CHANDRASHEKHAR V M
@bvvs-hskcop.ac.in
PROFESSOR IN PHARMACOLOGY
BVVS HANAGAL SHRI KUMARESHWAR COLLEGE OF PHARMACY BAGALKOT
RESEARCH, TEACHING, or OTHER INTERESTS
Pharmacology, Toxicology and Pharmaceutics, Pharmacology, Drug Discovery, Toxicology
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
Mallappa H. Shalavadi, V.M. Chandrashekhar, and I.S. Muchchandi
Elsevier BV
Rekha S. Hunoor, Basavaraj R. Patil, Dayananda S. Badiger, Chandrashekhar V. M., I. S. Muchchandi, and Kalagouda B. Gudasi
Wiley
This article describes the synthesis, structural aspects and biological studies of Co(II), Ni(II), Cu(II) and Zn(II) complexes of a new hydrazone derived from the condensation of isatin and 2‐aminobenzoylhydrazide. The ligand is well characterized using 1H NMR, 13C NMR, 2D HETCOR, mass and IR spectral studies. The chelating tendency of the ligand towards transition metal ions is established using analytical and spectral studies, which reveal the monobasic tridentate nature of the ligand. Octahedral geometry for Co(II), Cu(II) and Zn(II) and tetrahedral geometry for Ni(II) are tentatively proposed. All the synthesized compounds were screened for in vitro anticancer activity against Ehrlich ascites carcinoma and human cancer cell lines (adenocarcinoma HT29, kidney cancer cell line K293 and breast cancer cell line MDA231) using tryphan blue exclusion method and MTT assay. Copyright © 2014 John Wiley & Sons, Ltd.
VM Chandrashekhar, S Ganapaty, A Ramkishan, and MLaxmi Narsu
Medknow
Objectives: The objective of this study is to evaluate the neuroprotective effect of gossypin (isolated from Hibiscus vitifolius) against global cerebral ischemia/reperfusion (I/R) injury-induced oxidative stress in rats. Materials and Methods: Sprague Dawlet rats of wither gender were used in the study. Evaluation of cerbroprotective activity of bioflavonoid gossypin (in 5, 10 and 20 mg/kg oral doses) isolated from H. vitifolius was carried out by using the global cerebral I/R model by bilateral carotid artery occlusion for 30 min, followed by 24 h reperfusion. The antioxidant enzymatic and non-enzymatic levels were estimated along with histopathological studies. Result: Gossypin showed dose-dependent neuroprotective activity by significant decrease in lipid peroxidation (P < 0.001) and increase in the superoxide dismutase, catalase, glutathione and total thiol levels in gossypin treated groups when compared to control group. Cerebral infarction area was markedly reduced in gossypin treated groups when compared to control group. Conclusion: Gossypin showed potent neuroprotective activity against global cerebral I/R injury-induced oxidative stress in rats.
M. H. Shalavadi, V. M. Chandrashekhar, A. Ramkishan, R. B. Nidavani, and B. S. Biradar
Informa UK Limited
Abstract Context: Stereospermum suaveolens DC. (Bignoniaceae) is a medicinal tree species native to India. Traditionally, the whole plant is used for various diseases including neuronal disorders. Objective: The present study evaluated the neuroprotective activity of Stereospermum suaveolens against global cerebral ischemia in a rat model. Materials and methods: Neuroprotective activity was carried out by global cerebral ischemia on Sprague-Dawley rats and divided into five groups of eight rats each; sham and control groups received normal saline (10 ml/kg) and treated groups received methanol extract of Stereospermum suaveolens (MES) orally (125, 250, and 500 mg/kg) for 10 days prior to the experiment. Global cerebral ischemia was induced by bilateral carotid artery (BCA) occlusion for 30 min followed by 4-h reperfusion. The antioxidant enzymatic and non-enzymatic levels were estimated by UV spectroscopic method along with cerebral infarction area; histopathological studies were carried out. Results: LD50 of MES was found to be 5000 mg/kg of body weight. The entire test was performed at dose levels 125, 250, and 500 mg/kg of body weight. The results of the study indicate that the Stereospermum suaveolens methanol extract showed neuroprotective activity by a significant decrease in lipid peroxidation (p < 0.001) and an increase in superoxide dismutase (p < 0.01), catalase (p < 0.01), glutathione (p < 0.001), and total thiol (p < 0.001) levels in extract-treated groups as compared to control group. Measurement of cerebral infarction area and histopathological studies further supported the protective effect of the extract. Discussion and conclusion: These findings suggest a potential protective role of Stereospermum suaveolens against global cerebral ischemia/reperfusion-induced brain injury.
Alkesh Patel, S. Rajesh, V.M. Chandrashekhar, Shivprakash Rathnam, Karishma Shah, C. Mallikarjuna Rao, and K. Nandakumar
Elsevier BV
Ravindra G. Kulkarni, Stefan A. Laufer, Chandrashekhar V. M, and Achaiah Garlapati
Bentham Science Publishers Ltd.
P38 mitogen activated protein kinases have been found to involve in the production and release of unwarranted levels of pro-inflammatory cytokines including TNFα and IL-1β in numerous inflammatory diseases. A new series of molecules, 5-substituted benzoylamino-2-substituted phenylbezimidazoles has been synthesized from 4-nitro-1, 2- diaminobenzene. The synthesized compounds were characterized by FTIR, 1HNMR and Mass. The final compounds were screened for in vitro p38 kinase inhibitory and in vivo anti-inflammatory activity. Three compounds from the series demonstrated nearly 50% inhibition of p38 kinase in the in vitro screening method at 10 μM concentration and two molecules exhibited greater than 75% inhibition of paw oedema volume during the first hour. The docking study of synthesized molecule revealed a new binding pose in ATP binding pocket.
VM Chandrashekhar, SP Avinash, C Sowmya, A Ramkishan, and MH Shalavadi
Medknow
Objectives: To evaluate the neuroprotective effect of Stereospermum suaveolens DC on 6-hydroxy dopamine induced Parkinson's disease model. Materials and Methods: The study was conducted on Sprague-Dawley rats where parkinson's disease was induced by producing the striatal 6-hydroxy dopamine lesions. The test animals received methanolic extract of Stereospermum suaveolens at dose of 125, 250 and 500 mg/kg for 42 days. Behavioral assessment, spontaneous locomotor activity and muscular coordination were studied. Antioxidant levels, striatal infraction area were assessed and histopathological studies were carried out. Results: The Stereospermum suaveolens DC methanolic extract showed significant dose dependent increase in behavioral activity, improved muscular coordination. Significant reduction of lipid peroxidation (LPO), increased antioxidant enzymes like superoxide dismutase (SOD), catalase (CAT) and non-enzymatic activity of glutathione (GSH) and total thiol levels in extract treated groups was observed in test groups as compared to control group. Striatal infarction area was significantly reduced in extract treated groups as compared to control group. Conclusion: The methanolic extract of Stereospermum suaveolens DC showed neuroprotective activity against 6-hydroxy dopamine induced Parkinson's disease in rats.
Dayananda S. Badiger, Ramesh B. Nidavani, Rekha S. Hunoor, Basavaraj R. Patil, Ramesh S. Vadavi, V. M. Chandrashekhar, Iranna S. Muchchandi, and Kalagouda B. Gudasi
Wiley
A new 1,2‐dihydroquinazolinone, 2‐(2‐hydroxy‐phenyl)‐3‐[1‐(2‐oxo‐2H‐chromen‐3‐yl)‐ethylideneamino]‐2,3‐dihydro‐1H‐quinazolin‐4‐one (L) and its Mn(II), Co(II), Ni(II), Cu(II) and Zn(II) complexes have been prepared. These were characterized by elemental, spectral [UV–visible, IR, NMR (1H, 13C and 2D heteronuclear correlation) and mass], conductance, magnetic susceptibility and thermal studies. The physicochemical data indicate that the ligand behaves as tridentate with ONO donor sequence towards the metal ions, and trigonal bipyramidal geometry was assigned for complexes. The ligand and its metal complexes were evaluated for their in vivo anti‐inflammatory and analgesic activity. The tested compounds have shown excellent activity, which are almost equipotent to the standard used in the study. Copyright © 2011 John Wiley & Sons, Ltd.
V.M. Chandrashekhar, K.S. Halagali, R.B. Nidavani, M.H. Shalavadi, B.S. Biradar, D. Biswas, and I.S. Muchchandi
Elsevier BV
Prashant D. Phadatare and V. M. Chandrashekhar
Springer Science and Business Media LLC
V.L. Ranpariya, S.K. Parmar, N.R. Sheth, and V.M. Chandrashekhar
Informa UK Limited
Context: Oxidative stress plays a key role in pathophysiology of many neurodegenerative disorders such as Alzheimer’s disease, Parkinson’s disease, Huntington’s disease, and so on. Although Matricaria recutita L. (Asteraceae), German chamomile, is traditionally used for central nervous system (CNS)-related diseases, its antistress properties have received little attention. Objective: The present study evaluated the neuroprotective effect of German chamomile against aluminium fluoride (AlF4−)-induced oxidative stress in rats. Materials and methods: The Sprague-Dawley rats of either sex (200–250 g) were selected and grouped as: group I received normal saline; group II received AlF4− (negative control); groups III, IV, and V received 100, 200, and 300 mg/kg, orally, German chamomile methanol extract (GCME) along with AlF4−; and group VI received quercetin (25 mg/kg, i.p.) + AlF4−, respectively. After 10 days treatment with GCME, oxidative stress was induced by administering AlF4− through drinking water for 7 days. Then, the protective antioxidant enzyme levels were measured and the histopathological studies were carried out. Results: The GCME showed dose-dependent neuroprotective activity by significant decrease in lipid peroxidation (LPO) and increase in the superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), and total thiol levels in extract-treated animals as compared with negative control group (P < 0.001). The histopathological studies also revealed the potent neuroprotective action of German chamomile against oxidative brain damage. Conclusion: The present study for the first time shows potent neuroprotective activity of the methanol extract of German chamomile against AlF4−-induced oxidative stress in rats.
Rekha S. Hunoor, Basavaraj R. Patil, Dayananda S. Badiger, Ramesh S. Vadavi, Kalagouda B. Gudasi, V. M. Chandrashekhar, and I. S. Muchchandi
Wiley
AbstractA new quinazolinone derivative, 3‐[1‐(2‐hydroxyphenyl)ethylideamino]‐2‐phenyl‐3,4‐dihydroquinazolin‐4(3H)‐one (LH) was synthesized by the condensation of 2‐hydroxyacetophenone‐2‐aminobenzoylhydrazone and benzaldehyde. The cyclization to form 1,2‐dihydroquinazolinone was confirmed by IR, 1D and 2D HETCOR studies. Coordination compounds of Co(II), Ni(II), Cu(II) and Zn(II) of LH were synthesized and characterized using various physico‐chemical studies like stoichiometric, conductivity, magnetic moment measurements and spectral techniques such as IR, NMR, UV‐vis and EPR spectroscopy. The elemental analysis and thermal studies suggested a general stoichiometry [M(HEPDQ)Cl] for all the complexes. A four‐coordinate geometry was assigned to all the complexes. The complexes along with the parent ligand were screened for their anti‐inflammatory activity, using carrageenan‐induced rat paw edema, and for their analgesic activity by Eddy's hot plate method. The activity of the ligand was enhanced on complexation with metal ions. This enhanced activity was attributed to the increased lipophilic nature of the complexes. Notable anti‐inflammatory activity was observed for Ni(II), Cu(II) and Zn(II) complexes. The analgesic activity of the ligand was greater than the standard at 60 min. and at a 10 mg kg−1 dose, whereas the activity of Ni(II) and Cu(II) complexes at 10 mg kg−1 dose was comparable with the standard used. Copyright © 2011 John Wiley & Sons, Ltd.
Ashok A. Muchandi and V. M. Chandrashekhar
SAGE Publications
Sir, Peptic ulcer is one of the most frequent disorders of the alimentary tract and, in various countries, its prevalence is estimated at 5–10% of the adult population, still remaining one of the most important problems.[1] The etiology of peptic ulcer is not clearly known. It results probably due to an imbalance between the aggressive and the defensive factors. Reactive oxygen metabolites, free radicals, nitric oxide and genetic and environmental factors are also thought to play a role in the pathogenesis of ulcers. Although a number of antiulcer drugs, such as H2 receptor antagonists, proton pump inhibitors and cytoprotectants, are available, all these drugs have side-effects and limitations.[2] Indian medicinal plants and their derivatives have been an invaluable source of therapeutic agents with fewer side-effects to treat various disorders including peptic ulcer disease (PUD).
H.S. Yogesh, V.M. Chandrashekhar, H.R. Katti, S. Ganapaty, H.L. Raghavendra, Girish K. Gowda, and B. Goplakhrishna
Elsevier BV
Rekha S. Hunoor, Basavaraj R. Patil, Dayananda S. Badiger, Ramesh S. Vadavi, Kalagouda B. Gudasi, V.M. Chandrashekhar, and I.S. Muchchandi
Elsevier BV
V. M. Chandrashekhar, Ashok A. Muchandi, Sarasvathi. V. Sudi, and Seru. Ganapty
Informa UK Limited
The present study aims to evaluate the hepatoprotective activity of Stereospermum suaveolens DC (Bignoniaceae). Hepatoprotective activity is studied by carbon tetrachloride (CCl4)-induced liver damage in albino rats. The degree of protection in this activity has been measured by using biochemical parameters such as serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT), alkaline phosphatase (ALP), total bilirubin, LDL-cholesterol and SOD, CAT, GSH, total thiols, NO, and lipid peroxidation in liver tissue homogenate. The results suggest that the methanol stem bark extract of Stereospermum suaveolens at the doses 125, 250, and 500 mg/kg and reference standard Liv-52 treated group produced significant (p <0.001) hepatoprotection against CCl4-induced liver damage by decreasing the activities of serum enzymes, bilirubin and lipid peroxidation. The extract significantly (p <0.001) increased levels of SOD, CAT, GSH and total thiols, as compared to control group. Histopathological studies further substantiate the protective effect of the extract. It was concluded that methanol stem bark extract of Stereospermum suaveolens showed effective hepatoprotective activity.
V.M. Chandrashekhar, V.L. Ranpariya, S. Ganapaty, A. Parashar, and A.A. Muchandi
Elsevier BV
S Ganapaty, VM Chandrashekhar, HR Chitme, and MLakashmi Narsu
Medknow
Objectives: Objectives: The antioxidant potential of gossypin and nevadensin, two flavonoid compounds, were evaluated by in vitro methods. Materials and Methods: Materials and Methods: Gossypin, nevadensin, and the reference standard, butylated hydroxyl toluene (BHT), were evaluated for DPPH (1, 1-diphenyl-2-picrylhydrazyl), nitric oxide, superoxide, and hydroxyl radical scavenging activity. Results: Results: Gossypin and BHT showed the potential for significant DPPH radical inhibition of up to 88.52 and 91.45% at 100 µg/ml concentration. With a 100 µg/ml concentration of gossypin, the in vitro nitric oxide, superoxide, and hydroxyl radical scavenging activity was found to be 74.00, 74.22, and 67.15%, respectively; and with 100 µg/ml of BHT the corresponding values were 82.24, 81.76, and 73.03% of inhibition, respectively. Conclusion: Conclusion: The study results showed that gossypin has significant antioxidant activity.