Karina Maria Salvatore de Freitas

EDUCATION

DDS, MSc, PhD, Bauru Dental School, University of São Paulo

RESEARCH INTERESTS

Dentistry, Orthodontics, Dentofacial Orthopedics

Scopus Publications

Comparison of the efficiency of initial dental alignment with Invisalign® aligners changed every 7 or 14 days in mature adults: Randomized clinical trial
Tiago Fialho, Karina Maria Salvatore de Freitas, Eduardo Terumi Blatt Ohira, José Eduardo Prado de Souza, Renata Cristina Gobbi de Oliveira, Ricardo César Gobbi de Oliveira, Fabrício Pinelli Valarelli, Célia Regina Maio Pinzan‐Vercelino, and Paula Cotrin
Wiley
AbstractObjectiveTo compare the anterior crowding correction after sequential use of the first 5 aligners between two aligner exchange protocols (7 and 14 days) in mature adult patients.Materials and MethodsThirty‐six patients over 35 years who received orthodontic treatment with Invisalign® were randomly allocated to two different aligner replacement sequence protocols: Group 1: exchange every 7 days (n = 18); and Group 2: exchange every 14 days (n = 18). All patients were scanned with iTero Element 5D® (Align Technology) in two treatment times: at pre‐treatment (T1) and after using the first five aligners (T2). Little's Irregularity Index (LII) and arch widths were evaluated with OrthoCAD software. Intra and intergroup comparisons were performed with the dependent and independent t‐tests. The results were considered significant for P < .05.ResultsThirty‐five patients completed the study. Both groups' maxillary and mandibular Irregularity Indexesi were significantly lower at T2. There was no significant difference in inter‐canine, inter‐premolar, and intermolar distances. G2 (14 days) presented a greater decrease in mandibular irregularity than G1 (7 days).ConclusionBoth exchange protocols (7 and 14 days) effectively correct anterior crowding in the initial phase of orthodontic treatment with aligners in mature adult patients. However, the 14‐day exchange protocol provided a greater correction in mandibular anterior crowding in the evaluated period than the 7‐day exchange protocol.


Smile attractiveness in patients treated with or without 4 premolar extractions: A 36-year follow-up
Gabriela de Domênico Alcaraz Ros, Gabriel Eduardo Bravo Vallejo, Renan Morais Peloso, Caroline Martins Gambardela-Tkacz, Paula Cotrin, Karina Maria Salvatore de Freitas, and Marcos Roberto de Freitas
Elsevier BV



Comparison of maxillary transversal changes between auxiliary beta-titanium expansion arch and miniscrew-assisted rapid palatal expansion
Luis Henrique Braga Sader, Gustavo Siécola, Cecilia Maria Marin Ramirez, Aldo Otazu, Dino Marcelo Torres, Paula Cotrin, Fabrício Pinelli Valarelli, Célia Regina Maio Pinzan‐Vercelino, and Karina Maria Salvatore Freitas
Wiley
AbstractObjective(s)This study compared buccal bone thickness, dental inclinations and maxillary transverse width dimensions changes between auxiliary beta‐titanium expansion arch (AEA) and miniscrew‐assisted rapid palatal expansion (MARPE).Materials and MethodsThe sample consisted of 29 patients, aged between 18 and 40 years, with transverse maxillary deficiency and treated without extractions, divided into two groups: group AEA: comprised 13 individuals (initial mean age: 29.23 ± 9.13 years) treated using auxiliary beta‐titanium expansion arch; group MARPE: comprised 16 patients (initial mean age: 24.92 ± 7.60 years) treated with miniscrew‐assisted rapid palatal expansion. Buccal bone thickness, dental inclinations and transverse width dimensions (dental, nasal base and jugular) were measured in cone‐bean computed tomographies at pre‐ and post‐treatment stages. The variables were compared using the independent t‐test.ResultsThe buccal bone thickness was similar for both groups at the post‐treatment stage. The second premolars were significantly more buccal inclined in the AEA group and the right maxillary first molars in the MARPE group. The intercanine and intermolar distances were statistically significantly greater in the MARPE group. Changes in dental arch transverse dimensions were significantly greater for the MARPE group.ConclusionBoth treatment protocols corrected the maxillary transverse discrepancy; however, MARPE provided greater correction.


Long-term profile attractiveness of patients with Class I and II malocclusion treated with and without extractions: A 35-year follow-up
Gabriel Bravo Vallejo, Gabriela de Domênico Alcaraz Ros, Renan Morais Peloso, Caroline Martins Gambardela-Tkacz, Paula Cotrin, Karina Maria Salvatore Freitas, and Marcos Roberto de Freitas
Elsevier BV



Effect of poly-L-lactic acid and polydioxanone biostimulators on type I and III collagen biosynthesis
Rachel Thacyana Rorato Bernardo, Renata Cristina Gobbi de Oliveira, Karina Maria Salvatore de Freitas, José Ricardo de Albergaria‐Barbosa, and Célia Marisa Rizzatti‐Barbosa
Wiley
AbstractObjectiveSafe, effective, and biocompatible minimally invasive procedures with the potential to stimulate collagen production have been made to recover dermal thickness and skin quality. The main of this animal model experiment was to observe the effect of poly‐L‐lactic acid (PLLA) and polydioxanone (PDO) biostimulators in collagen I and III after hypodermal injection.MethodologySixteen adult female rats (Wistar) were randomized into four groups and had dorsal treatment with: G1: hypodermic subcision (HS) only; G2: HS and PLLA hypodermic injection (HI), G3: HS and PDO HI; G4: Control, with no treatment.ResultsIn histochemical, it was observed hypodermal and dermal tissue in more organized thickness in G3 and in G4 when compared to G1 and G2. There was few difference in G1 compared to G4. The tissue of G2 showed irregularities in the arrangement of collagen fibers, less defined structure and lower distribution of type I collagen compared to the other groups. There is a greater tendency for the proportions of type III collagen among tissues treated with both biostimulators (G2 and G3). PLLA and PDO had relatively similar percentages of collagen when compared to G4. The amount of type I collagen was higher in tissues treated with subcision, while type III collagen was higher in tissues treated with both biostimulators.ConclusionG3 showed better performance in collagen production, although small, when compared with G2.


Third Molar Comparison in Class I and II Extraction and Non-extraction Orthodontic Treatment: A Retrospective Longitudinal Study
Danelin Peña-Reyes, Jessica Quereza Freitas, Karina Maria Salvatore Freitas, Silvio Augusto Bellini-Pereira, Aron Aliaga-Del Castillo, Guilherme Janson, and Marcos Roberto Freitas
Galenos Yayinevi
Objective: This study compared third molar angulation and eruption status in Class I and II malocclusions after orthodontic treatment with and without first premolar extractions. Methods: The sample comprised 93 patients divided into four groups: Group 1, Class I malocclusion treated with first premolar extractions; Group 2, Class I malocclusion treated without extractions; Group 3, Class II malocclusion treated with first premolar extractions; and Group 4, Class II malocclusion treated without extractions. Panoramic radiographs were used to evaluate the third molar mesiodistal angulations at T1 (pretreatment), T2 (posttreatment), and T3 (long-term posttreatment). Third molar eruption status was assessed in dental casts. Intergroup angulations and eruption status comparisons were performed using one-way analysis of variance (ANOVA), followed by Tukey’s test and Kruskal-Wallis test, respectively. Results: Significantly greater mesial angulation and percentage of erupted right maxillary third molars were observed in the Class I extraction group. Significantly greater eruption status of the right mandibular third molars was observed in the Class I and Class II malocclusion extraction groups. Conclusion: Class I and II malocclusion extraction treatment exhibited more favorable angulations and a greater number of erupted third molars than non-extraction treatment. The non-extraction groups exhibited a greater percentage of unerupted third molars.


Comparative cephalometric study of the airways between different ethnic groups with normal occlusion
Genesis ZAMBRANO, Jose Gregorio Pelayo GUERRA, Laura Dias SOVIERO, Renan Morais PELOSO, Felicia MIRANDA, Karina Maria Salvatore de FREITAS, Arnaldo PINZAN, and Marcos Roberto de FREITAS
FapUNIFESP (SciELO)
ABSTRACT Objective: This study aimed to compare the nasopharynx and oropharynx airway dimensions of Caucasians, Blacks, Japanese, Japanese Brazilians, and Black Caucasians. Methods: A sample of 216 lateral radiographs of untreated young Brazilian subjects (mean age of 12.94 years; SD 0.88) were divided into five groups: Black Caucasian, Black, Caucasian, Japanese, and Japanese Brazilian. Lateral radiographs were used to measure the oropharynx (from the midpoint on the soft palate to the closest point on the anterior pharyngeal wall) and the nasopharynx (from the intersection of the posterior border of the tongue and the inferior border of the mandible to the closest point on the posterior pharyngeal wall). Analyses of variance (ANOVA) and Tukey’s test were performed (p< 0.05). Results: The linear dimension of the oropharynx was similar among the different ethnic groups. Caucasian individuals presented a significantly greater linear dimension of the nasopharynx than Black Caucasian and Black individuals. Conclusions: All the groups had similar buccopharyngeal values. However, Caucasian individuals had significantly higher values when compared to Black Caucasians and Black individuals.


Ozonated Water Promotes in vitro Decontamination of Dental Implants Surface
Renato Bitencourt Rosado, Eder José Cruz, Thainá Pinheiro de Souza, Karina Maria Salvatore Freitas, Mariana Aparecida Lopes Ortiz, and Samira Salmeron
Informa UK Limited



Comparison of Gingival Recession of Mandibular Incisors of Class III Patients Immediately after Compensatory or Surgical Orthodontic Treatment
Fábio Jorge Saab, Daniel Salvatore de Freitas, Paula Cotrin, Renata Cristina Oliveira, Fabricio Pinelli Valarelli, Ricardo Cesar Gobbi de Oliveira, Samira Salmeron, Célia Regina Maio Pinzan Vercelino, and Karina Maria Salvatore Freitas
Georg Thieme Verlag KG
Abstract Objective This study aimed to compare gingival recession in mandibular anterior teeth in patients with Class III malocclusion, immediately after compensatory or surgical orthodontic treatment. Materials and Methods The sample consisted of 40 patients with Class III malocclusion, divided into two groups: Group 1 (compensatory), 20 patients treated with compensatory orthodontics, with a mean initial age of 20.26 years (standard deviation [SD] . = 7.44), mean final age of 23.07 years (SD = 7.32), and mean treatment time of 2.81 years (SD =0.84). Group 2 (surgical), who undergone orthodontic–surgical treatment, with a mean initial age of 23.08 years (SD =5.48), mean final age of 25.43 years (SD =5.12), and mean treatment time of 2.35 years (SD =1.56). Intraoral photographs taken before and after removal of the fixed orthodontic appliance were used to measure the gingival recession, from the cervical of the mandibular incisors from the most cervical point of the gingival margin to the cementoenamel junction. In the initial and final cephalograms, the position of the mandibular incisors was measured. The intergroup comparison was performed using the independent t-test. Results The results showed that there was no statistically significant difference in the gingival recession at the beginning, at the end, and of changes with treatment between the compensatory and surgical groups. Conclusion It was concluded that the compensatory and surgical orthodontic treatments for Class III malocclusion showed similar results regarding the gingival recession of the mandibular incisors.


Evaluation of the attractiveness of lips with different volumes after filling with hyaluronic acid
Paula Martins de Queiroz Hernandez, Paula Cotrin, Fabricio Pinelli Valarelli, Ricardo Cesar Gobbi de Oliveira, Carina Gisele Costa Bispo, Karina Maria Salvatore Freitas, Renata Cristina Oliveira, and Dra. Paula Cotrin
Springer Science and Business Media LLC
AbstractTo compare the attractiveness of lips with different volumes after filling in the view of laypersons, dentists, and specialists. The sample comprised close-up frontal and lateral photographs of the lips of 16 women who underwent lip filling procedure with hyaluronic acid and was divided into 2 groups. Group 1: eight women with thinner lips at pretreatment. Group 2: eight female patients with thicker lips at pretreatment. Photographs from before and 10 days after lip filling were used to assess attractiveness randomly displayed in a Google Forms questionnaire and then sent via a messaging app to the evaluators. The evaluators' groups were general dentists, dentists with degrees in facial aesthetics, and laypersons. The attractiveness was evaluated with scores from 0 to 10 (0: least attractive and 10 the greatest). T-tests were used for the statistical comparisons. The group with thinner lips showed significantly improved attractiveness after filling. The group with thicker lips showed a worsening attractiveness after filling. The group with thicker lips had significantly higher attractiveness scores than those with thinner lips. There was no significant difference in the preference between men and women. The group of laypersons was more rigorous, giving significantly lower lip attractiveness scores. Thinner lips showed a significant improvement in attractiveness after filling. Thicker lips showed a worsening of the attractiveness score after filling. Before and after filling, thicker lips had significantly higher attractiveness scores than thinner lips.Clinical relevance: The amount of fillers applied to each patient must be individually evaluated.


Incisor irregularity and dental arch dimensions changes in subjects with different severity of anterior crowding: a 37-year follow-up
Caroline Martins Gambardela-Tkacz, Gabriela Alcaraz, Paula Cotrin, Karina Maria Salvatore de Freitas, Willana Moura, Guilherme Janson, Daniela Garib, and Marcos Roberto de Freitas
Springer Science and Business Media LLC
Abstract Background Occlusal stability is one of the goals of orthodontic treatment, and keeping teeth aligned in the long term is a challenge for the orthodontist. This study aimed to compare the long-term incisors irregularity and dental arches dimensions changes in subjects treated with 4 premolar extractions with different pretreatment Little's irregularity index (LII). The knowledge of long-term outcomes is evidence-based information for the prognosis of future treatments. Methods In total, 41 treated subjects were divided into two groups according to mandibular Little irregularity value at pretreatment (mild or severe). The maxillary and mandibular LII, transversal, and longitudinal widths were assessed at pretreatment, posttreatment, and 37-year posttreatment. Chi-square and independent t tests were used for intergroup comparison. Results The groups presented similar behavior for all stages of maxillary and mandibular arch dimensions changes. Maxillary irregularity was corrected in both groups after treatment, and the alignment was acceptable in the long term. In the mild group, the mandibular incisor irregularity returned to pretreatment values in the long term. The mandibular LII increased in the severe group but did not return to pretreatment values in the long term. Conclusions The mild crowding group presented proportionally more relapse of mandibular incisor irregularity than the severe crowding group in the long term. Even so, the correction of mild and severe crowding with the extraction of 4 premolars showed satisfactory results in the long term, even with the presence of maturational changes and relapse.


Maternal Risk Factors Associated with Negative COVID-19 Outcomes and Their Relation to Socioeconomic Indicators in Brazil
Helena Fiats Ribeiro, Maria Dalva de Barros Carvalho, Fernando Castilho Pelloso, Lander dos Santos, Marcela de Andrade Pereira Silva, Kely Paviani Stevanato, Deise Helena Pelloso Borghesan, Isaac Romani, Vlaudimir Dias Marques, Karina Maria Salvatore de Freitas,et al.
MDPI AG
Background: This study aimed to analyze maternal risk factors associated with negative outcomes of COVID-19 and association with socioeconomic indicators in Brazil. Methods: A cross-sectional study, with data from the Influenza Epidemiological Surveillance Information System (SIVEP-Flu) of pregnant women with COVID-19 and cases of hospitalization and death. For the analysis of risk factors and outcomes, the multiple logistic regression method was used. Results: Pregnant women who had some risk factor represented 47.04%. The chance of death was 2.48 times greater when there was a risk factor, 1.55 for ICU admission and 1.43 for use of ventilatory support. The percentage of cure was 79.64%, 15.46% without any negative outcome, 4.65% death and 0.26% death from other causes. Pregnant women who did not take the vaccine represented 30.08%, 16.74% took it and 53.18% were not specified. The variables HDI, illiteracy, per capita income and urbanization did not influence the cases of COVID-19. Conclusions: Factors such as age, obesity, asthma and pregnancy were responsible for the increase in hospitalizations, respiratory complications and death. Vaccination reduced the risk of negative outcomes by 50%. There were no correlations between socioeconomic indicators and the negative outcomes of COVID-19 in pregnant women.


Authors’ response
Cinthya Nogueira and Karina de Freitas
Elsevier BV



Mandibular asymmetry retreatment with minimal presurgical orthodontic preparation and fully customized lingual orthodontics: A case report with new possibilities using blender open-source software
Pedro Graziani Olímpio Pereira, Silvio Augusto Bellini-Pereira, Demi Dahás, Denis Pimenta e Souza, Karina Maria Salvatore Freitas, and Guilherme Janson
Elsevier BV



The Influence of Occlusal Adjustment on Long-term Post-treatment Stability of Orthodontic Treatment
Marcos Fernando Bonadio, Paula Cotrin, Cecília Maria Marín Ramirez, Tiago Fialho, Wilana da Silva Moura, Renata Cristina Gobbi de Oliveira, Ricardo Cesar Gobbi de Oliveira, Fabricio Pinelli Valarelli, Célia Regina Maio Pinzan-Vercelino, and Karina Maria Salvatore Freitas
Bentham Science Publishers Ltd.
Objective: This study aimed to compare the long-term occlusal stability in patients treated orthodontically with and without occlusal adjustment. Material and Methods: This observational study followed the STROBE guidelines. The sample comprised 77 patients with class I malocclusion treated without dental extractions and without signs and symptoms of temporomandibular joint dysfunction (TMD), divided into two groups. Group 1 with occlusal adjustment comprised 37 patients, 16 males and 21 females, with mean initial, final, and post-retention ages of 18.33 (s.d.=3.96), 20.99 (s.d.=3.33), and 25.79 years (s.d.=3.34), respectively. The treatment time was 2.66 years (s.d.=1.57), and the time of post-retention evaluation was 4.80 years (s.d.=1.10). Group 2, without occlusal adjustment involved 40 patients, 21 males and 19 females, with mean initial, final, and post-retention ages of 18.07 (s.d.=3.03), 20.75 (s.d.=3.54), and 25.89 (s.d.=3.73) years, respectively. The treatment time was 2.68 years (s.d.=1.09) and the time of post-retention evaluation was 5.14 years (s.d.=1.36). The PAR and Little irregularity indices were measured in the initial, final, and post-retention dental casts. Intergroup comparison was performed using the independent t-test. Results: There was no statistically significant difference observed in the intergroup comparison of the PAR and Little irregularity indexes in all phases and periods evaluated. Conclusion: There was no significant difference found in the long-term post-treatment crowding and occlusal relapse between class I patients treated with orthodontic non-extraction with and without occlusal adjustment. The occlusal adjustment did not influence the alignment and occlusal post-retention stability.


Incisors’ bone height and inclination changes after orthodontic treatment with a self-ligating passive system
C. Pinzan-Vercelino, K. Freitas, M. Secco, A. Pinzan, P. Cotrin, F. Valarelli, G. Janson, and M. Freitas
Medicina Oral, S.L.
Background This study aimed to evaluate changes in the alveolar buccal bone height of maxillary and mandibular incisors after orthodontic treatment with a self-ligating passive system and to assess the correlation between bone height and incisor inclination. Material and Methods Pre (T1) and post-treatment (T2) cone-beam computed tomography images of patients treated with the Damon 3MX appliance system were measured to quantify the alveolar buccal bone height of the maxillary incisors. The incisor’s inclination was measured in digital models. Paired t-test was used to evaluate the changes between T1 and T2, and Pearson’s coefficient was used to test the correlation. Results All teeth presented statistically significant alveolar buccal bone loss at T2. A statistically significant buccal inclination was observed only for the lower left lateral incisors. There was no correlation between bone height changes and incisor inclination. Conclusions Orthodontic treatment with a self-ligating passive system showed changes in alveolar height, but these changes were not correlated with incisor inclination. Key words:Passive self-ligating brackets, orthodontics, corrective, treatment outcome, alveolar bone loss.


Influence of the third molar presence on the thickness and height of the buccal cortical bone of the mandibular first and second molars
PM. Pinheiro, K. Freitas, and R. de Castro
Medicina Oral, S.L.
Background This study aimed to evaluate the influence of the presence of the third molars on the thickness and height of the buccal cortical bone of the first and second mandibular molars. Material and Methods The retrospective cross-sectional observational sample consisted of 102 CBCTs of patients (mean age of 29 years), divided into two groups: G1: 51 patients (26 female; 25 male, mean age of 26 years) presenting the mandibular third molars and G2: 51 patients (26 female; 25 male, mean age of 32 years) with the absence of the mandibular third molars. The total and the cortical depth were evaluated at 4 and 6mm from the cementoenamel junction (CEJ). The total thickness of the buccal bone was evaluated in two horizontal reference lines located apically 6 mm and 11 mm from the CEJ. Statistical comparisons were performed with Mann Whitney and Wilcoxon tests. Results In the comparison of buccal bone thickness and height between the groups, there was a statistical difference in tooth 36. In tooth 37 there was a statistical difference in the mesial root. For tooth 47, there was a statistical difference for the total thickness at 6mm, 11mm and 4mm. Concerning age, there was a tendency to decrease the values of these variables with increasing age. Conclusions The mean values for buccal bone thickness, total and cortical depth of the mandibular molars were higher for patients with mandibular third molars because the buccal bone thickness of the mandibular molars increased in the posterior and apical direction. Key words:Molar tooth, jaw, bone, orthodontic anchorage procedures, cone-beam computed tomography.


Correlation of age and skeletal effects after miniscrew assisted rapid palatal expansion
C. Marín, Md. Benitez, A. Otazu, D. Torres, P. Cotrin, C. Pinzan-Vercelino, F. Valarelli, and K. Freitas
Medicina Oral, S.L.
Background To evaluate the skeletal and dentoalveolar effects after miniscrew assisted rapid palatal expansion (MARPE) and their correlation with the age of the patients. Settings and sample population: Sample comprised 19 patients with maxillary atresia and posterior crossbite, treated with MARPE. Cone-beam computed tomographs (CBCT) were evaluated before and after expansion. Three patients were excluded since the midpalatal suture was not opened. Thus, 16 patients (11 female; 5 male) were evaluated, with a mean age of 24.92 years (s.d.=7.60). The time between the installation of MARPE and the second CBCT was, on average, 1.64 months (s.d.=1.12). Material and Methods Linear and angular measurements were performed: bone thickness and level, tooth inclination, transverse dental widths, and nasal base and jugula widths. Comparison was performed with dependent t-test and correlations with Pearson coefficient. Results MARPE was 84.2% successful. There was significant reduction in the buccal bone thickness of the first molars and an increase in the palatal bone thickness of all teeth. First molars showed significant buccal inclination. All transverse dimensions showed a significant increase. Older patients tended to show a less maxillary transverse skeletal increase. A greater maxillary transverse increase was accompanied by a greater intermolar width increase and also a greater buccal bone loss in the mesiobuccal roots of the maxillary first molars. Conclusions MARPE corrected the maxillary atresia in adult patients, with significant transverse increases, a slight decrease in buccal bone thickness and buccal inclination of the first molars, combining skeletal and dental effects. Older patients presented less transverse skeletal increases. Key words:Palatal expansion technique, skeletal anchorage, cone-beam computed tomography.


Authors’ response
Rosangela Colet, Paula Cotrin, Fabrício Pinelli Valarelli, and Karina Maria Salvatore Freitas
Elsevier BV



Evaluation of enamel deproteinization in bond strength of orthodontic accessories: A split-mouth randomized clinical trial
Renan Morais Peloso, Paula Cotrin, Renata Cristina Gobbi de Oliveira, Ricardo César Gobbi de Oliveira, Fabricio Pinelli Valarelli, and Karina Maria Salvatore Freitas
Elsevier BV



Gingival recession in mandibular anterior teeth in patients with Class II malocclusion treated with elastics and Twin Force appliance
Rosangela Colet, Paula Cotrin, Renata Cristina Oliveira, Fabricio Pinelli Valarelli, Ricardo Cesar Gobbi de Oliveira, Samira Salmeron, and Karina Maria Salvatore Freitas
Elsevier BV



Authors’ response
Roberta Caetano Calil, Fabricio Pinelli Valarelli, and Karina Maria Salvatore Freitas
Elsevier BV



Low-Level Laser and Antimicrobial Photodynamic Therapy Reduce Peri-implantitis-related Microorganisms Grown in Vitro
Marcelo H. Tonin, Fabiano C. Brites, José R. Mariano, Karina M. S. Freitas, Mariana A. L. Ortiz, and Samira Salmeron
Georg Thieme Verlag KG
Abstract Objective Currently, dental implants are a predictable treatment option for oral rehabilitation; however, complications such as peri-implant diseases are increasing every day. Thus, the aim of this study was to verify the efficacy, in vitro, of two protocols against cultures of periodontal biofilm and Staphylococcus aureus. Material and Methods Petri dishes for each of the following groups were used: control groups (C)—plates inoculated with periodontal biofilm (C.B; n = 4) or S. aureus (C.SA; n = 4) without any treatment; laser groups—plates inoculated with periodontal biofilm (low-level laser therapy [LLLT].B; n = 4) or S. aureus (LLLT.SA; n = 4) and treated with LLLT (660 nm, 30 mW, 50 J/cm2, and 47 seconds); antimicrobial photodynamic therapy groups (aPDT)—plates inoculated with periodontal biofilm (aPDT.B; n = 4) or S. aureus (aPDT.SA; n = 4) and treated with aPDT (red laser 660 nm, 30 mW, 50 J/cm2, 47 seconds + toluidine blue O (TBO) 100 µg/mL, and 1 minute). After treatments were performed, the contents of all plates were diluted and seeded for counting colony-forming units (CFUs). Statistical Analysis Results were analyzed with one-way analysis of variance (ANOVA), Tukey’s test, comparison of percentages, and independent t-tests with a 5% significance level. Results Both treatments, LLLT and aPDT, significantly reduced the number of CFUs for the two types of culture, LLLT.B (3.69 × 106 ± 0.20), aPDT.B (2.79 × 106 ± 0.13), LLLT.SA (4.10 × 106 ± 0.12), and aPDT.SA (3.23 × 106 ± 0.10) when compared with control groups C.B (5.18 × 106 ± 0.43) and C.SA (5.81 × 106 ± 0.16; p = 0.000). When treatment groups were compared separately, there was also a statistically significant difference (p = 0.000). None of the protocols were able to eliminate cultured microorganisms. Conclusion The LLLT and aPDT protocols effectively reduced cultures of periodontal biofilm and S. aureus in vitro, with the superiority of aPDT.


Class II treatment effects with fixed functional appliances: Jasper jumper vs. Forsus fatigue resistant device
Daniela Cubas Pupulim, José Fernando Castanha Henriques, Karina Maria Salvatore Freitas, Fernanda Pinelli Henriques Fontes, and Thais Maria Freire Fernandes
Orthodontics and Craniofacial Research Wiley
OBJECTIVE This study aimed to compare cephalometric changes of Class II malocclusion patients treated with Jasper Jumper and Forsus, associated with fixed appliances. METHODS The sample consisted of 62 individuals divided into 3 groups: group 1 included 22 subjects with a mean initial age of 12.39 years, treated with Jasper Jumper associated with fixed appliances for a mean period of 2.43 years; group 2 included 19 subjects with a mean initial age of 12.43 years, treated with Forsus associated with fixed appliances for a mean period of 3.54 years; and group 3 included 22 Class II malocclusion untreated subjects at a mean age of 12.14 years, followed for a mean period of 1.78 years. Intergroup comparison was performed with one-way ANOVA, followed by Tukey test. RESULTS Both treated groups had similar dentoskeletal changes: restrictive effect on the maxilla; clockwise rotation of the occlusal plane; mild increase in lower anterior facial height; limitation on the vertical development of the maxillary molars; labial tipping and intrusion of the mandibular incisors; extrusion of mandibular molars; improvements of the maxillomandibular relationship, overjet, overbite, molar relationship; and retrusion of the upper lip. The mandibular incisors exhibited greater protrusion in group 1 compared to the other groups. In addition, group 2 presented mild protrusion of the lower lip, and groups 1 and 3 showed mild retrusion. CONCLUSION The Jasper Jumper and Forsus appliances were effective and showed similar changes in the treatment of Class II malocclusion.


Preliminary Histological Evaluation of the Application of Ozone in the First Days of Orthodontic Force Induction in Animal Model
Melissa Faccini, Felipe Agostini, Tassio Drieu, Francisco Ubiratan Ferreira de Campos, Aguinaldo Garcez, Glauber Fabre Carinhena, Samira Salmeron, Ana Regina Casaroto, Fabricio Pinelli Valarelli, and Karina Maria Salvatore Freitas
Georg Thieme Verlag KG
Abstract Objectives The aim of the study is to histologically evaluate the effect of ozone therapy on orthodontic force induction in an animal model. Materials and Methods Twenty-four Wistar rats were divided into three groups (n = 8). A NiTi coil spring was installed from the maxillary first molar to the maxillary central incisor. G1 was control and G2/G3 received 1 mL of ozonated gas at concentrations of 10 and 60 µg/mL, in the buccal mucosa above the first molar roots. The animals were euthanized 3 and 5 days after the procedure. Histological sections were obtained, longitudinally of the first molar’ long axis, in the mesiodistal direction. The number of osteoclasts, osteoblasts, blood vessels, polymorphonuclear and mononuclear cells, formation of osteoid tissue and hyaline areas, and root resorption were evaluated with light microscope, in tension and pressure sides. Intergroup comparisons were performed with Kruskal–Wallis, Dunn, and Chi-square tests. Results At 3-days pressure side, a greater number of osteoclasts was observed in ozone groups and greater number of blood vessels and polymorphonuclear cells were observed in G2. On the tension side, there was a significantly greater number of blood vessels, osteoblasts, and mononuclear cells in G2. At 5-days pressure side, there was a significantly greater number of osteoclasts in G2, blood vessels and osteoblasts in the ozone groups, and lesser number of polymorphonuclear cells in G3. Conclusion Ozone therapy increased the number of osteoclasts on the pressure side and osteoblasts on tension side, in 10 µg/mL concentration, demonstrating histological parameters favorable to bone remodeling. The 60 µg/mL ozone concentration accelerated the periodontal ligament reorganization process.

RECENT SCHOLAR PUBLICATIONS

Comparison of facial profile attractiveness in pattern III patients treated with facial filling or orthognathic surgery
FR Cunha, T Fialho, JEP de Souza, DS Freitas, P Cotrin, ...
Journal of Cranio-Maxillofacial Surgery 53 (3), 239-244 2025



The Impact of the COVID-19 Pandemic on SUS (Universal Health System) Primary Preventive Dental Care in Brazil during the First Two Years of the Pandemic
AC CAPRIGLIONE, T FIALHO, ETB OHIRA, AL PONCE, ALP PEREIRA, ...
Journal of Advances in Medicine and Medical Research 37 (3), 38-47 2025



Extreme dentoalveolar compensation in complex skeletal Class III malocclusion with anterior open bite and total crossbite
E Prado, FP Valarelli, KMS Freitas
AJO-DO Clinical Companion 2025



Manejo esttico inovador da m ocluso de Classe III em adultos, usando Carriere Motion 3D Clear e Invisalign: relato de caso.
E PRADO, F Pinelli VALARELLI, P COTRIN, KM Salvatore FREITAS
Clinical Orthodontics 24 (1) 2025



Mesializao de molares com mini-implantes como abordagem alternativa para o tratamento de segundos pr-molares inferiores ausentes: relato de caso.
G de Domnico Alcaraz ROS, B QUEVEDO, KM Salvatore de FREITAS, ...
Clinical Orthodontics 24 (1) 2025



Surgical and Camouflage Orthodontic Treatment of Anterior Open Bite: Comparison of Profile Attractiveness
CQ Nogueira, P Cotrin, DS Freitas, JE Prado de Souza, FP Valarelli, ...
Orthodontics & Craniofacial Research 28 (1), 187-195 2025



Strategic maxillary second molar extraction in Class II malocclusion: A 22-year follow-up update
DV de Lima, MR de Freitas, KMS Freitas
AJO-DO Clinical Companion 5 (1), 127-129 2025



Retention preferences and protocols of Brazilian orthodontists: a cross-sectional study
DG Machado, DO SILVA, JA GURGEL, RDR MENDONA, P Cotrin, ...
Dental Press Journal of Orthodontics 29 (6), e2423251 2024



Effects of the passive self-ligating system on alveolar bone thickness, dental inclinations, and arch dimensions
M Matias, B Vieira, KMS Freitas, CRM Pinzan-Vercelino, P Cotrin, ...
Journal of Clinical and Experimental Dentistry 16 (11), e1361 2024



Comparison of aging of nonextraction treated and normal untreated occlusions: 40-year follow up
KMS Freitas, C Massaro, F Miranda, CM Gambardela-Tkacz, P Cotrin, ...
Scientific Reports 14 (1), 25458 2024



Fechamento do espao de incisivos laterais superiores ausentes bilateralmente em paciente Classe III: caso clnico.
R Alex IWASAKI, J MENEGUSSI, T FIALHO, KM Salvatore de FREITAS, ...
Clinical Orthodontics 23 (5) 2024



Patients’ perceptions matter: Risk communication and psychosocial factors in orthodontics
Y Zhao, X Shi, J Liu, R Huo, K Xia, Y Wang, G Zhu, W Lu, L Zhang, ...
American Journal of Orthodontics and Dentofacial Orthopedics 166 (4), 393 2024



Long-term profile attractiveness of patients with Class I and Class II malocclusion treated with or without extractions: Overlooked insights? Response
GB Vallejo, GDA Ros, RM Peloso, CM Gambardela-Tkacz, P Cotrin, ...
AMERICAN JOURNAL OF ORTHODONTICS AND DENTOFACIAL ORTHOPEDICS 166 (3), 197-198 2024



Comparison of smile attractiveness in cases with gummy smile treated with botulinum toxin and maxillary impaction surgery: A retrospective study
DB de Menezes Borba, T Fialho, RCG de Oliveira, RCG de Oliveira, ...
Journal of Cranio-Maxillofacial Surgery 52 (9), 999-1005 2024



Assessment of patients’ knowledge and preferences for the use of orthodontic aligners
J Paim, LFD Souza, T Fialho, DBM Borba, KMS Freitas, P Cotrin, VB Neis, ...
Journal of Orthodontics 51 (3), 251-257 2024



Invisalign para tratamento de mordida aberta anterior.
JE Prado de SOUZA, T Stefhanie de ALMEIDA, D Moreira PROCPIO, ...
Clinical Orthodontics 23 (4) 2024



Comparison of the efficiency of initial dental alignment with Invisalign aligners changed every 7 or 14 days in mature adults: Randomized clinical trial
T Fialho, KMS de Freitas, ETB Ohira, JEP de Souza, RCG de Oliveira, ...
Orthodontics & Craniofacial Research 27 (4), 544-551 2024



Long-term Evaluation (10 Years) of the Outcomes of Herbst Functional Appliance in Pharyngeal Dimensions and Hyoid Bone Position in the Treatment of Class II Patients
TYL Almeida, T Fialho, KMS de Freitas, JFC Henriques, MR de Freitas
2024



Comparison of speech changes caused by four different orthodontic retainers: a crossover randomized clinical trial
DC Lorenzoni, JFC Henriques, LK Silva, RR Rosa, G Berretin-Felix, ...
Dental Press Journal of Orthodontics 29 (3), e2423277 2024



COMPARISON OF LIGHT-CURING TIME WITH THE USE OF DIFFERENT LED INTENSITIES IN THE BONDING OF ORTHODONTIC BRACKETS
BM Ceribelli, P Cotrin, RH Canado, FP Valarelli, KMS de Freitas
Revista de Cincias Mdicas e Biolgicas 23 (1), 62-66 2024

MOST CITED SCHOLAR PUBLICATIONS

Upper and lower pharyngeal airways in subjects with Class I and Class II malocclusions and different growth patterns
MR de Freitas, NMPV Alcazar, G Janson, KMS de Freitas, JFC Henriques
American journal of orthodontics and dentofacial orthopedics 130 (6), 742-745 2006
Citations: 316



How does the quarantine resulting from COVID-19 impact dental appointments and patient anxiety levels?
RM Peloso, NIP Pini, D Sundfeld Neto, AA Mori, RCG Oliveira, ...
Brazilian oral research 34, e84 2020
Citations: 163



Impact of coronavirus pandemic in appointments and anxiety/concerns of patients regarding orthodontic treatment
P Cotrin, RM Peloso, RC Oliveira, RCG de Oliveira, NIP Pini, FP Valarelli, ...
Orthodontics & craniofacial research 23 (4), 455-461 2020
Citations: 162



COVID-19 pandemic and pediatric dentistry: Fear, eating habits and parent’s oral health perceptions
R Campagnaro, G de Oliveira Collet, MP de Andrade, JPSL Salles, ...
Children and youth services review 118, 105469 2020
Citations: 153



Notes from the field: concerns of health-related higher education students in Brazil pertaining to distance learning during the coronavirus pandemic
RM Peloso, F Ferruzzi, AA Mori, DP Camacho, LCS Franzin, ...
Evaluation & the Health Professions 43 (3), 201-203 2020
Citations: 132



Postretention relapse of mandibular anterior crowding in patients treated without mandibular premolar extraction
KMS Freitas, MR de Freitas, JFC Henriques, A Pinzan, G Janson
American journal of orthodontics and dentofacial orthopedics 125 (4), 480-487 2004
Citations: 129



Comparative distalization effects of Jones jig and pendulum appliances
MP Patel, G Janson, JFC Henriques, RR de Almeida, MR de Freitas, ...
American Journal of Orthodontics and Dentofacial Orthopedics 135 (3), 336-342 2009
Citations: 127



Healthcare workers in Brazil during the COVID-19 pandemic: a cross-sectional online survey
P Cotrin, W Moura, CM Gambardela-Tkacz, FC Pelloso, L Santos, ...
INQUIRY: The Journal of Health Care Organization, Provision, and Financing 2020
Citations: 124



Prevalncia das ms ocluses em pacientes inscritos para tratamento ortodntico na Faculdade de Odontologia de Bauru-USP
MR Freitas, DS Freitas, F Pinheiro, KMS Freitas
Rev Fac Odontol Bauru 10 (3), 164-9 2002
Citations: 107



Influence of the quality of the finished occlusion on postretention occlusal relapse
KMS de Freitas, G Janson, MR de Freitas, A Pinzan, JFC Henriques, ...
American Journal of Orthodontics and Dentofacial Orthopedics 132 (4), 428 2007
Citations: 104



Dental care during COVID-19 outbreak: A web-based survey
M Faccini, F Ferruzzi, AA Mori, GC Santin, RC Oliveira, RCG de Oliveira, ...
European journal of dentistry 14 (S 01), S14-S19 2020
Citations: 97



Stability of anterior open-bite treatment with occlusal adjustment
G Janson, MV Crepaldi, KMS Freitas, MR de Freitas, W Janson
American journal of orthodontics and dentofacial orthopedics 138 (1), 14. e1 2010
Citations: 82



A comparison of skeletal, dentoalveolar and soft tissue characteristics in white and black Brazilian subjects
LMA Freitas, KMS Freitas, A Pinzan, G Janson, MR Freitas
Journal of Applied Oral Science 18, 135-142 2010
Citations: 79



Evaluation of maxillary buccal alveolar bone before and after orthodontic alignment without extractions: A cone beam computed tomographic study
JF Morais, B Melsen, KMS de Freitas, N Castello Branco, DG Garib, ...
The Angle Orthodontist 88 (6), 748-756 2018
Citations: 74



Predisposing factors to severe external root resorption associated to orthodontic treatment
GV Picano, KMS Freitas, RH Canado, FP Valarelli, PRB Picano, ...
Dental press journal of orthodontics 18, 110-120 2013
Citations: 74



Urgencies and emergencies in orthodontics during the coronavirus disease 2019 pandemic: Brazilian orthodontists' experience
P Cotrin, RM Peloso, NIP Pini, RC Oliveira, RCG de Oliveira, FP Valarelli, ...
American journal of orthodontics and dentofacial orthopedics 158 (5), 661-667 2020
Citations: 65



Cephalometric characterization of skeletal Class II, division 1 malocclusion in white Brazilian subjects
MR Freitas, MAC Santos, KMS Freitas, G Janson, DS Freitas, ...
Journal of Applied Oral Science 13, 198-203 2005
Citations: 65



Postretention stability after orthodontic closure of maxillary interincisor diastemas
JF Morais, MR Freitas, KMS Freitas, G Janson, N Castello Branco
Journal of Applied Oral Science 22 (05), 409-415 2014
Citations: 59



Factors associated to apical root resorption after orthodontic treatment
JDV Pastro, ACA Nogueira, KMS de Freitas, FP Valarelli, RH Canado, ...
The open dentistry journal 12, 331 2018
Citations: 58



Facial height comparison in young white and black Brazilian subjects with normal occlusion
LMA de Freitas, A Pinzan, G Janson, KMS Freitas, MR de Freitas, ...
American Journal of Orthodontics and Dentofacial Orthopedics 131 (6), 706 2007
Citations: 58