@cesannauniv.in
Project Associate
Anna University
Ph.D in Environmental Biotechnology
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Albert Mariathankam Nizzy and Suruli Kannan
Springer Science and Business Media LLC
A. M. Nizzy, S. Kannan, and S. B. Anand
Springer Science and Business Media LLC
Albert Mariathankam Nizzy, Suruli Kannan, and Setty Balakrishnan Anand
Springer Science and Business Media LLC
Rahul R. Nair, P. S. Udayan, S. Thilaga, M. Kavitha, R. M. Bharathanandhini, A. M. Nizzy, and D. Ganesh
Cambridge University Press (CUP)
Morinda reticulata Gamble and Morinda umbellata Linn. (Rubiaceae) are medicinally important climbers distributed as a mixed population in southern Western Ghats of India. A close morphological resemblance of these two species misleads the harvester in the identification of plant parts for preparation of herbal medicines. Though both species contain anthraquinone derivatives and share common medicinal properties for treating stomach disorders, each of these species has unique curative properties for treating selective diseases. Conventional methods are not reliable for identification of these species due to similarities in morphology. Thus, misidentification often leads to the deterioration of the quality of medicines. Thus, authentication utilizing conserved gene sequences in the chloroplast genome of these two Morinda spp. has been attempted for precise identification. Here we report the use of two barcoding genes (maturase kinase and ribulose 1,5-bisphosphate carboxylase large subunit) to distinguish M. reticulata and M. umbellata based on single nucleotide polymorphism. The present findings can be used for authenticating leaf samples of M. reticulata and M. umbellata.
Helen PA Mary, Gomathy K Susheela, S Jayasree, AM Nizzy, B Rajagopal, and S Jeeva
Medknow
P.A. Mary Helen, Deepi Deepi, S. Jaya Sree, A.M. Nizzy, and S.M. Madoen Abisha
Oriental Scientific Publishing Company
Random amplified polymorphic DNA (RAPD) analysis was performed on 7 bacterial isolates isolated from leaf litter from canal Kollemcode. The isolates produced reproducible amplification products which were sufficiently polymorphic to allow differentiation of the strains. Five primers such as OPA-06, OPA-07, OPA-17, OPAD-02, OPAD-07 were used for the RAPD analysis. DNA banding patterns generated by RAPD were scored for the presence (1) or for absence (0) of each amplified band. For genetic distance analysis, using NTSYS software Cluster analysis was based on similarity matrices using the unweighted pair group method analysis (UPGMA) program in the software package. The Jaccard coefficient was used for dendrogram construction. A dendrogram based on these results showed a high level of genetic similarity between different bacterial isolates, and genetic differences were expressed in clusters. The genetic difference between the overall population was low and showed similarity with each population.