Ibiyeye Kehinde Muibat

I am interested in Cell and Cancer Biology. My interest is in understanding the uniqueness of Africans that contributes to the clinical presentation and outcome of cancer particularly breast and gynecological cancers. I am currently a lecturer/researcher in the Department of Anatomy

EDUCATION

MBBS, PhD

RESEARCH, TEACHING, or OTHER INTERESTS

Cell Biology, Cancer Research, Anatomy

Scopus Publications

Cytopathology Practice in Nigeria
Olaleke O. Folaranmi, Oladunni I. Olayiwola, Kehinde M. Ibiyeye, Mikhail O. Buhari, Olatunde K. Ibrahim, Ehigie J. Ighodalo, and Abdulquadri Balogun
Wiley
ABSTRACTCytopathology is a cost‐effective diagnostic method crucial for investigating suspected neoplastic lesions, particularly in Nigeria's healthcare system, where non‐communicable diseases, especially with increasing rates of cancer. This review highlights the evolution of cytopathology practice in Nigeria, detailing its historical development, current methodologies, and the challenges faced within the healthcare framework. Despite advancements in diagnostic techniques such as immunocytochemistry and molecular diagnostics globally, conventional methods remain essential due to their affordability and accessibility. Fine needle aspiration cytology (FNAC) has gained acceptance for its diagnostic accuracy; however, significant disparities exist in the availability of cytological services across various regions. The lack of trained personnel and inadequate infrastructure further complicate the effective implementation of cytopathology practices. The findings highlight the urgent need for specialized training programs and improved resource allocation to enhance cytopathology services in Nigeria. We advocate for strategic interventions aimed at optimizing cytopathology's potential to improve cancer diagnosis and patient outcomes in resource‐limited settings across the country.


Changing pattern of cancer distribution: Experience from a tertiary health institution in Nigeria and review of literature
KA Adeniji, OO Folaranmi, OA Odetunde, O Adegboye, KM Ibiyeye, JE Ighodalo, IO Olayiwola, KA Suleiman, and SK Abubakar-Akanbi
Medknow
ABSTRACT Background: Over the years, epidemiological surveys have established that the five leading cancers in Africa (in descending order of prevalence) are cancers of the breast, cervix, prostate, liver, and colorectum.[1] However, a 10-year retrospective review of cases performed at the University of Ilorin Teaching Hospital (UITH) revealed some changes in the cancer incidence pattern in this region. Aims: The aim of the study was to determine the distribution of cancers managed at the UITH, a tertiary health care center in North Central Nigeria, in the past 10 years and create awareness of changing cancer prevalence patterns in this region. Materials and Method: This was a retrospective review of cancer epidemiology at UITH over a period of 10 years. Confirmed cancer cases within this period were extracted from the data in the Department of Anatomic Pathology and the Cancer registry. Descriptive and inferential statistics were applied to obtain rates and proportions for both sexes. Results: There were 2430 confirmed cases of cancer during the study period from January 2011 to December 2020. Out of these cases, 1310 (54%) were seen in females and 1120 (46%) were seen in males. The most common cancer recorded (in total) was prostate cancer, which accounted for 18% of all cases, constituting approximately one in six of all cancer cases. This was followed closely by breast cancer (16.6%). The most commonly diagnosed cancer in males was prostate cancer (four in ten cancer cases in males), whereas in females, it was breast cancer (approximately three in ten cancer cases in females). Cancers of the lungs and liver were rare in both sexes in this study. In children, the most common malignancies were retinoblastoma (38.3%), non-Hodgkin lymphomas (16.8%), and nephroblastoma (12.8%). Conclusion: There is a changing trend in cancer cases with some cancer cases now predominating compared to previous years. This may be because of increased awareness and/or better medical screening and diagnostic techniques.


The Influence of Social Media in Promoting Knowledge Acquisition and Pathology Excellence in Nigeria
Olaleke Oluwasegun Folaranmi, Kehinde Muibat Ibiyeye, Olabode Ali Odetunde, and Darcy A. Kerr
Frontiers Media SA
The use of social media has evolved from platforms designed primarily for social connection and news sharing to include vibrant virtual academic environments. These platforms allow pathologists from across the globe to interact, exchange knowledge, and collaborate. Pathology in Nigeria, as in much of Africa, faces severe knowledge and practice gaps, with a lack of supporting modern laboratory infrastructure. Social media represents a potentially highly valuable avenue to help address some of these deficiencies. In this Perspective piece, we highlight our experience with the increasing role of social media in providing quality medical education in pathology globally, with an emphasis on how it bridges many of these gaps in Nigeria. Social media sites serve as sources of readily accessible, free, high-quality information to pathologists and trainees through academic discussions, quizzes, journal clubs, and informal consultations. They also provide opportunities for professional networking and research collaborations. Despite the availability and wide reach of these platforms, social media as a tool for advancement of knowledge in pathology is still undersubscribed in this part of the world. Improving awareness of and support for these tools will ideally help mitigate some of the challenges of practicing pathology in low and middle-income settings.


Cockle shell-derived aragonite calcium carbonate nanoparticle for targeting cancer and breast cancer stem cells
Kehinde Muibat Ibiyeye, Sherifat Banke Idris, and Abu Bakar Zakaria Zuki
Springer Science and Business Media LLC
AbstractCockle shell-derived aragonite calcium carbonate nanoparticles (CACNP) have demonstrated prospect as nano-sized drug carriers for targeting cancer cells. CACNP is biocompatible, biodegradable and its biomaterial is readily available and is of low cost. In addition, CACNP is highly porous, has a large surface area which confer a high loading capacity. The pH-dependent release properties as well as its potential for surface functionalization with targeting agents make CACNP useful in passive and active targeting of cancer cells and cancer stem cells. In this article, we reviewed the current state of CACNP as nano-sized drug carrier for targeting cancer cells, cancer stem cells and its biocompatibility.


Cockle shell-derived aragonite CaCO3 nanoparticles for co-delivery of doxorubicin and thymoquinone eliminates cancer stem cells
Kehinde Muibat Ibiyeye and Abu Bakar Zakaria Zuki
MDPI AG
Cancer stem cells CSCs (tumour-initiating cells) are responsible for cancer metastasis and recurrence associated with resistance to conventional chemotherapy. This study generated MBA MD231 3D cancer stem cells enriched spheroids in serum-free conditions and evaluated the influence of combined doxorubicin/thymoquinone-loaded cockle-shell-derived aragonite calcium carbonate nanoparticles. Single loaded drugs and free drugs were also evaluated. WST assay, sphere forming assay, ALDH activity analysis, Surface marker of CD44 and CD24 expression, apoptosis with Annexin V-PI kit, cell cycle analysis, morphological changes using a phase contrast light microscope, scanning electron microscopy, invasion assay and migration assay were carried out; The combination therapy showed enhanced apoptosis, reduction in ALDH activity and expression of CD44 and CD24 surface maker, reduction in cellular migration and invasion, inhibition of 3D sphere formation when compared to the free drugs and the single drug-loaded nanoparticle. Scanning electron microscopy showed poor spheroid formation, cell membrane blebbing, presence of cell shrinkage, distortion in the spheroid architecture; and the results from this study showed that combined drug-loaded cockle-shell-derived aragonite calcium carbonate nanoparticles can efficiently destroy the breast CSCs compared to single drug-loaded nanoparticle and a simple mixture of doxorubicin and thymoquinone.


Combine drug delivery of thymoquinone-doxorubicin by cockle shell-derived ph-sensitive aragonite CaCO<inf>3</inf> nanoparticles
Kehinde M. Ibiyeye, Abu B.Z. Zuki, Norshariza Nurdin, and Mokrish Ajat
Bentham Science Publishers Ltd.
Background: Cockleshell-derived aragonite calcium carbonate nanoparticles were prepared by the top-down approach for combine delivery of two types of drugs. Objective: The aim of this study was to synthesize and characterize thymoquinone-doxorubicin loaded cockle shell-derived aragonite calcium carbonate nanoparticle. Aragonite calcium carbonate nanoparticles encapsulating thymoquinone and doxorubicin alone were also prepared. Methods: The blank and drug-loaded nanoparticles were characterized by field emission scanning electron microscopy, transmission electron microscopy, Zeta potential, Fourier transformed infrared and X-ray diffraction. Drug delivery properties, in vitro drug release study at pH 7.4, 6 and 4.8, and effect of blank nanoparticles on MCF10A, 3T3, MDA MB231 cells were also analyzed. Results: The blank and drug-loaded nanoparticles were pleomorphic and their sizes varying from 53.65 ± 10.29 nm to 60.49 ± 11.36 nm with an overall negative charge. The entrapment efficiency of thymoquinone and doxorubicin were 41.6 and 95.8, respectively. The FTIR showed little alteration after loading thymoquinone and doxorubicin while XRD patterns revealed no changes in the crystallizations of nanoparticles after drug loading. The drug release kinetics of doxorubicin and thymoquinone from the nanoparticles showed a continuous and gradual release after an initial burst release was observed. At pH 4.8, about 100% of drug release was noticed, 70% at pH 6 while only 50% at pH 7.4. The cell viability was 80% at a concentration of 1000 ug/ml of blank nanoparticle. Conclusion: The cockle shell-derived pH sensitive aragonite calcium carbonate nanoparticle provides an effective and simple means of multiple drug delivery and function as a platform for pH controlled release of loaded therapeutic agents.


Ultrastructural Changes and Antitumor Effects of Doxorubicin/Thymoquinone-Loaded CaCO<inf>3</inf> Nanoparticles on Breast Cancer Cell Line
Kehinde Muibat Ibiyeye, Norshariza Nordin, Mokrish Ajat, and Abu Bakar Zakaria Zuki
Frontiers Media SA
Background: Combination chemotherapy of anticancer drugs is extensively being researched since it could reduce multidrug resistance and side effects as a result of lower dosage of each drug. In this study, we evaluated the effects of doxorubicin-loaded (Dox-ACNP), thymoquinone-loaded (TQ-ACNP) and a combined doxorubicin/thymoquinone-loaded cockle shell-derived aragonite calcium carbonate nanoparticles (Dox/TQ-ACNP) on breast cancer cell line and compared with their free drugs counterpart. Methods: Cell viability using MTT assay, apoptosis with Annexin V-PI kit, morphological changes using contrast light microscope, scanning electron microscope and transmission electron microscope, cell cycle analysis, invasion assay, and scratch assay were carried out. The cell viability was evaluated in breast cancer cell line (MDA MB231), normal breast cells (MDF10A) and normal fibroblast (3T3). Results: MDA MB231 IC50 dosages of drug-loaded nanoparticle were not toxic to the normal cells. The combination therapy showed enhanced apoptosis, reduction in cellular migration and invasion when compared to the single drug-loaded nanoparticle and the free drugs. Scanning electron microscope showed presence of cell shrinkage, cell membrane blebbing, while transmission electron microscope showed nuclear fragmentation, disruption of cell membrane, apoptotic bodies, and disruption of mitochondrial cistern. Conclusion: The results from this study showed that the combined drug-loaded cockle shell-derived aragonite calcium carbonate nanoparticles (Dox/TQ-ACNP) showed higher efficacy in breast cancer cells at lower dose of doxorubicin and thymoquinone.

RECENT SCHOLAR PUBLICATIONS