves Correia, Leonardo Borges de Lima, Mário Oliveira Lima, Luis Filipe Karatanasov Beloni, Raduan Hage, Emilia Angela Lo Schiavo Arisawa Journal of Biophotonics, 2026 Spinal cord injury (SCI) leads to severe functional deficits, underscoring the critical need for new therapies. This study evaluated the efficacy of photobiomodulation (PBM) as an early, noninvasive treatment for induced SCI, using specific parameters (808 nm, 72 J/cm 2 , 100 mW). A total of 15 rats were divided into Control (C), SCI, and PBM groups. Efficacy was determined by an integrated approach, correlating ground reaction force (GRF) with quantitative histological assessment. The PBM group showed a significant reduction of secondary damage (33.3 ± 5.5 vs. 53.9 ± 7.0 in the SCI group; p = 0.0002) and preserved neural structure. This tissue preservation aligns with the GRF analysis, which demonstrated that the PBM group recovered gait patterns similar to the Control group. In conclusion, PBM effectively mitigates necrosis aerea, maintaining tissue integrity, improving functional recovery, and reinforcing the PBM's therapeutic potential as a promising translatable strategy for outcomes after SCI. Further research should include immunostaining of cells and larger samples.
Immunohistochemical Evaluation of Spinal Cord Injuries Treated with Amniotic Membrane Leonardo B. de Lima, Débora C. C. Correia, Luciana B. Sant’anna, Emilia A. L. S. Arisawa ACS Omega, 2026 High Resolution Image Download MS PowerPoint Slide Spinal cord injury (SCI) severely disrupts central nervous system (CNS) function by interrupting sensory and motor signal transmission, often resulting in permanent deficits due to the formation of a glial scar. Although the amniotic membrane (AM) is derived from the human placenta and is a promising biomaterial, its efficacy in treating SCI remains unexplored. This study investigates the therapeutic potential of AM fragments in a surgically induced acute SCI model in rats, focusing on preserving tissue integrity and modulating astrocyte distribution and reactivity. SCI was experimentally induced by a drop-weight mini-guillotine model in rats, which were subsequently allocated into three groups: Control (C), Injury (I), and Amniotic Membrane (AM), where a 4 cm 2 AM fragment was applied over the lesion. Animals were euthanized after 28 days for histological and immunohistochemical analysis of the T9-T10 region, specifically to assess Glial Fibrillary Acidic Protein (GFAP) expression and identify reactive astrocytes. The application of AM significantly preserved nervous tissue structure. The cystic cavity area in the AM group (9.00 ± 7.65) was drastically lower than in the Injury group (41.80 ± 11.30). Crucially, the AM fragments attenuated the progression of nervous tissue degeneration, limiting cavitation and glial scar formation while reducing astrocytic reactivity. These findings establish AM as a viable and effective scaffold for acute SCI treatment.
Bioabsorbable Poly(vinyl alcohol)–Citric Acid Dressings: Wound Healing Studies in an Experimental In Vivo Model Jonalba Mendes Pereira, Emilia Angela Lo Schiavo Arisawa, Antônio Luiz Martins Maia Filho, José Figueredo-Silva, Nicoly Alves, Carolina Hahn da Silveira, Lucia Vieira European Burn Journal, 2025 Background: The wound healing process presents notable challenges for nursing teams, requiring extensive knowledge of wound care materials. A nanoparticle-free, bioabsorbable pol-yvinyl alcohol (PVA) with citric acid (CA) dressing produced by simple electrospin-ning was evaluated to treat acute wound healing in rats. This PVA-CA combination promotes crosslinking, increases the dressing capacity of absorption and confers heal-ing properties due to the citric acid antioxidant action. Methods: The dressing was tested in a quantitative experiment on 1.9 cm acute dermatological lesions in rats (n = 12), com-paring the PVA-CA-treated group with the untreated control group (CG). Samples were collected at 3, 7 and 14 days after lesion induction to evaluate the inflammatory process and tissue healing. Results: The macroscopic and histological data on the third day showed similar characteristics in both groups; however, after fourteen days, the PVA-CA group exhibited complete healing, accompanied by recomposition of the skin layers, whereas the wounds in the CG did not close completely. Conclusions: The results highlight that electrospun PVA-AC dressings improve healing outcomes and constitute a prom-ising and affordable solution, providing a suitable environment for tissue repair, re-ducing inflammatory cell infiltration, blood vessel formation, and restoration of epi-thelial tissue, reducing the time of the healing process of acute wounds.
Saliva FTIR Spectra and Machine Learning for Autism Spectrum Disorder Diagnosis - Preliminary Study Mayara Moniz Vieira Pinto, Emilia Angela Lo Schiavo Arisawa, Leandro José Raniero, Tanmoy Bhattacharjee IEEE Photonics Journal, 2025 The diagnosis of Autism Spectrum Disorder (ASD) remains a challenge due to the lack of specific tests and biological markers. ASD is a neurodevelopmental disorder that affects individuals throughout their lives, and its diagnosis allows access to treatments that improve their prognosis. Saliva analysis by Fourier Transform Infrared Spectroscopy (FTIR), which was not previously reported, appears to be a promising diagnostic tool for ASD. This study acquired spectra from samples of 19 ASD and 19 control children. Spectral signatures suggest the dominance of protein secondary structures, β-pleated sheet and α-helix structures in ASD and control children, respectively. Support Vector Machine (SVM) gave the best diagnosis, with sensitivity, precision, and specificity being 92%, 94%, and 95%, respectively. Shapley values analysis to understand the impact of spectral features on the SVM classifier identified β-pleated and β-turn sheets as responsible for classification. Results indicate the potential of saliva-based FTIR for autism diagnosis, warranting a large-scale trial.
Histological analysis of Spinal Cord Injury treated with Amniotic Membrane Débora Campos Chaves Correia, Leonardo Borges de Lima, Luciana Barros Sant'Anna, Mario Oliveira Lima, Emilia Angela Lo Schiavo Arisawa Medicina Brazil, 2025 Spinal cord injury (SCI) is one of the most harmful syndromes that affects humans due to neuronal destruction and interruption of the nerve impulse transmission between axons. The conduction of motor, sensory, and autonomic responses below the level of the injury is seriously compromised, generating high treatment costs for the health system and a reduction in quality of life, stimulating research into new treatment protocols. This study aimed to evaluate the effectiveness of a biomaterial, the amniotic membrane (AM), to treat experimentally induced SCI. 15 adult rats were divided into three groups (n = 5): S (Sham), L (SCI without treatment), and AM (SCI treated with AM). Spinal cord injury was induced in the region T9-T10 by direct trauma, free-falling a weight (10 g, 2 mm flat edge) held on a mini guillotine, 25 mm above the exposed spinal cord. A fragment of AM, obtained from the human placenta after maternal consent, was applied to the injured area only in the AM group. After 28 days, specimens from the area of spinal cord injury were excised and subjected to routine histological procedures. Data from the semi-quantitative score, obtained from a scheme that assigned different scores to regions of the spinal cord, and from the quantitative analysis were subjected to parametric statistical analysis. Results showed that Group S presented medullary tissue without changes (score 0). In contrast, Group L presented numerous areas of cavitation in the dorsal and lateral regions of the white and gray matter (9.61 ± 6.60 p<0.001) with an intense inflammatory infiltrate. The AM group exhibited small areas of cavitation in the dorsal and lateral regions of the white matter and part of the dorsal columns in the gray matter (0.94 ± 1,03, p<0.001), with few inflammatory cells. The results suggest the effectiveness of AM in the treatment of induced SCI, characterized by a reduction in the evolution of inflammatory and degenerative processes in the central nervous tissue compared to the untreated group.
Development and validation of equipment for kinematic and sensory-motor analysis of experimental spinal injury induced in rats Luis Filipe Karaanasov Beloni, Alessandro Corrêa Mendes, Emília Ângela Lo Schiavo Arisawa, Fernanda Pupio Silva Lima, Luciana Barros Sant’Anna, Mário Oliveira Lima Medicina Brazil, 2025 Spinal cord injury (SCI) is defined as an injury to the spinal cord, a structure responsible for regulating numerous functions in the body. Depending on the level and degree of the SCI, a rapid and devastating loss of neurological functions of anatomical structures located below the level of the lesion is observed. The objective of this study was to develop and validate equipment for kinematic and sensorimotor analysis of rats with induced spinal cord injury. A glass box was manufactured in the shape of a linear corridor through which the animals moved. A transparent acrylic plate was positioned inside the box, fixed by nylon cables to a load cell. The system works as a suspended platform to capture the force vectors applied by the rats during their movement. The box was supported on two wooden supports on a table, and between the two supports below the glass box, a mirror was positioned at 45º in order to display the bottom of the glass box. A camera was positioned in front of the mirror 50cm away to capture images of the rats' paws with the aim of enabling measurements of the variables used in calculating the sciatic functional index. The data was exported and post-processed to obtain graphical, quantitative and comparative analysis of the results. To validate the equipment, a statistical measurement correlation model was used, and in addition, clinical trials were carried out verifying the average speed, load distribution force and the sciatic functional index of the rats. The equipment was capable of fully collecting the proposed data during the passage through the platform, as well as capturing images of the paws through the mirror and storing the information in logs for post-processing of the data. As a result of the interclass correlation coefficient (ICC), the equipment achieved indices of 0.967 for ground reaction force; 0.896 for the sciatic functional index (SFI) and 0.755 for average speed. The fact that there is, in single piece of equipment, a way to capture the ground reaction force digitally and in real time allowed the storage of this information in .log format files, which made it possible to process and interpret it in graphic form and tabulated, that is, a reliable and accurate way of representing the behavior of individuals while being evaluated. It is concluded that the equipment developed was capable of capturing and storing data relating to kinematic and sensorimotor parameters.
Photobiomodulation and amniotic membrane for treat tendon injury in rats MARIANA C. NICODEMO, EMILIA ANGELA L.S. ARISAWA, LUCIANA B. SANT’ANNA, RODRIGO LOPES-MARTINS Anais Da Academia Brasileira De Ciencias, 2024 Tendons, complex fibrous structures, are subjected to great tensions, which can give rise to the so-called tendinopathies. This study aimed to evaluate photobiomodulation and human Amniotic Membrane applied as single or combined therapies to treat induced Achilles tendon lesions. Seventy-five rats were divided into five groups (n=15): C- control Sham surgery; I- tendon injury; LA- tendon injury treated with photobiomodulation; AM- tendon injury treated with Amniotic Membrane; LAM- tendon injury + photobiomodulation and Amniotic Membrane, subdivided into three groups (n=5) with analysis at 3, 7, and 14 days. The tendon injuries were made with a 20 g weight released from a mini guillotine onto the ankle in dorsiflexion. AM and LAM groups received an Amniotic Membrane fragment while LA and LAM groups received transcutaneous photobiomodulation, using a 660 nm wavelength laser. The inflammatory cells showed statistical differences between groups C and I (p<0.05), I and AM (p<0.01), I and LA (p<0.05), and I and LAM (p<0.01). Both photobiomodulation and Amniotic Membrane were shown to enhance tendon repair, and the association of photobiomodulation plus Amniotic Membrane was the most effective treatment. We conclude that the association of photobiomodulation plus Amniotic Membrane was effective in accelerating and improving the tendon regeneration process.
Preclinical study of experimental burns treated with photobiomodulation and Human Amniotic Membrane, both isolated and associated Fernanda Cláudia Miranda Amorim, Emilia Ângela Loschiavo Arisawa, Luciana Barros Sant’anna, Ana Beatriz Mendes Rodrigues, Davidson Ribeiro Costa Revista Latino Americana De Enfermagem, 2023 Objective: to evaluate the effect of photobiomodulation with low-level 660 nm laser alone or associated with Human Amniotic Membrane in the repair of partial-thickness burns in rats. Method: an experimental study conducted with 48 male Wistar rats, randomized into four groups: Control, Human Amniotic Membrane, Low-Level Laser Therapy, and Low-Level Laser Therapy associated with Human Amniotic Membrane. The histopathological characteristics of the skin samples were analyzed 7 and 14 days after the burn. The data obtained were submitted to the Kolmogorov-Smirnov and Mann-Whitney tests. Results: the histological analysis of the burn injuries showed a decrease in inflammation (p<0.0001) and an increase in proliferation of fibroblasts (p<0.0001) mainly at 7 days in all treatments related to the control group. At 14 days, the greater effectiveness in accelerating the healing process was significant (p<0.0001) in the Low-Level Laser Therapy group associated with the Human Amniotic Membrane. Conclusion: the association of photobiomodulation therapies with the Human Amniotic Membrane allowed verifying a reduction in the healing process time of the experimental lesions, stimulating its proposal as a treatment protocol in partial-thickness burns.
Amniotic membrane as an option for treatment of acute achilles tendon injury in rats Mariana de Castro Nicodemo, Lia Renó das Neves, Josafá Carvalho Aguiar, Flaviane de Souza Brito, Isabelle Ferreira, Luciana Barros Sant'Anna, Leandro José Raniero, Rodrigo Álvaro Lopes Martins, Paulo Roxo Barja, Emilia Angela Lo Schiavo Arisawa Acta Cirurgica Brasileira, 2017
Low-level laser therapy and Calendula officinalis in repairing diabetic foot ulcers Ana Flávia Machado de Carvalho, Maura Cristina Porto Feitosa, Nayana Pinheiro Machado de Freitas Coelho, Veruska Cronemberger Nogueira Rebêlo, Juçara Gonçalves de Castro, Patrícia Regina Gomes de Sousa, Valrian Campos Feitosa, Emilia Angela Lo Schiavo Arisawa Revista Da Escola De Enfermagem, 2016
The low-level laser on acute myositis in rats Ana Flávia Machado de Carvalho, Paula Fernanda Batista de Sousa, Maura Cristina Porto Feitosa, Nayana Pinheiro Machado de Freitas Coelho, Esmeralda Maria Lustosa Barros, Valrian Campos Feitosa, Emília Angela Loschiavo Arisawa Acta Cirurgica Brasileira, 2015
Cenostigma macrophyllum Tul. on the healing of skin wounds in rats with Diabetes mellitus Nayana Pinheiro Machado de Freitas Coelho, Veruska Cronemberger Nogueira, Maria Angélica Gargione Cardoso, Luciano da Silva Lopes, Paulo Pedro do Nascimento, Evair dos Santos Rocha, Caio Leôncio Probo da Silva, Emília Ângela Loschiavo Arisawa Acta Cirurgica Brasileira, 2013
Spectral region optimization for raman-based optical biopsy of inflammatory lesions Luis Felipe das Chagas e Silva de Carvalho, Renata Andrade Bitar, Emília Ângela Loschiavo Arisawa, Adriana Aigotti Haberbeck Brandão, Kathia Maria Honório, Luiz Antônio Guimarães Cabral, Airton Abrahão Martin, Herculano da Silva Martinho, Janete Dias Almeida Photomedicine and Laser Surgery, 2010
Raman spectroscopy study of breast disease Marcelo Moreno, Leandro Raniero, Emília Ângelo Loschiavo Arisawa, Ana Maria do Espírito Santo, Edson Aparecido Pereira dos Santos, Renata Andrade Bitar, Airton Abrahão Martin Theoretical Chemistry Accounts, 2010
Thyroid tissue analysis through Raman spectroscopy Caroline S. B. Teixeira, Renata A. Bitar, Herculano S. Martinho, André B. O. Santos, Marco A. V. Kulcsar, Celso U. M. Friguglietti, Ricardo B. da Costa, Emilia Â. L. Arisawa, Airton A. Martin Analyst, 2009
Role of cervicitis in the Raman-based optical diagnosis of cervical intraepithelial neoplasia Herculano da Silva Martinho, Claudia Maria de Oliveira Monteiro da Silva, Maria Christina Botelho Mendonça Yassoyama, Patrícia de Oliveira Andrade, Renata Andrade Bitar, Ana Maria do Espírito Santo, Emilia A^ngela Loschiavo Arisawa, Airton Abrahão Martin Journal of Biomedical Optics, 2008
Raman study of human dentin irradiated with Er:YAG laser Luis E. S. Soares, Airton A. Martin, Aldo Brugnera, Jr., Fatima A. Zanin, Emilia A. Arisawa, Marcos T. T. Pacheco Progress in Biomedical Optics and Imaging Proceedings of SPIE, 2004
