Eman Mahmoud Abu El-Naga
@aswu.edu.eg
Theriogenology department , veterinary Medicine college
Aswan University
Scopus Publications
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Scopus Publications
Ahmed Ateya, Fatmah A. Safhi, Huda El-Emam, Muath Q. Al-Ghadi, Mohamed Abdo, Liana Fericean, Rada Olga, Ostan Mihaela, Manar M. Hizam, Maha Mamdouh,et al.
MDPI AG
The immunological genes that may interact with inflammatory postpartum diseases in Italian buffaloes were examined in this study. A total number of 120 female Italian buffaloes (60 normal and 60 with inflammatory reproductive diseases) were employed. Each buffalo’s jugular vein was pierced to get five milliliters of blood. To obtain whole blood and extract DNA and RNA, the blood was placed within tubes containing sodium fluoride or EDTA anticoagulants. The immunological (IKBKG, LGALS, IL1B, CCL2, RANTES, MASP2, HMGB1, and S-LZ) genes’ nucleotide sequence differences between healthy buffaloes and buffaloes affected by inflammatory reproductive diseases were found by employing PCR-DNA sequencing. According to Fisher’s exact test (p ˂ 0.01), there were noticeably different probabilities of all major nucleotide changes spreading among buffalo groups with and without reproductive problems. Buffaloes were significantly more likely to express the examined genes when they had inflammatory reproductive diseases. The outcomes might support the significance of these markers’ nucleotide variations and gene expression patterns as indicators of the prevalence of inflammatory reproductive disorders and provide a workable buffalo management policy.
Yahia A. Amin, Eman M. Abu El- Naga, Enas A. Noseer, Samer S. Fouad, and Rana A. Ali
Elsevier BV
During the summer season, high ambient temperature in tropical and subtropical countries exposes buffaloes to oxidative stress that have antigonadotropic and antisteroidogenic effects. Uses of hormonal therapy can improve the state of ovarian inactivity caused by heat stress and cause anoestrous buffaloes to come into oestrus and successfully achieve pregnancy. Therefore, this study was designed to detect the role of oxidative stress in production of the anoestrous state in summer heat stressed buffaloes and the effects of treatment by Controlled internal drug release (CIDR) in solving of this problem. Also it monitored the changes in Oxidant/antioxidant biomarkers and mineral profile before and after the treatment. Forty buffaloes with no signs of oestrus for more than 120 days after calving were selected. The animals were divided into two groups: the first group (group I, n = 25) was subjected to treatment with CIDR, while the second group (group II) received no treatment and was considered the control group (n = 25). Blood samples were collected before treatment, after treatment and after 45 days of oestrus. The serum level of TAC, MDA, NO, ascorbic acid (vitamin C), P, Cu and Zn were measured. The results showed that 80% of treated buffaloes came into oestrus. The conception rate was 75%. TAC concentrations were significantly higher in group I than in group II. There were significant decreases in the mean values of MDA, NO and ascorbic acid in the buffaloes in oestrus, but these values increased when the buffaloes became pregnant. In contrast there were no significant differences in the mean values of MDA, NO or ascorbic acid in the buffaloes that came into oestrus but failed to conceive. The mean serum P, Cu and Zn values were significantly increased (P < 0.05) in the buffaloes that came into oestrus compared to the control animals. The levels of P and Zn significantly increased when the buffaloes became pregnant and remained unchanged when they failed to conceive. In conclusion, known physiological and pathological variations in the oxidant/antioxidant parameters and mineral profile of summer anoestrous buffaloes may help to understand this problem of infertility.
A.M. Hammam, C.S. Whisnant, A. Elias, S.M. Zaabel, A.O. Hegab, and E.M. Abu-El Nag
Medwell Publications